ホーム » カタログRNA＆LNP製品 » hEPO mRNA(Cap1, m1Ψ)

hEPO mRNA(Cap1, m1Ψ)

This product is unavailable.
Erythropoietin (EPO) is a glycoprotein hormone mainly secreted by the kidney that promotes red blood cell production and maintains oxygen supply. Recombinant human EPO protein has been widely used to treat anemias resulting from kidney diseases and cancer chemotherapy. This mRNA is capped with Cap1 structure with high capping efficiency. It is 100% substituted with N1-methyl-pseudouridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, which mimics a mature mRNA.
RP-A00034
Ask us a question
Description

Erythropoietin is a hormone essential for the production of red blood cells and for maintaining oxygen supply to the body. It is a glycoprotein secreted mainly by the kidney and also by the liver. Recombinant human EPO protein has been widely used to treat anemias resulting from kidney diseases and cancer chemotherapy.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 846nt
Full mRNA Molecular Weight 273190
Storage buffer 1mM Sodium citrate, pH6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.1 ug of mRNA using 0.3 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96-well plate following manufacturer’s instruction. Collect the cell supernatant after 12 to 16 hours. Cell supernatant was diluted 5x using sample dilution buffer, and EPO secretion was measured using the ELISA kit Human EPO (Sandwich ELISA) ELISA Kit - BMS2035-2 from ThermoFisher.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.