itga2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	itga2
Entrez Gene ID	100492714
Full Name	integrin subunit alpha 2
General protein information	Preferred Names integrin subunit alpha 2 Names integrin alpha-2 integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933043.4	XP_002933089.2	integrin alpha-2
XM_031894257.1	XP_031750117.1	integrin alpha-2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ITGA2	NP_001159971.1
Mus musculus (house mouse)	Itga2	NP_032422.2
Gallus gallus (chicken)	ITGA2	XP_003643030.2
Danio rerio (zebrafish)	itga2.2	XP_003199352.2
	ITGA2	XP_001095246.1
Canis lupus familiaris (dog)	ITGA2	XP_005619391.1
Rattus norvegicus (Norway rat)	Itga2	XP_345157.5
Pan troglodytes (chimpanzee)	ITGA2	XP_526928.2
Xenopus tropicalis (tropical clawed frog)	itga2	XP_002933089.2
Homo sapiens (human)	ITGA2	NP_002194.2
Danio rerio (zebrafish)	LOC100333484	XP_003199351.2

Gene Ontology

The following itga2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itga2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa49178	XM_031894257.1 Latest version!	Xenopus tropicalis integrin subunit alpha 2 (itga2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa03394	XM_002933043.4 Latest version!	Xenopus tropicalis integrin subunit alpha 2 (itga2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itga2 ( XM_031894257.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750117.1 Xenopus tropicalis integrin subunit alpha 2 (itga2), mRNA.

Price & Availability↑

CloneID	OXa49178
Clone ID Related Accession (Same CDS sequence)	XM_031894257.1
Accession Version	XM_031894257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin alpha-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAA CAACTTGCAT TCCTTGGCTG CTGCTGGTAA TACTACAAGG AAATCTTTTA 
AAGCACAGTG AAGCATACAA CGTTGGCCTT TCAGGAGCCA AGATATATAG CGGGCCTGCC 
AACACGCAGT TTGGGTATTC TGTTCAGCAG TTTCATAACC AAGATGGAAA TTGGTTGCTT 
GTTAGTTCCC CTTGGAGTGG TTATCCAAAA AATAGGATGG GAGACATTTA CAAGTGCTCA 
GTCAGCTCAT CAACGACCAA ATGTGATAAA ATGAATTTAG CGGATGCGAC TATCATTCCC 
AATGTTACTG AGAAAAGAGA AGAAATGAAT CTTGGATTGA CCCTGTTGAG AAATGGAAAT 
ACAGGAGGAT TTTTGGCCTG TGGACCACTG TGGGCACAGC AGTGTGGTAA CAATTACTAC 
GCAACCGGCG TATGCTCTGA TGTTAGCCCA AGTTTTAAAG TAATTAGAAG TTTTTCACCG 
GCTCTTCAAA CATGTTCATC TTATATTGAC ATTGCAATTG TGTGCGATGG ATCAAACAGC 
ATTTATCCCT GGTACGCTGT TAGAAATTTT TTGGAAAAGT TTGTGCAAGG CTTAGATATA 
GGCCCATCGA GCACTCAAGT AAGCCTCATC CAGTATGGAA ATGACCCCCA AGTGATGTAT 
AAAATGGACA CATATAGAAC CAAAGAAGAA ATGGCCAAGG CAATAACTGG TATTGATCAA 
ATGGGTGGAA CTCAGACAAA CACTTTCAAA GCAATTGATT TTACAAGAAA GTTTGCTTTC 
TCTAAAGAAT ACGGCGGGAG GCCCAATGCC AACAAAGTTA TGGTTGTAAT CACAGATGGG 
GAATCACATG ACAATGCTCT CCTATCAAAA GTGATCGGCG ACTGTGAGAA GGACAATATC 
ATTAGATTTG GCATAGCGGT TTTAGGTTAC TACAACAGGT ACAATATCGA TAGCAAAAAT 
CTGATCAAAG AAATCGAGTC CATCGCCAGC GTTCCGAAGG AGAAATTCTT TTTTAATGTG 
TCTGATGAGG TTGCCTTGTT AGAGAAAGCG GGGACCTTGG GCGAGCGCAT TTTTAGTATT 
GAAGGCACTA CACAAGGAGG TGAGACTTTT CAAATGGAAA TGTCCCAAGT TGGTTTCAGT 
GCACAATATT CAGAAAATCA GGGAACACTG ATGTTGGGAG CGGTCGGCGC CTATGACTGG 
AGCGGCACAG TAGTGCATGA AAAAGCAAAG CAGTTTTCTG TTTTTCCATA TAAAGCATTT 
GAAAAGGTCC TGCATGATCG GAATCAAAGC TCATATCTGG GATATTCTGT CGCTGTTATA 
AATATGAAAA ACTCAGTGAA CTTCGTTGCT GGTGCTCCAA GAACAGACTA CACAGGTCAA 
GTCGTAGTGT ATAATATTAC CAGCCGGGGA AATGTCTCCG TCATTCAGAT CCAAAGAGGA 
GAACAGATTG GTTCGTATTT TGGGAGTGTT TTATGCTCTG TGGATGTCAA CAGAGATTCC 
ATTACTGACG TCTTATTAGT AGGAGCCCCT ACATTCATGA ATGAGTACAA GAAAGAAGAA 
GGCCAAGTGT ACATGTTCTC TATAAGAGAT GGAATTTTAG TTCAGCGTGA ACAGCTGGAA 
GGTCCTAAAA GCCTGGAGAA CACCAGGTTT GGCTCAGCCA TAGCAGAGCT CTCTGATATT 
GACTTGGATG GGTATAATGA TGTCATAGTC GGAGCGCCTT TGGAAAACCA GAACAGCGGA 
GCAATTTATA TTTTTAATGG AAACCAAAAC ACAATAAGAA CCAAATATTC CCAGAAAATT 
TTGGGTTCTT CATTCAACCC AGGGCTGCAG TACTTTGGGA GATCTGTGGA TGGACACCAG 
GATTTAAATG GTGACACAAT AAATGATGTA TCGGTGGGGG CTTATGGAAA AGCTATTCAG 
TTATGGTCTC AGCATATAGC TGATGTGTCA GTGAAGGCGA TATTTATTCC ACAACAAATC 
ATTCTTACAA ACAAGAACTG TGAGGTGACA GTGAGAATAT GCTTCAGCGC CGTATTCAGA 
CCCACTAACT CCCAGAACTC CGTAGCTGTG ACATATAACG CCACCTTGGA TGCAGATCTT 
CTCTCATCAA GAGTGACATC TAGAGGACAA TTTAGGGAGA GCAACGACCG GTTCTTACAG 
AGGAATATTG CCATTGGACC CACAGAAAGC TGTTTCGAAC ACATATTCAA TGTTCAGGAG 
ACTTCCAATA CGGAGAATGC TCTTGCTCTA CGGATTAATG TCATTGCACA GAACCCAGGG 
ACAAGCCCAG TCTTAAACCC TTATTCATCA AGTTCCACAG AATGGTTTAT TCCATTCCTG 
AAAGACTGCG GTGATGACAA AATCTGTATT TCAGACCTCT CTCTGCAGAT TCAGCAGATA 
CCAAATGATA TCAAACTGCC CTACATTGTG AGTATGAAGG ACAGGCGCTT ATTGTTCCAA 
GTAACAGTGA CCAACAAACA GGAGAATGCC TATAACACAA GGCTCAACGC TGTCTTCTCA 
GAGAACTTGT TCTTTGCATC CTCTACAACT CCAAGGGATG GAACGGAAGT GTTATGCCAA 
GTTGGTAATG CACAAAATTC AGTCAGCTGC TTGATTGGTT TTCCTTTTCT TAAGGACGGA 
CAAAAGGTTA TATTCGACAT TTGGTTCGAT TTCAATCTGA ACAATCTGCA GAATAAAGCC 
TTTATTTATT TCCAGACAAT GAGTGACAGC AGAGAAGCTC GGGAAAACGA CAATTCAGTC 
AACATTTCTT TGCCAGTTCA GTATGATGCG GAAATCCACC TTACAAGGGA GGCCAACATA 
AATTTTTATG AAGTCTTTCC TGGTTTAAAT ATCCCATCGG TGGTAAACAA GTTTGATGAT 
ATTGGGCTAG AATTCAATTT CTCAGTAAAA GTTAGCACTG GAACAGTTCC ACTAAAAACA 
GCTTTTCTCT CCATTAATGT GCCACAGTTC ACAAAATCAA ACAATCCTCT GCTGTACATC 
ACTTCAGTCC AAACAGACCA GATGAGTGGT GTTTCTTGCA ATGCGAATAT AAATCCTGAC 
CACATAGGAA GAATTCCATA TGAAACATCA TTCAAAGAAG AGAACCTTAA AAATGAGTTG 
AACTGTAAGA GCGCCCGCTG TGCCGCCATC CAATGTTCCC TGGAAGGCCT GCAGCTCCGA 
GAAGATTATT ATGTAAATGT CTCTACCAGA ATCTGGAACG GAACGTTTGC AGCTGCACAT 
TTCCAGACTA TAGAGTTAGT GGCAAATGCA AAAATTGAAA CAATGGACTC AGAAATATTC 
GTCATCAGCA ACAAAGACGC AATGATCCCC CTGACAATAA CAAAGCCTGG AGAGAAATCA 
GAAGTTCCAA TTGGCATTGT GATTGGAAGT GCCATTGCTG GGCTTGTTCT TTTAGCTGCG 
CTAGTTGCCC TTTTGTGGAA GCTCGGCTTC TTCAAAAGAA AATATCAAAA ACTTCAGAAA 
ACGGAAGAAG AAATAGCAGA GACAACGCAA CTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750117.1
CDS	200..3718
Translation	MARTTCIPWLLLVILQGNLLKHSEAYNVGLSGAKIYSGPANTQFGYSVQQFHNQDGNWLLVSSPWSGYPKNRMGDIYKCSVSSSTTKCDKMNLADATIIPNVTEKREEMNLGLTLLRNGNTGGFLACGPLWAQQCGNNYYATGVCSDVSPSFKVIRSFSPALQTCSSYIDIAIVCDGSNSIYPWYAVRNFLEKFVQGLDIGPSSTQVSLIQYGNDPQVMYKMDTYRTKEEMAKAITGIDQMGGTQTNTFKAIDFTRKFAFSKEYGGRPNANKVMVVITDGESHDNALLSKVIGDCEKDNIIRFGIAVLGYYNRYNIDSKNLIKEIESIASVPKEKFFFNVSDEVALLEKAGTLGERIFSIEGTTQGGETFQMEMSQVGFSAQYSENQGTLMLGAVGAYDWSGTVVHEKAKQFSVFPYKAFEKVLHDRNQSSYLGYSVAVINMKNSVNFVAGAPRTDYTGQVVVYNITSRGNVSVIQIQRGEQIGSYFGSVLCSVDVNRDSITDVLLVGAPTFMNEYKKEEGQVYMFSIRDGILVQREQLEGPKSLENTRFGSAIAELSDIDLDGYNDVIVGAPLENQNSGAIYIFNGNQNTIRTKYSQKILGSSFNPGLQYFGRSVDGHQDLNGDTINDVSVGAYGKAIQLWSQHIADVSVKAIFIPQQIILTNKNCEVTVRICFSAVFRPTNSQNSVAVTYNATLDADLLSSRVTSRGQFRESNDRFLQRNIAIGPTESCFEHIFNVQETSNTENALALRINVIAQNPGTSPVLNPYSSSSTEWFIPFLKDCGDDKICISDLSLQIQQIPNDIKLPYIVSMKDRRLLFQVTVTNKQENAYNTRLNAVFSENLFFASSTTPRDGTEVLCQVGNAQNSVSCLIGFPFLKDGQKVIFDIWFDFNLNNLQNKAFIYFQTMSDSREARENDNSVNISLPVQYDAEIHLTREANINFYEVFPGLNIPSVVNKFDDIGLEFNFSVKVSTGTVPLKTAFLSINVPQFTKSNNPLLYITSVQTDQMSGVSCNANINPDHIGRIPYETSFKEENLKNELNCKSARCAAIQCSLEGLQLREDYYVNVSTRIWNGTFAAAHFQTIELVANAKIETMDSEIFVISNKDAMIPLTITKPGEKSEVPIGIVIGSAIAGLVLLAALVALLWKLGFFKRKYQKLQKTEEEIAETTQLN

Target ORF information:

RefSeq Version	XM_031894257.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit alpha 2 (itga2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894257.1

ORF Insert Sequence:

ATGGCGAGAA CAACTTGCAT TCCTTGGCTG CTGCTGGTAA TACTACAAGG AAATCTTTTA 
AAGCACAGTG AAGCATACAA CGTTGGCCTT TCAGGAGCCA AGATATATAG CGGGCCTGCC 
AACACGCAGT TTGGGTATTC TGTTCAGCAG TTTCATAACC AAGATGGAAA TTGGTTGCTT 
GTTAGTTCCC CTTGGAGTGG TTATCCAAAA AATAGGATGG GAGACATTTA CAAGTGCTCA 
GTCAGCTCAT CAACGACCAA ATGTGATAAA ATGAATTTAG CGGATGCGAC TATCATTCCC 
AATGTTACTG AGAAAAGAGA AGAAATGAAT CTTGGATTGA CCCTGTTGAG AAATGGAAAT 
ACAGGAGGAT TTTTGGCCTG TGGACCACTG TGGGCACAGC AGTGTGGTAA CAATTACTAC 
GCAACCGGCG TATGCTCTGA TGTTAGCCCA AGTTTTAAAG TAATTAGAAG TTTTTCACCG 
GCTCTTCAAA CATGTTCATC TTATATTGAC ATTGCAATTG TGTGCGATGG ATCAAACAGC 
ATTTATCCCT GGTACGCTGT TAGAAATTTT TTGGAAAAGT TTGTGCAAGG CTTAGATATA 
GGCCCATCGA GCACTCAAGT AAGCCTCATC CAGTATGGAA ATGACCCCCA AGTGATGTAT 
AAAATGGACA CATATAGAAC CAAAGAAGAA ATGGCCAAGG CAATAACTGG TATTGATCAA 
ATGGGTGGAA CTCAGACAAA CACTTTCAAA GCAATTGATT TTACAAGAAA GTTTGCTTTC 
TCTAAAGAAT ACGGCGGGAG GCCCAATGCC AACAAAGTTA TGGTTGTAAT CACAGATGGG 
GAATCACATG ACAATGCTCT CCTATCAAAA GTGATCGGCG ACTGTGAGAA GGACAATATC 
ATTAGATTTG GCATAGCGGT TTTAGGTTAC TACAACAGGT ACAATATCGA TAGCAAAAAT 
CTGATCAAAG AAATCGAGTC CATCGCCAGC GTTCCGAAGG AGAAATTCTT TTTTAATGTG 
TCTGATGAGG TTGCCTTGTT AGAGAAAGCG GGGACCTTGG GCGAGCGCAT TTTTAGTATT 
GAAGGCACTA CACAAGGAGG TGAGACTTTT CAAATGGAAA TGTCCCAAGT TGGTTTCAGT 
GCACAATATT CAGAAAATCA GGGAACACTG ATGTTGGGAG CGGTCGGCGC CTATGACTGG 
AGCGGCACAG TAGTGCATGA AAAAGCAAAG CAGTTTTCTG TTTTTCCATA TAAAGCATTT 
GAAAAGGTCC TGCATGATCG GAATCAAAGC TCATATCTGG GATATTCTGT CGCTGTTATA 
AATATGAAAA ACTCAGTGAA CTTCGTTGCT GGTGCTCCAA GAACAGACTA CACAGGTCAA 
GTCGTAGTGT ATAATATTAC CAGCCGGGGA AATGTCTCCG TCATTCAGAT CCAAAGAGGA 
GAACAGATTG GTTCGTATTT TGGGAGTGTT TTATGCTCTG TGGATGTCAA CAGAGATTCC 
ATTACTGACG TCTTATTAGT AGGAGCCCCT ACATTCATGA ATGAGTACAA GAAAGAAGAA 
GGCCAAGTGT ACATGTTCTC TATAAGAGAT GGAATTTTAG TTCAGCGTGA ACAGCTGGAA 
GGTCCTAAAA GCCTGGAGAA CACCAGGTTT GGCTCAGCCA TAGCAGAGCT CTCTGATATT 
GACTTGGATG GGTATAATGA TGTCATAGTC GGAGCGCCTT TGGAAAACCA GAACAGCGGA 
GCAATTTATA TTTTTAATGG AAACCAAAAC ACAATAAGAA CCAAATATTC CCAGAAAATT 
TTGGGTTCTT CATTCAACCC AGGGCTGCAG TACTTTGGGA GATCTGTGGA TGGACACCAG 
GATTTAAATG GTGACACAAT AAATGATGTA TCGGTGGGGG CTTATGGAAA AGCTATTCAG 
TTATGGTCTC AGCATATAGC TGATGTGTCA GTGAAGGCGA TATTTATTCC ACAACAAATC 
ATTCTTACAA ACAAGAACTG TGAGGTGACA GTGAGAATAT GCTTCAGCGC CGTATTCAGA 
CCCACTAACT CCCAGAACTC CGTAGCTGTG ACATATAACG CCACCTTGGA TGCAGATCTT 
CTCTCATCAA GAGTGACATC TAGAGGACAA TTTAGGGAGA GCAACGACCG GTTCTTACAG 
AGGAATATTG CCATTGGACC CACAGAAAGC TGTTTCGAAC ACATATTCAA TGTTCAGGAG 
ACTTCCAATA CGGAGAATGC TCTTGCTCTA CGGATTAATG TCATTGCACA GAACCCAGGG 
ACAAGCCCAG TCTTAAACCC TTATTCATCA AGTTCCACAG AATGGTTTAT TCCATTCCTG 
AAAGACTGCG GTGATGACAA AATCTGTATT TCAGACCTCT CTCTGCAGAT TCAGCAGATA 
CCAAATGATA TCAAACTGCC CTACATTGTG AGTATGAAGG ACAGGCGCTT ATTGTTCCAA 
GTAACAGTGA CCAACAAACA GGAGAATGCC TATAACACAA GGCTCAACGC TGTCTTCTCA 
GAGAACTTGT TCTTTGCATC CTCTACAACT CCAAGGGATG GAACGGAAGT GTTATGCCAA 
GTTGGTAATG CACAAAATTC AGTCAGCTGC TTGATTGGTT TTCCTTTTCT TAAGGACGGA 
CAAAAGGTTA TATTCGACAT TTGGTTCGAT TTCAATCTGA ACAATCTGCA GAATAAAGCC 
TTTATTTATT TCCAGACAAT GAGTGACAGC AGAGAAGCTC GGGAAAACGA CAATTCAGTC 
AACATTTCTT TGCCAGTTCA GTATGATGCG GAAATCCACC TTACAAGGGA GGCCAACATA 
AATTTTTATG AAGTCTTTCC TGGTTTAAAT ATCCCATCGG TGGTAAACAA GTTTGATGAT 
ATTGGGCTAG AATTCAATTT CTCAGTAAAA GTTAGCACTG GAACAGTTCC ACTAAAAACA 
GCTTTTCTCT CCATTAATGT GCCACAGTTC ACAAAATCAA ACAATCCTCT GCTGTACATC 
ACTTCAGTCC AAACAGACCA GATGAGTGGT GTTTCTTGCA ATGCGAATAT AAATCCTGAC 
CACATAGGAA GAATTCCATA TGAAACATCA TTCAAAGAAG AGAACCTTAA AAATGAGTTG 
AACTGTAAGA GCGCCCGCTG TGCCGCCATC CAATGTTCCC TGGAAGGCCT GCAGCTCCGA 
GAAGATTATT ATGTAAATGT CTCTACCAGA ATCTGGAACG GAACGTTTGC AGCTGCACAT 
TTCCAGACTA TAGAGTTAGT GGCAAATGCA AAAATTGAAA CAATGGACTC AGAAATATTC 
GTCATCAGCA ACAAAGACGC AATGATCCCC CTGACAATAA CAAAGCCTGG AGAGAAATCA 
GAAGTTCCAA TTGGCATTGT GATTGGAAGT GCCATTGCTG GGCTTGTTCT TTTAGCTGCG 
CTAGTTGCCC TTTTGTGGAA GCTCGGCTTC TTCAAAAGAA AATATCAAAA ACTTCAGAAA 
ACGGAAGAAG AAATAGCAGA GACAACGCAA CTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itga2 ( XM_002933043.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933089.2 Xenopus tropicalis integrin subunit alpha 2 (itga2), mRNA.

Price & Availability↑

CloneID	OXa03394
Clone ID Related Accession (Same CDS sequence)	XM_002933043.4
Accession Version	XM_002933043.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin alpha-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933043.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAA CAACTTGCAT TCCTTGGCTG CTGCTGGTAA TACTACAAGG AAATCTTTTA 
AAGCACAGTG AAGCATACAA TGTTGGCCTT TCAGGAGCCA AGATATATAG CGGACCTGCC 
AACACGCAGT TTGGGTATTC TGTTCAGCAG TTTCATAACC AAGATGGAAA TTGGTTGCTT 
GTTAGTTCCC CTTGGAGTGG TTATCCAAAA AATAGGATGG GAGACATTTA CAAGTGCTCA 
GTCAGCTCAT CAACGACCAA ATGTGATAAA ATGAATTTAG CGGATGCGAC TATCATTCCC 
AATGTTACTG AGAAAAGAGA AGAAATGAAT CTTGGATTGA CCCTGTTGAG AAATGGAAAT 
ACAGGAGGAT TTTTGGCCTG TGGACCACTG TGGGCACAGC AGTGTGGTAA CAATTACTAC 
GCAACCGGCG TATGCTCTGA TGTTAGCCCA AGTTTTAAAG TAATTAGAAG TTTTTCACCG 
GCTCTTCAAA CATGTTCATC TTATATTGAC ATTGCAATTG TGTGCGATGG ATCAAACAGC 
ATTTATCCCT GGTACGCTGT TAGAAATTTT TTGGAAAAGT TTGTGCAAGG CTTAGATATA 
GGCCCATCGA GCACTCAAGT AAGCCTCATC CAGTATGGAA ATGACCCCCA AGTGATGTAT 
AAAATGGACA CATATAGAAC CAAAGAAGAA ATGGCCAAGG CAATAACTGG TATTGATCAA 
ATGGGTGGAA CTCAGACAAA CACTTTCAAA GCAATTGATT TTACAAGAAA GTTTGCTTTC 
TCTAAAGAAT ACGGCGGGAG GCCCAATGCC AACAAAGTTA TGGTTGTAAT CACAGATGGG 
GAATCACATG ACAATGCTCT CCTATCAAAA GTGATCGGCG ACTGTGAGAA GGACAATATC 
ATTAGATTTG GCATAGCGGT TTTAGGTTAC TACAACAGGT ACAATATCGA TAGCAAAAAT 
CTGATCAAAG AAATCGAGTC CATCGCCAGC GTTCCGAAGG AGAAATTCTT TTTTAATGTG 
TCTGATGAGG TTGCCTTGTT AGAGAAAGCG GGGACCTTGG GCGAGCGCAT TTTTAGTATT 
GAAGGCACTA CACAAGGAGG TGAGACTTTT CAAATGGAAA TGTCCCAAGT TGGTTTCAGT 
GCACAATATT CAGAAAATCA GGGAACACTG ATGTTGGGAG CGGTCGGCGC CTATGACTGG 
AGCGGCACAG TAGTGCATGA AAAAGCAAAG CAGTTTTCTG TTTTTCCATA TAAAGCATTT 
GAAAAGGTCC TGCATGATCG GAATCAAAGC TCATATCTGG GATATTCTGT CGCTGTTATA 
AATATGAAAA ACTCAGTGAA CTTCGTTGCT GGTGCTCCAA GAACAGACTA CACAGGTCAA 
GTCGTAGTGT ATAATATTAC CAGCCGGGGA AATGTCTCCG TCATTCAGAT CCAAAGAGGA 
GAACAGATTG GTTCGTATTT TGGGAGTGTT TTATGCTCTG TGGATGTCAA CAGAGATTCC 
ATTACTGACG TCTTATTAGT AGGAGCCCCT ACATTCATGA ATGAGTACAA GAAAGAAGAA 
GGCCAAGTGT ACATGTTCTC TATAAGAGAT GGAATTTTAG TTCAGCGTGA ACAGCTGGAA 
GGTCCTAAAA GCCTGGAGAA CACCAGGTTT GGCTCAGCCA TAGCAGAGCT CTCTGATATT 
GACTTGGATG GGTATAATGA TGTCATAGTC GGAGCGCCTT TGGAAAACCA GAACAGCGGA 
GCAATTTATA TTTTTAATGG AAACCAAAAC ACAATAAGAA CCAAATATTC CCAGAAAATT 
TTGGGTTCTT CATTCAACCC AGGGCTGCAG TACTTTGGGA GATCTGTGGA TGGACACCAG 
GATTTAAATG GTGACACAAT AAATGATGTA TCGGTGGGGG CTTATGGAAA AGCTATTCAG 
TTATGGTCTC AGCATATAGC TGATGTGTCA GTGAAGGCGA TATTTATTCC ACAACAAATC 
ATTCTTACAA ACAAGAACTG TGAGGTGACA GTGAGAATAT GCTTCAGCGC CGTATTCAGA 
CCCACTAACT CCCAGAACTC CGTAGCTGTG ACATATAACG CCACCTTGGA TGCAGATCTT 
CTCTCATCAA GAGTGACATC TAGAGGACAA TTTAGGGAGA GCAACGACCG GTTCTTACAG 
AGGAATATTG CCATTGGACC CACAGAAAGC TGTTTCGAAC ACATATTCAA TGTTCAGGAG 
ACTTCCAATA CGGAGAATGC TCTTGCTCTA CGGATTAATG TCATTACACA GAACCCAGGG 
ACAAGGTTAT TTAGCAAGAG CAGCTCATCA GTTGACTGTT CTTTTGGTTT GTATCAGATT 
CCATTCCTGA AAGACTGCGG TGATGACAAA ATCTGTATTT CAGACCTCTC TCTGCAGATT 
CAGCAGATAC CAAATGATAT CAAACTGCCC TACATTGTGA GTATGAAGGA CAGGCGCTTA 
TTGTTCCAAG TAACAGTGAC AAACAAACAG GAGAATGCCT ATAACACAAG GCTCAACGCT 
GTCTTCTCAG AGAACTTGTT CTTTGCATCC TCTACAACTC CAAGGGATGG AACGGAAGTG 
TTATGCCAAG TTGGTAATGC ACAAAATTCA GTCAGCTGCT TGATTGGTTT TCCTTTTCTT 
AAGGACGGAC AAAAGGTTAT ATTCGACATT TGGTTCGATT TCAATCTGAA CAATCTGCAG 
AATAAAGCCT TTATTTATTT CCAGACAATG AGTGACAGCA GAGAAGCTCG GGAAAACGAC 
AATTCAGTCA ACATTTCTTT GCCAGTTCAG TATGATGCGG AAATCCACCT TACAAGGGAG 
GCCAACATAA ATTTTTATGA AGTCTTTCCT GGTTTAAATA TCCCATCGGT GGTAAACAAG 
TTTGATGATA TTGGGCTAGA ATTCAATTTC TCAGTAAAAG TTAGCACTGG AACAGTTCCA 
CTAAAAACAG CTTTTCTCTC CATTAATGTG CCACAGTTCA CAAAATCAAA CAATCCTCTG 
CTGTACATCA CTTCAGTCCA AACAGACCAG ATGAGTGGTG TTTCTTGCAA TGCGAATATA 
AATCCTGACC ACATAGGAAG AATTCCATAT GAACCATCAT TCAAAGAAGA GAACCTTAAA 
AATGAGTTGA ACTGTAAGAG CGCCCGCTGT GCCGCCATCC AATGTTCCCT GGAAGGCCTG 
CAGCTCCGAG AAGATTATTA TGTAAATGTC TCTACCAGAA TCTGGAACGG AACGTTTGCA 
GCTGCACATT TCCAGACTAT AGAGTTAGTG GCAAATGCAA AAATTGAAAC AATGGACTCA 
GAAATATTCG TCATCAGCAA CAAAGACGCA ATGATCCCCC TGACAATAAC AAAGCCTGGA 
GAGAAATCAG AAGTTCCAAT TGGCATTGTG ATTGGAAGTG CCATTGCTGG GCTTGTTCTT 
TTAGCTGCGC TAGTTGCCCT TTTGTGGAAG CTCGGCTTCT TCAAAAGAAA ATATCAAAAA 
CTTCAGAAAA CGGAAGAAGA AATAGCAGAG ACAACGCAAC TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933089.2
CDS	217..3744
Translation	MARTTCIPWLLLVILQGNLLKHSEAYNVGLSGAKIYSGPANTQFGYSVQQFHNQDGNWLLVSSPWSGYPKNRMGDIYKCSVSSSTTKCDKMNLADATIIPNVTEKREEMNLGLTLLRNGNTGGFLACGPLWAQQCGNNYYATGVCSDVSPSFKVIRSFSPALQTCSSYIDIAIVCDGSNSIYPWYAVRNFLEKFVQGLDIGPSSTQVSLIQYGNDPQVMYKMDTYRTKEEMAKAITGIDQMGGTQTNTFKAIDFTRKFAFSKEYGGRPNANKVMVVITDGESHDNALLSKVIGDCEKDNIIRFGIAVLGYYNRYNIDSKNLIKEIESIASVPKEKFFFNVSDEVALLEKAGTLGERIFSIEGTTQGGETFQMEMSQVGFSAQYSENQGTLMLGAVGAYDWSGTVVHEKAKQFSVFPYKAFEKVLHDRNQSSYLGYSVAVINMKNSVNFVAGAPRTDYTGQVVVYNITSRGNVSVIQIQRGEQIGSYFGSVLCSVDVNRDSITDVLLVGAPTFMNEYKKEEGQVYMFSIRDGILVQREQLEGPKSLENTRFGSAIAELSDIDLDGYNDVIVGAPLENQNSGAIYIFNGNQNTIRTKYSQKILGSSFNPGLQYFGRSVDGHQDLNGDTINDVSVGAYGKAIQLWSQHIADVSVKAIFIPQQIILTNKNCEVTVRICFSAVFRPTNSQNSVAVTYNATLDADLLSSRVTSRGQFRESNDRFLQRNIAIGPTESCFEHIFNVQETSNTENALALRINVITQNPGTRLFSKSSSSVDCSFGLYQIPFLKDCGDDKICISDLSLQIQQIPNDIKLPYIVSMKDRRLLFQVTVTNKQENAYNTRLNAVFSENLFFASSTTPRDGTEVLCQVGNAQNSVSCLIGFPFLKDGQKVIFDIWFDFNLNNLQNKAFIYFQTMSDSREARENDNSVNISLPVQYDAEIHLTREANINFYEVFPGLNIPSVVNKFDDIGLEFNFSVKVSTGTVPLKTAFLSINVPQFTKSNNPLLYITSVQTDQMSGVSCNANINPDHIGRIPYEPSFKEENLKNELNCKSARCAAIQCSLEGLQLREDYYVNVSTRIWNGTFAAAHFQTIELVANAKIETMDSEIFVISNKDAMIPLTITKPGEKSEVPIGIVIGSAIAGLVLLAALVALLWKLGFFKRKYQKLQKTEEEIAETTQLN

Target ORF information:

RefSeq Version	XM_002933043.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit alpha 2 (itga2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933043.4

ORF Insert Sequence:

ATGGCGAGAA CAACTTGCAT TCCTTGGCTG CTGCTGGTAA TACTACAAGG AAATCTTTTA 
AAGCACAGTG AAGCATACAA TGTTGGCCTT TCAGGAGCCA AGATATATAG CGGACCTGCC 
AACACGCAGT TTGGGTATTC TGTTCAGCAG TTTCATAACC AAGATGGAAA TTGGTTGCTT 
GTTAGTTCCC CTTGGAGTGG TTATCCAAAA AATAGGATGG GAGACATTTA CAAGTGCTCA 
GTCAGCTCAT CAACGACCAA ATGTGATAAA ATGAATTTAG CGGATGCGAC TATCATTCCC 
AATGTTACTG AGAAAAGAGA AGAAATGAAT CTTGGATTGA CCCTGTTGAG AAATGGAAAT 
ACAGGAGGAT TTTTGGCCTG TGGACCACTG TGGGCACAGC AGTGTGGTAA CAATTACTAC 
GCAACCGGCG TATGCTCTGA TGTTAGCCCA AGTTTTAAAG TAATTAGAAG TTTTTCACCG 
GCTCTTCAAA CATGTTCATC TTATATTGAC ATTGCAATTG TGTGCGATGG ATCAAACAGC 
ATTTATCCCT GGTACGCTGT TAGAAATTTT TTGGAAAAGT TTGTGCAAGG CTTAGATATA 
GGCCCATCGA GCACTCAAGT AAGCCTCATC CAGTATGGAA ATGACCCCCA AGTGATGTAT 
AAAATGGACA CATATAGAAC CAAAGAAGAA ATGGCCAAGG CAATAACTGG TATTGATCAA 
ATGGGTGGAA CTCAGACAAA CACTTTCAAA GCAATTGATT TTACAAGAAA GTTTGCTTTC 
TCTAAAGAAT ACGGCGGGAG GCCCAATGCC AACAAAGTTA TGGTTGTAAT CACAGATGGG 
GAATCACATG ACAATGCTCT CCTATCAAAA GTGATCGGCG ACTGTGAGAA GGACAATATC 
ATTAGATTTG GCATAGCGGT TTTAGGTTAC TACAACAGGT ACAATATCGA TAGCAAAAAT 
CTGATCAAAG AAATCGAGTC CATCGCCAGC GTTCCGAAGG AGAAATTCTT TTTTAATGTG 
TCTGATGAGG TTGCCTTGTT AGAGAAAGCG GGGACCTTGG GCGAGCGCAT TTTTAGTATT 
GAAGGCACTA CACAAGGAGG TGAGACTTTT CAAATGGAAA TGTCCCAAGT TGGTTTCAGT 
GCACAATATT CAGAAAATCA GGGAACACTG ATGTTGGGAG CGGTCGGCGC CTATGACTGG 
AGCGGCACAG TAGTGCATGA AAAAGCAAAG CAGTTTTCTG TTTTTCCATA TAAAGCATTT 
GAAAAGGTCC TGCATGATCG GAATCAAAGC TCATATCTGG GATATTCTGT CGCTGTTATA 
AATATGAAAA ACTCAGTGAA CTTCGTTGCT GGTGCTCCAA GAACAGACTA CACAGGTCAA 
GTCGTAGTGT ATAATATTAC CAGCCGGGGA AATGTCTCCG TCATTCAGAT CCAAAGAGGA 
GAACAGATTG GTTCGTATTT TGGGAGTGTT TTATGCTCTG TGGATGTCAA CAGAGATTCC 
ATTACTGACG TCTTATTAGT AGGAGCCCCT ACATTCATGA ATGAGTACAA GAAAGAAGAA 
GGCCAAGTGT ACATGTTCTC TATAAGAGAT GGAATTTTAG TTCAGCGTGA ACAGCTGGAA 
GGTCCTAAAA GCCTGGAGAA CACCAGGTTT GGCTCAGCCA TAGCAGAGCT CTCTGATATT 
GACTTGGATG GGTATAATGA TGTCATAGTC GGAGCGCCTT TGGAAAACCA GAACAGCGGA 
GCAATTTATA TTTTTAATGG AAACCAAAAC ACAATAAGAA CCAAATATTC CCAGAAAATT 
TTGGGTTCTT CATTCAACCC AGGGCTGCAG TACTTTGGGA GATCTGTGGA TGGACACCAG 
GATTTAAATG GTGACACAAT AAATGATGTA TCGGTGGGGG CTTATGGAAA AGCTATTCAG 
TTATGGTCTC AGCATATAGC TGATGTGTCA GTGAAGGCGA TATTTATTCC ACAACAAATC 
ATTCTTACAA ACAAGAACTG TGAGGTGACA GTGAGAATAT GCTTCAGCGC CGTATTCAGA 
CCCACTAACT CCCAGAACTC CGTAGCTGTG ACATATAACG CCACCTTGGA TGCAGATCTT 
CTCTCATCAA GAGTGACATC TAGAGGACAA TTTAGGGAGA GCAACGACCG GTTCTTACAG 
AGGAATATTG CCATTGGACC CACAGAAAGC TGTTTCGAAC ACATATTCAA TGTTCAGGAG 
ACTTCCAATA CGGAGAATGC TCTTGCTCTA CGGATTAATG TCATTACACA GAACCCAGGG 
ACAAGGTTAT TTAGCAAGAG CAGCTCATCA GTTGACTGTT CTTTTGGTTT GTATCAGATT 
CCATTCCTGA AAGACTGCGG TGATGACAAA ATCTGTATTT CAGACCTCTC TCTGCAGATT 
CAGCAGATAC CAAATGATAT CAAACTGCCC TACATTGTGA GTATGAAGGA CAGGCGCTTA 
TTGTTCCAAG TAACAGTGAC AAACAAACAG GAGAATGCCT ATAACACAAG GCTCAACGCT 
GTCTTCTCAG AGAACTTGTT CTTTGCATCC TCTACAACTC CAAGGGATGG AACGGAAGTG 
TTATGCCAAG TTGGTAATGC ACAAAATTCA GTCAGCTGCT TGATTGGTTT TCCTTTTCTT 
AAGGACGGAC AAAAGGTTAT ATTCGACATT TGGTTCGATT TCAATCTGAA CAATCTGCAG 
AATAAAGCCT TTATTTATTT CCAGACAATG AGTGACAGCA GAGAAGCTCG GGAAAACGAC 
AATTCAGTCA ACATTTCTTT GCCAGTTCAG TATGATGCGG AAATCCACCT TACAAGGGAG 
GCCAACATAA ATTTTTATGA AGTCTTTCCT GGTTTAAATA TCCCATCGGT GGTAAACAAG 
TTTGATGATA TTGGGCTAGA ATTCAATTTC TCAGTAAAAG TTAGCACTGG AACAGTTCCA 
CTAAAAACAG CTTTTCTCTC CATTAATGTG CCACAGTTCA CAAAATCAAA CAATCCTCTG 
CTGTACATCA CTTCAGTCCA AACAGACCAG ATGAGTGGTG TTTCTTGCAA TGCGAATATA 
AATCCTGACC ACATAGGAAG AATTCCATAT GAACCATCAT TCAAAGAAGA GAACCTTAAA 
AATGAGTTGA ACTGTAAGAG CGCCCGCTGT GCCGCCATCC AATGTTCCCT GGAAGGCCTG 
CAGCTCCGAG AAGATTATTA TGTAAATGTC TCTACCAGAA TCTGGAACGG AACGTTTGCA 
GCTGCACATT TCCAGACTAT AGAGTTAGTG GCAAATGCAA AAATTGAAAC AATGGACTCA 
GAAATATTCG TCATCAGCAA CAAAGACGCA ATGATCCCCC TGACAATAAC AAAGCCTGGA 
GAGAAATCAG AAGTTCCAAT TGGCATTGTG ATTGGAAGTG CCATTGCTGG GCTTGTTCTT 
TTAGCTGCGC TAGTTGCCCT TTTGTGGAAG CTCGGCTTCT TCAAAAGAAA ATATCAAAAA 
CTTCAGAAAA CGGAAGAAGA AATAGCAGAG ACAACGCAAC TGAACTGA