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Rluc MRNA (Cap1, M1Ψ)

Rluc MRNA (Cap1, M1Ψ)

Expression of Rluc mRNA in A549 cells. GS Rluc GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms; ComT represents competitor T.

Rluc mRNA (Cap1, m1Ψ)

Renilla luciferase (Rluc) produces blue light with a peak emission at 480nm. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudouridine (m1Ψ) for enhanced expression and reduced immunogenicity. It has been codon optimized with GenScript X5 algorithm for enhancing its expression efficiency. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.
RP-A00052
¥63,000.00

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Description

Renilla luciferase is a bioluminescent enzyme derived from the sea pansy Renilla reniformis, that catalyzes the oxidation of coelenterazine, producing blue light with a peak emission at 480nm.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 1200 nt
Full mRNA Molecular Weight 388949 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.1 μg of mRNA using 0.5 μL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96-well plate following manufacture’s instruction. Perform Rluc assay the next day following Promega’s Rluc assay protocol.

Examples
  • Rluc MRNA (Cap1, M1Ψ)
    • Rluc MRNA (Cap1, M1Ψ)

  • Rluc MRNA (Cap1, M1Ψ)
    • Rluc MRNA (Cap1, M1Ψ)

    Expression of Rluc mRNA in A549 cells. GS Rluc GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms; ComT represents competitor T.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.