Protein A/G MagBeads

Intended Use
GenScript Protein A/G MagBeads are ideal for small‐ scale antibody purification and immunoprecipitation (IP) of proteins, protein complexes or other antigens.

Principle
The sample containing antibody is added to the Protein A/G MagBeads. The antibody will bind to beads during a short incubation. Then the beads-bound antibody may be eluted from the beads by using a magnetic separation rack, or used for immunoprecipitation (IP). A cross-linking procedure may be needed before IP to prevent co-elution of the primary antibody. Magnetic separation eliminates the changes of micro tubes, minimizes the loss of sample and removes excessive steps of traditional centrifugation method.

Material Supplied
GenScript Protein A/G MagBeads are super paramagnetic beads of average 40 μm in diameter, covalently coated with recombinant Protein A/G. The beads are supplied as 25% slurry in 20% ethanol. The Protein A/G MagBeads have a binding capacity of more than 10 mg Human IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6× His‐ tag was attached to its N‐ terminal to facilitate the purification. The secreted Protein A/G contains four Fc‐ binding domains from Protein A and two from Protein G, making it a more universal tool to bind and purify immunoglobulins.

Storage and Stability
This product is stable until the expiration date stated on the COA, when stored unopened at 2-8°C. Do not freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination.