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F-Luc MRNA (Cap1, 5-MOU)

F-Luc MRNA (Cap1, 5-MOU)

Expression of F-Luc mRNA (5-MOU) in A549 cells. The expression was measured 24 and 48 hours after transfection of mRNA by luciferase assay (Promega, E4030). m1Ψ modified F-Luc mRNA was utilized as a control.

F-Luc mRNA (Cap1, 5-MOU)

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The Firefly-Luciferase (F-Luc) mRNA expresses firefly luciferase protein, which is the enzyme for bioluminescence of fireflies and click beetles. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with 5-Methoxyuridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA. After efficient delivery into cells or animals, F-Luc mRNA can translate into F-Luc and emit bioluminescence with Luciferin.
RP-A00022
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Description

The Firefly Luciferase (F-Luc) mRNA encodes the enzyme responsible for the bioluminescence seen in fireflies and click beetles. Upon delivery into cells or animals, it is translated into F-Luc protein, which emits light in the presence of its substrate, Luciferin.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with 5-Methoxyuridine (5-MOU) for improved protein expression and reduced innate immune response 
- A 100A poly(A) tail to mimic natural mature mRNA 
 
This construct offers strong, reliable bioluminescent signaling, making it ideal for transfection optimization, biodistribution studies, and LNP delivery validation.
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 1914nt
Full mRNA Molecular Weight 622225
Storage buffer 1mM Sodium citrate, pH6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.1-0.5 µg of mRNA with 0.5 µL of Lipofectamine™ MessengerMAX™ (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s instructions. Assess luciferase expression 12-48 hours post-transfection using luciferase assay system (Promega, E4030).

Examples
  • F-Luc MRNA (Cap1, 5-MOU)
    • F-Luc MRNA (Cap1, 5-MOU)

  • F-Luc MRNA (Cap1, 5-MOU)
    • F-Luc MRNA (Cap1, 5-MOU)

    Expression of F-Luc mRNA (5-MOU) in A549 cells. The expression was measured 24 and 48 hours after transfection of mRNA by luciferase assay (Promega, E4030). m1Ψ modified F-Luc mRNA was utilized as a control.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.