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TdTomato MRNA (Cap1, M1Ψ)

TdTomato MRNA (Cap1, M1Ψ)

Expression of tdTomato mRNA in A549 cells. GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms; ComP represents competitor P.

tdTomato mRNA (Cap1, m1Ψ)

tdTomato mRNA encodes for a red fluorescent protein derived from corals of the genus Discosoma. It displays excitation and emission maxima of 554 and 581 nm, respectively. tdTomato is optimized for cell and tissue imaging, with improved fluorescence intensity and stability.
RP-A00056
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Description

tdTomato mRNA encodes for a red fluorescent protein derived from corals of the genus Discosoma. It displays excitation and emission maxima of 554 and 581 nm, respectively. tdTomato is optimized for cell and tissue imaging, with improved fluorescence intensity and stability.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 1696 nt
Full mRNA Molecular Weight 550032 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.14μg of tdTomato mRNA into one well of cells in a 96-well plate with Lipofectamine™ MessengerMAX™ (or equivalent). 24-48 hours after transfection, detect the expression of tdTomato protein by plate reader with excitation at 540nm and emission at 582nm.

Examples
  • TdTomato MRNA (Cap1, M1Ψ)
    • TdTomato MRNA (Cap1, M1Ψ)

  • TdTomato MRNA (Cap1, M1Ψ)
    • TdTomato MRNA (Cap1, M1Ψ)

    Expression of tdTomato mRNA in A549 cells. GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms; ComP represents competitor P.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.