lamb1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	lamb1
Entrez Gene ID	100495516
Full Name	laminin subunit beta 1
Synonyms	clm
General protein information	Preferred Names laminin subunit beta 1 Names laminin subunit beta-1 laminin, beta 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933094.3	XP_002933140.2	laminin subunit beta-1
XM_002933094.4	XP_002933140.2	laminin subunit beta-1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LAMB1	NP_001193448.1
Homo sapiens (human)	LAMB1	NP_002282.2
Canis lupus familiaris (dog)	LAMB1	XP_533089.4
Xenopus tropicalis (tropical clawed frog)	lamb1	XP_002933140.2
Pan troglodytes (chimpanzee)	LAMB1	XP_001165667.2
Mus musculus (house mouse)	Lamb1	NP_032508.2
Gallus gallus (chicken)	LAMB1	XP_415943.3
	LAMB1	XP_001090393.2
Rattus norvegicus (Norway rat)	Lamb1	XP_003750185.1
Danio rerio (zebrafish)	lamb1a	NP_775382.1

Gene Ontology

The following lamb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lamb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa54836	XM_002933094.4 Latest version!	Xenopus tropicalis laminin subunit beta 1 (lamb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa07874	XM_002933094.3 Latest version!	Xenopus tropicalis laminin subunit beta 1 (lamb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lamb1 ( XM_002933094.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933140.2 Xenopus tropicalis laminin subunit beta 1 (lamb1), mRNA.

Price & Availability↑

CloneID	OXa54836
Clone ID Related Accession (Same CDS sequence)	XM_002933094.4
Accession Version	XM_002933094.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	laminin subunit beta-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933094.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGT TTCAGATGCC AGGATATTTT CTAGTATTGA TGTTGGGAGC TGCCTATGCC 
CAGAGTCCAG ACTTCAGCCA TGGCTGCGCA GAAGGAAGCT GCTACCCAGC CACAGGAGAT 
CTATTGATTG GTCGATCAGA TCAACTCACT GCATCATCCA CATGTGGCAT CCAGAAACCA 
GAGTCATTTT GTATTGTTAG CCATCTTCAG GAGGAAAAGA AATGCTTTAT ATGTGATTCC 
CTGGATCCTT ACAATGAACA AAATAACACA GAAAGTCACC GCATTGAAAA TGTAGTCACC 
ACATTTGCAC CAAATCGACT TAATTTATGG TGGCAATCAG AAAATGGACT GGAAAATGTT 
ACAATCCAGC TGGACCTTGA AGCGGAATTC CACTTCACTC ATCTCATCAT GACCTTTAAG 
ACATTTCGAC CTGCTGCAAT GCTAATTGAA AGGTCATCTG ATTTTGGAAA AACCTGGAAA 
ATATATAGAT ATTTTGCATA CGACTGTGAG ACCTCCTTTC CTGGCATTTC CACTGGACCG 
ATGAAGAAAG TGGATGATAT AATTTGTGAT TCTCGATATT CAGACATCGA GCCTTCAACA 
GAAGGAGAGG TAATTTATCG TGTTTTGGAT CCTGCCTTCA GAATTGATGA TCCATATAGT 
CTTAGGATTA AAAGTATGCT GAGAATTACA AACCTGAGAA TCAAGTTTTT GAAGCTTCAT 
ACATTGGGGG ACAACCTGCT GGATTCTAGA GTAGAAATAA AAGAAAAATA CTATTATGCA 
ATTTATGACA TGGTAGTTCG CGGAAACTGC TTTTGCTACG GCCATGCAAG TGAATGTGCC 
CCAGTAAAAG GATATTCTGA CAATGTGGAG GGAATGGTGC ATGGGCGATG TGTTTGCAGA 
CACAACACAA AAGGTTTAAA CTGTGAGCAA TGTCTGGATT TCTACCATGA TTTGCCTTGG 
AGACCAGCAG AAGGTCGCAG TAGCAATGCT TGTAAAAAAT GTAACTGTAA CGAACATTCC 
ACACACTGTC ATTTTGATAT GGCTGTTTAT ATGTCTACTG GGAATACCAG TGGAGGGGTG 
TGTGATGACT GCCAACACAA CACAATGGGG CGGAATTGTG AACAGTGCAA ACCATTTTTC 
TACCAGCATC CAGATAAAGA CATACGTGAT CCAAACATCT GTCAACCCTG CAATTGTGAC 
CCAGCTGGAT CCCAGGATGG AGGAATATGT GACCGCTATT CAGATTTTTC CGTGGGCCTT 
ATTGCTGGAC AGTGTCGTTG CAAGTTAAAT GTTGAAGGAG AACGCTGTGA TCAATGCAAA 
GAGGGCTTCT ATGACTTAAA TGCAGAAGAT CCAGAAGGCT GCCAGTTTTG CGGATGTAAC 
CCCCTAGGAA CCATCCCTGG TGGAAACTCT TGTGACTCTA GAAATGGAAA CTGCTTTTGC 
AAACGCTTTG TAACAGGAAG GAAATGTGAT CAGTGCGCGC CTGAATTCTG GGGTTTGAGC 
AATGACTTAG ATGGCTGCCG ACCTTGTGAC TGTGATCATG GGGGATCAGA GGACAATAAC 
TGTTCCCCTG AATCTGGCCA GTGCAGGTGC AGACCACACA TGATTGGACG TCAGTGCAAT 
GAAGTGGAGT CAGGATTTTA TTTTATTTCT TTAGACCATT ACACCTATGA AGCTGAGTAT 
GCAAACTTTG GCCCTGATGT CCGAATTGTT GAGAGACCAC TTCCCCATGA TCGTACCCCT 
TCCTGGACTG GTATTGGGTT TGCAAGAGTT CCAGAAGGAG CATACTTAGA ATTCCACATT 
AATGACATTC CAACTTCCAT GGAGTATGAT CTTTTAATTC GCTATGAGCC TCAACTACCA 
GAGCAGTGGG AAGAGGCAGT TATCACAGTA ATCCGCCCAG GCAAGATTCC CACTAGCAGC 
CGTTGTGGGA ACACTGTCCC AAGTGATGAT AATCAATCAG TGGCATTACC GCCCGGTTCT 
CGTTATATCA CTCTTCCTCG TCCTATTTGC TTTGAGAAGG GACTGAATTA CACAATACGT 
TTGGAGCTGC CTCGGTATTC TGTTAATTCC AATGTGGAAA ATCCATACAC ATTAATTGAT 
TCTATTGTTT TGCTGCCCCA TGTCAAGTCC CTTGACATAT TTAGTATGGG AGGCTCAGGA 
GATGATGTCA CCACCAACAC TGCCTGGCAA ACATTTCAGA GGTACAGATG TCTTGAGAAC 
AGTCAGAGCG TTGTGAAAAC TCCTCTCACC GATGTCTGCA AGAATTTTCT CTTCACCATT 
TCTGCACTTT TACACAAAAG TGCTTTGGAA TGTCAATGTG ACCCACAAGG ATCTTTGAGT 
TCTGTATGCG ATCCAAATGG TGGTCAATGC CAGTGTCGTC CCAACGTCAT TGGAAGAAAC 
TGCAACAAGT GTGCCCCTGG AACCTTTGGC TTTGGACCTA ATGGATGCAA ACCATGTGAG 
TGTGATGTGA GAGGATCTTC TCATCCTTTT TGCCACACTG AGACTGGTCA ATGTCCCTGC 
ATTCATGGAG CATTCGGGCG TCAGTGCGAT GTGTGCTTAC CTGGTTACTG GGGGTTCCCA 
AGCTGCAGGC AGTGCCAGTG TAATGGACAT GCTGAAGACT GTAATGCACT AACTGGAGAG 
TGCACAAACT GCAAGGATCA TTCTGAAGGA TACAGCTGTG AACGGTGTCT TTCAGGTTAT 
TATGGGGACC CCAGACTTGG ATCAGGGGAT CACTGCAGAC CCTGCCCATG TCCTGATGGT 
CCAGGAAGTG AGCGCCAGTT TGCAAGTGGT TGTTACAAAG ATCCTACAAC CTTGCAGGTG 
GTGTGCTCTT GTAATATGGG ATATACAGGA ATAAGATGTG ATGGATGTGC TTCTGGGTAT 
TTTGGAAACC CTGAAGAGAT TGGTGGAGTG TGTCAACCAT GCCACTGTAA TAACAATATA 
GATATGTCTG ACTCACTTGC CTGTCATAAA CAGTCAGGAT TATGCTTGAA GTGTCTGTAT 
CACACCGAAG GGATAAACTG CCAAGAGTGC CAACTAGGTT ATTACGGGAA TGCAATTCAA 
CAGGATTGTA GAAGGTGTGT TTGCAATTAT CTGGGTACAG AACAGGAGAA CTGCAGTGGA 
GAAATAGACT GTCACTGCAA TAGGACAAAT GGGCAGTGTC CATGCTTGCC CAATGTAATT 
GGACAGAGTT GTGATCGTTG TGCAGCAAAC ACCTGGAATT TAGCAAGTGG TACTGGATGT 
GAACCATGTG CATGTGATAC CAATCGGTCT CTAGGGTTAT CTTGTAATGA GTTCACTGGT 
CAGTGCCAGT GCATGCCTGG ATTTGGGGGA CGAGATTGCA GTGAATGCCA GGAACTTTTC 
TGGGGAAATC CAGATATCGA GTGCAGAGCT TGTGATTGTG ACTCTAGAGG CATCCAGACA 
TCACAGTGTA ATCGCGCTAC AGGGCATTGT ATTTGCAAAG AAGGAGTTGA AGGAGTTCGC 
TGTGACAAGT GTGCACGTGG ATATTCTGGA GCATTCCCAT ACTGCACACC TTGTCACAAA 
TGCTTTGCTT TGTGGGATAT CATTATCCAG GAGCTTTCTA ACAGAACAAA GCAGCTTCTA 
AACCGTGCCA ATGACCTGAA GATCTCTGGA GTTATTGGAC CCTACCAGCA GACTGTTGAC 
AAAGTGCAAG AGAAGTTAGA GGAAATCAGA AGCATTCTGG CTCACAATCC AGCAGCACAG 
CCACTCAAAA ATATTGAGAA TCTATTTGAG GAAGCAGAGA AGCTGAAATC TGAAGTTTCA 
CAAAAAGTTG ATGAAGTGGA AATGAAACTA GCAGAAATTA AAATAAATGA CACAGAGAAG 
CTTGATGCTC TTCAGGCAGA TGCTGAAAGC TTTGGAAAAG CATTGAAAGA ACTTGGAGAC 
CAGTTAGAAT TTATGAAAAT ATCTAATGTG CAGGGCGCCA TGGACAGCAT CAACAAATAT 
TTCCAGATGT CTCTAGAGGC AGAAGAGATG GTCAATGCCT CTGTGTCCAA TCCAGATAGC 
ATAATTGAAC ATTCAGCTGC TACCCGGCAG GAAGTGGAAA ATCTAATAAA TGAAAACTTG 
TCCCAGTTTA AGAAAGAGCA AGAGGAGCAG ACTCGACTGC TGGATGAACT TGCTGGCAAT 
GGGCAAAGTT TGGATCTCTC ATCACTTTCT GAAAAGACTT GTGGCAACTC TCCTGGTTTG 
CTCTGTGAAG ATTCTGAATG TGGAGGCCTT AACTGCAAGA CAGACGATGG AAAGAGAAAA 
TGTGGTGGTC CAGGATGTGG GGGTCTGGTA ACTGTAGCCC ATAGTGCCTG GAAAACAGCC 
TCAAGTTTTG ACACAGAAAT TCAGAATGCA CTTGCAGAAG TAGAAGAACT GTCTAAAATG 
GTGGCAGAGT CAAAATTAAG GGCAGAAGAA GCTAAGCAAA GTGCTCAGGA TGTCCTAACC 
AAAGCAAATG CCACCAAGGA AAAAGTTAAT CAAAGCAATG AAGACTTAAG AAATCTCATC 
AAACAGATAA GGGAGTTTTT AATGCAAGAT GGCGCTGATC TGGATAGTAT CGAAGCAGTT 
GCTAGTGAAG TACTAAAAAT GGACATGCCC ACTACCCCTG AACAGCTACA GAAATTGACT 
GAAGAAATCC GCGAACGGGT AGAAAGCCTT TCTGGCGTGG ACACTATCCT CAAGCAGAGT 
GCAGGGGATA TTGCAAGAGC AGAGGCTCTC CTAGATGAAG CCAAAGAATC AAGCAAAAGC 
GCTGCAGATA TTAAAGCAAC AGTTGATGCT GTGAAGGAAG CTTTAATGGA AGCAGGCAAA 
GCTCAAAGTG CTGCTGAAAA AGCCATTAAG AGAGCAGATG ATGATATCAA AGGGACCAAT 
GATCTGCTCA CATCTATTGA ATCTGAAGCA GCTGTGTCTG AAGAGACTTT AAATAACGCC 
ACGAAACGGC TCGTTCAACT AGAAAACAAC ATCGACGATC TAATGAAAAA GTCAGCTGCC 
AACTCTCAGA CAGCAAATCA GGTTGAAAAA TCGGTACATG AAGTGAAAGC AAATGCTAAT 
GGAGCTAAAA AGGTTCTTGA TGGCGAACTT ACCAATAAAT ATAAGACAGT TGAAGATTTG 
ATAGCAAAGA AAGCAGAAGA TTCTGAAGAA GCCAGAAGGA AAGCTCAAGA ACTACAGAAG 
GAAGCCAAAG GGTTGCTAGA ACAAGCAAAC AAAAAACTAG ACACAATCAA AGATTTAGAA 
AAGACATATG AAGGTAACGA AAGGATTCTA GAAGAAAAAG CAAAACAGCT AGTGCAACTG 
GAAGAAACAG TCCGAGCTCT TCTTCAAGCC ATCAGCCAGA AAGCAGCAGT GTATAGCACA 
TGCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933140.2
CDS	158..5506
Translation	MGLFQMPGYFLVLMLGAAYAQSPDFSHGCAEGSCYPATGDLLIGRSDQLTASSTCGIQKPESFCIVSHLQEEKKCFICDSLDPYNEQNNTESHRIENVVTTFAPNRLNLWWQSENGLENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWKIYRYFAYDCETSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIYRVLDPAFRIDDPYSLRIKSMLRITNLRIKFLKLHTLGDNLLDSRVEIKEKYYYAIYDMVVRGNCFCYGHASECAPVKGYSDNVEGMVHGRCVCRHNTKGLNCEQCLDFYHDLPWRPAEGRSSNACKKCNCNEHSTHCHFDMAVYMSTGNTSGGVCDDCQHNTMGRNCEQCKPFFYQHPDKDIRDPNICQPCNCDPAGSQDGGICDRYSDFSVGLIAGQCRCKLNVEGERCDQCKEGFYDLNAEDPEGCQFCGCNPLGTIPGGNSCDSRNGNCFCKRFVTGRKCDQCAPEFWGLSNDLDGCRPCDCDHGGSEDNNCSPESGQCRCRPHMIGRQCNEVESGFYFISLDHYTYEAEYANFGPDVRIVERPLPHDRTPSWTGIGFARVPEGAYLEFHINDIPTSMEYDLLIRYEPQLPEQWEEAVITVIRPGKIPTSSRCGNTVPSDDNQSVALPPGSRYITLPRPICFEKGLNYTIRLELPRYSVNSNVENPYTLIDSIVLLPHVKSLDIFSMGGSGDDVTTNTAWQTFQRYRCLENSQSVVKTPLTDVCKNFLFTISALLHKSALECQCDPQGSLSSVCDPNGGQCQCRPNVIGRNCNKCAPGTFGFGPNGCKPCECDVRGSSHPFCHTETGQCPCIHGAFGRQCDVCLPGYWGFPSCRQCQCNGHAEDCNALTGECTNCKDHSEGYSCERCLSGYYGDPRLGSGDHCRPCPCPDGPGSERQFASGCYKDPTTLQVVCSCNMGYTGIRCDGCASGYFGNPEEIGGVCQPCHCNNNIDMSDSLACHKQSGLCLKCLYHTEGINCQECQLGYYGNAIQQDCRRCVCNYLGTEQENCSGEIDCHCNRTNGQCPCLPNVIGQSCDRCAANTWNLASGTGCEPCACDTNRSLGLSCNEFTGQCQCMPGFGGRDCSECQELFWGNPDIECRACDCDSRGIQTSQCNRATGHCICKEGVEGVRCDKCARGYSGAFPYCTPCHKCFALWDIIIQELSNRTKQLLNRANDLKISGVIGPYQQTVDKVQEKLEEIRSILAHNPAAQPLKNIENLFEEAEKLKSEVSQKVDEVEMKLAEIKINDTEKLDALQADAESFGKALKELGDQLEFMKISNVQGAMDSINKYFQMSLEAEEMVNASVSNPDSIIEHSAATRQEVENLINENLSQFKKEQEEQTRLLDELAGNGQSLDLSSLSEKTCGNSPGLLCEDSECGGLNCKTDDGKRKCGGPGCGGLVTVAHSAWKTASSFDTEIQNALAEVEELSKMVAESKLRAEEAKQSAQDVLTKANATKEKVNQSNEDLRNLIKQIREFLMQDGADLDSIEAVASEVLKMDMPTTPEQLQKLTEEIRERVESLSGVDTILKQSAGDIARAEALLDEAKESSKSAADIKATVDAVKEALMEAGKAQSAAEKAIKRADDDIKGTNDLLTSIESEAAVSEETLNNATKRLVQLENNIDDLMKKSAANSQTANQVEKSVHEVKANANGAKKVLDGELTNKYKTVEDLIAKKAEDSEEARRKAQELQKEAKGLLEQANKKLDTIKDLEKTYEGNERILEEKAKQLVQLEETVRALLQAISQKAAVYSTCF

Target ORF information:

RefSeq Version	XM_002933094.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis laminin subunit beta 1 (lamb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933094.4

ORF Insert Sequence:

ATGGGGCTGT TTCAGATGCC AGGATATTTT CTAGTATTGA TGTTGGGAGC TGCCTATGCC 
CAGAGTCCAG ACTTCAGCCA TGGCTGCGCA GAAGGAAGCT GCTACCCAGC CACAGGAGAT 
CTATTGATTG GTCGATCAGA TCAACTCACT GCATCATCCA CATGTGGCAT CCAGAAACCA 
GAGTCATTTT GTATTGTTAG CCATCTTCAG GAGGAAAAGA AATGCTTTAT ATGTGATTCC 
CTGGATCCTT ACAATGAACA AAATAACACA GAAAGTCACC GCATTGAAAA TGTAGTCACC 
ACATTTGCAC CAAATCGACT TAATTTATGG TGGCAATCAG AAAATGGACT GGAAAATGTT 
ACAATCCAGC TGGACCTTGA AGCGGAATTC CACTTCACTC ATCTCATCAT GACCTTTAAG 
ACATTTCGAC CTGCTGCAAT GCTAATTGAA AGGTCATCTG ATTTTGGAAA AACCTGGAAA 
ATATATAGAT ATTTTGCATA CGACTGTGAG ACCTCCTTTC CTGGCATTTC CACTGGACCG 
ATGAAGAAAG TGGATGATAT AATTTGTGAT TCTCGATATT CAGACATCGA GCCTTCAACA 
GAAGGAGAGG TAATTTATCG TGTTTTGGAT CCTGCCTTCA GAATTGATGA TCCATATAGT 
CTTAGGATTA AAAGTATGCT GAGAATTACA AACCTGAGAA TCAAGTTTTT GAAGCTTCAT 
ACATTGGGGG ACAACCTGCT GGATTCTAGA GTAGAAATAA AAGAAAAATA CTATTATGCA 
ATTTATGACA TGGTAGTTCG CGGAAACTGC TTTTGCTACG GCCATGCAAG TGAATGTGCC 
CCAGTAAAAG GATATTCTGA CAATGTGGAG GGAATGGTGC ATGGGCGATG TGTTTGCAGA 
CACAACACAA AAGGTTTAAA CTGTGAGCAA TGTCTGGATT TCTACCATGA TTTGCCTTGG 
AGACCAGCAG AAGGTCGCAG TAGCAATGCT TGTAAAAAAT GTAACTGTAA CGAACATTCC 
ACACACTGTC ATTTTGATAT GGCTGTTTAT ATGTCTACTG GGAATACCAG TGGAGGGGTG 
TGTGATGACT GCCAACACAA CACAATGGGG CGGAATTGTG AACAGTGCAA ACCATTTTTC 
TACCAGCATC CAGATAAAGA CATACGTGAT CCAAACATCT GTCAACCCTG CAATTGTGAC 
CCAGCTGGAT CCCAGGATGG AGGAATATGT GACCGCTATT CAGATTTTTC CGTGGGCCTT 
ATTGCTGGAC AGTGTCGTTG CAAGTTAAAT GTTGAAGGAG AACGCTGTGA TCAATGCAAA 
GAGGGCTTCT ATGACTTAAA TGCAGAAGAT CCAGAAGGCT GCCAGTTTTG CGGATGTAAC 
CCCCTAGGAA CCATCCCTGG TGGAAACTCT TGTGACTCTA GAAATGGAAA CTGCTTTTGC 
AAACGCTTTG TAACAGGAAG GAAATGTGAT CAGTGCGCGC CTGAATTCTG GGGTTTGAGC 
AATGACTTAG ATGGCTGCCG ACCTTGTGAC TGTGATCATG GGGGATCAGA GGACAATAAC 
TGTTCCCCTG AATCTGGCCA GTGCAGGTGC AGACCACACA TGATTGGACG TCAGTGCAAT 
GAAGTGGAGT CAGGATTTTA TTTTATTTCT TTAGACCATT ACACCTATGA AGCTGAGTAT 
GCAAACTTTG GCCCTGATGT CCGAATTGTT GAGAGACCAC TTCCCCATGA TCGTACCCCT 
TCCTGGACTG GTATTGGGTT TGCAAGAGTT CCAGAAGGAG CATACTTAGA ATTCCACATT 
AATGACATTC CAACTTCCAT GGAGTATGAT CTTTTAATTC GCTATGAGCC TCAACTACCA 
GAGCAGTGGG AAGAGGCAGT TATCACAGTA ATCCGCCCAG GCAAGATTCC CACTAGCAGC 
CGTTGTGGGA ACACTGTCCC AAGTGATGAT AATCAATCAG TGGCATTACC GCCCGGTTCT 
CGTTATATCA CTCTTCCTCG TCCTATTTGC TTTGAGAAGG GACTGAATTA CACAATACGT 
TTGGAGCTGC CTCGGTATTC TGTTAATTCC AATGTGGAAA ATCCATACAC ATTAATTGAT 
TCTATTGTTT TGCTGCCCCA TGTCAAGTCC CTTGACATAT TTAGTATGGG AGGCTCAGGA 
GATGATGTCA CCACCAACAC TGCCTGGCAA ACATTTCAGA GGTACAGATG TCTTGAGAAC 
AGTCAGAGCG TTGTGAAAAC TCCTCTCACC GATGTCTGCA AGAATTTTCT CTTCACCATT 
TCTGCACTTT TACACAAAAG TGCTTTGGAA TGTCAATGTG ACCCACAAGG ATCTTTGAGT 
TCTGTATGCG ATCCAAATGG TGGTCAATGC CAGTGTCGTC CCAACGTCAT TGGAAGAAAC 
TGCAACAAGT GTGCCCCTGG AACCTTTGGC TTTGGACCTA ATGGATGCAA ACCATGTGAG 
TGTGATGTGA GAGGATCTTC TCATCCTTTT TGCCACACTG AGACTGGTCA ATGTCCCTGC 
ATTCATGGAG CATTCGGGCG TCAGTGCGAT GTGTGCTTAC CTGGTTACTG GGGGTTCCCA 
AGCTGCAGGC AGTGCCAGTG TAATGGACAT GCTGAAGACT GTAATGCACT AACTGGAGAG 
TGCACAAACT GCAAGGATCA TTCTGAAGGA TACAGCTGTG AACGGTGTCT TTCAGGTTAT 
TATGGGGACC CCAGACTTGG ATCAGGGGAT CACTGCAGAC CCTGCCCATG TCCTGATGGT 
CCAGGAAGTG AGCGCCAGTT TGCAAGTGGT TGTTACAAAG ATCCTACAAC CTTGCAGGTG 
GTGTGCTCTT GTAATATGGG ATATACAGGA ATAAGATGTG ATGGATGTGC TTCTGGGTAT 
TTTGGAAACC CTGAAGAGAT TGGTGGAGTG TGTCAACCAT GCCACTGTAA TAACAATATA 
GATATGTCTG ACTCACTTGC CTGTCATAAA CAGTCAGGAT TATGCTTGAA GTGTCTGTAT 
CACACCGAAG GGATAAACTG CCAAGAGTGC CAACTAGGTT ATTACGGGAA TGCAATTCAA 
CAGGATTGTA GAAGGTGTGT TTGCAATTAT CTGGGTACAG AACAGGAGAA CTGCAGTGGA 
GAAATAGACT GTCACTGCAA TAGGACAAAT GGGCAGTGTC CATGCTTGCC CAATGTAATT 
GGACAGAGTT GTGATCGTTG TGCAGCAAAC ACCTGGAATT TAGCAAGTGG TACTGGATGT 
GAACCATGTG CATGTGATAC CAATCGGTCT CTAGGGTTAT CTTGTAATGA GTTCACTGGT 
CAGTGCCAGT GCATGCCTGG ATTTGGGGGA CGAGATTGCA GTGAATGCCA GGAACTTTTC 
TGGGGAAATC CAGATATCGA GTGCAGAGCT TGTGATTGTG ACTCTAGAGG CATCCAGACA 
TCACAGTGTA ATCGCGCTAC AGGGCATTGT ATTTGCAAAG AAGGAGTTGA AGGAGTTCGC 
TGTGACAAGT GTGCACGTGG ATATTCTGGA GCATTCCCAT ACTGCACACC TTGTCACAAA 
TGCTTTGCTT TGTGGGATAT CATTATCCAG GAGCTTTCTA ACAGAACAAA GCAGCTTCTA 
AACCGTGCCA ATGACCTGAA GATCTCTGGA GTTATTGGAC CCTACCAGCA GACTGTTGAC 
AAAGTGCAAG AGAAGTTAGA GGAAATCAGA AGCATTCTGG CTCACAATCC AGCAGCACAG 
CCACTCAAAA ATATTGAGAA TCTATTTGAG GAAGCAGAGA AGCTGAAATC TGAAGTTTCA 
CAAAAAGTTG ATGAAGTGGA AATGAAACTA GCAGAAATTA AAATAAATGA CACAGAGAAG 
CTTGATGCTC TTCAGGCAGA TGCTGAAAGC TTTGGAAAAG CATTGAAAGA ACTTGGAGAC 
CAGTTAGAAT TTATGAAAAT ATCTAATGTG CAGGGCGCCA TGGACAGCAT CAACAAATAT 
TTCCAGATGT CTCTAGAGGC AGAAGAGATG GTCAATGCCT CTGTGTCCAA TCCAGATAGC 
ATAATTGAAC ATTCAGCTGC TACCCGGCAG GAAGTGGAAA ATCTAATAAA TGAAAACTTG 
TCCCAGTTTA AGAAAGAGCA AGAGGAGCAG ACTCGACTGC TGGATGAACT TGCTGGCAAT 
GGGCAAAGTT TGGATCTCTC ATCACTTTCT GAAAAGACTT GTGGCAACTC TCCTGGTTTG 
CTCTGTGAAG ATTCTGAATG TGGAGGCCTT AACTGCAAGA CAGACGATGG AAAGAGAAAA 
TGTGGTGGTC CAGGATGTGG GGGTCTGGTA ACTGTAGCCC ATAGTGCCTG GAAAACAGCC 
TCAAGTTTTG ACACAGAAAT TCAGAATGCA CTTGCAGAAG TAGAAGAACT GTCTAAAATG 
GTGGCAGAGT CAAAATTAAG GGCAGAAGAA GCTAAGCAAA GTGCTCAGGA TGTCCTAACC 
AAAGCAAATG CCACCAAGGA AAAAGTTAAT CAAAGCAATG AAGACTTAAG AAATCTCATC 
AAACAGATAA GGGAGTTTTT AATGCAAGAT GGCGCTGATC TGGATAGTAT CGAAGCAGTT 
GCTAGTGAAG TACTAAAAAT GGACATGCCC ACTACCCCTG AACAGCTACA GAAATTGACT 
GAAGAAATCC GCGAACGGGT AGAAAGCCTT TCTGGCGTGG ACACTATCCT CAAGCAGAGT 
GCAGGGGATA TTGCAAGAGC AGAGGCTCTC CTAGATGAAG CCAAAGAATC AAGCAAAAGC 
GCTGCAGATA TTAAAGCAAC AGTTGATGCT GTGAAGGAAG CTTTAATGGA AGCAGGCAAA 
GCTCAAAGTG CTGCTGAAAA AGCCATTAAG AGAGCAGATG ATGATATCAA AGGGACCAAT 
GATCTGCTCA CATCTATTGA ATCTGAAGCA GCTGTGTCTG AAGAGACTTT AAATAACGCC 
ACGAAACGGC TCGTTCAACT AGAAAACAAC ATCGACGATC TAATGAAAAA GTCAGCTGCC 
AACTCTCAGA CAGCAAATCA GGTTGAAAAA TCGGTACATG AAGTGAAAGC AAATGCTAAT 
GGAGCTAAAA AGGTTCTTGA TGGCGAACTT ACCAATAAAT ATAAGACAGT TGAAGATTTG 
ATAGCAAAGA AAGCAGAAGA TTCTGAAGAA GCCAGAAGGA AAGCTCAAGA ACTACAGAAG 
GAAGCCAAAG GGTTGCTAGA ACAAGCAAAC AAAAAACTAG ACACAATCAA AGATTTAGAA 
AAGACATATG AAGGTAACGA AAGGATTCTA GAAGAAAAAG CAAAACAGCT AGTGCAACTG 
GAAGAAACAG TCCGAGCTCT TCTTCAAGCC ATCAGCCAGA AAGCAGCAGT GTATAGCACA 
TGCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lamb1 ( XM_002933094.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933140.2 Xenopus tropicalis laminin subunit beta 1 (lamb1), mRNA.

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CloneID	OXa07874
Clone ID Related Accession (Same CDS sequence)	XM_002933094.3
Accession Version	XM_002933094.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	laminin subunit beta-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGT TTCAGATGCC AGGATATTTT CTAGTATTGA TGTTGGGAGC TGCCTATGCC 
CAGAGTCCAG ACTTCAGCCA TGGCTGCGCA GAAGGAAGCT GCTACCCAGC CACAGGAGAT 
CTATTGATTG GTCGATCAGA TCAACTCACT GCATCATCCA CATGTGGCAT CCAGAAACCA 
GAGTCATTTT GTATTGTTAG CCATCTTCAG GAGGAAAAGA AATGCTTTAT ATGTGATTCC 
CTGGATCCTT ACAATGAACA AAATAACACA GAAAGTCACC GCATTGAAAA TGTAGTCACC 
ACATTTGCAC CAAATCGACT TAATTTATGG TGGCAATCAG AAAATGGACT GGAAAATGTT 
ACAATCCAGC TGGACCTTGA AGCGGAATTC CACTTCACTC ATCTCATCAT GACCTTTAAG 
ACATTTCGAC CTGCTGCAAT GCTAATTGAA AGGTCATCTG ATTTTGGAAA AACCTGGAAA 
ATATATAGAT ATTTTGCATA CGACTGTGAG ACCTCCTTTC CTGGCATTTC CACTGGACCG 
ATGAAGAAAG TGGATGATAT AATTTGTGAT TCTCGATATT CAGACATCGA GCCTTCAACA 
GAAGGAGAGG TAATTTATCG TGTTTTGGAT CCTGCCTTCA GAATTGATGA TCCATATAGT 
CTTAGGATTA AAAGTATGCT GAGAATTACA AACCTGAGAA TCAAGTTTTT GAAGCTTCAT 
ACATTGGGGG ACAACCTGCT GGATTCTAGA GTAGAAATAA AAGAAAAATA CTATTATGCA 
ATTTATGACA TGGTAGTTCG CGGAAACTGC TTTTGCTACG GCCATGCAAG TGAATGTGCC 
CCAGTAAAAG GATATTCTGA CAATGTGGAG GGAATGGTGC ATGGGCGATG TGTTTGCAGA 
CACAACACAA AAGGTTTAAA CTGTGAGCAA TGTCTGGATT TCTACCATGA TTTGCCTTGG 
AGACCAGCAG AAGGTCGCAG TAGCAATGCT TGTAAAAAAT GTAACTGTAA CGAACATTCC 
ACACACTGTC ATTTTGATAT GGCTGTTTAT ATGTCTACTG GGAATACCAG TGGAGGGGTG 
TGTGATGACT GCCAACACAA CACAATGGGG CGGAATTGTG AACAGTGCAA ACCATTTTTC 
TACCAGCATC CAGATAAAGA CATACGTGAT CCAAACATCT GTCAACCCTG CAATTGTGAC 
CCAGCTGGAT CCCAGGATGG AGGAATATGT GACCGCTATT CAGATTTTTC CGTGGGCCTT 
ATTGCTGGAC AGTGTCGTTG CAAGTTAAAT GTTGAAGGAG AACGCTGTGA TCAATGCAAA 
GAGGGCTTCT ATGACTTAAA TGCAGAAGAT CCAGAAGGCT GCCAGTTTTG CGGATGTAAC 
CCCCTAGGAA CCATCCCTGG TGGAAACTCT TGTGACTCTA GAAATGGAAA CTGCTTTTGC 
AAACGCTTTG TAACAGGAAG GAAATGTGAT CAGTGCGCGC CTGAATTCTG GGGTTTGAGC 
AATGACTTAG ATGGCTGCCG ACCTTGTGAC TGTGATCATG GGGGATCAGA GGACAATAAC 
TGTTCCCCTG AATCTGGCCA GTGCAGGTGC AGACCACACA TGATTGGACG TCAGTGCAAT 
GAAGTGGAGT CAGGATTTTA TTTTATTTCT TTAGACCATT ACACCTATGA AGCTGAGTAT 
GCAAACTTTG GCCCTGATGT CCGAATTGTT GAGAGACCAC TTCCCCATGA TCGTACCCCT 
TCCTGGACTG GTATTGGGTT TGCAAGAGTT CCAGAAGGAG CATACTTAGA ATTCCACATT 
AATGACATTC CAACTTCCAT GGAGTATGAT CTTTTAATTC GCTATGAGCC TCAACTACCA 
GAGCAGTGGG AAGAGGCAGT TATCACAGTA ATCCGCCCAG GCAAGATTCC CACTAGCAGC 
CGTTGTGGGA ACACTGTCCC AAGTGATGAT AATCAATCAG TGGCATTACC GCCCGGTTCT 
CGTTATATCA CTCTTCCTCG TCCTATTTGC TTTGAGAAGG GACTGAATTA CACAATACGT 
TTGGAGCTGC CTCGGTATTC TGTTAATTCC AATGTGGAAA ATCCATACAC ATTAATTGAT 
TCTATTGTTT TGCTGCCCCA TGTCAAGTCC CTTGACATAT TTAGTATGGG AGGCTCAGGA 
GATGATGTCA CCACCAACAC TGCCTGGCAA ACATTTCAGA GGTACAGATG TCTTGAGAAC 
AGTCAGAGCG TTGTGAAAAC TCCTCTCACC GATGTCTGCA AGAATTTTCT CTTCACCATT 
TCTGCACTTT TACACAAAAG TGCTTTGGAA TGTCAATGTG ACCCACAAGG ATCTTTGAGT 
TCTGTATGCG ATCCAAATGG TGGTCAATGC CAGTGTCGTC CCAACGTCAT TGGAAGAAAC 
TGCAACAAGT GTGCCCCTGG AACCTTTGGC TTTGGACCTA ATGGATGCAA ACCATGTGAG 
TGTGATGTGA GAGGATCTTC TCATCCTTTT TGCCACACTG AGACTGGTCA ATGTCCCTGC 
ATTCATGGAG CATTCGGGCG TCAGTGCGAT GTGTGCTTAC CTGGTTACTG GGGGTTCCCA 
AGCTGCAGGC AGTGCCAGTG TAATGGACAT GCTGAAGACT GTAATGCACT AACTGGAGAG 
TGCACAAACT GCAAGGATCA TTCTGAAGGA TACAGCTGTG AACGGTGTCT TTCAGGTTAT 
TATGGGGACC CCAGACTTGG ATCAGGGGAT CACTGCAGAC CCTGCCCATG TCCTGATGGT 
CCAGGAAGTG AGCGCCAGTT TGCAAGTGGT TGTTACAAAG ATCCTACAAC CTTGCAGGTG 
GTGTGCTCTT GTAATATGGG ATATACAGGA ATAAGATGTG ATGGATGTGC TTCTGGGTAT 
TTTGGAAACC CTGAAGAGAT TGGTGGAGTG TGTCAACCAT GCCACTGTAA TAACAATATA 
GATATGTCTG ACTCACTTGC CTGTCATAAA CAGTCAGGAT TATGCTTGAA GTGTCTGTAT 
CACACCGAAG GGATAAACTG CCAAGAGTGC CAACTAGGTT ATTACGGGAA TGCAATTCAA 
CAGGATTGTA GAAGGTGTGT TTGCAATTAT CTGGGTACAG AACAGGAGAA CTGCAGTGGA 
GAAATAGACT GTCACTGCAA TAGGACAAAT GGGCAGTGTC CATGCTTGCC CAATGTAATT 
GGACAGAGTT GTGATCGTTG TGCAGCAAAC ACCTGGAATT TAGCAAGTGG TACTGGATGT 
GAACCATGTG CATGTGATAC CAATCGGTCT CTAGGGTTAT CTTGTAATGA GTTCACTGGT 
CAGTGCCAGT GCATGCCTGG ATTTGGGGGA CGAGATTGCA GTGAATGCCA GGAACTTTTC 
TGGGGAAATC CAGATATCGA GTGCAGAGCT TGTGATTGTG ACTCTAGAGG CATCCAGACA 
TCACAGTGTA ATCGCGCTAC AGGGCATTGT ATTTGCAAAG AAGGAGTTGA AGGAGTTCGC 
TGTGACAAGT GTGCACGTGG ATATTCTGGA GCATTCCCAT ACTGCACACC TTGTCACAAA 
TGCTTTGCTT TGTGGGATAT CATTATCCAG GAGCTTTCTA ACAGAACAAA GCAGCTTCTA 
AACCGTGCCA ATGACCTGAA GATCTCTGGA GTTATTGGAC CCTACCAGCA GACTGTTGAC 
AAAGTGCAAG AGAAGTTAGA GGAAATCAGA AGCATTCTGG CTCACAATCC AGCAGCACAG 
CCACTCAAAA ATATTGAGAA TCTATTTGAG GAAGCAGAGA AGCTGAAATC TGAAGTTTCA 
CAAAAAGTTG ATGAAGTGGA AATGAAACTA GCAGAAATTA AAATAAATGA CACAGAGAAG 
CTTGATGCTC TTCAGGCAGA TGCTGAAAGC TTTGGAAAAG CATTGAAAGA ACTTGGAGAC 
CAGTTAGAAT TTATGAAAAT ATCTAATGTG CAGGGCGCCA TGGACAGCAT CAACAAATAT 
TTCCAGATGT CTCTAGAGGC AGAAGAGATG GTCAATGCCT CTGTGTCCAA TCCAGATAGC 
ATAATTGAAC ATTCAGCTGC TACCCGGCAG GAAGTGGAAA ATCTAATAAA TGAAAACTTG 
TCCCAGTTTA AGAAAGAGCA AGAGGAGCAG ACTCGACTGC TGGATGAACT TGCTGGCAAT 
GGGCAAAGTT TGGATCTCTC ATCACTTTCT GAAAAGACTT GTGGCAACTC TCCTGGTTTG 
CTCTGTGAAG ATTCTGAATG TGGAGGCCTT AACTGCAAGA CAGACGATGG AAAGAGAAAA 
TGTGGTGGTC CAGGATGTGG GGGTCTGGTA ACTGTAGCCC ATAGTGCCTG GAAAACAGCC 
TCAAGTTTTG ACACAGAAAT TCAGAATGCA CTTGCAGAAG TAGAAGAACT GTCTAAAATG 
GTGGCAGAGT CAAAATTAAG GGCAGAAGAA GCTAAGCAAA GTGCTCAGGA TGTCCTAACC 
AAAGCAAATG CCACCAAGGA AAAAGTTAAT CAAAGCAATG AAGACTTAAG AAATCTCATC 
AAACAGATAA GGGAGTTTTT AATGCAAGAT GGCGCTGATC TGGATAGTAT CGAAGCAGTT 
GCTAGTGAAG TACTAAAAAT GGACATGCCC ACTACCCCTG AACAGCTACA GAAATTGACT 
GAAGAAATCC GCGAACGGGT AGAAAGCCTT TCTGGCGTGG ACACTATCCT CAAGCAGAGT 
GCAGGGGATA TTGCAAGAGC AGAGGCTCTC CTAGATGAAG CCAAAGAATC AAGCAAAAGC 
GCTGCAGATA TTAAAGCAAC AGTTGATGCT GTGAAGGAAG CTTTAATGGA AGCAGGCAAA 
GCTCAAAGTG CTGCTGAAAA AGCCATTAAG AGAGCAGATG ATGATATCAA AGGGACCAAT 
GATCTGCTCA CATCTATTGA ATCTGAAGCA GCTGTGTCTG AAGAGACTTT AAATAACGCC 
ACGAAACGGC TCGTTCAACT AGAAAACAAC ATCGACGATC TAATGAAAAA GTCAGCTGCC 
AACTCTCAGA CAGCAAATCA GGTTGAAAAA TCGGTACATG AAGTGAAAGC AAATGCTAAT 
GGAGCTAAAA AGGTTCTGGA TGGCGAACTT ACCAATAAAT ATAAGACAGT TGAAGATTTG 
ATAGCAAAGA AAGCAGAAGA TTCTGAAGAA GCCAGAAGGA AAGCTCAAGA ACTACAGAAG 
GAAGCCAAAG GGTTGCTAGA ACAAGCAAAC AAAAAACTAG ACACAATCAA AGATTTAGAA 
AAGACATATG AAGGTAACGA AAGGATTCTA GAAGAAAAAG CAAAACAGCT AGTGCAACTG 
GAAGAAACAG TCCGAGCTCT TCTTCAAGCC ATCAGCCAGA AAGCAGCAGT GTATAGCACA 
TGCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933140.2
CDS	158..5506
Translation	MGLFQMPGYFLVLMLGAAYAQSPDFSHGCAEGSCYPATGDLLIGRSDQLTASSTCGIQKPESFCIVSHLQEEKKCFICDSLDPYNEQNNTESHRIENVVTTFAPNRLNLWWQSENGLENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWKIYRYFAYDCETSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIYRVLDPAFRIDDPYSLRIKSMLRITNLRIKFLKLHTLGDNLLDSRVEIKEKYYYAIYDMVVRGNCFCYGHASECAPVKGYSDNVEGMVHGRCVCRHNTKGLNCEQCLDFYHDLPWRPAEGRSSNACKKCNCNEHSTHCHFDMAVYMSTGNTSGGVCDDCQHNTMGRNCEQCKPFFYQHPDKDIRDPNICQPCNCDPAGSQDGGICDRYSDFSVGLIAGQCRCKLNVEGERCDQCKEGFYDLNAEDPEGCQFCGCNPLGTIPGGNSCDSRNGNCFCKRFVTGRKCDQCAPEFWGLSNDLDGCRPCDCDHGGSEDNNCSPESGQCRCRPHMIGRQCNEVESGFYFISLDHYTYEAEYANFGPDVRIVERPLPHDRTPSWTGIGFARVPEGAYLEFHINDIPTSMEYDLLIRYEPQLPEQWEEAVITVIRPGKIPTSSRCGNTVPSDDNQSVALPPGSRYITLPRPICFEKGLNYTIRLELPRYSVNSNVENPYTLIDSIVLLPHVKSLDIFSMGGSGDDVTTNTAWQTFQRYRCLENSQSVVKTPLTDVCKNFLFTISALLHKSALECQCDPQGSLSSVCDPNGGQCQCRPNVIGRNCNKCAPGTFGFGPNGCKPCECDVRGSSHPFCHTETGQCPCIHGAFGRQCDVCLPGYWGFPSCRQCQCNGHAEDCNALTGECTNCKDHSEGYSCERCLSGYYGDPRLGSGDHCRPCPCPDGPGSERQFASGCYKDPTTLQVVCSCNMGYTGIRCDGCASGYFGNPEEIGGVCQPCHCNNNIDMSDSLACHKQSGLCLKCLYHTEGINCQECQLGYYGNAIQQDCRRCVCNYLGTEQENCSGEIDCHCNRTNGQCPCLPNVIGQSCDRCAANTWNLASGTGCEPCACDTNRSLGLSCNEFTGQCQCMPGFGGRDCSECQELFWGNPDIECRACDCDSRGIQTSQCNRATGHCICKEGVEGVRCDKCARGYSGAFPYCTPCHKCFALWDIIIQELSNRTKQLLNRANDLKISGVIGPYQQTVDKVQEKLEEIRSILAHNPAAQPLKNIENLFEEAEKLKSEVSQKVDEVEMKLAEIKINDTEKLDALQADAESFGKALKELGDQLEFMKISNVQGAMDSINKYFQMSLEAEEMVNASVSNPDSIIEHSAATRQEVENLINENLSQFKKEQEEQTRLLDELAGNGQSLDLSSLSEKTCGNSPGLLCEDSECGGLNCKTDDGKRKCGGPGCGGLVTVAHSAWKTASSFDTEIQNALAEVEELSKMVAESKLRAEEAKQSAQDVLTKANATKEKVNQSNEDLRNLIKQIREFLMQDGADLDSIEAVASEVLKMDMPTTPEQLQKLTEEIRERVESLSGVDTILKQSAGDIARAEALLDEAKESSKSAADIKATVDAVKEALMEAGKAQSAAEKAIKRADDDIKGTNDLLTSIESEAAVSEETLNNATKRLVQLENNIDDLMKKSAANSQTANQVEKSVHEVKANANGAKKVLDGELTNKYKTVEDLIAKKAEDSEEARRKAQELQKEAKGLLEQANKKLDTIKDLEKTYEGNERILEEKAKQLVQLEETVRALLQAISQKAAVYSTCF

Target ORF information:

RefSeq Version	XM_002933094.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis laminin subunit beta 1 (lamb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933094.3

ORF Insert Sequence:

ATGGGGCTGT TTCAGATGCC AGGATATTTT CTAGTATTGA TGTTGGGAGC TGCCTATGCC 
CAGAGTCCAG ACTTCAGCCA TGGCTGCGCA GAAGGAAGCT GCTACCCAGC CACAGGAGAT 
CTATTGATTG GTCGATCAGA TCAACTCACT GCATCATCCA CATGTGGCAT CCAGAAACCA 
GAGTCATTTT GTATTGTTAG CCATCTTCAG GAGGAAAAGA AATGCTTTAT ATGTGATTCC 
CTGGATCCTT ACAATGAACA AAATAACACA GAAAGTCACC GCATTGAAAA TGTAGTCACC 
ACATTTGCAC CAAATCGACT TAATTTATGG TGGCAATCAG AAAATGGACT GGAAAATGTT 
ACAATCCAGC TGGACCTTGA AGCGGAATTC CACTTCACTC ATCTCATCAT GACCTTTAAG 
ACATTTCGAC CTGCTGCAAT GCTAATTGAA AGGTCATCTG ATTTTGGAAA AACCTGGAAA 
ATATATAGAT ATTTTGCATA CGACTGTGAG ACCTCCTTTC CTGGCATTTC CACTGGACCG 
ATGAAGAAAG TGGATGATAT AATTTGTGAT TCTCGATATT CAGACATCGA GCCTTCAACA 
GAAGGAGAGG TAATTTATCG TGTTTTGGAT CCTGCCTTCA GAATTGATGA TCCATATAGT 
CTTAGGATTA AAAGTATGCT GAGAATTACA AACCTGAGAA TCAAGTTTTT GAAGCTTCAT 
ACATTGGGGG ACAACCTGCT GGATTCTAGA GTAGAAATAA AAGAAAAATA CTATTATGCA 
ATTTATGACA TGGTAGTTCG CGGAAACTGC TTTTGCTACG GCCATGCAAG TGAATGTGCC 
CCAGTAAAAG GATATTCTGA CAATGTGGAG GGAATGGTGC ATGGGCGATG TGTTTGCAGA 
CACAACACAA AAGGTTTAAA CTGTGAGCAA TGTCTGGATT TCTACCATGA TTTGCCTTGG 
AGACCAGCAG AAGGTCGCAG TAGCAATGCT TGTAAAAAAT GTAACTGTAA CGAACATTCC 
ACACACTGTC ATTTTGATAT GGCTGTTTAT ATGTCTACTG GGAATACCAG TGGAGGGGTG 
TGTGATGACT GCCAACACAA CACAATGGGG CGGAATTGTG AACAGTGCAA ACCATTTTTC 
TACCAGCATC CAGATAAAGA CATACGTGAT CCAAACATCT GTCAACCCTG CAATTGTGAC 
CCAGCTGGAT CCCAGGATGG AGGAATATGT GACCGCTATT CAGATTTTTC CGTGGGCCTT 
ATTGCTGGAC AGTGTCGTTG CAAGTTAAAT GTTGAAGGAG AACGCTGTGA TCAATGCAAA 
GAGGGCTTCT ATGACTTAAA TGCAGAAGAT CCAGAAGGCT GCCAGTTTTG CGGATGTAAC 
CCCCTAGGAA CCATCCCTGG TGGAAACTCT TGTGACTCTA GAAATGGAAA CTGCTTTTGC 
AAACGCTTTG TAACAGGAAG GAAATGTGAT CAGTGCGCGC CTGAATTCTG GGGTTTGAGC 
AATGACTTAG ATGGCTGCCG ACCTTGTGAC TGTGATCATG GGGGATCAGA GGACAATAAC 
TGTTCCCCTG AATCTGGCCA GTGCAGGTGC AGACCACACA TGATTGGACG TCAGTGCAAT 
GAAGTGGAGT CAGGATTTTA TTTTATTTCT TTAGACCATT ACACCTATGA AGCTGAGTAT 
GCAAACTTTG GCCCTGATGT CCGAATTGTT GAGAGACCAC TTCCCCATGA TCGTACCCCT 
TCCTGGACTG GTATTGGGTT TGCAAGAGTT CCAGAAGGAG CATACTTAGA ATTCCACATT 
AATGACATTC CAACTTCCAT GGAGTATGAT CTTTTAATTC GCTATGAGCC TCAACTACCA 
GAGCAGTGGG AAGAGGCAGT TATCACAGTA ATCCGCCCAG GCAAGATTCC CACTAGCAGC 
CGTTGTGGGA ACACTGTCCC AAGTGATGAT AATCAATCAG TGGCATTACC GCCCGGTTCT 
CGTTATATCA CTCTTCCTCG TCCTATTTGC TTTGAGAAGG GACTGAATTA CACAATACGT 
TTGGAGCTGC CTCGGTATTC TGTTAATTCC AATGTGGAAA ATCCATACAC ATTAATTGAT 
TCTATTGTTT TGCTGCCCCA TGTCAAGTCC CTTGACATAT TTAGTATGGG AGGCTCAGGA 
GATGATGTCA CCACCAACAC TGCCTGGCAA ACATTTCAGA GGTACAGATG TCTTGAGAAC 
AGTCAGAGCG TTGTGAAAAC TCCTCTCACC GATGTCTGCA AGAATTTTCT CTTCACCATT 
TCTGCACTTT TACACAAAAG TGCTTTGGAA TGTCAATGTG ACCCACAAGG ATCTTTGAGT 
TCTGTATGCG ATCCAAATGG TGGTCAATGC CAGTGTCGTC CCAACGTCAT TGGAAGAAAC 
TGCAACAAGT GTGCCCCTGG AACCTTTGGC TTTGGACCTA ATGGATGCAA ACCATGTGAG 
TGTGATGTGA GAGGATCTTC TCATCCTTTT TGCCACACTG AGACTGGTCA ATGTCCCTGC 
ATTCATGGAG CATTCGGGCG TCAGTGCGAT GTGTGCTTAC CTGGTTACTG GGGGTTCCCA 
AGCTGCAGGC AGTGCCAGTG TAATGGACAT GCTGAAGACT GTAATGCACT AACTGGAGAG 
TGCACAAACT GCAAGGATCA TTCTGAAGGA TACAGCTGTG AACGGTGTCT TTCAGGTTAT 
TATGGGGACC CCAGACTTGG ATCAGGGGAT CACTGCAGAC CCTGCCCATG TCCTGATGGT 
CCAGGAAGTG AGCGCCAGTT TGCAAGTGGT TGTTACAAAG ATCCTACAAC CTTGCAGGTG 
GTGTGCTCTT GTAATATGGG ATATACAGGA ATAAGATGTG ATGGATGTGC TTCTGGGTAT 
TTTGGAAACC CTGAAGAGAT TGGTGGAGTG TGTCAACCAT GCCACTGTAA TAACAATATA 
GATATGTCTG ACTCACTTGC CTGTCATAAA CAGTCAGGAT TATGCTTGAA GTGTCTGTAT 
CACACCGAAG GGATAAACTG CCAAGAGTGC CAACTAGGTT ATTACGGGAA TGCAATTCAA 
CAGGATTGTA GAAGGTGTGT TTGCAATTAT CTGGGTACAG AACAGGAGAA CTGCAGTGGA 
GAAATAGACT GTCACTGCAA TAGGACAAAT GGGCAGTGTC CATGCTTGCC CAATGTAATT 
GGACAGAGTT GTGATCGTTG TGCAGCAAAC ACCTGGAATT TAGCAAGTGG TACTGGATGT 
GAACCATGTG CATGTGATAC CAATCGGTCT CTAGGGTTAT CTTGTAATGA GTTCACTGGT 
CAGTGCCAGT GCATGCCTGG ATTTGGGGGA CGAGATTGCA GTGAATGCCA GGAACTTTTC 
TGGGGAAATC CAGATATCGA GTGCAGAGCT TGTGATTGTG ACTCTAGAGG CATCCAGACA 
TCACAGTGTA ATCGCGCTAC AGGGCATTGT ATTTGCAAAG AAGGAGTTGA AGGAGTTCGC 
TGTGACAAGT GTGCACGTGG ATATTCTGGA GCATTCCCAT ACTGCACACC TTGTCACAAA 
TGCTTTGCTT TGTGGGATAT CATTATCCAG GAGCTTTCTA ACAGAACAAA GCAGCTTCTA 
AACCGTGCCA ATGACCTGAA GATCTCTGGA GTTATTGGAC CCTACCAGCA GACTGTTGAC 
AAAGTGCAAG AGAAGTTAGA GGAAATCAGA AGCATTCTGG CTCACAATCC AGCAGCACAG 
CCACTCAAAA ATATTGAGAA TCTATTTGAG GAAGCAGAGA AGCTGAAATC TGAAGTTTCA 
CAAAAAGTTG ATGAAGTGGA AATGAAACTA GCAGAAATTA AAATAAATGA CACAGAGAAG 
CTTGATGCTC TTCAGGCAGA TGCTGAAAGC TTTGGAAAAG CATTGAAAGA ACTTGGAGAC 
CAGTTAGAAT TTATGAAAAT ATCTAATGTG CAGGGCGCCA TGGACAGCAT CAACAAATAT 
TTCCAGATGT CTCTAGAGGC AGAAGAGATG GTCAATGCCT CTGTGTCCAA TCCAGATAGC 
ATAATTGAAC ATTCAGCTGC TACCCGGCAG GAAGTGGAAA ATCTAATAAA TGAAAACTTG 
TCCCAGTTTA AGAAAGAGCA AGAGGAGCAG ACTCGACTGC TGGATGAACT TGCTGGCAAT 
GGGCAAAGTT TGGATCTCTC ATCACTTTCT GAAAAGACTT GTGGCAACTC TCCTGGTTTG 
CTCTGTGAAG ATTCTGAATG TGGAGGCCTT AACTGCAAGA CAGACGATGG AAAGAGAAAA 
TGTGGTGGTC CAGGATGTGG GGGTCTGGTA ACTGTAGCCC ATAGTGCCTG GAAAACAGCC 
TCAAGTTTTG ACACAGAAAT TCAGAATGCA CTTGCAGAAG TAGAAGAACT GTCTAAAATG 
GTGGCAGAGT CAAAATTAAG GGCAGAAGAA GCTAAGCAAA GTGCTCAGGA TGTCCTAACC 
AAAGCAAATG CCACCAAGGA AAAAGTTAAT CAAAGCAATG AAGACTTAAG AAATCTCATC 
AAACAGATAA GGGAGTTTTT AATGCAAGAT GGCGCTGATC TGGATAGTAT CGAAGCAGTT 
GCTAGTGAAG TACTAAAAAT GGACATGCCC ACTACCCCTG AACAGCTACA GAAATTGACT 
GAAGAAATCC GCGAACGGGT AGAAAGCCTT TCTGGCGTGG ACACTATCCT CAAGCAGAGT 
GCAGGGGATA TTGCAAGAGC AGAGGCTCTC CTAGATGAAG CCAAAGAATC AAGCAAAAGC 
GCTGCAGATA TTAAAGCAAC AGTTGATGCT GTGAAGGAAG CTTTAATGGA AGCAGGCAAA 
GCTCAAAGTG CTGCTGAAAA AGCCATTAAG AGAGCAGATG ATGATATCAA AGGGACCAAT 
GATCTGCTCA CATCTATTGA ATCTGAAGCA GCTGTGTCTG AAGAGACTTT AAATAACGCC 
ACGAAACGGC TCGTTCAACT AGAAAACAAC ATCGACGATC TAATGAAAAA GTCAGCTGCC 
AACTCTCAGA CAGCAAATCA GGTTGAAAAA TCGGTACATG AAGTGAAAGC AAATGCTAAT 
GGAGCTAAAA AGGTTCTGGA TGGCGAACTT ACCAATAAAT ATAAGACAGT TGAAGATTTG 
ATAGCAAAGA AAGCAGAAGA TTCTGAAGAA GCCAGAAGGA AAGCTCAAGA ACTACAGAAG 
GAAGCCAAAG GGTTGCTAGA ACAAGCAAAC AAAAAACTAG ACACAATCAA AGATTTAGAA 
AAGACATATG AAGGTAACGA AAGGATTCTA GAAGAAAAAG CAAAACAGCT AGTGCAACTG 
GAAGAAACAG TCCGAGCTCT TCTTCAAGCC ATCAGCCAGA AAGCAGCAGT GTATAGCACA 
TGCTTCTAA