rapgef3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rapgef3
Entrez Gene ID	100491495
Full Name	Rap guanine nucleotide exchange factor 3
Synonyms	Epac,XEpac
General protein information	Preferred Names Rap guanine nucleotide exchange factor 3 Names rap guanine nucleotide exchange factor 3 Rap guanine nucleotide exchange factor (GEF) 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012957843.2	XP_012813297.1	rap guanine nucleotide exchange factor 3
XM_012957843.3	XP_012813297.1	rap guanine nucleotide exchange factor 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF3	XP_001162479.2
Xenopus tropicalis (tropical clawed frog)	rapgef3	XP_002935702.2
Rattus norvegicus (Norway rat)	Rapgef3	NP_067722.1
Danio rerio (zebrafish)	LOC101886094	XP_005162410.1
	RAPGEF3	XP_001098291.2
Gallus gallus (chicken)	RAPGEF3	XP_424494.4
Bos taurus (cattle)	RAPGEF3	NP_001073771.1
Homo sapiens (human)	RAPGEF3	NP_001092001.1
Canis lupus familiaris (dog)	RAPGEF3	NP_001093414.1
Mus musculus (house mouse)	Rapgef3	NP_001171281.1

Gene Ontology

The following rapgef3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa35066	XM_012957843.2 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 3 (rapgef3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa52720	XM_012957843.3 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 3 (rapgef3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rapgef3 ( XM_012957843.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813297.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 3 (rapgef3), mRNA.

Price & Availability↑

CloneID	OXa35066
Clone ID Related Accession (Same CDS sequence)	XM_012957843.2
Accession Version	XM_012957843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAA TAGGGCAGCA GCTGCGGAAT CCCTGTGGTA TGAGGCACAG GAACAAGATG 
CACTTCTTCA GGACCTACTC CTATGAGATT CAGGTGGAGC AGAGATTGGA TATTCACAGC 
CGCATCCAAG GCATCAGATG GACACCGTTA CCTGATATTA AGTCTCAAGA AGAATACAAG 
CAGACTGTGA AACAGGTGGT AACACATAAA GTCTGGAGGG CAGCAAAAGT TTTGCACACT 
TACCTGCTTA CCGTGTCTCC AGCCATGATG CGTGACAGGA AACACCATTT CCGCAGCCAC 
CGACAATGCT GCTCAGGGAA GGAGCTTGTT GACTGGTTAC TGGGCATCAG TCATGGCCTG 
CAGTCCCGAA GCCAAGCCGT GGGGATATGG CAGGTGCTGG TGGATGAAGG AGCCCTTGTG 
CATGTGAGAC AAGAGCCCAA TTTCCATGAT AAAGACTCCC AATTTTATCG ATTTGTGAAA 
GCAGAACTAC ACGTTGACCC CTTGCTGACT GACAAAGATA CAGAAGAAGA AGTGCAAGAG 
TCCTTGGTAT TACTGTCTCA GTTGGGACCA GCAGCGTTGC TAACCATGAT TTTACGCAAA 
CCCCCTTGCC AGCGTACTGA AGAAGAGCTG GAAGTGATAT TTGAGGAGTT ACTTCACATC 
AAGGCGGTGT CCCATCTTTC CAACTCGGTG AAAAAGGAAT TGGCTTCTGT TTTGATATTT 
GAGTCTCATG CGAAAGCTGG TACAGTGTTG TTCAATCAAG GAGATAAGGG CACCTCCTGG 
TATATTATAT GGAAAGGTTC TGTAAACGTA ATTACACATG GGAAGGGCCT GGTTGCTACA 
CTTCACGAGG GAGATGATTT TGGGCAGTTG GCTCTTGTAA ATGATGCCCC AAGAGCTGCT 
ACCATAGTCT TGCGGGAAGA CAACTGCCAT TTTCTGCGTG TGGACAAGCA TGACTTCAAT 
CGCATTCTTC GGGATGTAGA AGCTAACACA GTTCGTCTGA AAGAACATGG GAAGGTGGTA 
TTAGTTTTGG AGAAAAATCC ACAAATATCA GCTGGAAACA ACCACCAACC AGCAGGGGCC 
TCCAACCAAA AGTACACTGT CATGTCTGGG ACAGCTGATA AAATCCTGGA ACACTTACTA 
GAGACTATAA AGCTGGATTC ATGCACAGAA GACTCCTTTG CAGGTGATTT CCTCTTGACT 
CACAGTATCT TCATGCCAAC AACCCAACTG TGCCAAGCTT TGCTGCACCA GTTCAACGTG 
GAGCCGTCAG AAGTGAATGA GCCAGAAAAG GCAACCTATT CCCTTCATAA GCGGCAGAAC 
ATCCTGCACC TGGTGAGCCT GTGGGTAGCT CTGTATGGGA AGTTACTGGA GGCTGAGCCA 
AGTGCCAAGA GCTTCCTAGA GAAACTTTCA GAACATGTCA GCAGAGATCC ACGGCTCCTT 
GCCTTTTGGA GAGAGAATCT TCAGGATAGG CGGAAAACCA GAACATTGGA GGTGAGCAGT 
GGGAATCACT CTCCCCAAAT AAAGATCAAC AGCTTTGGGA ACCTTTTTTC CAGTCTGGAG 
GAGGACGCAT CTAGTAACAC ATATACTATT CGAGCAGGAG ACAAAGTGCC TTATGATATT 
TATCGACCAG ACCACTCCTG CATCACCACA ATGCTGCCTG TGAATGCCTC GGTGCAAGAG 
GTGATATCAT CATTAGCAAC AAAAAATGGA TATAACAGAG ATCAAATCGT GGTAAAAGTC 
AACTCAAGTG GAGAAAGGGT CTGTCTAAAG CATGAGGACA CTGGCGTATT CACGTCTTTA 
GGGCTCAACG AAAGAATTTT CATCTGCTAC AATCATGAAC TGAACAATGT GACCCCCCTC 
CCTGAACAAC TGGGACCAAA CACCAGCAGC TCTGATATTC TGGAACTGAT CAGTTCAAAG 
GACCTGGCTC ACCAGATGAC GGATAATGAC TGGAACCTGT TTAAGAGCAT CCACCAAGTG 
GAGTTGATTT ATCACACGTT TGGGAAGCAG AGGTTTCGTA ACGCAACTAC TGCTAACCTT 
GAGCGGTTCA TGAGGCGATT CAATGAAGTT CAGTTCTGGG TGGCTACTGA GGTATGCCTA 
TGCCAAGAGG AGGAGCGGAG AGCACTGCTT CTAAAAAAGT TCATCAAACT CGCCGCTTAC 
CTCAAGGAGC AGAAAAATCT GAACTCATTC TTTGCAGTAA TGTTCGGTCT CAGTAACACA 
GCCGTCAGCC GCTTGTCCAG AACCTGGCAG AAACTGCCAA ACAAATTCAG GAAGCTATAT 
TCAATATTTG AGAGGCTCAT GGACCCCTCG TGGAACCATC GATCTTACAG ACTTGCTGTA 
GCAAAACTAA GTGCCCCTCT TATCCCATTT CTTCCACTTA TCCTTAAAGA CCTCACGTTC 
CTACATGAGG GAAACAGAAG CACATTGGAC AGCCTGGTAA ACTTTGAGAA AATGCGCATG 
ATTGCAAAGA CAGTTCAGAT GTTCCACAGC TGCCGCAGCC AGGCCTACAT TCCTCTCTCA 
CCTTTGAGAC CTCGACCTGT AAACATCCTT GAGGATCCTA GTGCTGCGCG GATATCTGCA 
TGTTCAGAGC ACTCAATGAC GTTAAGAAGC CCAGCTACAA CATGGGCTTA TATTCAGCAT 
ATGAAAGTGA TTGATAATCA GAAGAAACTG ATGGAATTAT CCCGTAGCCT GGAGCCTGCC 
TCTTCTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813297.1
CDS	790..3501
Translation	MRIIGQQLRNPCGMRHRNKMHFFRTYSYEIQVEQRLDIHSRIQGIRWTPLPDIKSQEEYKQTVKQVVTHKVWRAAKVLHTYLLTVSPAMMRDRKHHFRSHRQCCSGKELVDWLLGISHGLQSRSQAVGIWQVLVDEGALVHVRQEPNFHDKDSQFYRFVKAELHVDPLLTDKDTEEEVQESLVLLSQLGPAALLTMILRKPPCQRTEEELEVIFEELLHIKAVSHLSNSVKKELASVLIFESHAKAGTVLFNQGDKGTSWYIIWKGSVNVITHGKGLVATLHEGDDFGQLALVNDAPRAATIVLREDNCHFLRVDKHDFNRILRDVEANTVRLKEHGKVVLVLEKNPQISAGNNHQPAGASNQKYTVMSGTADKILEHLLETIKLDSCTEDSFAGDFLLTHSIFMPTTQLCQALLHQFNVEPSEVNEPEKATYSLHKRQNILHLVSLWVALYGKLLEAEPSAKSFLEKLSEHVSRDPRLLAFWRENLQDRRKTRTLEVSSGNHSPQIKINSFGNLFSSLEEDASSNTYTIRAGDKVPYDIYRPDHSCITTMLPVNASVQEVISSLATKNGYNRDQIVVKVNSSGERVCLKHEDTGVFTSLGLNERIFICYNHELNNVTPLPEQLGPNTSSSDILELISSKDLAHQMTDNDWNLFKSIHQVELIYHTFGKQRFRNATTANLERFMRRFNEVQFWVATEVCLCQEEERRALLLKKFIKLAAYLKEQKNLNSFFAVMFGLSNTAVSRLSRTWQKLPNKFRKLYSIFERLMDPSWNHRSYRLAVAKLSAPLIPFLPLILKDLTFLHEGNRSTLDSLVNFEKMRMIAKTVQMFHSCRSQAYIPLSPLRPRPVNILEDPSAARISACSEHSMTLRSPATTWAYIQHMKVIDNQKKLMELSRSLEPASST

Target ORF information:

RefSeq Version	XM_012957843.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 3 (rapgef3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957843.2

ORF Insert Sequence:

ATGAGAATAA TAGGGCAGCA GCTGCGGAAT CCCTGTGGTA TGAGGCACAG GAACAAGATG 
CACTTCTTCA GGACCTACTC CTATGAGATT CAGGTGGAGC AGAGATTGGA TATTCACAGC 
CGCATCCAAG GCATCAGATG GACACCGTTA CCTGATATTA AGTCTCAAGA AGAATACAAG 
CAGACTGTGA AACAGGTGGT AACACATAAA GTCTGGAGGG CAGCAAAAGT TTTGCACACT 
TACCTGCTTA CCGTGTCTCC AGCCATGATG CGTGACAGGA AACACCATTT CCGCAGCCAC 
CGACAATGCT GCTCAGGGAA GGAGCTTGTT GACTGGTTAC TGGGCATCAG TCATGGCCTG 
CAGTCCCGAA GCCAAGCCGT GGGGATATGG CAGGTGCTGG TGGATGAAGG AGCCCTTGTG 
CATGTGAGAC AAGAGCCCAA TTTCCATGAT AAAGACTCCC AATTTTATCG ATTTGTGAAA 
GCAGAACTAC ACGTTGACCC CTTGCTGACT GACAAAGATA CAGAAGAAGA AGTGCAAGAG 
TCCTTGGTAT TACTGTCTCA GTTGGGACCA GCAGCGTTGC TAACCATGAT TTTACGCAAA 
CCCCCTTGCC AGCGTACTGA AGAAGAGCTG GAAGTGATAT TTGAGGAGTT ACTTCACATC 
AAGGCGGTGT CCCATCTTTC CAACTCGGTG AAAAAGGAAT TGGCTTCTGT TTTGATATTT 
GAGTCTCATG CGAAAGCTGG TACAGTGTTG TTCAATCAAG GAGATAAGGG CACCTCCTGG 
TATATTATAT GGAAAGGTTC TGTAAACGTA ATTACACATG GGAAGGGCCT GGTTGCTACA 
CTTCACGAGG GAGATGATTT TGGGCAGTTG GCTCTTGTAA ATGATGCCCC AAGAGCTGCT 
ACCATAGTCT TGCGGGAAGA CAACTGCCAT TTTCTGCGTG TGGACAAGCA TGACTTCAAT 
CGCATTCTTC GGGATGTAGA AGCTAACACA GTTCGTCTGA AAGAACATGG GAAGGTGGTA 
TTAGTTTTGG AGAAAAATCC ACAAATATCA GCTGGAAACA ACCACCAACC AGCAGGGGCC 
TCCAACCAAA AGTACACTGT CATGTCTGGG ACAGCTGATA AAATCCTGGA ACACTTACTA 
GAGACTATAA AGCTGGATTC ATGCACAGAA GACTCCTTTG CAGGTGATTT CCTCTTGACT 
CACAGTATCT TCATGCCAAC AACCCAACTG TGCCAAGCTT TGCTGCACCA GTTCAACGTG 
GAGCCGTCAG AAGTGAATGA GCCAGAAAAG GCAACCTATT CCCTTCATAA GCGGCAGAAC 
ATCCTGCACC TGGTGAGCCT GTGGGTAGCT CTGTATGGGA AGTTACTGGA GGCTGAGCCA 
AGTGCCAAGA GCTTCCTAGA GAAACTTTCA GAACATGTCA GCAGAGATCC ACGGCTCCTT 
GCCTTTTGGA GAGAGAATCT TCAGGATAGG CGGAAAACCA GAACATTGGA GGTGAGCAGT 
GGGAATCACT CTCCCCAAAT AAAGATCAAC AGCTTTGGGA ACCTTTTTTC CAGTCTGGAG 
GAGGACGCAT CTAGTAACAC ATATACTATT CGAGCAGGAG ACAAAGTGCC TTATGATATT 
TATCGACCAG ACCACTCCTG CATCACCACA ATGCTGCCTG TGAATGCCTC GGTGCAAGAG 
GTGATATCAT CATTAGCAAC AAAAAATGGA TATAACAGAG ATCAAATCGT GGTAAAAGTC 
AACTCAAGTG GAGAAAGGGT CTGTCTAAAG CATGAGGACA CTGGCGTATT CACGTCTTTA 
GGGCTCAACG AAAGAATTTT CATCTGCTAC AATCATGAAC TGAACAATGT GACCCCCCTC 
CCTGAACAAC TGGGACCAAA CACCAGCAGC TCTGATATTC TGGAACTGAT CAGTTCAAAG 
GACCTGGCTC ACCAGATGAC GGATAATGAC TGGAACCTGT TTAAGAGCAT CCACCAAGTG 
GAGTTGATTT ATCACACGTT TGGGAAGCAG AGGTTTCGTA ACGCAACTAC TGCTAACCTT 
GAGCGGTTCA TGAGGCGATT CAATGAAGTT CAGTTCTGGG TGGCTACTGA GGTATGCCTA 
TGCCAAGAGG AGGAGCGGAG AGCACTGCTT CTAAAAAAGT TCATCAAACT CGCCGCTTAC 
CTCAAGGAGC AGAAAAATCT GAACTCATTC TTTGCAGTAA TGTTCGGTCT CAGTAACACA 
GCCGTCAGCC GCTTGTCCAG AACCTGGCAG AAACTGCCAA ACAAATTCAG GAAGCTATAT 
TCAATATTTG AGAGGCTCAT GGACCCCTCG TGGAACCATC GATCTTACAG ACTTGCTGTA 
GCAAAACTAA GTGCCCCTCT TATCCCATTT CTTCCACTTA TCCTTAAAGA CCTCACGTTC 
CTACATGAGG GAAACAGAAG CACATTGGAC AGCCTGGTAA ACTTTGAGAA AATGCGCATG 
ATTGCAAAGA CAGTTCAGAT GTTCCACAGC TGCCGCAGCC AGGCCTACAT TCCTCTCTCA 
CCTTTGAGAC CTCGACCTGT AAACATCCTT GAGGATCCTA GTGCTGCGCG GATATCTGCA 
TGTTCAGAGC ACTCAATGAC GTTAAGAAGC CCAGCTACAA CATGGGCTTA TATTCAGCAT 
ATGAAAGTGA TTGATAATCA GAAGAAACTG ATGGAATTAT CCCGTAGCCT GGAGCCTGCC 
TCTTCTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef3 ( XM_012957843.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813297.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 3 (rapgef3), mRNA.

Price & Availability↑

CloneID	OXa52720
Clone ID Related Accession (Same CDS sequence)	XM_012957843.3
Accession Version	XM_012957843.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAA TAGGGCAGCA GCTGCGGAAT CCCTGTGGTA TGAGGCACAG GAACAAGATG 
CACTTCTTCA GGACCTACTC CTATGAGATT CAGGTGGAGC AGAGATTGGA TATTCACAGC 
CGCATCCAAG GCATCAGATG GACACCGTTA CCTGATATTA AGTCTCAAGA AGAATACAAG 
CAGACTGTGA AACAGGTGGT AACACATAAA GTCTGGAGGG CAGCAAAAGT TTTGCACACT 
TACCTGCTTA CCGTGTCTCC AGCCATGATG CGTGACAGGA AACACCATTT CCGCAGCCAC 
CGACAATGCT GCTCAGGGAA GGAGCTTGTT GACTGGTTAC TGGGCATCAG TCATGGCCTG 
CAGTCCCGAA GCCAAGCCGT GGGGATATGG CAGGTGCTGG TGGATGAAGG AGCCCTTGTG 
CATGTGAGAC AAGAGCCCAA TTTCCATGAT AAAGACTCCC AATTTTATCG ATTCGTGAAA 
GCAGAACTAC ACGTTGACCC CTTGCTGACT GACAAAGATA CAGAAGAAGA AGTGCAAGAG 
TCCTTGGTAT TACTGTCTCA GTTGGGACCA GCAGCGTTGC TAACCATGAT TTTACGCAAA 
CCCCCTTGCC AGCGTACTGA AGAAGAGCTG GAAGTGATAT TTGAGGAGTT ACTTCACATC 
AAGGCGGTGT CCCATCTTTC CAACTCGGTG AAAAAGGAAT TGGCTTCTGT TTTGATATTT 
GAGTCTCATG CGAAAGCTGG TACAGTGTTG TTCAATCAAG GAGATAAGGG CACCTCCTGG 
TATATTATAT GGAAAGGTTC TGTAAACGTA ATTACACATG GGAAGGGCCT GGTTGCTACA 
CTTCACGAGG GAGATGATTT TGGGCAGTTG GCTCTTGTAA ATGATGCCCC AAGAGCTGCT 
ACCATAGTCT TGCGGGAAGA CAACTGCCAT TTTCTGCGTG TGGACAAGCA TGACTTCAAT 
CGCATTCTTC GGGATGTAGA AGCTAACACA GTTCGTCTGA AAGAACATGG GAAGGTGGTA 
TTAGTTTTGG AGAAAAATCC ACAAATATCA GCTGGAAACA ACCACCAACC AGCAGGGGCC 
TCCAACCAAA AGTACACTGT CATGTCTGGG ACAGCTGATA AAATCCTGGA ACACTTACTA 
GAGACTATAA AGCTGGATTC ATGCACAGAA GACTCCTTTG CAGGTGATTT CCTCTTGACT 
CACAGTATCT TCATGCCAAC AACCCAACTG TGCCAAGCTT TGCTGCACCA GTTCAACGTG 
GAGCCGTCAG AAGTGAATGA GCCAGAAAAG GCAACCTATT CCCTTCATAA GCGGCAGAAC 
ATCCTGCACC TGGTGAGCCT GTGGGTAGCT CTGTATGGGA AGTTACTGGA GGCTGAGCCA 
AGTGCCAAGA GCTTCCTAGA GAAACTTTCA GAACATGTCA GCAGAGATCC ACGGCTCCTT 
GCCTTTTGGA GAGAGAATCT TCAGGATAGG CGGAAAACCA GAACATTGGA GGTGAGCAGT 
GGGAATCACT CTCCCCAAAT AAAGATCAAC AGCTTTGGGA ACCTTTTTTC CAGTCTGGAG 
GAGGACGCAT CTAGTAACAC ATATACTATT CGAGCAGGAG ACAAAGTGCC TTATGATATT 
TATCGACCAG ACCACTCCTG CATCACCACA ATGCTGCCTG TGAATGCCTC GGTGCAAGAG 
GTGATATCAT CATTAGCAAC AAAAAATGGA TATAACAGAG ATCAAATCGT GGTAAAAGTC 
AACTCAAGTG GAGAAAGGGT CTGTCTAAAG CATGAGGACA CTGGCGTATT CACGTCTTTA 
GGGCTCAACG AAAGAATTTT CATCTGCTAC AATCATGAAC TGAACAATGT GACCCCCCTC 
CCTGAACAAC TGGGACCAAA CACCAGCAGC TCTGATATTC TGGAACTGAT CAGTTCAAAG 
GACCTGGCTC ACCAGATGAC GGATAATGAC TGGAACCTGT TTAAGAGCAT CCACCAAGTG 
GAGTTGATTT ATCACACGTT TGGGAAGCAG AGGTTTCGTA ACGCAACTAC TGCTAACCTT 
GAGCGGTTCA TGAGGCGATT CAATGAAGTT CAGTTCTGGG TGGCTACTGA GGTATGCCTA 
TGCCAAGAGG AGGAGCGGAG AGCACTGCTT CTAAAAAAGT TCATCAAACT CGCCGCTTAC 
CTCAAGGAGC AGAAAAATCT GAACTCATTC TTTGCAGTAA TGTTCGGTCT CAGTAACACA 
GCCGTCAGCC GCTTGTCCAG AACCTGGCAG AAACTGCCAA ACAAATTCAG GAAGCTATAT 
TCAATATTTG AGAGGCTCAT GGACCCCTCG TGGAACCATC GATCTTACAG ACTTGCTGTA 
GCAAAACTAA GTGCCCCTCT TATCCCATTT CTTCCACTTA TCCTTAAAGA CCTCACGTTC 
CTACATGAGG GAAACAGAAG CACATTGGAC AGCCTGGTAA ACTTTGAGAA AATGCGCATG 
ATTGCAAAGA CAGTTCAGAT GTTCCACAGC TGCCGCAGCC AGGCCTACAT TCCTCTCTCA 
CCTTTGAGAC CTCGACCTGT AAACATCCTT GAGGATCCTA GTGCTGCGCG GATATCTGCA 
TGTTCAGAGC ACTCAATGAC GTTAAGAAGC CCAGCTACAA CATGGGCTTA TATTCAGCAT 
ATGAAAGTGA TTGATAATCA GAAGAAACTG ATGGAATTGT CCCGTAGCCT GGAGCCTGCC 
TCTTCTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813297.1
CDS	758..3469
Translation	MRIIGQQLRNPCGMRHRNKMHFFRTYSYEIQVEQRLDIHSRIQGIRWTPLPDIKSQEEYKQTVKQVVTHKVWRAAKVLHTYLLTVSPAMMRDRKHHFRSHRQCCSGKELVDWLLGISHGLQSRSQAVGIWQVLVDEGALVHVRQEPNFHDKDSQFYRFVKAELHVDPLLTDKDTEEEVQESLVLLSQLGPAALLTMILRKPPCQRTEEELEVIFEELLHIKAVSHLSNSVKKELASVLIFESHAKAGTVLFNQGDKGTSWYIIWKGSVNVITHGKGLVATLHEGDDFGQLALVNDAPRAATIVLREDNCHFLRVDKHDFNRILRDVEANTVRLKEHGKVVLVLEKNPQISAGNNHQPAGASNQKYTVMSGTADKILEHLLETIKLDSCTEDSFAGDFLLTHSIFMPTTQLCQALLHQFNVEPSEVNEPEKATYSLHKRQNILHLVSLWVALYGKLLEAEPSAKSFLEKLSEHVSRDPRLLAFWRENLQDRRKTRTLEVSSGNHSPQIKINSFGNLFSSLEEDASSNTYTIRAGDKVPYDIYRPDHSCITTMLPVNASVQEVISSLATKNGYNRDQIVVKVNSSGERVCLKHEDTGVFTSLGLNERIFICYNHELNNVTPLPEQLGPNTSSSDILELISSKDLAHQMTDNDWNLFKSIHQVELIYHTFGKQRFRNATTANLERFMRRFNEVQFWVATEVCLCQEEERRALLLKKFIKLAAYLKEQKNLNSFFAVMFGLSNTAVSRLSRTWQKLPNKFRKLYSIFERLMDPSWNHRSYRLAVAKLSAPLIPFLPLILKDLTFLHEGNRSTLDSLVNFEKMRMIAKTVQMFHSCRSQAYIPLSPLRPRPVNILEDPSAARISACSEHSMTLRSPATTWAYIQHMKVIDNQKKLMELSRSLEPASST

Target ORF information:

RefSeq Version	XM_012957843.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 3 (rapgef3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957843.3

ORF Insert Sequence:

ATGAGAATAA TAGGGCAGCA GCTGCGGAAT CCCTGTGGTA TGAGGCACAG GAACAAGATG 
CACTTCTTCA GGACCTACTC CTATGAGATT CAGGTGGAGC AGAGATTGGA TATTCACAGC 
CGCATCCAAG GCATCAGATG GACACCGTTA CCTGATATTA AGTCTCAAGA AGAATACAAG 
CAGACTGTGA AACAGGTGGT AACACATAAA GTCTGGAGGG CAGCAAAAGT TTTGCACACT 
TACCTGCTTA CCGTGTCTCC AGCCATGATG CGTGACAGGA AACACCATTT CCGCAGCCAC 
CGACAATGCT GCTCAGGGAA GGAGCTTGTT GACTGGTTAC TGGGCATCAG TCATGGCCTG 
CAGTCCCGAA GCCAAGCCGT GGGGATATGG CAGGTGCTGG TGGATGAAGG AGCCCTTGTG 
CATGTGAGAC AAGAGCCCAA TTTCCATGAT AAAGACTCCC AATTTTATCG ATTCGTGAAA 
GCAGAACTAC ACGTTGACCC CTTGCTGACT GACAAAGATA CAGAAGAAGA AGTGCAAGAG 
TCCTTGGTAT TACTGTCTCA GTTGGGACCA GCAGCGTTGC TAACCATGAT TTTACGCAAA 
CCCCCTTGCC AGCGTACTGA AGAAGAGCTG GAAGTGATAT TTGAGGAGTT ACTTCACATC 
AAGGCGGTGT CCCATCTTTC CAACTCGGTG AAAAAGGAAT TGGCTTCTGT TTTGATATTT 
GAGTCTCATG CGAAAGCTGG TACAGTGTTG TTCAATCAAG GAGATAAGGG CACCTCCTGG 
TATATTATAT GGAAAGGTTC TGTAAACGTA ATTACACATG GGAAGGGCCT GGTTGCTACA 
CTTCACGAGG GAGATGATTT TGGGCAGTTG GCTCTTGTAA ATGATGCCCC AAGAGCTGCT 
ACCATAGTCT TGCGGGAAGA CAACTGCCAT TTTCTGCGTG TGGACAAGCA TGACTTCAAT 
CGCATTCTTC GGGATGTAGA AGCTAACACA GTTCGTCTGA AAGAACATGG GAAGGTGGTA 
TTAGTTTTGG AGAAAAATCC ACAAATATCA GCTGGAAACA ACCACCAACC AGCAGGGGCC 
TCCAACCAAA AGTACACTGT CATGTCTGGG ACAGCTGATA AAATCCTGGA ACACTTACTA 
GAGACTATAA AGCTGGATTC ATGCACAGAA GACTCCTTTG CAGGTGATTT CCTCTTGACT 
CACAGTATCT TCATGCCAAC AACCCAACTG TGCCAAGCTT TGCTGCACCA GTTCAACGTG 
GAGCCGTCAG AAGTGAATGA GCCAGAAAAG GCAACCTATT CCCTTCATAA GCGGCAGAAC 
ATCCTGCACC TGGTGAGCCT GTGGGTAGCT CTGTATGGGA AGTTACTGGA GGCTGAGCCA 
AGTGCCAAGA GCTTCCTAGA GAAACTTTCA GAACATGTCA GCAGAGATCC ACGGCTCCTT 
GCCTTTTGGA GAGAGAATCT TCAGGATAGG CGGAAAACCA GAACATTGGA GGTGAGCAGT 
GGGAATCACT CTCCCCAAAT AAAGATCAAC AGCTTTGGGA ACCTTTTTTC CAGTCTGGAG 
GAGGACGCAT CTAGTAACAC ATATACTATT CGAGCAGGAG ACAAAGTGCC TTATGATATT 
TATCGACCAG ACCACTCCTG CATCACCACA ATGCTGCCTG TGAATGCCTC GGTGCAAGAG 
GTGATATCAT CATTAGCAAC AAAAAATGGA TATAACAGAG ATCAAATCGT GGTAAAAGTC 
AACTCAAGTG GAGAAAGGGT CTGTCTAAAG CATGAGGACA CTGGCGTATT CACGTCTTTA 
GGGCTCAACG AAAGAATTTT CATCTGCTAC AATCATGAAC TGAACAATGT GACCCCCCTC 
CCTGAACAAC TGGGACCAAA CACCAGCAGC TCTGATATTC TGGAACTGAT CAGTTCAAAG 
GACCTGGCTC ACCAGATGAC GGATAATGAC TGGAACCTGT TTAAGAGCAT CCACCAAGTG 
GAGTTGATTT ATCACACGTT TGGGAAGCAG AGGTTTCGTA ACGCAACTAC TGCTAACCTT 
GAGCGGTTCA TGAGGCGATT CAATGAAGTT CAGTTCTGGG TGGCTACTGA GGTATGCCTA 
TGCCAAGAGG AGGAGCGGAG AGCACTGCTT CTAAAAAAGT TCATCAAACT CGCCGCTTAC 
CTCAAGGAGC AGAAAAATCT GAACTCATTC TTTGCAGTAA TGTTCGGTCT CAGTAACACA 
GCCGTCAGCC GCTTGTCCAG AACCTGGCAG AAACTGCCAA ACAAATTCAG GAAGCTATAT 
TCAATATTTG AGAGGCTCAT GGACCCCTCG TGGAACCATC GATCTTACAG ACTTGCTGTA 
GCAAAACTAA GTGCCCCTCT TATCCCATTT CTTCCACTTA TCCTTAAAGA CCTCACGTTC 
CTACATGAGG GAAACAGAAG CACATTGGAC AGCCTGGTAA ACTTTGAGAA AATGCGCATG 
ATTGCAAAGA CAGTTCAGAT GTTCCACAGC TGCCGCAGCC AGGCCTACAT TCCTCTCTCA 
CCTTTGAGAC CTCGACCTGT AAACATCCTT GAGGATCCTA GTGCTGCGCG GATATCTGCA 
TGTTCAGAGC ACTCAATGAC GTTAAGAAGC CCAGCTACAA CATGGGCTTA TATTCAGCAT 
ATGAAAGTGA TTGATAATCA GAAGAAACTG ATGGAATTGT CCCGTAGCCT GGAGCCTGCC 
TCTTCTACAT GA