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ABE8e MRNA (Cap1, M1Ψ)

ABE8e MRNA (Cap1, M1Ψ)

ABE8e mRNA and EXM1 sgRNA encapsulated with either SM102 or SM102-Mod LNPs were able to mediate efficient base editing at A3, A5, and A6 sites. However, bystander editing was observed at the A7 site. The editing efficiency of the SM102-Mod LNP group was higher than that of the SM102 LNP group.

ABE8e mRNA (Cap1, m1Ψ)

Adenine base editors (ABEs) system is constructed by fusing an artificially evolved adenine deaminase to a mutated Cas9 that is a single-strand DNA nickase, can precisely and permanently convert A·T to G·C with the guidance of a target-specific guide RNA (gRNA) without creating a double-strand DNA break and without requiring an exogenous DNA repair donor.
RP-A00053
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Description

Adenine base editors (ABEs) system is constructed by fusing an artificially evolved adenine deaminase to a mutated Cas9 that is a single-strand DNA nickase, can precisely and permanently convert A·T to G·C with the guidance of a target-specific guide RNA (gRNA) without creating a double-strand DNA break and without requiring an exogenous DNA repair donor. This mRNA is human codon optimized ABE8e, with sequence originally from Richter, M.F. et al Nature Biotechnology volume 38, pages883–891 (2020). 
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 5084 nt
Full mRNA Molecular Weight 1656299 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.5 µg of ABE8e mRNA and sgRNA (at a 2:1 mass ratio) into 2×10⁵ HEK293T cells (through LNP encapsulation, electroporation, or transfection reagent). 48 hours after transfection, extract genomic DNA, and amplify the target region by PCR. Analyze the PCR products through Sanger sequencing and EditR tool.

Examples
  • ABE8e MRNA (Cap1, M1Ψ)
    • ABE8e MRNA (Cap1, M1Ψ)

  • ABE8e MRNA (Cap1, M1Ψ)
    • ABE8e MRNA (Cap1, M1Ψ)

    ABE8e mRNA and EXM1 sgRNA encapsulated with either SM102 or SM102-Mod LNPs were able to mediate efficient base editing at A3, A5, and A6 sites. However, bystander editing was observed at the A7 site. The editing efficiency of the SM102-Mod LNP group was higher than that of the SM102 LNP group.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.