gtf2i cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gtf2i
Entrez Gene ID	549986
Full Name	general transcription factor IIi
Synonyms	bap-135,bap135,btkap1,diws,gtfii-i,ib291,spin,tfii-i,wbs,wbscr6
General protein information	Preferred Names general transcription factor IIi Names general transcription factor II-I general transcription factor IIi
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031896978.1	XP_031752838.1	general transcription factor II-I isoform X3
XM_012957546.2	XP_012813000.1	general transcription factor II-I
XM_031896976.1	XP_031752836.1	general transcription factor II-I isoform X1
XM_031896977.1	XP_031752837.1	general transcription factor II-I isoform X2
XM_031896975.1	XP_031752835.1	general transcription factor II-I isoform X1
XM_031896979.1	XP_031752839.1	general transcription factor II-I isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GTF2I	XP_001151627.2
Bos taurus (cattle)	GTF2I	NP_001095647.1
	GTF2I	XP_001083724.2
Rattus norvegicus (Norway rat)	Gtf2i	NP_001001512.2
Gallus gallus (chicken)	GTF2I	XP_003642440.2
Homo sapiens (human)	GTF2I	NP_127492.1
Xenopus tropicalis (tropical clawed frog)	gtf2i	XP_002932326.2
Canis lupus familiaris (dog)	GTF2I	XP_005621002.1
Mus musculus (house mouse)	Gtf2i	NP_001074215.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gtf2i gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gtf2i cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa28123	XM_012957546.2 Latest version!	Xenopus tropicalis general transcription factor IIi (gtf2i), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa28123	XM_031896976.1 Latest version!	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa28123	XM_031896975.1 Latest version!	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa52416	XM_031896977.1 Latest version!	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa52417	XM_031896978.1 Latest version!	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa52418	XM_031896979.1 Latest version!	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gtf2i ( XM_012957546.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813000.1 Xenopus tropicalis general transcription factor IIi (gtf2i), mRNA.

Price & Availability↑

CloneID	OXa28123
Clone ID Related Accession (Same CDS sequence)	XM_012957546.2 , XM_031896976.1 , XM_031896975.1
Accession Version	XM_012957546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	general transcription factor II-I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) and transcript sequence (BC168446.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 54 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCTG AGGCCTTAGG CAAAACAGAG GCTATGTCTG TGCCATACCA GAAATTTGAA 
GCAAACCCTG CTGAAATGTA TGTGGAAGGG TTGCCTGATA ATATTCCATT CCGTAGCCCT 
TCCTGGTATG GAATTCCAAG ACTGGAAAAG ATAATTCAGG TTGGCAACAG GATCAAATTT 
GTGATTAAAA AACCAGAATT GCTGACTCAG TCTGTAGCTG AATCAATGCA AAACAGAACA 
AATTCAGGAG TGAAAGAAGA CTGGAATGTG AGGATTACTA AATTAAGAAA GCAGGTAGAG 
GAGATCTTCA ATACAAAATT TGCACAAGCA CTGGGACTTC CTGAAGCAGT AAAAGTCCCT 
TATCCAGTAT TTGAGTCTAA CCCAGAGTTC CTGTATGTCG AAGGATTGCC TGAAGGAATT 
CCCTTCCGGA GCCCCACATG GTTTGGGATA CCTCGGCTAG AGAGAATTGT TCGAGGAAGT 
GCGAAAATTA AATTTATAGT TAAGAAACCT CAGCTGGTTG CAACACACAT TCCTTCTGAA 
TTGGTCAACA AAATAAATAT TGATGTGTCA CAATCTACAG AGAGCGGAAG CAATCCAGCA 
AGTAAGCCAC CCGAGGCAGC AGTTGATGTT GATACCTCAA AATCATGCAT ACGGACGACT 
CCTGCCCAGA CTAATGGTTC CAGTATGCCT TTCAAACCCC GGGGAAGAGA ATTTTCTTTT 
GAGGCGTGGA ATGCCAAAAT TACAGACTTG AAACAAAAAG TAGAAGCCCT GTTTGATGAG 
AAATGTGGGG AGGCACTTGG TCTTAATGAA CCCTATAAGG TTCCTTTTGC ATTGTTTGAA 
TCATACCCAG AAGATTTCTA CGTGGAAGGG CTTCCTGAGG GTGTTCCCTT CCGCCGGCCG 
TCCACATTTG GTATCCCTAG ACTTGAGAAG ATTCTCAGAA ACAGGGCAAA AATTAAATTT 
GTTATTAAAA AACCTGAAAT GATTGAAAAC ACTGCCAAAG AACAGCCTCC AAACTTTGCA 
CCAAAAAAGG CAGGAATTCA GAACAATGCA CATACAGCTT CTGGACTTGA AGATCTGAAT 
ATAATCCAAG TGACAATACC AGATGCAGAC AATGAAAAAA ACGTTAAAGT AGACAAAGCC 
CGACAGCTGA GGGAGCAAGT CAATGAACTG TTCAGTAAGA AGTTCGGAGA AGCCATTGGC 
ATGAACTTTC CAGTAAAAGT CCCTTACAGG AAAATTACAA CTAACCCAGG ATGTGTGGTA 
GTTGATGGAA TGCCTCCTGG AGTTTCATTC AAAGCCCCCA GTTACCTTGA AATGAGTTCT 
ATGCGTAGGA TTCTGGATTC AGCTGAATTT ATTAAGTTTA CAGTTATAAG GCCTTTTCCA 
GGGCTGGTTC TTAACAACCA AGAAAGTGAA TCCGGACAGT CAGCAGAAAA TGCTGACAGA 
AGTCAAACAG ATGAAGCTGC AGAAGAAGGA TCAAAAGACA CACAAGACTT AAGTCTTATA 
AAACAAGAAC CAGATCCCAC GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813000.1
CDS	226..3072
Translation	MARFQMPDLPVLNESSDHREAIKHFITAVESMCTEFAKIKAEVACIAVYDHETLIAGSEKGRSFLDARKDFQKAFIQYCKLEEENTPQHHVIISSKAATDCAEIESLQKSVEDLFCICYGKALGKSTPVPVPYDKMVRDPTAVSVTGLPKDVFVKDPSNYDLETVTQILHNRSQVSFSIKRPFLDRRKSDEDAVTDYNDASVSESYNTVQVKTEPSEDSESSLELTTVTIKEEIDDQDYYQFNFQGCGYGEESNEATEVEQREDSALQVPSETSEDPEVEVTIEDDEYIPPNKKPKSTEIVNEPLQFKRKTREFNFEKWNARITELRKQVEELFEKKYAQAINVTGPVAIPYHLFQSNTEDLYVEGLPEGIPFRRPSTYGIPRLERILLARERIRFVIKKHDLLNARDVMPLEKPNTGVKEEWYARITKLRKIVDQLFSKKFAEALGKTEAMSVPYQKFEANPAEMYVEGLPDNIPFRSPSWYGIPRLEKIIQVGNRIKFVIKKPELLTQSVAESMQNRTNSGVKEDWNVRITKLRKQVEEIFNTKFAQALGLPEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSAKIKFIVKKPQLVATHIPSELVNKINIDVSQSTESGSNPASKPPEAAVDVDTSKSCIRTTPAQTNGSSMPFKPRGREFSFEAWNAKITDLKQKVEALFDEKCGEALGLNEPYKVPFALFESYPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNRAKIKFVIKKPEMIENTAKEQPPNFAPKKAGIQNNAHTASGLEDLNIIQVTIPDADNEKNVKVDKARQLREQVNELFSKKFGEAIGMNFPVKVPYRKITTNPGCVVVDGMPPGVSFKAPSYLEMSSMRRILDSAEFIKFTVIRPFPGLVLNNQESESGQSAENADRSQTDEAAEEGSKDTQDLSLIKQEPDPTW

Target ORF information:

RefSeq Version	XM_012957546.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis general transcription factor IIi (gtf2i), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957546.2

ORF Insert Sequence:

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCTG AGGCCTTAGG CAAAACAGAG GCTATGTCTG TGCCATACCA GAAATTTGAA 
GCAAACCCTG CTGAAATGTA TGTGGAAGGG TTGCCTGATA ATATTCCATT CCGTAGCCCT 
TCCTGGTATG GAATTCCAAG ACTGGAAAAG ATAATTCAGG TTGGCAACAG GATCAAATTT 
GTGATTAAAA AACCAGAATT GCTGACTCAG TCTGTAGCTG AATCAATGCA AAACAGAACA 
AATTCAGGAG TGAAAGAAGA CTGGAATGTG AGGATTACTA AATTAAGAAA GCAGGTAGAG 
GAGATCTTCA ATACAAAATT TGCACAAGCA CTGGGACTTC CTGAAGCAGT AAAAGTCCCT 
TATCCAGTAT TTGAGTCTAA CCCAGAGTTC CTGTATGTCG AAGGATTGCC TGAAGGAATT 
CCCTTCCGGA GCCCCACATG GTTTGGGATA CCTCGGCTAG AGAGAATTGT TCGAGGAAGT 
GCGAAAATTA AATTTATAGT TAAGAAACCT CAGCTGGTTG CAACACACAT TCCTTCTGAA 
TTGGTCAACA AAATAAATAT TGATGTGTCA CAATCTACAG AGAGCGGAAG CAATCCAGCA 
AGTAAGCCAC CCGAGGCAGC AGTTGATGTT GATACCTCAA AATCATGCAT ACGGACGACT 
CCTGCCCAGA CTAATGGTTC CAGTATGCCT TTCAAACCCC GGGGAAGAGA ATTTTCTTTT 
GAGGCGTGGA ATGCCAAAAT TACAGACTTG AAACAAAAAG TAGAAGCCCT GTTTGATGAG 
AAATGTGGGG AGGCACTTGG TCTTAATGAA CCCTATAAGG TTCCTTTTGC ATTGTTTGAA 
TCATACCCAG AAGATTTCTA CGTGGAAGGG CTTCCTGAGG GTGTTCCCTT CCGCCGGCCG 
TCCACATTTG GTATCCCTAG ACTTGAGAAG ATTCTCAGAA ACAGGGCAAA AATTAAATTT 
GTTATTAAAA AACCTGAAAT GATTGAAAAC ACTGCCAAAG AACAGCCTCC AAACTTTGCA 
CCAAAAAAGG CAGGAATTCA GAACAATGCA CATACAGCTT CTGGACTTGA AGATCTGAAT 
ATAATCCAAG TGACAATACC AGATGCAGAC AATGAAAAAA ACGTTAAAGT AGACAAAGCC 
CGACAGCTGA GGGAGCAAGT CAATGAACTG TTCAGTAAGA AGTTCGGAGA AGCCATTGGC 
ATGAACTTTC CAGTAAAAGT CCCTTACAGG AAAATTACAA CTAACCCAGG ATGTGTGGTA 
GTTGATGGAA TGCCTCCTGG AGTTTCATTC AAAGCCCCCA GTTACCTTGA AATGAGTTCT 
ATGCGTAGGA TTCTGGATTC AGCTGAATTT ATTAAGTTTA CAGTTATAAG GCCTTTTCCA 
GGGCTGGTTC TTAACAACCA AGAAAGTGAA TCCGGACAGT CAGCAGAAAA TGCTGACAGA 
AGTCAAACAG ATGAAGCTGC AGAAGAAGGA TCAAAAGACA CACAAGACTT AAGTCTTATA 
AAACAAGAAC CAGATCCCAC GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gtf2i ( XM_031896976.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752836.1 Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa28123
Clone ID Related Accession (Same CDS sequence)	XM_012957546.2 , XM_031896976.1 , XM_031896975.1
Accession Version	XM_031896976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	general transcription factor II-I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCTG AGGCCTTAGG CAAAACAGAG GCTATGTCTG TGCCATACCA GAAATTTGAA 
GCAAACCCTG CTGAAATGTA TGTGGAAGGG TTGCCTGATA ATATTCCATT CCGTAGCCCT 
TCCTGGTATG GAATTCCAAG ACTGGAAAAG ATAATTCAGG TTGGCAACAG GATCAAATTT 
GTGATTAAAA AACCAGAATT GCTGACTCAG TCTGTAGCTG AATCAATGCA AAACAGAACA 
AATTCAGGAG TGAAAGAAGA CTGGAATGTG AGGATTACTA AATTAAGAAA GCAGGTAGAG 
GAGATCTTCA ATACAAAATT TGCACAAGCA CTGGGACTTC CTGAAGCAGT AAAAGTCCCT 
TATCCAGTAT TTGAGTCTAA CCCAGAGTTC CTGTATGTCG AAGGATTGCC TGAAGGAATT 
CCCTTCCGGA GCCCCACATG GTTTGGGATA CCTCGGCTAG AGAGAATTGT TCGAGGAAGT 
GCGAAAATTA AATTTATAGT TAAGAAACCT CAGCTGGTTG CAACACACAT TCCTTCTGAA 
TTGGTCAACA AAATAAATAT TGATGTGTCA CAATCTACAG AGAGCGGAAG CAATCCAGCA 
AGTAAGCCAC CCGAGGCAGC AGTTGATGTT GATACCTCAA AATCATGCAT ACGGACGACT 
CCTGCCCAGA CTAATGGTTC CAGTATGCCT TTCAAACCCC GGGGAAGAGA ATTTTCTTTT 
GAGGCGTGGA ATGCCAAAAT TACAGACTTG AAACAAAAAG TAGAAGCCCT GTTTGATGAG 
AAATGTGGGG AGGCACTTGG TCTTAATGAA CCCTATAAGG TTCCTTTTGC ATTGTTTGAA 
TCATACCCAG AAGATTTCTA CGTGGAAGGG CTTCCTGAGG GTGTTCCCTT CCGCCGGCCG 
TCCACATTTG GTATCCCTAG ACTTGAGAAG ATTCTCAGAA ACAGGGCAAA AATTAAATTT 
GTTATTAAAA AACCTGAAAT GATTGAAAAC ACTGCCAAAG AACAGCCTCC AAACTTTGCA 
CCAAAAAAGG CAGGAATTCA GAACAATGCA CATACAGCTT CTGGACTTGA AGATCTGAAT 
ATAATCCAAG TGACAATACC AGATGCAGAC AATGAAAAAA ACGTTAAAGT AGACAAAGCC 
CGACAGCTGA GGGAGCAAGT CAATGAACTG TTCAGTAAGA AGTTCGGAGA AGCCATTGGC 
ATGAACTTTC CAGTAAAAGT CCCTTACAGG AAAATTACAA CTAACCCAGG ATGTGTGGTA 
GTTGATGGAA TGCCTCCTGG AGTTTCATTC AAAGCCCCCA GTTACCTTGA AATGAGTTCT 
ATGCGTAGGA TTCTGGATTC AGCTGAATTT ATTAAGTTTA CAGTTATAAG GCCTTTTCCA 
GGGCTGGTTC TTAACAACCA AGAAAGTGAA TCCGGACAGT CAGCAGAAAA TGCTGACAGA 
AGTCAAACAG ATGAAGCTGC AGAAGAAGGA TCAAAAGACA CACAAGACTT AAGTCTTATA 
AAACAAGAAC CAGATCCCAC GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752836.1
CDS	205..3051
Translation	MARFQMPDLPVLNESSDHREAIKHFITAVESMCTEFAKIKAEVACIAVYDHETLIAGSEKGRSFLDARKDFQKAFIQYCKLEEENTPQHHVIISSKAATDCAEIESLQKSVEDLFCICYGKALGKSTPVPVPYDKMVRDPTAVSVTGLPKDVFVKDPSNYDLETVTQILHNRSQVSFSIKRPFLDRRKSDEDAVTDYNDASVSESYNTVQVKTEPSEDSESSLELTTVTIKEEIDDQDYYQFNFQGCGYGEESNEATEVEQREDSALQVPSETSEDPEVEVTIEDDEYIPPNKKPKSTEIVNEPLQFKRKTREFNFEKWNARITELRKQVEELFEKKYAQAINVTGPVAIPYHLFQSNTEDLYVEGLPEGIPFRRPSTYGIPRLERILLARERIRFVIKKHDLLNARDVMPLEKPNTGVKEEWYARITKLRKIVDQLFSKKFAEALGKTEAMSVPYQKFEANPAEMYVEGLPDNIPFRSPSWYGIPRLEKIIQVGNRIKFVIKKPELLTQSVAESMQNRTNSGVKEDWNVRITKLRKQVEEIFNTKFAQALGLPEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSAKIKFIVKKPQLVATHIPSELVNKINIDVSQSTESGSNPASKPPEAAVDVDTSKSCIRTTPAQTNGSSMPFKPRGREFSFEAWNAKITDLKQKVEALFDEKCGEALGLNEPYKVPFALFESYPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNRAKIKFVIKKPEMIENTAKEQPPNFAPKKAGIQNNAHTASGLEDLNIIQVTIPDADNEKNVKVDKARQLREQVNELFSKKFGEAIGMNFPVKVPYRKITTNPGCVVVDGMPPGVSFKAPSYLEMSSMRRILDSAEFIKFTVIRPFPGLVLNNQESESGQSAENADRSQTDEAAEEGSKDTQDLSLIKQEPDPTW

Target ORF information:

RefSeq Version	XM_031896976.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896976.1

ORF Insert Sequence:

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCTG AGGCCTTAGG CAAAACAGAG GCTATGTCTG TGCCATACCA GAAATTTGAA 
GCAAACCCTG CTGAAATGTA TGTGGAAGGG TTGCCTGATA ATATTCCATT CCGTAGCCCT 
TCCTGGTATG GAATTCCAAG ACTGGAAAAG ATAATTCAGG TTGGCAACAG GATCAAATTT 
GTGATTAAAA AACCAGAATT GCTGACTCAG TCTGTAGCTG AATCAATGCA AAACAGAACA 
AATTCAGGAG TGAAAGAAGA CTGGAATGTG AGGATTACTA AATTAAGAAA GCAGGTAGAG 
GAGATCTTCA ATACAAAATT TGCACAAGCA CTGGGACTTC CTGAAGCAGT AAAAGTCCCT 
TATCCAGTAT TTGAGTCTAA CCCAGAGTTC CTGTATGTCG AAGGATTGCC TGAAGGAATT 
CCCTTCCGGA GCCCCACATG GTTTGGGATA CCTCGGCTAG AGAGAATTGT TCGAGGAAGT 
GCGAAAATTA AATTTATAGT TAAGAAACCT CAGCTGGTTG CAACACACAT TCCTTCTGAA 
TTGGTCAACA AAATAAATAT TGATGTGTCA CAATCTACAG AGAGCGGAAG CAATCCAGCA 
AGTAAGCCAC CCGAGGCAGC AGTTGATGTT GATACCTCAA AATCATGCAT ACGGACGACT 
CCTGCCCAGA CTAATGGTTC CAGTATGCCT TTCAAACCCC GGGGAAGAGA ATTTTCTTTT 
GAGGCGTGGA ATGCCAAAAT TACAGACTTG AAACAAAAAG TAGAAGCCCT GTTTGATGAG 
AAATGTGGGG AGGCACTTGG TCTTAATGAA CCCTATAAGG TTCCTTTTGC ATTGTTTGAA 
TCATACCCAG AAGATTTCTA CGTGGAAGGG CTTCCTGAGG GTGTTCCCTT CCGCCGGCCG 
TCCACATTTG GTATCCCTAG ACTTGAGAAG ATTCTCAGAA ACAGGGCAAA AATTAAATTT 
GTTATTAAAA AACCTGAAAT GATTGAAAAC ACTGCCAAAG AACAGCCTCC AAACTTTGCA 
CCAAAAAAGG CAGGAATTCA GAACAATGCA CATACAGCTT CTGGACTTGA AGATCTGAAT 
ATAATCCAAG TGACAATACC AGATGCAGAC AATGAAAAAA ACGTTAAAGT AGACAAAGCC 
CGACAGCTGA GGGAGCAAGT CAATGAACTG TTCAGTAAGA AGTTCGGAGA AGCCATTGGC 
ATGAACTTTC CAGTAAAAGT CCCTTACAGG AAAATTACAA CTAACCCAGG ATGTGTGGTA 
GTTGATGGAA TGCCTCCTGG AGTTTCATTC AAAGCCCCCA GTTACCTTGA AATGAGTTCT 
ATGCGTAGGA TTCTGGATTC AGCTGAATTT ATTAAGTTTA CAGTTATAAG GCCTTTTCCA 
GGGCTGGTTC TTAACAACCA AGAAAGTGAA TCCGGACAGT CAGCAGAAAA TGCTGACAGA 
AGTCAAACAG ATGAAGCTGC AGAAGAAGGA TCAAAAGACA CACAAGACTT AAGTCTTATA 
AAACAAGAAC CAGATCCCAC GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gtf2i ( XM_031896975.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752835.1 Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa28123
Clone ID Related Accession (Same CDS sequence)	XM_012957546.2 , XM_031896976.1 , XM_031896975.1
Accession Version	XM_031896975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	general transcription factor II-I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCTG AGGCCTTAGG CAAAACAGAG GCTATGTCTG TGCCATACCA GAAATTTGAA 
GCAAACCCTG CTGAAATGTA TGTGGAAGGG TTGCCTGATA ATATTCCATT CCGTAGCCCT 
TCCTGGTATG GAATTCCAAG ACTGGAAAAG ATAATTCAGG TTGGCAACAG GATCAAATTT 
GTGATTAAAA AACCAGAATT GCTGACTCAG TCTGTAGCTG AATCAATGCA AAACAGAACA 
AATTCAGGAG TGAAAGAAGA CTGGAATGTG AGGATTACTA AATTAAGAAA GCAGGTAGAG 
GAGATCTTCA ATACAAAATT TGCACAAGCA CTGGGACTTC CTGAAGCAGT AAAAGTCCCT 
TATCCAGTAT TTGAGTCTAA CCCAGAGTTC CTGTATGTCG AAGGATTGCC TGAAGGAATT 
CCCTTCCGGA GCCCCACATG GTTTGGGATA CCTCGGCTAG AGAGAATTGT TCGAGGAAGT 
GCGAAAATTA AATTTATAGT TAAGAAACCT CAGCTGGTTG CAACACACAT TCCTTCTGAA 
TTGGTCAACA AAATAAATAT TGATGTGTCA CAATCTACAG AGAGCGGAAG CAATCCAGCA 
AGTAAGCCAC CCGAGGCAGC AGTTGATGTT GATACCTCAA AATCATGCAT ACGGACGACT 
CCTGCCCAGA CTAATGGTTC CAGTATGCCT TTCAAACCCC GGGGAAGAGA ATTTTCTTTT 
GAGGCGTGGA ATGCCAAAAT TACAGACTTG AAACAAAAAG TAGAAGCCCT GTTTGATGAG 
AAATGTGGGG AGGCACTTGG TCTTAATGAA CCCTATAAGG TTCCTTTTGC ATTGTTTGAA 
TCATACCCAG AAGATTTCTA CGTGGAAGGG CTTCCTGAGG GTGTTCCCTT CCGCCGGCCG 
TCCACATTTG GTATCCCTAG ACTTGAGAAG ATTCTCAGAA ACAGGGCAAA AATTAAATTT 
GTTATTAAAA AACCTGAAAT GATTGAAAAC ACTGCCAAAG AACAGCCTCC AAACTTTGCA 
CCAAAAAAGG CAGGAATTCA GAACAATGCA CATACAGCTT CTGGACTTGA AGATCTGAAT 
ATAATCCAAG TGACAATACC AGATGCAGAC AATGAAAAAA ACGTTAAAGT AGACAAAGCC 
CGACAGCTGA GGGAGCAAGT CAATGAACTG TTCAGTAAGA AGTTCGGAGA AGCCATTGGC 
ATGAACTTTC CAGTAAAAGT CCCTTACAGG AAAATTACAA CTAACCCAGG ATGTGTGGTA 
GTTGATGGAA TGCCTCCTGG AGTTTCATTC AAAGCCCCCA GTTACCTTGA AATGAGTTCT 
ATGCGTAGGA TTCTGGATTC AGCTGAATTT ATTAAGTTTA CAGTTATAAG GCCTTTTCCA 
GGGCTGGTTC TTAACAACCA AGAAAGTGAA TCCGGACAGT CAGCAGAAAA TGCTGACAGA 
AGTCAAACAG ATGAAGCTGC AGAAGAAGGA TCAAAAGACA CACAAGACTT AAGTCTTATA 
AAACAAGAAC CAGATCCCAC GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752835.1
CDS	358..3204
Translation	MARFQMPDLPVLNESSDHREAIKHFITAVESMCTEFAKIKAEVACIAVYDHETLIAGSEKGRSFLDARKDFQKAFIQYCKLEEENTPQHHVIISSKAATDCAEIESLQKSVEDLFCICYGKALGKSTPVPVPYDKMVRDPTAVSVTGLPKDVFVKDPSNYDLETVTQILHNRSQVSFSIKRPFLDRRKSDEDAVTDYNDASVSESYNTVQVKTEPSEDSESSLELTTVTIKEEIDDQDYYQFNFQGCGYGEESNEATEVEQREDSALQVPSETSEDPEVEVTIEDDEYIPPNKKPKSTEIVNEPLQFKRKTREFNFEKWNARITELRKQVEELFEKKYAQAINVTGPVAIPYHLFQSNTEDLYVEGLPEGIPFRRPSTYGIPRLERILLARERIRFVIKKHDLLNARDVMPLEKPNTGVKEEWYARITKLRKIVDQLFSKKFAEALGKTEAMSVPYQKFEANPAEMYVEGLPDNIPFRSPSWYGIPRLEKIIQVGNRIKFVIKKPELLTQSVAESMQNRTNSGVKEDWNVRITKLRKQVEEIFNTKFAQALGLPEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSAKIKFIVKKPQLVATHIPSELVNKINIDVSQSTESGSNPASKPPEAAVDVDTSKSCIRTTPAQTNGSSMPFKPRGREFSFEAWNAKITDLKQKVEALFDEKCGEALGLNEPYKVPFALFESYPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNRAKIKFVIKKPEMIENTAKEQPPNFAPKKAGIQNNAHTASGLEDLNIIQVTIPDADNEKNVKVDKARQLREQVNELFSKKFGEAIGMNFPVKVPYRKITTNPGCVVVDGMPPGVSFKAPSYLEMSSMRRILDSAEFIKFTVIRPFPGLVLNNQESESGQSAENADRSQTDEAAEEGSKDTQDLSLIKQEPDPTW

Target ORF information:

RefSeq Version	XM_031896975.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896975.1

ORF Insert Sequence:

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCTG AGGCCTTAGG CAAAACAGAG GCTATGTCTG TGCCATACCA GAAATTTGAA 
GCAAACCCTG CTGAAATGTA TGTGGAAGGG TTGCCTGATA ATATTCCATT CCGTAGCCCT 
TCCTGGTATG GAATTCCAAG ACTGGAAAAG ATAATTCAGG TTGGCAACAG GATCAAATTT 
GTGATTAAAA AACCAGAATT GCTGACTCAG TCTGTAGCTG AATCAATGCA AAACAGAACA 
AATTCAGGAG TGAAAGAAGA CTGGAATGTG AGGATTACTA AATTAAGAAA GCAGGTAGAG 
GAGATCTTCA ATACAAAATT TGCACAAGCA CTGGGACTTC CTGAAGCAGT AAAAGTCCCT 
TATCCAGTAT TTGAGTCTAA CCCAGAGTTC CTGTATGTCG AAGGATTGCC TGAAGGAATT 
CCCTTCCGGA GCCCCACATG GTTTGGGATA CCTCGGCTAG AGAGAATTGT TCGAGGAAGT 
GCGAAAATTA AATTTATAGT TAAGAAACCT CAGCTGGTTG CAACACACAT TCCTTCTGAA 
TTGGTCAACA AAATAAATAT TGATGTGTCA CAATCTACAG AGAGCGGAAG CAATCCAGCA 
AGTAAGCCAC CCGAGGCAGC AGTTGATGTT GATACCTCAA AATCATGCAT ACGGACGACT 
CCTGCCCAGA CTAATGGTTC CAGTATGCCT TTCAAACCCC GGGGAAGAGA ATTTTCTTTT 
GAGGCGTGGA ATGCCAAAAT TACAGACTTG AAACAAAAAG TAGAAGCCCT GTTTGATGAG 
AAATGTGGGG AGGCACTTGG TCTTAATGAA CCCTATAAGG TTCCTTTTGC ATTGTTTGAA 
TCATACCCAG AAGATTTCTA CGTGGAAGGG CTTCCTGAGG GTGTTCCCTT CCGCCGGCCG 
TCCACATTTG GTATCCCTAG ACTTGAGAAG ATTCTCAGAA ACAGGGCAAA AATTAAATTT 
GTTATTAAAA AACCTGAAAT GATTGAAAAC ACTGCCAAAG AACAGCCTCC AAACTTTGCA 
CCAAAAAAGG CAGGAATTCA GAACAATGCA CATACAGCTT CTGGACTTGA AGATCTGAAT 
ATAATCCAAG TGACAATACC AGATGCAGAC AATGAAAAAA ACGTTAAAGT AGACAAAGCC 
CGACAGCTGA GGGAGCAAGT CAATGAACTG TTCAGTAAGA AGTTCGGAGA AGCCATTGGC 
ATGAACTTTC CAGTAAAAGT CCCTTACAGG AAAATTACAA CTAACCCAGG ATGTGTGGTA 
GTTGATGGAA TGCCTCCTGG AGTTTCATTC AAAGCCCCCA GTTACCTTGA AATGAGTTCT 
ATGCGTAGGA TTCTGGATTC AGCTGAATTT ATTAAGTTTA CAGTTATAAG GCCTTTTCCA 
GGGCTGGTTC TTAACAACCA AGAAAGTGAA TCCGGACAGT CAGCAGAAAA TGCTGACAGA 
AGTCAAACAG ATGAAGCTGC AGAAGAAGGA TCAAAAGACA CACAAGACTT AAGTCTTATA 
AAACAAGAAC CAGATCCCAC GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gtf2i ( XM_031896977.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752837.1 Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa52416
Clone ID Related Accession (Same CDS sequence)	XM_031896977.1
Accession Version	XM_031896977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	general transcription factor II-I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCTG AGGCCTTAGG CAAAACAGAG GCTATGTCTG TGCCATACCA GAAATTTGAA 
GCAAACCCTG CTGAAATGTA TGTGGAAGGG TTGCCTGATA ATATTCCATT CCGTAGCCCT 
TCCTGGTATG GAATTCCAAG ACTGGAAAAG ATAATTCAGG TTGGCAACAG GATCAAATTT 
GTGATTAAAA AACCAGAATT GCTGACTCAG TCTGTAGCTG AATCAATGCA AAACAGAACA 
AATTCAGGAG TGAAAGAAGA CTGGAATGTG AGGATTACTA AATTAAGAAA GCAGGTAGAG 
GAGATCTTCA ATACAAAATT TGCACAAGCA CTGGGACTTC CTGAAGCAGT AAAAGTCCCT 
TATCCAGTAT TTGAGTCTAA CCCAGAGTTC CTGTATGTCG AAGGATTGCC TGAAGGAATT 
CCCTTCCGGA GCCCCACATG GTTTGGGATA CCTCGGCTAG AGAGAATTGT TCGAGGAAGT 
GCGAAAATTA AATTTATAGT TAAGAAACCT CAGCTGGTTG CAACACACAT TCCTTCTGAA 
TTGGTCAACA AAATAAATAT TGATGTGTCA CAATCTACAG AGAGCGGAAG CAATCCAGCA 
AGTAAGCCAC CCGAGGCAGC AGTTGATGTT GATACCTCAA AATCATGCAT ACGGACGACT 
CCTGCCCAGA CTAATGGTTC CAGTATGCCT TTCAAACCCC GGGGAAGAGA ATTTTCTTTT 
GAGGCGTGGA ATGCCAAAAT TACAGACTTG AAACAAAAAG TAGAAGCCCT GTTTGATGAG 
AAATGTGGGG AGGCACTTGG TCTTAATGAA CCCTATAAGG TTCCTTTTGC ATTGTTTGAA 
TCATACCCAG AAGATTTCTA CGTGGAAGGG CTTCCTGAGG GTGTTCCCTT CCGCCGGCCG 
TCCACATTTG GTATCCCTAG ACTTGAGAAG ATTCTCAGAA ACAGGGCAAA AATTAAATTT 
GTTATTAAAA AACCTGAAAT GATTGAAAAC ACTGCCAAAG AACAGCCTCC AAACTTTGCA 
CCAAAAAAGG CAGGAATTCA GAACAATGCA CATACAGCTT CTGGACTTGA AGATCTGAAT 
ATAATCCAAG TGACAATACC AGATGCAGAC AATGAAAAAA ACGTTAAAGT AGACAAAGCC 
CGACAGCTGA GGGAGCAAGT CAATGAACTG TTCAGTAAGA AGTTCGGAGA AGCCATTGGC 
ATGAACTTTC CAGTAAAAGT CCCTTACAGG AAAATTACAA CTAACCCAGG ATGTGTGGTA 
GTTGATGGAA TGCCTCCTGG AGTTTCATTC AAAGCCCCCA GTTACCTTGA AATGAGTTCT 
ATGCGTAGGA TTCTGGATTC AGCTGAATTT ATTAAGTTTA CAGTTATAAG GCCTTTTCCA 
GGGCTGGTTC TTAACAACCA AAGTGAATCC GGACAGTCAG CAGAAAATGC TGACAGAAGT 
CAAACAGATG AAGCTGCAGA AGAAGGATCA AAAGACACAC AAGACTTAAG TCTTATAAAA 
CAAGAACCAG ATCCCACGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752837.1
CDS	360..3203
Translation	MARFQMPDLPVLNESSDHREAIKHFITAVESMCTEFAKIKAEVACIAVYDHETLIAGSEKGRSFLDARKDFQKAFIQYCKLEEENTPQHHVIISSKAATDCAEIESLQKSVEDLFCICYGKALGKSTPVPVPYDKMVRDPTAVSVTGLPKDVFVKDPSNYDLETVTQILHNRSQVSFSIKRPFLDRRKSDEDAVTDYNDASVSESYNTVQVKTEPSEDSESSLELTTVTIKEEIDDQDYYQFNFQGCGYGEESNEATEVEQREDSALQVPSETSEDPEVEVTIEDDEYIPPNKKPKSTEIVNEPLQFKRKTREFNFEKWNARITELRKQVEELFEKKYAQAINVTGPVAIPYHLFQSNTEDLYVEGLPEGIPFRRPSTYGIPRLERILLARERIRFVIKKHDLLNARDVMPLEKPNTGVKEEWYARITKLRKIVDQLFSKKFAEALGKTEAMSVPYQKFEANPAEMYVEGLPDNIPFRSPSWYGIPRLEKIIQVGNRIKFVIKKPELLTQSVAESMQNRTNSGVKEDWNVRITKLRKQVEEIFNTKFAQALGLPEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSAKIKFIVKKPQLVATHIPSELVNKINIDVSQSTESGSNPASKPPEAAVDVDTSKSCIRTTPAQTNGSSMPFKPRGREFSFEAWNAKITDLKQKVEALFDEKCGEALGLNEPYKVPFALFESYPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNRAKIKFVIKKPEMIENTAKEQPPNFAPKKAGIQNNAHTASGLEDLNIIQVTIPDADNEKNVKVDKARQLREQVNELFSKKFGEAIGMNFPVKVPYRKITTNPGCVVVDGMPPGVSFKAPSYLEMSSMRRILDSAEFIKFTVIRPFPGLVLNNQSESGQSAENADRSQTDEAAEEGSKDTQDLSLIKQEPDPTW

Target ORF information:

RefSeq Version	XM_031896977.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896977.1

ORF Insert Sequence:

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCTG AGGCCTTAGG CAAAACAGAG GCTATGTCTG TGCCATACCA GAAATTTGAA 
GCAAACCCTG CTGAAATGTA TGTGGAAGGG TTGCCTGATA ATATTCCATT CCGTAGCCCT 
TCCTGGTATG GAATTCCAAG ACTGGAAAAG ATAATTCAGG TTGGCAACAG GATCAAATTT 
GTGATTAAAA AACCAGAATT GCTGACTCAG TCTGTAGCTG AATCAATGCA AAACAGAACA 
AATTCAGGAG TGAAAGAAGA CTGGAATGTG AGGATTACTA AATTAAGAAA GCAGGTAGAG 
GAGATCTTCA ATACAAAATT TGCACAAGCA CTGGGACTTC CTGAAGCAGT AAAAGTCCCT 
TATCCAGTAT TTGAGTCTAA CCCAGAGTTC CTGTATGTCG AAGGATTGCC TGAAGGAATT 
CCCTTCCGGA GCCCCACATG GTTTGGGATA CCTCGGCTAG AGAGAATTGT TCGAGGAAGT 
GCGAAAATTA AATTTATAGT TAAGAAACCT CAGCTGGTTG CAACACACAT TCCTTCTGAA 
TTGGTCAACA AAATAAATAT TGATGTGTCA CAATCTACAG AGAGCGGAAG CAATCCAGCA 
AGTAAGCCAC CCGAGGCAGC AGTTGATGTT GATACCTCAA AATCATGCAT ACGGACGACT 
CCTGCCCAGA CTAATGGTTC CAGTATGCCT TTCAAACCCC GGGGAAGAGA ATTTTCTTTT 
GAGGCGTGGA ATGCCAAAAT TACAGACTTG AAACAAAAAG TAGAAGCCCT GTTTGATGAG 
AAATGTGGGG AGGCACTTGG TCTTAATGAA CCCTATAAGG TTCCTTTTGC ATTGTTTGAA 
TCATACCCAG AAGATTTCTA CGTGGAAGGG CTTCCTGAGG GTGTTCCCTT CCGCCGGCCG 
TCCACATTTG GTATCCCTAG ACTTGAGAAG ATTCTCAGAA ACAGGGCAAA AATTAAATTT 
GTTATTAAAA AACCTGAAAT GATTGAAAAC ACTGCCAAAG AACAGCCTCC AAACTTTGCA 
CCAAAAAAGG CAGGAATTCA GAACAATGCA CATACAGCTT CTGGACTTGA AGATCTGAAT 
ATAATCCAAG TGACAATACC AGATGCAGAC AATGAAAAAA ACGTTAAAGT AGACAAAGCC 
CGACAGCTGA GGGAGCAAGT CAATGAACTG TTCAGTAAGA AGTTCGGAGA AGCCATTGGC 
ATGAACTTTC CAGTAAAAGT CCCTTACAGG AAAATTACAA CTAACCCAGG ATGTGTGGTA 
GTTGATGGAA TGCCTCCTGG AGTTTCATTC AAAGCCCCCA GTTACCTTGA AATGAGTTCT 
ATGCGTAGGA TTCTGGATTC AGCTGAATTT ATTAAGTTTA CAGTTATAAG GCCTTTTCCA 
GGGCTGGTTC TTAACAACCA AAGTGAATCC GGACAGTCAG CAGAAAATGC TGACAGAAGT 
CAAACAGATG AAGCTGCAGA AGAAGGATCA AAAGACACAC AAGACTTAAG TCTTATAAAA 
CAAGAACCAG ATCCCACGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gtf2i ( XM_031896978.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752838.1 Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa52417
Clone ID Related Accession (Same CDS sequence)	XM_031896978.1
Accession Version	XM_031896978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	general transcription factor II-I isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATGATGAGTA CATTCCACCT AATAAGAAGC CAAAGAGCAC AGAAATTGTC 
AATGAACCAC TTCAATTTAA ACGAAAAACC CGGGAGTTTA ACTTTGAAAA GTGGAATGCT 
AGAATTACAG AGTTAAGAAA GCAAGTGGAA GAATTATTTG AGAAAAAATA TGCTCAAGCT 
ATTAATGTGA CTGGTCCTGT CGCAATCCCA TATCACCTTT TTCAGTCCAA TACTGAAGAT 
CTGTATGTTG AAGGTTTACC AGAAGGAATT CCATTCAGAA GGCCTTCAAC CTATGGAATT 
CCACGCCTCG AAAGGATATT GCTAGCAAGG GAAAGGATAC GATTTGTCAT TAAAAAGCAT 
GATTTGCTAA ATGCAAGAGA TGTTATGCCG CTTGAAAAGC CCAACACAGG AGTGAAAGAA 
GAATGGTATG CTAGAATAAC GAAATTAAGA AAAATCGTTG ATCAGTTGTT CAGTAAAAAA 
TTTGCTGAGG CCTTAGGCAA AACAGAGGCT ATGTCTGTGC CATACCAGAA ATTTGAAGCA 
AACCCTGCTG AAATGTATGT GGAAGGGTTG CCTGATAATA TTCCATTCCG TAGCCCTTCC 
TGGTATGGAA TTCCAAGACT GGAAAAGATA ATTCAGGTTG GCAACAGGAT CAAATTTGTG 
ATTAAAAAAC CAGAATTGCT GACTCAGTCT GTAGCTGAAT CAATGCAAAA CAGAACAAAT 
TCAGGAGTGA AAGAAGACTG GAATGTGAGG ATTACTAAAT TAAGAAAGCA GGTAGAGGAG 
ATCTTCAATA CAAAATTTGC ACAAGCACTG GGACTTCCTG AAGCAGTAAA AGTCCCTTAT 
CCAGTATTTG AGTCTAACCC AGAGTTCCTG TATGTCGAAG GATTGCCTGA AGGAATTCCC 
TTCCGGAGCC CCACATGGTT TGGGATACCT CGGCTAGAGA GAATTGTTCG AGGAAGTGCG 
AAAATTAAAT TTATAGTTAA GAAACCTCAG CTGGTTGCAA CACACATTCC TTCTGAATTG 
GTCAACAAAA TAAATATTGA TGTGTCACAA TCTACAGAGA GCGGAAGCAA TCCAGCAAGT 
AAGCCACCCG AGGCAGCAGT TGATGTTGAT ACCTCAAAAT CATGCATACG GACGACTCCT 
GCCCAGACTA ATGGTTCCAG TATGCCTTTC AAACCCCGGG GAAGAGAATT TTCTTTTGAG 
GCGTGGAATG CCAAAATTAC AGACTTGAAA CAAAAAGTAG AAGCCCTGTT TGATGAGAAA 
TGTGGGGAGG CACTTGGTCT TAATGAACCC TATAAGGTTC CTTTTGCATT GTTTGAATCA 
TACCCAGAAG ATTTCTACGT GGAAGGGCTT CCTGAGGGTG TTCCCTTCCG CCGGCCGTCC 
ACATTTGGTA TCCCTAGACT TGAGAAGATT CTCAGAAACA GGGCAAAAAT TAAATTTGTT 
ATTAAAAAAC CTGAAATGAT TGAAAACACT GCCAAAGAAC AGCCTCCAAA CTTTGCACCA 
AAAAAGGCAG GAATTCAGAA CAATGCACAT ACAGCTTCTG GACTTGAAGA TCTGAATATA 
ATCCAAGTGA CAATACCAGA TGCAGACAAT GAAAAAAACG TTAAAGTAGA CAAAGCCCGA 
CAGCTGAGGG AGCAAGTCAA TGAACTGTTC AGTAAGAAGT TCGGAGAAGC CATTGGCATG 
AACTTTCCAG TAAAAGTCCC TTACAGGAAA ATTACAACTA ACCCAGGATG TGTGGTAGTT 
GATGGAATGC CTCCTGGAGT TTCATTCAAA GCCCCCAGTT ACCTTGAAAT GAGTTCTATG 
CGTAGGATTC TGGATTCAGC TGAATTTATT AAGTTTACAG TTATAAGGCC TTTTCCAGGG 
CTGGTTCTTA ACAACCAAGA AAGTGAATCC GGACAGTCAG CAGAAAATGC TGACAGAAGT 
CAAACAGATG AAGCTGCAGA AGAAGGATCA AAAGACACAC AAGACTTAAG TCTTATAAAA 
CAAGAACCAG ATCCCACGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752838.1
CDS	357..3140
Translation	MARFQMPDLPVLNESSDHREAIKHFITAVESMCTEFAKIKAEVACIAVYDHETLIAGSEKGRSFLDARKDFQKAFIQYCKLEEENTPQHHVIISSKAATDCAEIESLQKSVEDLFCICYGKALGKSTPVPVPYDKMVRDPTAVSVTGLPKDVFVKDPSNYDLETVTQILHNRSQVSFSIKRPFLDRRKSDEDAVTDYNDASVSESYNTVQVKTEPSEDSESSLELTTVTIKEEIDDQDYYQFNFQGCGYGEESNEATEVEQREDDEYIPPNKKPKSTEIVNEPLQFKRKTREFNFEKWNARITELRKQVEELFEKKYAQAINVTGPVAIPYHLFQSNTEDLYVEGLPEGIPFRRPSTYGIPRLERILLARERIRFVIKKHDLLNARDVMPLEKPNTGVKEEWYARITKLRKIVDQLFSKKFAEALGKTEAMSVPYQKFEANPAEMYVEGLPDNIPFRSPSWYGIPRLEKIIQVGNRIKFVIKKPELLTQSVAESMQNRTNSGVKEDWNVRITKLRKQVEEIFNTKFAQALGLPEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSAKIKFIVKKPQLVATHIPSELVNKINIDVSQSTESGSNPASKPPEAAVDVDTSKSCIRTTPAQTNGSSMPFKPRGREFSFEAWNAKITDLKQKVEALFDEKCGEALGLNEPYKVPFALFESYPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNRAKIKFVIKKPEMIENTAKEQPPNFAPKKAGIQNNAHTASGLEDLNIIQVTIPDADNEKNVKVDKARQLREQVNELFSKKFGEAIGMNFPVKVPYRKITTNPGCVVVDGMPPGVSFKAPSYLEMSSMRRILDSAEFIKFTVIRPFPGLVLNNQESESGQSAENADRSQTDEAAEEGSKDTQDLSLIKQEPDPTW

Target ORF information:

RefSeq Version	XM_031896978.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896978.1

ORF Insert Sequence:

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATGATGAGTA CATTCCACCT AATAAGAAGC CAAAGAGCAC AGAAATTGTC 
AATGAACCAC TTCAATTTAA ACGAAAAACC CGGGAGTTTA ACTTTGAAAA GTGGAATGCT 
AGAATTACAG AGTTAAGAAA GCAAGTGGAA GAATTATTTG AGAAAAAATA TGCTCAAGCT 
ATTAATGTGA CTGGTCCTGT CGCAATCCCA TATCACCTTT TTCAGTCCAA TACTGAAGAT 
CTGTATGTTG AAGGTTTACC AGAAGGAATT CCATTCAGAA GGCCTTCAAC CTATGGAATT 
CCACGCCTCG AAAGGATATT GCTAGCAAGG GAAAGGATAC GATTTGTCAT TAAAAAGCAT 
GATTTGCTAA ATGCAAGAGA TGTTATGCCG CTTGAAAAGC CCAACACAGG AGTGAAAGAA 
GAATGGTATG CTAGAATAAC GAAATTAAGA AAAATCGTTG ATCAGTTGTT CAGTAAAAAA 
TTTGCTGAGG CCTTAGGCAA AACAGAGGCT ATGTCTGTGC CATACCAGAA ATTTGAAGCA 
AACCCTGCTG AAATGTATGT GGAAGGGTTG CCTGATAATA TTCCATTCCG TAGCCCTTCC 
TGGTATGGAA TTCCAAGACT GGAAAAGATA ATTCAGGTTG GCAACAGGAT CAAATTTGTG 
ATTAAAAAAC CAGAATTGCT GACTCAGTCT GTAGCTGAAT CAATGCAAAA CAGAACAAAT 
TCAGGAGTGA AAGAAGACTG GAATGTGAGG ATTACTAAAT TAAGAAAGCA GGTAGAGGAG 
ATCTTCAATA CAAAATTTGC ACAAGCACTG GGACTTCCTG AAGCAGTAAA AGTCCCTTAT 
CCAGTATTTG AGTCTAACCC AGAGTTCCTG TATGTCGAAG GATTGCCTGA AGGAATTCCC 
TTCCGGAGCC CCACATGGTT TGGGATACCT CGGCTAGAGA GAATTGTTCG AGGAAGTGCG 
AAAATTAAAT TTATAGTTAA GAAACCTCAG CTGGTTGCAA CACACATTCC TTCTGAATTG 
GTCAACAAAA TAAATATTGA TGTGTCACAA TCTACAGAGA GCGGAAGCAA TCCAGCAAGT 
AAGCCACCCG AGGCAGCAGT TGATGTTGAT ACCTCAAAAT CATGCATACG GACGACTCCT 
GCCCAGACTA ATGGTTCCAG TATGCCTTTC AAACCCCGGG GAAGAGAATT TTCTTTTGAG 
GCGTGGAATG CCAAAATTAC AGACTTGAAA CAAAAAGTAG AAGCCCTGTT TGATGAGAAA 
TGTGGGGAGG CACTTGGTCT TAATGAACCC TATAAGGTTC CTTTTGCATT GTTTGAATCA 
TACCCAGAAG ATTTCTACGT GGAAGGGCTT CCTGAGGGTG TTCCCTTCCG CCGGCCGTCC 
ACATTTGGTA TCCCTAGACT TGAGAAGATT CTCAGAAACA GGGCAAAAAT TAAATTTGTT 
ATTAAAAAAC CTGAAATGAT TGAAAACACT GCCAAAGAAC AGCCTCCAAA CTTTGCACCA 
AAAAAGGCAG GAATTCAGAA CAATGCACAT ACAGCTTCTG GACTTGAAGA TCTGAATATA 
ATCCAAGTGA CAATACCAGA TGCAGACAAT GAAAAAAACG TTAAAGTAGA CAAAGCCCGA 
CAGCTGAGGG AGCAAGTCAA TGAACTGTTC AGTAAGAAGT TCGGAGAAGC CATTGGCATG 
AACTTTCCAG TAAAAGTCCC TTACAGGAAA ATTACAACTA ACCCAGGATG TGTGGTAGTT 
GATGGAATGC CTCCTGGAGT TTCATTCAAA GCCCCCAGTT ACCTTGAAAT GAGTTCTATG 
CGTAGGATTC TGGATTCAGC TGAATTTATT AAGTTTACAG TTATAAGGCC TTTTCCAGGG 
CTGGTTCTTA ACAACCAAGA AAGTGAATCC GGACAGTCAG CAGAAAATGC TGACAGAAGT 
CAAACAGATG AAGCTGCAGA AGAAGGATCA AAAGACACAC AAGACTTAAG TCTTATAAAA 
CAAGAACCAG ATCCCACGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gtf2i ( XM_031896979.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752839.1 Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa52418
Clone ID Related Accession (Same CDS sequence)	XM_031896979.1
Accession Version	XM_031896979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	general transcription factor II-I isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCAC AAGCACTGGG ACTTCCTGAA GCAGTAAAAG TCCCTTATCC AGTATTTGAG 
TCTAACCCAG AGTTCCTGTA TGTCGAAGGA TTGCCTGAAG GAATTCCCTT CCGGAGCCCC 
ACATGGTTTG GGATACCTCG GCTAGAGAGA ATTGTTCGAG GAAGTGCGAA AATTAAATTT 
ATAGTTAAGA AACCTCAGCT GGTTGCAACA CACATTCCTT CTGAATTGGT CAACAAAATA 
AATATTGATG TGTCACAATC TACAGAGAGC GGAAGCAATC CAGCAAGTAA GCCACCCGAG 
GCAGCAGTTG ATGTTGATAC CTCAAAATCA TGCATACGGA CGACTCCTGC CCAGACTAAT 
GGTTCCAGTA TGCCTTTCAA ACCCCGGGGA AGAGAATTTT CTTTTGAGGC GTGGAATGCC 
AAAATTACAG ACTTGAAACA AAAAGTAGAA GCCCTGTTTG ATGAGAAATG TGGGGAGGCA 
CTTGGTCTTA ATGAACCCTA TAAGGTTCCT TTTGCATTGT TTGAATCATA CCCAGAAGAT 
TTCTACGTGG AAGGGCTTCC TGAGGGTGTT CCCTTCCGCC GGCCGTCCAC ATTTGGTATC 
CCTAGACTTG AGAAGATTCT CAGAAACAGG GCAAAAATTA AATTTGTTAT TAAAAAACCT 
GAAATGATTG AAAACACTGC CAAAGAACAG CCTCCAAACT TTGCACCAAA AAAGGCAGGA 
ATTCAGAACA ATGCACATAC AGCTTCTGGA CTTGAAGATC TGAATATAAT CCAAGTGACA 
ATACCAGATG CAGACAATGA AAAAAACGTT AAAGTAGACA AAGCCCGACA GCTGAGGGAG 
CAAGTCAATG AACTGTTCAG TAAGAAGTTC GGAGAAGCCA TTGGCATGAA CTTTCCAGTA 
AAAGTCCCTT ACAGGAAAAT TACAACTAAC CCAGGATGTG TGGTAGTTGA TGGAATGCCT 
CCTGGAGTTT CATTCAAAGC CCCCAGTTAC CTTGAAATGA GTTCTATGCG TAGGATTCTG 
GATTCAGCTG AATTTATTAA GTTTACAGTT ATAAGGCCTT TTCCAGGGCT GGTTCTTAAC 
AACCAAGAAA GTGAATCCGG ACAGTCAGCA GAAAATGCTG ACAGAAGTCA AACAGATGAA 
GCTGCAGAAG AAGGATCAAA AGACACACAA GACTTAAGTC TTATAAAACA AGAACCAGAT 
CCCACGTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752839.1
CDS	359..2890
Translation	MARFQMPDLPVLNESSDHREAIKHFITAVESMCTEFAKIKAEVACIAVYDHETLIAGSEKGRSFLDARKDFQKAFIQYCKLEEENTPQHHVIISSKAATDCAEIESLQKSVEDLFCICYGKALGKSTPVPVPYDKMVRDPTAVSVTGLPKDVFVKDPSNYDLETVTQILHNRSQVSFSIKRPFLDRRKSDEDAVTDYNDASVSESYNTVQVKTEPSEDSESSLELTTVTIKEEIDDQDYYQFNFQGCGYGEESNEATEVEQREDSALQVPSETSEDPEVEVTIEDDEYIPPNKKPKSTEIVNEPLQFKRKTREFNFEKWNARITELRKQVEELFEKKYAQAINVTGPVAIPYHLFQSNTEDLYVEGLPEGIPFRRPSTYGIPRLERILLARERIRFVIKKHDLLNARDVMPLEKPNTGVKEEWYARITKLRKIVDQLFSKKFAQALGLPEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSAKIKFIVKKPQLVATHIPSELVNKINIDVSQSTESGSNPASKPPEAAVDVDTSKSCIRTTPAQTNGSSMPFKPRGREFSFEAWNAKITDLKQKVEALFDEKCGEALGLNEPYKVPFALFESYPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNRAKIKFVIKKPEMIENTAKEQPPNFAPKKAGIQNNAHTASGLEDLNIIQVTIPDADNEKNVKVDKARQLREQVNELFSKKFGEAIGMNFPVKVPYRKITTNPGCVVVDGMPPGVSFKAPSYLEMSSMRRILDSAEFIKFTVIRPFPGLVLNNQESESGQSAENADRSQTDEAAEEGSKDTQDLSLIKQEPDPTW

Target ORF information:

RefSeq Version	XM_031896979.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis general transcription factor IIi (gtf2i), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896979.1

ORF Insert Sequence:

ATGGCCCGTT TCCAGATGCC TGACTTACCA GTCCTAAATG AGTCTTCTGA CCACAGAGAA 
GCTATCAAGC ACTTTATTAC TGCAGTGGAG TCAATGTGTA CAGAGTTTGC CAAAATCAAG 
GCAGAAGTAG CATGTATCGC TGTGTACGAT CATGAAACGC TAATAGCTGG CAGTGAAAAA 
GGAAGATCCT TTTTAGATGC AAGAAAGGAT TTCCAGAAAG CCTTTATTCA GTACTGCAAG 
TTGGAGGAAG AGAACACCCC ACAACATCAT GTCATTATAA GTTCTAAGGC AGCAACAGAC 
TGTGCAGAAA TAGAATCTCT CCAAAAGTCT GTGGAGGACT TGTTTTGTAT TTGCTATGGC 
AAAGCTTTAG GAAAATCAAC CCCAGTGCCT GTGCCATATG ACAAGATGGT AAGAGACCCA 
ACTGCAGTCT CAGTAACTGG ACTTCCCAAG GATGTATTTG TTAAAGATCC ATCTAACTAT 
GACCTTGAGA CTGTAACCCA GATATTACAC AACAGATCGC AAGTGTCATT CTCTATTAAA 
AGACCTTTTC TTGATAGGAG GAAATCGGAT GAGGATGCGG TTACAGATTA TAATGATGCC 
TCTGTCTCTG AGAGCTACAA TACTGTGCAA GTGAAAACTG AGCCCAGTGA AGATTCTGAA 
AGTTCACTTG AGTTGACCAC TGTCACTATA AAGGAAGAAA TTGACGATCA AGACTACTAT 
CAGTTTAATT TCCAAGGTTG TGGATATGGT GAAGAGTCCA ATGAAGCAAC TGAAGTGGAG 
CAAAGAGAAG ATTCAGCTCT GCAAGTACCT TCTGAAACAA GTGAGGACCC AGAAGTTGAG 
GTCACTATAG AAGATGATGA GTACATTCCA CCTAATAAGA AGCCAAAGAG CACAGAAATT 
GTCAATGAAC CACTTCAATT TAAACGAAAA ACCCGGGAGT TTAACTTTGA AAAGTGGAAT 
GCTAGAATTA CAGAGTTAAG AAAGCAAGTG GAAGAATTAT TTGAGAAAAA ATATGCTCAA 
GCTATTAATG TGACTGGTCC TGTCGCAATC CCATATCACC TTTTTCAGTC CAATACTGAA 
GATCTGTATG TTGAAGGTTT ACCAGAAGGA ATTCCATTCA GAAGGCCTTC AACCTATGGA 
ATTCCACGCC TCGAAAGGAT ATTGCTAGCA AGGGAAAGGA TACGATTTGT CATTAAAAAG 
CATGATTTGC TAAATGCAAG AGATGTTATG CCGCTTGAAA AGCCCAACAC AGGAGTGAAA 
GAAGAATGGT ATGCTAGAAT AACGAAATTA AGAAAAATCG TTGATCAGTT GTTCAGTAAA 
AAATTTGCAC AAGCACTGGG ACTTCCTGAA GCAGTAAAAG TCCCTTATCC AGTATTTGAG 
TCTAACCCAG AGTTCCTGTA TGTCGAAGGA TTGCCTGAAG GAATTCCCTT CCGGAGCCCC 
ACATGGTTTG GGATACCTCG GCTAGAGAGA ATTGTTCGAG GAAGTGCGAA AATTAAATTT 
ATAGTTAAGA AACCTCAGCT GGTTGCAACA CACATTCCTT CTGAATTGGT CAACAAAATA 
AATATTGATG TGTCACAATC TACAGAGAGC GGAAGCAATC CAGCAAGTAA GCCACCCGAG 
GCAGCAGTTG ATGTTGATAC CTCAAAATCA TGCATACGGA CGACTCCTGC CCAGACTAAT 
GGTTCCAGTA TGCCTTTCAA ACCCCGGGGA AGAGAATTTT CTTTTGAGGC GTGGAATGCC 
AAAATTACAG ACTTGAAACA AAAAGTAGAA GCCCTGTTTG ATGAGAAATG TGGGGAGGCA 
CTTGGTCTTA ATGAACCCTA TAAGGTTCCT TTTGCATTGT TTGAATCATA CCCAGAAGAT 
TTCTACGTGG AAGGGCTTCC TGAGGGTGTT CCCTTCCGCC GGCCGTCCAC ATTTGGTATC 
CCTAGACTTG AGAAGATTCT CAGAAACAGG GCAAAAATTA AATTTGTTAT TAAAAAACCT 
GAAATGATTG AAAACACTGC CAAAGAACAG CCTCCAAACT TTGCACCAAA AAAGGCAGGA 
ATTCAGAACA ATGCACATAC AGCTTCTGGA CTTGAAGATC TGAATATAAT CCAAGTGACA 
ATACCAGATG CAGACAATGA AAAAAACGTT AAAGTAGACA AAGCCCGACA GCTGAGGGAG 
CAAGTCAATG AACTGTTCAG TAAGAAGTTC GGAGAAGCCA TTGGCATGAA CTTTCCAGTA 
AAAGTCCCTT ACAGGAAAAT TACAACTAAC CCAGGATGTG TGGTAGTTGA TGGAATGCCT 
CCTGGAGTTT CATTCAAAGC CCCCAGTTAC CTTGAAATGA GTTCTATGCG TAGGATTCTG 
GATTCAGCTG AATTTATTAA GTTTACAGTT ATAAGGCCTT TTCCAGGGCT GGTTCTTAAC 
AACCAAGAAA GTGAATCCGG ACAGTCAGCA GAAAATGCTG ACAGAAGTCA AACAGATGAA 
GCTGCAGAAG AAGGATCAAA AGACACACAA GACTTAAGTC TTATAAAACA AGAACCAGAT 
CCCACGTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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gtf2i cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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