nim1k cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nim1k
Entrez Gene ID	100491267
Full Name	NIM1 serine/threonine protein kinase
General protein information	Preferred Names NIM1 serine/threonine protein kinase Names serine/threonine-protein kinase NIM1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933034.4	XP_002933080.2	serine/threonine-protein kinase NIM1
XM_002933034.3	XP_002933080.2	serine/threonine-protein kinase NIM1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC471558	XP_001135819.1
Mus musculus (house mouse)	Nim1k	NP_780747.1
Danio rerio (zebrafish)	LOC100536336	XP_003199151.1
Caenorhabditis elegans (roundworm)	F49C5.4	NP_496679.3
Xenopus tropicalis (tropical clawed frog)	LOC100491267	XP_002933080.2
Homo sapiens (human)	NIM1K	NP_699192.1
	NIM1	XP_001091291.1
Bos taurus (cattle)	NIM1	NP_001192904.1
Gallus gallus (chicken)	NIM1	NP_001239059.1
Canis lupus familiaris (dog)	NIM1	XP_005619406.1
Rattus norvegicus (Norway rat)	Nim1	XP_006232044.1

Gene Ontology

The following nim1k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nim1k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03404	XM_002933034.4 Latest version!	Xenopus tropicalis NIM1 serine/threonine protein kinase (nim1k), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OXa03404	XM_002933034.3	Xenopus tropicalis NIM1 serine/threonine protein kinase (nim1k), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nim1k ( XM_002933034.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933080.2 Xenopus tropicalis NIM1 serine/threonine protein kinase (nim1k), mRNA.

Price & Availability↑

CloneID	OXa03404
Clone ID Related Accession (Same CDS sequence)	XM_002933034.4 , XM_002933034.3
Accession Version	XM_002933034.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase NIM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002933034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGACCA TGACGGCAAT GTACATGAAT GGAGGGGGGG TGGGCAATCC GCGGTATGCC 
CGGTGGAACC GGCAGGACAG CCTGGAGGAC TACGTCCGGT CCGAGTGCTA TGAGCAGGAT 
GCAGAAGGGA AGCAGCGCAA GCTCACGCCC TTCGAAAAGC TCACCCAAGA CATGTCACGT 
GATGAGAAGA CTGTCAAGGA ACTGACCCTG GGCAAGAGAA TTGGCCTTTA CCGAATGCGC 
GGAGAAATAG GGAGTGGCAA TTTCTCACAA GTGAAGCTCG GCATTCACGT CTTGACTAAA 
GAAAAAGTTG CTATAAAAAT CCTAGATAAA ACCAAGCTGG ACCAAAAAAC CCAGCGCCTG 
CTGTCCCGTG AGATCTCCAG CATGGAGAAG TTGCACCACC CCAACGTGAT CAGACTCTAT 
GAAGTTGTGG AGACCTTGTC TAAGCTGCAC CTTGTGATGG AGTATGCGGC GGGCGGTGAG 
CTCTTTGCCA AAATAAACAC TGAGGGGAAA TTATCGGAAT CAGAAAGTAA AGTCATCTTC 
TCTCAGATAC TGTCTGCTGT CAAGCACATG CACGACAACC AAATCATCCA CAGGGACCTT 
AAAGCTGAAA ATGTATTCTA TACCAGCAAC ACCTGTGTAA AGGTGGGAGA CTTTGGATTC 
AGCACGATAA GCAAAAAAGA TGAAACCCTG AACACTTTTT GTGGTTCTCC GCCCTATGCC 
GCCCCTGAGC TTTTTCGTGA TGAACACTAC GTTGGTGTTT TTGTTGATAT CTGGGCTTTG 
GGCATACTCC TGTACTTCAT GGTGAGTGGC AGTATGCCAT TCCGAGCAGA TACTGTGGCC 
AAACTGAAAA AGTGCATTCT TGATGGAACA TACACCATAC CTCCTTATGT GTCAGAACCG 
TGCCAGAAAC TAATCAAGGG GATGCTACAG CCTATCCCTT CGGACAGGTG CAGCATCGAT 
TACATCATAA ACAGCGAATG GATGAATGGG ATACAATACC CCAAGCCATT GGAACCACTA 
AAACTAGATC CCAAACATCT CTTTGATGGA ACTCCACTGA AGGACGAAGA CTCAGAGGTG 
AAGAACATTT TAGATAATTT GGGCATCACA GAAGAACATA TTATTAACAA TCGGGGAAAA 
GACTCCCGCA GCTCCATAAC TGGAATATAC AGGATCATTC TTCACAGGGT CCAGAAGAAA 
AGAGCGGTGG AGAGTGTCCC AATCATCACA AACCCAGAAC CAAAAGAAAA GTACATGAAG 
AGGCCTCACA GAACACACCG GGAAATAAGG CATACCTCTA AATTTTGTAC CATTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933080.2
CDS	1738..3057
Translation	MLTMTAMYMNGGGVGNPRYARWNRQDSLEDYVRSECYEQDAEGKQRKLTPFEKLTQDMSRDEKTVKELTLGKRIGLYRMRGEIGSGNFSQVKLGIHVLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNVIRLYEVVETLSKLHLVMEYAAGGELFAKINTEGKLSESESKVIFSQILSAVKHMHDNQIIHRDLKAENVFYTSNTCVKVGDFGFSTISKKDETLNTFCGSPPYAAPELFRDEHYVGVFVDIWALGILLYFMVSGSMPFRADTVAKLKKCILDGTYTIPPYVSEPCQKLIKGMLQPIPSDRCSIDYIINSEWMNGIQYPKPLEPLKLDPKHLFDGTPLKDEDSEVKNILDNLGITEEHIINNRGKDSRSSITGIYRIILHRVQKKRAVESVPIITNPEPKEKYMKRPHRTHREIRHTSKFCTIL

Target ORF information:

RefSeq Version	XM_002933034.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NIM1 serine/threonine protein kinase (nim1k), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933034.3

ORF Insert Sequence:

ATGTTGACCA TGACGGCAAT GTACATGAAT GGAGGGGGGG TGGGCAATCC GCGGTATGCC 
CGGTGGAACC GGCAGGACAG CCTGGAGGAC TACGTCCGGT CCGAGTGCTA TGAGCAGGAT 
GCAGAAGGGA AGCAGCGCAA GCTCACGCCC TTCGAAAAGC TCACCCAAGA CATGTCACGT 
GATGAGAAGA CTGTCAAGGA ACTGACCCTG GGCAAGAGAA TTGGCCTTTA CCGAATGCGC 
GGAGAAATAG GGAGTGGCAA TTTCTCACAA GTGAAGCTCG GCATTCACGT CTTGACTAAA 
GAAAAAGTTG CTATAAAAAT CCTAGATAAA ACCAAGCTGG ACCAAAAAAC CCAGCGCCTG 
CTGTCCCGTG AGATCTCCAG CATGGAGAAG TTGCACCACC CCAACGTGAT CAGACTCTAT 
GAAGTTGTGG AGACCTTGTC TAAGCTGCAC CTTGTGATGG AGTATGCGGC GGGCGGTGAG 
CTCTTTGCCA AAATAAACAC TGAGGGGAAA TTATCGGAAT CAGAAAGTAA AGTCATCTTC 
TCTCAGATAC TGTCTGCTGT CAAGCACATG CACGACAACC AAATCATCCA CAGGGACCTT 
AAAGCTGAAA ATGTATTCTA TACCAGCAAC ACCTGTGTAA AGGTGGGAGA CTTTGGATTC 
AGCACGATAA GCAAAAAAGA TGAAACCCTG AACACTTTTT GTGGTTCTCC GCCCTATGCC 
GCCCCTGAGC TTTTTCGTGA TGAACACTAC GTTGGTGTTT TTGTTGATAT CTGGGCTTTG 
GGCATACTCC TGTACTTCAT GGTGAGTGGC AGTATGCCAT TCCGAGCAGA TACTGTGGCC 
AAACTGAAAA AGTGCATTCT TGATGGAACA TACACCATAC CTCCTTATGT GTCAGAACCG 
TGCCAGAAAC TAATCAAGGG GATGCTACAG CCTATCCCTT CGGACAGGTG CAGCATCGAT 
TACATCATAA ACAGCGAATG GATGAATGGG ATACAATACC CCAAGCCATT GGAACCACTA 
AAACTAGATC CCAAACATCT CTTTGATGGA ACTCCACTGA AGGACGAAGA CTCAGAGGTG 
AAGAACATTT TAGATAATTT GGGCATCACA GAAGAACATA TTATTAACAA TCGGGGAAAA 
GACTCCCGCA GCTCCATAAC TGGAATATAC AGGATCATTC TTCACAGGGT CCAGAAGAAA 
AGAGCGGTGG AGAGTGTCCC AATCATCACA AACCCAGAAC CAAAAGAAAA GTACATGAAG 
AGGCCTCACA GAACACACCG GGAAATAAGG CATACCTCTA AATTTTGTAC CATTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nim1k ( XM_002933034.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933080.2 Xenopus tropicalis NIM1 serine/threonine protein kinase (nim1k), mRNA.

Price & Availability↑

CloneID	OXa03404
Clone ID Related Accession (Same CDS sequence)	XM_002933034.4 , XM_002933034.3
Accession Version	XM_002933034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase NIM1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGACCA TGACGGCAAT GTACATGAAT GGAGGGGGGG TGGGCAATCC GCGGTATGCC 
CGGTGGAACC GGCAGGACAG CCTGGAGGAC TACGTCCGGT CCGAGTGCTA TGAGCAGGAT 
GCAGAAGGGA AGCAGCGCAA GCTCACGCCC TTCGAAAAGC TCACCCAAGA CATGTCACGT 
GATGAGAAGA CTGTCAAGGA ACTGACCCTG GGCAAGAGAA TTGGCCTTTA CCGAATGCGC 
GGAGAAATAG GGAGTGGCAA TTTCTCACAA GTGAAGCTCG GCATTCACGT CTTGACTAAA 
GAAAAAGTTG CTATAAAAAT CCTAGATAAA ACCAAGCTGG ACCAAAAAAC CCAGCGCCTG 
CTGTCCCGTG AGATCTCCAG CATGGAGAAG TTGCACCACC CCAACGTGAT CAGACTCTAT 
GAAGTTGTGG AGACCTTGTC TAAGCTGCAC CTTGTGATGG AGTATGCGGC GGGCGGTGAG 
CTCTTTGCCA AAATAAACAC TGAGGGGAAA TTATCGGAAT CAGAAAGTAA AGTCATCTTC 
TCTCAGATAC TGTCTGCTGT CAAGCACATG CACGACAACC AAATCATCCA CAGGGACCTT 
AAAGCTGAAA ATGTATTCTA TACCAGCAAC ACCTGTGTAA AGGTGGGAGA CTTTGGATTC 
AGCACGATAA GCAAAAAAGA TGAAACCCTG AACACTTTTT GTGGTTCTCC GCCCTATGCC 
GCCCCTGAGC TTTTTCGTGA TGAACACTAC GTTGGTGTTT TTGTTGATAT CTGGGCTTTG 
GGCATACTCC TGTACTTCAT GGTGAGTGGC AGTATGCCAT TCCGAGCAGA TACTGTGGCC 
AAACTGAAAA AGTGCATTCT TGATGGAACA TACACCATAC CTCCTTATGT GTCAGAACCG 
TGCCAGAAAC TAATCAAGGG GATGCTACAG CCTATCCCTT CGGACAGGTG CAGCATCGAT 
TACATCATAA ACAGCGAATG GATGAATGGG ATACAATACC CCAAGCCATT GGAACCACTA 
AAACTAGATC CCAAACATCT CTTTGATGGA ACTCCACTGA AGGACGAAGA CTCAGAGGTG 
AAGAACATTT TAGATAATTT GGGCATCACA GAAGAACATA TTATTAACAA TCGGGGAAAA 
GACTCCCGCA GCTCCATAAC TGGAATATAC AGGATCATTC TTCACAGGGT CCAGAAGAAA 
AGAGCGGTGG AGAGTGTCCC AATCATCACA AACCCAGAAC CAAAAGAAAA GTACATGAAG 
AGGCCTCACA GAACACACCG GGAAATAAGG CATACCTCTA AATTTTGTAC CATTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933080.2
CDS	1587..2906
Translation	MLTMTAMYMNGGGVGNPRYARWNRQDSLEDYVRSECYEQDAEGKQRKLTPFEKLTQDMSRDEKTVKELTLGKRIGLYRMRGEIGSGNFSQVKLGIHVLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNVIRLYEVVETLSKLHLVMEYAAGGELFAKINTEGKLSESESKVIFSQILSAVKHMHDNQIIHRDLKAENVFYTSNTCVKVGDFGFSTISKKDETLNTFCGSPPYAAPELFRDEHYVGVFVDIWALGILLYFMVSGSMPFRADTVAKLKKCILDGTYTIPPYVSEPCQKLIKGMLQPIPSDRCSIDYIINSEWMNGIQYPKPLEPLKLDPKHLFDGTPLKDEDSEVKNILDNLGITEEHIINNRGKDSRSSITGIYRIILHRVQKKRAVESVPIITNPEPKEKYMKRPHRTHREIRHTSKFCTIL

Target ORF information:

RefSeq Version	XM_002933034.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NIM1 serine/threonine protein kinase (nim1k), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933034.4

ORF Insert Sequence:

ATGTTGACCA TGACGGCAAT GTACATGAAT GGAGGGGGGG TGGGCAATCC GCGGTATGCC 
CGGTGGAACC GGCAGGACAG CCTGGAGGAC TACGTCCGGT CCGAGTGCTA TGAGCAGGAT 
GCAGAAGGGA AGCAGCGCAA GCTCACGCCC TTCGAAAAGC TCACCCAAGA CATGTCACGT 
GATGAGAAGA CTGTCAAGGA ACTGACCCTG GGCAAGAGAA TTGGCCTTTA CCGAATGCGC 
GGAGAAATAG GGAGTGGCAA TTTCTCACAA GTGAAGCTCG GCATTCACGT CTTGACTAAA 
GAAAAAGTTG CTATAAAAAT CCTAGATAAA ACCAAGCTGG ACCAAAAAAC CCAGCGCCTG 
CTGTCCCGTG AGATCTCCAG CATGGAGAAG TTGCACCACC CCAACGTGAT CAGACTCTAT 
GAAGTTGTGG AGACCTTGTC TAAGCTGCAC CTTGTGATGG AGTATGCGGC GGGCGGTGAG 
CTCTTTGCCA AAATAAACAC TGAGGGGAAA TTATCGGAAT CAGAAAGTAA AGTCATCTTC 
TCTCAGATAC TGTCTGCTGT CAAGCACATG CACGACAACC AAATCATCCA CAGGGACCTT 
AAAGCTGAAA ATGTATTCTA TACCAGCAAC ACCTGTGTAA AGGTGGGAGA CTTTGGATTC 
AGCACGATAA GCAAAAAAGA TGAAACCCTG AACACTTTTT GTGGTTCTCC GCCCTATGCC 
GCCCCTGAGC TTTTTCGTGA TGAACACTAC GTTGGTGTTT TTGTTGATAT CTGGGCTTTG 
GGCATACTCC TGTACTTCAT GGTGAGTGGC AGTATGCCAT TCCGAGCAGA TACTGTGGCC 
AAACTGAAAA AGTGCATTCT TGATGGAACA TACACCATAC CTCCTTATGT GTCAGAACCG 
TGCCAGAAAC TAATCAAGGG GATGCTACAG CCTATCCCTT CGGACAGGTG CAGCATCGAT 
TACATCATAA ACAGCGAATG GATGAATGGG ATACAATACC CCAAGCCATT GGAACCACTA 
AAACTAGATC CCAAACATCT CTTTGATGGA ACTCCACTGA AGGACGAAGA CTCAGAGGTG 
AAGAACATTT TAGATAATTT GGGCATCACA GAAGAACATA TTATTAACAA TCGGGGAAAA 
GACTCCCGCA GCTCCATAAC TGGAATATAC AGGATCATTC TTCACAGGGT CCAGAAGAAA 
AGAGCGGTGG AGAGTGTCCC AATCATCACA AACCCAGAAC CAAAAGAAAA GTACATGAAG 
AGGCCTCACA GAACACACCG GGAAATAAGG CATACCTCTA AATTTTGTAC CATTTTATAA