Protein Isolation and Analysis » タンパク質単離・精製 » 精製用レジン » Protein A Resin FF Prepacked Column

Protein A Resin FF Prepacked Column

GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens.
L00680
¥35,391.00

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Overview
Product description GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc.
Key Features ● Static binding capacity >40 mg human IgG/ml settled resin
● Recombinant protein A with non-essential domains removed
● Fab region available for binding antigens
● Compatible with ÄKTA™ chromatography systems

Properties
Formulation 50% slurry in 20% ethanol
Matrix 4% highly cross-linked agarose
Average Particle Size 90 μm (45-165 μm)
Ligand Recombinant Streptococcal protein A expressed in E. coli
Number of binding sites per ligand 5
M.W. of ligand Approximately 34 kDa
PI of ligand 5.17
Binding Capacity > 40 mg human IgG/ ml settled resin
Degree of substitution Approximately 5 mg protein A / ml settled resin
Application purification of classes, subclasses and fragments of immunoglobulins;
immunoprecipitation of proteins, protein complexes or antigens
Storage Stored at 2°C~8°C. Do not freeze.


For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.