tmem67 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmem67
Entrez Gene ID	100492945
Full Name	transmembrane protein 67
Synonyms	mks3
General protein information	Preferred Names transmembrane protein 67 Names meckelin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934420.3	XP_002934466.2	meckelin
XM_002934420.4	XP_002934466.3	meckelin

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tmem67	XP_700974.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002548	XP_312391.5
Pan troglodytes (chimpanzee)	TMEM67	XP_528307.2
Caenorhabditis elegans (roundworm)	mks-3	NP_495591.2
Homo sapiens (human)	TMEM67	NP_714915.3
Mus musculus (house mouse)	Tmem67	NP_808529.2
Xenopus tropicalis (tropical clawed frog)	tmem67	XP_002934466.2
	TMEM67	XP_002805487.1
Bos taurus (cattle)	TMEM67	NP_001192228.1
Rattus norvegicus (Norway rat)	Tmem67	NP_001101386.3
Canis lupus familiaris (dog)	TMEM67	XP_544178.4
Gallus gallus (chicken)	TMEM67	XP_418334.3
Drosophila melanogaster (fruit fly)	CG15923	NP_001097846.1

Gene Ontology

The following tmem67 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmem67 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa11480	XM_002934420.3 Latest version!	Xenopus tropicalis transmembrane protein 67 (tmem67), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa59887	XM_002934420.4 Latest version!	Xenopus tropicalis transmembrane protein 67 (tmem67), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmem67 ( XM_002934420.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934466.2 Xenopus tropicalis transmembrane protein 67 (tmem67), mRNA.

Price & Availability↑

CloneID	OXa11480
Clone ID Related Accession (Same CDS sequence)	XM_002934420.3
Accession Version	XM_002934420.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	meckelin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002934420.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCA AGAAGCGGCT GAGTGTGGGG CACCAAGGCA CGGCTTTATC TGCGGCTCTA 
GGGCTGTGCT GCGCGGTGCT GCTGGCTGGA TTCTGTGGCC CCGGTCCAGT GCAGAGTCAT 
TCCATCCCCC TGCAGCTTCC CAGCGACTGC GGGCTCCAGG AATACTATGA GCCAAGTAAC 
CTCTCCTGTG TGCGGTGCCA GAACTCCCAG AGACCCAGCC ATTGGGGTAC TTCATGTGTG 
TGCCTTGTAG GATACAAGCG TAATTCCACA GGAGACTGTG AGCGGTGCCC TGAGAGTCTG 
GGTGTAACAG CAGATGGATG GAGTTGTATT AAATGCCCCA ATGGGCTGAA CTCAAATGGG 
AATTGCCTCT GTCCTCCAGG AAACATTTTG GTGGAAAGAG ATGTGAATGG CTCCTTGCTG 
TTAGAAGCAG AATGCATTGA CTGCGATGGG ACTGAACCTT CCTTCACTGC TGCCAACACC 
CTAGGAAACA GGTGTGAACG GTGCCATCAC ACGGTTATCA ACAGGAGTAA CTCATGCAGC 
TGTAACCAGC CAAATATTAT GACTGGAGGC ATATGCTATA ATGCAGCGGG GAGTATCCTG 
TCCAAAGCTC TTCTGACTGT TCATTACAGC CAACTGGGTT TGATATTGCG ATCAGAATGG 
TTTGCCACGC ATTTGCAAGC ATCGGCTGCA GCTTGTCTGA TGTACACTAA TCAAACATCA 
TGCCAAGCTC TGGGGAACAT GTGTGTCATG AACATGCATT CTACAAGTAT TTCCAGTGCT 
GACGCTTGCG GTCTGTTCCA GTACATATTT ACAAGCACAG CAGGTCTAGG AGTTATCCAT 
TCCCTTTCAT ACTGGCGAAC AAATCTGCCA TGGCTCTTCT ATGGTGACCA GCCTGGACTG 
GCTTCCAGGA CTCTTGTGTC CTCTCCAGTT CCCACAAGCT ATACTTTTAA AGAAAGTGAG 
GAGGTAGAAA ATACCAAACT GCAGTTTCTA GCGGCTGTGT ATGACGTTCG AGGGAATTTT 
ATAAAATGGC AATCATTGGC TGGAGGACTA CTGCAGTTGT GTCCTGATAT TGTGGCCAGG 
ATAGATGCTG CTTTCAGCTT TGGAACAACG TATAAACAAG AATGTAGAAT TAGTGTTTCT 
AAGCTCCTGC GTGACCACCC CGAGCCTGTG TTCTATGATG TGTATCTGGC CTACCCAGGG 
AATGAGAAGC AGCAGAATCT CCTTCCTGTG CCAGTCCTGA ACCTGAACCT CCAGTACCAT 
GAAATGTTTG TTAACCAAGG AGACAACATC AACAACTGGC TCTTGACTCG CCGAATCTTC 
CTGGTGGATA CTATTAGTGG GCGAGAAAAT TCTCTGGCCG ATCCTCCAAG GCTCATTAGA 
GTGGCTAGCA AAATCACAAT CAGCATTAGA CTTGTACCAG ACACTTACAA GGGAATAATT 
TACCCACCTC TCATGACTGT GCAATACACA GATGTACAGC TGCAAAATCC AGAGTCCCAA 
ATTGTCAAGG TTTCCTTTTC AGTAAATTAT GAAATGAGAC AGTTGGACTC GTTGAAAAAA 
ACTGATGTGG CGATAGGGGT GCTAGGAGGC CTGGCTGTAC TTTGGTCTCT TCTGAAAACT 
GCAGCTTGGA AGAGAAGAAT TGGGAGCTCA TTTATTGACA TGCAGACCGT GATTCACTTT 
TTAATATTCT ATGCTGGGGC CTTGGCAGAT GTGTTTTTTC TCGTGACAGT TGGCACAGGG 
TTTTATTGGC TCATTTTGTT CAAAGGACAG CAATATGTTT CTGTTCTGTT ACCTGTACCA 
GAAGAAGAAT ATAACTTTGT TTCCTATGTC TGGTGTGCAT TTGCTCTAAA GGCTCTACAG 
CTCCTGTACA AAATGACCTT ACTGCTCACT ATAGACGTCT TTTTCATTGA CTGGGAGAGG 
CCCAAGGCAC GAACTCAGCC TTCTGAAGGC AAGACAAAGA ACAGCGTTGC ACCTGTGAGC 
ATATGGAGGA GCTACTTCAT AGCAAATGAA TGGAATGAAA TTCAGACAGT GAGGAAAATC 
AGTCCCCTCT TTCAAGTGTT TGCGGTTCTC TTCTTTTTAG AGATCGCTGG CTTTAAATAC 
CTAGCACTAA TGGATCCATC TTCTAGCCTT TCAAGAAACT CACAGGACTA CATGGCTCCT 
TGGAGCCGAA TTCTAAGATA CGGAGTGTCC ACTGCGATTT GGCTGGCTAT TGGCTGTTTG 
CAGGTTCTCT TCTTCTCTGT GATTTATGAA AGATATGTGG AAGACAAAAT AAGGCAGTTT 
GTTGATTTAT GTTCAATGAG TAATATTTCC GTGTTTATTT TGAGCCACCG CTGCTACGGA 
TATTACATCC ATGGTCGCTC TGTGCATGGA CATTCAGATA CCAACATGGA GGAGATGAAC 
CTAAATTTAA AGAGGGAAGC GGAAAACCTG TGCAGTCAAA GAGGGTTACA ACCAAACTCA 
GATATTCAGA CTTTCCAGAT TTCTATTTCA TCTAGAGCAA GAACGCACTT TGACCAGATC 
CATGAGAGTT TAACAAAGAG ACATGGCCCT TCAAGACTGT TCCACACATC TCTCTCCACT 
TCTGACCTGA ATACCAGAGC CTACAATAGC ATGAATAAAT TCCTTAGTTC TTTTATTGAT 
CATGCTTTTA AAGAAGTTGA TTACTTTGTG AAAGACAAAA TGTTTTTAGA AAGGCTGCTC 
AGCATGGAGT TCATGGAACC GATGGATAAA AGCATCTTTT ATAATGATGA GAATCATTCA 
TTCACTGATG TTCTTTTTTA TGGAAATGAA TCTACTCTCC TCATATTTGA TACCCTATTC 
TTCTGCGTTG TTGACGTTGC TTCACAAAAC TTTGTTCTGG CTGCCATCCT CACATTCCTA 
CAGCAAGAGT TATTCAGGCT GCTCTGTAAT GCAGTGGGGA AGAAGAACTT GGCCTCTAAA 
ACCCAGATTG ATGAAAGGTT CCTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934466.2
CDS	55..3024
Translation	METKKRLSVGHQGTALSAALGLCCAVLLAGFCGPGPVQSHSIPLQLPSDCGLQEYYEPSNLSCVRCQNSQRPSHWGTSCVCLVGYKRNSTGDCERCPESLGVTADGWSCIKCPNGLNSNGNCLCPPGNILVERDVNGSLLLEAECIDCDGTEPSFTAANTLGNRCERCHHTVINRSNSCSCNQPNIMTGGICYNAAGSILSKALLTVHYSQLGLILRSEWFATHLQASAAACLMYTNQTSCQALGNMCVMNMHSTSISSADACGLFQYIFTSTAGLGVIHSLSYWRTNLPWLFYGDQPGLASRTLVSSPVPTSYTFKESEEVENTKLQFLAAVYDVRGNFIKWQSLAGGLLQLCPDIVARIDAAFSFGTTYKQECRISVSKLLRDHPEPVFYDVYLAYPGNEKQQNLLPVPVLNLNLQYHEMFVNQGDNINNWLLTRRIFLVDTISGRENSLADPPRLIRVASKITISIRLVPDTYKGIIYPPLMTVQYTDVQLQNPESQIVKVSFSVNYEMRQLDSLKKTDVAIGVLGGLAVLWSLLKTAAWKRRIGSSFIDMQTVIHFLIFYAGALADVFFLVTVGTGFYWLILFKGQQYVSVLLPVPEEEYNFVSYVWCAFALKALQLLYKMTLLLTIDVFFIDWERPKARTQPSEGKTKNSVAPVSIWRSYFIANEWNEIQTVRKISPLFQVFAVLFFLEIAGFKYLALMDPSSSLSRNSQDYMAPWSRILRYGVSTAIWLAIGCLQVLFFSVIYERYVEDKIRQFVDLCSMSNISVFILSHRCYGYYIHGRSVHGHSDTNMEEMNLNLKREAENLCSQRGLQPNSDIQTFQISISSRARTHFDQIHESLTKRHGPSRLFHTSLSTSDLNTRAYNSMNKFLSSFIDHAFKEVDYFVKDKMFLERLLSMEFMEPMDKSIFYNDENHSFTDVLFYGNESTLLIFDTLFFCVVDVASQNFVLAAILTFLQQELFRLLCNAVGKKNLASKTQIDERFLI

Target ORF information:

RefSeq Version	XM_002934420.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane protein 67 (tmem67), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934420.3

ORF Insert Sequence:

ATGGAGACCA AGAAGCGGCT GAGTGTGGGG CACCAAGGCA CGGCTTTATC TGCGGCTCTA 
GGGCTGTGCT GCGCGGTGCT GCTGGCTGGA TTCTGTGGCC CCGGTCCAGT GCAGAGTCAT 
TCCATCCCCC TGCAGCTTCC CAGCGACTGC GGGCTCCAGG AATACTATGA GCCAAGTAAC 
CTCTCCTGTG TGCGGTGCCA GAACTCCCAG AGACCCAGCC ATTGGGGTAC TTCATGTGTG 
TGCCTTGTAG GATACAAGCG TAATTCCACA GGAGACTGTG AGCGGTGCCC TGAGAGTCTG 
GGTGTAACAG CAGATGGATG GAGTTGTATT AAATGCCCCA ATGGGCTGAA CTCAAATGGG 
AATTGCCTCT GTCCTCCAGG AAACATTTTG GTGGAAAGAG ATGTGAATGG CTCCTTGCTG 
TTAGAAGCAG AATGCATTGA CTGCGATGGG ACTGAACCTT CCTTCACTGC TGCCAACACC 
CTAGGAAACA GGTGTGAACG GTGCCATCAC ACGGTTATCA ACAGGAGTAA CTCATGCAGC 
TGTAACCAGC CAAATATTAT GACTGGAGGC ATATGCTATA ATGCAGCGGG GAGTATCCTG 
TCCAAAGCTC TTCTGACTGT TCATTACAGC CAACTGGGTT TGATATTGCG ATCAGAATGG 
TTTGCCACGC ATTTGCAAGC ATCGGCTGCA GCTTGTCTGA TGTACACTAA TCAAACATCA 
TGCCAAGCTC TGGGGAACAT GTGTGTCATG AACATGCATT CTACAAGTAT TTCCAGTGCT 
GACGCTTGCG GTCTGTTCCA GTACATATTT ACAAGCACAG CAGGTCTAGG AGTTATCCAT 
TCCCTTTCAT ACTGGCGAAC AAATCTGCCA TGGCTCTTCT ATGGTGACCA GCCTGGACTG 
GCTTCCAGGA CTCTTGTGTC CTCTCCAGTT CCCACAAGCT ATACTTTTAA AGAAAGTGAG 
GAGGTAGAAA ATACCAAACT GCAGTTTCTA GCGGCTGTGT ATGACGTTCG AGGGAATTTT 
ATAAAATGGC AATCATTGGC TGGAGGACTA CTGCAGTTGT GTCCTGATAT TGTGGCCAGG 
ATAGATGCTG CTTTCAGCTT TGGAACAACG TATAAACAAG AATGTAGAAT TAGTGTTTCT 
AAGCTCCTGC GTGACCACCC CGAGCCTGTG TTCTATGATG TGTATCTGGC CTACCCAGGG 
AATGAGAAGC AGCAGAATCT CCTTCCTGTG CCAGTCCTGA ACCTGAACCT CCAGTACCAT 
GAAATGTTTG TTAACCAAGG AGACAACATC AACAACTGGC TCTTGACTCG CCGAATCTTC 
CTGGTGGATA CTATTAGTGG GCGAGAAAAT TCTCTGGCCG ATCCTCCAAG GCTCATTAGA 
GTGGCTAGCA AAATCACAAT CAGCATTAGA CTTGTACCAG ACACTTACAA GGGAATAATT 
TACCCACCTC TCATGACTGT GCAATACACA GATGTACAGC TGCAAAATCC AGAGTCCCAA 
ATTGTCAAGG TTTCCTTTTC AGTAAATTAT GAAATGAGAC AGTTGGACTC GTTGAAAAAA 
ACTGATGTGG CGATAGGGGT GCTAGGAGGC CTGGCTGTAC TTTGGTCTCT TCTGAAAACT 
GCAGCTTGGA AGAGAAGAAT TGGGAGCTCA TTTATTGACA TGCAGACCGT GATTCACTTT 
TTAATATTCT ATGCTGGGGC CTTGGCAGAT GTGTTTTTTC TCGTGACAGT TGGCACAGGG 
TTTTATTGGC TCATTTTGTT CAAAGGACAG CAATATGTTT CTGTTCTGTT ACCTGTACCA 
GAAGAAGAAT ATAACTTTGT TTCCTATGTC TGGTGTGCAT TTGCTCTAAA GGCTCTACAG 
CTCCTGTACA AAATGACCTT ACTGCTCACT ATAGACGTCT TTTTCATTGA CTGGGAGAGG 
CCCAAGGCAC GAACTCAGCC TTCTGAAGGC AAGACAAAGA ACAGCGTTGC ACCTGTGAGC 
ATATGGAGGA GCTACTTCAT AGCAAATGAA TGGAATGAAA TTCAGACAGT GAGGAAAATC 
AGTCCCCTCT TTCAAGTGTT TGCGGTTCTC TTCTTTTTAG AGATCGCTGG CTTTAAATAC 
CTAGCACTAA TGGATCCATC TTCTAGCCTT TCAAGAAACT CACAGGACTA CATGGCTCCT 
TGGAGCCGAA TTCTAAGATA CGGAGTGTCC ACTGCGATTT GGCTGGCTAT TGGCTGTTTG 
CAGGTTCTCT TCTTCTCTGT GATTTATGAA AGATATGTGG AAGACAAAAT AAGGCAGTTT 
GTTGATTTAT GTTCAATGAG TAATATTTCC GTGTTTATTT TGAGCCACCG CTGCTACGGA 
TATTACATCC ATGGTCGCTC TGTGCATGGA CATTCAGATA CCAACATGGA GGAGATGAAC 
CTAAATTTAA AGAGGGAAGC GGAAAACCTG TGCAGTCAAA GAGGGTTACA ACCAAACTCA 
GATATTCAGA CTTTCCAGAT TTCTATTTCA TCTAGAGCAA GAACGCACTT TGACCAGATC 
CATGAGAGTT TAACAAAGAG ACATGGCCCT TCAAGACTGT TCCACACATC TCTCTCCACT 
TCTGACCTGA ATACCAGAGC CTACAATAGC ATGAATAAAT TCCTTAGTTC TTTTATTGAT 
CATGCTTTTA AAGAAGTTGA TTACTTTGTG AAAGACAAAA TGTTTTTAGA AAGGCTGCTC 
AGCATGGAGT TCATGGAACC GATGGATAAA AGCATCTTTT ATAATGATGA GAATCATTCA 
TTCACTGATG TTCTTTTTTA TGGAAATGAA TCTACTCTCC TCATATTTGA TACCCTATTC 
TTCTGCGTTG TTGACGTTGC TTCACAAAAC TTTGTTCTGG CTGCCATCCT CACATTCCTA 
CAGCAAGAGT TATTCAGGCT GCTCTGTAAT GCAGTGGGGA AGAAGAACTT GGCCTCTAAA 
ACCCAGATTG ATGAAAGGTT CCTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem67 ( XM_002934420.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934466.3 Xenopus tropicalis transmembrane protein 67 (tmem67), mRNA.

Price & Availability↑

CloneID	OXa59887
Clone ID Related Accession (Same CDS sequence)	XM_002934420.4
Accession Version	XM_002934420.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	meckelin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934420.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCA AGAAGCGGCT GAGTGTGGGG CACCAAGGCA CGGCTTTATC TGCGGCTCTA 
GGGCTGTGCT GCGCGGTGCT GCTGGCTGGA TTCTGTGGCC CCGGTCCAGT GCAGAGTCAT 
TCCATCCCCC TGCAGCTTCC CAGCGACTGC GGGCTCCAGG AATACTATGA GCCAAGTAAC 
CTCTCCTGTG TGCGGTGCCA GAACTCCCAG AGACCCAGCC ATTGGGGTAC TTCATGTGTG 
TGCCTTGTAG GATACAAGCG TAATTCCACA GGAGACTGTG AGCGGTGCCC TGAGAGTCTG 
GGTGTAACAG CAGATGGATG GAGTTGTATT AAATGCCCCA ATGGGCTGAA CTCAAATGGG 
AATTGCCTCT GTCCTCCAGG AAACATTTTG GTGGAAAGAG ATGTGAATGG CTCCTTGCTG 
TTAGAAGCAG AATGCATTGA CTGCGATGGG ACTGAACCTT CCTTCACTGC TGCCAACACC 
CTAGGAAACA GGTGTGAACG GTGCCATCAC ACGGTTATCA ACAGGAGTAA CTCATGCAGC 
TGTAACCAGC CAAATATTAT GACTGGAGGC ATATGCTATA ATGCAGCGGG GAGTATCCTG 
TCCAAAGCTC TTCTGACTGT TCATTACAGC CAACTGGGTT TGATATTGCG ATCAGAATGG 
TTTGCCACGC ATTTGCAAGC ATCGGCTGCA GCTTGTCTGA TGTACAGTAA TCAAACATCA 
TGCCAAGCTC TGGGGAACAT GTGTGTCATG AACATGCATT CTACAAGTAT TTCCAGTGCT 
GACGCTTGCG GTCTGTTCCA GTACATATTT ACAAGCACAG CAGGTCTAGG AGTTATCCAT 
TCCCTTTCAT ACTGGCGAAC AAATCTGCCA TGGCTCTTCT ATGGTGACCA GCCTGGACTG 
GCTTCCAGGA CTCTTGTGTC CTCTCCAGTT CCCACAAGCT ATACTTTTAA AGAAAGTGAG 
GAGGTAGAAA ATACCAAACT GCAGTTTCTA GCGGCTGTGT ATGACGTTCG AGGGAATTTT 
ATAAAATGGC AATCATTGGC TGGAGGACTA CTGCAGTTGT GTCCTGATAT TGTGGCCAGG 
ATAGATGCTG CTTTCAGCTT TGGAACAACG TATAAACAAG AATGTAGAAT TAGTGTTTCT 
AAGCTCCTGC GTGACCACCC CGAGCCTGTG TTCTATGATG TGTATCTGGC CTACCCAGGG 
AATGAGAAGC AGCAGAATCT CCTTCCTGTG CCAGTCCTGA ACCTGAACCT CCAGTACCAT 
GAAATGTTTG TTAACCAAGG AGACAACATC AACAACTGGC TCTTGACTCG CCGAATCTTC 
CTGGTGGATA CTATTAGTGG GCGAGAAAAT TCTCTGGCCG ATCCTCCAAG GCTCATTAGA 
GTGGCTAGCA AAATCACAAT CAGCATTAGA CTTGTACCAG ACACTTACAA GGGAATAATT 
TACCCACCTC TCATGACTGT GCAATACACA GATGTACAGC TGCAAAATCC AGAGTCCCAA 
ATTGTCAAGG TTTCCTTTTC AGTAAATTAT GAAATGAGAC AGTTGGACTC GTTGAAAAAA 
ACTGATGTGG CGATAGGGGT GCTAGGAGGC CTGGCTGTAC TTTGGTCTCT TCTGAAAACT 
GCAGCTTGGA AGAGAAGAAT TGGGAGCTCA TTTATTGACA TGCAGACCGT GATTCACTTT 
TTAATATTCT ATGCTGGGGC CTTGGCAGAT GTGTTTTTTC TCGTGACAGT TGGCACAGGG 
TTTTATTGGC TCATTTTGTT CAAAGGACAG CAATATGTTT CTGTTCTGTT ACCTGTACCA 
GAAGAAGAAT ATAACTTTGT TTCCTATGTC TGGTGTGCAT TTGCTCTAAA GGCTCTACAG 
CTCCTGTACA AAATGACCTT ACTGCTCACT ATAGACGTCT TTTTCATTGA CTGGGAGAGG 
CCCAAGGCAC GAACTCAGCC TTCTGAAGGC AAGACAAAGA ACAGCGTTGC ACCTGTGAGC 
ATATGGAGGA GCTACTTCAT AGCAAATGAA TGGAATGAAA TTCAGACAGT GAGGAAAATC 
AGTCCCCTCT TTCAAGTGTT TGCGGTTCTC TTCTTTTTAG AGATCGCTGG CTTTAAATAC 
CTAGCACTAA TGGATCCATC TTCTAGCCTT TCAAGAAACT CACAGGACTA CATGGCTCCT 
TGGAGCCGAA TTCTAAGATA CGGAGTGTCC ACTGCGATTT GGCTGGCTAT TGGCTGTTTG 
CAGGTTCTCT TCTTCTCTGT GATTTATGAA AGATATGTGG AAGACAAAAT AAGGCAGTTT 
GTTGATTTAT GTTCAATGAG TAATATTTCC GTGTTTATTT TGAGCCACCG CTGCTACGGA 
TATTACATCC ATGGTCGCTC TGTGCATGGA CATTCAGATA CCAACATGGA GGAGATGAAC 
CTAAATTTAA AGAGGGAAGC GGAAAACCTG TGCAGTCAAA GAGGGTTACA ACCAAACTCA 
GATATTCAGA CTTTCCAGAT TTCTATTTCA TCTAGAGCAA GAACGCACTT TGACCAGATC 
CATGAGAGTT TAACAAAGAG ACATGGCCCT TCAAGACTGT TCCACACATC TCTCTCCACT 
TCTGACCTGA ATACCAGAGC CTACAATAGC ATGAATAAAT TCCTTAGTTC TTTTATTGAT 
CATGCTTTTA AAGAAGTTGA TTACTTTGTG AAAGACAAAA TGTTTTTAGA AAGGCTGCTC 
AGCATGGAGT TCATGGAACC GATGGATAAA AGCATCTTTT ATAATGATGA GAATCATTCA 
TTCACTGATG TTCTTTTTTA TGGAAATGAA TCTACTCTCC TCATATTTGA TACCCTATTC 
TTCTGCGTTG TTGACGTTGC TTCACAAAAC TTTGTTCTGG CTGCCATCCT CACATTCCTA 
CAGCAAGAGT TATTCAGGCT GCTCTGTAAT GCAGTGGGGA AGAAGAACTT GGCCTCTAAA 
ACCCAGATTG ATGAAAGGTT CCTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934466.3
CDS	55..3024
Translation	METKKRLSVGHQGTALSAALGLCCAVLLAGFCGPGPVQSHSIPLQLPSDCGLQEYYEPSNLSCVRCQNSQRPSHWGTSCVCLVGYKRNSTGDCERCPESLGVTADGWSCIKCPNGLNSNGNCLCPPGNILVERDVNGSLLLEAECIDCDGTEPSFTAANTLGNRCERCHHTVINRSNSCSCNQPNIMTGGICYNAAGSILSKALLTVHYSQLGLILRSEWFATHLQASAAACLMYSNQTSCQALGNMCVMNMHSTSISSADACGLFQYIFTSTAGLGVIHSLSYWRTNLPWLFYGDQPGLASRTLVSSPVPTSYTFKESEEVENTKLQFLAAVYDVRGNFIKWQSLAGGLLQLCPDIVARIDAAFSFGTTYKQECRISVSKLLRDHPEPVFYDVYLAYPGNEKQQNLLPVPVLNLNLQYHEMFVNQGDNINNWLLTRRIFLVDTISGRENSLADPPRLIRVASKITISIRLVPDTYKGIIYPPLMTVQYTDVQLQNPESQIVKVSFSVNYEMRQLDSLKKTDVAIGVLGGLAVLWSLLKTAAWKRRIGSSFIDMQTVIHFLIFYAGALADVFFLVTVGTGFYWLILFKGQQYVSVLLPVPEEEYNFVSYVWCAFALKALQLLYKMTLLLTIDVFFIDWERPKARTQPSEGKTKNSVAPVSIWRSYFIANEWNEIQTVRKISPLFQVFAVLFFLEIAGFKYLALMDPSSSLSRNSQDYMAPWSRILRYGVSTAIWLAIGCLQVLFFSVIYERYVEDKIRQFVDLCSMSNISVFILSHRCYGYYIHGRSVHGHSDTNMEEMNLNLKREAENLCSQRGLQPNSDIQTFQISISSRARTHFDQIHESLTKRHGPSRLFHTSLSTSDLNTRAYNSMNKFLSSFIDHAFKEVDYFVKDKMFLERLLSMEFMEPMDKSIFYNDENHSFTDVLFYGNESTLLIFDTLFFCVVDVASQNFVLAAILTFLQQELFRLLCNAVGKKNLASKTQIDERFLI

Target ORF information:

RefSeq Version	XM_002934420.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane protein 67 (tmem67), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934420.4

ORF Insert Sequence:

ATGGAGACCA AGAAGCGGCT GAGTGTGGGG CACCAAGGCA CGGCTTTATC TGCGGCTCTA 
GGGCTGTGCT GCGCGGTGCT GCTGGCTGGA TTCTGTGGCC CCGGTCCAGT GCAGAGTCAT 
TCCATCCCCC TGCAGCTTCC CAGCGACTGC GGGCTCCAGG AATACTATGA GCCAAGTAAC 
CTCTCCTGTG TGCGGTGCCA GAACTCCCAG AGACCCAGCC ATTGGGGTAC TTCATGTGTG 
TGCCTTGTAG GATACAAGCG TAATTCCACA GGAGACTGTG AGCGGTGCCC TGAGAGTCTG 
GGTGTAACAG CAGATGGATG GAGTTGTATT AAATGCCCCA ATGGGCTGAA CTCAAATGGG 
AATTGCCTCT GTCCTCCAGG AAACATTTTG GTGGAAAGAG ATGTGAATGG CTCCTTGCTG 
TTAGAAGCAG AATGCATTGA CTGCGATGGG ACTGAACCTT CCTTCACTGC TGCCAACACC 
CTAGGAAACA GGTGTGAACG GTGCCATCAC ACGGTTATCA ACAGGAGTAA CTCATGCAGC 
TGTAACCAGC CAAATATTAT GACTGGAGGC ATATGCTATA ATGCAGCGGG GAGTATCCTG 
TCCAAAGCTC TTCTGACTGT TCATTACAGC CAACTGGGTT TGATATTGCG ATCAGAATGG 
TTTGCCACGC ATTTGCAAGC ATCGGCTGCA GCTTGTCTGA TGTACAGTAA TCAAACATCA 
TGCCAAGCTC TGGGGAACAT GTGTGTCATG AACATGCATT CTACAAGTAT TTCCAGTGCT 
GACGCTTGCG GTCTGTTCCA GTACATATTT ACAAGCACAG CAGGTCTAGG AGTTATCCAT 
TCCCTTTCAT ACTGGCGAAC AAATCTGCCA TGGCTCTTCT ATGGTGACCA GCCTGGACTG 
GCTTCCAGGA CTCTTGTGTC CTCTCCAGTT CCCACAAGCT ATACTTTTAA AGAAAGTGAG 
GAGGTAGAAA ATACCAAACT GCAGTTTCTA GCGGCTGTGT ATGACGTTCG AGGGAATTTT 
ATAAAATGGC AATCATTGGC TGGAGGACTA CTGCAGTTGT GTCCTGATAT TGTGGCCAGG 
ATAGATGCTG CTTTCAGCTT TGGAACAACG TATAAACAAG AATGTAGAAT TAGTGTTTCT 
AAGCTCCTGC GTGACCACCC CGAGCCTGTG TTCTATGATG TGTATCTGGC CTACCCAGGG 
AATGAGAAGC AGCAGAATCT CCTTCCTGTG CCAGTCCTGA ACCTGAACCT CCAGTACCAT 
GAAATGTTTG TTAACCAAGG AGACAACATC AACAACTGGC TCTTGACTCG CCGAATCTTC 
CTGGTGGATA CTATTAGTGG GCGAGAAAAT TCTCTGGCCG ATCCTCCAAG GCTCATTAGA 
GTGGCTAGCA AAATCACAAT CAGCATTAGA CTTGTACCAG ACACTTACAA GGGAATAATT 
TACCCACCTC TCATGACTGT GCAATACACA GATGTACAGC TGCAAAATCC AGAGTCCCAA 
ATTGTCAAGG TTTCCTTTTC AGTAAATTAT GAAATGAGAC AGTTGGACTC GTTGAAAAAA 
ACTGATGTGG CGATAGGGGT GCTAGGAGGC CTGGCTGTAC TTTGGTCTCT TCTGAAAACT 
GCAGCTTGGA AGAGAAGAAT TGGGAGCTCA TTTATTGACA TGCAGACCGT GATTCACTTT 
TTAATATTCT ATGCTGGGGC CTTGGCAGAT GTGTTTTTTC TCGTGACAGT TGGCACAGGG 
TTTTATTGGC TCATTTTGTT CAAAGGACAG CAATATGTTT CTGTTCTGTT ACCTGTACCA 
GAAGAAGAAT ATAACTTTGT TTCCTATGTC TGGTGTGCAT TTGCTCTAAA GGCTCTACAG 
CTCCTGTACA AAATGACCTT ACTGCTCACT ATAGACGTCT TTTTCATTGA CTGGGAGAGG 
CCCAAGGCAC GAACTCAGCC TTCTGAAGGC AAGACAAAGA ACAGCGTTGC ACCTGTGAGC 
ATATGGAGGA GCTACTTCAT AGCAAATGAA TGGAATGAAA TTCAGACAGT GAGGAAAATC 
AGTCCCCTCT TTCAAGTGTT TGCGGTTCTC TTCTTTTTAG AGATCGCTGG CTTTAAATAC 
CTAGCACTAA TGGATCCATC TTCTAGCCTT TCAAGAAACT CACAGGACTA CATGGCTCCT 
TGGAGCCGAA TTCTAAGATA CGGAGTGTCC ACTGCGATTT GGCTGGCTAT TGGCTGTTTG 
CAGGTTCTCT TCTTCTCTGT GATTTATGAA AGATATGTGG AAGACAAAAT AAGGCAGTTT 
GTTGATTTAT GTTCAATGAG TAATATTTCC GTGTTTATTT TGAGCCACCG CTGCTACGGA 
TATTACATCC ATGGTCGCTC TGTGCATGGA CATTCAGATA CCAACATGGA GGAGATGAAC 
CTAAATTTAA AGAGGGAAGC GGAAAACCTG TGCAGTCAAA GAGGGTTACA ACCAAACTCA 
GATATTCAGA CTTTCCAGAT TTCTATTTCA TCTAGAGCAA GAACGCACTT TGACCAGATC 
CATGAGAGTT TAACAAAGAG ACATGGCCCT TCAAGACTGT TCCACACATC TCTCTCCACT 
TCTGACCTGA ATACCAGAGC CTACAATAGC ATGAATAAAT TCCTTAGTTC TTTTATTGAT 
CATGCTTTTA AAGAAGTTGA TTACTTTGTG AAAGACAAAA TGTTTTTAGA AAGGCTGCTC 
AGCATGGAGT TCATGGAACC GATGGATAAA AGCATCTTTT ATAATGATGA GAATCATTCA 
TTCACTGATG TTCTTTTTTA TGGAAATGAA TCTACTCTCC TCATATTTGA TACCCTATTC 
TTCTGCGTTG TTGACGTTGC TTCACAAAAC TTTGTTCTGG CTGCCATCCT CACATTCCTA 
CAGCAAGAGT TATTCAGGCT GCTCTGTAAT GCAGTGGGGA AGAAGAACTT GGCCTCTAAA 
ACCCAGATTG ATGAAAGGTT CCTGATATGA