BTN3A1/CD277[Biotin], His & Avi, Cynomolgus
BTN3A1/CD277[Biotin], His & Avi, Cynomolgus

Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for BTN3A1/CD277[Biotin], His & Avi, Cynomolgus, His Tag with the EC50 of 2.1 ng/ml determined by ELISA.

BTN3A1/CD277[Biotin], His & Avi, Cynomolgus

The purity of BTN3A1/CD277[Biotin], His & Avi, Cynomolgus is greater than 95% as determined by SEC-HPLC.

BTN3A1/CD277[Biotin], His & Avi, Cynomolgus

BTN3A1/CD277[Biotin], His & Avi, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

BTN3A1/CD277[Biotin], His & Avi, Cynomolgus

The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
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Product Introduction
Species Cynomolgus
Protein Construction
BTN3A1/CD277 (Gln1-Trp219)
Accession # A0A330KWZ1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • BTN3A1/CD277[Biotin], His & Avi, Cynomolgus
    • BTN3A1/CD277[Biotin], His & Avi, Cynomolgus

    Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for BTN3A1/CD277[Biotin], His & Avi, Cynomolgus, His Tag with the EC50 of 2.1 ng/ml determined by ELISA.

  • BTN3A1/CD277[Biotin], His & Avi, Cynomolgus
    • BTN3A1/CD277[Biotin], His & Avi, Cynomolgus

    The purity of BTN3A1/CD277[Biotin], His & Avi, Cynomolgus is greater than 95% as determined by SEC-HPLC.

  • BTN3A1/CD277[Biotin], His & Avi, Cynomolgus
    • BTN3A1/CD277[Biotin], His & Avi, Cynomolgus

    BTN3A1/CD277[Biotin], His & Avi, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
Synonyms CD277; BTN3A1; BTF5; BT3.1; BTN3.1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.