acsl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	acsl1
Entrez Gene ID	448565
Full Name	acyl-CoA synthetase long chain family member 1 protein
Synonyms	acs1,acsl6,facl1,facl2,lacs,lacs1,lacs2
General protein information	Preferred Names acyl-CoA synthetase long chain family member 1 protein Names long-chain-fatty-acid--CoA ligase 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012960017.2	XP_012815471.1	long-chain-fatty-acid--CoA ligase 1 isoform X2
XM_012960022.3	XP_012815476.1	long-chain-fatty-acid--CoA ligase 1 isoform X2
XM_031898087.1	XP_031753947.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_018092309.1	XP_017947798.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
NM_001006829.1	NP_001006830.1	long-chain-fatty-acid--CoA ligase 1
XM_012960022.2	XP_012815476.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_012960017.3	XP_012815471.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_031898088.1	XP_031753948.1	long-chain-fatty-acid--CoA ligase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACSL1	NP_001273639.1
Mus musculus (house mouse)	Acsl1	NP_032007.2
	ACSL1	XP_001083742.2
Gallus gallus (chicken)	ACSL1	NP_001012596.1
Arabidopsis thaliana (thale cress)	LACS7	NP_198112.2
Xenopus tropicalis (tropical clawed frog)	acsl1	NP_001006830.1
Canis lupus familiaris (dog)	ACSL1	XP_005630044.1
Pan troglodytes (chimpanzee)	ACSL1	XP_003310629.1
Bos taurus (cattle)	ACSL1	NP_001069553.1
Rattus norvegicus (Norway rat)	Acsl1	NP_036952.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following acsl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acsl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa01904	NM_001006829.1 Latest version!	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa01904	XM_018092309.1 Latest version!	Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa01904	XM_012960022.2 Latest version!	Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa26700	XM_012960017.2 Latest version!	Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa47803	XM_031898087.1 Latest version!	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa47803	XM_012960017.3 Latest version!	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa47803	XM_031898088.1 Latest version!	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa47804	XM_012960022.3 Latest version!	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acsl1 ( NM_001006829.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001006830.1 Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), mRNA.

Price & Availability↑

CloneID	OXa01904
Clone ID Related Accession (Same CDS sequence)	XM_018092309.1 , NM_001006829.1 , XM_012960022.2
Accession Version	NM_001006829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	long-chain-fatty-acid--CoA ligase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076898.1. ##Evidence-Data-START## Transcript exon combination :: BC076898.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGGACTT GATGTTTCCT TCTACTCAAG ATGTTCTGAT TTCTTTTCTG 
CCCTTGGCGC ATATGTTTGA AAGAGTCGTG GAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGT 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006830.1
CDS	22..2118
Translation	MQAHEILRNLRIPELGDVRQYVRTLPTNTLMGFGAFAALTTYWYATRPKALKPPCDLAMQSVETQGGEHARRSTILDSDEVITYYYDDVKTIYDIFQRGRRVSNNGPCLGHRKPNQPYEWISYKEVAERAEYVGSALLHRGFKPSPDQFVGIFAQNRPEWTIVELGCYTYSMVAVPLYDTLGAEAITYIINKADLSVVFCDSPEKAKLLLSNVEKGETPVLRTIVLMNPFDNDLVERGKKCGVELVSLKDIEEEGKANREKPKPPKPDDLAVVCFTSGTTGNPKGAMLSHKNIVSNSAGFMKVTEDLMFPSTQDVLISFLPLAHMFERVVECVVLCHGARIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTPVKRWMLDFASKRKEAELRSGIVRNDSFWDKIIFHKVQASLGGKVRLMVTGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSLTIPGDWTAGHVGAPMPCNHVKLVDVEEMNYFASKGEGEVCIKGSNVFQGYLKDDEKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYTRSEPVVQVFVHGESLQAFLVGIVVPDPDNVMNWAKKRKFEGSYEELCKNKDFKNAVLEDLLKLGKEAGLKTFEQVKDIALHSEMFAIENGLLTPTLKAKRPELRKYFKDEIDELYANNKV

Target ORF information:

RefSeq Version	NM_001006829.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006829.1

ORF Insert Sequence:

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGGACTT GATGTTTCCT TCTACTCAAG ATGTTCTGAT TTCTTTTCTG 
CCCTTGGCGC ATATGTTTGA AAGAGTCGTG GAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGT 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acsl1 ( XM_018092309.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947798.1 Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa01904
Clone ID Related Accession (Same CDS sequence)	XM_018092309.1 , NM_001006829.1 , XM_012960022.2
Accession Version	XM_018092309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGGACTT GATGTTTCCT TCTACTCAAG ATGTTCTGAT TTCTTTTCTG 
CCCTTGGCGC ATATGTTTGA AAGAGTCGTG GAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGT 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947798.1
CDS	194..2290
Translation	MQAHEILRNLRIPELGDVRQYVRTLPTNTLMGFGAFAALTTYWYATRPKALKPPCDLAMQSVETQGGEHARRSTILDSDEVITYYYDDVKTIYDIFQRGRRVSNNGPCLGHRKPNQPYEWISYKEVAERAEYVGSALLHRGFKPSPDQFVGIFAQNRPEWTIVELGCYTYSMVAVPLYDTLGAEAITYIINKADLSVVFCDSPEKAKLLLSNVEKGETPVLRTIVLMNPFDNDLVERGKKCGVELVSLKDIEEEGKANREKPKPPKPDDLAVVCFTSGTTGNPKGAMLSHKNIVSNSAGFMKVTEDLMFPSTQDVLISFLPLAHMFERVVECVVLCHGARIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTPVKRWMLDFASKRKEAELRSGIVRNDSFWDKIIFHKVQASLGGKVRLMVTGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSLTIPGDWTAGHVGAPMPCNHVKLVDVEEMNYFASKGEGEVCIKGSNVFQGYLKDDEKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYTRSEPVVQVFVHGESLQAFLVGIVVPDPDNVMNWAKKRKFEGSYEELCKNKDFKNAVLEDLLKLGKEAGLKTFEQVKDIALHSEMFAIENGLLTPTLKAKRPELRKYFKDEIDELYANNKV

Target ORF information:

RefSeq Version	XM_018092309.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092309.1

ORF Insert Sequence:

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGGACTT GATGTTTCCT TCTACTCAAG ATGTTCTGAT TTCTTTTCTG 
CCCTTGGCGC ATATGTTTGA AAGAGTCGTG GAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGT 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acsl1 ( XM_012960022.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815476.1 Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa01904
Clone ID Related Accession (Same CDS sequence)	XM_018092309.1 , NM_001006829.1 , XM_012960022.2
Accession Version	XM_012960022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGGACTT GATGTTTCCT TCTACTCAAG ATGTTCTGAT TTCTTTTCTG 
CCCTTGGCGC ATATGTTTGA AAGAGTCGTG GAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGT 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815476.1
CDS	178..2274
Translation	MQAHEILRNLRIPELGDVRQYVRTLPTNTLMGFGAFAALTTYWYATRPKALKPPCDLAMQSVETQGGEHARRSTILDSDEVITYYYDDVKTIYDIFQRGRRVSNNGPCLGHRKPNQPYEWISYKEVAERAEYVGSALLHRGFKPSPDQFVGIFAQNRPEWTIVELGCYTYSMVAVPLYDTLGAEAITYIINKADLSVVFCDSPEKAKLLLSNVEKGETPVLRTIVLMNPFDNDLVERGKKCGVELVSLKDIEEEGKANREKPKPPKPDDLAVVCFTSGTTGNPKGAMLSHKNIVSNSAGFMKVTEDLMFPSTQDVLISFLPLAHMFERVVECVVLCHGARIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTPVKRWMLDFASKRKEAELRSGIVRNDSFWDKIIFHKVQASLGGKVRLMVTGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSLTIPGDWTAGHVGAPMPCNHVKLVDVEEMNYFASKGEGEVCIKGSNVFQGYLKDDEKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYTRSEPVVQVFVHGESLQAFLVGIVVPDPDNVMNWAKKRKFEGSYEELCKNKDFKNAVLEDLLKLGKEAGLKTFEQVKDIALHSEMFAIENGLLTPTLKAKRPELRKYFKDEIDELYANNKV

Target ORF information:

RefSeq Version	XM_012960022.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960022.2

ORF Insert Sequence:

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGGACTT GATGTTTCCT TCTACTCAAG ATGTTCTGAT TTCTTTTCTG 
CCCTTGGCGC ATATGTTTGA AAGAGTCGTG GAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGT 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acsl1 ( XM_012960017.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815471.1 Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa26700
Clone ID Related Accession (Same CDS sequence)	XM_012960017.2
Accession Version	XM_012960017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGAATAT GCTTACTATG AACGCCAATG ATATACACAT TTCCTATTTG 
CCATTGGCAC ACATGTTTGA AAGACTTGTG CAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGT 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815471.1
CDS	178..2274
Translation	MQAHEILRNLRIPELGDVRQYVRTLPTNTLMGFGAFAALTTYWYATRPKALKPPCDLAMQSVETQGGEHARRSTILDSDEVITYYYDDVKTIYDIFQRGRRVSNNGPCLGHRKPNQPYEWISYKEVAERAEYVGSALLHRGFKPSPDQFVGIFAQNRPEWTIVELGCYTYSMVAVPLYDTLGAEAITYIINKADLSVVFCDSPEKAKLLLSNVEKGETPVLRTIVLMNPFDNDLVERGKKCGVELVSLKDIEEEGKANREKPKPPKPDDLAVVCFTSGTTGNPKGAMLSHKNIVSNSAGFMKVTENMLTMNANDIHISYLPLAHMFERLVQCVVLCHGARIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTPVKRWMLDFASKRKEAELRSGIVRNDSFWDKIIFHKVQASLGGKVRLMVTGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSLTIPGDWTAGHVGAPMPCNHVKLVDVEEMNYFASKGEGEVCIKGSNVFQGYLKDDEKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYTRSEPVVQVFVHGESLQAFLVGIVVPDPDNVMNWAKKRKFEGSYEELCKNKDFKNAVLEDLLKLGKEAGLKTFEQVKDIALHSEMFAIENGLLTPTLKAKRPELRKYFKDEIDELYANNKV

Target ORF information:

RefSeq Version	XM_012960017.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis acyl-CoA synthetase long-chain family member 1 protein (acsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960017.2

ORF Insert Sequence:

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGAATAT GCTTACTATG AACGCCAATG ATATACACAT TTCCTATTTG 
CCATTGGCAC ACATGTTTGA AAGACTTGTG CAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGT 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acsl1 ( XM_031898087.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753947.1 Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa47803
Clone ID Related Accession (Same CDS sequence)	XM_031898087.1 , XM_012960017.3 , XM_031898088.1
Accession Version	XM_031898087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGAATAT GCTTACTATG AACGCCAATG ATATACACAT TTCCTATTTG 
CCATTGGCAC ACATGTTTGA AAGACTTGTG CAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGC 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753947.1
CDS	194..2290
Translation	MQAHEILRNLRIPELGDVRQYVRTLPTNTLMGFGAFAALTTYWYATRPKALKPPCDLAMQSVETQGGEHARRSTILDSDEVITYYYDDVKTIYDIFQRGRRVSNNGPCLGHRKPNQPYEWISYKEVAERAEYVGSALLHRGFKPSPDQFVGIFAQNRPEWTIVELGCYTYSMVAVPLYDTLGAEAITYIINKADLSVVFCDSPEKAKLLLSNVEKGETPVLRTIVLMNPFDNDLVERGKKCGVELVSLKDIEEEGKANREKPKPPKPDDLAVVCFTSGTTGNPKGAMLSHKNIVSNSAGFMKVTENMLTMNANDIHISYLPLAHMFERLVQCVVLCHGARIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTPVKRWMLDFASKRKEAELRSGIVRNDSFWDKIIFHKVQASLGGKVRLMVTGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSLTIPGDWTAGHVGAPMPCNHVKLVDVEEMNYFASKGEGEVCIKGSNVFQGYLKDDEKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYTRSEPVVQVFVHGESLQAFLVGIVVPDPDNVMNWAKKRKFEGSYEELCKNKDFKNAVLEDLLKLGKEAGLKTFEQVKDIALHSEMFAIENGLLTPTLKAKRPELRKYFKDEIDELYANNKV

Target ORF information:

RefSeq Version	XM_031898087.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898087.1

ORF Insert Sequence:

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGAATAT GCTTACTATG AACGCCAATG ATATACACAT TTCCTATTTG 
CCATTGGCAC ACATGTTTGA AAGACTTGTG CAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGC 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acsl1 ( XM_012960017.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815471.1 Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47803
Clone ID Related Accession (Same CDS sequence)	XM_031898087.1 , XM_012960017.3 , XM_031898088.1
Accession Version	XM_012960017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGAATAT GCTTACTATG AACGCCAATG ATATACACAT TTCCTATTTG 
CCATTGGCAC ACATGTTTGA AAGACTTGTG CAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGC 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815471.1
CDS	155..2251
Translation	MQAHEILRNLRIPELGDVRQYVRTLPTNTLMGFGAFAALTTYWYATRPKALKPPCDLAMQSVETQGGEHARRSTILDSDEVITYYYDDVKTIYDIFQRGRRVSNNGPCLGHRKPNQPYEWISYKEVAERAEYVGSALLHRGFKPSPDQFVGIFAQNRPEWTIVELGCYTYSMVAVPLYDTLGAEAITYIINKADLSVVFCDSPEKAKLLLSNVEKGETPVLRTIVLMNPFDNDLVERGKKCGVELVSLKDIEEEGKANREKPKPPKPDDLAVVCFTSGTTGNPKGAMLSHKNIVSNSAGFMKVTENMLTMNANDIHISYLPLAHMFERLVQCVVLCHGARIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTPVKRWMLDFASKRKEAELRSGIVRNDSFWDKIIFHKVQASLGGKVRLMVTGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSLTIPGDWTAGHVGAPMPCNHVKLVDVEEMNYFASKGEGEVCIKGSNVFQGYLKDDEKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYTRSEPVVQVFVHGESLQAFLVGIVVPDPDNVMNWAKKRKFEGSYEELCKNKDFKNAVLEDLLKLGKEAGLKTFEQVKDIALHSEMFAIENGLLTPTLKAKRPELRKYFKDEIDELYANNKV

Target ORF information:

RefSeq Version	XM_012960017.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960017.3

ORF Insert Sequence:

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGAATAT GCTTACTATG AACGCCAATG ATATACACAT TTCCTATTTG 
CCATTGGCAC ACATGTTTGA AAGACTTGTG CAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGC 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acsl1 ( XM_031898088.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753948.1 Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa47803
Clone ID Related Accession (Same CDS sequence)	XM_031898087.1 , XM_012960017.3 , XM_031898088.1
Accession Version	XM_031898088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGAATAT GCTTACTATG AACGCCAATG ATATACACAT TTCCTATTTG 
CCATTGGCAC ACATGTTTGA AAGACTTGTG CAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGC 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753948.1
CDS	133..2229
Translation	MQAHEILRNLRIPELGDVRQYVRTLPTNTLMGFGAFAALTTYWYATRPKALKPPCDLAMQSVETQGGEHARRSTILDSDEVITYYYDDVKTIYDIFQRGRRVSNNGPCLGHRKPNQPYEWISYKEVAERAEYVGSALLHRGFKPSPDQFVGIFAQNRPEWTIVELGCYTYSMVAVPLYDTLGAEAITYIINKADLSVVFCDSPEKAKLLLSNVEKGETPVLRTIVLMNPFDNDLVERGKKCGVELVSLKDIEEEGKANREKPKPPKPDDLAVVCFTSGTTGNPKGAMLSHKNIVSNSAGFMKVTENMLTMNANDIHISYLPLAHMFERLVQCVVLCHGARIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTPVKRWMLDFASKRKEAELRSGIVRNDSFWDKIIFHKVQASLGGKVRLMVTGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSLTIPGDWTAGHVGAPMPCNHVKLVDVEEMNYFASKGEGEVCIKGSNVFQGYLKDDEKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYTRSEPVVQVFVHGESLQAFLVGIVVPDPDNVMNWAKKRKFEGSYEELCKNKDFKNAVLEDLLKLGKEAGLKTFEQVKDIALHSEMFAIENGLLTPTLKAKRPELRKYFKDEIDELYANNKV

Target ORF information:

RefSeq Version	XM_031898088.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898088.1

ORF Insert Sequence:

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGAATAT GCTTACTATG AACGCCAATG ATATACACAT TTCCTATTTG 
CCATTGGCAC ACATGTTTGA AAGACTTGTG CAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGC 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acsl1 ( XM_012960022.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815476.1 Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X2, mRNA.

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CloneID	OXa47804
Clone ID Related Accession (Same CDS sequence)	XM_012960022.3
Accession Version	XM_012960022.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960022.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGGACTT GATGTTTCCT TCTACTCAAG ATGTTCTGAT TTCTTTTCTG 
CCCTTGGCGC ATATGTTTGA AAGAGTCGTG GAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGC 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815476.1
CDS	155..2251
Translation	MQAHEILRNLRIPELGDVRQYVRTLPTNTLMGFGAFAALTTYWYATRPKALKPPCDLAMQSVETQGGEHARRSTILDSDEVITYYYDDVKTIYDIFQRGRRVSNNGPCLGHRKPNQPYEWISYKEVAERAEYVGSALLHRGFKPSPDQFVGIFAQNRPEWTIVELGCYTYSMVAVPLYDTLGAEAITYIINKADLSVVFCDSPEKAKLLLSNVEKGETPVLRTIVLMNPFDNDLVERGKKCGVELVSLKDIEEEGKANREKPKPPKPDDLAVVCFTSGTTGNPKGAMLSHKNIVSNSAGFMKVTEDLMFPSTQDVLISFLPLAHMFERVVECVVLCHGARIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTPVKRWMLDFASKRKEAELRSGIVRNDSFWDKIIFHKVQASLGGKVRLMVTGAAPVSPTVLTFLRAALGCQFYEGYGQTECTAGCSLTIPGDWTAGHVGAPMPCNHVKLVDVEEMNYFASKGEGEVCIKGSNVFQGYLKDDEKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENVYTRSEPVVQVFVHGESLQAFLVGIVVPDPDNVMNWAKKRKFEGSYEELCKNKDFKNAVLEDLLKLGKEAGLKTFEQVKDIALHSEMFAIENGLLTPTLKAKRPELRKYFKDEIDELYANNKV

Target ORF information:

RefSeq Version	XM_012960022.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis acyl-CoA synthetase long chain family member 1 protein (acsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960022.3

ORF Insert Sequence:

ATGCAGGCAC ACGAGATCCT CAGAAACCTC CGGATACCAG AGCTGGGAGA TGTACGGCAG 
TATGTGCGGA CTCTGCCCAC CAACACCTTG ATGGGCTTTG GTGCCTTTGC AGCTCTAACA 
ACATATTGGT ATGCAACAAG ACCCAAGGCT CTGAAACCCC CGTGTGACTT GGCCATGCAG 
TCTGTGGAGA CACAGGGAGG TGAACACGCT CGCAGATCCA CCATTCTGGA TAGTGATGAG 
GTGATCACAT ATTATTATGA TGATGTTAAA ACAATCTATG ACATTTTCCA AAGAGGGAGA 
CGGGTATCAA ATAATGGACC TTGCTTAGGA CATAGGAAAC CAAATCAGCC ATATGAATGG 
ATTTCTTATA AAGAGGTGGC AGAAAGAGCT GAGTATGTTG GGTCTGCGCT GCTGCACAGA 
GGGTTCAAAC CTTCACCGGA TCAATTTGTG GGCATATTTG CACAAAACAG ACCAGAGTGG 
ACAATTGTGG AACTGGGCTG CTACACATAC TCCATGGTGG CTGTGCCCCT GTATGACACT 
CTGGGAGCTG AGGCAATAAC ATACATCATT AACAAAGCTG ATCTCTCAGT GGTGTTTTGC 
GACTCGCCAG AGAAGGCAAA ACTCTTGTTG TCCAATGTTG AAAAGGGAGA AACACCAGTG 
CTCCGCACCA TTGTGCTCAT GAATCCTTTT GACAATGATC TTGTTGAGAG GGGAAAGAAA 
TGTGGTGTTG AATTGGTCAG CTTGAAGGAT ATAGAGGAAG AGGGAAAGGC AAACAGAGAA 
AAACCAAAGC CTCCCAAACC AGATGACCTA GCAGTTGTTT GCTTCACAAG TGGGACAACA 
GGCAATCCGA AGGGGGCAAT GCTATCACAC AAGAACATTG TGAGCAACAG TGCAGGTTTT 
ATGAAAGTAA CAGAGGACTT GATGTTTCCT TCTACTCAAG ATGTTCTGAT TTCTTTTCTG 
CCCTTGGCGC ATATGTTTGA AAGAGTCGTG GAGTGTGTAG TGCTTTGCCA TGGCGCTCGG 
ATTGGATTCT TCCAAGGAGA CATACGGCTG CTCATGGATG ATCTCAAAGT TTTACAACCC 
ACAATATTCC CTGTAGTGCC CCGACTGCTG AACAGAATGT TTGACAGAAT CTTTGGGCAA 
GCCAATACAC CTGTAAAGAG ATGGATGTTG GACTTTGCAT CTAAGAGGAA GGAAGCTGAA 
CTTCGCAGTG GTATTGTCCG AAATGACAGT TTCTGGGACA AAATCATATT TCACAAAGTA 
CAGGCTAGTC TTGGTGGGAA GGTACGACTC ATGGTCACTG GAGCTGCACC TGTCTCTCCG 
ACTGTATTAA CTTTCTTACG GGCAGCACTC GGCTGTCAGT TCTATGAAGG ATATGGACAG 
ACTGAATGCA CAGCAGGATG CTCTCTGACT ATTCCAGGAG ACTGGACAGC AGGTCATGTT 
GGGGCACCAA TGCCCTGTAA TCATGTAAAG TTGGTGGACG TTGAAGAAAT GAATTACTTT 
GCATCAAAGG GAGAGGGTGA GGTTTGTATC AAAGGGTCAA ATGTTTTTCA AGGGTATTTA 
AAAGATGATG AGAAAACTGC AGAGGCCTTG GACAAGGATG GATGGCTGCA TACCGGAGAT 
ATTGGCAAAT GGCTACCAAA CGGCACTCTT AAGATTATAG ACAGAAAAAA ACACATCTTT 
AAGCTGGCAC AGGGGGAATA CATTGCACCA GAGAAAATCG AAAATGTCTA CACTAGAAGC 
GAGCCGGTGG TACAGGTTTT TGTCCATGGA GAGAGTTTAC AGGCATTTTT AGTTGGTATT 
GTGGTGCCAG ATCCTGATAA CGTAATGAAC TGGGCGAAAA AAAGAAAATT TGAGGGCTCT 
TATGAAGAGT TATGCAAAAA CAAGGATTTC AAAAATGCTG TTCTTGAAGA TTTGCTGAAG 
CTTGGCAAAG AAGCAGGGTT AAAAACATTT GAACAGGTAA AAGATATTGC TTTGCACTCT 
GAAATGTTTG CAATTGAGAA CGGCTTGCTG ACGCCTACAC TGAAAGCTAA GCGGCCAGAG 
CTGCGGAAAT ACTTTAAGGA CGAAATTGAT GAACTGTATG CTAATAACAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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acsl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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