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CD19 MRNA (Cap1, M1Ψ)

CD19 MRNA (Cap1, M1Ψ)

Expression of CD19 mRNA in A549 cells. GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms.

CD19 mRNA (Cap1, m1Ψ)

CD19 is a transmembrane glycoprotein primarily expressed on B lymphocytes. It plays two major roles in human B cells: acting as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane and working within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways.
RP-A00057
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Description

CD19 mRNA is a transmembrane glycoprotein primarily expressed on B lymphocytes. It plays two major roles in human B cells: acting as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane and working within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 1939 nt
Full mRNA Molecular Weight 627740 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.5μg of CD19 mRNA into one well of cells in a 24-well plate with Lipofectamine™ MessengerMAX™ (or equivalent). 24-48 hours after transfection, detect the expression of CD19 protein using flow cytometry with staining by BD Pharmingen™ APC Mouse Anti-Human CD19 antibody (Catalog No:55541).

Examples
  • CD19 MRNA (Cap1, M1Ψ)
    • CD19 MRNA (Cap1, M1Ψ)

  • CD19 MRNA (Cap1, M1Ψ)
    • CD19 MRNA (Cap1, M1Ψ)

    Expression of CD19 mRNA in A549 cells. GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.