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Beta-gal MRNA (Cap1, M1Ψ)

Beta-gal MRNA (Cap1, M1Ψ)

Expression of Beta-gal mRNA in A549 cells. X4 and X5 represent GenScript mRNA codon optimized using different algorithms; ComT represents competitor T.

Beta-gal mRNA (Cap1, m1Ψ)

The LacZ β-Galactosidase (or beta-gal) is best known for its reaction with X-gal for blue/white selection and commonly used in mammalian cells as a reporter to monitor transfection efficiency and promoter activity.
RP-A00054
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Description

The LacZ β-Galactosidase (or beta-gal) can hydrolyze lactose to galactose plus glucose, transgalactosylate to form allolactose, and hydrolyze allolactose. It is best known for its reaction with X-gal (5-bromo-4-chloro-3-indoyl-β-d-galactopyranoside) for blue/white selection and commonly used in mammalian cells as a reporter to monitor transfection efficiency and promoter activity.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 3339 nt
Full mRNA Molecular Weight 1.08×10^6 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.1 µg of mRNA using 0.5 µL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96-well plate following manufacturer’s instructions. Perform Gal-Screen™ β-Galactosidase Reporter Gene Assay the next day following ThermoFisher Scientific’s assay protocol.

Examples
  • Beta-gal MRNA (Cap1, M1Ψ)
    • Beta-gal MRNA (Cap1, M1Ψ)

  • Beta-gal MRNA (Cap1, M1Ψ)
    • Beta-gal MRNA (Cap1, M1Ψ)

    Expression of Beta-gal mRNA in A549 cells. X4 and X5 represent GenScript mRNA codon optimized using different algorithms; ComT represents competitor T.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.