安定細胞株製品 » Human Recombinant PAR4 Proteinase-activated Receptor Stable Cell Line
CHO-K1/PAR4 Stable Cell Line

Figure 1. Trypsin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PAR4 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to being stimulated with agonist trypsin. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of trypsin (Mean ± SEM, n = 3). The EC50 of trypsin on this cell was 0.61 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50 -X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/PAR4 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human protease-activated receptor 4 (PAR4) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00206
¥1,661,121.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human protease-activated receptor 4 (PAR4) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/PAR4 Stable Cell Line
    • CHO-K1/PAR4 Stable Cell Line

    Figure 1. Trypsin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PAR4 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to being stimulated with agonist trypsin. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of trypsin (Mean ± SEM, n = 3). The EC50 of trypsin on this cell was 0.61 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50 -X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.