arhgap27 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgap27
Entrez Gene ID	779583
Full Name	Rho GTPase activating protein 27
Synonyms	camgap1,pp905,sh3d20,sh3p20
General protein information	Preferred Names Rho GTPase activating protein 27 Names rho GTPase-activating protein 27 Rho GTPase activating protein 15 SH3 domain containing 20
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933160.5	XP_002933206.3	rho GTPase-activating protein 27
XM_002933160.4	XP_002933206.2	rho GTPase-activating protein 27

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Arhgap27	NP_942054.1
Xenopus tropicalis (tropical clawed frog)	arhgap27	XP_002933206.2
Danio rerio (zebrafish)	LOC566523	XP_005171659.1
Homo sapiens (human)	ARHGAP27	NP_001269219.1
Pan troglodytes (chimpanzee)	ARHGAP27	XP_001139088.1
Mus musculus (house mouse)	Arhgap27	NP_001192165.1
Gallus gallus (chicken)	ARHGAP27	XP_003642832.2
Danio rerio (zebrafish)	arhgap27	XP_005156086.1

Gene Ontology

The following arhgap27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgap27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa50571	XM_002933160.5 Latest version!	Xenopus tropicalis Rho GTPase activating protein 27 (arhgap27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa17337	XM_002933160.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 27 (arhgap27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgap27 ( XM_002933160.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933206.3 Xenopus tropicalis Rho GTPase activating protein 27 (arhgap27), mRNA.

Price & Availability↑

CloneID	OXa50571
Clone ID Related Accession (Same CDS sequence)	XM_002933160.5
Accession Version	XM_002933160.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933160.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCAG CTGGATCTAA AGAACAGGAG CTGGTGTTGG TGGAACACGC CTTCGAGTAT 
TCATCTAAAG ATGGGAAAAT CATCTCTATA AAACCCAACG AGAGGTACAT CCTTCTGAAG 
AGGACTAATG AGCATTGGTG GCAGGTGTGC CTTGATAAGA CTACTACGCC ATTTTATATA 
CCGGCCAAAT ATGTCAAAGT GTTGCCCTTG GCTTCGAAAC GATTCAATGG TTATCAGCCC 
TACAAGGATC CATCAGAGGA TGTGCAGGTA GCTGTGCCTA GTAATGAGTA CAGCTACAAA 
TTCTTACCAC CATGCCCACA GGTGAGTGCC AATGTCACCC CACCGCCTTT GGATATCCAG 
CCGGCCATTA CTGATAATGC CATTACATCA AGAAATGAGT GTGCCAGCAA AGCCCTGGCA 
CCGGTTAAGG TGCCATGTAT CAATACAAGT AACACCCTAC CAAAAGTCAG CATGCCAAAG 
CTTGCTGCCA CCAACACCCT GCCATCTGCC ACAGTGCCTA GGGTGAACTC CAACAATGTC 
CAACCAAAAG TGGTACCACA GATCCATATA AATGATGTAT TAACCAGTAC CCCACAAATA 
CATTCAAGCA ATATCCTCCC AACCAATGAC AAGCAGAATA GTGCCACTCA GCCAGAACCA 
TCAACCCAAT TTCTGCCATC AGACCTCCCT ATGGTATGTG GAGCCTCAGG ACTGGACTCA 
AGTCACACAT ATCCAAAGGA ACAACCAGAG GACAGCCGCA CACATGCTCC TATGAGAACC 
TTCCACAATT CTAAGAAGGT TGTTGACCCC ACCAAGCGTA TCAGCTTCTT GTGTCTCACT 
GATGCCCAGC CCAATATTTT AACCACTCCT TCTTCAGGGA ATCTAAAACC AGAGCCATCT 
ACAACTTATC ACCAACAACA ACCACACAAA ATCAATGACT CTTCAGGATT GTGTTGCCCA 
GAACAGGTGG TGATAGTAAC AAGTAACACA GCTGGGTCCA ATGGAATCTC TCCTTTAGTC 
CAGCCAATTA AAGAAGAGGA AGACTGTGGG CCACTCTATG AAAACCTAAA GCCATTCCAA 
AAAGAGACCC CGAGGGAACG AGCCTCATCC CTGCAGCACA GTTCCACCAG CACCTTAGAC 
GACTGGGAAA CTCACACAGA CACAGGCAGC GGGCAACTAT TCTATTATAA CAGTGTTACA 
GGGGTAACAA CCTGGGATTC TCCGTTTGAC CAACCAGAAG ACCCAGAGCC CTCCCCCACC 
TCTCTGAATT CCCTAAGTCC TTTAGCTGAA GACTCGCAGT GGGAGAAACA TTTTGATGAA 
GCCACGAAAA AATACTATTT CTATAATTCA GTGACAGGGG AAACGTCCTG GGATCCACCA 
GTAGAAGAGA TGAGCTTTCA AGAATTTACT CCAATGGTAT CTTCATATAT ATCGGTGGAC 
CGGAGGCCAC CGACTCCAGA AGCTGATTAT CCTGACTATA CTCTAGAAGA GGGAGAGAGT 
TACCCCGATG CCGATTACCA TCAAGATCCC CCGGGCCTAT ATGAACAGAT TCCTTTACAC 
AAGTCTCCTG TTGATCCAAA TTCAGGCTGG TTCTGTCAGG TCAACAAGGA TGGGAAAAAG 
TTCTACTCAA ACAACTACAA CAGTGAAATT TGGGTACAGT CAGAGGACCA GTCAGGAAAA 
ACCTTTTTCT ACACCCAGGA TGGTTCCCTC TCACAGTGGA GTTTGCCACG GATCGGCCCA 
GCAAGTCTTC ATAGACCCAA TGATGACAAC AATGTCTTTA CAAAGTTGCA AGATAATTAC 
CCAGCAATCA CTGCACGGAA AGATACAGAG GATTTCGGCC ATAGGCTCAG GAGAGCTGGT 
TCTGAAGATT TCCTGAACGT GGGTGGGGAT CATCATCCTC ATCAGACCAA AAGCCTGGAA 
AAAGCTGGAG TCCTTCACAA GGCTAAAATA TCCGACAATG GTAAAAAGCT TCGTAAAAGC 
TGGAGCTCCT CCTGGACTGT CCTGGAAGGG GGAATTCTGA CATTCTTCAG GGATGGTAAA 
AATCTATCAT CTAACAGTTC GAGGCATTCC CAGCTATCTA CCCCAGAGTA CACAGTCAGG 
CTCGAAGGGG CCTCTCTCGT ATGGGCCACC AAGGATAAAT CAAGCAAGAA GCATGTACTA 
GAGCTGAAAA CGCGAAACGG CTCTGAGTAT CTCATACACC ATGATTCAGA GACGATCACT 
GCAGACTGGC ACGAATCAAT AAAGAACAGC ATTGCCAGGA ATATACGTGT ATCTGCAGAT 
CATACGCCTG AAGATGAGCC TGAACCATTC CAGGAATTTG GTTCTACTGA GAAGTTGGGC 
ACAAAAGATG AAAAAAAGAA CTCAAGCATG GGATCAGGCA GCAACTCTGA CAGCGATCGC 
AAAGTCCGCG CTAAGCTCAA GAAGTTTTTA CAAAGGCGGC CGACGATACA GTCCCTGCGA 
GACAGAGGTT ACATTAAAGA ACAAGTGTTT GGATGCCCCT TGCAACAGCT CTGTGAAAGA 
GAGAAACAGA ATGTTCCGGA CTTTGTAAGG AAGGCCATCC AAGCCGTGGA AAAAAGAGGA 
CTTGATATTG ATGGTTTATA TCGAGTAAGC GGAAATCTGG CCACTATACA GAAGCTACGC 
CACAAAGTGG ATCAAGAGGA AAACACCAAC CTGGAAGACG GGCGCTGGGA AGACGTCCAT 
GTTATCACCG GAGCCCTGAA GCTTTTCTTC CGGGAGCTGC CAGAACCTCT GTTCCCATTC 
AGTCACTTTG ACATGTTCAT TGAGACAATA AAATTGAATG ACCCGGCGCT GAAGAAGAAA 
CAGTTCAAGG AGCTGATACA GTCGCTGCCT CCGCCCAATC AGGAGACCAT GCAGTTTCTC 
TTCCGACATC TATGCAAAGT CATTGAATGT AAAGATTCCA ACAGAATGTC CATTCAGAGT 
GTCGCCATTG TGTTTGGGCC GACCCTCCTA AGGCCCTCGA TAGAAGGGGC CAACATCGCC 
ATGTACATGG TGTTTCAGAA CCAAATCGTG GAGCAAGTTC TAAGCCAATA CAAATACATA 
TTTAACACAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933206.3
CDS	416..3490
Translation	MQSAGSKEQELVLVEHAFEYSSKDGKIISIKPNERYILLKRTNEHWWQVCLDKTTTPFYIPAKYVKVLPLASKRFNGYQPYKDPSEDVQVAVPSNEYSYKFLPPCPQVSANVTPPPLDIQPAITDNAITSRNECASKALAPVKVPCINTSNTLPKVSMPKLAATNTLPSATVPRVNSNNVQPKVVPQIHINDVLTSTPQIHSSNILPTNDKQNSATQPEPSTQFLPSDLPMVCGASGLDSSHTYPKEQPEDSRTHAPMRTFHNSKKVVDPTKRISFLCLTDAQPNILTTPSSGNLKPEPSTTYHQQQPHKINDSSGLCCPEQVVIVTSNTAGSNGISPLVQPIKEEEDCGPLYENLKPFQKETPRERASSLQHSSTSTLDDWETHTDTGSGQLFYYNSVTGVTTWDSPFDQPEDPEPSPTSLNSLSPLAEDSQWEKHFDEATKKYYFYNSVTGETSWDPPVEEMSFQEFTPMVSSYISVDRRPPTPEADYPDYTLEEGESYPDADYHQDPPGLYEQIPLHKSPVDPNSGWFCQVNKDGKKFYSNNYNSEIWVQSEDQSGKTFFYTQDGSLSQWSLPRIGPASLHRPNDDNNVFTKLQDNYPAITARKDTEDFGHRLRRAGSEDFLNVGGDHHPHQTKSLEKAGVLHKAKISDNGKKLRKSWSSSWTVLEGGILTFFRDGKNLSSNSSRHSQLSTPEYTVRLEGASLVWATKDKSSKKHVLELKTRNGSEYLIHHDSETITADWHESIKNSIARNIRVSADHTPEDEPEPFQEFGSTEKLGTKDEKKNSSMGSGSNSDSDRKVRAKLKKFLQRRPTIQSLRDRGYIKEQVFGCPLQQLCEREKQNVPDFVRKAIQAVEKRGLDIDGLYRVSGNLATIQKLRHKVDQEENTNLEDGRWEDVHVITGALKLFFRELPEPLFPFSHFDMFIETIKLNDPALKKKQFKELIQSLPPPNQETMQFLFRHLCKVIECKDSNRMSIQSVAIVFGPTLLRPSIEGANIAMYMVFQNQIVEQVLSQYKYIFNTS

Target ORF information:

RefSeq Version	XM_002933160.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 27 (arhgap27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933160.5

ORF Insert Sequence:

ATGCAGTCAG CTGGATCTAA AGAACAGGAG CTGGTGTTGG TGGAACACGC CTTCGAGTAT 
TCATCTAAAG ATGGGAAAAT CATCTCTATA AAACCCAACG AGAGGTACAT CCTTCTGAAG 
AGGACTAATG AGCATTGGTG GCAGGTGTGC CTTGATAAGA CTACTACGCC ATTTTATATA 
CCGGCCAAAT ATGTCAAAGT GTTGCCCTTG GCTTCGAAAC GATTCAATGG TTATCAGCCC 
TACAAGGATC CATCAGAGGA TGTGCAGGTA GCTGTGCCTA GTAATGAGTA CAGCTACAAA 
TTCTTACCAC CATGCCCACA GGTGAGTGCC AATGTCACCC CACCGCCTTT GGATATCCAG 
CCGGCCATTA CTGATAATGC CATTACATCA AGAAATGAGT GTGCCAGCAA AGCCCTGGCA 
CCGGTTAAGG TGCCATGTAT CAATACAAGT AACACCCTAC CAAAAGTCAG CATGCCAAAG 
CTTGCTGCCA CCAACACCCT GCCATCTGCC ACAGTGCCTA GGGTGAACTC CAACAATGTC 
CAACCAAAAG TGGTACCACA GATCCATATA AATGATGTAT TAACCAGTAC CCCACAAATA 
CATTCAAGCA ATATCCTCCC AACCAATGAC AAGCAGAATA GTGCCACTCA GCCAGAACCA 
TCAACCCAAT TTCTGCCATC AGACCTCCCT ATGGTATGTG GAGCCTCAGG ACTGGACTCA 
AGTCACACAT ATCCAAAGGA ACAACCAGAG GACAGCCGCA CACATGCTCC TATGAGAACC 
TTCCACAATT CTAAGAAGGT TGTTGACCCC ACCAAGCGTA TCAGCTTCTT GTGTCTCACT 
GATGCCCAGC CCAATATTTT AACCACTCCT TCTTCAGGGA ATCTAAAACC AGAGCCATCT 
ACAACTTATC ACCAACAACA ACCACACAAA ATCAATGACT CTTCAGGATT GTGTTGCCCA 
GAACAGGTGG TGATAGTAAC AAGTAACACA GCTGGGTCCA ATGGAATCTC TCCTTTAGTC 
CAGCCAATTA AAGAAGAGGA AGACTGTGGG CCACTCTATG AAAACCTAAA GCCATTCCAA 
AAAGAGACCC CGAGGGAACG AGCCTCATCC CTGCAGCACA GTTCCACCAG CACCTTAGAC 
GACTGGGAAA CTCACACAGA CACAGGCAGC GGGCAACTAT TCTATTATAA CAGTGTTACA 
GGGGTAACAA CCTGGGATTC TCCGTTTGAC CAACCAGAAG ACCCAGAGCC CTCCCCCACC 
TCTCTGAATT CCCTAAGTCC TTTAGCTGAA GACTCGCAGT GGGAGAAACA TTTTGATGAA 
GCCACGAAAA AATACTATTT CTATAATTCA GTGACAGGGG AAACGTCCTG GGATCCACCA 
GTAGAAGAGA TGAGCTTTCA AGAATTTACT CCAATGGTAT CTTCATATAT ATCGGTGGAC 
CGGAGGCCAC CGACTCCAGA AGCTGATTAT CCTGACTATA CTCTAGAAGA GGGAGAGAGT 
TACCCCGATG CCGATTACCA TCAAGATCCC CCGGGCCTAT ATGAACAGAT TCCTTTACAC 
AAGTCTCCTG TTGATCCAAA TTCAGGCTGG TTCTGTCAGG TCAACAAGGA TGGGAAAAAG 
TTCTACTCAA ACAACTACAA CAGTGAAATT TGGGTACAGT CAGAGGACCA GTCAGGAAAA 
ACCTTTTTCT ACACCCAGGA TGGTTCCCTC TCACAGTGGA GTTTGCCACG GATCGGCCCA 
GCAAGTCTTC ATAGACCCAA TGATGACAAC AATGTCTTTA CAAAGTTGCA AGATAATTAC 
CCAGCAATCA CTGCACGGAA AGATACAGAG GATTTCGGCC ATAGGCTCAG GAGAGCTGGT 
TCTGAAGATT TCCTGAACGT GGGTGGGGAT CATCATCCTC ATCAGACCAA AAGCCTGGAA 
AAAGCTGGAG TCCTTCACAA GGCTAAAATA TCCGACAATG GTAAAAAGCT TCGTAAAAGC 
TGGAGCTCCT CCTGGACTGT CCTGGAAGGG GGAATTCTGA CATTCTTCAG GGATGGTAAA 
AATCTATCAT CTAACAGTTC GAGGCATTCC CAGCTATCTA CCCCAGAGTA CACAGTCAGG 
CTCGAAGGGG CCTCTCTCGT ATGGGCCACC AAGGATAAAT CAAGCAAGAA GCATGTACTA 
GAGCTGAAAA CGCGAAACGG CTCTGAGTAT CTCATACACC ATGATTCAGA GACGATCACT 
GCAGACTGGC ACGAATCAAT AAAGAACAGC ATTGCCAGGA ATATACGTGT ATCTGCAGAT 
CATACGCCTG AAGATGAGCC TGAACCATTC CAGGAATTTG GTTCTACTGA GAAGTTGGGC 
ACAAAAGATG AAAAAAAGAA CTCAAGCATG GGATCAGGCA GCAACTCTGA CAGCGATCGC 
AAAGTCCGCG CTAAGCTCAA GAAGTTTTTA CAAAGGCGGC CGACGATACA GTCCCTGCGA 
GACAGAGGTT ACATTAAAGA ACAAGTGTTT GGATGCCCCT TGCAACAGCT CTGTGAAAGA 
GAGAAACAGA ATGTTCCGGA CTTTGTAAGG AAGGCCATCC AAGCCGTGGA AAAAAGAGGA 
CTTGATATTG ATGGTTTATA TCGAGTAAGC GGAAATCTGG CCACTATACA GAAGCTACGC 
CACAAAGTGG ATCAAGAGGA AAACACCAAC CTGGAAGACG GGCGCTGGGA AGACGTCCAT 
GTTATCACCG GAGCCCTGAA GCTTTTCTTC CGGGAGCTGC CAGAACCTCT GTTCCCATTC 
AGTCACTTTG ACATGTTCAT TGAGACAATA AAATTGAATG ACCCGGCGCT GAAGAAGAAA 
CAGTTCAAGG AGCTGATACA GTCGCTGCCT CCGCCCAATC AGGAGACCAT GCAGTTTCTC 
TTCCGACATC TATGCAAAGT CATTGAATGT AAAGATTCCA ACAGAATGTC CATTCAGAGT 
GTCGCCATTG TGTTTGGGCC GACCCTCCTA AGGCCCTCGA TAGAAGGGGC CAACATCGCC 
ATGTACATGG TGTTTCAGAA CCAAATCGTG GAGCAAGTTC TAAGCCAATA CAAATACATA 
TTTAACACAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap27 ( XM_002933160.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933206.2 Xenopus tropicalis Rho GTPase activating protein 27 (arhgap27), mRNA.

Price & Availability↑

CloneID	OXa17337
Clone ID Related Accession (Same CDS sequence)	XM_002933160.4
Accession Version	XM_002933160.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933160.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCAG CTGGATCTAA AGAACAGGAG CTGGTGTTGG TGGAACACGC CTTCGAGTAT 
TCATCTAAAG ATGGGAAAAT CATCTCTATA AAACCCAACG AGAGGTACAT CCTTCTGAAG 
AGGACTAATG AGCATTGGTG GCAGGTGTGC CTTGATAAGA CTACTACGCC ATTTTATATA 
CCGGCCAAAT ATGTCAAAGT GTTGCCCTTG GCTTCGAAAC GATTCAATGG TTATCAGCCC 
TACAAGGATC CATCAGAGGA TGTGCAGGTA GCTGTGCCTA GTAATGAGTA CAGCTACAAA 
TTCTTACCAC CATGCCCACA GGTGAGTGCC AATGTCACCC CACCGCCTTT GGATATCCAG 
CCGGCCATTA CTGATAATGC CATTACATCA AGAAATGAGT GTGCCAGCAA AGCCCTGGCA 
CCGGTTAAGG TGCCATGTAT CAATACAAGT AACACCCTAC CAAAAGTCAG CATGCCAAAG 
CTTGCTGCCA CCAACACCCT GCCATCTGCC ACAGTGCCTA GGGTGAACTC CAACAATGTC 
CAACCAAAAG TGGTACCACA GATCCATATA AATGATGTAT TAACCAGTAC CCCACAAATA 
CATTCAAGCA ATATCCTCCC AACCAATGAC AAGCAGAATA GTGCCACTCA GCCAGAACCA 
TCAACCCAAT TTCTGCCATC AGACCTCCCT ATGGTATGTG GAGCCTCAGG ACTGGACTCA 
AGTCACACAT ATCCAAAGGA ACAACCAGAG GACAGCCGCA CACATGCTCC TATGAGAACC 
TTCCACAATT CTAAGAAGGT TGTTGACCCC ACCAAGCGTA TCAGCTTCTT GTGTCTCACT 
GATGCCCAGC CCAATATTTT AACCACTCCT TCTTCAGGGA ATCTAAAACC AGAGCCATCT 
ACAATTTATC ACCAACAACA ACCACACAAA ATCAATGACT CTTCGGGATT GTGTTGCCCA 
GAACAGGTGG TGATAGTAAC AAGTAACACA GCTGGGTCCA ATGGAGTCTC TCCTTTAGTC 
CAGCCAATTA AAGAAGAGGA AGACTGTGGG CCACTCTATG AAAACCTAAA GCCATTCCAA 
AAAGAGACCC CGAGGGAACG AGCCTCATCC CTGCAGCACA GTTCCACCAG CACCTTAGAC 
GACTGGGAAA CTCACACAGA CACAGGCAGC GGGCAACTAT TCTATTATAA CAGTGTTACA 
GGGGTAACAA CCTGGGATTC TCCGTTTGAC CAACCAGAAG ACCCAGAGCC CTCCCCCACC 
TCTCTGAATT CCCTAAGTCC TTTAGCTGAA GACTCGCAGT GGGAGAAACA TTTTGATGCA 
GCCACGAAAA AATACTATTT CTATAATTCA GTGACAGGGG AAACGTCCTG GGATCCACCA 
GTAGAAGAGA TGAGCTTTCA AGAATTTACT CCAATGGTAT CTTCATATAT ATCGGTGGAC 
CGGAGGCCAC CGACTCCAGA AGCTGATTAT CCTGGCTATA CTCTAGAAGA GGGAGAGAGT 
TACCCCGATG CCGATTACCA TCAAGATCCC CCGGGCCTAT ATGAACAGAT TCCTTTACAC 
AAGTCTCCTG TTGATCCAAA TTCAGGCTGG TTCTGTCAGG TCAACAAGGA TGGGAAAAAG 
TTCTACTCAA ACAACTACAA CAGTGAAATT TGGGTACAGT CAGAGGACCA GTCAGGAAAA 
ACCTTTTTCT ACACCCAGGA TGGTTCCCTC TCACAGTGGA GTTTGCCACG GATCGGCCCA 
GCAAGTCTTC ATAGACCCAA TGATGACAAC AATGTCTTTA CAAAGTTGCA AGATAATTAC 
CCAGCAATCA CTGCACGGAA AGATACAGAG GATTTCGGCC ATAGGCTCAG GAGAGCTGGT 
TCTGAAGATT TCCTGAACGT GGGTGGGGAT CATCATCATC ATCAGACCAA AAGCCTGGAA 
AAAGCTGGAG TCCTTCACAA GGCTAAAATA TCCGACAATG GTAAAAAGCT TCGTAAAAGC 
TGGAGCTCCT CCTGGACTGT CCTGGAAGGG GGAATTCTGA CATTCTTCAG GGATGGTAAA 
AATCTATCAT CTAACAGTTC GAGGCATTCC CAGCTATCTA CCCCAGAGTA CACAGTCAGG 
CTCGAAGGGG CCTCTCTCGT ATGGGCCACC AAGGATAAAT CAAGCAAGAA GCATGTACTA 
GAGCTGAAAA CTCGAAACGG CTCTGAGTAT CTCATACACC ATGATTCAGA GACGATCACT 
GCAGACTGGC ACGAATCAAT AAAGAACAGC ATTGCCAGGA ATATACGTGT ATCTGCAGAT 
CATACGCCTG AAGATGAGCC TGAACCATTC CAGGAATTTG GTTCTACTGA GAAGTTGGGC 
ACAAAAGATG AAAAAAAGAA CTCAAGCATG GGATCAGGCA GCAACTCTGA CAGCGATCGC 
AAAGTCCGCG CTAAGCTCAA GAAGTTTTTA CAAAGGCGGC CGACGATACA GTCCCTGCGA 
GACAGAGGTT ACATTAAAGA ACAAGTGTTT GGATGCCCCT TGCAACAGCT CTGTGAAAGA 
GAGAAACAGA ATGTTCCGGA CTTTGTAAGG AAGGCCATCC AAGCCGTGGA AAAAAGAGGA 
CTTGATATTG ATGGTTTATA TCGAGTGAGC GGAAATCTGG CCACTATACA GAAACTACGC 
CACAAAGTGG ATCAAGAGGA AAACACCAAC CTGGAAGACG GGCGCTGGGA AGACGTCCAT 
GTTATCACCG GAGCCCTGAA GCTTTTCTTC CGGGAGCTGC CAGAACCTCT GTTCCCATTC 
AGTCACTTTG ACATGTTCAT TGAGACAATA AAATTGAATG ACCCGGCGCT GAAGAAGAAA 
CAGTTCAAGG AGCTGATACA GTCGCTGCCT CCGCCCAATC AGGAGACCAT GCAGTTTCTC 
TTCCGACATC TATGCAAAGT CATTGAATGT AAAGATTCCA ACAGAATGTC CATTCAGAGT 
GTCGCCATTG TGTTTGGGCC GACCCTCCTA AGGCCCTCGA TAGAAGGGGC CAACATCGCC 
ATGTACATGG TGTTTCAGAA CCAAATCGTG GAGCAAGTTC TAAGCCAATA CAAATACATA 
TTTAACACAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933206.2
CDS	431..3505
Translation	MQSAGSKEQELVLVEHAFEYSSKDGKIISIKPNERYILLKRTNEHWWQVCLDKTTTPFYIPAKYVKVLPLASKRFNGYQPYKDPSEDVQVAVPSNEYSYKFLPPCPQVSANVTPPPLDIQPAITDNAITSRNECASKALAPVKVPCINTSNTLPKVSMPKLAATNTLPSATVPRVNSNNVQPKVVPQIHINDVLTSTPQIHSSNILPTNDKQNSATQPEPSTQFLPSDLPMVCGASGLDSSHTYPKEQPEDSRTHAPMRTFHNSKKVVDPTKRISFLCLTDAQPNILTTPSSGNLKPEPSTIYHQQQPHKINDSSGLCCPEQVVIVTSNTAGSNGVSPLVQPIKEEEDCGPLYENLKPFQKETPRERASSLQHSSTSTLDDWETHTDTGSGQLFYYNSVTGVTTWDSPFDQPEDPEPSPTSLNSLSPLAEDSQWEKHFDAATKKYYFYNSVTGETSWDPPVEEMSFQEFTPMVSSYISVDRRPPTPEADYPGYTLEEGESYPDADYHQDPPGLYEQIPLHKSPVDPNSGWFCQVNKDGKKFYSNNYNSEIWVQSEDQSGKTFFYTQDGSLSQWSLPRIGPASLHRPNDDNNVFTKLQDNYPAITARKDTEDFGHRLRRAGSEDFLNVGGDHHHHQTKSLEKAGVLHKAKISDNGKKLRKSWSSSWTVLEGGILTFFRDGKNLSSNSSRHSQLSTPEYTVRLEGASLVWATKDKSSKKHVLELKTRNGSEYLIHHDSETITADWHESIKNSIARNIRVSADHTPEDEPEPFQEFGSTEKLGTKDEKKNSSMGSGSNSDSDRKVRAKLKKFLQRRPTIQSLRDRGYIKEQVFGCPLQQLCEREKQNVPDFVRKAIQAVEKRGLDIDGLYRVSGNLATIQKLRHKVDQEENTNLEDGRWEDVHVITGALKLFFRELPEPLFPFSHFDMFIETIKLNDPALKKKQFKELIQSLPPPNQETMQFLFRHLCKVIECKDSNRMSIQSVAIVFGPTLLRPSIEGANIAMYMVFQNQIVEQVLSQYKYIFNTS

Target ORF information:

RefSeq Version	XM_002933160.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 27 (arhgap27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933160.4

ORF Insert Sequence:

ATGCAGTCAG CTGGATCTAA AGAACAGGAG CTGGTGTTGG TGGAACACGC CTTCGAGTAT 
TCATCTAAAG ATGGGAAAAT CATCTCTATA AAACCCAACG AGAGGTACAT CCTTCTGAAG 
AGGACTAATG AGCATTGGTG GCAGGTGTGC CTTGATAAGA CTACTACGCC ATTTTATATA 
CCGGCCAAAT ATGTCAAAGT GTTGCCCTTG GCTTCGAAAC GATTCAATGG TTATCAGCCC 
TACAAGGATC CATCAGAGGA TGTGCAGGTA GCTGTGCCTA GTAATGAGTA CAGCTACAAA 
TTCTTACCAC CATGCCCACA GGTGAGTGCC AATGTCACCC CACCGCCTTT GGATATCCAG 
CCGGCCATTA CTGATAATGC CATTACATCA AGAAATGAGT GTGCCAGCAA AGCCCTGGCA 
CCGGTTAAGG TGCCATGTAT CAATACAAGT AACACCCTAC CAAAAGTCAG CATGCCAAAG 
CTTGCTGCCA CCAACACCCT GCCATCTGCC ACAGTGCCTA GGGTGAACTC CAACAATGTC 
CAACCAAAAG TGGTACCACA GATCCATATA AATGATGTAT TAACCAGTAC CCCACAAATA 
CATTCAAGCA ATATCCTCCC AACCAATGAC AAGCAGAATA GTGCCACTCA GCCAGAACCA 
TCAACCCAAT TTCTGCCATC AGACCTCCCT ATGGTATGTG GAGCCTCAGG ACTGGACTCA 
AGTCACACAT ATCCAAAGGA ACAACCAGAG GACAGCCGCA CACATGCTCC TATGAGAACC 
TTCCACAATT CTAAGAAGGT TGTTGACCCC ACCAAGCGTA TCAGCTTCTT GTGTCTCACT 
GATGCCCAGC CCAATATTTT AACCACTCCT TCTTCAGGGA ATCTAAAACC AGAGCCATCT 
ACAATTTATC ACCAACAACA ACCACACAAA ATCAATGACT CTTCGGGATT GTGTTGCCCA 
GAACAGGTGG TGATAGTAAC AAGTAACACA GCTGGGTCCA ATGGAGTCTC TCCTTTAGTC 
CAGCCAATTA AAGAAGAGGA AGACTGTGGG CCACTCTATG AAAACCTAAA GCCATTCCAA 
AAAGAGACCC CGAGGGAACG AGCCTCATCC CTGCAGCACA GTTCCACCAG CACCTTAGAC 
GACTGGGAAA CTCACACAGA CACAGGCAGC GGGCAACTAT TCTATTATAA CAGTGTTACA 
GGGGTAACAA CCTGGGATTC TCCGTTTGAC CAACCAGAAG ACCCAGAGCC CTCCCCCACC 
TCTCTGAATT CCCTAAGTCC TTTAGCTGAA GACTCGCAGT GGGAGAAACA TTTTGATGCA 
GCCACGAAAA AATACTATTT CTATAATTCA GTGACAGGGG AAACGTCCTG GGATCCACCA 
GTAGAAGAGA TGAGCTTTCA AGAATTTACT CCAATGGTAT CTTCATATAT ATCGGTGGAC 
CGGAGGCCAC CGACTCCAGA AGCTGATTAT CCTGGCTATA CTCTAGAAGA GGGAGAGAGT 
TACCCCGATG CCGATTACCA TCAAGATCCC CCGGGCCTAT ATGAACAGAT TCCTTTACAC 
AAGTCTCCTG TTGATCCAAA TTCAGGCTGG TTCTGTCAGG TCAACAAGGA TGGGAAAAAG 
TTCTACTCAA ACAACTACAA CAGTGAAATT TGGGTACAGT CAGAGGACCA GTCAGGAAAA 
ACCTTTTTCT ACACCCAGGA TGGTTCCCTC TCACAGTGGA GTTTGCCACG GATCGGCCCA 
GCAAGTCTTC ATAGACCCAA TGATGACAAC AATGTCTTTA CAAAGTTGCA AGATAATTAC 
CCAGCAATCA CTGCACGGAA AGATACAGAG GATTTCGGCC ATAGGCTCAG GAGAGCTGGT 
TCTGAAGATT TCCTGAACGT GGGTGGGGAT CATCATCATC ATCAGACCAA AAGCCTGGAA 
AAAGCTGGAG TCCTTCACAA GGCTAAAATA TCCGACAATG GTAAAAAGCT TCGTAAAAGC 
TGGAGCTCCT CCTGGACTGT CCTGGAAGGG GGAATTCTGA CATTCTTCAG GGATGGTAAA 
AATCTATCAT CTAACAGTTC GAGGCATTCC CAGCTATCTA CCCCAGAGTA CACAGTCAGG 
CTCGAAGGGG CCTCTCTCGT ATGGGCCACC AAGGATAAAT CAAGCAAGAA GCATGTACTA 
GAGCTGAAAA CTCGAAACGG CTCTGAGTAT CTCATACACC ATGATTCAGA GACGATCACT 
GCAGACTGGC ACGAATCAAT AAAGAACAGC ATTGCCAGGA ATATACGTGT ATCTGCAGAT 
CATACGCCTG AAGATGAGCC TGAACCATTC CAGGAATTTG GTTCTACTGA GAAGTTGGGC 
ACAAAAGATG AAAAAAAGAA CTCAAGCATG GGATCAGGCA GCAACTCTGA CAGCGATCGC 
AAAGTCCGCG CTAAGCTCAA GAAGTTTTTA CAAAGGCGGC CGACGATACA GTCCCTGCGA 
GACAGAGGTT ACATTAAAGA ACAAGTGTTT GGATGCCCCT TGCAACAGCT CTGTGAAAGA 
GAGAAACAGA ATGTTCCGGA CTTTGTAAGG AAGGCCATCC AAGCCGTGGA AAAAAGAGGA 
CTTGATATTG ATGGTTTATA TCGAGTGAGC GGAAATCTGG CCACTATACA GAAACTACGC 
CACAAAGTGG ATCAAGAGGA AAACACCAAC CTGGAAGACG GGCGCTGGGA AGACGTCCAT 
GTTATCACCG GAGCCCTGAA GCTTTTCTTC CGGGAGCTGC CAGAACCTCT GTTCCCATTC 
AGTCACTTTG ACATGTTCAT TGAGACAATA AAATTGAATG ACCCGGCGCT GAAGAAGAAA 
CAGTTCAAGG AGCTGATACA GTCGCTGCCT CCGCCCAATC AGGAGACCAT GCAGTTTCTC 
TTCCGACATC TATGCAAAGT CATTGAATGT AAAGATTCCA ACAGAATGTC CATTCAGAGT 
GTCGCCATTG TGTTTGGGCC GACCCTCCTA AGGCCCTCGA TAGAAGGGGC CAACATCGCC 
ATGTACATGG TGTTTCAGAA CCAAATCGTG GAGCAAGTTC TAAGCCAATA CAAATACATA 
TTTAACACAA GCTGA