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Ni-charged MagBeads

Ni-charged MagBeads

Ni-charged MagBeads

The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.
L00295
¥14,140.00

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Description

The GenScript Ni-Charged MagBeads are developed for_x000D_ quick and efficient small-scale purification of polyhistidine-tagged_x000D_ proteins under native or denaturing conditions.

Perform cell lysis under native or denaturing conditions. Add the cell_x000D_ lysate containing your polyhistidine-tagged_x000D_ proteins to the Ni-Charged MagBeads and allow the proteins bind to the MagBeads. Then_x000D_ the isolated proteins can be eluted from the beads. Magnetic separation eliminates the need for_x000D_ multiple tubes, minimizes the loss of sample and removes several steps of the_x000D_ centrifugation process. 
The GenScript Ni-Charged_x000D_ MagBeads are average 40 µm in size, super paramagnetic beads with strong metal-chelating agent covalently bound to their_x000D_ surfaces. They are pre-charged with nickel and ready to use for quick and_x000D_ small-scale purification of polyhistidine-tagged proteins. The beads are_x000D_ supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing_x000D_ 20% ethanol and 1 mM NiSO4. The Ni-Charged MagBeads_x000D_ have a binding capacity of 40 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4_x000D_ mL 25% slurry).
Overview
Key Features
Key Features
  • Quick and convenient separation accomplished by magnetic force
  • High binding capacity with 40 mg of 6xHis-tagged protein/ml settled beads
  • Compatible with various reagents needed in the purification process (see table 1)
  • Can be regenerated for multiple uses
  • Extremely low nonspecific binding


Table 1 Reagents Compatible with Ni-charged Magnetic Beads

Denaturants Detergents Reducing agents Salts Others
6 M Gu•HCl 2% Triton X-100 20 mM β-ME 4 M MgCl2 50% glycerol
8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl2 20% ethanol
1% CHAPS 2 M NaCl 1 mM EDTA

Properties
Storage This product is stable until the expiration date stated on the COA, when stored unopened at 2–8°C. Do NOT freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination. 

Examples
  • Ni-charged MagBeads
    • Ni-charged MagBeads

  • Ni-charged MagBeads
    • Ni-charged MagBeads