ube3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube3b
Entrez Gene ID	780337
Full Name	ubiquitin protein ligase E3B
General protein information	Preferred Names ubiquitin protein ligase E3B Names ubiquitin-protein ligase E3B HECT-type ubiquitin transferase E3B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012966190.3	XP_012821644.1	ubiquitin-protein ligase E3B isoform X1
NM_001079407.1	NP_001072875.1	ubiquitin-protein ligase E3B
XM_031902387.1	XP_031758247.1	ubiquitin-protein ligase E3B isoform X1
XM_031902395.1	XP_031758255.1	ubiquitin-protein ligase E3B isoform X1
XM_012966190.2	XP_012821644.1	ubiquitin-protein ligase E3B isoform X1
XM_018094690.1	XP_017950179.1	ubiquitin-protein ligase E3B isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ube3b	NP_001072875.1
Pan troglodytes (chimpanzee)	UBE3B	XP_001137003.2
Danio rerio (zebrafish)	ube3b	NP_001108154.1
	UBE3B	XP_002798818.1
Homo sapiens (human)	UBE3B	NP_569733.2
Canis lupus familiaris (dog)	UBE3B	XP_543437.2
Bos taurus (cattle)	UBE3B	NP_001193161.1
Drosophila melanogaster (fruit fly)	CG5087	NP_001261610.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005897	XP_315926.2
Caenorhabditis elegans (roundworm)	oxi-1	NP_499392.1
Mus musculus (house mouse)	Ube3b	NP_473434.2
Rattus norvegicus (Norway rat)	Ube3b	NP_001137366.1
Gallus gallus (chicken)	LOC776286	XP_003642246.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ube3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa41882	XM_018094690.1 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa00604	XM_012966190.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa00604	XM_031902395.1 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa00604	XM_031902387.1 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa00604	NM_001079407.1 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa00604	XM_012966190.2	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube3b ( XM_018094690.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950179.1 Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa41882
Clone ID Related Accession (Same CDS sequence)	XM_018094690.1
Accession Version	XM_018094690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTCA CCTTTACAGA TACCCTAACC TGGAAAATAC TCAAGGGGAA AGGCGAAAGC 
CTGAGACCAG CTATGAACCA CATTTGTGCA AATATTATGG GGTATCTTAA CCAGAAAGGA 
TTTTATTCTG TTCTACAGAT ACTGTTAACC AATGGCTTGG CCAGATCTCG CCCCTCCTTG 
TCCAAAGGCA CGCTTACAGC CCTGTTTTCA TTGGCGTTGC GTCCAGTAGT AGCAGCACAA 
TATTCTGACA ACCTTCTTCG ATCCTTCTTG ATCCACATAA TGTCTGTACC AGCTTTGGTT 
TCCCACTTGT CTACACTTAC TCCAGACAGG CTTTCCGTAA TAGAATCGAA TGGTCTCTTC 
CATAAATTCA TCTTGTTTCT GAGCCGGGAG GATCAGTGCA GTGACGTGTG TGAGTGCCTG 
GAGGGCAGCC ATACTCTTTG TTTGCTAGGT AACCTCATTC ACCTGGGCCA CCTGACAGAG 
AAGGTGTTGG AGGAGGAGAT GTTTCACTTT GTCAGTGTCC TTACCCAAAT GTTATCTTAT 
TGCCAGAAAT ATGTGTCTCA GAAGAAGTCC AATCTTACAC ACTGGCACCC TGTTCTGGGC 
TGGTTCTCCC AGACAGTGGA CTATGGTCTC AATGAGTCCA TGCCCTTACT CACCAAGCAA 
CTTCAACATC TGTGGGGTGT CCATATGATA CGTAATTTGT TTCGTGAGGT CCTAAGCAAG 
AAGTTGGCAG AAAACCAAGA CGTGTCTGTT CCTGTGTCAC AAAGCTCTTC TTCCTCCCCA 
CAGAATAATC TACCCATGAA GAATCTCTTT AAACGAGCGT TCCAGAAATC TGCTTCTGTT 
CGACACATCC TTAAACCTAT TGGGGGGAAA AAGGTAGATT CTCCAGAAGT GCAAAAAGTT 
TGCAACATCT GTGTTCTGTA CCAGATGGCA CTGACAACTC TGACACAGAT ACGGCTACAG 
ATCCTCACAG GGCTTACCTA TCTGGATGAC CTTTTGCCCA AGTTGTGGGC ATTCATATGT 
GAACTAGGAC CTCAGGGAGG TCTGAAACTC TTCTTGGAAT GTCTGAGTAG TGACACAGAG 
GAGTCCCGTA GACTTCTGGC TATGCTGGTT TTGTTTTGCG ACTGCTCTAG GCATTTAATT 
ACGATCTTGG ATGATATTGA GGTATATGAA GAACAGATAT CATTTAAACT AGAGGAGCTT 
GTGACTATTT CCTCCTTTCT GAACTCCTTT GTGTTCAAGA TGATCTGGGA TGGACTTCTT 
GAGAATGCCA AAGGAGAGAC TCTGGAGCTA TTCCACTCTG TGCATGGATG GCTCATGGTC 
CTGTATGAAA GAGACTGCCG TAGGCGTTTT GCCCCTGAGG ATCATTGGCT GCGCAAGGAT 
CTCAAACCAA CCCTGCTATT CCAAGAACTG GATAAAGAGA GAAAGAGAGC CCAGCTCTTA 
CTTCAGTATA TCCCTCATGT CATCCCACAC AAAAACAGGG TCCTCTTGTT CCGAAATATT 
GTCACCAAGG AAAAGGAGAA GTTGGGTTTA GTGGAAACTA ACACCGCATC CCCACATGTC 
ACCCACATTA CCATCCGAAG GTCACGTATG TTAGAGGATG GATACGACCA ACTAAGGCAG 
CTTTCCCAGA ATGCCATGAA AGGTGTTATA CGTGTAAAGT TTGTGAACGA TCTTGGTGTA 
GATGAAGCTG GTATAGACCA GGATGGTGTC TTTAAGGAGT TCTTAGAGGA AATTATCAAG 
AGAGTATTTG ACCCTGCACT GAATCTTTTT AAGACCACTA GTTCTGGAGA TAGGTTGTAC 
CCATCTCCTA CATCTTATAT ACATGAAAAC TACCTCCAGC TGTTTGAATT TGTAGGGAAG 
ATGCTGGGCA AAGCAGTGTA TGAGGGTATA GTGGTGGATG TCCCATTTGC ATCATTCTTT 
CTCAGCCAGC TTCTTGGGCA CCATCATAGC ATCTTCTACA GCTCTGTGGA TGAGCTGCCT 
TCCTTGGACT CTGAATTCTA CAAGAATCTC ACTTCCATCA AGCGCTACGA TGGGGATATT 
AGTGATTTGG GACTTACCCT TTCATACGAT GAGGATGTTA TGGGTCAGCT GGTCTGTCAT 
GAACTGGTCC CAGGTGGGAA GTCTATTCCA GTGACTAATG AAAATAAAAT CAGTTATATC 
CACCTCATGG CTCATTTCCG CATGCACACG CAGATAAAGA ACCAAACTGT TGCCTTCATT 
AGTGGATTCC GCTCCATCAT AAGGCCTGAA TGGATCCGCA TGTTCTCAGC ACCTGAACTG 
CAACGTCTCA TATCAGGGGA CAATGCAGAG ATTGACCTGG ATGATCTAAA GAAACACACA 
GTCTACTATG GAGGGTTTCA TGGAAGTCAT CGTGTCATTA TCTGGCTGTG GGATATTTTG 
GCCAATGATT TCTCTTCTGA AGAAAAAGCC ATGTTTCTTA AGTTTGTCAC CAGTTGCTCT 
CGGCCTCCTC TACTTGGATT TGCCTACCTC AAACCTCCCT TTTCCATTAG ATGTGTAGAG 
GTGTCAGATG ATCAGGATAC AGGCGACACC CTGGGCAGTG TCCTACGTGG GTTCTTCACC 
ATCCGAAAGA AAGAGCCTGG TGGACGCCTT CCCACTTCCT CCACCTGCTT CAATCTCCTG 
AAGCTTCCCA ACTACAGTAA AAAAGGAATT CTGAGGGAGA AACTACGCTA TGCTATCAGC 
ATGAACACTG GTTTTGAGCT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950179.1
CDS	129..2855
Translation	MLVTFTDTLTWKILKGKGESLRPAMNHICANIMGYLNQKGFYSVLQILLTNGLARSRPSLSKGTLTALFSLALRPVVAAQYSDNLLRSFLIHIMSVPALVSHLSTLTPDRLSVIESNGLFHKFILFLSREDQCSDVCECLEGSHTLCLLGNLIHLGHLTEKVLEEEMFHFVSVLTQMLSYCQKYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLLTKQLQHLWGVHMIRNLFREVLSKKLAENQDVSVPVSQSSSSSPQNNLPMKNLFKRAFQKSASVRHILKPIGGKKVDSPEVQKVCNICVLYQMALTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPQGGLKLFLECLSSDTEESRRLLAMLVLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGLLENAKGETLELFHSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPTLLFQELDKERKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKEKLGLVETNTASPHVTHITIRRSRMLEDGYDQLRQLSQNAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSSGDRLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSIFYSSVDELPSLDSEFYKNLTSIKRYDGDISDLGLTLSYDEDVMGQLVCHELVPGGKSIPVTNENKISYIHLMAHFRMHTQIKNQTVAFISGFRSIIRPEWIRMFSAPELQRLISGDNAEIDLDDLKKHTVYYGGFHGSHRVIIWLWDILANDFSSEEKAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKGILREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_018094690.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094690.1

ORF Insert Sequence:

ATGCTGGTCA CCTTTACAGA TACCCTAACC TGGAAAATAC TCAAGGGGAA AGGCGAAAGC 
CTGAGACCAG CTATGAACCA CATTTGTGCA AATATTATGG GGTATCTTAA CCAGAAAGGA 
TTTTATTCTG TTCTACAGAT ACTGTTAACC AATGGCTTGG CCAGATCTCG CCCCTCCTTG 
TCCAAAGGCA CGCTTACAGC CCTGTTTTCA TTGGCGTTGC GTCCAGTAGT AGCAGCACAA 
TATTCTGACA ACCTTCTTCG ATCCTTCTTG ATCCACATAA TGTCTGTACC AGCTTTGGTT 
TCCCACTTGT CTACACTTAC TCCAGACAGG CTTTCCGTAA TAGAATCGAA TGGTCTCTTC 
CATAAATTCA TCTTGTTTCT GAGCCGGGAG GATCAGTGCA GTGACGTGTG TGAGTGCCTG 
GAGGGCAGCC ATACTCTTTG TTTGCTAGGT AACCTCATTC ACCTGGGCCA CCTGACAGAG 
AAGGTGTTGG AGGAGGAGAT GTTTCACTTT GTCAGTGTCC TTACCCAAAT GTTATCTTAT 
TGCCAGAAAT ATGTGTCTCA GAAGAAGTCC AATCTTACAC ACTGGCACCC TGTTCTGGGC 
TGGTTCTCCC AGACAGTGGA CTATGGTCTC AATGAGTCCA TGCCCTTACT CACCAAGCAA 
CTTCAACATC TGTGGGGTGT CCATATGATA CGTAATTTGT TTCGTGAGGT CCTAAGCAAG 
AAGTTGGCAG AAAACCAAGA CGTGTCTGTT CCTGTGTCAC AAAGCTCTTC TTCCTCCCCA 
CAGAATAATC TACCCATGAA GAATCTCTTT AAACGAGCGT TCCAGAAATC TGCTTCTGTT 
CGACACATCC TTAAACCTAT TGGGGGGAAA AAGGTAGATT CTCCAGAAGT GCAAAAAGTT 
TGCAACATCT GTGTTCTGTA CCAGATGGCA CTGACAACTC TGACACAGAT ACGGCTACAG 
ATCCTCACAG GGCTTACCTA TCTGGATGAC CTTTTGCCCA AGTTGTGGGC ATTCATATGT 
GAACTAGGAC CTCAGGGAGG TCTGAAACTC TTCTTGGAAT GTCTGAGTAG TGACACAGAG 
GAGTCCCGTA GACTTCTGGC TATGCTGGTT TTGTTTTGCG ACTGCTCTAG GCATTTAATT 
ACGATCTTGG ATGATATTGA GGTATATGAA GAACAGATAT CATTTAAACT AGAGGAGCTT 
GTGACTATTT CCTCCTTTCT GAACTCCTTT GTGTTCAAGA TGATCTGGGA TGGACTTCTT 
GAGAATGCCA AAGGAGAGAC TCTGGAGCTA TTCCACTCTG TGCATGGATG GCTCATGGTC 
CTGTATGAAA GAGACTGCCG TAGGCGTTTT GCCCCTGAGG ATCATTGGCT GCGCAAGGAT 
CTCAAACCAA CCCTGCTATT CCAAGAACTG GATAAAGAGA GAAAGAGAGC CCAGCTCTTA 
CTTCAGTATA TCCCTCATGT CATCCCACAC AAAAACAGGG TCCTCTTGTT CCGAAATATT 
GTCACCAAGG AAAAGGAGAA GTTGGGTTTA GTGGAAACTA ACACCGCATC CCCACATGTC 
ACCCACATTA CCATCCGAAG GTCACGTATG TTAGAGGATG GATACGACCA ACTAAGGCAG 
CTTTCCCAGA ATGCCATGAA AGGTGTTATA CGTGTAAAGT TTGTGAACGA TCTTGGTGTA 
GATGAAGCTG GTATAGACCA GGATGGTGTC TTTAAGGAGT TCTTAGAGGA AATTATCAAG 
AGAGTATTTG ACCCTGCACT GAATCTTTTT AAGACCACTA GTTCTGGAGA TAGGTTGTAC 
CCATCTCCTA CATCTTATAT ACATGAAAAC TACCTCCAGC TGTTTGAATT TGTAGGGAAG 
ATGCTGGGCA AAGCAGTGTA TGAGGGTATA GTGGTGGATG TCCCATTTGC ATCATTCTTT 
CTCAGCCAGC TTCTTGGGCA CCATCATAGC ATCTTCTACA GCTCTGTGGA TGAGCTGCCT 
TCCTTGGACT CTGAATTCTA CAAGAATCTC ACTTCCATCA AGCGCTACGA TGGGGATATT 
AGTGATTTGG GACTTACCCT TTCATACGAT GAGGATGTTA TGGGTCAGCT GGTCTGTCAT 
GAACTGGTCC CAGGTGGGAA GTCTATTCCA GTGACTAATG AAAATAAAAT CAGTTATATC 
CACCTCATGG CTCATTTCCG CATGCACACG CAGATAAAGA ACCAAACTGT TGCCTTCATT 
AGTGGATTCC GCTCCATCAT AAGGCCTGAA TGGATCCGCA TGTTCTCAGC ACCTGAACTG 
CAACGTCTCA TATCAGGGGA CAATGCAGAG ATTGACCTGG ATGATCTAAA GAAACACACA 
GTCTACTATG GAGGGTTTCA TGGAAGTCAT CGTGTCATTA TCTGGCTGTG GGATATTTTG 
GCCAATGATT TCTCTTCTGA AGAAAAAGCC ATGTTTCTTA AGTTTGTCAC CAGTTGCTCT 
CGGCCTCCTC TACTTGGATT TGCCTACCTC AAACCTCCCT TTTCCATTAG ATGTGTAGAG 
GTGTCAGATG ATCAGGATAC AGGCGACACC CTGGGCAGTG TCCTACGTGG GTTCTTCACC 
ATCCGAAAGA AAGAGCCTGG TGGACGCCTT CCCACTTCCT CCACCTGCTT CAATCTCCTG 
AAGCTTCCCA ACTACAGTAA AAAAGGAATT CTGAGGGAGA AACTACGCTA TGCTATCAGC 
ATGAACACTG GTTTTGAGCT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( XM_012966190.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821644.1 Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa00604
Clone ID Related Accession (Same CDS sequence)	XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
Accession Version	XM_012966190.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821644.1
CDS	330..3548
Translation	MFGATQVSKAQFLDKARQAREERKGLKERDRAAIKIQALIRRFLCRCRLQKEIRQEVDDFLEGTEKGSVKANALSIFRISRKLLIIFQISQDRMRFEKLCRCILNSMENDNEPKVWFVSLALSKDLTLLWIKQIKDILWYCSEYLKQLKPDILQDSKLITVYLTMLVTFTDTLTWKILKGKGESLRPAMNHICANIMGYLNQKGFYSVLQILLTNGLARSRPSLSKGTLTALFSLALRPVVAAQYSDNLLRSFLIHIMSVPALVSHLSTLTPDRLSVIESNGLFHKFILFLSREDQCSDVCECLEGSHTLCLLGNLIHLGHLTEKVLEEEMFHFVSVLTQMLSYCQKYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLLTKQLQHLWGVHMIRNLFREVLSKKLAENQDVSVPVSQSSSSSPQNNLPMKNLFKRAFQKSASVRHILKPIGGKKVDSPEVQKVCNICVLYQMALTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPQGGLKLFLECLSSDTEESRRLLAMLVLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGLLENAKGETLELFHSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPTLLFQELDKERKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKEKLGLVETNTASPHVTHITIRRSRMLEDGYDQLRQLSQNAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSSGDRLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSIFYSSVDELPSLDSEFYKNLTSIKRYDGDISDLGLTLSYDEDVMGQLVCHELVPGGKSIPVTNENKISYIHLMAHFRMHTQIKNQTVAFISGFRSIIRPEWIRMFSAPELQRLISGDNAEIDLDDLKKHTVYYGGFHGSHRVIIWLWDILANDFSSEEKAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKGILREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_012966190.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966190.3

ORF Insert Sequence:

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( XM_031902387.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758247.1 Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa00604
Clone ID Related Accession (Same CDS sequence)	XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
Accession Version	XM_031902387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758247.1
CDS	476..3694
Translation	MFGATQVSKAQFLDKARQAREERKGLKERDRAAIKIQALIRRFLCRCRLQKEIRQEVDDFLEGTEKGSVKANALSIFRISRKLLIIFQISQDRMRFEKLCRCILNSMENDNEPKVWFVSLALSKDLTLLWIKQIKDILWYCSEYLKQLKPDILQDSKLITVYLTMLVTFTDTLTWKILKGKGESLRPAMNHICANIMGYLNQKGFYSVLQILLTNGLARSRPSLSKGTLTALFSLALRPVVAAQYSDNLLRSFLIHIMSVPALVSHLSTLTPDRLSVIESNGLFHKFILFLSREDQCSDVCECLEGSHTLCLLGNLIHLGHLTEKVLEEEMFHFVSVLTQMLSYCQKYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLLTKQLQHLWGVHMIRNLFREVLSKKLAENQDVSVPVSQSSSSSPQNNLPMKNLFKRAFQKSASVRHILKPIGGKKVDSPEVQKVCNICVLYQMALTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPQGGLKLFLECLSSDTEESRRLLAMLVLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGLLENAKGETLELFHSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPTLLFQELDKERKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKEKLGLVETNTASPHVTHITIRRSRMLEDGYDQLRQLSQNAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSSGDRLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSIFYSSVDELPSLDSEFYKNLTSIKRYDGDISDLGLTLSYDEDVMGQLVCHELVPGGKSIPVTNENKISYIHLMAHFRMHTQIKNQTVAFISGFRSIIRPEWIRMFSAPELQRLISGDNAEIDLDDLKKHTVYYGGFHGSHRVIIWLWDILANDFSSEEKAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKGILREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_031902387.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902387.1

ORF Insert Sequence:

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( XM_031902395.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758255.1 Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa00604
Clone ID Related Accession (Same CDS sequence)	XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
Accession Version	XM_031902395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758255.1
CDS	253..3471
Translation	MFGATQVSKAQFLDKARQAREERKGLKERDRAAIKIQALIRRFLCRCRLQKEIRQEVDDFLEGTEKGSVKANALSIFRISRKLLIIFQISQDRMRFEKLCRCILNSMENDNEPKVWFVSLALSKDLTLLWIKQIKDILWYCSEYLKQLKPDILQDSKLITVYLTMLVTFTDTLTWKILKGKGESLRPAMNHICANIMGYLNQKGFYSVLQILLTNGLARSRPSLSKGTLTALFSLALRPVVAAQYSDNLLRSFLIHIMSVPALVSHLSTLTPDRLSVIESNGLFHKFILFLSREDQCSDVCECLEGSHTLCLLGNLIHLGHLTEKVLEEEMFHFVSVLTQMLSYCQKYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLLTKQLQHLWGVHMIRNLFREVLSKKLAENQDVSVPVSQSSSSSPQNNLPMKNLFKRAFQKSASVRHILKPIGGKKVDSPEVQKVCNICVLYQMALTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPQGGLKLFLECLSSDTEESRRLLAMLVLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGLLENAKGETLELFHSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPTLLFQELDKERKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKEKLGLVETNTASPHVTHITIRRSRMLEDGYDQLRQLSQNAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSSGDRLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSIFYSSVDELPSLDSEFYKNLTSIKRYDGDISDLGLTLSYDEDVMGQLVCHELVPGGKSIPVTNENKISYIHLMAHFRMHTQIKNQTVAFISGFRSIIRPEWIRMFSAPELQRLISGDNAEIDLDDLKKHTVYYGGFHGSHRVIIWLWDILANDFSSEEKAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKGILREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_031902395.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902395.1

ORF Insert Sequence:

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( NM_001079407.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072875.1 Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), mRNA.

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CloneID	OXa00604
Clone ID Related Accession (Same CDS sequence)	XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
Accession Version	NM_001079407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124027.1. ##Evidence-Data-START## Transcript exon combination :: BC124027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072875.1
CDS	100..3318
Translation	MFGATQVSKAQFLDKARQAREERKGLKERDRAAIKIQALIRRFLCRCRLQKEIRQEVDDFLEGTEKGSVKANALSIFRISRKLLIIFQISQDRMRFEKLCRCILNSMENDNEPKVWFVSLALSKDLTLLWIKQIKDILWYCSEYLKQLKPDILQDSKLITVYLTMLVTFTDTLTWKILKGKGESLRPAMNHICANIMGYLNQKGFYSVLQILLTNGLARSRPSLSKGTLTALFSLALRPVVAAQYSDNLLRSFLIHIMSVPALVSHLSTLTPDRLSVIESNGLFHKFILFLSREDQCSDVCECLEGSHTLCLLGNLIHLGHLTEKVLEEEMFHFVSVLTQMLSYCQKYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLLTKQLQHLWGVHMIRNLFREVLSKKLAENQDVSVPVSQSSSSSPQNNLPMKNLFKRAFQKSASVRHILKPIGGKKVDSPEVQKVCNICVLYQMALTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPQGGLKLFLECLSSDTEESRRLLAMLVLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGLLENAKGETLELFHSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPTLLFQELDKERKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKEKLGLVETNTASPHVTHITIRRSRMLEDGYDQLRQLSQNAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSSGDRLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSIFYSSVDELPSLDSEFYKNLTSIKRYDGDISDLGLTLSYDEDVMGQLVCHELVPGGKSIPVTNENKISYIHLMAHFRMHTQIKNQTVAFISGFRSIIRPEWIRMFSAPELQRLISGDNAEIDLDDLKKHTVYYGGFHGSHRVIIWLWDILANDFSSEEKAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKGILREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_001079407.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079407.1

ORF Insert Sequence:

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3b ( XM_012966190.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821644.1 Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

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CloneID	OXa00604
Clone ID Related Accession (Same CDS sequence)	XM_012966190.3 , NM_001079407.1 , XM_031902387.1 , XM_031902395.1 , XM_012966190.2
Accession Version	XM_012966190.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821644.1
CDS	330..3548
Translation	MFGATQVSKAQFLDKARQAREERKGLKERDRAAIKIQALIRRFLCRCRLQKEIRQEVDDFLEGTEKGSVKANALSIFRISRKLLIIFQISQDRMRFEKLCRCILNSMENDNEPKVWFVSLALSKDLTLLWIKQIKDILWYCSEYLKQLKPDILQDSKLITVYLTMLVTFTDTLTWKILKGKGESLRPAMNHICANIMGYLNQKGFYSVLQILLTNGLARSRPSLSKGTLTALFSLALRPVVAAQYSDNLLRSFLIHIMSVPALVSHLSTLTPDRLSVIESNGLFHKFILFLSREDQCSDVCECLEGSHTLCLLGNLIHLGHLTEKVLEEEMFHFVSVLTQMLSYCQKYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLLTKQLQHLWGVHMIRNLFREVLSKKLAENQDVSVPVSQSSSSSPQNNLPMKNLFKRAFQKSASVRHILKPIGGKKVDSPEVQKVCNICVLYQMALTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPQGGLKLFLECLSSDTEESRRLLAMLVLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGLLENAKGETLELFHSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPTLLFQELDKERKRAQLLLQYIPHVIPHKNRVLLFRNIVTKEKEKLGLVETNTASPHVTHITIRRSRMLEDGYDQLRQLSQNAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSSGDRLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSIFYSSVDELPSLDSEFYKNLTSIKRYDGDISDLGLTLSYDEDVMGQLVCHELVPGGKSIPVTNENKISYIHLMAHFRMHTQIKNQTVAFISGFRSIIRPEWIRMFSAPELQRLISGDNAEIDLDDLKKHTVYYGGFHGSHRVIIWLWDILANDFSSEEKAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKGILREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_012966190.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3B (ube3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966190.2

ORF Insert Sequence:

ATGTTTGGAG CAACTCAGGT TTCAAAGGCC CAGTTCTTAG ACAAAGCCAG GCAAGCTCGT 
GAAGAGAGAA AAGGTCTCAA AGAAAGAGAT AGAGCAGCAA TCAAAATTCA GGCACTTATC 
AGAAGGTTTT TGTGCAGATG TCGACTTCAG AAAGAAATCC GTCAAGAAGT CGATGATTTT 
CTAGAAGGCA CCGAAAAAGG TTCAGTCAAA GCCAATGCAC TTTCAATTTT CAGGATATCA 
CGCAAGCTGC TGATAATATT CCAGATTTCT CAGGACAGGA TGAGGTTTGA AAAGCTTTGC 
CGGTGTATAC TCAACAGCAT GGAAAATGAC AATGAACCCA AGGTTTGGTT TGTGTCTCTC 
GCTCTTTCTA AGGATCTCAC ACTTCTCTGG ATAAAGCAGA TAAAAGACAT TCTGTGGTAC 
TGTTCTGAAT ACCTTAAACA GTTAAAGCCA GATATCCTGC AGGACTCCAA GCTGATCACA 
GTCTACCTCA CTATGCTGGT CACCTTTACA GATACCCTAA CCTGGAAAAT ACTCAAGGGG 
AAAGGCGAAA GCCTGAGACC AGCTATGAAC CACATTTGTG CAAATATTAT GGGGTATCTT 
AACCAGAAAG GATTTTATTC TGTTCTACAG ATACTGTTAA CCAATGGCTT GGCCAGATCT 
CGCCCCTCCT TGTCCAAAGG CACGCTTACA GCCCTGTTTT CATTGGCGTT GCGTCCAGTA 
GTAGCAGCAC AATATTCTGA CAACCTTCTT CGATCCTTCT TGATCCACAT AATGTCTGTA 
CCAGCTTTGG TTTCCCACTT GTCTACACTT ACTCCAGACA GGCTTTCCGT AATAGAATCG 
AATGGTCTCT TCCATAAATT CATCTTGTTT CTGAGCCGGG AGGATCAGTG CAGTGACGTG 
TGTGAGTGCC TGGAGGGCAG CCATACTCTT TGTTTGCTAG GTAACCTCAT TCACCTGGGC 
CACCTGACAG AGAAGGTGTT GGAGGAGGAG ATGTTTCACT TTGTCAGTGT CCTTACCCAA 
ATGTTATCTT ATTGCCAGAA ATATGTGTCT CAGAAGAAGT CCAATCTTAC ACACTGGCAC 
CCTGTTCTGG GCTGGTTCTC CCAGACAGTG GACTATGGTC TCAATGAGTC CATGCCCTTA 
CTCACCAAGC AACTTCAACA TCTGTGGGGT GTCCATATGA TACGTAATTT GTTTCGTGAG 
GTCCTAAGCA AGAAGTTGGC AGAAAACCAA GACGTGTCTG TTCCTGTGTC ACAAAGCTCT 
TCTTCCTCCC CACAGAATAA TCTACCCATG AAGAATCTCT TTAAACGAGC GTTCCAGAAA 
TCTGCTTCTG TTCGACACAT CCTTAAACCT ATTGGGGGGA AAAAGGTAGA TTCTCCAGAA 
GTGCAAAAAG TTTGCAACAT CTGTGTTCTG TACCAGATGG CACTGACAAC TCTGACACAG 
ATACGGCTAC AGATCCTCAC AGGGCTTACC TATCTGGATG ACCTTTTGCC CAAGTTGTGG 
GCATTCATAT GTGAACTAGG ACCTCAGGGA GGTCTGAAAC TCTTCTTGGA ATGTCTGAGT 
AGTGACACAG AGGAGTCCCG TAGACTTCTG GCTATGCTGG TTTTGTTTTG CGACTGCTCT 
AGGCATTTAA TTACGATCTT GGATGATATT GAGGTATATG AAGAACAGAT ATCATTTAAA 
CTAGAGGAGC TTGTGACTAT TTCCTCCTTT CTGAACTCCT TTGTGTTCAA GATGATCTGG 
GATGGACTTC TTGAGAATGC CAAAGGAGAG ACTCTGGAGC TATTCCACTC TGTGCATGGA 
TGGCTCATGG TCCTGTATGA AAGAGACTGC CGTAGGCGTT TTGCCCCTGA GGATCATTGG 
CTGCGCAAGG ATCTCAAACC AACCCTGCTA TTCCAAGAAC TGGATAAAGA GAGAAAGAGA 
GCCCAGCTCT TACTTCAGTA TATCCCTCAT GTCATCCCAC ACAAAAACAG GGTCCTCTTG 
TTCCGAAATA TTGTCACCAA GGAAAAGGAG AAGTTGGGTT TAGTGGAAAC TAACACCGCA 
TCCCCACATG TCACCCACAT TACCATCCGA AGGTCACGTA TGTTAGAGGA TGGATACGAC 
CAACTAAGGC AGCTTTCCCA GAATGCCATG AAAGGTGTTA TACGTGTAAA GTTTGTGAAC 
GATCTTGGTG TAGATGAAGC TGGTATAGAC CAGGATGGTG TCTTTAAGGA GTTCTTAGAG 
GAAATTATCA AGAGAGTATT TGACCCTGCA CTGAATCTTT TTAAGACCAC TAGTTCTGGA 
GATAGGTTGT ACCCATCTCC TACATCTTAT ATACATGAAA ACTACCTCCA GCTGTTTGAA 
TTTGTAGGGA AGATGCTGGG CAAAGCAGTG TATGAGGGTA TAGTGGTGGA TGTCCCATTT 
GCATCATTCT TTCTCAGCCA GCTTCTTGGG CACCATCATA GCATCTTCTA CAGCTCTGTG 
GATGAGCTGC CTTCCTTGGA CTCTGAATTC TACAAGAATC TCACTTCCAT CAAGCGCTAC 
GATGGGGATA TTAGTGATTT GGGACTTACC CTTTCATACG ATGAGGATGT TATGGGTCAG 
CTGGTCTGTC ATGAACTGGT CCCAGGTGGG AAGTCTATTC CAGTGACTAA TGAAAATAAA 
ATCAGTTATA TCCACCTCAT GGCTCATTTC CGCATGCACA CGCAGATAAA GAACCAAACT 
GTTGCCTTCA TTAGTGGATT CCGCTCCATC ATAAGGCCTG AATGGATCCG CATGTTCTCA 
GCACCTGAAC TGCAACGTCT CATATCAGGG GACAATGCAG AGATTGACCT GGATGATCTA 
AAGAAACACA CAGTCTACTA TGGAGGGTTT CATGGAAGTC ATCGTGTCAT TATCTGGCTG 
TGGGATATTT TGGCCAATGA TTTCTCTTCT GAAGAAAAAG CCATGTTTCT TAAGTTTGTC 
ACCAGTTGCT CTCGGCCTCC TCTACTTGGA TTTGCCTACC TCAAACCTCC CTTTTCCATT 
AGATGTGTAG AGGTGTCAGA TGATCAGGAT ACAGGCGACA CCCTGGGCAG TGTCCTACGT 
GGGTTCTTCA CCATCCGAAA GAAAGAGCCT GGTGGACGCC TTCCCACTTC CTCCACCTGC 
TTCAATCTCC TGAAGCTTCC CAACTACAGT AAAAAAGGAA TTCTGAGGGA GAAACTACGC 
TATGCTATCA GCATGAACAC TGGTTTTGAG CTCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ube3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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