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GenLNP-A01-EGFP mRNA (m1Ψ)

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The LNP formulation loaded with eGFP mRNA (m1Ψ) is prepared using generic LNP formulation with main ionizable lipid of ALC0315. The payload EGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudoUridine. It expresses an enhanced green fluorescent protein (Ex: 488nm, Em: 507nm). This formulation is a good control for testing if ALC0315 LNP is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model.
RP-A00010
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Description

The LNP formulation loaded with eGFP mRNA (m1Ψ) is prepared using generic LNP formulation with main ionizable lipid of ALC0315.  
 
The payload EGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). It expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP mRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects. The expressed protein has an excitation wavelength of 488nm, emission at 507nm.  
 
This formulation is a good control for testing if ALC0315 LNP formulation is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model.
Properties
Form Liquid
Concentration 0.15mg/mL
Full mRNA length 980nt
Full mRNA Molecular Weight 319792
Storage buffer Tris-HCl/Sucrose
Storage condition Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.

Quality Control Specifications
Appearance Clear and free of foreign particles
Encapsulation Efficiency > 85%
Endotoxin < 4 EU/ml
Particle Size 50-100 nm
pH 7.4 ± 0.5​
Polydispersity Index < 0.2
Zeta Potential ± 15.0 mV

Applications
1. LNP can be added directly to cells incubated in complete media 
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium 
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate  
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression 
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.