adamts8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	adamts8
Entrez Gene ID	100497759
Full Name	ADAM metallopeptidase with thrombospondin type 1 motif 8
General protein information	Preferred Names ADAM metallopeptidase with thrombospondin type 1 motif 8 Names A disintegrin and metalloproteinase with thrombospondin motifs 8
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940639.3	XP_002940685.2	A disintegrin and metalloproteinase with thrombospondin motifs 8
XM_002940639.4	XP_002940685.2	A disintegrin and metalloproteinase with thrombospondin motifs 8

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ADAMTS8	XP_005620353.1
Danio rerio (zebrafish)	adamts8a	XP_698107.4
Xenopus tropicalis (tropical clawed frog)	adamts8	XP_002940685.2
Rattus norvegicus (Norway rat)	Adamts8	XP_003750503.1
Pan troglodytes (chimpanzee)	ADAMTS8	XP_522252.2
Bos taurus (cattle)	ADAMTS8	NP_001179254.1
Mus musculus (house mouse)	Adamts8	NP_038934.2
Gallus gallus (chicken)	ADAMTS8	XP_001232457.3
	ADAMTS8	XP_001112387.2
Danio rerio (zebrafish)	adamts15	XP_001341878.1
Homo sapiens (human)	ADAMTS8	NP_008968.4

Gene Ontology

The following adamts8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the adamts8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa12969	XM_002940639.4 Latest version!	Xenopus tropicalis ADAM metallopeptidase with thrombospondin type 1 motif 8 (adamts8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
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OXa12969	XM_002940639.3	Xenopus tropicalis ADAM metallopeptidase with thrombospondin type 1 motif 8 (adamts8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

adamts8 ( XM_002940639.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940685.2 Xenopus tropicalis ADAM metallopeptidase with thrombospondin type 1 motif 8 (adamts8), mRNA.

Price & Availability↑

CloneID	OXa12969
Clone ID Related Accession (Same CDS sequence)	XM_002940639.3 , XM_002940639.4
Accession Version	XM_002940639.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002940639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAAAT CCTTTGCAAT GGGTTGCATT TACTATCTAT ACTTTGCCTT TTTGCTTCTG 
GTTTGTTCCA ATGCTGCTAG TGTGATGCCA GAAGCAGAAG TGACAGTGCC AGAACTTTTT 
GCAGGATCTG ATGGGAAAAT GACCTTGCGA TTTACTGCAC TAGGCAGAGA TATAGAGCTC 
CATCTTGTTC CTGATGATGC TTTTCTTGGA CCAAGGATGC AGATCAAACA TGTAGGTAGA 
GCTGGTGGGA CACAGACAAG TATGGATGGG TTAAGGACCT GTTTTTATTC CAGTATGGAA 
CCACTGGCTG CTGTTAGTCT ATGCAAAGGT GTTCAGGGTG CCTTTATGCA TGGAAGTGAT 
AGTTATGTTA TCCAGCCCAG AAAAGAATTG AGAGGTCAGA AGAAACTTGG ACAACACTTG 
GTATTTAAGG CACAGAATAA GAACATACCA AGTTGGCATG ACAAATCTTC TGAACTTACA 
GAGGACATAA ACAACCAGGA GTGGGAAAAG CAGGACCTAA AACTGAAAGA CACTCAAAGA 
AACAACCCCA TATTTGGCCA AAAAGAAAGT GTGCTTCTAC AGGATGCAAA AGAAATTGTG 
AGTGGCCACA GCTCTAACTT ATGGACCATC CATGAGATAT CAAAGAGTGA TGTAAAAGGA 
GAGACAGTTC ACCCATTTCG AAACCGAAGA TTTGTGTCTG AAGATCGATT TATAGAGATT 
TTGCTGGTGG CAGACAATAC AATGGTTCAG TTCTATGGAG ATGATTTAGA GCTTCATCTT 
CTGACACTGA TGTCAGTGGC AGCTCGGATC TACAAGCACC CAAGCTTGAG AAACTCTGTG 
ACTCTTGTAG TGGTGACAGT ACTGCTTGTT GAAGATGTTG AATCTGGCCC AGACGTCTCA 
GATAATGGAG GACTGACACT TCGTAGTTTT TGTAACTGGC AACAGAATTT CAATCCATCA 
AGTGATCGCA ATCCTGAACA CTATGATACT GCCTTCCTTC TTACTCGTCA GGATATTTGT 
GGGCATGAGA GCTGTGACAC ACTAGGAGTG GCTGACATTG GCACAGTGTG TGACACGAGC 
AAGAGTTGCT CTGTGATTGA AGATAATGGA CTGCAGGCTG CTTATACCCT TGCCCATGAA 
CTTGGTCATG TCCTTAGCAT ACCCCACGAC AACTCCAAAA ACTGTGAGAG GTATTTTGGA 
GAGCTTGGAA GGCACCACTT GATGGCTTCA CTTTTACAGC TTAATAAGAC TGTACCATGG 
TCCGAATGCA GTGCTATGCA CCTTACTGAG TTTTTTGACA GTGGACATGG AGATTGCCTG 
TTGGATGCAC CATCCAAATC AATAGAGCTT CCTAATGGAC TTCCTGGAAC ATCAGCTCTT 
TATGACTTGG ACAGCCAGTG TCGTCAAGTA TTTGGAAAAG AATTTTCTCA CTGCCCCAAC 
ACATTAGAGA AAGATGTTTG TTCTCAACTC TGGTGTCGAA TTCAGGAAAA AAATGAGTGT 
CATACTAAAA ATGGAAGCTT GCCTTGGGCA GATGGAACTC CCTGTGGTGA TAATCATATG 
TGCTCGGATG GGGTTTGTGT TAAAGAAGAC ATTGTTGTGA GACCAAAGGT ACCAGTGGAT 
GGCAGTTGGG GTAGTTGGGG TCCCTGGGGA GAATGCTCAC GGACCTGTGG AGGGGGTGTG 
CGTTTTTCCT ACAGGGACTG CAATGATCCA GTGCCACAAA ATGGAGGAAA GTACTGCAAG 
GGCCAAAGAG CCATGTATGA ATCATGCAAC ATACAAGAGT GTCCTGTACA TGAGCTGAGC 
TTCAGAGAAG AGCAGTGTAT GAAATACAAT AGCTATAACT ATACAGATGA AGAAGGAACC 
CTTCTGCAAT GGATTCCTAA GTACTCAGGT GTATCATATC GGGATCGTTG TAAACTAGTT 
TGTCAGGCAC GTGACAGGAA TGAATTCAAA GTATTTCAGT CTAAGGTTGT TGATGGAACC 
CTCTGTGAGC CAGATAGTTT GAAAGTATGT GTACAGGGCC AGTGCATCAG TGCAGGATGT 
GACCACATCT TAGGTTCCTC CAAAAAAATG GACAAATGTG GAGTATGCGG AGGCGATGGA 
TCAAGCTGCA GAAAAATTAC TGATTCATTC AATAAGTCAA AGTACGGATA CACTGACATT 
GTCAGCATAC CAGCTGGGGC CACTAATATT GACATCAAGA AGCGTAGTCA CCGTGGTATT 
ATCTATGATG GCAACTATCT AGCAATCAAG AAAGCAGATG GTAGTTATCT GCTAAATGGG 
GATTTTGCTG TGTCATATAT GGAACAAGAC CTACATTTAC GAGGAATTGT CTTGAAATAT 
AGTGGATCTC TGACCACACT AGAAAGAATT AGGAGCTTCT ACCCCCTGCC TGAATCTCTG 
ACAATCCAAC TACTATCAGT CCTTGCAGAG ACCCTACCAC CCAAAGTGAA ATACACTTTC 
TTTATCCCCA AAAATGTTTC ATATGCAAAG ACTAAACCTA AATTATCTCA TCATTCACTC 
AGACCATTGT TAACATCCCA GTGGACTTTT CGGGACTGGT TTCCCTGCTC TAAGAGTTGT 
GGTTTTGGCT GGCAACGACG GGCAGTAGAA TGTCAAGATG TGGAGGGTGG ACCATCAGAC 
CAGTGTCCAC AGGAACTAAG GCCAATAGAT ATTCGACCGT GTGGGGACTT GCCCTGCCCA 
ATTTGGAGAG TAGGAGGTTG GTCACCTTGT TCTCAAAGCT GTGGGGAAGG TGTGCGATCA 
CAAAGGGTCT TTTGTGTAGA CTACACAGGC AGAGAAACAG AGGAGGAGAA GTGTGATCCA 
GGAAAACGTC CTGCAAAGCC AGTTAACTCC TGTATATTGG AGGAATGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940685.2
CDS	754..3624
Translation	MLKSFAMGCIYYLYFAFLLLVCSNAASVMPEAEVTVPELFAGSDGKMTLRFTALGRDIELHLVPDDAFLGPRMQIKHVGRAGGTQTSMDGLRTCFYSSMEPLAAVSLCKGVQGAFMHGSDSYVIQPRKELRGQKKLGQHLVFKAQNKNIPSWHDKSSELTEDINNQEWEKQDLKLKDTQRNNPIFGQKESVLLQDAKEIVSGHSSNLWTIHEISKSDVKGETVHPFRNRRFVSEDRFIEILLVADNTMVQFYGDDLELHLLTLMSVAARIYKHPSLRNSVTLVVVTVLLVEDVESGPDVSDNGGLTLRSFCNWQQNFNPSSDRNPEHYDTAFLLTRQDICGHESCDTLGVADIGTVCDTSKSCSVIEDNGLQAAYTLAHELGHVLSIPHDNSKNCERYFGELGRHHLMASLLQLNKTVPWSECSAMHLTEFFDSGHGDCLLDAPSKSIELPNGLPGTSALYDLDSQCRQVFGKEFSHCPNTLEKDVCSQLWCRIQEKNECHTKNGSLPWADGTPCGDNHMCSDGVCVKEDIVVRPKVPVDGSWGSWGPWGECSRTCGGGVRFSYRDCNDPVPQNGGKYCKGQRAMYESCNIQECPVHELSFREEQCMKYNSYNYTDEEGTLLQWIPKYSGVSYRDRCKLVCQARDRNEFKVFQSKVVDGTLCEPDSLKVCVQGQCISAGCDHILGSSKKMDKCGVCGGDGSSCRKITDSFNKSKYGYTDIVSIPAGATNIDIKKRSHRGIIYDGNYLAIKKADGSYLLNGDFAVSYMEQDLHLRGIVLKYSGSLTTLERIRSFYPLPESLTIQLLSVLAETLPPKVKYTFFIPKNVSYAKTKPKLSHHSLRPLLTSQWTFRDWFPCSKSCGFGWQRRAVECQDVEGGPSDQCPQELRPIDIRPCGDLPCPIWRVGGWSPCSQSCGEGVRSQRVFCVDYTGRETEEEKCDPGKRPAKPVNSCILEEC

Target ORF information:

RefSeq Version	XM_002940639.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADAM metallopeptidase with thrombospondin type 1 motif 8 (adamts8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940639.3

ORF Insert Sequence:

ATGCTAAAAT CCTTTGCAAT GGGTTGCATT TACTATCTAT ACTTTGCCTT TTTGCTTCTG 
GTTTGTTCCA ATGCTGCTAG TGTGATGCCA GAAGCAGAAG TGACAGTGCC AGAACTTTTT 
GCAGGATCTG ATGGGAAAAT GACCTTGCGA TTTACTGCAC TAGGCAGAGA TATAGAGCTC 
CATCTTGTTC CTGATGATGC TTTTCTTGGA CCAAGGATGC AGATCAAACA TGTAGGTAGA 
GCTGGTGGGA CACAGACAAG TATGGATGGG TTAAGGACCT GTTTTTATTC CAGTATGGAA 
CCACTGGCTG CTGTTAGTCT ATGCAAAGGT GTTCAGGGTG CCTTTATGCA TGGAAGTGAT 
AGTTATGTTA TCCAGCCCAG AAAAGAATTG AGAGGTCAGA AGAAACTTGG ACAACACTTG 
GTATTTAAGG CACAGAATAA GAACATACCA AGTTGGCATG ACAAATCTTC TGAACTTACA 
GAGGACATAA ACAACCAGGA GTGGGAAAAG CAGGACCTAA AACTGAAAGA CACTCAAAGA 
AACAACCCCA TATTTGGCCA AAAAGAAAGT GTGCTTCTAC AGGATGCAAA AGAAATTGTG 
AGTGGCCACA GCTCTAACTT ATGGACCATC CATGAGATAT CAAAGAGTGA TGTAAAAGGA 
GAGACAGTTC ACCCATTTCG AAACCGAAGA TTTGTGTCTG AAGATCGATT TATAGAGATT 
TTGCTGGTGG CAGACAATAC AATGGTTCAG TTCTATGGAG ATGATTTAGA GCTTCATCTT 
CTGACACTGA TGTCAGTGGC AGCTCGGATC TACAAGCACC CAAGCTTGAG AAACTCTGTG 
ACTCTTGTAG TGGTGACAGT ACTGCTTGTT GAAGATGTTG AATCTGGCCC AGACGTCTCA 
GATAATGGAG GACTGACACT TCGTAGTTTT TGTAACTGGC AACAGAATTT CAATCCATCA 
AGTGATCGCA ATCCTGAACA CTATGATACT GCCTTCCTTC TTACTCGTCA GGATATTTGT 
GGGCATGAGA GCTGTGACAC ACTAGGAGTG GCTGACATTG GCACAGTGTG TGACACGAGC 
AAGAGTTGCT CTGTGATTGA AGATAATGGA CTGCAGGCTG CTTATACCCT TGCCCATGAA 
CTTGGTCATG TCCTTAGCAT ACCCCACGAC AACTCCAAAA ACTGTGAGAG GTATTTTGGA 
GAGCTTGGAA GGCACCACTT GATGGCTTCA CTTTTACAGC TTAATAAGAC TGTACCATGG 
TCCGAATGCA GTGCTATGCA CCTTACTGAG TTTTTTGACA GTGGACATGG AGATTGCCTG 
TTGGATGCAC CATCCAAATC AATAGAGCTT CCTAATGGAC TTCCTGGAAC ATCAGCTCTT 
TATGACTTGG ACAGCCAGTG TCGTCAAGTA TTTGGAAAAG AATTTTCTCA CTGCCCCAAC 
ACATTAGAGA AAGATGTTTG TTCTCAACTC TGGTGTCGAA TTCAGGAAAA AAATGAGTGT 
CATACTAAAA ATGGAAGCTT GCCTTGGGCA GATGGAACTC CCTGTGGTGA TAATCATATG 
TGCTCGGATG GGGTTTGTGT TAAAGAAGAC ATTGTTGTGA GACCAAAGGT ACCAGTGGAT 
GGCAGTTGGG GTAGTTGGGG TCCCTGGGGA GAATGCTCAC GGACCTGTGG AGGGGGTGTG 
CGTTTTTCCT ACAGGGACTG CAATGATCCA GTGCCACAAA ATGGAGGAAA GTACTGCAAG 
GGCCAAAGAG CCATGTATGA ATCATGCAAC ATACAAGAGT GTCCTGTACA TGAGCTGAGC 
TTCAGAGAAG AGCAGTGTAT GAAATACAAT AGCTATAACT ATACAGATGA AGAAGGAACC 
CTTCTGCAAT GGATTCCTAA GTACTCAGGT GTATCATATC GGGATCGTTG TAAACTAGTT 
TGTCAGGCAC GTGACAGGAA TGAATTCAAA GTATTTCAGT CTAAGGTTGT TGATGGAACC 
CTCTGTGAGC CAGATAGTTT GAAAGTATGT GTACAGGGCC AGTGCATCAG TGCAGGATGT 
GACCACATCT TAGGTTCCTC CAAAAAAATG GACAAATGTG GAGTATGCGG AGGCGATGGA 
TCAAGCTGCA GAAAAATTAC TGATTCATTC AATAAGTCAA AGTACGGATA CACTGACATT 
GTCAGCATAC CAGCTGGGGC CACTAATATT GACATCAAGA AGCGTAGTCA CCGTGGTATT 
ATCTATGATG GCAACTATCT AGCAATCAAG AAAGCAGATG GTAGTTATCT GCTAAATGGG 
GATTTTGCTG TGTCATATAT GGAACAAGAC CTACATTTAC GAGGAATTGT CTTGAAATAT 
AGTGGATCTC TGACCACACT AGAAAGAATT AGGAGCTTCT ACCCCCTGCC TGAATCTCTG 
ACAATCCAAC TACTATCAGT CCTTGCAGAG ACCCTACCAC CCAAAGTGAA ATACACTTTC 
TTTATCCCCA AAAATGTTTC ATATGCAAAG ACTAAACCTA AATTATCTCA TCATTCACTC 
AGACCATTGT TAACATCCCA GTGGACTTTT CGGGACTGGT TTCCCTGCTC TAAGAGTTGT 
GGTTTTGGCT GGCAACGACG GGCAGTAGAA TGTCAAGATG TGGAGGGTGG ACCATCAGAC 
CAGTGTCCAC AGGAACTAAG GCCAATAGAT ATTCGACCGT GTGGGGACTT GCCCTGCCCA 
ATTTGGAGAG TAGGAGGTTG GTCACCTTGT TCTCAAAGCT GTGGGGAAGG TGTGCGATCA 
CAAAGGGTCT TTTGTGTAGA CTACACAGGC AGAGAAACAG AGGAGGAGAA GTGTGATCCA 
GGAAAACGTC CTGCAAAGCC AGTTAACTCC TGTATATTGG AGGAATGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adamts8 ( XM_002940639.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940685.2 Xenopus tropicalis ADAM metallopeptidase with thrombospondin type 1 motif 8 (adamts8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa12969
Clone ID Related Accession (Same CDS sequence)	XM_002940639.3 , XM_002940639.4
Accession Version	XM_002940639.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940639.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAAAT CCTTTGCAAT GGGTTGCATT TACTATCTAT ACTTTGCCTT TTTGCTTCTG 
GTTTGTTCCA ATGCTGCTAG TGTGATGCCA GAAGCAGAAG TGACAGTGCC AGAACTTTTT 
GCAGGATCTG ATGGGAAAAT GACCTTGCGA TTTACTGCAC TAGGCAGAGA TATAGAGCTC 
CATCTTGTTC CTGATGATGC TTTTCTTGGA CCAAGGATGC AGATCAAACA TGTAGGTAGA 
GCTGGTGGGA CACAGACAAG TATGGATGGG TTAAGGACCT GTTTTTATTC CAGTATGGAA 
CCACTGGCTG CTGTTAGTCT ATGCAAAGGT GTTCAGGGTG CCTTTATGCA TGGAAGTGAT 
AGTTATGTTA TCCAGCCCAG AAAAGAATTG AGAGGTCAGA AGAAACTTGG ACAACACTTG 
GTATTTAAGG CACAGAATAA GAACATACCA AGTTGGCATG ACAAATCTTC TGAACTTACA 
GAGGACATAA ACAACCAGGA GTGGGAAAAG CAGGACCTAA AACTGAAAGA CACTCAAAGA 
AACAACCCCA TATTTGGCCA AAAAGAAAGT GTGCTTCTAC AGGATGCAAA AGAAATTGTG 
AGTGGCCACA GCTCTAACTT ATGGACCATC CATGAGATAT CAAAGAGTGA TGTAAAAGGA 
GAGACAGTTC ACCCATTTCG AAACCGAAGA TTTGTGTCTG AAGATCGATT TATAGAGATT 
TTGCTGGTGG CAGACAATAC AATGGTTCAG TTCTATGGAG ATGATTTAGA GCTTCATCTT 
CTGACACTGA TGTCAGTGGC AGCTCGGATC TACAAGCACC CAAGCTTGAG AAACTCTGTG 
ACTCTTGTAG TGGTGACAGT ACTGCTTGTT GAAGATGTTG AATCTGGCCC AGACGTCTCA 
GATAATGGAG GACTGACACT TCGTAGTTTT TGTAACTGGC AACAGAATTT CAATCCATCA 
AGTGATCGCA ATCCTGAACA CTATGATACT GCCTTCCTTC TTACTCGTCA GGATATTTGT 
GGGCATGAGA GCTGTGACAC ACTAGGAGTG GCTGACATTG GCACAGTGTG TGACACGAGC 
AAGAGTTGCT CTGTGATTGA AGATAATGGA CTGCAGGCTG CTTATACCCT TGCCCATGAA 
CTTGGTCATG TCCTTAGCAT ACCCCACGAC AACTCCAAAA ACTGTGAGAG GTATTTTGGA 
GAGCTTGGAA GGCACCACTT GATGGCTTCA CTTTTACAGC TTAATAAGAC TGTACCATGG 
TCCGAATGCA GTGCTATGCA CCTTACTGAG TTTTTTGACA GTGGACATGG AGATTGCCTG 
TTGGATGCAC CATCCAAATC AATAGAGCTT CCTAATGGAC TTCCTGGAAC ATCAGCTCTT 
TATGACTTGG ACAGCCAGTG TCGTCAAGTA TTTGGAAAAG AATTTTCTCA CTGCCCCAAC 
ACATTAGAGA AAGATGTTTG TTCTCAACTC TGGTGTCGAA TTCAGGAAAA AAATGAGTGT 
CATACTAAAA ATGGAAGCTT GCCTTGGGCA GATGGAACTC CCTGTGGTGA TAATCATATG 
TGCTCGGATG GGGTTTGTGT TAAAGAAGAC ATTGTTGTGA GACCAAAGGT ACCAGTGGAT 
GGCAGTTGGG GTAGTTGGGG TCCCTGGGGA GAATGCTCAC GGACCTGTGG AGGGGGTGTG 
CGTTTTTCCT ACAGGGACTG CAATGATCCA GTGCCACAAA ATGGAGGAAA GTACTGCAAG 
GGCCAAAGAG CCATGTATGA ATCATGCAAC ATACAAGAGT GTCCTGTACA TGAGCTGAGC 
TTCAGAGAAG AGCAGTGTAT GAAATACAAT AGCTATAACT ATACAGATGA AGAAGGAACC 
CTTCTGCAAT GGATTCCTAA GTACTCAGGT GTATCATATC GGGATCGTTG TAAACTAGTT 
TGTCAGGCAC GTGACAGGAA TGAATTCAAA GTATTTCAGT CTAAGGTTGT TGATGGAACC 
CTCTGTGAGC CAGATAGTTT GAAAGTATGT GTACAGGGCC AGTGCATCAG TGCAGGATGT 
GACCACATCT TAGGTTCCTC CAAAAAAATG GACAAATGTG GAGTATGCGG AGGCGATGGA 
TCAAGCTGCA GAAAAATTAC TGATTCATTC AATAAGTCAA AGTACGGATA CACTGACATT 
GTCAGCATAC CAGCTGGGGC CACTAATATT GACATCAAGA AGCGTAGTCA CCGTGGTATT 
ATCTATGATG GCAACTATCT AGCAATCAAG AAAGCAGATG GTAGTTATCT GCTAAATGGG 
GATTTTGCTG TGTCATATAT GGAACAAGAC CTACATTTAC GAGGAATTGT CTTGAAATAT 
AGTGGATCTC TGACCACACT AGAAAGAATT AGGAGCTTCT ACCCCCTGCC TGAATCTCTG 
ACAATCCAAC TACTATCAGT CCTTGCAGAG ACCCTACCAC CCAAAGTGAA ATACACTTTC 
TTTATCCCCA AAAATGTTTC ATATGCAAAG ACTAAACCTA AATTATCTCA TCATTCACTC 
AGACCATTGT TAACATCCCA GTGGACTTTT CGGGACTGGT TTCCCTGCTC TAAGAGTTGT 
GGTTTTGGCT GGCAACGACG GGCAGTAGAA TGTCAAGATG TGGAGGGTGG ACCATCAGAC 
CAGTGTCCAC AGGAACTAAG GCCAATAGAT ATTCGACCGT GTGGGGACTT GCCCTGCCCA 
ATTTGGAGAG TAGGAGGTTG GTCACCTTGT TCTCAAAGCT GTGGGGAAGG TGTGCGATCA 
CAAAGGGTCT TTTGTGTAGA CTACACAGGC AGAGAAACAG AGGAGGAGAA GTGTGATCCA 
GGAAAACGTC CTGCAAAGCC AGTTAACTCC TGTATATTGG AGGAATGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940685.2
CDS	2142..5012
Translation	MLKSFAMGCIYYLYFAFLLLVCSNAASVMPEAEVTVPELFAGSDGKMTLRFTALGRDIELHLVPDDAFLGPRMQIKHVGRAGGTQTSMDGLRTCFYSSMEPLAAVSLCKGVQGAFMHGSDSYVIQPRKELRGQKKLGQHLVFKAQNKNIPSWHDKSSELTEDINNQEWEKQDLKLKDTQRNNPIFGQKESVLLQDAKEIVSGHSSNLWTIHEISKSDVKGETVHPFRNRRFVSEDRFIEILLVADNTMVQFYGDDLELHLLTLMSVAARIYKHPSLRNSVTLVVVTVLLVEDVESGPDVSDNGGLTLRSFCNWQQNFNPSSDRNPEHYDTAFLLTRQDICGHESCDTLGVADIGTVCDTSKSCSVIEDNGLQAAYTLAHELGHVLSIPHDNSKNCERYFGELGRHHLMASLLQLNKTVPWSECSAMHLTEFFDSGHGDCLLDAPSKSIELPNGLPGTSALYDLDSQCRQVFGKEFSHCPNTLEKDVCSQLWCRIQEKNECHTKNGSLPWADGTPCGDNHMCSDGVCVKEDIVVRPKVPVDGSWGSWGPWGECSRTCGGGVRFSYRDCNDPVPQNGGKYCKGQRAMYESCNIQECPVHELSFREEQCMKYNSYNYTDEEGTLLQWIPKYSGVSYRDRCKLVCQARDRNEFKVFQSKVVDGTLCEPDSLKVCVQGQCISAGCDHILGSSKKMDKCGVCGGDGSSCRKITDSFNKSKYGYTDIVSIPAGATNIDIKKRSHRGIIYDGNYLAIKKADGSYLLNGDFAVSYMEQDLHLRGIVLKYSGSLTTLERIRSFYPLPESLTIQLLSVLAETLPPKVKYTFFIPKNVSYAKTKPKLSHHSLRPLLTSQWTFRDWFPCSKSCGFGWQRRAVECQDVEGGPSDQCPQELRPIDIRPCGDLPCPIWRVGGWSPCSQSCGEGVRSQRVFCVDYTGRETEEEKCDPGKRPAKPVNSCILEEC

Target ORF information:

RefSeq Version	XM_002940639.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADAM metallopeptidase with thrombospondin type 1 motif 8 (adamts8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940639.4

ORF Insert Sequence:

ATGCTAAAAT CCTTTGCAAT GGGTTGCATT TACTATCTAT ACTTTGCCTT TTTGCTTCTG 
GTTTGTTCCA ATGCTGCTAG TGTGATGCCA GAAGCAGAAG TGACAGTGCC AGAACTTTTT 
GCAGGATCTG ATGGGAAAAT GACCTTGCGA TTTACTGCAC TAGGCAGAGA TATAGAGCTC 
CATCTTGTTC CTGATGATGC TTTTCTTGGA CCAAGGATGC AGATCAAACA TGTAGGTAGA 
GCTGGTGGGA CACAGACAAG TATGGATGGG TTAAGGACCT GTTTTTATTC CAGTATGGAA 
CCACTGGCTG CTGTTAGTCT ATGCAAAGGT GTTCAGGGTG CCTTTATGCA TGGAAGTGAT 
AGTTATGTTA TCCAGCCCAG AAAAGAATTG AGAGGTCAGA AGAAACTTGG ACAACACTTG 
GTATTTAAGG CACAGAATAA GAACATACCA AGTTGGCATG ACAAATCTTC TGAACTTACA 
GAGGACATAA ACAACCAGGA GTGGGAAAAG CAGGACCTAA AACTGAAAGA CACTCAAAGA 
AACAACCCCA TATTTGGCCA AAAAGAAAGT GTGCTTCTAC AGGATGCAAA AGAAATTGTG 
AGTGGCCACA GCTCTAACTT ATGGACCATC CATGAGATAT CAAAGAGTGA TGTAAAAGGA 
GAGACAGTTC ACCCATTTCG AAACCGAAGA TTTGTGTCTG AAGATCGATT TATAGAGATT 
TTGCTGGTGG CAGACAATAC AATGGTTCAG TTCTATGGAG ATGATTTAGA GCTTCATCTT 
CTGACACTGA TGTCAGTGGC AGCTCGGATC TACAAGCACC CAAGCTTGAG AAACTCTGTG 
ACTCTTGTAG TGGTGACAGT ACTGCTTGTT GAAGATGTTG AATCTGGCCC AGACGTCTCA 
GATAATGGAG GACTGACACT TCGTAGTTTT TGTAACTGGC AACAGAATTT CAATCCATCA 
AGTGATCGCA ATCCTGAACA CTATGATACT GCCTTCCTTC TTACTCGTCA GGATATTTGT 
GGGCATGAGA GCTGTGACAC ACTAGGAGTG GCTGACATTG GCACAGTGTG TGACACGAGC 
AAGAGTTGCT CTGTGATTGA AGATAATGGA CTGCAGGCTG CTTATACCCT TGCCCATGAA 
CTTGGTCATG TCCTTAGCAT ACCCCACGAC AACTCCAAAA ACTGTGAGAG GTATTTTGGA 
GAGCTTGGAA GGCACCACTT GATGGCTTCA CTTTTACAGC TTAATAAGAC TGTACCATGG 
TCCGAATGCA GTGCTATGCA CCTTACTGAG TTTTTTGACA GTGGACATGG AGATTGCCTG 
TTGGATGCAC CATCCAAATC AATAGAGCTT CCTAATGGAC TTCCTGGAAC ATCAGCTCTT 
TATGACTTGG ACAGCCAGTG TCGTCAAGTA TTTGGAAAAG AATTTTCTCA CTGCCCCAAC 
ACATTAGAGA AAGATGTTTG TTCTCAACTC TGGTGTCGAA TTCAGGAAAA AAATGAGTGT 
CATACTAAAA ATGGAAGCTT GCCTTGGGCA GATGGAACTC CCTGTGGTGA TAATCATATG 
TGCTCGGATG GGGTTTGTGT TAAAGAAGAC ATTGTTGTGA GACCAAAGGT ACCAGTGGAT 
GGCAGTTGGG GTAGTTGGGG TCCCTGGGGA GAATGCTCAC GGACCTGTGG AGGGGGTGTG 
CGTTTTTCCT ACAGGGACTG CAATGATCCA GTGCCACAAA ATGGAGGAAA GTACTGCAAG 
GGCCAAAGAG CCATGTATGA ATCATGCAAC ATACAAGAGT GTCCTGTACA TGAGCTGAGC 
TTCAGAGAAG AGCAGTGTAT GAAATACAAT AGCTATAACT ATACAGATGA AGAAGGAACC 
CTTCTGCAAT GGATTCCTAA GTACTCAGGT GTATCATATC GGGATCGTTG TAAACTAGTT 
TGTCAGGCAC GTGACAGGAA TGAATTCAAA GTATTTCAGT CTAAGGTTGT TGATGGAACC 
CTCTGTGAGC CAGATAGTTT GAAAGTATGT GTACAGGGCC AGTGCATCAG TGCAGGATGT 
GACCACATCT TAGGTTCCTC CAAAAAAATG GACAAATGTG GAGTATGCGG AGGCGATGGA 
TCAAGCTGCA GAAAAATTAC TGATTCATTC AATAAGTCAA AGTACGGATA CACTGACATT 
GTCAGCATAC CAGCTGGGGC CACTAATATT GACATCAAGA AGCGTAGTCA CCGTGGTATT 
ATCTATGATG GCAACTATCT AGCAATCAAG AAAGCAGATG GTAGTTATCT GCTAAATGGG 
GATTTTGCTG TGTCATATAT GGAACAAGAC CTACATTTAC GAGGAATTGT CTTGAAATAT 
AGTGGATCTC TGACCACACT AGAAAGAATT AGGAGCTTCT ACCCCCTGCC TGAATCTCTG 
ACAATCCAAC TACTATCAGT CCTTGCAGAG ACCCTACCAC CCAAAGTGAA ATACACTTTC 
TTTATCCCCA AAAATGTTTC ATATGCAAAG ACTAAACCTA AATTATCTCA TCATTCACTC 
AGACCATTGT TAACATCCCA GTGGACTTTT CGGGACTGGT TTCCCTGCTC TAAGAGTTGT 
GGTTTTGGCT GGCAACGACG GGCAGTAGAA TGTCAAGATG TGGAGGGTGG ACCATCAGAC 
CAGTGTCCAC AGGAACTAAG GCCAATAGAT ATTCGACCGT GTGGGGACTT GCCCTGCCCA 
ATTTGGAGAG TAGGAGGTTG GTCACCTTGT TCTCAAAGCT GTGGGGAAGG TGTGCGATCA 
CAAAGGGTCT TTTGTGTAGA CTACACAGGC AGAGAAACAG AGGAGGAGAA GTGTGATCCA 
GGAAAACGTC CTGCAAAGCC AGTTAACTCC TGTATATTGG AGGAATGTTG A