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PiggyBac MRNA V2 (Cap1, M1Ψ)

PiggyBac MRNA V2 (Cap1, M1Ψ)

Editing performance of PiggyBac system in HEK293T cells. GC represents GenCircle, the donor DNA in GenCircle format with eGFP sequence.

PiggyBac mRNA V2 (Cap1, m1Ψ)

PiggyBac is a DNA transposon and a promising alternative to Sleeping Beauty. PiggyBac is originally isolated from the cabbage looper moth Trichoplusia ni genome, is distinguished by its ability to excise precisely. (Zheng Zhang, et al, J Comput Biol 2000; 7(1-2):203-14.)
RP-A00064
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Description

PiggyBac is a DNA transposon and a promising alternative to Sleeping Beauty. PiggyBac is originally isolated from the cabbage looper moth Trichoplusia ni genome, is distinguished by its ability to excise precisely. (Zheng Zhang, et al, J Comput Biol 2000; 7(1-2):203-14.)  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- Incorporated 2NLS peptide sequences at both the 5’ and 3’ ends of the coding region 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 2156 nt
Full mRNA Molecular Weight 695831 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 200ng of PiggyBac mRNA and 800ng of donor DNA with eGFP sequence into 5×104 HEK293T cells using Lipofectamine™ 2000 (or equivalent). On day 3, 6, 10 and 12, use flow cytometry analysis to evaluate the ratio of eGFP positive cells and eGFP expression intensity.

Examples
  • PiggyBac MRNA V2 (Cap1, M1Ψ)
    • PiggyBac MRNA V2 (Cap1, M1Ψ)

  • PiggyBac MRNA V2 (Cap1, M1Ψ)
    • PiggyBac MRNA V2 (Cap1, M1Ψ)

    Editing performance of PiggyBac system in HEK293T cells. GC represents GenCircle, the donor DNA in GenCircle format with eGFP sequence.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.