Immobilized Human CA125, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 39.9 ng/ml determined by ELISA.

The purity of MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 18.4 ng/ml determined by ELISA.

Use MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human to detect the expression rate of Anti-MSLN-CAR positive cell. 293T cells and anti-MSLN CAR-293T cells were incubated with Biotinylated human MSLN-hFc-Avi Tag. Non-transfected 293T cells and Fc-labeled protein control were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human MSLN.

MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Cat. No.	Z03868
Size	100 μg : Quote 500 μg : Quote Buy in bulk
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Price	¥130,580.00


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Product Introduction

Species

Human

Protein Construction

MSLN/Mesothelin (Glu296-Gly580) Accession # Q13421-2	hFc	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

61.1 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

The purity of MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Target Background

Mesothelin, also known as MSLN, is encoded by the MSLN gene. It encodes a precursor protein of 71 kDa that is processed to a 31 kDa shed protein called megakaryocyte potentiating factor (MPF) and a 40 kDa fragment, mesothelin. MSLN is attached to the cell membrane by a glycosyl-phosphatidylinositol (GPI) anchor. It is a differentiation antigen which is highly expressed in several human cancers, including virtually all mesotheliomas and pancreatic adenocarcinomas.

Synonyms

MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human

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