mical3 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012959374.2	XP_012814828.1	protein-methionine sulfoxide oxidase MICAL3 isoform X5
XM_012959375.2	XP_012814829.1	protein-methionine sulfoxide oxidase MICAL3 isoform X6
XM_018089442.1	XP_017944931.1	protein-methionine sulfoxide oxidase MICAL3 isoform X7
XM_012959380.2	XP_012814834.1	protein-methionine sulfoxide oxidase MICAL3 isoform X8
XM_012959383.2	XP_012814837.1	protein-methionine sulfoxide oxidase MICAL3 isoform X11
XM_031898575.1	XP_031754435.1	F-actin-monooxygenase MICAL3 isoform X2
XM_012959365.3	XP_012814819.2	F-actin-monooxygenase MICAL3 isoform X5
XM_031898578.1	XP_031754438.1	F-actin-monooxygenase MICAL3 isoform X6
XM_031898579.1	XP_031754439.1	F-actin-monooxygenase MICAL3 isoform X7
XM_031898580.1	XP_031754440.1	F-actin-monooxygenase MICAL3 isoform X8
XM_031898584.1	XP_031754444.1	F-actin-monooxygenase MICAL3 isoform X14
XM_012959375.3	XP_012814829.2	F-actin-monooxygenase MICAL3 isoform X15
XM_031898585.1	XP_031754445.1	F-actin-monooxygenase MICAL3 isoform X16
XM_031898586.1	XP_031754446.1	F-actin-monooxygenase MICAL3 isoform X17
XM_031898589.1	XP_031754449.1	F-actin-monooxygenase MICAL3 isoform X20
XM_012959367.2	XP_012814821.1	protein-methionine sulfoxide oxidase MICAL3 isoform X2
XM_012959373.2	XP_012814827.1	protein-methionine sulfoxide oxidase MICAL3 isoform X4
XM_031898581.1	XP_031754441.1	F-actin-monooxygenase MICAL3 isoform X9
XM_012959371.3	XP_012814825.2	F-actin-monooxygenase MICAL3 isoform X13
XM_031898587.1	XP_031754447.1	F-actin-monooxygenase MICAL3 isoform X18
XM_012959382.3	XP_012814836.1	F-actin-monooxygenase MICAL3 isoform X21
XM_012959365.2	XP_012814819.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_012959371.2	XP_012814825.1	protein-methionine sulfoxide oxidase MICAL3 isoform X3
XM_012959382.2	XP_012814836.1	protein-methionine sulfoxide oxidase MICAL3 isoform X10
XM_012959381.2	XP_012814835.1	protein-methionine sulfoxide oxidase MICAL3 isoform X9
XM_031898588.1	XP_031754448.1	F-actin-monooxygenase MICAL3 isoform X19
XM_031898573.1	XP_031754433.1	F-actin-monooxygenase MICAL3 isoform X1
XM_031898574.1	XP_031754434.1	F-actin-monooxygenase MICAL3 isoform X1
XM_031898576.1	XP_031754436.1	F-actin-monooxygenase MICAL3 isoform X3
XM_031898577.1	XP_031754437.1	F-actin-monooxygenase MICAL3 isoform X4
XM_012959367.3	XP_012814821.2	F-actin-monooxygenase MICAL3 isoform X10
XM_031898582.1	XP_031754442.1	F-actin-monooxygenase MICAL3 isoform X11
XM_031898583.1	XP_031754443.1	F-actin-monooxygenase MICAL3 isoform X12
XM_031898590.1	XP_031754450.1	F-actin-monooxygenase MICAL3 isoform X22
XM_031898572.1	XP_031754432.1	F-actin-monooxygenase MICAL3 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa54226	XM_012959382.3 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa33568	XM_012959382.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa54206	XM_031898573.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54206	XM_031898572.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54206	XM_031898574.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54207	XM_031898575.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54208	XM_031898576.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54209	XM_031898577.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54210	XM_012959365.3 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54211	XM_031898578.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54212	XM_031898579.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54213	XM_031898580.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54214	XM_031898581.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54215	XM_012959367.3 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54216	XM_031898582.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54217	XM_031898583.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54218	XM_012959371.3 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54219	XM_031898584.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54220	XM_012959375.3 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54221	XM_031898585.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54222	XM_031898586.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54223	XM_031898587.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54224	XM_031898588.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54225	XM_031898589.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa54227	XM_031898590.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa33553	XM_012959365.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa33554	XM_012959367.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa33557	XM_012959371.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa33559	XM_012959373.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa33560	XM_012959374.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa33561	XM_012959375.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa33566	XM_012959380.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa33567	XM_012959381.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa33569	XM_012959383.2 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa40692	XM_018089442.1 Latest version!	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

mical3 ( XM_012959382.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814836.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OXa54226
Clone ID Related Accession (Same CDS sequence)	XM_012959382.3
Accession Version	XM_012959382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC ACTAGTTCTG CCGTTTCCTA TCAGCCATTG 
CAGAGGATGC TGGGAGTTGT AACCGAACAA GCCGGACAGT CAGAGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814836.1
CDS	343..3453
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGTSSAVSYQPLQRMLGVVTEQAGQSEF

Target ORF information:

RefSeq Version	XM_012959382.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959382.3

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC ACTAGTTCTG CCGTTTCCTA TCAGCCATTG 
CAGAGGATGC TGGGAGTTGT AACCGAACAA GCCGGACAGT CAGAGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959382.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814836.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X10, mRNA.

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CloneID	OXa33568
Clone ID Related Accession (Same CDS sequence)	XM_012959382.2
Accession Version	XM_012959382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC ACTAGTTCTG CCGTTTCCTA TCAGCCATTG 
CAGAGGATGC TGGGAGTTGT AACCGAACAA GCCGGACAGT CAGAGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814836.1
CDS	396..3506
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGTSSAVSYQPLQRMLGVVTEQAGQSEF

Target ORF information:

RefSeq Version	XM_012959382.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959382.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC ACTAGTTCTG CCGTTTCCTA TCAGCCATTG 
CAGAGGATGC TGGGAGTTGT AACCGAACAA GCCGGACAGT CAGAGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898573.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754433.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54206
Clone ID Related Accession (Same CDS sequence)	XM_031898573.1 , XM_031898574.1 , XM_031898572.1
Accession Version	XM_031898573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGTTGATC CCGTTGCCGG GACTCCGCGC CGTAGACCTC TCTCCTTCTG CCCCTGCTGT 
GCCCACGAAG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754433.1
CDS	256..6984
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898573.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898573.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGTTGATC CCGTTGCCGG GACTCCGCGC CGTAGACCTC TCTCCTTCTG CCCCTGCTGT 
GCCCACGAAG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898574.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754434.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X3, mRNA.

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CloneID	OXa54206
Clone ID Related Accession (Same CDS sequence)	XM_031898573.1 , XM_031898574.1 , XM_031898572.1
Accession Version	XM_031898574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGTTGATC CCGTTGCCGG GACTCCGCGC CGTAGACCTC TCTCCTTCTG CCCCTGCTGT 
GCCCACGAAG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754434.1
CDS	405..7133
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898574.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898574.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGTTGATC CCGTTGCCGG GACTCCGCGC CGTAGACCTC TCTCCTTCTG CCCCTGCTGT 
GCCCACGAAG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898572.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754432.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X1, mRNA.

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CloneID	OXa54206
Clone ID Related Accession (Same CDS sequence)	XM_031898573.1 , XM_031898574.1 , XM_031898572.1
Accession Version	XM_031898572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGTTGATC CCGTTGCCGG GACTCCGCGC CGTAGACCTC TCTCCTTCTG CCCCTGCTGT 
GCCCACGAAG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754432.1
CDS	359..7087
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898572.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898572.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGTTGATC CCGTTGCCGG GACTCCGCGC CGTAGACCTC TCTCCTTCTG CCCCTGCTGT 
GCCCACGAAG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898575.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754435.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X4, mRNA.

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CloneID	OXa54207
Clone ID Related Accession (Same CDS sequence)	XM_031898575.1
Accession Version	XM_031898575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGTATA TAAAGATGTA CACAGGTGGA 
GTGAGCTCAT TGGCTGAGCA AATAGCCAAT CAGCTTCAAA AGAAGGAAAC GCCAAAACCT 
CTGTTCGGGA AGAAAGTGGG CTCATTTAGG AAAGAGTTCC CCCAGAACCT GGGAGGGAGC 
GACGTCTGCT ATTTCTGCCG CCGGAGGGTT TACGTCATGG AGCGGCTCAG CGCCGAGGGC 
AAGTTCTTCC ACCGGAGCTG CTTTAAGTGT GACTACTGTA GCACAACGCT GCGCCTCTCA 
TGTTATGCCT ATGACCTTGA GGATGGAAAA TTTTACTGCA AACCTCACTA CTGTTACCGC 
CGGTCCGGAT ACAGCCAGAG GAAGAGGCCG GCCATGCCTG CACTTTCGGG AAAAGAAGTC 
ACAAGGACTG TGGAAGACAC CCCCGACGCG GAAGACCAAG GACGAGCCCC GCTGCTGCCC 
GCCCCCGCTG CTGAGAAGAC CCCAGGCCCC AGTGTGAACG GCGTGGAAGA TCACAGCGCC 
GCCAAGAGGC TGAGAGCCAC CCCAGAGCGC ATTGAGCTTG AGAATTACCG CGTCTCCCTG 
CAGAAGGAGG AGCTGGAGGA GGTGCCCGAG GAAACCCTGG CGGAGCATAA TCTGAGCAGC 
CTATTGGATA AAGGGGAGGT GGAAGAGGTG GCCAGCAGCA GTTCCGAGTC CGAGATGGAG 
GAGGAGGAGG ACGAAGAAGA AGAGGAGGAG GAGCCAGGAG AGTGCCTTCC CCCCTCAGAT 
CTGGGTGGGG TGCCGTGGAA AGAGGCCGTC AAGATCCACG CCAAGTTAAA GGGTCAGGAC 
ATAGATGTGG AGGTTGTGGG AACCCAGGGG GCTGGTGGCA GTGAAGAGGA GGAGGAGGAG 
GAGGAAGAAG AGGACGAAGA GGAGGAAGAT GAAGAGGTAT CAAGTGAAGA GGGTGAGTAC 
TGCCCATGGG AGAGAGAGCT TCAGCAGGGC CTGTGGATAC AGCGTATCTC TGATGAAGAT 
ACAGTTGGTA TATTTAAAGC CGGGAACCTC AGACTCCAGC AAGCCCTCTC CCCTGTTGAC 
CCCACGGAAA TAAAGGCAGA CGTGCGCTGG ACACACGTCC GCGAGGTTAA GGAAGAGCGG 
AGAGGGTCTA AGGAAGAGAG GGGAGAAGTG AGCATGTCCT CTCCTTCCAA AGCGCGTCAT 
CTCCATTCCC ACCGTCACGA CCCAGTGAGA GCGTGGCTGG AGTCCGTATC AGGAGCTACT 
GGCGAGTATG AAGAAGGTGA GGAGGAGGAG CTGGGTACTT GCCTGGCGCA GGCTGAAGCA 
GCTGGTGATG GGGACACCTC CGCAGACCTT GGTGATGATG ATGACATCCC GTCCGACGCC 
GAGGCCGAAG AACGGCTCCA GCAGTCTCAG AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA 
GAGCCTAAGG TGGAGCAACC CGTCCTGCCA GCAATTGCAG ATTCTGAGAG CGTCCCCAGC 
CCAGTCTCTC TGGCACCGAA AGCCCCCGTG CTGTCTCCAA GAAGGACACT CAAAGCGGAA 
GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG GCAGTGACCT CTCCTGGGGG TCGGTTCTTT 
GCGGATCCGT ATCTGCCATT GGACAGCAGC AACAAAGCCT TAGTGGAAGC AAAAACTCCA 
ACTCCTCTAT CTCCTGTTTG CTCCCAACCA GTCCCATCGC AAGAACCTGT TATCCCTACA 
TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA AACACGCGCC CACTTATATC CCCATCAGCT 
CCTATTTGTT CCCAGCCACT GCCGTCCTCA GAGGGTACCC TAAATGCTAC AGACAGGCCC 
CCCGTGCGTC AGCAGCCATC CCCTCTAATC CCCTCTACTT CTTTAACCAA ACTGCCTTTT 
ACTGGTCACG TCATGGGATC TGAACAATTA GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA 
CGTATTGACC TTATCGAAGA GTTCTGGACA AAAAGCGCCG AGATCAGGCG CAGCTTAGGG 
CTGGCCCCCG TGGACAGAGA TCGGGTACAT GAGATCAGTT TCCCTGCCCT AGCTTCAACT 
CCTCTTCAGC CTCAGGCACC TAAATCCAAA CCTGAGGAGC AAAAGTCACC GTCTCTGAAG 
CCACAACAAG TTGCAAGAAG AGTGAACCCT TCCCGGCTGG ACATTGAACT GCTCTCCCCT 
CTGACTCCAA TATCCCCTTC TGAGAAGGAG CTGAAGAGCT CAGATGGTGA GAAGAAGGAT 
ATCTCCACCA GCTCTGGCTT AGGCCTGAAT GGCAGTTCCA TAACACGTAC TGCCGCCAGT 
GAAAGCTTTA ACACCTCTGA TTCAGCCATG CTTACTCCAC CATCCAGCCC TCCACCACCT 
CCTCCCCAAG GGGAAGAGCC AGCTACTCTA CGTAGAAGCC GTACTCCTTG CCAGAATGGT 
CCAGAATCTA TCCCAGCCCC TGCCCTAGCA CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC 
CCCCGCTGGG AAGAATCTGT ACGTAAGTCA TTTGTAGAAA GTGTAGACGA GATCCCCTTT 
GCCGATGATG TGGAGGACAC GTACGACGAA AGAACGGAGA ATTCCAGCCT TCAAGAGAGG 
TTCTTTACCC CACCAACGAG TCGGGTTAAA CCACATAGAA TTGGTTTCTC CAAGGAAAAT 
GGAGAGATCC CATCTTCTTC GGAGAGAGTG CACCACAGAA AAAGGCTCTT GCCCGACGTC 
ACTCAGGAGG CCAAAGAACG GGCAGAGGAG CGGATGAAAG CCCGGGAGAA GTCTGTGAAG 
AGCCAGGCAC TGAAAGACGC CATAGCACAA CAGCGGCACA AAATGAAACA ACTAGAACCA 
GGGGCAAGAT CGGCCAAAGC TCTGCCATCT ACCGTTATGT TCAGCGATTA CCCTGCAGTA 
TCTCCTCTCC TGAGAACCCA GACCGAGACT CCCACCCTGA AGCTGGATAC CGACCAGGAG 
GTCTTTATAA CCCCCCCAGC AGCTCCTGAC GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC 
GGGAAACCCA AGAAAAGGCC GTCTATATTC TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG 
GGGGAAAGCA GTCGGGCGTC TGATAAACAG GCGACGGACG AGAGCACCAA ACCCAAGTCC 
TTGTGGAAAT CCGTCTTCTC CGGGTATAAG AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG 
GAGGACAAGT CTACTCCGAG CACTCCATCC AGCAGCACCA CCGTGGACTC TGGGAAAGCC 
AAGGTGGCAA ATATGATCCG CGGAGCAGAC CTGTCCCTCC GACGTCACCT GAGCTTCTCG 
GAGGACTCGG ACCTGTCCAG CGACGATGTC TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA 
GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC CCTGAATTTC ACATGCACAG AGATGAGTCG 
GTGTATGTCC CCCACGCCTT GGCATTCAGA CGGTCATACT CATCAAAGAA AACCTACACG 
GACGAGGAGC TAAGCGCTAA GCTAACAAGG CGGGTGCAGA AAGCGGCACG GAGACAGGCC 
AAGCAGGAGG AGCTAAAGCG GCTGCATCGG GCACAGATCA TTCAGCGCCA GCTGGAACAA 
GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG 
GGAGAAGCAG ACTATTGGGG AGAGTCTTAT TACAGTGACC TCATCGATTT GCACCTGGGT 
GTTGATCCCG TTGCCGGGAC TCCGCGCCGT AGACCTCTCT CCTTCTGCCC CTGCTGTGCC 
CACGAAGGGA TGGGAAAGAA GGACGACCCG AAACTCATGC AGGAATGGTT CCGCCTGGTG 
CAGGAGAAGA ACGCGCTGGT GCGATACGAA TCGGAGCTGA TGATATTTGC CCGCGAGTTG 
GAGTTGGAGG ACAGACAGAG CCGACTTCAG CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA 
GATCAATTGA AGACGGAGCA GGAGCTGACG GACGAGAAGA AGATCCTGAA CGAGATGCTG 
GACGTGGTGG AACAGAGAGA TGCTCTGGTG GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG 
AAAGAGGAGG ACAAAGATCT GGAGGAGGTC ATGTTATCCA AGGGGTTTAA CCTCAACTGG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754435.1
CDS	359..7084
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898575.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898575.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGTATA TAAAGATGTA CACAGGTGGA 
GTGAGCTCAT TGGCTGAGCA AATAGCCAAT CAGCTTCAAA AGAAGGAAAC GCCAAAACCT 
CTGTTCGGGA AGAAAGTGGG CTCATTTAGG AAAGAGTTCC CCCAGAACCT GGGAGGGAGC 
GACGTCTGCT ATTTCTGCCG CCGGAGGGTT TACGTCATGG AGCGGCTCAG CGCCGAGGGC 
AAGTTCTTCC ACCGGAGCTG CTTTAAGTGT GACTACTGTA GCACAACGCT GCGCCTCTCA 
TGTTATGCCT ATGACCTTGA GGATGGAAAA TTTTACTGCA AACCTCACTA CTGTTACCGC 
CGGTCCGGAT ACAGCCAGAG GAAGAGGCCG GCCATGCCTG CACTTTCGGG AAAAGAAGTC 
ACAAGGACTG TGGAAGACAC CCCCGACGCG GAAGACCAAG GACGAGCCCC GCTGCTGCCC 
GCCCCCGCTG CTGAGAAGAC CCCAGGCCCC AGTGTGAACG GCGTGGAAGA TCACAGCGCC 
GCCAAGAGGC TGAGAGCCAC CCCAGAGCGC ATTGAGCTTG AGAATTACCG CGTCTCCCTG 
CAGAAGGAGG AGCTGGAGGA GGTGCCCGAG GAAACCCTGG CGGAGCATAA TCTGAGCAGC 
CTATTGGATA AAGGGGAGGT GGAAGAGGTG GCCAGCAGCA GTTCCGAGTC CGAGATGGAG 
GAGGAGGAGG ACGAAGAAGA AGAGGAGGAG GAGCCAGGAG AGTGCCTTCC CCCCTCAGAT 
CTGGGTGGGG TGCCGTGGAA AGAGGCCGTC AAGATCCACG CCAAGTTAAA GGGTCAGGAC 
ATAGATGTGG AGGTTGTGGG AACCCAGGGG GCTGGTGGCA GTGAAGAGGA GGAGGAGGAG 
GAGGAAGAAG AGGACGAAGA GGAGGAAGAT GAAGAGGTAT CAAGTGAAGA GGGTGAGTAC 
TGCCCATGGG AGAGAGAGCT TCAGCAGGGC CTGTGGATAC AGCGTATCTC TGATGAAGAT 
ACAGTTGGTA TATTTAAAGC CGGGAACCTC AGACTCCAGC AAGCCCTCTC CCCTGTTGAC 
CCCACGGAAA TAAAGGCAGA CGTGCGCTGG ACACACGTCC GCGAGGTTAA GGAAGAGCGG 
AGAGGGTCTA AGGAAGAGAG GGGAGAAGTG AGCATGTCCT CTCCTTCCAA AGCGCGTCAT 
CTCCATTCCC ACCGTCACGA CCCAGTGAGA GCGTGGCTGG AGTCCGTATC AGGAGCTACT 
GGCGAGTATG AAGAAGGTGA GGAGGAGGAG CTGGGTACTT GCCTGGCGCA GGCTGAAGCA 
GCTGGTGATG GGGACACCTC CGCAGACCTT GGTGATGATG ATGACATCCC GTCCGACGCC 
GAGGCCGAAG AACGGCTCCA GCAGTCTCAG AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA 
GAGCCTAAGG TGGAGCAACC CGTCCTGCCA GCAATTGCAG ATTCTGAGAG CGTCCCCAGC 
CCAGTCTCTC TGGCACCGAA AGCCCCCGTG CTGTCTCCAA GAAGGACACT CAAAGCGGAA 
GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG GCAGTGACCT CTCCTGGGGG TCGGTTCTTT 
GCGGATCCGT ATCTGCCATT GGACAGCAGC AACAAAGCCT TAGTGGAAGC AAAAACTCCA 
ACTCCTCTAT CTCCTGTTTG CTCCCAACCA GTCCCATCGC AAGAACCTGT TATCCCTACA 
TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA AACACGCGCC CACTTATATC CCCATCAGCT 
CCTATTTGTT CCCAGCCACT GCCGTCCTCA GAGGGTACCC TAAATGCTAC AGACAGGCCC 
CCCGTGCGTC AGCAGCCATC CCCTCTAATC CCCTCTACTT CTTTAACCAA ACTGCCTTTT 
ACTGGTCACG TCATGGGATC TGAACAATTA GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA 
CGTATTGACC TTATCGAAGA GTTCTGGACA AAAAGCGCCG AGATCAGGCG CAGCTTAGGG 
CTGGCCCCCG TGGACAGAGA TCGGGTACAT GAGATCAGTT TCCCTGCCCT AGCTTCAACT 
CCTCTTCAGC CTCAGGCACC TAAATCCAAA CCTGAGGAGC AAAAGTCACC GTCTCTGAAG 
CCACAACAAG TTGCAAGAAG AGTGAACCCT TCCCGGCTGG ACATTGAACT GCTCTCCCCT 
CTGACTCCAA TATCCCCTTC TGAGAAGGAG CTGAAGAGCT CAGATGGTGA GAAGAAGGAT 
ATCTCCACCA GCTCTGGCTT AGGCCTGAAT GGCAGTTCCA TAACACGTAC TGCCGCCAGT 
GAAAGCTTTA ACACCTCTGA TTCAGCCATG CTTACTCCAC CATCCAGCCC TCCACCACCT 
CCTCCCCAAG GGGAAGAGCC AGCTACTCTA CGTAGAAGCC GTACTCCTTG CCAGAATGGT 
CCAGAATCTA TCCCAGCCCC TGCCCTAGCA CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC 
CCCCGCTGGG AAGAATCTGT ACGTAAGTCA TTTGTAGAAA GTGTAGACGA GATCCCCTTT 
GCCGATGATG TGGAGGACAC GTACGACGAA AGAACGGAGA ATTCCAGCCT TCAAGAGAGG 
TTCTTTACCC CACCAACGAG TCGGGTTAAA CCACATAGAA TTGGTTTCTC CAAGGAAAAT 
GGAGAGATCC CATCTTCTTC GGAGAGAGTG CACCACAGAA AAAGGCTCTT GCCCGACGTC 
ACTCAGGAGG CCAAAGAACG GGCAGAGGAG CGGATGAAAG CCCGGGAGAA GTCTGTGAAG 
AGCCAGGCAC TGAAAGACGC CATAGCACAA CAGCGGCACA AAATGAAACA ACTAGAACCA 
GGGGCAAGAT CGGCCAAAGC TCTGCCATCT ACCGTTATGT TCAGCGATTA CCCTGCAGTA 
TCTCCTCTCC TGAGAACCCA GACCGAGACT CCCACCCTGA AGCTGGATAC CGACCAGGAG 
GTCTTTATAA CCCCCCCAGC AGCTCCTGAC GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC 
GGGAAACCCA AGAAAAGGCC GTCTATATTC TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG 
GGGGAAAGCA GTCGGGCGTC TGATAAACAG GCGACGGACG AGAGCACCAA ACCCAAGTCC 
TTGTGGAAAT CCGTCTTCTC CGGGTATAAG AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG 
GAGGACAAGT CTACTCCGAG CACTCCATCC AGCAGCACCA CCGTGGACTC TGGGAAAGCC 
AAGGTGGCAA ATATGATCCG CGGAGCAGAC CTGTCCCTCC GACGTCACCT GAGCTTCTCG 
GAGGACTCGG ACCTGTCCAG CGACGATGTC TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA 
GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC CCTGAATTTC ACATGCACAG AGATGAGTCG 
GTGTATGTCC CCCACGCCTT GGCATTCAGA CGGTCATACT CATCAAAGAA AACCTACACG 
GACGAGGAGC TAAGCGCTAA GCTAACAAGG CGGGTGCAGA AAGCGGCACG GAGACAGGCC 
AAGCAGGAGG AGCTAAAGCG GCTGCATCGG GCACAGATCA TTCAGCGCCA GCTGGAACAA 
GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG 
GGAGAAGCAG ACTATTGGGG AGAGTCTTAT TACAGTGACC TCATCGATTT GCACCTGGGT 
GTTGATCCCG TTGCCGGGAC TCCGCGCCGT AGACCTCTCT CCTTCTGCCC CTGCTGTGCC 
CACGAAGGGA TGGGAAAGAA GGACGACCCG AAACTCATGC AGGAATGGTT CCGCCTGGTG 
CAGGAGAAGA ACGCGCTGGT GCGATACGAA TCGGAGCTGA TGATATTTGC CCGCGAGTTG 
GAGTTGGAGG ACAGACAGAG CCGACTTCAG CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA 
GATCAATTGA AGACGGAGCA GGAGCTGACG GACGAGAAGA AGATCCTGAA CGAGATGCTG 
GACGTGGTGG AACAGAGAGA TGCTCTGGTG GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG 
AAAGAGGAGG ACAAAGATCT GGAGGAGGTC ATGTTATCCA AGGGGTTTAA CCTCAACTGG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898576.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754436.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X5, mRNA.

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CloneID	OXa54208
Clone ID Related Accession (Same CDS sequence)	XM_031898576.1
Accession Version	XM_031898576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GTTCCGAGTC CGAGATGGAG 
GAGGAGGAGG ACGAAGAAGA AGAGGAGGAG GAGCCAGGAG AGTGCCTTCC CCCCTCAGAT 
CTGGGTGGGG TGCCGTGGAA AGAGGCCGTC AAGATCCACG CCAAGTTAAA GGGTCAGGAC 
ATAGATGTGG AGGTTGTGGG AACCCAGGGG GCTGGTGGCA GTGAAGAGGA GGAGGAGGAG 
GAGGAAGAAG AGGACGAAGA GGAGGAAGAT GAAGAGGTAT CAAGTGAAGA GGGTGAGTAC 
TGCCCATGGG AGAGAGAGCT TCAGCAGGGC CTGTGGATAC AGCGTATCTC TGATGAAGAT 
ACAGTTGGTA TATTTAAAGC CGGGAACCTC AGACTCCAGC AAGCCCTCTC CCCTGTTGAC 
CCCACGGAAA TAAAGGCAGA CGTGCGCTGG ACACACGTCC GCGAGGTTAA GGAAGAGCGG 
AGAGGGTCTA AGGAAGAGAG GGGAGAAGTG AGCATGTCCT CTCCTTCCAA AGCGCGTCAT 
CTCCATTCCC ACCGTCACGA CCCAGTGAGA GCGTGGCTGG AGTCCGTATC AGGAGCTACT 
GGCGAGTATG AAGAAGGTGA GGAGGAGGAG CTGGGTACTT GCCTGGCGCA GGCTGAAGCA 
GCTGGTGATG GGGACACCTC CGCAGACCTT GGTGATGATG ATGACATCCC GTCCGACGCC 
GAGGCCGAAG AACGGCTCCA GCAGTCTCAG AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA 
GAGCCTAAGG TGGAGCAACC CGTCCTGCCA GCAATTGCAG ATTCTGAGAG CGTCCCCAGC 
CCAGTCTCTC TGGCACCGAA AGCCCCCGTG CTGTCTCCAA GAAGGACACT CAAAGCGGAA 
GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG GCAGTGACCT CTCCTGGGGG TCGGTTCTTT 
GCGGATCCGT ATCTGCCATT GGACAGCAGC AACAAAGCCT TAGTGGAAGC AAAAACTCCA 
ACTCCTCTAT CTCCTGTTTG CTCCCAACCA GTCCCATCGC AAGAACCTGT TATCCCTACA 
TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA AACACGCGCC CACTTATATC CCCATCAGCT 
CCTATTTGTT CCCAGCCACT GCCGTCCTCA GAGGGTACCC TAAATGCTAC AGACAGGCCC 
CCCGTGCGTC AGCAGCCATC CCCTCTAATC CCCTCTACTT CTTTAACCAA ACTGCCTTTT 
ACTGGTCACG TCATGGGATC TGAACAATTA GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA 
CGTATTGACC TTATCGAAGA GTTCTGGACA AAAAGCGCCG AGATCAGGCG CAGCTTAGGG 
CTGGCCCCCG TGGACAGAGA TCGGGTACAT GAGATCAGTT TCCCTGCCCT AGCTTCAACT 
CCTCTTCAGC CTCAGGCACC TAAATCCAAA CCTGAGGAGC AAAAGTCACC GTCTCTGAAG 
CCACAACAAG TTGCAAGAAG AGTGAACCCT TCCCGGCTGG ACATTGAACT GCTCTCCCCT 
CTGACTCCAA TATCCCCTTC TGAGAAGGAG CTGAAGAGCT CAGATGGTGA GAAGAAGGAT 
ATCTCCACCA GCTCTGGCTT AGGCCTGAAT GGCAGTTCCA TAACACGTAC TGCCGCCAGT 
GAAAGCTTTA ACACCTCTGA TTCAGCCATG CTTACTCCAC CATCCAGCCC TCCACCACCT 
CCTCCCCAAG GGGAAGAGCC AGCTACTCTA CGTAGAAGCC GTACTCCTTG CCAGAATGGT 
CCAGAATCTA TCCCAGCCCC TGCCCTAGCA CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC 
CCCCGCTGGG AAGAATCTGT ACGTAAGTCA TTTGTAGAAA GTGTAGACGA GATCCCCTTT 
GCCGATGATG TGGAGGACAC GTACGACGAA AGAACGGAGA ATTCCAGCCT TCAAGAGAGG 
TTCTTTACCC CACCAACGAG TCGGGTTAAA CCACATAGAA TTGGTTTCTC CAAGGAAAAT 
GGAGAGATCC CATCTTCTTC GGAGAGAGTG CACCACAGAA AAAGGCTCTT GCCCGACGTC 
ACTCAGGAGG CCAAAGAACG GGCAGAGGAG CGGATGAAAG CCCGGGAGAA GTCTGTGAAG 
AGCCAGGCAC TGAAAGACGC CATAGCACAA CAGCGGCACA AAATGAAACA ACTAGAACCA 
GGGGCAAGAT CGGCCAAAGC TCTGCCATCT ACCGTTATGT TCAGCGATTA CCCTGCAGTA 
TCTCCTCTCC TGAGAACCCA GACCGAGACT CCCACCCTGA AGCTGGATAC CGACCAGGAG 
GTCTTTATAA CCCCCCCAGC AGCTCCTGAC GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC 
GGGAAACCCA AGAAAAGGCC GTCTATATTC TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG 
GGGGAAAGCA GTCGGGCGTC TGATAAACAG GCGACGGACG AGAGCACCAA ACCCAAGTCC 
TTGTGGAAAT CCGTCTTCTC CGGGTATAAG AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG 
GAGGACAAGT CTACTCCGAG CACTCCATCC AGCAGCACCA CCGTGGACTC TGGGAAAGCC 
AAGGTGGCAA ATATGATCCG CGGAGCAGAC CTGTCCCTCC GACGTCACCT GAGCTTCTCG 
GAGGACTCGG ACCTGTCCAG CGACGATGTC TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA 
GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC CCTGAATTTC ACATGCACAG AGATGAGTCG 
GTGTATGTCC CCCACGCCTT GGCATTCAGA CGGTCATACT CATCAAAGAA AACCTACACG 
GACGAGGAGC TAAGCGCTAA GCTAACAAGG CGGGTGCAGA AAGCGGCACG GAGACAGGCC 
AAGCAGGAGG AGCTAAAGCG GCTGCATCGG GCACAGATCA TTCAGCGCCA GCTGGAACAA 
GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG 
GGAGAAGCAG ACTATTGGGG AGAGTCTTAT TACAGTGACC TCATCGATTT GCACCTGGGT 
GTTGATCCCG TTGCCGGGAC TCCGCGCCGT AGACCTCTCT CCTTCTGCCC CTGCTGTGCC 
CACGAAGGGA TGGGAAAGAA GGACGACCCG AAACTCATGC AGGAATGGTT CCGCCTGGTG 
CAGGAGAAGA ACGCGCTGGT GCGATACGAA TCGGAGCTGA TGATATTTGC CCGCGAGTTG 
GAGTTGGAGG ACAGACAGAG CCGACTTCAG CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA 
GATCAATTGA AGACGGAGCA GGAGCTGACG GACGAGAAGA AGATCCTGAA CGAGATGCTG 
GACGTGGTGG AACAGAGAGA TGCTCTGGTG GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG 
AAAGAGGAGG ACAAAGATCT GGAGGAGGTC ATGTTATCCA AGGGGTTTAA CCTCAACTGG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754436.1
CDS	359..7084
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898576.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898576.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GTTCCGAGTC CGAGATGGAG 
GAGGAGGAGG ACGAAGAAGA AGAGGAGGAG GAGCCAGGAG AGTGCCTTCC CCCCTCAGAT 
CTGGGTGGGG TGCCGTGGAA AGAGGCCGTC AAGATCCACG CCAAGTTAAA GGGTCAGGAC 
ATAGATGTGG AGGTTGTGGG AACCCAGGGG GCTGGTGGCA GTGAAGAGGA GGAGGAGGAG 
GAGGAAGAAG AGGACGAAGA GGAGGAAGAT GAAGAGGTAT CAAGTGAAGA GGGTGAGTAC 
TGCCCATGGG AGAGAGAGCT TCAGCAGGGC CTGTGGATAC AGCGTATCTC TGATGAAGAT 
ACAGTTGGTA TATTTAAAGC CGGGAACCTC AGACTCCAGC AAGCCCTCTC CCCTGTTGAC 
CCCACGGAAA TAAAGGCAGA CGTGCGCTGG ACACACGTCC GCGAGGTTAA GGAAGAGCGG 
AGAGGGTCTA AGGAAGAGAG GGGAGAAGTG AGCATGTCCT CTCCTTCCAA AGCGCGTCAT 
CTCCATTCCC ACCGTCACGA CCCAGTGAGA GCGTGGCTGG AGTCCGTATC AGGAGCTACT 
GGCGAGTATG AAGAAGGTGA GGAGGAGGAG CTGGGTACTT GCCTGGCGCA GGCTGAAGCA 
GCTGGTGATG GGGACACCTC CGCAGACCTT GGTGATGATG ATGACATCCC GTCCGACGCC 
GAGGCCGAAG AACGGCTCCA GCAGTCTCAG AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA 
GAGCCTAAGG TGGAGCAACC CGTCCTGCCA GCAATTGCAG ATTCTGAGAG CGTCCCCAGC 
CCAGTCTCTC TGGCACCGAA AGCCCCCGTG CTGTCTCCAA GAAGGACACT CAAAGCGGAA 
GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG GCAGTGACCT CTCCTGGGGG TCGGTTCTTT 
GCGGATCCGT ATCTGCCATT GGACAGCAGC AACAAAGCCT TAGTGGAAGC AAAAACTCCA 
ACTCCTCTAT CTCCTGTTTG CTCCCAACCA GTCCCATCGC AAGAACCTGT TATCCCTACA 
TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA AACACGCGCC CACTTATATC CCCATCAGCT 
CCTATTTGTT CCCAGCCACT GCCGTCCTCA GAGGGTACCC TAAATGCTAC AGACAGGCCC 
CCCGTGCGTC AGCAGCCATC CCCTCTAATC CCCTCTACTT CTTTAACCAA ACTGCCTTTT 
ACTGGTCACG TCATGGGATC TGAACAATTA GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA 
CGTATTGACC TTATCGAAGA GTTCTGGACA AAAAGCGCCG AGATCAGGCG CAGCTTAGGG 
CTGGCCCCCG TGGACAGAGA TCGGGTACAT GAGATCAGTT TCCCTGCCCT AGCTTCAACT 
CCTCTTCAGC CTCAGGCACC TAAATCCAAA CCTGAGGAGC AAAAGTCACC GTCTCTGAAG 
CCACAACAAG TTGCAAGAAG AGTGAACCCT TCCCGGCTGG ACATTGAACT GCTCTCCCCT 
CTGACTCCAA TATCCCCTTC TGAGAAGGAG CTGAAGAGCT CAGATGGTGA GAAGAAGGAT 
ATCTCCACCA GCTCTGGCTT AGGCCTGAAT GGCAGTTCCA TAACACGTAC TGCCGCCAGT 
GAAAGCTTTA ACACCTCTGA TTCAGCCATG CTTACTCCAC CATCCAGCCC TCCACCACCT 
CCTCCCCAAG GGGAAGAGCC AGCTACTCTA CGTAGAAGCC GTACTCCTTG CCAGAATGGT 
CCAGAATCTA TCCCAGCCCC TGCCCTAGCA CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC 
CCCCGCTGGG AAGAATCTGT ACGTAAGTCA TTTGTAGAAA GTGTAGACGA GATCCCCTTT 
GCCGATGATG TGGAGGACAC GTACGACGAA AGAACGGAGA ATTCCAGCCT TCAAGAGAGG 
TTCTTTACCC CACCAACGAG TCGGGTTAAA CCACATAGAA TTGGTTTCTC CAAGGAAAAT 
GGAGAGATCC CATCTTCTTC GGAGAGAGTG CACCACAGAA AAAGGCTCTT GCCCGACGTC 
ACTCAGGAGG CCAAAGAACG GGCAGAGGAG CGGATGAAAG CCCGGGAGAA GTCTGTGAAG 
AGCCAGGCAC TGAAAGACGC CATAGCACAA CAGCGGCACA AAATGAAACA ACTAGAACCA 
GGGGCAAGAT CGGCCAAAGC TCTGCCATCT ACCGTTATGT TCAGCGATTA CCCTGCAGTA 
TCTCCTCTCC TGAGAACCCA GACCGAGACT CCCACCCTGA AGCTGGATAC CGACCAGGAG 
GTCTTTATAA CCCCCCCAGC AGCTCCTGAC GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC 
GGGAAACCCA AGAAAAGGCC GTCTATATTC TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG 
GGGGAAAGCA GTCGGGCGTC TGATAAACAG GCGACGGACG AGAGCACCAA ACCCAAGTCC 
TTGTGGAAAT CCGTCTTCTC CGGGTATAAG AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG 
GAGGACAAGT CTACTCCGAG CACTCCATCC AGCAGCACCA CCGTGGACTC TGGGAAAGCC 
AAGGTGGCAA ATATGATCCG CGGAGCAGAC CTGTCCCTCC GACGTCACCT GAGCTTCTCG 
GAGGACTCGG ACCTGTCCAG CGACGATGTC TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA 
GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC CCTGAATTTC ACATGCACAG AGATGAGTCG 
GTGTATGTCC CCCACGCCTT GGCATTCAGA CGGTCATACT CATCAAAGAA AACCTACACG 
GACGAGGAGC TAAGCGCTAA GCTAACAAGG CGGGTGCAGA AAGCGGCACG GAGACAGGCC 
AAGCAGGAGG AGCTAAAGCG GCTGCATCGG GCACAGATCA TTCAGCGCCA GCTGGAACAA 
GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG 
GGAGAAGCAG ACTATTGGGG AGAGTCTTAT TACAGTGACC TCATCGATTT GCACCTGGGT 
GTTGATCCCG TTGCCGGGAC TCCGCGCCGT AGACCTCTCT CCTTCTGCCC CTGCTGTGCC 
CACGAAGGGA TGGGAAAGAA GGACGACCCG AAACTCATGC AGGAATGGTT CCGCCTGGTG 
CAGGAGAAGA ACGCGCTGGT GCGATACGAA TCGGAGCTGA TGATATTTGC CCGCGAGTTG 
GAGTTGGAGG ACAGACAGAG CCGACTTCAG CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA 
GATCAATTGA AGACGGAGCA GGAGCTGACG GACGAGAAGA AGATCCTGAA CGAGATGCTG 
GACGTGGTGG AACAGAGAGA TGCTCTGGTG GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG 
AAAGAGGAGG ACAAAGATCT GGAGGAGGTC ATGTTATCCA AGGGGTTTAA CCTCAACTGG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898577.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754437.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X6, mRNA.

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CloneID	OXa54209
Clone ID Related Accession (Same CDS sequence)	XM_031898577.1
Accession Version	XM_031898577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGTTGATCC CGTTGCCGGG ACTCCGCGCC GTAGACCTCT CTCCTTCTGC 
CCCTGCTGTG CCCACGAAGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754437.1
CDS	358..7035
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898577.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898577.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGTTGATCC CGTTGCCGGG ACTCCGCGCC GTAGACCTCT CTCCTTCTGC 
CCCTGCTGTG CCCACGAAGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959365.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814819.2 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X7, mRNA.

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CloneID	OXa54210
Clone ID Related Accession (Same CDS sequence)	XM_012959365.3
Accession Version	XM_012959365.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGA CTATTGGGGA GAGTCTTATT ACAGTGACCT CATCGATTTG 
CACCTGGGTG TTGATCCCGT TGCCGGGACT CCGCGCCGTA GACCTCTCTC CTTCTGCCCC 
TGCTGTGCCC ACGAAGGGAT GGGAAAGAAG GACGACCCGA AACTCATGCA GGAATGGTTC 
CGCCTGGTGC AGGAGAAGAA CGCGCTGGTG CGATACGAAT CGGAGCTGAT GATATTTGCC 
CGCGAGTTGG AGTTGGAGGA CAGACAGAGC CGACTTCAGC AGGAGCTGCG GGAGCGAATG 
GCAGTGGAAG ATCAATTGAA GACGGAGCAG GAGCTGACGG ACGAGAAGAA GATCCTGAAC 
GAGATGCTGG ACGTGGTGGA ACAGAGAGAT GCTCTGGTGG CCCTGTTGGA GGAGCAGAGA 
CTGCGGGAGA AAGAGGAGGA CAAAGATCTG GAGGAGGTCA TGTTATCCAA GGGGTTTAAC 
CTCAACTGGT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814819.2
CDS	358..7032
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959365.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959365.3

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGA CTATTGGGGA GAGTCTTATT ACAGTGACCT CATCGATTTG 
CACCTGGGTG TTGATCCCGT TGCCGGGACT CCGCGCCGTA GACCTCTCTC CTTCTGCCCC 
TGCTGTGCCC ACGAAGGGAT GGGAAAGAAG GACGACCCGA AACTCATGCA GGAATGGTTC 
CGCCTGGTGC AGGAGAAGAA CGCGCTGGTG CGATACGAAT CGGAGCTGAT GATATTTGCC 
CGCGAGTTGG AGTTGGAGGA CAGACAGAGC CGACTTCAGC AGGAGCTGCG GGAGCGAATG 
GCAGTGGAAG ATCAATTGAA GACGGAGCAG GAGCTGACGG ACGAGAAGAA GATCCTGAAC 
GAGATGCTGG ACGTGGTGGA ACAGAGAGAT GCTCTGGTGG CCCTGTTGGA GGAGCAGAGA 
CTGCGGGAGA AAGAGGAGGA CAAAGATCTG GAGGAGGTCA TGTTATCCAA GGGGTTTAAC 
CTCAACTGGT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898578.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754438.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X8, mRNA.

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CloneID	OXa54211
Clone ID Related Accession (Same CDS sequence)	XM_031898578.1
Accession Version	XM_031898578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCTACT 
GGCGAGTATG AAGAAGGTGA GGAGGAGGAG CTGGGTACTT GCCTGGCGCA GGCTGAAGCA 
GCTGGTGATG GGGACACCTC CGCAGACCTT GGTGATGATG ATGACATCCC GTCCGACGCC 
GAGGCCGAAG AACGGCTCCA GCAGTCTCAG AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA 
GAGCCTAAGG TGGAGCAACC CGTCCTGCCA GCAATTGCAG ATTCTGAGAG CGTCCCCAGC 
CCAGTCTCTC TGGCACCGAA AGCCCCCGTG CTGTCTCCAA GAAGGACACT CAAAGCGGAA 
GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG GCAGTGACCT CTCCTGGGGG TCGGTTCTTT 
GCGGATCCGT ATCTGCCATT GGACAGCAGC AACAAAGCCT TAGTGGAAGC AAAAACTCCA 
ACTCCTCTAT CTCCTGTTTG CTCCCAACCA GTCCCATCGC AAGAACCTGT TATCCCTACA 
TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA AACACGCGCC CACTTATATC CCCATCAGCT 
CCTATTTGTT CCCAGCCACT GCCGTCCTCA GAGGGTACCC TAAATGCTAC AGACAGGCCC 
CCCGTGCGTC AGCAGCCATC CCCTCTAATC CCCTCTACTT CTTTAACCAA ACTGCCTTTT 
ACTGGTCACG TCATGGGATC TGAACAATTA GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA 
CGTATTGACC TTATCGAAGA GTTCTGGACA AAAAGCGCCG AGATCAGGCG CAGCTTAGGG 
CTGGCCCCCG TGGACAGAGA TCGGGTACAT GAGATCAGTT TCCCTGCCCT AGCTTCAACT 
CCTCTTCAGC CTCAGGCACC TAAATCCAAA CCTGAGGAGC AAAAGTCACC GTCTCTGAAG 
CCACAACAAG TTGCAAGAAG AGTGAACCCT TCCCGGCTGG ACATTGAACT GCTCTCCCCT 
CTGACTCCAA TATCCCCTTC TGAGAAGGAG CTGAAGAGCT CAGATGGTGA GAAGAAGGAT 
ATCTCCACCA GCTCTGGCTT AGGCCTGAAT GGCAGTTCCA TAACACGTAC TGCCGCCAGT 
GAAAGCTTTA ACACCTCTGA TTCAGCCATG CTTACTCCAC CATCCAGCCC TCCACCACCT 
CCTCCCCAAG GGGAAGAGCC AGCTACTCTA CGTAGAAGCC GTACTCCTTG CCAGAATGGT 
CCAGAATCTA TCCCAGCCCC TGCCCTAGCA CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC 
CCCCGCTGGG AAGAATCTGT ACGTAAGTCA TTTGTAGAAA GTGTAGACGA GATCCCCTTT 
GCCGATGATG TGGAGGACAC GTACGACGAA AGAACGGAGA ATTCCAGCCT TCAAGAGAGG 
TTCTTTACCC CACCAACGAG TCGGGTTAAA CCACATAGAA TTGGTTTCTC CAAGGAAAAT 
GGAGAGATCC CATCTTCTTC GGAGAGAGTG CACCACAGAA AAAGGCTCTT GCCCGACGTC 
ACTCAGGAGG CCAAAGAACG GGCAGAGGAG CGGATGAAAG CCCGGGAGAA GTCTGTGAAG 
AGCCAGGCAC TGAAAGACGC CATAGCACAA CAGCGGCACA AAATGAAACA ACTAGAACCA 
GGGGCAAGAT CGGCCAAAGC TCTGCCATCT ACCGTTATGT TCAGCGATTA CCCTGCAGTA 
TCTCCTCTCC TGAGAACCCA GACCGAGACT CCCACCCTGA AGCTGGATAC CGACCAGGAG 
GTCTTTATAA CCCCCCCAGC AGCTCCTGAC GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC 
GGGAAACCCA AGAAAAGGCC GTCTATATTC TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG 
GGGGAAAGCA GTCGGGCGTC TGATAAACAG GCGACGGACG AGAGCACCAA ACCCAAGTCC 
TTGTGGAAAT CCGTCTTCTC CGGGTATAAG AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG 
GAGGACAAGT CTACTCCGAG CACTCCATCC AGCAGCACCA CCGTGGACTC TGGGAAAGCC 
AAGGTGGCAA ATATGATCCG CGGAGCAGAC CTGTCCCTCC GACGTCACCT GAGCTTCTCG 
GAGGACTCGG ACCTGTCCAG CGACGATGTC TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA 
GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC CCTGAATTTC ACATGCACAG AGATGAGTCG 
GTGTATGTCC CCCACGCCTT GGCATTCAGA CGGTCATACT CATCAAAGAA AACCTACACG 
GACGAGGAGC TAAGCGCTAA GCTAACAAGG CGGGTGCAGA AAGCGGCACG GAGACAGGCC 
AAGCAGGAGG AGCTAAAGCG GCTGCATCGG GCACAGATCA TTCAGCGCCA GCTGGAACAA 
GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG 
GGAGAAGCAG ACTATTGGGG AGAGTCTTAT TACAGTGACC TCATCGATTT GCACCTGGGT 
GTTGATCCCG TTGCCGGGAC TCCGCGCCGT AGACCTCTCT CCTTCTGCCC CTGCTGTGCC 
CACGAAGGGA TGGGAAAGAA GGACGACCCG AAACTCATGC AGGAATGGTT CCGCCTGGTG 
CAGGAGAAGA ACGCGCTGGT GCGATACGAA TCGGAGCTGA TGATATTTGC CCGCGAGTTG 
GAGTTGGAGG ACAGACAGAG CCGACTTCAG CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA 
GATCAATTGA AGACGGAGCA GGAGCTGACG GACGAGAAGA AGATCCTGAA CGAGATGCTG 
GACGTGGTGG AACAGAGAGA TGCTCTGGTG GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG 
AAAGAGGAGG ACAAAGATCT GGAGGAGGTC ATGTTATCCA AGGGGTTTAA CCTCAACTGG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754438.1
CDS	358..7023
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898578.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898578.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCTACT 
GGCGAGTATG AAGAAGGTGA GGAGGAGGAG CTGGGTACTT GCCTGGCGCA GGCTGAAGCA 
GCTGGTGATG GGGACACCTC CGCAGACCTT GGTGATGATG ATGACATCCC GTCCGACGCC 
GAGGCCGAAG AACGGCTCCA GCAGTCTCAG AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA 
GAGCCTAAGG TGGAGCAACC CGTCCTGCCA GCAATTGCAG ATTCTGAGAG CGTCCCCAGC 
CCAGTCTCTC TGGCACCGAA AGCCCCCGTG CTGTCTCCAA GAAGGACACT CAAAGCGGAA 
GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG GCAGTGACCT CTCCTGGGGG TCGGTTCTTT 
GCGGATCCGT ATCTGCCATT GGACAGCAGC AACAAAGCCT TAGTGGAAGC AAAAACTCCA 
ACTCCTCTAT CTCCTGTTTG CTCCCAACCA GTCCCATCGC AAGAACCTGT TATCCCTACA 
TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA AACACGCGCC CACTTATATC CCCATCAGCT 
CCTATTTGTT CCCAGCCACT GCCGTCCTCA GAGGGTACCC TAAATGCTAC AGACAGGCCC 
CCCGTGCGTC AGCAGCCATC CCCTCTAATC CCCTCTACTT CTTTAACCAA ACTGCCTTTT 
ACTGGTCACG TCATGGGATC TGAACAATTA GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA 
CGTATTGACC TTATCGAAGA GTTCTGGACA AAAAGCGCCG AGATCAGGCG CAGCTTAGGG 
CTGGCCCCCG TGGACAGAGA TCGGGTACAT GAGATCAGTT TCCCTGCCCT AGCTTCAACT 
CCTCTTCAGC CTCAGGCACC TAAATCCAAA CCTGAGGAGC AAAAGTCACC GTCTCTGAAG 
CCACAACAAG TTGCAAGAAG AGTGAACCCT TCCCGGCTGG ACATTGAACT GCTCTCCCCT 
CTGACTCCAA TATCCCCTTC TGAGAAGGAG CTGAAGAGCT CAGATGGTGA GAAGAAGGAT 
ATCTCCACCA GCTCTGGCTT AGGCCTGAAT GGCAGTTCCA TAACACGTAC TGCCGCCAGT 
GAAAGCTTTA ACACCTCTGA TTCAGCCATG CTTACTCCAC CATCCAGCCC TCCACCACCT 
CCTCCCCAAG GGGAAGAGCC AGCTACTCTA CGTAGAAGCC GTACTCCTTG CCAGAATGGT 
CCAGAATCTA TCCCAGCCCC TGCCCTAGCA CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC 
CCCCGCTGGG AAGAATCTGT ACGTAAGTCA TTTGTAGAAA GTGTAGACGA GATCCCCTTT 
GCCGATGATG TGGAGGACAC GTACGACGAA AGAACGGAGA ATTCCAGCCT TCAAGAGAGG 
TTCTTTACCC CACCAACGAG TCGGGTTAAA CCACATAGAA TTGGTTTCTC CAAGGAAAAT 
GGAGAGATCC CATCTTCTTC GGAGAGAGTG CACCACAGAA AAAGGCTCTT GCCCGACGTC 
ACTCAGGAGG CCAAAGAACG GGCAGAGGAG CGGATGAAAG CCCGGGAGAA GTCTGTGAAG 
AGCCAGGCAC TGAAAGACGC CATAGCACAA CAGCGGCACA AAATGAAACA ACTAGAACCA 
GGGGCAAGAT CGGCCAAAGC TCTGCCATCT ACCGTTATGT TCAGCGATTA CCCTGCAGTA 
TCTCCTCTCC TGAGAACCCA GACCGAGACT CCCACCCTGA AGCTGGATAC CGACCAGGAG 
GTCTTTATAA CCCCCCCAGC AGCTCCTGAC GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC 
GGGAAACCCA AGAAAAGGCC GTCTATATTC TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG 
GGGGAAAGCA GTCGGGCGTC TGATAAACAG GCGACGGACG AGAGCACCAA ACCCAAGTCC 
TTGTGGAAAT CCGTCTTCTC CGGGTATAAG AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG 
GAGGACAAGT CTACTCCGAG CACTCCATCC AGCAGCACCA CCGTGGACTC TGGGAAAGCC 
AAGGTGGCAA ATATGATCCG CGGAGCAGAC CTGTCCCTCC GACGTCACCT GAGCTTCTCG 
GAGGACTCGG ACCTGTCCAG CGACGATGTC TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA 
GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC CCTGAATTTC ACATGCACAG AGATGAGTCG 
GTGTATGTCC CCCACGCCTT GGCATTCAGA CGGTCATACT CATCAAAGAA AACCTACACG 
GACGAGGAGC TAAGCGCTAA GCTAACAAGG CGGGTGCAGA AAGCGGCACG GAGACAGGCC 
AAGCAGGAGG AGCTAAAGCG GCTGCATCGG GCACAGATCA TTCAGCGCCA GCTGGAACAA 
GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG 
GGAGAAGCAG ACTATTGGGG AGAGTCTTAT TACAGTGACC TCATCGATTT GCACCTGGGT 
GTTGATCCCG TTGCCGGGAC TCCGCGCCGT AGACCTCTCT CCTTCTGCCC CTGCTGTGCC 
CACGAAGGGA TGGGAAAGAA GGACGACCCG AAACTCATGC AGGAATGGTT CCGCCTGGTG 
CAGGAGAAGA ACGCGCTGGT GCGATACGAA TCGGAGCTGA TGATATTTGC CCGCGAGTTG 
GAGTTGGAGG ACAGACAGAG CCGACTTCAG CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA 
GATCAATTGA AGACGGAGCA GGAGCTGACG GACGAGAAGA AGATCCTGAA CGAGATGCTG 
GACGTGGTGG AACAGAGAGA TGCTCTGGTG GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG 
AAAGAGGAGG ACAAAGATCT GGAGGAGGTC ATGTTATCCA AGGGGTTTAA CCTCAACTGG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898579.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754439.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X9, mRNA.

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CloneID	OXa54212
Clone ID Related Accession (Same CDS sequence)	XM_031898579.1
Accession Version	XM_031898579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGGGATGG GAAAGAAGGA CGACCCGAAA CTCATGCAGG AATGGTTCCG CCTGGTGCAG 
GAGAAGAACG CGCTGGTGCG ATACGAATCG GAGCTGATGA TATTTGCCCG CGAGTTGGAG 
TTGGAGGACA GACAGAGCCG ACTTCAGCAG GAGCTGCGGG AGCGAATGGC AGTGGAAGAT 
CAATTGAAGA CGGAGCAGGA GCTGACGGAC GAGAAGAAGA TCCTGAACGA GATGCTGGAC 
GTGGTGGAAC AGAGAGATGC TCTGGTGGCC CTGTTGGAGG AGCAGAGACT GCGGGAGAAA 
GAGGAGGACA AAGATCTGGA GGAGGTCATG TTATCCAAGG GGTTTAACCT CAACTGGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754439.1
CDS	358..7020
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898579.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898579.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGGGATGG GAAAGAAGGA CGACCCGAAA CTCATGCAGG AATGGTTCCG CCTGGTGCAG 
GAGAAGAACG CGCTGGTGCG ATACGAATCG GAGCTGATGA TATTTGCCCG CGAGTTGGAG 
TTGGAGGACA GACAGAGCCG ACTTCAGCAG GAGCTGCGGG AGCGAATGGC AGTGGAAGAT 
CAATTGAAGA CGGAGCAGGA GCTGACGGAC GAGAAGAAGA TCCTGAACGA GATGCTGGAC 
GTGGTGGAAC AGAGAGATGC TCTGGTGGCC CTGTTGGAGG AGCAGAGACT GCGGGAGAAA 
GAGGAGGACA AAGATCTGGA GGAGGTCATG TTATCCAAGG GGTTTAACCT CAACTGGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898580.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754440.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X10, mRNA.

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CloneID	OXa54213
Clone ID Related Accession (Same CDS sequence)	XM_031898580.1
Accession Version	XM_031898580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG ACAACCCCGG TGCTCGGCAG TAGCCGTTGG 
GAGCAGTGTC CCACACTGGA GAGGTCCGAG ACCCAATCCC GCTCTCCGGC TGGCCAATGG 
CAGCAGACCC TTGCTCAGCG TTCCTCCGAC CGCCCCTCGT CCCCCTCGTC CGATGCCCTT 
CGACAGAAGT ATATAAAGAT GTACACAGGT GGAGTGAGCT CATTGGCTGA GCAAATAGCC 
AATCAGCTTC AAAAGAAGGA AACGCCAAAA CCTCTGTTCG GGAAGAAAGT GGGCTCATTT 
AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG AGCGACGTCT GCTATTTCTG CCGCCGGAGG 
GTTTACGTCA TGGAGCGGCT CAGCGCCGAG GGCAAGTTCT TCCACCGGAG CTGCTTTAAG 
TGTGACTACT GTAGCACAAC GCTGCGCCTC TCATGTTATG CCTATGACCT TGAGGATGGA 
AAATTTTACT GCAAACCTCA CTACTGTTAC CGCCGGTCCG GATACAGCCA GAGGAAGAGG 
CCGGCCATGC CTGCACTTTC GGGAAAAGAA GTCACAAGGA CTGTGGAAGA CACCCCCGAC 
GCGGAAGACC AAGGACGAGC CCCGCTGCTG CCCGCCCCCG CTGCTGAGAA GACCCCAGGC 
CCCAGTGTGA ACGGCGTGGA AGATCACAGC GCCGCCAAGA GGCTGAGAGC CACCCCAGAG 
CGCATTGAGC TTGAGAATTA CCGCGTCTCC CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC 
GAGGAAACCC TGGCGGAGCA TAATCTGAGC AGCCTATTGG ATAAAGGGGA GGTGGAAGAG 
GTGGCCAGCA GCAGTTCCGA GTCCGAGATG GAGGAGGAGG AGGACGAAGA AGAAGAGGAG 
GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA GATCTGGGTG GGGTGCCGTG GAAAGAGGCC 
GTCAAGATCC ACGCCAAGTT AAAGGGTCAG GACATAGATG TGGAGGTTGT GGGAACCCAG 
GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG GAGGAGGAAG AAGAGGACGA AGAGGAGGAA 
GATGAAGAGG TATCAAGTGA AGAGGGTGAG TACTGCCCAT GGGAGAGAGA GCTTCAGCAG 
GGCCTGTGGA TACAGCGTAT CTCTGATGAA GATACAGTTG GTATATTTAA AGCCGGGAAC 
CTCAGACTCC AGCAAGCCCT CTCCCCTGTT GACCCCACGG AAATAAAGGC AGACGTGCGC 
TGGACACACG TCCGCGAGGT TAAGGAAGAG CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA 
GTGAGCATGT CCTCTCCTTC CAAAGCGCGT CATCTCCATT CCCACCGTCA CGACCCAGTG 
AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG 
GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA GCAGCTGGTG ATGGGGACAC CTCCGCAGAC 
CTTGGTGATG ATGATGACAT CCCGTCCGAC GCCGAGGCCG AAGAACGGCT CCAGCAGTCT 
CAGAGGTTTA AATTGAAGCT GGATGTAGCC CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG 
CCAGCAATTG CAGATTCTGA GAGCGTCCCC AGCCCAGTCT CTCTGGCACC GAAAGCCCCC 
GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG GAAGCAGAAA AGGTTCCTGT GATGTCTCCG 
AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC TTTGCGGATC CGTATCTGCC ATTGGACAGC 
AGCAACAAAG CCTTAGTGGA AGCAAAAACT CCAACTCCTC TATCTCCTGT TTGCTCCCAA 
CCAGTCCCAT CGCAAGAACC TGTTATCCCT ACATCTCCTG TGAAGTCTCC CTCAGTCCCA 
GCAAACACGC GCCCACTTAT ATCCCCATCA GCTCCTATTT GTTCCCAGCC ACTGCCGTCC 
TCAGAGGGTA CCCTAAATGC TACAGACAGG CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA 
ATCCCCTCTA CTTCTTTAAC CAAACTGCCT TTTACTGGTC ACGTCATGGG ATCTGAACAA 
TTAGTTAGCC CTAATGCAAA AGAGGTCCTG AAACGTATTG ACCTTATCGA AGAGTTCTGG 
ACAAAAAGCG CCGAGATCAG GCGCAGCTTA GGGCTGGCCC CCGTGGACAG AGATCGGGTA 
CATGAGATCA GTTTCCCTGC CCTAGCTTCA ACTCCTCTTC AGCCTCAGGC ACCTAAATCC 
AAACCTGAGG AGCAAAAGTC ACCGTCTCTG AAGCCACAAC AAGTTGCAAG AAGAGTGAAC 
CCTTCCCGGC TGGACATTGA ACTGCTCTCC CCTCTGACTC CAATATCCCC TTCTGAGAAG 
GAGCTGAAGA GCTCAGATGG TGAGAAGAAG GATATCTCCA CCAGCTCTGG CTTAGGCCTG 
AATGGCAGTT CCATAACACG TACTGCCGCC AGTGAAAGCT TTAACACCTC TGATTCAGCC 
ATGCTTACTC CACCATCCAG CCCTCCACCA CCTCCTCCCC AAGGGGAAGA GCCAGCTACT 
CTACGTAGAA GCCGTACTCC TTGCCAGAAT GGTCCAGAAT CTATCCCAGC CCCTGCCCTA 
GCACAGAGCA AGCCCCCTGA GCCCAAAAAG TCCCCCCGCT GGGAAGAATC TGTACGTAAG 
TCATTTGTAG AAAGTGTAGA CGAGATCCCC TTTGCCGATG ATGTGGAGGA CACGTACGAC 
GAAAGAACGG AGAATTCCAG CCTTCAAGAG AGGTTCTTTA CCCCACCAAC GAGTCGGGTT 
AAACCACATA GAATTGGTTT CTCCAAGGAA AATGGAGAGA TCCCATCTTC TTCGGAGAGA 
GTGCACCACA GAAAAAGGCT CTTGCCCGAC GTCACTCAGG AGGCCAAAGA ACGGGCAGAG 
GAGCGGATGA AAGCCCGGGA GAAGTCTGTG AAGAGCCAGG CACTGAAAGA CGCCATAGCA 
CAACAGCGGC ACAAAATGAA ACAACTAGAA CCAGGGGCAA GATCGGCCAA AGCTCTGCCA 
TCTACCGTTA TGTTCAGCGA TTACCCTGCA GTATCTCCTC TCCTGAGAAC CCAGACCGAG 
ACTCCCACCC TGAAGCTGGA TACCGACCAG GAGGTCTTTA TAACCCCCCC AGCAGCTCCT 
GACGGCTCCG TCACTTCCTC GGAAGGTTCA GGCGGGAAAC CCAAGAAAAG GCCGTCTATA 
TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC AAGGGGGAAA GCAGTCGGGC GTCTGATAAA 
CAGGCGACGG ACGAGAGCAC CAAACCCAAG TCCTTGTGGA AATCCGTCTT CTCCGGGTAT 
AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG GCGGAGGACA AGTCTACTCC GAGCACTCCA 
TCCAGCAGCA CCACCGTGGA CTCTGGGAAA GCCAAGGTGG CAAATATGAT CCGCGGAGCA 
GACCTGTCCC TCCGACGTCA CCTGAGCTTC TCGGAGGACT CGGACCTGTC CAGCGACGAT 
GTCTTGGAAA AATCCTCCCA GAAATCTAAG CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG 
GCCCCTGAAT TTCACATGCA CAGAGATGAG TCGGTGTATG TCCCCCACGC CTTGGCATTC 
AGACGGTCAT ACTCATCAAA GAAAACCTAC ACGGACGAGG AGCTAAGCGC TAAGCTAACA 
AGGCGGGTGC AGAAAGCGGC ACGGAGACAG GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT 
CGGGCACAGA TCATTCAGCG CCAGCTGGAA CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG 
GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC CGGGGAGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA TTTGCACCTG GGTGTTGATC CCGTTGCCGG GACTCCGCGC 
CGTAGACCTC TCTCCTTCTG CCCCTGCTGT GCCCACGAAG GGATGGGAAA GAAGGACGAC 
CCGAAACTCA TGCAGGAATG GTTCCGCCTG GTGCAGGAGA AGAACGCGCT GGTGCGATAC 
GAATCGGAGC TGATGATATT TGCCCGCGAG TTGGAGTTGG AGGACAGACA GAGCCGACTT 
CAGCAGGAGC TGCGGGAGCG AATGGCAGTG GAAGATCAAT TGAAGACGGA GCAGGAGCTG 
ACGGACGAGA AGAAGATCCT GAACGAGATG CTGGACGTGG TGGAACAGAG AGATGCTCTG 
GTGGCCCTGT TGGAGGAGCA GAGACTGCGG GAGAAAGAGG AGGACAAAGA TCTGGAGGAG 
GTCATGTTAT CCAAGGGGTT TAACCTCAAC TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754440.1
CDS	358..6996
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898580.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898580.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG ACAACCCCGG TGCTCGGCAG TAGCCGTTGG 
GAGCAGTGTC CCACACTGGA GAGGTCCGAG ACCCAATCCC GCTCTCCGGC TGGCCAATGG 
CAGCAGACCC TTGCTCAGCG TTCCTCCGAC CGCCCCTCGT CCCCCTCGTC CGATGCCCTT 
CGACAGAAGT ATATAAAGAT GTACACAGGT GGAGTGAGCT CATTGGCTGA GCAAATAGCC 
AATCAGCTTC AAAAGAAGGA AACGCCAAAA CCTCTGTTCG GGAAGAAAGT GGGCTCATTT 
AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG AGCGACGTCT GCTATTTCTG CCGCCGGAGG 
GTTTACGTCA TGGAGCGGCT CAGCGCCGAG GGCAAGTTCT TCCACCGGAG CTGCTTTAAG 
TGTGACTACT GTAGCACAAC GCTGCGCCTC TCATGTTATG CCTATGACCT TGAGGATGGA 
AAATTTTACT GCAAACCTCA CTACTGTTAC CGCCGGTCCG GATACAGCCA GAGGAAGAGG 
CCGGCCATGC CTGCACTTTC GGGAAAAGAA GTCACAAGGA CTGTGGAAGA CACCCCCGAC 
GCGGAAGACC AAGGACGAGC CCCGCTGCTG CCCGCCCCCG CTGCTGAGAA GACCCCAGGC 
CCCAGTGTGA ACGGCGTGGA AGATCACAGC GCCGCCAAGA GGCTGAGAGC CACCCCAGAG 
CGCATTGAGC TTGAGAATTA CCGCGTCTCC CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC 
GAGGAAACCC TGGCGGAGCA TAATCTGAGC AGCCTATTGG ATAAAGGGGA GGTGGAAGAG 
GTGGCCAGCA GCAGTTCCGA GTCCGAGATG GAGGAGGAGG AGGACGAAGA AGAAGAGGAG 
GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA GATCTGGGTG GGGTGCCGTG GAAAGAGGCC 
GTCAAGATCC ACGCCAAGTT AAAGGGTCAG GACATAGATG TGGAGGTTGT GGGAACCCAG 
GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG GAGGAGGAAG AAGAGGACGA AGAGGAGGAA 
GATGAAGAGG TATCAAGTGA AGAGGGTGAG TACTGCCCAT GGGAGAGAGA GCTTCAGCAG 
GGCCTGTGGA TACAGCGTAT CTCTGATGAA GATACAGTTG GTATATTTAA AGCCGGGAAC 
CTCAGACTCC AGCAAGCCCT CTCCCCTGTT GACCCCACGG AAATAAAGGC AGACGTGCGC 
TGGACACACG TCCGCGAGGT TAAGGAAGAG CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA 
GTGAGCATGT CCTCTCCTTC CAAAGCGCGT CATCTCCATT CCCACCGTCA CGACCCAGTG 
AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG 
GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA GCAGCTGGTG ATGGGGACAC CTCCGCAGAC 
CTTGGTGATG ATGATGACAT CCCGTCCGAC GCCGAGGCCG AAGAACGGCT CCAGCAGTCT 
CAGAGGTTTA AATTGAAGCT GGATGTAGCC CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG 
CCAGCAATTG CAGATTCTGA GAGCGTCCCC AGCCCAGTCT CTCTGGCACC GAAAGCCCCC 
GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG GAAGCAGAAA AGGTTCCTGT GATGTCTCCG 
AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC TTTGCGGATC CGTATCTGCC ATTGGACAGC 
AGCAACAAAG CCTTAGTGGA AGCAAAAACT CCAACTCCTC TATCTCCTGT TTGCTCCCAA 
CCAGTCCCAT CGCAAGAACC TGTTATCCCT ACATCTCCTG TGAAGTCTCC CTCAGTCCCA 
GCAAACACGC GCCCACTTAT ATCCCCATCA GCTCCTATTT GTTCCCAGCC ACTGCCGTCC 
TCAGAGGGTA CCCTAAATGC TACAGACAGG CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA 
ATCCCCTCTA CTTCTTTAAC CAAACTGCCT TTTACTGGTC ACGTCATGGG ATCTGAACAA 
TTAGTTAGCC CTAATGCAAA AGAGGTCCTG AAACGTATTG ACCTTATCGA AGAGTTCTGG 
ACAAAAAGCG CCGAGATCAG GCGCAGCTTA GGGCTGGCCC CCGTGGACAG AGATCGGGTA 
CATGAGATCA GTTTCCCTGC CCTAGCTTCA ACTCCTCTTC AGCCTCAGGC ACCTAAATCC 
AAACCTGAGG AGCAAAAGTC ACCGTCTCTG AAGCCACAAC AAGTTGCAAG AAGAGTGAAC 
CCTTCCCGGC TGGACATTGA ACTGCTCTCC CCTCTGACTC CAATATCCCC TTCTGAGAAG 
GAGCTGAAGA GCTCAGATGG TGAGAAGAAG GATATCTCCA CCAGCTCTGG CTTAGGCCTG 
AATGGCAGTT CCATAACACG TACTGCCGCC AGTGAAAGCT TTAACACCTC TGATTCAGCC 
ATGCTTACTC CACCATCCAG CCCTCCACCA CCTCCTCCCC AAGGGGAAGA GCCAGCTACT 
CTACGTAGAA GCCGTACTCC TTGCCAGAAT GGTCCAGAAT CTATCCCAGC CCCTGCCCTA 
GCACAGAGCA AGCCCCCTGA GCCCAAAAAG TCCCCCCGCT GGGAAGAATC TGTACGTAAG 
TCATTTGTAG AAAGTGTAGA CGAGATCCCC TTTGCCGATG ATGTGGAGGA CACGTACGAC 
GAAAGAACGG AGAATTCCAG CCTTCAAGAG AGGTTCTTTA CCCCACCAAC GAGTCGGGTT 
AAACCACATA GAATTGGTTT CTCCAAGGAA AATGGAGAGA TCCCATCTTC TTCGGAGAGA 
GTGCACCACA GAAAAAGGCT CTTGCCCGAC GTCACTCAGG AGGCCAAAGA ACGGGCAGAG 
GAGCGGATGA AAGCCCGGGA GAAGTCTGTG AAGAGCCAGG CACTGAAAGA CGCCATAGCA 
CAACAGCGGC ACAAAATGAA ACAACTAGAA CCAGGGGCAA GATCGGCCAA AGCTCTGCCA 
TCTACCGTTA TGTTCAGCGA TTACCCTGCA GTATCTCCTC TCCTGAGAAC CCAGACCGAG 
ACTCCCACCC TGAAGCTGGA TACCGACCAG GAGGTCTTTA TAACCCCCCC AGCAGCTCCT 
GACGGCTCCG TCACTTCCTC GGAAGGTTCA GGCGGGAAAC CCAAGAAAAG GCCGTCTATA 
TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC AAGGGGGAAA GCAGTCGGGC GTCTGATAAA 
CAGGCGACGG ACGAGAGCAC CAAACCCAAG TCCTTGTGGA AATCCGTCTT CTCCGGGTAT 
AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG GCGGAGGACA AGTCTACTCC GAGCACTCCA 
TCCAGCAGCA CCACCGTGGA CTCTGGGAAA GCCAAGGTGG CAAATATGAT CCGCGGAGCA 
GACCTGTCCC TCCGACGTCA CCTGAGCTTC TCGGAGGACT CGGACCTGTC CAGCGACGAT 
GTCTTGGAAA AATCCTCCCA GAAATCTAAG CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG 
GCCCCTGAAT TTCACATGCA CAGAGATGAG TCGGTGTATG TCCCCCACGC CTTGGCATTC 
AGACGGTCAT ACTCATCAAA GAAAACCTAC ACGGACGAGG AGCTAAGCGC TAAGCTAACA 
AGGCGGGTGC AGAAAGCGGC ACGGAGACAG GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT 
CGGGCACAGA TCATTCAGCG CCAGCTGGAA CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG 
GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC CGGGGAGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA TTTGCACCTG GGTGTTGATC CCGTTGCCGG GACTCCGCGC 
CGTAGACCTC TCTCCTTCTG CCCCTGCTGT GCCCACGAAG GGATGGGAAA GAAGGACGAC 
CCGAAACTCA TGCAGGAATG GTTCCGCCTG GTGCAGGAGA AGAACGCGCT GGTGCGATAC 
GAATCGGAGC TGATGATATT TGCCCGCGAG TTGGAGTTGG AGGACAGACA GAGCCGACTT 
CAGCAGGAGC TGCGGGAGCG AATGGCAGTG GAAGATCAAT TGAAGACGGA GCAGGAGCTG 
ACGGACGAGA AGAAGATCCT GAACGAGATG CTGGACGTGG TGGAACAGAG AGATGCTCTG 
GTGGCCCTGT TGGAGGAGCA GAGACTGCGG GAGAAAGAGG AGGACAAAGA TCTGGAGGAG 
GTCATGTTAT CCAAGGGGTT TAACCTCAAC TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898581.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754441.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X11, mRNA.

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CloneID	OXa54214
Clone ID Related Accession (Same CDS sequence)	XM_031898581.1
Accession Version	XM_031898581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGCCGGGAAC 
CTCAGACTCC AGCAAGCCCT CTCCCCTGTT GACCCCACGG AAATAAAGGC AGACGTGCGC 
TGGACACACG TCCGCGAGGT TAAGGAAGAG CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA 
GTGAGCATGT CCTCTCCTTC CAAAGCGCGT CATCTCCATT CCCACCGTCA CGACCCAGTG 
AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG 
GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA GCAGCTGGTG ATGGGGACAC CTCCGCAGAC 
CTTGGTGATG ATGATGACAT CCCGTCCGAC GCCGAGGCCG AAGAACGGCT CCAGCAGTCT 
CAGAGGTTTA AATTGAAGCT GGATGTAGCC CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG 
CCAGCAATTG CAGATTCTGA GAGCGTCCCC AGCCCAGTCT CTCTGGCACC GAAAGCCCCC 
GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG GAAGCAGAAA AGGTTCCTGT GATGTCTCCG 
AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC TTTGCGGATC CGTATCTGCC ATTGGACAGC 
AGCAACAAAG CCTTAGTGGA AGCAAAAACT CCAACTCCTC TATCTCCTGT TTGCTCCCAA 
CCAGTCCCAT CGCAAGAACC TGTTATCCCT ACATCTCCTG TGAAGTCTCC CTCAGTCCCA 
GCAAACACGC GCCCACTTAT ATCCCCATCA GCTCCTATTT GTTCCCAGCC ACTGCCGTCC 
TCAGAGGGTA CCCTAAATGC TACAGACAGG CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA 
ATCCCCTCTA CTTCTTTAAC CAAACTGCCT TTTACTGGTC ACGTCATGGG ATCTGAACAA 
TTAGTTAGCC CTAATGCAAA AGAGGTCCTG AAACGTATTG ACCTTATCGA AGAGTTCTGG 
ACAAAAAGCG CCGAGATCAG GCGCAGCTTA GGGCTGGCCC CCGTGGACAG AGATCGGGTA 
CATGAGATCA GTTTCCCTGC CCTAGCTTCA ACTCCTCTTC AGCCTCAGGC ACCTAAATCC 
AAACCTGAGG AGCAAAAGTC ACCGTCTCTG AAGCCACAAC AAGTTGCAAG AAGAGTGAAC 
CCTTCCCGGC TGGACATTGA ACTGCTCTCC CCTCTGACTC CAATATCCCC TTCTGAGAAG 
GAGCTGAAGA GCTCAGATGG TGAGAAGAAG GATATCTCCA CCAGCTCTGG CTTAGGCCTG 
AATGGCAGTT CCATAACACG TACTGCCGCC AGTGAAAGCT TTAACACCTC TGATTCAGCC 
ATGCTTACTC CACCATCCAG CCCTCCACCA CCTCCTCCCC AAGGGGAAGA GCCAGCTACT 
CTACGTAGAA GCCGTACTCC TTGCCAGAAT GGTCCAGAAT CTATCCCAGC CCCTGCCCTA 
GCACAGAGCA AGCCCCCTGA GCCCAAAAAG TCCCCCCGCT GGGAAGAATC TGTACGTAAG 
TCATTTGTAG AAAGTGTAGA CGAGATCCCC TTTGCCGATG ATGTGGAGGA CACGTACGAC 
GAAAGAACGG AGAATTCCAG CCTTCAAGAG AGGTTCTTTA CCCCACCAAC GAGTCGGGTT 
AAACCACATA GAATTGGTTT CTCCAAGGAA AATGGAGAGA TCCCATCTTC TTCGGAGAGA 
GTGCACCACA GAAAAAGGCT CTTGCCCGAC GTCACTCAGG AGGCCAAAGA ACGGGCAGAG 
GAGCGGATGA AAGCCCGGGA GAAGTCTGTG AAGAGCCAGG CACTGAAAGA CGCCATAGCA 
CAACAGCGGC ACAAAATGAA ACAACTAGAA CCAGGGGCAA GATCGGCCAA AGCTCTGCCA 
TCTACCGTTA TGTTCAGCGA TTACCCTGCA GTATCTCCTC TCCTGAGAAC CCAGACCGAG 
ACTCCCACCC TGAAGCTGGA TACCGACCAG GAGGTCTTTA TAACCCCCCC AGCAGCTCCT 
GACGGCTCCG TCACTTCCTC GGAAGGTTCA GGCGGGAAAC CCAAGAAAAG GCCGTCTATA 
TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC AAGGGGGAAA GCAGTCGGGC GTCTGATAAA 
CAGGCGACGG ACGAGAGCAC CAAACCCAAG TCCTTGTGGA AATCCGTCTT CTCCGGGTAT 
AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG GCGGAGGACA AGTCTACTCC GAGCACTCCA 
TCCAGCAGCA CCACCGTGGA CTCTGGGAAA GCCAAGGTGG CAAATATGAT CCGCGGAGCA 
GACCTGTCCC TCCGACGTCA CCTGAGCTTC TCGGAGGACT CGGACCTGTC CAGCGACGAT 
GTCTTGGAAA AATCCTCCCA GAAATCTAAG CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG 
GCCCCTGAAT TTCACATGCA CAGAGATGAG TCGGTGTATG TCCCCCACGC CTTGGCATTC 
AGACGGTCAT ACTCATCAAA GAAAACCTAC ACGGACGAGG AGCTAAGCGC TAAGCTAACA 
AGGCGGGTGC AGAAAGCGGC ACGGAGACAG GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT 
CGGGCACAGA TCATTCAGCG CCAGCTGGAA CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG 
GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC CGGGGAGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA TTTGCACCTG GGTGTTGATC CCGTTGCCGG GACTCCGCGC 
CGTAGACCTC TCTCCTTCTG CCCCTGCTGT GCCCACGAAG GGATGGGAAA GAAGGACGAC 
CCGAAACTCA TGCAGGAATG GTTCCGCCTG GTGCAGGAGA AGAACGCGCT GGTGCGATAC 
GAATCGGAGC TGATGATATT TGCCCGCGAG TTGGAGTTGG AGGACAGACA GAGCCGACTT 
CAGCAGGAGC TGCGGGAGCG AATGGCAGTG GAAGATCAAT TGAAGACGGA GCAGGAGCTG 
ACGGACGAGA AGAAGATCCT GAACGAGATG CTGGACGTGG TGGAACAGAG AGATGCTCTG 
GTGGCCCTGT TGGAGGAGCA GAGACTGCGG GAGAAAGAGG AGGACAAAGA TCTGGAGGAG 
GTCATGTTAT CCAAGGGGTT TAACCTCAAC TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754441.1
CDS	358..6996
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898581.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898581.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGCCGGGAAC 
CTCAGACTCC AGCAAGCCCT CTCCCCTGTT GACCCCACGG AAATAAAGGC AGACGTGCGC 
TGGACACACG TCCGCGAGGT TAAGGAAGAG CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA 
GTGAGCATGT CCTCTCCTTC CAAAGCGCGT CATCTCCATT CCCACCGTCA CGACCCAGTG 
AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG 
GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA GCAGCTGGTG ATGGGGACAC CTCCGCAGAC 
CTTGGTGATG ATGATGACAT CCCGTCCGAC GCCGAGGCCG AAGAACGGCT CCAGCAGTCT 
CAGAGGTTTA AATTGAAGCT GGATGTAGCC CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG 
CCAGCAATTG CAGATTCTGA GAGCGTCCCC AGCCCAGTCT CTCTGGCACC GAAAGCCCCC 
GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG GAAGCAGAAA AGGTTCCTGT GATGTCTCCG 
AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC TTTGCGGATC CGTATCTGCC ATTGGACAGC 
AGCAACAAAG CCTTAGTGGA AGCAAAAACT CCAACTCCTC TATCTCCTGT TTGCTCCCAA 
CCAGTCCCAT CGCAAGAACC TGTTATCCCT ACATCTCCTG TGAAGTCTCC CTCAGTCCCA 
GCAAACACGC GCCCACTTAT ATCCCCATCA GCTCCTATTT GTTCCCAGCC ACTGCCGTCC 
TCAGAGGGTA CCCTAAATGC TACAGACAGG CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA 
ATCCCCTCTA CTTCTTTAAC CAAACTGCCT TTTACTGGTC ACGTCATGGG ATCTGAACAA 
TTAGTTAGCC CTAATGCAAA AGAGGTCCTG AAACGTATTG ACCTTATCGA AGAGTTCTGG 
ACAAAAAGCG CCGAGATCAG GCGCAGCTTA GGGCTGGCCC CCGTGGACAG AGATCGGGTA 
CATGAGATCA GTTTCCCTGC CCTAGCTTCA ACTCCTCTTC AGCCTCAGGC ACCTAAATCC 
AAACCTGAGG AGCAAAAGTC ACCGTCTCTG AAGCCACAAC AAGTTGCAAG AAGAGTGAAC 
CCTTCCCGGC TGGACATTGA ACTGCTCTCC CCTCTGACTC CAATATCCCC TTCTGAGAAG 
GAGCTGAAGA GCTCAGATGG TGAGAAGAAG GATATCTCCA CCAGCTCTGG CTTAGGCCTG 
AATGGCAGTT CCATAACACG TACTGCCGCC AGTGAAAGCT TTAACACCTC TGATTCAGCC 
ATGCTTACTC CACCATCCAG CCCTCCACCA CCTCCTCCCC AAGGGGAAGA GCCAGCTACT 
CTACGTAGAA GCCGTACTCC TTGCCAGAAT GGTCCAGAAT CTATCCCAGC CCCTGCCCTA 
GCACAGAGCA AGCCCCCTGA GCCCAAAAAG TCCCCCCGCT GGGAAGAATC TGTACGTAAG 
TCATTTGTAG AAAGTGTAGA CGAGATCCCC TTTGCCGATG ATGTGGAGGA CACGTACGAC 
GAAAGAACGG AGAATTCCAG CCTTCAAGAG AGGTTCTTTA CCCCACCAAC GAGTCGGGTT 
AAACCACATA GAATTGGTTT CTCCAAGGAA AATGGAGAGA TCCCATCTTC TTCGGAGAGA 
GTGCACCACA GAAAAAGGCT CTTGCCCGAC GTCACTCAGG AGGCCAAAGA ACGGGCAGAG 
GAGCGGATGA AAGCCCGGGA GAAGTCTGTG AAGAGCCAGG CACTGAAAGA CGCCATAGCA 
CAACAGCGGC ACAAAATGAA ACAACTAGAA CCAGGGGCAA GATCGGCCAA AGCTCTGCCA 
TCTACCGTTA TGTTCAGCGA TTACCCTGCA GTATCTCCTC TCCTGAGAAC CCAGACCGAG 
ACTCCCACCC TGAAGCTGGA TACCGACCAG GAGGTCTTTA TAACCCCCCC AGCAGCTCCT 
GACGGCTCCG TCACTTCCTC GGAAGGTTCA GGCGGGAAAC CCAAGAAAAG GCCGTCTATA 
TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC AAGGGGGAAA GCAGTCGGGC GTCTGATAAA 
CAGGCGACGG ACGAGAGCAC CAAACCCAAG TCCTTGTGGA AATCCGTCTT CTCCGGGTAT 
AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG GCGGAGGACA AGTCTACTCC GAGCACTCCA 
TCCAGCAGCA CCACCGTGGA CTCTGGGAAA GCCAAGGTGG CAAATATGAT CCGCGGAGCA 
GACCTGTCCC TCCGACGTCA CCTGAGCTTC TCGGAGGACT CGGACCTGTC CAGCGACGAT 
GTCTTGGAAA AATCCTCCCA GAAATCTAAG CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG 
GCCCCTGAAT TTCACATGCA CAGAGATGAG TCGGTGTATG TCCCCCACGC CTTGGCATTC 
AGACGGTCAT ACTCATCAAA GAAAACCTAC ACGGACGAGG AGCTAAGCGC TAAGCTAACA 
AGGCGGGTGC AGAAAGCGGC ACGGAGACAG GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT 
CGGGCACAGA TCATTCAGCG CCAGCTGGAA CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG 
GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC CGGGGAGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA TTTGCACCTG GGTGTTGATC CCGTTGCCGG GACTCCGCGC 
CGTAGACCTC TCTCCTTCTG CCCCTGCTGT GCCCACGAAG GGATGGGAAA GAAGGACGAC 
CCGAAACTCA TGCAGGAATG GTTCCGCCTG GTGCAGGAGA AGAACGCGCT GGTGCGATAC 
GAATCGGAGC TGATGATATT TGCCCGCGAG TTGGAGTTGG AGGACAGACA GAGCCGACTT 
CAGCAGGAGC TGCGGGAGCG AATGGCAGTG GAAGATCAAT TGAAGACGGA GCAGGAGCTG 
ACGGACGAGA AGAAGATCCT GAACGAGATG CTGGACGTGG TGGAACAGAG AGATGCTCTG 
GTGGCCCTGT TGGAGGAGCA GAGACTGCGG GAGAAAGAGG AGGACAAAGA TCTGGAGGAG 
GTCATGTTAT CCAAGGGGTT TAACCTCAAC TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959367.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814821.2 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X12, mRNA.

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CloneID	OXa54215
Clone ID Related Accession (Same CDS sequence)	XM_012959367.3
Accession Version	XM_012959367.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959367.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGT TGATCCCGTT GCCGGGACTC CGCGCCGTAG ACCTCTCTCC 
TTCTGCCCCT GCTGTGCCCA CGAAGGGATG GGAAAGAAGG ACGACCCGAA ACTCATGCAG 
GAATGGTTCC GCCTGGTGCA GGAGAAGAAC GCGCTGGTGC GATACGAATC GGAGCTGATG 
ATATTTGCCC GCGAGTTGGA GTTGGAGGAC AGACAGAGCC GACTTCAGCA GGAGCTGCGG 
GAGCGAATGG CAGTGGAAGA TCAATTGAAG ACGGAGCAGG AGCTGACGGA CGAGAAGAAG 
ATCCTGAACG AGATGCTGGA CGTGGTGGAA CAGAGAGATG CTCTGGTGGC CCTGTTGGAG 
GAGCAGAGAC TGCGGGAGAA AGAGGAGGAC AAAGATCTGG AGGAGGTCAT GTTATCCAAG 
GGGTTTAACC TCAACTGGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814821.2
CDS	357..6980
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959367.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959367.3

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGT TGATCCCGTT GCCGGGACTC CGCGCCGTAG ACCTCTCTCC 
TTCTGCCCCT GCTGTGCCCA CGAAGGGATG GGAAAGAAGG ACGACCCGAA ACTCATGCAG 
GAATGGTTCC GCCTGGTGCA GGAGAAGAAC GCGCTGGTGC GATACGAATC GGAGCTGATG 
ATATTTGCCC GCGAGTTGGA GTTGGAGGAC AGACAGAGCC GACTTCAGCA GGAGCTGCGG 
GAGCGAATGG CAGTGGAAGA TCAATTGAAG ACGGAGCAGG AGCTGACGGA CGAGAAGAAG 
ATCCTGAACG AGATGCTGGA CGTGGTGGAA CAGAGAGATG CTCTGGTGGC CCTGTTGGAG 
GAGCAGAGAC TGCGGGAGAA AGAGGAGGAC AAAGATCTGG AGGAGGTCAT GTTATCCAAG 
GGGTTTAACC TCAACTGGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898582.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754442.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X13, mRNA.

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CloneID	OXa54216
Clone ID Related Accession (Same CDS sequence)	XM_031898582.1
Accession Version	XM_031898582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGAAAA CCTACACGGA CGAGGAGCTA AGCGCTAAGC TAACAAGGCG GGTGCAGAAA 
GCGGCACGGA GACAGGCCAA GCAGGAGGAG CTAAAGCGGC TGCATCGGGC ACAGATCATT 
CAGCGCCAGC TGGAACAAGT GGAGGAAAAG CAGCGGCAGC TGGAGGAGCG AGGGGTGGCT 
GTGGAGAAAG CTCTCCGGGG AGAAGCAGAC TATTGGGGAG AGTCTTATTA CAGTGACCTC 
ATCGATTTGC ACCTGGGTGT TGATCCCGTT GCCGGGACTC CGCGCCGTAG ACCTCTCTCC 
TTCTGCCCCT GCTGTGCCCA CGAAGGGATG GGAAAGAAGG ACGACCCGAA ACTCATGCAG 
GAATGGTTCC GCCTGGTGCA GGAGAAGAAC GCGCTGGTGC GATACGAATC GGAGCTGATG 
ATATTTGCCC GCGAGTTGGA GTTGGAGGAC AGACAGAGCC GACTTCAGCA GGAGCTGCGG 
GAGCGAATGG CAGTGGAAGA TCAATTGAAG ACGGAGCAGG AGCTGACGGA CGAGAAGAAG 
ATCCTGAACG AGATGCTGGA CGTGGTGGAA CAGAGAGATG CTCTGGTGGC CCTGTTGGAG 
GAGCAGAGAC TGCGGGAGAA AGAGGAGGAC AAAGATCTGG AGGAGGTCAT GTTATCCAAG 
GGGTTTAACC TCAACTGGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754442.1
CDS	358..6981
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKREKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898582.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898582.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGAAAA CCTACACGGA CGAGGAGCTA AGCGCTAAGC TAACAAGGCG GGTGCAGAAA 
GCGGCACGGA GACAGGCCAA GCAGGAGGAG CTAAAGCGGC TGCATCGGGC ACAGATCATT 
CAGCGCCAGC TGGAACAAGT GGAGGAAAAG CAGCGGCAGC TGGAGGAGCG AGGGGTGGCT 
GTGGAGAAAG CTCTCCGGGG AGAAGCAGAC TATTGGGGAG AGTCTTATTA CAGTGACCTC 
ATCGATTTGC ACCTGGGTGT TGATCCCGTT GCCGGGACTC CGCGCCGTAG ACCTCTCTCC 
TTCTGCCCCT GCTGTGCCCA CGAAGGGATG GGAAAGAAGG ACGACCCGAA ACTCATGCAG 
GAATGGTTCC GCCTGGTGCA GGAGAAGAAC GCGCTGGTGC GATACGAATC GGAGCTGATG 
ATATTTGCCC GCGAGTTGGA GTTGGAGGAC AGACAGAGCC GACTTCAGCA GGAGCTGCGG 
GAGCGAATGG CAGTGGAAGA TCAATTGAAG ACGGAGCAGG AGCTGACGGA CGAGAAGAAG 
ATCCTGAACG AGATGCTGGA CGTGGTGGAA CAGAGAGATG CTCTGGTGGC CCTGTTGGAG 
GAGCAGAGAC TGCGGGAGAA AGAGGAGGAC AAAGATCTGG AGGAGGTCAT GTTATCCAAG 
GGGTTTAACC TCAACTGGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898583.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754443.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X14, mRNA.

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CloneID	OXa54217
Clone ID Related Accession (Same CDS sequence)	XM_031898583.1
Accession Version	XM_031898583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGGGATGGG AAAGAAGGAC GACCCGAAAC TCATGCAGGA ATGGTTCCGC 
CTGGTGCAGG AGAAGAACGC GCTGGTGCGA TACGAATCGG AGCTGATGAT ATTTGCCCGC 
GAGTTGGAGT TGGAGGACAG ACAGAGCCGA CTTCAGCAGG AGCTGCGGGA GCGAATGGCA 
GTGGAAGATC AATTGAAGAC GGAGCAGGAG CTGACGGACG AGAAGAAGAT CCTGAACGAG 
ATGCTGGACG TGGTGGAACA GAGAGATGCT CTGGTGGCCC TGTTGGAGGA GCAGAGACTG 
CGGGAGAAAG AGGAGGACAA AGATCTGGAG GAGGTCATGT TATCCAAGGG GTTTAACCTC 
AACTGGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754443.1
CDS	357..6968
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898583.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898583.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGCGGT TTCCTGAAGC CTCGTGCCGG GCCCCTGAAT TTCACATGCA CAGAGATGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGGGATGGG AAAGAAGGAC GACCCGAAAC TCATGCAGGA ATGGTTCCGC 
CTGGTGCAGG AGAAGAACGC GCTGGTGCGA TACGAATCGG AGCTGATGAT ATTTGCCCGC 
GAGTTGGAGT TGGAGGACAG ACAGAGCCGA CTTCAGCAGG AGCTGCGGGA GCGAATGGCA 
GTGGAAGATC AATTGAAGAC GGAGCAGGAG CTGACGGACG AGAAGAAGAT CCTGAACGAG 
ATGCTGGACG TGGTGGAACA GAGAGATGCT CTGGTGGCCC TGTTGGAGGA GCAGAGACTG 
CGGGAGAAAG AGGAGGACAA AGATCTGGAG GAGGTCATGT TATCCAAGGG GTTTAACCTC 
AACTGGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959371.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814825.2 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X15, mRNA.

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CloneID	OXa54218
Clone ID Related Accession (Same CDS sequence)	XM_012959371.3
Accession Version	XM_012959371.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959371.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGA CTATTGGGGA GAGTCTTATT ACAGTGACCT CATCGATTTG 
CACCTGGGTG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814825.2
CDS	357..6965
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959371.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959371.3

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGA CTATTGGGGA GAGTCTTATT ACAGTGACCT CATCGATTTG 
CACCTGGGTG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898584.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754444.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X16, mRNA.

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CloneID	OXa54219
Clone ID Related Accession (Same CDS sequence)	XM_031898584.1
Accession Version	XM_031898584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGCCGGGAAC 
CTCAGACTCC AGCAAGCCCT CTCCCCTGTT GACCCCACGG AAATAAAGGC AGACGTGCGC 
TGGACACACG TCCGCGAGGT TAAGGAAGAG CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA 
GTGAGCATGT CCTCTCCTTC CAAAGCTACT GGCGAGTATG AAGAAGGTGA GGAGGAGGAG 
CTGGGTACTT GCCTGGCGCA GGCTGAAGCA GCTGGTGATG GGGACACCTC CGCAGACCTT 
GGTGATGATG ATGACATCCC GTCCGACGCC GAGGCCGAAG AACGGCTCCA GCAGTCTCAG 
AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA GAGCCTAAGG TGGAGCAACC CGTCCTGCCA 
GCAATTGCAG ATTCTGAGAG CGTCCCCAGC CCAGTCTCTC TGGCACCGAA AGCCCCCGTG 
CTGTCTCCAA GAAGGACACT CAAAGCGGAA GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG 
GCAGTGACCT CTCCTGGGGG TCGGTTCTTT GCGGATCCGT ATCTGCCATT GGACAGCAGC 
AACAAAGCCT TAGTGGAAGC AAAAACTCCA ACTCCTCTAT CTCCTGTTTG CTCCCAACCA 
GTCCCATCGC AAGAACCTGT TATCCCTACA TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA 
AACACGCGCC CACTTATATC CCCATCAGCT CCTATTTGTT CCCAGCCACT GCCGTCCTCA 
GAGGGTACCC TAAATGCTAC AGACAGGCCC CCCGTGCGTC AGCAGCCATC CCCTCTAATC 
CCCTCTACTT CTTTAACCAA ACTGCCTTTT ACTGGTCACG TCATGGGATC TGAACAATTA 
GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA CGTATTGACC TTATCGAAGA GTTCTGGACA 
AAAAGCGCCG AGATCAGGCG CAGCTTAGGG CTGGCCCCCG TGGACAGAGA TCGGGTACAT 
GAGATCAGTT TCCCTGCCCT AGCTTCAACT CCTCTTCAGC CTCAGGCACC TAAATCCAAA 
CCTGAGGAGC AAAAGTCACC GTCTCTGAAG CCACAACAAG TTGCAAGAAG AGTGAACCCT 
TCCCGGCTGG ACATTGAACT GCTCTCCCCT CTGACTCCAA TATCCCCTTC TGAGAAGGAG 
CTGAAGAGCT CAGATGGTGA GAAGAAGGAT ATCTCCACCA GCTCTGGCTT AGGCCTGAAT 
GGCAGTTCCA TAACACGTAC TGCCGCCAGT GAAAGCTTTA ACACCTCTGA TTCAGCCATG 
CTTACTCCAC CATCCAGCCC TCCACCACCT CCTCCCCAAG GGGAAGAGCC AGCTACTCTA 
CGTAGAAGCC GTACTCCTTG CCAGAATGGT CCAGAATCTA TCCCAGCCCC TGCCCTAGCA 
CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC CCCCGCTGGG AAGAATCTGT ACGTAAGTCA 
TTTGTAGAAA GTGTAGACGA GATCCCCTTT GCCGATGATG TGGAGGACAC GTACGACGAA 
AGAACGGAGA ATTCCAGCCT TCAAGAGAGG TTCTTTACCC CACCAACGAG TCGGGTTAAA 
CCACATAGAA TTGGTTTCTC CAAGGAAAAT GGAGAGATCC CATCTTCTTC GGAGAGAGTG 
CACCACAGAA AAAGGCTCTT GCCCGACGTC ACTCAGGAGG CCAAAGAACG GGCAGAGGAG 
CGGATGAAAG CCCGGGAGAA GTCTGTGAAG AGCCAGGCAC TGAAAGACGC CATAGCACAA 
CAGCGGCACA AAATGAAACA ACTAGAACCA GGGGCAAGAT CGGCCAAAGC TCTGCCATCT 
ACCGTTATGT TCAGCGATTA CCCTGCAGTA TCTCCTCTCC TGAGAACCCA GACCGAGACT 
CCCACCCTGA AGCTGGATAC CGACCAGGAG GTCTTTATAA CCCCCCCAGC AGCTCCTGAC 
GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC GGGAAACCCA AGAAAAGGCC GTCTATATTC 
TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG GGGGAAAGCA GTCGGGCGTC TGATAAACAG 
GCGACGGACG AGAGCACCAA ACCCAAGTCC TTGTGGAAAT CCGTCTTCTC CGGGTATAAG 
AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG GAGGACAAGT CTACTCCGAG CACTCCATCC 
AGCAGCACCA CCGTGGACTC TGGGAAAGCC AAGGTGGCAA ATATGATCCG CGGAGCAGAC 
CTGTCCCTCC GACGTCACCT GAGCTTCTCG GAGGACTCGG ACCTGTCCAG CGACGATGTC 
TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC 
CCTGAATTTC ACATGCACAG AGATGAGTCG GTGTATGTCC CCCACGCCTT GGCATTCAGA 
CGGTCATACT CATCAAAGAA AACCTACACG GACGAGGAGC TAAGCGCTAA GCTAACAAGG 
CGGGTGCAGA AAGCGGCACG GAGACAGGCC AAGCAGGAGG AGCTAAAGCG GCTGCATCGG 
GCACAGATCA TTCAGCGCCA GCTGGAACAA GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG 
CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG GGAGAAGCAG ACTATTGGGG AGAGTCTTAT 
TACAGTGACC TCATCGATTT GCACCTGGGT GTTGATCCCG TTGCCGGGAC TCCGCGCCGT 
AGACCTCTCT CCTTCTGCCC CTGCTGTGCC CACGAAGGGA TGGGAAAGAA GGACGACCCG 
AAACTCATGC AGGAATGGTT CCGCCTGGTG CAGGAGAAGA ACGCGCTGGT GCGATACGAA 
TCGGAGCTGA TGATATTTGC CCGCGAGTTG GAGTTGGAGG ACAGACAGAG CCGACTTCAG 
CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA GATCAATTGA AGACGGAGCA GGAGCTGACG 
GACGAGAAGA AGATCCTGAA CGAGATGCTG GACGTGGTGG AACAGAGAGA TGCTCTGGTG 
GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG AAAGAGGAGG ACAAAGATCT GGAGGAGGTC 
ATGTTATCCA AGGGGTTTAA CCTCAACTGG TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754444.1
CDS	357..6932
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898584.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898584.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGCCGGGAAC 
CTCAGACTCC AGCAAGCCCT CTCCCCTGTT GACCCCACGG AAATAAAGGC AGACGTGCGC 
TGGACACACG TCCGCGAGGT TAAGGAAGAG CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA 
GTGAGCATGT CCTCTCCTTC CAAAGCTACT GGCGAGTATG AAGAAGGTGA GGAGGAGGAG 
CTGGGTACTT GCCTGGCGCA GGCTGAAGCA GCTGGTGATG GGGACACCTC CGCAGACCTT 
GGTGATGATG ATGACATCCC GTCCGACGCC GAGGCCGAAG AACGGCTCCA GCAGTCTCAG 
AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA GAGCCTAAGG TGGAGCAACC CGTCCTGCCA 
GCAATTGCAG ATTCTGAGAG CGTCCCCAGC CCAGTCTCTC TGGCACCGAA AGCCCCCGTG 
CTGTCTCCAA GAAGGACACT CAAAGCGGAA GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG 
GCAGTGACCT CTCCTGGGGG TCGGTTCTTT GCGGATCCGT ATCTGCCATT GGACAGCAGC 
AACAAAGCCT TAGTGGAAGC AAAAACTCCA ACTCCTCTAT CTCCTGTTTG CTCCCAACCA 
GTCCCATCGC AAGAACCTGT TATCCCTACA TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA 
AACACGCGCC CACTTATATC CCCATCAGCT CCTATTTGTT CCCAGCCACT GCCGTCCTCA 
GAGGGTACCC TAAATGCTAC AGACAGGCCC CCCGTGCGTC AGCAGCCATC CCCTCTAATC 
CCCTCTACTT CTTTAACCAA ACTGCCTTTT ACTGGTCACG TCATGGGATC TGAACAATTA 
GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA CGTATTGACC TTATCGAAGA GTTCTGGACA 
AAAAGCGCCG AGATCAGGCG CAGCTTAGGG CTGGCCCCCG TGGACAGAGA TCGGGTACAT 
GAGATCAGTT TCCCTGCCCT AGCTTCAACT CCTCTTCAGC CTCAGGCACC TAAATCCAAA 
CCTGAGGAGC AAAAGTCACC GTCTCTGAAG CCACAACAAG TTGCAAGAAG AGTGAACCCT 
TCCCGGCTGG ACATTGAACT GCTCTCCCCT CTGACTCCAA TATCCCCTTC TGAGAAGGAG 
CTGAAGAGCT CAGATGGTGA GAAGAAGGAT ATCTCCACCA GCTCTGGCTT AGGCCTGAAT 
GGCAGTTCCA TAACACGTAC TGCCGCCAGT GAAAGCTTTA ACACCTCTGA TTCAGCCATG 
CTTACTCCAC CATCCAGCCC TCCACCACCT CCTCCCCAAG GGGAAGAGCC AGCTACTCTA 
CGTAGAAGCC GTACTCCTTG CCAGAATGGT CCAGAATCTA TCCCAGCCCC TGCCCTAGCA 
CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC CCCCGCTGGG AAGAATCTGT ACGTAAGTCA 
TTTGTAGAAA GTGTAGACGA GATCCCCTTT GCCGATGATG TGGAGGACAC GTACGACGAA 
AGAACGGAGA ATTCCAGCCT TCAAGAGAGG TTCTTTACCC CACCAACGAG TCGGGTTAAA 
CCACATAGAA TTGGTTTCTC CAAGGAAAAT GGAGAGATCC CATCTTCTTC GGAGAGAGTG 
CACCACAGAA AAAGGCTCTT GCCCGACGTC ACTCAGGAGG CCAAAGAACG GGCAGAGGAG 
CGGATGAAAG CCCGGGAGAA GTCTGTGAAG AGCCAGGCAC TGAAAGACGC CATAGCACAA 
CAGCGGCACA AAATGAAACA ACTAGAACCA GGGGCAAGAT CGGCCAAAGC TCTGCCATCT 
ACCGTTATGT TCAGCGATTA CCCTGCAGTA TCTCCTCTCC TGAGAACCCA GACCGAGACT 
CCCACCCTGA AGCTGGATAC CGACCAGGAG GTCTTTATAA CCCCCCCAGC AGCTCCTGAC 
GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC GGGAAACCCA AGAAAAGGCC GTCTATATTC 
TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG GGGGAAAGCA GTCGGGCGTC TGATAAACAG 
GCGACGGACG AGAGCACCAA ACCCAAGTCC TTGTGGAAAT CCGTCTTCTC CGGGTATAAG 
AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG GAGGACAAGT CTACTCCGAG CACTCCATCC 
AGCAGCACCA CCGTGGACTC TGGGAAAGCC AAGGTGGCAA ATATGATCCG CGGAGCAGAC 
CTGTCCCTCC GACGTCACCT GAGCTTCTCG GAGGACTCGG ACCTGTCCAG CGACGATGTC 
TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC 
CCTGAATTTC ACATGCACAG AGATGAGTCG GTGTATGTCC CCCACGCCTT GGCATTCAGA 
CGGTCATACT CATCAAAGAA AACCTACACG GACGAGGAGC TAAGCGCTAA GCTAACAAGG 
CGGGTGCAGA AAGCGGCACG GAGACAGGCC AAGCAGGAGG AGCTAAAGCG GCTGCATCGG 
GCACAGATCA TTCAGCGCCA GCTGGAACAA GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG 
CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG GGAGAAGCAG ACTATTGGGG AGAGTCTTAT 
TACAGTGACC TCATCGATTT GCACCTGGGT GTTGATCCCG TTGCCGGGAC TCCGCGCCGT 
AGACCTCTCT CCTTCTGCCC CTGCTGTGCC CACGAAGGGA TGGGAAAGAA GGACGACCCG 
AAACTCATGC AGGAATGGTT CCGCCTGGTG CAGGAGAAGA ACGCGCTGGT GCGATACGAA 
TCGGAGCTGA TGATATTTGC CCGCGAGTTG GAGTTGGAGG ACAGACAGAG CCGACTTCAG 
CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA GATCAATTGA AGACGGAGCA GGAGCTGACG 
GACGAGAAGA AGATCCTGAA CGAGATGCTG GACGTGGTGG AACAGAGAGA TGCTCTGGTG 
GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG AAAGAGGAGG ACAAAGATCT GGAGGAGGTC 
ATGTTATCCA AGGGGTTTAA CCTCAACTGG TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959375.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814829.2 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X17, mRNA.

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CloneID	OXa54220
Clone ID Related Accession (Same CDS sequence)	XM_012959375.3
Accession Version	XM_012959375.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959375.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGAAAA CCTACACGGA CGAGGAGCTA AGCGCTAAGC TAACAAGGCG GGTGCAGAAA 
GCGGCACGGA GACAGGCCAA GCAGGAGGAG CTAAAGCGGC TGCATCGGGC ACAGATCATT 
CAGCGCCAGC TGGAACAAGT GGAGGAAAAG CAGCGGCAGC TGGAGGAGCG AGGGGTGGCT 
GTGGAGAAAG CTCTCCGGGG AGAAGCAGAC TATTGGGGAG AGTCTTATTA CAGTGACCTC 
ATCGATTTGC ACCTGGGTGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814829.2
CDS	357..6914
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKREKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959375.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959375.3

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC CTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCTGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGAAAA CCTACACGGA CGAGGAGCTA AGCGCTAAGC TAACAAGGCG GGTGCAGAAA 
GCGGCACGGA GACAGGCCAA GCAGGAGGAG CTAAAGCGGC TGCATCGGGC ACAGATCATT 
CAGCGCCAGC TGGAACAAGT GGAGGAAAAG CAGCGGCAGC TGGAGGAGCG AGGGGTGGCT 
GTGGAGAAAG CTCTCCGGGG AGAAGCAGAC TATTGGGGAG AGTCTTATTA CAGTGACCTC 
ATCGATTTGC ACCTGGGTGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898585.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754445.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X18, mRNA.

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CloneID	OXa54221
Clone ID Related Accession (Same CDS sequence)	XM_031898585.1
Accession Version	XM_031898585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGCGCGTCAT 
CTCCATTCCC ACCGTCACGA CCCAGTGAGA GCGTGGCTGG AGTCCGTATC AGGAGCTACT 
GGCGAGTATG AAGAAGGTGA GGAGGAGGAG CTGGGTACTT GCCTGGCGCA GGCTGAAGCA 
GCTGGTGATG GGGACACCTC CGCAGACCTT GGTGATGATG ATGACATCCC GTCCGACGCC 
GAGGCCGAAG AACGGCTCCA GCAGTCTCAG AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA 
GAGCCTAAGG TGGAGCAACC CGTCCTGCCA GCAATTGCAG ATTCTGAGAG CGTCCCCAGC 
CCAGTCTCTC TGGCACCGAA AGCCCCCGTG CTGTCTCCAA GAAGGACACT CAAAGCGGAA 
GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG GCAGTGACCT CTCCTGGGGG TCGGTTCTTT 
GCGGATCCGT ATCTGCCATT GGACAGCAGC AACAAAGCCT TAGTGGAAGC AAAAACTCCA 
ACTCCTCTAT CTCCTGTTTG CTCCCAACCA GTCCCATCGC AAGAACCTGT TATCCCTACA 
TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA AACACGCGCC CACTTATATC CCCATCAGCT 
CCTATTTGTT CCCAGCCACT GCCGTCCTCA GAGGGTACCC TAAATGCTAC AGACAGGCCC 
CCCGTGCGTC AGCAGCCATC CCCTCTAATC CCCTCTACTT CTTTAACCAA ACTGCCTTTT 
ACTGGTCACG TCATGGGATC TGAACAATTA GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA 
CGTATTGACC TTATCGAAGA GTTCTGGACA AAAAGCGCCG AGATCAGGCG CAGCTTAGGG 
CTGGCCCCCG TGGACAGAGA TCGGGTACAT GAGATCAGTT TCCCTGCCCT AGCTTCAACT 
CCTCTTCAGC CTCAGGCACC TAAATCCAAA CCTGAGGAGC AAAAGTCACC GTCTCTGAAG 
CCACAACAAG TTGCAAGAAG AGTGAACCCT TCCCGGCTGG ACATTGAACT GCTCTCCCCT 
CTGACTCCAA TATCCCCTTC TGAGAAGGAG CTGAAGAGCT CAGATGGTGA GAAGAAGGAT 
ATCTCCACCA GCTCTGGCTT AGGCCTGAAT GGCAGTTCCA TAACACGTAC TGCCGCCAGT 
GAAAGCTTTA ACACCTCTGA TTCAGCCATG CTTACTCCAC CATCCAGCCC TCCACCACCT 
CCTCCCCAAG GGGAAGAGCC AGCTACTCTA CGTAGAAGCC GTACTCCTTG CCAGAATGGT 
CCAGAATCTA TCCCAGCCCC TGCCCTAGCA CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC 
CCCCGCTGGG AAGAATCTGT ACGTAAGTCA TTTGTAGAAA GTGTAGACGA GATCCCCTTT 
GCCGATGATG TGGAGGACAC GTACGACGAA AGAACGGAGA ATTCCAGCCT TCAAGAGAGG 
TTCTTTACCC CACCAACGAG TCGGGTTAAA CCACATAGAA TTGGTTTCTC CAAGGAAAAT 
GGAGAGATCC CATCTTCTTC GGAGAGAGTG CACCACAGAA AAAGGCTCTT GCCCGACGTC 
ACTCAGGAGG CCAAAGAACG GGCAGAGGAG CGGATGAAAG CCCGGGAGAA GTCTGTGAAG 
AGCCAGGCAC TGAAAGACGC CATAGCACAA CAGCGGCACA AAATGAAACA ACTAGAACCA 
GGGGCAAGAT CGGCCAAAGC TCTGCCATCT ACCGTTATGT TCAGCGATTA CCCTGCAGTA 
TCTCCTCTCC TGAGAACCCA GACCGAGACT CCCACCCTGA AGCTGGATAC CGACCAGGAG 
GTCTTTATAA CCCCCCCAGC AGCTCCTGAC GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC 
GGGAAACCCA AGAAAAGGCC GTCTATATTC TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG 
GGGGAAAGCA GTCGGGCGTC TGATAAACAG GCGACGGACG AGAGCACCAA ACCCAAGTCC 
TTGTGGAAAT CCGTCTTCTC CGGGTATAAG AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG 
GAGGACAAGT CTACTCCGAG CACTCCATCC AGCAGCACCA CCGTGGACTC TGGGAAAGCC 
AAGGTGGCAA ATATGATCCG CGGAGCAGAC CTGTCCCTCC GACGTCACCT GAGCTTCTCG 
GAGGACTCGG ACCTGTCCAG CGACGATGTC TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA 
GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC CCTGAATTTC ACATGCACAG AGATGAGTCG 
GTGTATGTCC CCCACGCCTT GGCATTCAGA CGGTCATACT CATCAAAGAA AACCTACACG 
GACGAGGAGC TAAGCGCTAA GCTAACAAGG CGGGTGCAGA AAGCGGCACG GAGACAGGCC 
AAGCAGGAGG AGCTAAAGCG GCTGCATCGG GCACAGATCA TTCAGCGCCA GCTGGAACAA 
GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG 
GGAGAAGCAG ACTATTGGGG AGAGTCTTAT TACAGTGACC TCATCGATTT GCACCTGGGT 
GTTGATCCCG TTGCCGGGAC TCCGCGCCGT AGACCTCTCT CCTTCTGCCC CTGCTGTGCC 
CACGAAGGGA TGGGAAAGAA GGACGACCCG AAACTCATGC AGGAATGGTT CCGCCTGGTG 
CAGGAGAAGA ACGCGCTGGT GCGATACGAA TCGGAGCTGA TGATATTTGC CCGCGAGTTG 
GAGTTGGAGG ACAGACAGAG CCGACTTCAG CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA 
GATCAATTGA AGACGGAGCA GGAGCTGACG GACGAGAAGA AGATCCTGAA CGAGATGCTG 
GACGTGGTGG AACAGAGAGA TGCTCTGGTG GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG 
AAAGAGGAGG ACAAAGATCT GGAGGAGGTC ATGTTATCCA AGGGGTTTAA CCTCAACTGG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754445.1
CDS	356..6841
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898585.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898585.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGCGCGTCAT 
CTCCATTCCC ACCGTCACGA CCCAGTGAGA GCGTGGCTGG AGTCCGTATC AGGAGCTACT 
GGCGAGTATG AAGAAGGTGA GGAGGAGGAG CTGGGTACTT GCCTGGCGCA GGCTGAAGCA 
GCTGGTGATG GGGACACCTC CGCAGACCTT GGTGATGATG ATGACATCCC GTCCGACGCC 
GAGGCCGAAG AACGGCTCCA GCAGTCTCAG AGGTTTAAAT TGAAGCTGGA TGTAGCCCAA 
GAGCCTAAGG TGGAGCAACC CGTCCTGCCA GCAATTGCAG ATTCTGAGAG CGTCCCCAGC 
CCAGTCTCTC TGGCACCGAA AGCCCCCGTG CTGTCTCCAA GAAGGACACT CAAAGCGGAA 
GCAGAAAAGG TTCCTGTGAT GTCTCCGAAG GCAGTGACCT CTCCTGGGGG TCGGTTCTTT 
GCGGATCCGT ATCTGCCATT GGACAGCAGC AACAAAGCCT TAGTGGAAGC AAAAACTCCA 
ACTCCTCTAT CTCCTGTTTG CTCCCAACCA GTCCCATCGC AAGAACCTGT TATCCCTACA 
TCTCCTGTGA AGTCTCCCTC AGTCCCAGCA AACACGCGCC CACTTATATC CCCATCAGCT 
CCTATTTGTT CCCAGCCACT GCCGTCCTCA GAGGGTACCC TAAATGCTAC AGACAGGCCC 
CCCGTGCGTC AGCAGCCATC CCCTCTAATC CCCTCTACTT CTTTAACCAA ACTGCCTTTT 
ACTGGTCACG TCATGGGATC TGAACAATTA GTTAGCCCTA ATGCAAAAGA GGTCCTGAAA 
CGTATTGACC TTATCGAAGA GTTCTGGACA AAAAGCGCCG AGATCAGGCG CAGCTTAGGG 
CTGGCCCCCG TGGACAGAGA TCGGGTACAT GAGATCAGTT TCCCTGCCCT AGCTTCAACT 
CCTCTTCAGC CTCAGGCACC TAAATCCAAA CCTGAGGAGC AAAAGTCACC GTCTCTGAAG 
CCACAACAAG TTGCAAGAAG AGTGAACCCT TCCCGGCTGG ACATTGAACT GCTCTCCCCT 
CTGACTCCAA TATCCCCTTC TGAGAAGGAG CTGAAGAGCT CAGATGGTGA GAAGAAGGAT 
ATCTCCACCA GCTCTGGCTT AGGCCTGAAT GGCAGTTCCA TAACACGTAC TGCCGCCAGT 
GAAAGCTTTA ACACCTCTGA TTCAGCCATG CTTACTCCAC CATCCAGCCC TCCACCACCT 
CCTCCCCAAG GGGAAGAGCC AGCTACTCTA CGTAGAAGCC GTACTCCTTG CCAGAATGGT 
CCAGAATCTA TCCCAGCCCC TGCCCTAGCA CAGAGCAAGC CCCCTGAGCC CAAAAAGTCC 
CCCCGCTGGG AAGAATCTGT ACGTAAGTCA TTTGTAGAAA GTGTAGACGA GATCCCCTTT 
GCCGATGATG TGGAGGACAC GTACGACGAA AGAACGGAGA ATTCCAGCCT TCAAGAGAGG 
TTCTTTACCC CACCAACGAG TCGGGTTAAA CCACATAGAA TTGGTTTCTC CAAGGAAAAT 
GGAGAGATCC CATCTTCTTC GGAGAGAGTG CACCACAGAA AAAGGCTCTT GCCCGACGTC 
ACTCAGGAGG CCAAAGAACG GGCAGAGGAG CGGATGAAAG CCCGGGAGAA GTCTGTGAAG 
AGCCAGGCAC TGAAAGACGC CATAGCACAA CAGCGGCACA AAATGAAACA ACTAGAACCA 
GGGGCAAGAT CGGCCAAAGC TCTGCCATCT ACCGTTATGT TCAGCGATTA CCCTGCAGTA 
TCTCCTCTCC TGAGAACCCA GACCGAGACT CCCACCCTGA AGCTGGATAC CGACCAGGAG 
GTCTTTATAA CCCCCCCAGC AGCTCCTGAC GGCTCCGTCA CTTCCTCGGA AGGTTCAGGC 
GGGAAACCCA AGAAAAGGCC GTCTATATTC TCCCCGAGGA AGAAAGAGAA AAAGTCCAAG 
GGGGAAAGCA GTCGGGCGTC TGATAAACAG GCGACGGACG AGAGCACCAA ACCCAAGTCC 
TTGTGGAAAT CCGTCTTCTC CGGGTATAAG AAGGAAAAGA AGAAGAAGCA GCAGCAGGCG 
GAGGACAAGT CTACTCCGAG CACTCCATCC AGCAGCACCA CCGTGGACTC TGGGAAAGCC 
AAGGTGGCAA ATATGATCCG CGGAGCAGAC CTGTCCCTCC GACGTCACCT GAGCTTCTCG 
GAGGACTCGG ACCTGTCCAG CGACGATGTC TTGGAAAAAT CCTCCCAGAA ATCTAAGCGA 
GAGCGGTTTC CTGAAGCCTC GTGCCGGGCC CCTGAATTTC ACATGCACAG AGATGAGTCG 
GTGTATGTCC CCCACGCCTT GGCATTCAGA CGGTCATACT CATCAAAGAA AACCTACACG 
GACGAGGAGC TAAGCGCTAA GCTAACAAGG CGGGTGCAGA AAGCGGCACG GAGACAGGCC 
AAGCAGGAGG AGCTAAAGCG GCTGCATCGG GCACAGATCA TTCAGCGCCA GCTGGAACAA 
GTGGAGGAAA AGCAGCGGCA GCTGGAGGAG CGAGGGGTGG CTGTGGAGAA AGCTCTCCGG 
GGAGAAGCAG ACTATTGGGG AGAGTCTTAT TACAGTGACC TCATCGATTT GCACCTGGGT 
GTTGATCCCG TTGCCGGGAC TCCGCGCCGT AGACCTCTCT CCTTCTGCCC CTGCTGTGCC 
CACGAAGGGA TGGGAAAGAA GGACGACCCG AAACTCATGC AGGAATGGTT CCGCCTGGTG 
CAGGAGAAGA ACGCGCTGGT GCGATACGAA TCGGAGCTGA TGATATTTGC CCGCGAGTTG 
GAGTTGGAGG ACAGACAGAG CCGACTTCAG CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA 
GATCAATTGA AGACGGAGCA GGAGCTGACG GACGAGAAGA AGATCCTGAA CGAGATGCTG 
GACGTGGTGG AACAGAGAGA TGCTCTGGTG GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG 
AAAGAGGAGG ACAAAGATCT GGAGGAGGTC ATGTTATCCA AGGGGTTTAA CCTCAACTGG 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898586.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754446.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X19, mRNA.

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CloneID	OXa54222
Clone ID Related Accession (Same CDS sequence)	XM_031898586.1
Accession Version	XM_031898586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGCTACTGGC 
GAGTATGAAG AAGGTGAGGA GGAGGAGCTG GGTACTTGCC TGGCGCAGGC TGAAGCAGCT 
GGTGATGGGG ACACCTCCGC AGACCTTGGT GATGATGATG ACATCCCGTC CGACGCCGAG 
GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG TTTAAATTGA AGCTGGATGT AGCCCAAGAG 
CCTAAGGTGG AGCAACCCGT CCTGCCAGCA ATTGCAGATT CTGAGAGCGT CCCCAGCCCA 
GTCTCTCTGG CACCGAAAGC CCCCGTGCTG TCTCCAAGAA GGACACTCAA AGCGGAAGCA 
GAAAAGGTTC CTGTGATGTC TCCGAAGGCA GTGACCTCTC CTGGGGGTCG GTTCTTTGCG 
GATCCGTATC TGCCATTGGA CAGCAGCAAC AAAGCCTTAG TGGAAGCAAA AACTCCAACT 
CCTCTATCTC CTGTTTGCTC CCAACCAGTC CCATCGCAAG AACCTGTTAT CCCTACATCT 
CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC ACGCGCCCAC TTATATCCCC ATCAGCTCCT 
ATTTGTTCCC AGCCACTGCC GTCCTCAGAG GGTACCCTAA ATGCTACAGA CAGGCCCCCC 
GTGCGTCAGC AGCCATCCCC TCTAATCCCC TCTACTTCTT TAACCAAACT GCCTTTTACT 
GGTCACGTCA TGGGATCTGA ACAATTAGTT AGCCCTAATG CAAAAGAGGT CCTGAAACGT 
ATTGACCTTA TCGAAGAGTT CTGGACAAAA AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG 
GCCCCCGTGG ACAGAGATCG GGTACATGAG ATCAGTTTCC CTGCCCTAGC TTCAACTCCT 
CTTCAGCCTC AGGCACCTAA ATCCAAACCT GAGGAGCAAA AGTCACCGTC TCTGAAGCCA 
CAACAAGTTG CAAGAAGAGT GAACCCTTCC CGGCTGGACA TTGAACTGCT CTCCCCTCTG 
ACTCCAATAT CCCCTTCTGA GAAGGAGCTG AAGAGCTCAG ATGGTGAGAA GAAGGATATC 
TCCACCAGCT CTGGCTTAGG CCTGAATGGC AGTTCCATAA CACGTACTGC CGCCAGTGAA 
AGCTTTAACA CCTCTGATTC AGCCATGCTT ACTCCACCAT CCAGCCCTCC ACCACCTCCT 
CCCCAAGGGG AAGAGCCAGC TACTCTACGT AGAAGCCGTA CTCCTTGCCA GAATGGTCCA 
GAATCTATCC CAGCCCCTGC CCTAGCACAG AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC 
CGCTGGGAAG AATCTGTACG TAAGTCATTT GTAGAAAGTG TAGACGAGAT CCCCTTTGCC 
GATGATGTGG AGGACACGTA CGACGAAAGA ACGGAGAATT CCAGCCTTCA AGAGAGGTTC 
TTTACCCCAC CAACGAGTCG GGTTAAACCA CATAGAATTG GTTTCTCCAA GGAAAATGGA 
GAGATCCCAT CTTCTTCGGA GAGAGTGCAC CACAGAAAAA GGCTCTTGCC CGACGTCACT 
CAGGAGGCCA AAGAACGGGC AGAGGAGCGG ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC 
CAGGCACTGA AAGACGCCAT AGCACAACAG CGGCACAAAA TGAAACAACT AGAACCAGGG 
GCAAGATCGG CCAAAGCTCT GCCATCTACC GTTATGTTCA GCGATTACCC TGCAGTATCT 
CCTCTCCTGA GAACCCAGAC CGAGACTCCC ACCCTGAAGC TGGATACCGA CCAGGAGGTC 
TTTATAACCC CCCCAGCAGC TCCTGACGGC TCCGTCACTT CCTCGGAAGG TTCAGGCGGG 
AAACCCAAGA AAAGGCCGTC TATATTCTCC CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG 
GAAAGCAGTC GGGCGTCTGA TAAACAGGCG ACGGACGAGA GCACCAAACC CAAGTCCTTG 
TGGAAATCCG TCTTCTCCGG GTATAAGAAG GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG 
GACAAGTCTA CTCCGAGCAC TCCATCCAGC AGCACCACCG TGGACTCTGG GAAAGCCAAG 
GTGGCAAATA TGATCCGCGG AGCAGACCTG TCCCTCCGAC GTCACCTGAG CTTCTCGGAG 
GACTCGGACC TGTCCAGCGA CGATGTCTTG GAAAAATCCT CCCAGAAATC TAAGCGAGAG 
CGGTTTCCTG AAGCCTCGTG CCGGGCCCCT GAATTTCACA TGCACAGAGA TGAGTCGGTG 
TATGTCCCCC ACGCCTTGGC ATTCAGACGG TCATACTCAT CAAAGAAAAC CTACACGGAC 
GAGGAGCTAA GCGCTAAGCT AACAAGGCGG GTGCAGAAAG CGGCACGGAG ACAGGCCAAG 
CAGGAGGAGC TAAAGCGGCT GCATCGGGCA CAGATCATTC AGCGCCAGCT GGAACAAGTG 
GAGGAAAAGC AGCGGCAGCT GGAGGAGCGA GGGGTGGCTG TGGAGAAAGC TCTCCGGGGA 
GAAGCAGACT ATTGGGGAGA GTCTTATTAC AGTGACCTCA TCGATTTGCA CCTGGGTGTT 
GATCCCGTTG CCGGGACTCC GCGCCGTAGA CCTCTCTCCT TCTGCCCCTG CTGTGCCCAC 
GAAGGGATGG GAAAGAAGGA CGACCCGAAA CTCATGCAGG AATGGTTCCG CCTGGTGCAG 
GAGAAGAACG CGCTGGTGCG ATACGAATCG GAGCTGATGA TATTTGCCCG CGAGTTGGAG 
TTGGAGGACA GACAGAGCCG ACTTCAGCAG GAGCTGCGGG AGCGAATGGC AGTGGAAGAT 
CAATTGAAGA CGGAGCAGGA GCTGACGGAC GAGAAGAAGA TCCTGAACGA GATGCTGGAC 
GTGGTGGAAC AGAGAGATGC TCTGGTGGCC CTGTTGGAGG AGCAGAGACT GCGGGAGAAA 
GAGGAGGACA AAGATCTGGA GGAGGTCATG TTATCCAAGG GGTTTAACCT CAACTGGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754446.1
CDS	356..6778
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898586.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898586.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGCTACTGGC 
GAGTATGAAG AAGGTGAGGA GGAGGAGCTG GGTACTTGCC TGGCGCAGGC TGAAGCAGCT 
GGTGATGGGG ACACCTCCGC AGACCTTGGT GATGATGATG ACATCCCGTC CGACGCCGAG 
GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG TTTAAATTGA AGCTGGATGT AGCCCAAGAG 
CCTAAGGTGG AGCAACCCGT CCTGCCAGCA ATTGCAGATT CTGAGAGCGT CCCCAGCCCA 
GTCTCTCTGG CACCGAAAGC CCCCGTGCTG TCTCCAAGAA GGACACTCAA AGCGGAAGCA 
GAAAAGGTTC CTGTGATGTC TCCGAAGGCA GTGACCTCTC CTGGGGGTCG GTTCTTTGCG 
GATCCGTATC TGCCATTGGA CAGCAGCAAC AAAGCCTTAG TGGAAGCAAA AACTCCAACT 
CCTCTATCTC CTGTTTGCTC CCAACCAGTC CCATCGCAAG AACCTGTTAT CCCTACATCT 
CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC ACGCGCCCAC TTATATCCCC ATCAGCTCCT 
ATTTGTTCCC AGCCACTGCC GTCCTCAGAG GGTACCCTAA ATGCTACAGA CAGGCCCCCC 
GTGCGTCAGC AGCCATCCCC TCTAATCCCC TCTACTTCTT TAACCAAACT GCCTTTTACT 
GGTCACGTCA TGGGATCTGA ACAATTAGTT AGCCCTAATG CAAAAGAGGT CCTGAAACGT 
ATTGACCTTA TCGAAGAGTT CTGGACAAAA AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG 
GCCCCCGTGG ACAGAGATCG GGTACATGAG ATCAGTTTCC CTGCCCTAGC TTCAACTCCT 
CTTCAGCCTC AGGCACCTAA ATCCAAACCT GAGGAGCAAA AGTCACCGTC TCTGAAGCCA 
CAACAAGTTG CAAGAAGAGT GAACCCTTCC CGGCTGGACA TTGAACTGCT CTCCCCTCTG 
ACTCCAATAT CCCCTTCTGA GAAGGAGCTG AAGAGCTCAG ATGGTGAGAA GAAGGATATC 
TCCACCAGCT CTGGCTTAGG CCTGAATGGC AGTTCCATAA CACGTACTGC CGCCAGTGAA 
AGCTTTAACA CCTCTGATTC AGCCATGCTT ACTCCACCAT CCAGCCCTCC ACCACCTCCT 
CCCCAAGGGG AAGAGCCAGC TACTCTACGT AGAAGCCGTA CTCCTTGCCA GAATGGTCCA 
GAATCTATCC CAGCCCCTGC CCTAGCACAG AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC 
CGCTGGGAAG AATCTGTACG TAAGTCATTT GTAGAAAGTG TAGACGAGAT CCCCTTTGCC 
GATGATGTGG AGGACACGTA CGACGAAAGA ACGGAGAATT CCAGCCTTCA AGAGAGGTTC 
TTTACCCCAC CAACGAGTCG GGTTAAACCA CATAGAATTG GTTTCTCCAA GGAAAATGGA 
GAGATCCCAT CTTCTTCGGA GAGAGTGCAC CACAGAAAAA GGCTCTTGCC CGACGTCACT 
CAGGAGGCCA AAGAACGGGC AGAGGAGCGG ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC 
CAGGCACTGA AAGACGCCAT AGCACAACAG CGGCACAAAA TGAAACAACT AGAACCAGGG 
GCAAGATCGG CCAAAGCTCT GCCATCTACC GTTATGTTCA GCGATTACCC TGCAGTATCT 
CCTCTCCTGA GAACCCAGAC CGAGACTCCC ACCCTGAAGC TGGATACCGA CCAGGAGGTC 
TTTATAACCC CCCCAGCAGC TCCTGACGGC TCCGTCACTT CCTCGGAAGG TTCAGGCGGG 
AAACCCAAGA AAAGGCCGTC TATATTCTCC CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG 
GAAAGCAGTC GGGCGTCTGA TAAACAGGCG ACGGACGAGA GCACCAAACC CAAGTCCTTG 
TGGAAATCCG TCTTCTCCGG GTATAAGAAG GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG 
GACAAGTCTA CTCCGAGCAC TCCATCCAGC AGCACCACCG TGGACTCTGG GAAAGCCAAG 
GTGGCAAATA TGATCCGCGG AGCAGACCTG TCCCTCCGAC GTCACCTGAG CTTCTCGGAG 
GACTCGGACC TGTCCAGCGA CGATGTCTTG GAAAAATCCT CCCAGAAATC TAAGCGAGAG 
CGGTTTCCTG AAGCCTCGTG CCGGGCCCCT GAATTTCACA TGCACAGAGA TGAGTCGGTG 
TATGTCCCCC ACGCCTTGGC ATTCAGACGG TCATACTCAT CAAAGAAAAC CTACACGGAC 
GAGGAGCTAA GCGCTAAGCT AACAAGGCGG GTGCAGAAAG CGGCACGGAG ACAGGCCAAG 
CAGGAGGAGC TAAAGCGGCT GCATCGGGCA CAGATCATTC AGCGCCAGCT GGAACAAGTG 
GAGGAAAAGC AGCGGCAGCT GGAGGAGCGA GGGGTGGCTG TGGAGAAAGC TCTCCGGGGA 
GAAGCAGACT ATTGGGGAGA GTCTTATTAC AGTGACCTCA TCGATTTGCA CCTGGGTGTT 
GATCCCGTTG CCGGGACTCC GCGCCGTAGA CCTCTCTCCT TCTGCCCCTG CTGTGCCCAC 
GAAGGGATGG GAAAGAAGGA CGACCCGAAA CTCATGCAGG AATGGTTCCG CCTGGTGCAG 
GAGAAGAACG CGCTGGTGCG ATACGAATCG GAGCTGATGA TATTTGCCCG CGAGTTGGAG 
TTGGAGGACA GACAGAGCCG ACTTCAGCAG GAGCTGCGGG AGCGAATGGC AGTGGAAGAT 
CAATTGAAGA CGGAGCAGGA GCTGACGGAC GAGAAGAAGA TCCTGAACGA GATGCTGGAC 
GTGGTGGAAC AGAGAGATGC TCTGGTGGCC CTGTTGGAGG AGCAGAGACT GCGGGAGAAA 
GAGGAGGACA AAGATCTGGA GGAGGTCATG TTATCCAAGG GGTTTAACCT CAACTGGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898587.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754447.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X20, mRNA.

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CloneID	OXa54223
Clone ID Related Accession (Same CDS sequence)	XM_031898587.1
Accession Version	XM_031898587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG GGCTCATTTA GGAAAGAGTT CCCCCAGAAC 
CTGGGAGGGA GCGACGTCTG CTATTTCTGC CGCCGGAGGG TTTACGTCAT GGAGCGGCTC 
AGCGCCGAGG GCAAGTTCTT CCACCGGAGC TGCTTTAAGT GTGACTACTG TAGCACAACG 
CTGCGCCTCT CATGTTATGC CTATGACCTT GAGGATGGAA AATTTTACTG CAAACCTCAC 
TACTGTTACC GCCGGTCCGG ATACAGCCAG AGGAAGAGGC CGGCCATGCC TGCACTTTCG 
GGAAAAGAAG TCACAAGGAC TGTGGAAGAC ACCCCCGACG CGGAAGACCA AGGACGAGCC 
CCGCTGCTGC CCGCCCCCGC TGCTGAGAAG ACCCCAGGCC CCAGTGTGAA CGGCGTGGAA 
GATCACAGCG CCGCCAAGAG GCTGAGAGCC ACCCCAGAGC GCATTGAGCT TGAGAATTAC 
CGCGTCTCCC TGCAGAAGGA GGAGCTGGAG GAGGTGCCCG AGGAAACCCT GGCGGAGCAT 
AATCTGAGCA GCCTATTGGA TAAAGGGGAG GTGGAAGAGG TGGCCAGCAG CAGTTCCGAG 
TCCGAGATGG AGGAGGAGGA GGACGAAGAA GAAGAGGAGG AGGAGCCAGG AGAGTGCCTT 
CCCCCCTCAG ATCTGGGTGG GGTGCCGTGG AAAGAGGCCG TCAAGATCCA CGCCAAGTTA 
AAGGGTCAGG ACATAGATGT GGAGGTTGTG GGAACCCAGG GGGCTGGTGG CAGTGAAGAG 
GAGGAGGAGG AGGAGGAAGA AGAGGACGAA GAGGAGGAAG ATGAAGAGGT ATCAAGTGAA 
GAGGGTGAGT ACTGCCCATG GGAGAGAGAG CTTCAGCAGG GCCTGTGGAT ACAGCGTATC 
TCTGATGAAG ATACAGTTGG TATATTTAAA GCCGGGAACC TCAGACTCCA GCAAGCCCTC 
TCCCCTGTTG ACCCCACGGA AATAAAGGCA GACGTGCGCT GGACACACGT CCGCGAGGTT 
AAGGAAGAGC GGAGAGGGTC TAAGGAAGAG AGGGGAGAAG TGAGCATGTC CTCTCCTTCC 
AAAGCGCGTC ATCTCCATTC CCACCGTCAC GACCCAGTGA GAGCGTGGCT GGAGTCCGTA 
TCAGGAGCTA CTGGCGAGTA TGAAGAAGGT GAGGAGGAGG AGCTGGGTAC TTGCCTGGCG 
CAGGCTGAAG CAGCTGGTGA TGGGGACACC TCCGCAGACC TTGGTGATGA TGATGACATC 
CCGTCCGACG CCGAGGCCGA AGAACGGCTC CAGCAGTCTC AGAGGTTTAA ATTGAAGCTG 
GATGTAGCCC AAGAGCCTAA GGTGGAGCAA CCCGTCCTGC CAGCAATTGC AGATTCTGAG 
AGCGTCCCCA GCCCAGTCTC TCTGGCACCG AAAGCCCCCG TGCTGTCTCC AAGAAGGACA 
CTCAAAGCGG AAGCAGAAAA GGTTCCTGTG ATGTCTCCGA AGGCAGTGAC CTCTCCTGGG 
GGTCGGTTCT TTGCGGATCC GTATCTGCCA TTGGACAGCA GCAACAAAGC CTTAGTGGAA 
GCAAAAACTC CAACTCCTCT ATCTCCTGTT TGCTCCCAAC CAGTCCCATC GCAAGAACCT 
GTTATCCCTA CATCTCCTGT GAAGTCTCCC TCAGTCCCAG CAAACACGCG CCCACTTATA 
TCCCCATCAG CTCCTATTTG TTCCCAGCCA CTGCCGTCCT CAGAGGGTAC CCTAAATGCT 
ACAGACAGGC CCCCCGTGCG TCAGCAGCCA TCCCCTCTAA TCCCCTCTAC TTCTTTAACC 
AAACTGCCTT TTACTGGTCA CGTCATGGGA TCTGAACAAT TAGTTAGCCC TAATGCAAAA 
GAGGTCCTGA AACGTATTGA CCTTATCGAA GAGTTCTGGA CAAAAAGCGC CGAGATCAGG 
CGCAGCTTAG GGCTGGCCCC CGTGGACAGA GATCGGGTAC ATGAGATCAG TTTCCCTGCC 
CTAGCTTCAA CTCCTCTTCA GCCTCAGGCA CCTAAATCCA AACCTGAGGA GCAAAAGTCA 
CCGTCTCTGA AGCCACAACA AGTTGCAAGA AGAGTGAACC CTTCCCGGCT GGACATTGAA 
CTGCTCTCCC CTCTGACTCC AATATCCCCT TCTGAGAAGG AGCTGAAGAG CTCAGATGGT 
GAGAAGAAGG ATATCTCCAC CAGCTCTGGC TTAGGCCTGA ATGGCAGTTC CATAACACGT 
ACTGCCGCCA GTGAAAGCTT TAACACCTCT GATTCAGCCA TGCTTACTCC ACCATCCAGC 
CCTCCACCAC CTCCTCCCCA AGGGGAAGAG CCAGCTACTC TACGTAGAAG CCGTACTCCT 
TGCCAGAATG GTCCAGAATC TATCCCAGCC CCTGCCCTAG CACAGAGCAA GCCCCCTGAG 
CCCAAAAAGT CCCCCCGCTG GGAAGAATCT GTACGTAAGT CATTTGTAGA AAGTGTAGAC 
GAGATCCCCT TTGCCGATGA TGTGGAGGAC ACGTACGACG AAAGAACGGA GAATTCCAGC 
CTTCAAGAGA GGTTCTTTAC CCCACCAACG AGTCGGGTTA AACCACATAG AATTGGTTTC 
TCCAAGGAAA ATGGAGAGAT CCCATCTTCT TCGGAGAGAG TGCACCACAG AAAAAGGCTC 
TTGCCCGACG TCACTCAGGA GGCCAAAGAA CGGGCAGAGG AGCGGATGAA AGCCCGGGAG 
AAGTCTGTGA AGAGCCAGGC ACTGAAAGAC GCCATAGCAC AACAGCGGCA CAAAATGAAA 
CAACTAGAAC CAGGGGCAAG ATCGGCCAAA GCTCTGCCAT CTACCGTTAT GTTCAGCGAT 
TACCCTGCAG TATCTCCTCT CCTGAGAACC CAGACCGAGA CTCCCACCCT GAAGCTGGAT 
ACCGACCAGG AGGTCTTTAT AACCCCCCCA GCAGCTCCTG ACGGCTCCGT CACTTCCTCG 
GAAGGTTCAG GCGGGAAACC CAAGAAAAGG CCGTCTATAT TCTCCCCGAG GAAGAAAGAG 
AAAAAGTCCA AGGGGGAAAG CAGTCGGGCG TCTGATAAAC AGGCGACGGA CGAGAGCACC 
AAACCCAAGT CCTTGTGGAA ATCCGTCTTC TCCGGGTATA AGAAGGAAAA GAAGAAGAAG 
CAGCAGCAGG CGGAGGACAA GTCTACTCCG AGCACTCCAT CCAGCAGCAC CACCGTGGAC 
TCTGGGAAAG CCAAGGTGGC AAATATGATC CGCGGAGCAG ACCTGTCCCT CCGACGTCAC 
CTGAGCTTCT CGGAGGACTC GGACCTGTCC AGCGACGATG TCTTGGAAAA ATCCTCCCAG 
AAATCTAAGC GAGAGCGGTT TCCTGAAGCC TCGTGCCGGG CCCCTGAATT TCACATGCAC 
AGAGATGAGT CGGTGTATGT CCCCCACGCC TTGGCATTCA GACGGTCATA CTCATCAAAG 
AAAACCTACA CGGACGAGGA GCTAAGCGCT AAGCTAACAA GGCGGGTGCA GAAAGCGGCA 
CGGAGACAGG CCAAGCAGGA GGAGCTAAAG CGGCTGCATC GGGCACAGAT CATTCAGCGC 
CAGCTGGAAC AAGTGGAGGA AAAGCAGCGG CAGCTGGAGG AGCGAGGGGT GGCTGTGGAG 
AAAGCTCTCC GGGGAGAAGC AGACTATTGG GGAGAGTCTT ATTACAGTGA CCTCATCGAT 
TTGCACCTGG GTGTTGATCC CGTTGCCGGG ACTCCGCGCC GTAGACCTCT CTCCTTCTGC 
CCCTGCTGTG CCCACGAAGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754447.1
CDS	355..6732
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898587.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898587.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG GGCTCATTTA GGAAAGAGTT CCCCCAGAAC 
CTGGGAGGGA GCGACGTCTG CTATTTCTGC CGCCGGAGGG TTTACGTCAT GGAGCGGCTC 
AGCGCCGAGG GCAAGTTCTT CCACCGGAGC TGCTTTAAGT GTGACTACTG TAGCACAACG 
CTGCGCCTCT CATGTTATGC CTATGACCTT GAGGATGGAA AATTTTACTG CAAACCTCAC 
TACTGTTACC GCCGGTCCGG ATACAGCCAG AGGAAGAGGC CGGCCATGCC TGCACTTTCG 
GGAAAAGAAG TCACAAGGAC TGTGGAAGAC ACCCCCGACG CGGAAGACCA AGGACGAGCC 
CCGCTGCTGC CCGCCCCCGC TGCTGAGAAG ACCCCAGGCC CCAGTGTGAA CGGCGTGGAA 
GATCACAGCG CCGCCAAGAG GCTGAGAGCC ACCCCAGAGC GCATTGAGCT TGAGAATTAC 
CGCGTCTCCC TGCAGAAGGA GGAGCTGGAG GAGGTGCCCG AGGAAACCCT GGCGGAGCAT 
AATCTGAGCA GCCTATTGGA TAAAGGGGAG GTGGAAGAGG TGGCCAGCAG CAGTTCCGAG 
TCCGAGATGG AGGAGGAGGA GGACGAAGAA GAAGAGGAGG AGGAGCCAGG AGAGTGCCTT 
CCCCCCTCAG ATCTGGGTGG GGTGCCGTGG AAAGAGGCCG TCAAGATCCA CGCCAAGTTA 
AAGGGTCAGG ACATAGATGT GGAGGTTGTG GGAACCCAGG GGGCTGGTGG CAGTGAAGAG 
GAGGAGGAGG AGGAGGAAGA AGAGGACGAA GAGGAGGAAG ATGAAGAGGT ATCAAGTGAA 
GAGGGTGAGT ACTGCCCATG GGAGAGAGAG CTTCAGCAGG GCCTGTGGAT ACAGCGTATC 
TCTGATGAAG ATACAGTTGG TATATTTAAA GCCGGGAACC TCAGACTCCA GCAAGCCCTC 
TCCCCTGTTG ACCCCACGGA AATAAAGGCA GACGTGCGCT GGACACACGT CCGCGAGGTT 
AAGGAAGAGC GGAGAGGGTC TAAGGAAGAG AGGGGAGAAG TGAGCATGTC CTCTCCTTCC 
AAAGCGCGTC ATCTCCATTC CCACCGTCAC GACCCAGTGA GAGCGTGGCT GGAGTCCGTA 
TCAGGAGCTA CTGGCGAGTA TGAAGAAGGT GAGGAGGAGG AGCTGGGTAC TTGCCTGGCG 
CAGGCTGAAG CAGCTGGTGA TGGGGACACC TCCGCAGACC TTGGTGATGA TGATGACATC 
CCGTCCGACG CCGAGGCCGA AGAACGGCTC CAGCAGTCTC AGAGGTTTAA ATTGAAGCTG 
GATGTAGCCC AAGAGCCTAA GGTGGAGCAA CCCGTCCTGC CAGCAATTGC AGATTCTGAG 
AGCGTCCCCA GCCCAGTCTC TCTGGCACCG AAAGCCCCCG TGCTGTCTCC AAGAAGGACA 
CTCAAAGCGG AAGCAGAAAA GGTTCCTGTG ATGTCTCCGA AGGCAGTGAC CTCTCCTGGG 
GGTCGGTTCT TTGCGGATCC GTATCTGCCA TTGGACAGCA GCAACAAAGC CTTAGTGGAA 
GCAAAAACTC CAACTCCTCT ATCTCCTGTT TGCTCCCAAC CAGTCCCATC GCAAGAACCT 
GTTATCCCTA CATCTCCTGT GAAGTCTCCC TCAGTCCCAG CAAACACGCG CCCACTTATA 
TCCCCATCAG CTCCTATTTG TTCCCAGCCA CTGCCGTCCT CAGAGGGTAC CCTAAATGCT 
ACAGACAGGC CCCCCGTGCG TCAGCAGCCA TCCCCTCTAA TCCCCTCTAC TTCTTTAACC 
AAACTGCCTT TTACTGGTCA CGTCATGGGA TCTGAACAAT TAGTTAGCCC TAATGCAAAA 
GAGGTCCTGA AACGTATTGA CCTTATCGAA GAGTTCTGGA CAAAAAGCGC CGAGATCAGG 
CGCAGCTTAG GGCTGGCCCC CGTGGACAGA GATCGGGTAC ATGAGATCAG TTTCCCTGCC 
CTAGCTTCAA CTCCTCTTCA GCCTCAGGCA CCTAAATCCA AACCTGAGGA GCAAAAGTCA 
CCGTCTCTGA AGCCACAACA AGTTGCAAGA AGAGTGAACC CTTCCCGGCT GGACATTGAA 
CTGCTCTCCC CTCTGACTCC AATATCCCCT TCTGAGAAGG AGCTGAAGAG CTCAGATGGT 
GAGAAGAAGG ATATCTCCAC CAGCTCTGGC TTAGGCCTGA ATGGCAGTTC CATAACACGT 
ACTGCCGCCA GTGAAAGCTT TAACACCTCT GATTCAGCCA TGCTTACTCC ACCATCCAGC 
CCTCCACCAC CTCCTCCCCA AGGGGAAGAG CCAGCTACTC TACGTAGAAG CCGTACTCCT 
TGCCAGAATG GTCCAGAATC TATCCCAGCC CCTGCCCTAG CACAGAGCAA GCCCCCTGAG 
CCCAAAAAGT CCCCCCGCTG GGAAGAATCT GTACGTAAGT CATTTGTAGA AAGTGTAGAC 
GAGATCCCCT TTGCCGATGA TGTGGAGGAC ACGTACGACG AAAGAACGGA GAATTCCAGC 
CTTCAAGAGA GGTTCTTTAC CCCACCAACG AGTCGGGTTA AACCACATAG AATTGGTTTC 
TCCAAGGAAA ATGGAGAGAT CCCATCTTCT TCGGAGAGAG TGCACCACAG AAAAAGGCTC 
TTGCCCGACG TCACTCAGGA GGCCAAAGAA CGGGCAGAGG AGCGGATGAA AGCCCGGGAG 
AAGTCTGTGA AGAGCCAGGC ACTGAAAGAC GCCATAGCAC AACAGCGGCA CAAAATGAAA 
CAACTAGAAC CAGGGGCAAG ATCGGCCAAA GCTCTGCCAT CTACCGTTAT GTTCAGCGAT 
TACCCTGCAG TATCTCCTCT CCTGAGAACC CAGACCGAGA CTCCCACCCT GAAGCTGGAT 
ACCGACCAGG AGGTCTTTAT AACCCCCCCA GCAGCTCCTG ACGGCTCCGT CACTTCCTCG 
GAAGGTTCAG GCGGGAAACC CAAGAAAAGG CCGTCTATAT TCTCCCCGAG GAAGAAAGAG 
AAAAAGTCCA AGGGGGAAAG CAGTCGGGCG TCTGATAAAC AGGCGACGGA CGAGAGCACC 
AAACCCAAGT CCTTGTGGAA ATCCGTCTTC TCCGGGTATA AGAAGGAAAA GAAGAAGAAG 
CAGCAGCAGG CGGAGGACAA GTCTACTCCG AGCACTCCAT CCAGCAGCAC CACCGTGGAC 
TCTGGGAAAG CCAAGGTGGC AAATATGATC CGCGGAGCAG ACCTGTCCCT CCGACGTCAC 
CTGAGCTTCT CGGAGGACTC GGACCTGTCC AGCGACGATG TCTTGGAAAA ATCCTCCCAG 
AAATCTAAGC GAGAGCGGTT TCCTGAAGCC TCGTGCCGGG CCCCTGAATT TCACATGCAC 
AGAGATGAGT CGGTGTATGT CCCCCACGCC TTGGCATTCA GACGGTCATA CTCATCAAAG 
AAAACCTACA CGGACGAGGA GCTAAGCGCT AAGCTAACAA GGCGGGTGCA GAAAGCGGCA 
CGGAGACAGG CCAAGCAGGA GGAGCTAAAG CGGCTGCATC GGGCACAGAT CATTCAGCGC 
CAGCTGGAAC AAGTGGAGGA AAAGCAGCGG CAGCTGGAGG AGCGAGGGGT GGCTGTGGAG 
AAAGCTCTCC GGGGAGAAGC AGACTATTGG GGAGAGTCTT ATTACAGTGA CCTCATCGAT 
TTGCACCTGG GTGTTGATCC CGTTGCCGGG ACTCCGCGCC GTAGACCTCT CTCCTTCTGC 
CCCTGCTGTG CCCACGAAGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898588.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754448.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X21, mRNA.

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CloneID	OXa54224
Clone ID Related Accession (Same CDS sequence)	XM_031898588.1
Accession Version	XM_031898588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGAGGG TGAGTACTGC 
CCATGGGAGA GAGAGCTTCA GCAGGGCCTG TGGATACAGC GTATCTCTGA TGAAGATACA 
GTTGGTATAT TTAAAGCCGG GAACCTCAGA CTCCAGCAAG CCCTCTCCCC TGTTGACCCC 
ACGGAAATAA AGGCAGACGT GCGCTGGACA CACGTCCGCG AGGTTAAGGA AGAGCGGAGA 
GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC ATGTCCTCTC CTTCCAAAGC GCGTCATCTC 
CATTCCCACC GTCACGACCC AGTGAGAGCG TGGCTGGAGT CCGTATCAGG AGCTACTGGC 
GAGTATGAAG AAGGTGAGGA GGAGGAGCTG GGTACTTGCC TGGCGCAGGC TGAAGCAGCT 
GGTGATGGGG ACACCTCCGC AGACCTTGGT GATGATGATG ACATCCCGTC CGACGCCGAG 
GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG TTTAAATTGA AGCTGGATGT AGCCCAAGAG 
CCTAAGGTGG AGCAACCCGT CCTGCCAGCA ATTGCAGATT CTGAGAGCGT CCCCAGCCCA 
GTCTCTCTGG CACCGAAAGC CCCCGTGCTG TCTCCAAGAA GGACACTCAA AGCGGAAGCA 
GAAAAGGTTC CTGTGATGTC TCCGAAGGCA GTGACCTCTC CTGGGGGTCG GTTCTTTGCG 
GATCCGTATC TGCCATTGGA CAGCAGCAAC AAAGCCTTAG TGGAAGCAAA AACTCCAACT 
CCTCTATCTC CTGTTTGCTC CCAACCAGTC CCATCGCAAG AACCTGTTAT CCCTACATCT 
CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC ACGCGCCCAC TTATATCCCC ATCAGCTCCT 
ATTTGTTCCC AGCCACTGCC GTCCTCAGAG GGTACCCTAA ATGCTACAGA CAGGCCCCCC 
GTGCGTCAGC AGCCATCCCC TCTAATCCCC TCTACTTCTT TAACCAAACT GCCTTTTACT 
GGTCACGTCA TGGGATCTGA ACAATTAGTT AGCCCTAATG CAAAAGAGGT CCTGAAACGT 
ATTGACCTTA TCGAAGAGTT CTGGACAAAA AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG 
GCCCCCGTGG ACAGAGATCG GGTACATGAG ATCAGTTTCC CTGCCCTAGC TTCAACTCCT 
CTTCAGCCTC AGGCACCTAA ATCCAAACCT GAGGAGCAAA AGTCACCGTC TCTGAAGCCA 
CAACAAGTTG CAAGAAGAGT GAACCCTTCC CGGCTGGACA TTGAACTGCT CTCCCCTCTG 
ACTCCAATAT CCCCTTCTGA GAAGGAGCTG AAGAGCTCAG ATGGTGAGAA GAAGGATATC 
TCCACCAGCT CTGGCTTAGG CCTGAATGGC AGTTCCATAA CACGTACTGC CGCCAGTGAA 
AGCTTTAACA CCTCTGATTC AGCCATGCTT ACTCCACCAT CCAGCCCTCC ACCACCTCCT 
CCCCAAGGGG AAGAGCCAGC TACTCTACGT AGAAGCCGTA CTCCTTGCCA GAATGGTCCA 
GAATCTATCC CAGCCCCTGC CCTAGCACAG AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC 
CGCTGGGAAG AATCTGTACG TAAGTCATTT GTAGAAAGTG TAGACGAGAT CCCCTTTGCC 
GATGATGTGG AGGACACGTA CGACGAAAGA ACGGAGAATT CCAGCCTTCA AGAGAGGTTC 
TTTACCCCAC CAACGAGTCG GGTTAAACCA CATAGAATTG GTTTCTCCAA GGAAAATGGA 
GAGATCCCAT CTTCTTCGGA GAGAGTGCAC CACAGAAAAA GGCTCTTGCC CGACGTCACT 
CAGGAGGCCA AAGAACGGGC AGAGGAGCGG ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC 
CAGGCACTGA AAGACGCCAT AGCACAACAG CGGCACAAAA TGAAACAACT AGAACCAGGG 
GCAAGATCGG CCAAAGCTCT GCCATCTACC GTTATGTTCA GCGATTACCC TGCAGTATCT 
CCTCTCCTGA GAACCCAGAC CGAGACTCCC ACCCTGAAGC TGGATACCGA CCAGGAGGTC 
TTTATAACCC CCCCAGCAGC TCCTGACGGC TCCGTCACTT CCTCGGAAGG TTCAGGCGGG 
AAACCCAAGA AAAGGCCGTC TATATTCTCC CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG 
GAAAGCAGTC GGGCGTCTGA TAAACAGGCG ACGGACGAGA GCACCAAACC CAAGTCCTTG 
TGGAAATCCG TCTTCTCCGG GTATAAGAAG GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG 
GACAAGTCTA CTCCGAGCAC TCCATCCAGC AGCACCACCG TGGACTCTGG GAAAGCCAAG 
GTGGCAAATA TGATCCGCGG AGCAGACCTG TCCCTCCGAC GTCACCTGAG CTTCTCGGAG 
GACTCGGACC TGTCCAGCGA CGATGTCTTG GAAAAATCCT CCCAGAAATC TAAGCGAGAG 
CGGTTTCCTG AAGCCTCGTG CCGGGCCCCT GAATTTCACA TGCACAGAGA TGAGTCGGTG 
TATGTCCCCC ACGCCTTGGC ATTCAGACGG TCATACTCAT CAAAGAAAAC CTACACGGAC 
GAGGAGCTAA GCGCTAAGCT AACAAGGCGG GTGCAGAAAG CGGCACGGAG ACAGGCCAAG 
CAGGAGGAGC TAAAGCGGCT GCATCGGGCA CAGATCATTC AGCGCCAGCT GGAACAAGTG 
GAGGAAAAGC AGCGGCAGCT GGAGGAGCGA GGGGTGGCTG TGGAGAAAGC TCTCCGGGGA 
GAAGCAGACT ATTGGGGAGA GTCTTATTAC AGTGACCTCA TCGATTTGCA CCTGGGTGTT 
GATCCCGTTG CCGGGACTCC GCGCCGTAGA CCTCTCTCCT TCTGCCCCTG CTGTGCCCAC 
GAAGGGATGG GAAAGAAGGA CGACCCGAAA CTCATGCAGG AATGGTTCCG CCTGGTGCAG 
GAGAAGAACG CGCTGGTGCG ATACGAATCG GAGCTGATGA TATTTGCCCG CGAGTTGGAG 
TTGGAGGACA GACAGAGCCG ACTTCAGCAG GAGCTGCGGG AGCGAATGGC AGTGGAAGAT 
CAATTGAAGA CGGAGCAGGA GCTGACGGAC GAGAAGAAGA TCCTGAACGA GATGCTGGAC 
GTGGTGGAAC AGAGAGATGC TCTGGTGGCC CTGTTGGAGG AGCAGAGACT GCGGGAGAAA 
GAGGAGGACA AAGATCTGGA GGAGGTCATG TTATCCAAGG GGTTTAACCT CAACTGGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754448.1
CDS	353..6655
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898588.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898588.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGAGGG TGAGTACTGC 
CCATGGGAGA GAGAGCTTCA GCAGGGCCTG TGGATACAGC GTATCTCTGA TGAAGATACA 
GTTGGTATAT TTAAAGCCGG GAACCTCAGA CTCCAGCAAG CCCTCTCCCC TGTTGACCCC 
ACGGAAATAA AGGCAGACGT GCGCTGGACA CACGTCCGCG AGGTTAAGGA AGAGCGGAGA 
GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC ATGTCCTCTC CTTCCAAAGC GCGTCATCTC 
CATTCCCACC GTCACGACCC AGTGAGAGCG TGGCTGGAGT CCGTATCAGG AGCTACTGGC 
GAGTATGAAG AAGGTGAGGA GGAGGAGCTG GGTACTTGCC TGGCGCAGGC TGAAGCAGCT 
GGTGATGGGG ACACCTCCGC AGACCTTGGT GATGATGATG ACATCCCGTC CGACGCCGAG 
GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG TTTAAATTGA AGCTGGATGT AGCCCAAGAG 
CCTAAGGTGG AGCAACCCGT CCTGCCAGCA ATTGCAGATT CTGAGAGCGT CCCCAGCCCA 
GTCTCTCTGG CACCGAAAGC CCCCGTGCTG TCTCCAAGAA GGACACTCAA AGCGGAAGCA 
GAAAAGGTTC CTGTGATGTC TCCGAAGGCA GTGACCTCTC CTGGGGGTCG GTTCTTTGCG 
GATCCGTATC TGCCATTGGA CAGCAGCAAC AAAGCCTTAG TGGAAGCAAA AACTCCAACT 
CCTCTATCTC CTGTTTGCTC CCAACCAGTC CCATCGCAAG AACCTGTTAT CCCTACATCT 
CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC ACGCGCCCAC TTATATCCCC ATCAGCTCCT 
ATTTGTTCCC AGCCACTGCC GTCCTCAGAG GGTACCCTAA ATGCTACAGA CAGGCCCCCC 
GTGCGTCAGC AGCCATCCCC TCTAATCCCC TCTACTTCTT TAACCAAACT GCCTTTTACT 
GGTCACGTCA TGGGATCTGA ACAATTAGTT AGCCCTAATG CAAAAGAGGT CCTGAAACGT 
ATTGACCTTA TCGAAGAGTT CTGGACAAAA AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG 
GCCCCCGTGG ACAGAGATCG GGTACATGAG ATCAGTTTCC CTGCCCTAGC TTCAACTCCT 
CTTCAGCCTC AGGCACCTAA ATCCAAACCT GAGGAGCAAA AGTCACCGTC TCTGAAGCCA 
CAACAAGTTG CAAGAAGAGT GAACCCTTCC CGGCTGGACA TTGAACTGCT CTCCCCTCTG 
ACTCCAATAT CCCCTTCTGA GAAGGAGCTG AAGAGCTCAG ATGGTGAGAA GAAGGATATC 
TCCACCAGCT CTGGCTTAGG CCTGAATGGC AGTTCCATAA CACGTACTGC CGCCAGTGAA 
AGCTTTAACA CCTCTGATTC AGCCATGCTT ACTCCACCAT CCAGCCCTCC ACCACCTCCT 
CCCCAAGGGG AAGAGCCAGC TACTCTACGT AGAAGCCGTA CTCCTTGCCA GAATGGTCCA 
GAATCTATCC CAGCCCCTGC CCTAGCACAG AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC 
CGCTGGGAAG AATCTGTACG TAAGTCATTT GTAGAAAGTG TAGACGAGAT CCCCTTTGCC 
GATGATGTGG AGGACACGTA CGACGAAAGA ACGGAGAATT CCAGCCTTCA AGAGAGGTTC 
TTTACCCCAC CAACGAGTCG GGTTAAACCA CATAGAATTG GTTTCTCCAA GGAAAATGGA 
GAGATCCCAT CTTCTTCGGA GAGAGTGCAC CACAGAAAAA GGCTCTTGCC CGACGTCACT 
CAGGAGGCCA AAGAACGGGC AGAGGAGCGG ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC 
CAGGCACTGA AAGACGCCAT AGCACAACAG CGGCACAAAA TGAAACAACT AGAACCAGGG 
GCAAGATCGG CCAAAGCTCT GCCATCTACC GTTATGTTCA GCGATTACCC TGCAGTATCT 
CCTCTCCTGA GAACCCAGAC CGAGACTCCC ACCCTGAAGC TGGATACCGA CCAGGAGGTC 
TTTATAACCC CCCCAGCAGC TCCTGACGGC TCCGTCACTT CCTCGGAAGG TTCAGGCGGG 
AAACCCAAGA AAAGGCCGTC TATATTCTCC CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG 
GAAAGCAGTC GGGCGTCTGA TAAACAGGCG ACGGACGAGA GCACCAAACC CAAGTCCTTG 
TGGAAATCCG TCTTCTCCGG GTATAAGAAG GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG 
GACAAGTCTA CTCCGAGCAC TCCATCCAGC AGCACCACCG TGGACTCTGG GAAAGCCAAG 
GTGGCAAATA TGATCCGCGG AGCAGACCTG TCCCTCCGAC GTCACCTGAG CTTCTCGGAG 
GACTCGGACC TGTCCAGCGA CGATGTCTTG GAAAAATCCT CCCAGAAATC TAAGCGAGAG 
CGGTTTCCTG AAGCCTCGTG CCGGGCCCCT GAATTTCACA TGCACAGAGA TGAGTCGGTG 
TATGTCCCCC ACGCCTTGGC ATTCAGACGG TCATACTCAT CAAAGAAAAC CTACACGGAC 
GAGGAGCTAA GCGCTAAGCT AACAAGGCGG GTGCAGAAAG CGGCACGGAG ACAGGCCAAG 
CAGGAGGAGC TAAAGCGGCT GCATCGGGCA CAGATCATTC AGCGCCAGCT GGAACAAGTG 
GAGGAAAAGC AGCGGCAGCT GGAGGAGCGA GGGGTGGCTG TGGAGAAAGC TCTCCGGGGA 
GAAGCAGACT ATTGGGGAGA GTCTTATTAC AGTGACCTCA TCGATTTGCA CCTGGGTGTT 
GATCCCGTTG CCGGGACTCC GCGCCGTAGA CCTCTCTCCT TCTGCCCCTG CTGTGCCCAC 
GAAGGGATGG GAAAGAAGGA CGACCCGAAA CTCATGCAGG AATGGTTCCG CCTGGTGCAG 
GAGAAGAACG CGCTGGTGCG ATACGAATCG GAGCTGATGA TATTTGCCCG CGAGTTGGAG 
TTGGAGGACA GACAGAGCCG ACTTCAGCAG GAGCTGCGGG AGCGAATGGC AGTGGAAGAT 
CAATTGAAGA CGGAGCAGGA GCTGACGGAC GAGAAGAAGA TCCTGAACGA GATGCTGGAC 
GTGGTGGAAC AGAGAGATGC TCTGGTGGCC CTGTTGGAGG AGCAGAGACT GCGGGAGAAA 
GAGGAGGACA AAGATCTGGA GGAGGTCATG TTATCCAAGG GGTTTAACCT CAACTGGTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898589.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754449.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X22, mRNA.

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CloneID	OXa54225
Clone ID Related Accession (Same CDS sequence)	XM_031898589.1
Accession Version	XM_031898589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGCCGG GAACCTCAGA 
CTCCAGCAAG CCCTCTCCCC TGTTGACCCC ACGGAAATAA AGGCAGACGT GCGCTGGACA 
CACGTCCGCG AGGTTAAGGA AGAGCGGAGA GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC 
ATGTCCTCTC CTTCCAAAGC GCGTCATCTC CATTCCCACC GTCACGACCC AGTGAGAGCG 
TGGCTGGAGT CCGTATCAGG AGCTACTGGC GAGTATGAAG AAGGTGAGGA GGAGGAGCTG 
GGTACTTGCC TGGCGCAGGC TGAAGCAGCT GGTGATGGGG ACACCTCCGC AGACCTTGGT 
GATGATGATG ACATCCCGTC CGACGCCGAG GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG 
TTTAAATTGA AGCTGGATGT AGCCCAAGAG CCTAAGGTGG AGCAACCCGT CCTGCCAGCA 
ATTGCAGATT CTGAGAGCGT CCCCAGCCCA GTCTCTCTGG CACCGAAAGC CCCCGTGCTG 
TCTCCAAGAA GGACACTCAA AGCGGAAGCA GAAAAGGTTC CTGTGATGTC TCCGAAGGCA 
GTGACCTCTC CTGGGGGTCG GTTCTTTGCG GATCCGTATC TGCCATTGGA CAGCAGCAAC 
AAAGCCTTAG TGGAAGCAAA AACTCCAACT CCTCTATCTC CTGTTTGCTC CCAACCAGTC 
CCATCGCAAG AACCTGTTAT CCCTACATCT CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC 
ACGCGCCCAC TTATATCCCC ATCAGCTCCT ATTTGTTCCC AGCCACTGCC GTCCTCAGAG 
GGTACCCTAA ATGCTACAGA CAGGCCCCCC GTGCGTCAGC AGCCATCCCC TCTAATCCCC 
TCTACTTCTT TAACCAAACT GCCTTTTACT GGTCACGTCA TGGGATCTGA ACAATTAGTT 
AGCCCTAATG CAAAAGAGGT CCTGAAACGT ATTGACCTTA TCGAAGAGTT CTGGACAAAA 
AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG GCCCCCGTGG ACAGAGATCG GGTACATGAG 
ATCAGTTTCC CTGCCCTAGC TTCAACTCCT CTTCAGCCTC AGGCACCTAA ATCCAAACCT 
GAGGAGCAAA AGTCACCGTC TCTGAAGCCA CAACAAGTTG CAAGAAGAGT GAACCCTTCC 
CGGCTGGACA TTGAACTGCT CTCCCCTCTG ACTCCAATAT CCCCTTCTGA GAAGGAGCTG 
AAGAGCTCAG ATGGTGAGAA GAAGGATATC TCCACCAGCT CTGGCTTAGG CCTGAATGGC 
AGTTCCATAA CACGTACTGC CGCCAGTGAA AGCTTTAACA CCTCTGATTC AGCCATGCTT 
ACTCCACCAT CCAGCCCTCC ACCACCTCCT CCCCAAGGGG AAGAGCCAGC TACTCTACGT 
AGAAGCCGTA CTCCTTGCCA GAATGGTCCA GAATCTATCC CAGCCCCTGC CCTAGCACAG 
AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC CGCTGGGAAG AATCTGTACG TAAGTCATTT 
GTAGAAAGTG TAGACGAGAT CCCCTTTGCC GATGATGTGG AGGACACGTA CGACGAAAGA 
ACGGAGAATT CCAGCCTTCA AGAGAGGTTC TTTACCCCAC CAACGAGTCG GGTTAAACCA 
CATAGAATTG GTTTCTCCAA GGAAAATGGA GAGATCCCAT CTTCTTCGGA GAGAGTGCAC 
CACAGAAAAA GGCTCTTGCC CGACGTCACT CAGGAGGCCA AAGAACGGGC AGAGGAGCGG 
ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC CAGGCACTGA AAGACGCCAT AGCACAACAG 
CGGCACAAAA TGAAACAACT AGAACCAGGG GCAAGATCGG CCAAAGCTCT GCCATCTACC 
GTTATGTTCA GCGATTACCC TGCAGTATCT CCTCTCCTGA GAACCCAGAC CGAGACTCCC 
ACCCTGAAGC TGGATACCGA CCAGGAGGTC TTTATAACCC CCCCAGCAGC TCCTGACGGC 
TCCGTCACTT CCTCGGAAGG TTCAGGCGGG AAACCCAAGA AAAGGCCGTC TATATTCTCC 
CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG GAAAGCAGTC GGGCGTCTGA TAAACAGGCG 
ACGGACGAGA GCACCAAACC CAAGTCCTTG TGGAAATCCG TCTTCTCCGG GTATAAGAAG 
GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG GACAAGTCTA CTCCGAGCAC TCCATCCAGC 
AGCACCACCG TGGACTCTGG GAAAGCCAAG GTGGCAAATA TGATCCGCGG AGCAGACCTG 
TCCCTCCGAC GTCACCTGAG CTTCTCGGAG GACTCGGACC TGTCCAGCGA CGATGTCTTG 
GAAAAATCCT CCCAGAAATC TAAGCGAGAG CGGTTTCCTG AAGCCTCGTG CCGGGCCCCT 
GAATTTCACA TGCACAGAGA TGAGTCGGTG TATGTCCCCC ACGCCTTGGC ATTCAGACGG 
TCATACTCAT CAAAGAAAAC CTACACGGAC GAGGAGCTAA GCGCTAAGCT AACAAGGCGG 
GTGCAGAAAG CGGCACGGAG ACAGGCCAAG CAGGAGGAGC TAAAGCGGCT GCATCGGGCA 
CAGATCATTC AGCGCCAGCT GGAACAAGTG GAGGAAAAGC AGCGGCAGCT GGAGGAGCGA 
GGGGTGGCTG TGGAGAAAGC TCTCCGGGGA GAAGCAGACT ATTGGGGAGA GTCTTATTAC 
AGTGACCTCA TCGATTTGCA CCTGGGTGTT GATCCCGTTG CCGGGACTCC GCGCCGTAGA 
CCTCTCTCCT TCTGCCCCTG CTGTGCCCAC GAAGGGATGG GAAAGAAGGA CGACCCGAAA 
CTCATGCAGG AATGGTTCCG CCTGGTGCAG GAGAAGAACG CGCTGGTGCG ATACGAATCG 
GAGCTGATGA TATTTGCCCG CGAGTTGGAG TTGGAGGACA GACAGAGCCG ACTTCAGCAG 
GAGCTGCGGG AGCGAATGGC AGTGGAAGAT CAATTGAAGA CGGAGCAGGA GCTGACGGAC 
GAGAAGAAGA TCCTGAACGA GATGCTGGAC GTGGTGGAAC AGAGAGATGC TCTGGTGGCC 
CTGTTGGAGG AGCAGAGACT GCGGGAGAAA GAGGAGGACA AAGATCTGGA GGAGGTCATG 
TTATCCAAGG GGTTTAACCT CAACTGGTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754449.1
CDS	352..6564
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898589.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898589.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGCCGG GAACCTCAGA 
CTCCAGCAAG CCCTCTCCCC TGTTGACCCC ACGGAAATAA AGGCAGACGT GCGCTGGACA 
CACGTCCGCG AGGTTAAGGA AGAGCGGAGA GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC 
ATGTCCTCTC CTTCCAAAGC GCGTCATCTC CATTCCCACC GTCACGACCC AGTGAGAGCG 
TGGCTGGAGT CCGTATCAGG AGCTACTGGC GAGTATGAAG AAGGTGAGGA GGAGGAGCTG 
GGTACTTGCC TGGCGCAGGC TGAAGCAGCT GGTGATGGGG ACACCTCCGC AGACCTTGGT 
GATGATGATG ACATCCCGTC CGACGCCGAG GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG 
TTTAAATTGA AGCTGGATGT AGCCCAAGAG CCTAAGGTGG AGCAACCCGT CCTGCCAGCA 
ATTGCAGATT CTGAGAGCGT CCCCAGCCCA GTCTCTCTGG CACCGAAAGC CCCCGTGCTG 
TCTCCAAGAA GGACACTCAA AGCGGAAGCA GAAAAGGTTC CTGTGATGTC TCCGAAGGCA 
GTGACCTCTC CTGGGGGTCG GTTCTTTGCG GATCCGTATC TGCCATTGGA CAGCAGCAAC 
AAAGCCTTAG TGGAAGCAAA AACTCCAACT CCTCTATCTC CTGTTTGCTC CCAACCAGTC 
CCATCGCAAG AACCTGTTAT CCCTACATCT CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC 
ACGCGCCCAC TTATATCCCC ATCAGCTCCT ATTTGTTCCC AGCCACTGCC GTCCTCAGAG 
GGTACCCTAA ATGCTACAGA CAGGCCCCCC GTGCGTCAGC AGCCATCCCC TCTAATCCCC 
TCTACTTCTT TAACCAAACT GCCTTTTACT GGTCACGTCA TGGGATCTGA ACAATTAGTT 
AGCCCTAATG CAAAAGAGGT CCTGAAACGT ATTGACCTTA TCGAAGAGTT CTGGACAAAA 
AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG GCCCCCGTGG ACAGAGATCG GGTACATGAG 
ATCAGTTTCC CTGCCCTAGC TTCAACTCCT CTTCAGCCTC AGGCACCTAA ATCCAAACCT 
GAGGAGCAAA AGTCACCGTC TCTGAAGCCA CAACAAGTTG CAAGAAGAGT GAACCCTTCC 
CGGCTGGACA TTGAACTGCT CTCCCCTCTG ACTCCAATAT CCCCTTCTGA GAAGGAGCTG 
AAGAGCTCAG ATGGTGAGAA GAAGGATATC TCCACCAGCT CTGGCTTAGG CCTGAATGGC 
AGTTCCATAA CACGTACTGC CGCCAGTGAA AGCTTTAACA CCTCTGATTC AGCCATGCTT 
ACTCCACCAT CCAGCCCTCC ACCACCTCCT CCCCAAGGGG AAGAGCCAGC TACTCTACGT 
AGAAGCCGTA CTCCTTGCCA GAATGGTCCA GAATCTATCC CAGCCCCTGC CCTAGCACAG 
AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC CGCTGGGAAG AATCTGTACG TAAGTCATTT 
GTAGAAAGTG TAGACGAGAT CCCCTTTGCC GATGATGTGG AGGACACGTA CGACGAAAGA 
ACGGAGAATT CCAGCCTTCA AGAGAGGTTC TTTACCCCAC CAACGAGTCG GGTTAAACCA 
CATAGAATTG GTTTCTCCAA GGAAAATGGA GAGATCCCAT CTTCTTCGGA GAGAGTGCAC 
CACAGAAAAA GGCTCTTGCC CGACGTCACT CAGGAGGCCA AAGAACGGGC AGAGGAGCGG 
ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC CAGGCACTGA AAGACGCCAT AGCACAACAG 
CGGCACAAAA TGAAACAACT AGAACCAGGG GCAAGATCGG CCAAAGCTCT GCCATCTACC 
GTTATGTTCA GCGATTACCC TGCAGTATCT CCTCTCCTGA GAACCCAGAC CGAGACTCCC 
ACCCTGAAGC TGGATACCGA CCAGGAGGTC TTTATAACCC CCCCAGCAGC TCCTGACGGC 
TCCGTCACTT CCTCGGAAGG TTCAGGCGGG AAACCCAAGA AAAGGCCGTC TATATTCTCC 
CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG GAAAGCAGTC GGGCGTCTGA TAAACAGGCG 
ACGGACGAGA GCACCAAACC CAAGTCCTTG TGGAAATCCG TCTTCTCCGG GTATAAGAAG 
GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG GACAAGTCTA CTCCGAGCAC TCCATCCAGC 
AGCACCACCG TGGACTCTGG GAAAGCCAAG GTGGCAAATA TGATCCGCGG AGCAGACCTG 
TCCCTCCGAC GTCACCTGAG CTTCTCGGAG GACTCGGACC TGTCCAGCGA CGATGTCTTG 
GAAAAATCCT CCCAGAAATC TAAGCGAGAG CGGTTTCCTG AAGCCTCGTG CCGGGCCCCT 
GAATTTCACA TGCACAGAGA TGAGTCGGTG TATGTCCCCC ACGCCTTGGC ATTCAGACGG 
TCATACTCAT CAAAGAAAAC CTACACGGAC GAGGAGCTAA GCGCTAAGCT AACAAGGCGG 
GTGCAGAAAG CGGCACGGAG ACAGGCCAAG CAGGAGGAGC TAAAGCGGCT GCATCGGGCA 
CAGATCATTC AGCGCCAGCT GGAACAAGTG GAGGAAAAGC AGCGGCAGCT GGAGGAGCGA 
GGGGTGGCTG TGGAGAAAGC TCTCCGGGGA GAAGCAGACT ATTGGGGAGA GTCTTATTAC 
AGTGACCTCA TCGATTTGCA CCTGGGTGTT GATCCCGTTG CCGGGACTCC GCGCCGTAGA 
CCTCTCTCCT TCTGCCCCTG CTGTGCCCAC GAAGGGATGG GAAAGAAGGA CGACCCGAAA 
CTCATGCAGG AATGGTTCCG CCTGGTGCAG GAGAAGAACG CGCTGGTGCG ATACGAATCG 
GAGCTGATGA TATTTGCCCG CGAGTTGGAG TTGGAGGACA GACAGAGCCG ACTTCAGCAG 
GAGCTGCGGG AGCGAATGGC AGTGGAAGAT CAATTGAAGA CGGAGCAGGA GCTGACGGAC 
GAGAAGAAGA TCCTGAACGA GATGCTGGAC GTGGTGGAAC AGAGAGATGC TCTGGTGGCC 
CTGTTGGAGG AGCAGAGACT GCGGGAGAAA GAGGAGGACA AAGATCTGGA GGAGGTCATG 
TTATCCAAGG GGTTTAACCT CAACTGGTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_031898590.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754450.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X24, mRNA.

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CloneID	OXa54227
Clone ID Related Accession (Same CDS sequence)	XM_031898590.1
Accession Version	XM_031898590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	F-actin-monooxygenase MICAL3 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGCCGG GAACCTCAGA 
CTCCAGCAAG CCCTCTCCCC TGTTGACCCC ACGGAAATAA AGGCAGACGT GCGCTGGACA 
CACGTCCGCG AGGTTAAGGA AGAGCGGAGA GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC 
ATGTCCTCTC CTTCCAAAGC GCGTCATCTC CATTCCCACC GTCACGACCC AGTGAGAGCG 
TGGCTGGAGT CCGTATCAGG AGCTACTGGC GAGTATGAAG AAGGTGAGGA GGAGGAGCTG 
GGTACTTGCC TGGCGCAGGC TGAAGCAGCT GGTGATGGGG ACACCTCCGC AGACCTTGGT 
GATGATGATG ACATCCCGTC CGACGCCGAG GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG 
TTTAAATTGA AGCTGGATGT AGCCCAAGAG CCTAAGGTGG AGCAACCCGT CCTGCCAGCA 
ATTGCAGATT CTGAGAGCGT CCCCAGCCCA GTCTCTCTGG CACCGAAAGC CCCCGTGCTG 
TCTCCAAGAA GGACACTCAA AGCGGAAGCA GAAAAGGTTC CTGTGATGTC TCCGAAGGCA 
GTGACCTCTC CTGGGGGTCG GTTCTTTGCG GATCCGTATC TGCCATTGGA CAGCAGCAAC 
AAAGCCTTAG TGGAAGCAAA AACTCCAACT CCTCTATCTC CTGTTTGCTC CCAACCAGTC 
CCATCGCAAG AACCTGTTAT CCCTACATCT CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC 
ACGCGCCCAC TTATATCCCC ATCAGCTCCT ATTTGTTCCC AGCCACTGCC GTCCTCAGAG 
GGTACCCTAA ATGCTACAGA CAGGCCCCCC GTGCGTCAGC AGCCATCCCC TCTAATCCCC 
TCTACTTCTT TAACCAAACT GCCTTTTACT GGTCACGTCA TGGGATCTGA ACAATTAGTT 
AGCCCTAATG CAAAAGAGGT CCTGAAACGT ATTGACCTTA TCGAAGAGTT CTGGACAAAA 
AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG GCCCCCGTGG ACAGAGATCG GGTACATGAG 
ATCAGTTTCC CTGCCCTAGC TTCAACTCCT CTTCAGCCTC AGGCACCTAA ATCCAAACCT 
GAGGAGCAAA AGTCACCGTC TCTGAAGCCA CAACAAGTTG CAAGAAGAGT GAACCCTTCC 
CGGCTGGACA TTGAACTGCT CTCCCCTCTG ACTCCAATAT CCCCTTCTGA GAAGGAGCTG 
AAGAGCTCAG ATGGTGAGAA GAAGGATATC TCCACCAGCT CTGGCTTAGG CCTGAATGGC 
AGTTCCATAA CACGTACTGC CGCCAGTGAA AGCTTTAACA CCTCTGATTC AGCCATGCTT 
ACTCCACCAT CCAGCCCTCC ACCACCTCCT CCCCAAGGGG AAGAGCCAGC TACTCTACGT 
AGAAGCCGTA CTCCTTGCCA GAATGGTCCA GAATCTATCC CAGCCCCTGC CCTAGCACAG 
AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC CGCTGGGAAG AATCTGTACG TAAGTCATTT 
GTAGAAAGTG TAGACGAGAT CCCCTTTGCC GATGATGTGG AGGACACGTA CGACGAAAGA 
ACGGAGAATT CCAGCCTTCA AGAGAGGTTC TTTACCCCAC CAACGAGTCG GGTTAAACCA 
CATAGAATTG GTTTCTCCAA GGAAAATGGA GAGATCCCAT CTTCTTCGGA GAGAGTGCAC 
CACAGAAAAA GGCTCTTGCC CGACGTCACT CAGGAGGCCA AAGAACGGGC AGAGGAGCGG 
ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC CAGGCACTGA AAGACGCCAT AGCACAACAG 
CGGCACAAAA TGAAACAACT AGAACCAGGG GCAAGATCGG CCAAAGCTCT GCCATCTACC 
GTTATGTTCA GCGATTACCC TGCAGTATCT CCTCTCCTGA GAACCCAGAC CGAGACTCCC 
ACCCTGAAGC TGGATACCGA CCAGGAGGTC TTTATAACCC CCCCAGCAGC TCCTGACGGC 
TCCGTCACTT CCTCGGAAGG TTCAGGCGGG AAACCCAAGA AAAGGCCGTC TATATTCTCC 
CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG GAAAGCAGTC GGGCGTCTGA TAAACAGGCG 
ACGGACGAGA GCACCAAACC CAAGTCCTTG TGGAAATCCG TCTTCTCCGG GTATAAGAAG 
GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG GACAAGTCTA CTCCGAGCAC TCCATCCAGC 
AGCACCACCG TGGACTCTGG GAAAGCCAAG GTGGCAAATA TGATCCGCGG AGCAGACCTG 
TCCCTCCGAC GTCACCTGAG CTTCTCGGAG GACTCGGACC TGTCCAGCGA CGATGTCTTG 
GAAAAATCCT CCCAGAAATC TAAGCGAGAG CGGTTTCCTG AAGCCTCGTG CCGGGCCCCT 
GAATTTCACA TGCACAGAGA TGAGTCGGTG TATGTCCCCC ACGCCTTGGC ATTCAGACGG 
TCATACTCAT CAAAGAAAAC CTACACGGAC GAGGAGCTAA GCGCTAAGCT AACAAGGCGG 
GTGCAGAAAG CGGCACGGAG ACAGGCCAAG CAGGAGGAGC TAAAGCGGCT GCATCGGGCA 
CAGATCATTC AGCGCCAGCT GGAACAAGTG GAGGAAAAGC AGCGGCAGCT GGAGGAGCGA 
GGGGTGGCTG TGGAGAAAGC TCTCCGGGGA GAAGCAGGGA TGGGAAAGAA GGACGACCCG 
AAACTCATGC AGGAATGGTT CCGCCTGGTG CAGGAGAAGA ACGCGCTGGT GCGATACGAA 
TCGGAGCTGA TGATATTTGC CCGCGAGTTG GAGTTGGAGG ACAGACAGAG CCGACTTCAG 
CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA GATCAATTGA AGACGGAGCA GGAGCTGACG 
GACGAGAAGA AGATCCTGAA CGAGATGCTG GACGTGGTGG AACAGAGAGA TGCTCTGGTG 
GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG AAAGAGGAGG ACAAAGATCT GGAGGAGGTC 
ATGTTATCCA AGGGGTTTAA CCTCAACTGG TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754450.1
CDS	350..6445
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEAGNLRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRERFPEASCRAPEFHMHRDESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_031898590.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898590.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC CATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GCGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGCCGG GAACCTCAGA 
CTCCAGCAAG CCCTCTCCCC TGTTGACCCC ACGGAAATAA AGGCAGACGT GCGCTGGACA 
CACGTCCGCG AGGTTAAGGA AGAGCGGAGA GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC 
ATGTCCTCTC CTTCCAAAGC GCGTCATCTC CATTCCCACC GTCACGACCC AGTGAGAGCG 
TGGCTGGAGT CCGTATCAGG AGCTACTGGC GAGTATGAAG AAGGTGAGGA GGAGGAGCTG 
GGTACTTGCC TGGCGCAGGC TGAAGCAGCT GGTGATGGGG ACACCTCCGC AGACCTTGGT 
GATGATGATG ACATCCCGTC CGACGCCGAG GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG 
TTTAAATTGA AGCTGGATGT AGCCCAAGAG CCTAAGGTGG AGCAACCCGT CCTGCCAGCA 
ATTGCAGATT CTGAGAGCGT CCCCAGCCCA GTCTCTCTGG CACCGAAAGC CCCCGTGCTG 
TCTCCAAGAA GGACACTCAA AGCGGAAGCA GAAAAGGTTC CTGTGATGTC TCCGAAGGCA 
GTGACCTCTC CTGGGGGTCG GTTCTTTGCG GATCCGTATC TGCCATTGGA CAGCAGCAAC 
AAAGCCTTAG TGGAAGCAAA AACTCCAACT CCTCTATCTC CTGTTTGCTC CCAACCAGTC 
CCATCGCAAG AACCTGTTAT CCCTACATCT CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC 
ACGCGCCCAC TTATATCCCC ATCAGCTCCT ATTTGTTCCC AGCCACTGCC GTCCTCAGAG 
GGTACCCTAA ATGCTACAGA CAGGCCCCCC GTGCGTCAGC AGCCATCCCC TCTAATCCCC 
TCTACTTCTT TAACCAAACT GCCTTTTACT GGTCACGTCA TGGGATCTGA ACAATTAGTT 
AGCCCTAATG CAAAAGAGGT CCTGAAACGT ATTGACCTTA TCGAAGAGTT CTGGACAAAA 
AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG GCCCCCGTGG ACAGAGATCG GGTACATGAG 
ATCAGTTTCC CTGCCCTAGC TTCAACTCCT CTTCAGCCTC AGGCACCTAA ATCCAAACCT 
GAGGAGCAAA AGTCACCGTC TCTGAAGCCA CAACAAGTTG CAAGAAGAGT GAACCCTTCC 
CGGCTGGACA TTGAACTGCT CTCCCCTCTG ACTCCAATAT CCCCTTCTGA GAAGGAGCTG 
AAGAGCTCAG ATGGTGAGAA GAAGGATATC TCCACCAGCT CTGGCTTAGG CCTGAATGGC 
AGTTCCATAA CACGTACTGC CGCCAGTGAA AGCTTTAACA CCTCTGATTC AGCCATGCTT 
ACTCCACCAT CCAGCCCTCC ACCACCTCCT CCCCAAGGGG AAGAGCCAGC TACTCTACGT 
AGAAGCCGTA CTCCTTGCCA GAATGGTCCA GAATCTATCC CAGCCCCTGC CCTAGCACAG 
AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC CGCTGGGAAG AATCTGTACG TAAGTCATTT 
GTAGAAAGTG TAGACGAGAT CCCCTTTGCC GATGATGTGG AGGACACGTA CGACGAAAGA 
ACGGAGAATT CCAGCCTTCA AGAGAGGTTC TTTACCCCAC CAACGAGTCG GGTTAAACCA 
CATAGAATTG GTTTCTCCAA GGAAAATGGA GAGATCCCAT CTTCTTCGGA GAGAGTGCAC 
CACAGAAAAA GGCTCTTGCC CGACGTCACT CAGGAGGCCA AAGAACGGGC AGAGGAGCGG 
ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC CAGGCACTGA AAGACGCCAT AGCACAACAG 
CGGCACAAAA TGAAACAACT AGAACCAGGG GCAAGATCGG CCAAAGCTCT GCCATCTACC 
GTTATGTTCA GCGATTACCC TGCAGTATCT CCTCTCCTGA GAACCCAGAC CGAGACTCCC 
ACCCTGAAGC TGGATACCGA CCAGGAGGTC TTTATAACCC CCCCAGCAGC TCCTGACGGC 
TCCGTCACTT CCTCGGAAGG TTCAGGCGGG AAACCCAAGA AAAGGCCGTC TATATTCTCC 
CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG GAAAGCAGTC GGGCGTCTGA TAAACAGGCG 
ACGGACGAGA GCACCAAACC CAAGTCCTTG TGGAAATCCG TCTTCTCCGG GTATAAGAAG 
GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG GACAAGTCTA CTCCGAGCAC TCCATCCAGC 
AGCACCACCG TGGACTCTGG GAAAGCCAAG GTGGCAAATA TGATCCGCGG AGCAGACCTG 
TCCCTCCGAC GTCACCTGAG CTTCTCGGAG GACTCGGACC TGTCCAGCGA CGATGTCTTG 
GAAAAATCCT CCCAGAAATC TAAGCGAGAG CGGTTTCCTG AAGCCTCGTG CCGGGCCCCT 
GAATTTCACA TGCACAGAGA TGAGTCGGTG TATGTCCCCC ACGCCTTGGC ATTCAGACGG 
TCATACTCAT CAAAGAAAAC CTACACGGAC GAGGAGCTAA GCGCTAAGCT AACAAGGCGG 
GTGCAGAAAG CGGCACGGAG ACAGGCCAAG CAGGAGGAGC TAAAGCGGCT GCATCGGGCA 
CAGATCATTC AGCGCCAGCT GGAACAAGTG GAGGAAAAGC AGCGGCAGCT GGAGGAGCGA 
GGGGTGGCTG TGGAGAAAGC TCTCCGGGGA GAAGCAGGGA TGGGAAAGAA GGACGACCCG 
AAACTCATGC AGGAATGGTT CCGCCTGGTG CAGGAGAAGA ACGCGCTGGT GCGATACGAA 
TCGGAGCTGA TGATATTTGC CCGCGAGTTG GAGTTGGAGG ACAGACAGAG CCGACTTCAG 
CAGGAGCTGC GGGAGCGAAT GGCAGTGGAA GATCAATTGA AGACGGAGCA GGAGCTGACG 
GACGAGAAGA AGATCCTGAA CGAGATGCTG GACGTGGTGG AACAGAGAGA TGCTCTGGTG 
GCCCTGTTGG AGGAGCAGAG ACTGCGGGAG AAAGAGGAGG ACAAAGATCT GGAGGAGGTC 
ATGTTATCCA AGGGGTTTAA CCTCAACTGG TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959365.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814819.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X1, mRNA.

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CloneID	OXa33553
Clone ID Related Accession (Same CDS sequence)	XM_012959365.2
Accession Version	XM_012959365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGA CTATTGGGGA GAGTCTTATT ACAGTGACCT CATCGATTTG 
CACCTGGGTG TTGATCCCGT TGCCGGGACT CCGCGCCGTA GACCTCTCTC CTTTTGCCCC 
TGCTGTGCCC ACGAAGGGAT GGGAAAGAAG GACGACCCGA AACTCATGCA GGAATGGTTC 
CGCCTGGTGC AGGAGAAGAA CGCGCTGGTG CGATACGAAT CGGAGCTGAT GATATTTGCC 
CGCGAGTTGG AGTTGGAGGA CAGACAGAGC CGACTTCAGC AGGAGCTGCG GGAGCGAATG 
GCAGTGGAAG ATCAATTGAA GACGGAGCAG GAGCTGACGG ACGAGAAGAA GATCCTGAAC 
GAGATGCTGG ACGTGGTGGA ACAGAGAGAT GCTCTGGTGG CCCTGTTGGA GGAGCAGAGA 
CTGCGGGAGA AAGAGGAGGA CAAAGATCTG GAGGAGGTCA TGTTATCCAA GGGGTTTAAC 
CTCAACTGGT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814819.1
CDS	400..7074
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959365.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959365.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGA CTATTGGGGA GAGTCTTATT ACAGTGACCT CATCGATTTG 
CACCTGGGTG TTGATCCCGT TGCCGGGACT CCGCGCCGTA GACCTCTCTC CTTTTGCCCC 
TGCTGTGCCC ACGAAGGGAT GGGAAAGAAG GACGACCCGA AACTCATGCA GGAATGGTTC 
CGCCTGGTGC AGGAGAAGAA CGCGCTGGTG CGATACGAAT CGGAGCTGAT GATATTTGCC 
CGCGAGTTGG AGTTGGAGGA CAGACAGAGC CGACTTCAGC AGGAGCTGCG GGAGCGAATG 
GCAGTGGAAG ATCAATTGAA GACGGAGCAG GAGCTGACGG ACGAGAAGAA GATCCTGAAC 
GAGATGCTGG ACGTGGTGGA ACAGAGAGAT GCTCTGGTGG CCCTGTTGGA GGAGCAGAGA 
CTGCGGGAGA AAGAGGAGGA CAAAGATCTG GAGGAGGTCA TGTTATCCAA GGGGTTTAAC 
CTCAACTGGT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959367.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814821.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X2, mRNA.

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CloneID	OXa33554
Clone ID Related Accession (Same CDS sequence)	XM_012959367.2
Accession Version	XM_012959367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959367.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGT TGATCCCGTT GCCGGGACTC CGCGCCGTAG ACCTCTCTCC 
TTTTGCCCCT GCTGTGCCCA CGAAGGGATG GGAAAGAAGG ACGACCCGAA ACTCATGCAG 
GAATGGTTCC GCCTGGTGCA GGAGAAGAAC GCGCTGGTGC GATACGAATC GGAGCTGATG 
ATATTTGCCC GCGAGTTGGA GTTGGAGGAC AGACAGAGCC GACTTCAGCA GGAGCTGCGG 
GAGCGAATGG CAGTGGAAGA TCAATTGAAG ACGGAGCAGG AGCTGACGGA CGAGAAGAAG 
ATCCTGAACG AGATGCTGGA CGTGGTGGAA CAGAGAGATG CTCTGGTGGC CCTGTTGGAG 
GAGCAGAGAC TGCGGGAGAA AGAGGAGGAC AAAGATCTGG AGGAGGTCAT GTTATCCAAG 
GGGTTTAACC TCAACTGGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814821.1
CDS	399..7022
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959367.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959367.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGT TGATCCCGTT GCCGGGACTC CGCGCCGTAG ACCTCTCTCC 
TTTTGCCCCT GCTGTGCCCA CGAAGGGATG GGAAAGAAGG ACGACCCGAA ACTCATGCAG 
GAATGGTTCC GCCTGGTGCA GGAGAAGAAC GCGCTGGTGC GATACGAATC GGAGCTGATG 
ATATTTGCCC GCGAGTTGGA GTTGGAGGAC AGACAGAGCC GACTTCAGCA GGAGCTGCGG 
GAGCGAATGG CAGTGGAAGA TCAATTGAAG ACGGAGCAGG AGCTGACGGA CGAGAAGAAG 
ATCCTGAACG AGATGCTGGA CGTGGTGGAA CAGAGAGATG CTCTGGTGGC CCTGTTGGAG 
GAGCAGAGAC TGCGGGAGAA AGAGGAGGAC AAAGATCTGG AGGAGGTCAT GTTATCCAAG 
GGGTTTAACC TCAACTGGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959371.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814825.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X3, mRNA.

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CloneID	OXa33557
Clone ID Related Accession (Same CDS sequence)	XM_012959371.2
Accession Version	XM_012959371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGA CTATTGGGGA GAGTCTTATT ACAGTGACCT CATCGATTTG 
CACCTGGGTG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814825.1
CDS	399..7007
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959371.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959371.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGA CTATTGGGGA GAGTCTTATT ACAGTGACCT CATCGATTTG 
CACCTGGGTG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959373.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814827.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X4, mRNA.

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CloneID	OXa33559
Clone ID Related Accession (Same CDS sequence)	XM_012959373.2
Accession Version	XM_012959373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG ACAACCCCGG TGCTCGGCAG TAGCCGTTGG 
GAGCAGTGTC CCACACTGGA GAGGTCCGAG ACCCAATCCC GCTCTCCGGC TGGCCAATGG 
CAGCAGACCC TTGCTCAGCG TTCCTCCGAC CGCCCCTCGT CCCCCTCGTC CGATGCCCTT 
CGACAGAAGT ATATAAAGAT GTACACAGGT GGAGTGAGCT CATTGGCTGA GCAAATAGCC 
AATCAGCTTC AAAAGAAGGA AACGCCAAAA CCTCTGTTCG GGAAGAAAGT GGGCTCATTT 
AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG AGCGACGTCT GCTATTTCTG CCGCCGGAGG 
GTTTACGTCA TGGAGCGGCT CAGCGCCGAG GGCAAGTTCT TCCACCGGAG CTGCTTTAAG 
TGTGACTACT GTAGCACAAC GCTGCGCCTC TCATGTTATG CCTATGACCT TGAGGATGGA 
AAATTTTACT GCAAACCTCA CTACTGTTAC CGCCGGTCCG GATACAGCCA GAGGAAGAGG 
CCGGCCATGC CTGCACTTTC GGGAAAAGAA GTCACAAGGA CTGTGGAAGA CACCCCCGAC 
GCGGAAGACC AAGGACGAGC CCCGCTGCTG CCCGCCCCCG CTGCTGAGAA GACCCCAGGC 
CCCAGTGTGA ACGGCGTGGA AGATCACAGC GCCGCCAAGA GGCTGAGAGC CACCCCAGAG 
CGCATTGAGC TTGAGAATTA CCGCGTCTCC CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC 
GAGGAAACCC TGGCGGAGCA TAATCTGAGC AGCCTATTGG ATAAAGGGGA GGTGGAAGAG 
GTGGCCAGCA GCAGTTCCGA GTCCGAGATG GAGGAGGAGG AGGACGAAGA AGAAGAGGAG 
GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA GATCTGGGTG GGGTGCCGTG GAAAGAGGCC 
GTCAAGATCC ACGCCAAGTT AAAGGGTCAG GACATAGATG TGGAGGTTGT GGGAACCCAG 
GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG GAGGAGGAAG AAGAGGACGA AGAGGAGGAA 
GATGAAGAGG TATCAAGTGA AGAGGGTGAG TACTGCCCAT GGGAGAGAGA GCTTCAGCAG 
GGCCTGTGGA TACAGCGTAT CTCTGATGAA GATACAGTTG GTATATTTAA AGCCGGGAAC 
TTCAGACTCC AGCAAGCCCT CTCCCCTGTT GACCCCACGG AAATAAAGGC AGACGTGCGC 
TGGACACACG TCCGCGAGGT TAAGGAAGAG CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA 
GTGAGCATGT CCTCTCCTTC CAAAGCGCGT CATCTCCATT CCCACCGTCA CGACCCAGTG 
AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG 
GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA GCAGCTGGTG ATGGGGACAC CTCCGCAGAC 
CTTGGTGATG ATGATGACAT CCCGTCCGAC GCCGAGGCCG AAGAACGGCT CCAGCAGTCT 
CAGAGGTTTA AATTGAAGCT GGATGTAGCC CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG 
CCAGCAATTG CAGATTCTGA GAGCGTCCCC AGCCCAGTCT CTCTGGCACC GAAAGCCCCC 
GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG GAAGCAGAAA AGGTTCCTGT GATGTCTCCG 
AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC TTTGCGGATC CGTATCTGCC ATTGGACAGC 
AGCAACAAAG CCTTAGTGGA AGCAAAAACT CCAACTCCTC TATCTCCTGT TTGCTCCCAA 
CCAGTCCCAT CGCAAGAACC TGTTATCCCT ACATCTCCTG TGAAGTCTCC CTCAGTCCCA 
GCAAACACGC GCCCACTTAT ATCCCCATCA GCTCCTATTT GTTCCCAGCC ACTGCCGTCC 
TCAGAGGGTA CCCTAAATGC TACAGACAGG CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA 
ATCCCCTCTA CTTCTTTAAC CAAACTGCCT TTTACTGGTC ACGTCATGGG ATCTGAACAA 
TTAGTTAGCC CTAATGCAAA AGAGGTCCTG AAACGTATTG ACCTTATCGA AGAGTTCTGG 
ACAAAAAGCG CCGAGATCAG GCGCAGCTTA GGGCTGGCCC CCGTGGACAG AGATCGGGTA 
CATGAGATCA GTTTCCCTGC CCTAGCTTCA ACTCCTCTTC AGCCTCAGGC ACCTAAATCC 
AAACCTGAGG AGCAAAAGTC ACCGTCTCTG AAGCCACAAC AAGTTGCAAG AAGAGTGAAC 
CCTTCCCGGC TGGACATTGA ACTGCTCTCC CCTCTGACTC CAATATCCCC TTCTGAGAAG 
GAGCTGAAGA GCTCAGATGG TGAGAAGAAG GATATCTCCA CCAGCTCTGG CTTAGGCCTG 
AATGGCAGTT CCATAACACG TACTGCCGCC AGTGAAAGCT TTAACACCTC TGATTCAGCC 
ATGCTTACTC CACCATCCAG CCCTCCACCA CCTCCTCCCC AAGGGGAAGA GCCAGCTACT 
CTACGTAGAA GCCGTACTCC TTGCCAGAAT GGTCCAGAAT CTATCCCAGC CCCTGCCCTA 
GCACAGAGCA AGCCCCCTGA GCCCAAAAAG TCCCCCCGCT GGGAAGAATC TGTACGTAAG 
TCATTTGTAG AAAGTGTAGA CGAGATCCCC TTTGCCGATG ATGTGGAGGA CACGTACGAC 
GAAAGAACGG AGAATTCCAG CCTTCAAGAG AGGTTCTTTA CCCCACCAAC GAGTCGGGTT 
AAACCACATA GAATTGGTTT CTCCAAGGAA AATGGAGAGA TCCCATCTTC TTCGGAGAGA 
GTGCACCACA GAAAAAGGCT CTTGCCCGAC GTCACTCAGG AGGCCAAAGA ACGGGCAGAG 
GAGCGGATGA AAGCCCGGGA GAAGTCTGTG AAGAGCCAGG CACTGAAAGA CGCCATAGCA 
CAACAGCGGC ACAAAATGAA ACAACTAGAA CCAGGGGCAA GATCGGCCAA AGCTCTGCCA 
TCTACCGTTA TGTTCAGCGA TTACCCTGCA GTATCTCCTC TCCTGAGAAC CCAGACCGAG 
ACTCCCACCC TGAAGCTGGA TACCGACCAG GAGGTCTTTA TAACCCCCCC AGCAGCTCCT 
GACGGCTCCG TCACTTCCTC GGAAGGTTCA GGCGGGAAAC CCAAGAAAAG GCCGTCTATA 
TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC AAGGGGGAAA GCAGTCGGGC GTCCGATAAA 
CAGGCGACGG ACGAGAGCAC CAAACCCAAG TCCTTGTGGA AATCCGTCTT CTCCGGGTAT 
AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG GCGGAGGACA AGTCTACTCC GAGCACTCCA 
TCCAGCAGCA CCACCGTGGA CTCTGGGAAA GCCAAGGTGG CAAATATGAT CCGCGGAGCA 
GACCTGTCCC TCCGACGTCA CCTGAGCTTC TCGGAGGACT CGGACCTGTC CAGCGACGAT 
GTCTTGGAAA AATCCTCCCA GAAATCTAAG CGAGAGTCGG TGTATGTCCC CCACGCCTTG 
GCATTCAGAC GGTCATACTC ATCAAAGAAA ACCTACACGG ACGAGGAGCT AAGCGCTAAG 
CTAACAAGGC GGGTGCAGAA AGCGGCACGG AGACAGGCCA AGCAGGAGGA GCTAAAGCGG 
CTGCATCGGG CACAGATCAT TCAGCGCCAG CTGGAACAAG TGGAGGAAAA GCAGCGGCAG 
CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA GCTCTCCGGG GAGAAGCAGA CTATTGGGGA 
GAGTCTTATT ACAGTGACCT CATCGATTTG CACCTGGGTG TTGATCCCGT TGCCGGGACT 
CCGCGCCGTA GACCTCTCTC CTTTTGCCCC TGCTGTGCCC ACGAAGGGAT GGGAAAGAAG 
GACGACCCGA AACTCATGCA GGAATGGTTC CGCCTGGTGC AGGAGAAGAA CGCGCTGGTG 
CGATACGAAT CGGAGCTGAT GATATTTGCC CGCGAGTTGG AGTTGGAGGA CAGACAGAGC 
CGACTTCAGC AGGAGCTGCG GGAGCGAATG GCAGTGGAAG ATCAATTGAA GACGGAGCAG 
GAGCTGACGG ACGAGAAGAA GATCCTGAAC GAGATGCTGG ACGTGGTGGA ACAGAGAGAT 
GCTCTGGTGG CCCTGTTGGA GGAGCAGAGA CTGCGGGAGA AAGAGGAGGA CAAAGATCTG 
GAGGAGGTCA TGTTATCCAA GGGGTTTAAC CTCAACTGGT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814827.1
CDS	399..6983
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959373.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959373.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG ACAACCCCGG TGCTCGGCAG TAGCCGTTGG 
GAGCAGTGTC CCACACTGGA GAGGTCCGAG ACCCAATCCC GCTCTCCGGC TGGCCAATGG 
CAGCAGACCC TTGCTCAGCG TTCCTCCGAC CGCCCCTCGT CCCCCTCGTC CGATGCCCTT 
CGACAGAAGT ATATAAAGAT GTACACAGGT GGAGTGAGCT CATTGGCTGA GCAAATAGCC 
AATCAGCTTC AAAAGAAGGA AACGCCAAAA CCTCTGTTCG GGAAGAAAGT GGGCTCATTT 
AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG AGCGACGTCT GCTATTTCTG CCGCCGGAGG 
GTTTACGTCA TGGAGCGGCT CAGCGCCGAG GGCAAGTTCT TCCACCGGAG CTGCTTTAAG 
TGTGACTACT GTAGCACAAC GCTGCGCCTC TCATGTTATG CCTATGACCT TGAGGATGGA 
AAATTTTACT GCAAACCTCA CTACTGTTAC CGCCGGTCCG GATACAGCCA GAGGAAGAGG 
CCGGCCATGC CTGCACTTTC GGGAAAAGAA GTCACAAGGA CTGTGGAAGA CACCCCCGAC 
GCGGAAGACC AAGGACGAGC CCCGCTGCTG CCCGCCCCCG CTGCTGAGAA GACCCCAGGC 
CCCAGTGTGA ACGGCGTGGA AGATCACAGC GCCGCCAAGA GGCTGAGAGC CACCCCAGAG 
CGCATTGAGC TTGAGAATTA CCGCGTCTCC CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC 
GAGGAAACCC TGGCGGAGCA TAATCTGAGC AGCCTATTGG ATAAAGGGGA GGTGGAAGAG 
GTGGCCAGCA GCAGTTCCGA GTCCGAGATG GAGGAGGAGG AGGACGAAGA AGAAGAGGAG 
GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA GATCTGGGTG GGGTGCCGTG GAAAGAGGCC 
GTCAAGATCC ACGCCAAGTT AAAGGGTCAG GACATAGATG TGGAGGTTGT GGGAACCCAG 
GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG GAGGAGGAAG AAGAGGACGA AGAGGAGGAA 
GATGAAGAGG TATCAAGTGA AGAGGGTGAG TACTGCCCAT GGGAGAGAGA GCTTCAGCAG 
GGCCTGTGGA TACAGCGTAT CTCTGATGAA GATACAGTTG GTATATTTAA AGCCGGGAAC 
TTCAGACTCC AGCAAGCCCT CTCCCCTGTT GACCCCACGG AAATAAAGGC AGACGTGCGC 
TGGACACACG TCCGCGAGGT TAAGGAAGAG CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA 
GTGAGCATGT CCTCTCCTTC CAAAGCGCGT CATCTCCATT CCCACCGTCA CGACCCAGTG 
AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG 
GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA GCAGCTGGTG ATGGGGACAC CTCCGCAGAC 
CTTGGTGATG ATGATGACAT CCCGTCCGAC GCCGAGGCCG AAGAACGGCT CCAGCAGTCT 
CAGAGGTTTA AATTGAAGCT GGATGTAGCC CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG 
CCAGCAATTG CAGATTCTGA GAGCGTCCCC AGCCCAGTCT CTCTGGCACC GAAAGCCCCC 
GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG GAAGCAGAAA AGGTTCCTGT GATGTCTCCG 
AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC TTTGCGGATC CGTATCTGCC ATTGGACAGC 
AGCAACAAAG CCTTAGTGGA AGCAAAAACT CCAACTCCTC TATCTCCTGT TTGCTCCCAA 
CCAGTCCCAT CGCAAGAACC TGTTATCCCT ACATCTCCTG TGAAGTCTCC CTCAGTCCCA 
GCAAACACGC GCCCACTTAT ATCCCCATCA GCTCCTATTT GTTCCCAGCC ACTGCCGTCC 
TCAGAGGGTA CCCTAAATGC TACAGACAGG CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA 
ATCCCCTCTA CTTCTTTAAC CAAACTGCCT TTTACTGGTC ACGTCATGGG ATCTGAACAA 
TTAGTTAGCC CTAATGCAAA AGAGGTCCTG AAACGTATTG ACCTTATCGA AGAGTTCTGG 
ACAAAAAGCG CCGAGATCAG GCGCAGCTTA GGGCTGGCCC CCGTGGACAG AGATCGGGTA 
CATGAGATCA GTTTCCCTGC CCTAGCTTCA ACTCCTCTTC AGCCTCAGGC ACCTAAATCC 
AAACCTGAGG AGCAAAAGTC ACCGTCTCTG AAGCCACAAC AAGTTGCAAG AAGAGTGAAC 
CCTTCCCGGC TGGACATTGA ACTGCTCTCC CCTCTGACTC CAATATCCCC TTCTGAGAAG 
GAGCTGAAGA GCTCAGATGG TGAGAAGAAG GATATCTCCA CCAGCTCTGG CTTAGGCCTG 
AATGGCAGTT CCATAACACG TACTGCCGCC AGTGAAAGCT TTAACACCTC TGATTCAGCC 
ATGCTTACTC CACCATCCAG CCCTCCACCA CCTCCTCCCC AAGGGGAAGA GCCAGCTACT 
CTACGTAGAA GCCGTACTCC TTGCCAGAAT GGTCCAGAAT CTATCCCAGC CCCTGCCCTA 
GCACAGAGCA AGCCCCCTGA GCCCAAAAAG TCCCCCCGCT GGGAAGAATC TGTACGTAAG 
TCATTTGTAG AAAGTGTAGA CGAGATCCCC TTTGCCGATG ATGTGGAGGA CACGTACGAC 
GAAAGAACGG AGAATTCCAG CCTTCAAGAG AGGTTCTTTA CCCCACCAAC GAGTCGGGTT 
AAACCACATA GAATTGGTTT CTCCAAGGAA AATGGAGAGA TCCCATCTTC TTCGGAGAGA 
GTGCACCACA GAAAAAGGCT CTTGCCCGAC GTCACTCAGG AGGCCAAAGA ACGGGCAGAG 
GAGCGGATGA AAGCCCGGGA GAAGTCTGTG AAGAGCCAGG CACTGAAAGA CGCCATAGCA 
CAACAGCGGC ACAAAATGAA ACAACTAGAA CCAGGGGCAA GATCGGCCAA AGCTCTGCCA 
TCTACCGTTA TGTTCAGCGA TTACCCTGCA GTATCTCCTC TCCTGAGAAC CCAGACCGAG 
ACTCCCACCC TGAAGCTGGA TACCGACCAG GAGGTCTTTA TAACCCCCCC AGCAGCTCCT 
GACGGCTCCG TCACTTCCTC GGAAGGTTCA GGCGGGAAAC CCAAGAAAAG GCCGTCTATA 
TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC AAGGGGGAAA GCAGTCGGGC GTCCGATAAA 
CAGGCGACGG ACGAGAGCAC CAAACCCAAG TCCTTGTGGA AATCCGTCTT CTCCGGGTAT 
AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG GCGGAGGACA AGTCTACTCC GAGCACTCCA 
TCCAGCAGCA CCACCGTGGA CTCTGGGAAA GCCAAGGTGG CAAATATGAT CCGCGGAGCA 
GACCTGTCCC TCCGACGTCA CCTGAGCTTC TCGGAGGACT CGGACCTGTC CAGCGACGAT 
GTCTTGGAAA AATCCTCCCA GAAATCTAAG CGAGAGTCGG TGTATGTCCC CCACGCCTTG 
GCATTCAGAC GGTCATACTC ATCAAAGAAA ACCTACACGG ACGAGGAGCT AAGCGCTAAG 
CTAACAAGGC GGGTGCAGAA AGCGGCACGG AGACAGGCCA AGCAGGAGGA GCTAAAGCGG 
CTGCATCGGG CACAGATCAT TCAGCGCCAG CTGGAACAAG TGGAGGAAAA GCAGCGGCAG 
CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA GCTCTCCGGG GAGAAGCAGA CTATTGGGGA 
GAGTCTTATT ACAGTGACCT CATCGATTTG CACCTGGGTG TTGATCCCGT TGCCGGGACT 
CCGCGCCGTA GACCTCTCTC CTTTTGCCCC TGCTGTGCCC ACGAAGGGAT GGGAAAGAAG 
GACGACCCGA AACTCATGCA GGAATGGTTC CGCCTGGTGC AGGAGAAGAA CGCGCTGGTG 
CGATACGAAT CGGAGCTGAT GATATTTGCC CGCGAGTTGG AGTTGGAGGA CAGACAGAGC 
CGACTTCAGC AGGAGCTGCG GGAGCGAATG GCAGTGGAAG ATCAATTGAA GACGGAGCAG 
GAGCTGACGG ACGAGAAGAA GATCCTGAAC GAGATGCTGG ACGTGGTGGA ACAGAGAGAT 
GCTCTGGTGG CCCTGTTGGA GGAGCAGAGA CTGCGGGAGA AAGAGGAGGA CAAAGATCTG 
GAGGAGGTCA TGTTATCCAA GGGGTTTAAC CTCAACTGGT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959374.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814828.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X5, mRNA.

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CloneID	OXa33560
Clone ID Related Accession (Same CDS sequence)	XM_012959374.2
Accession Version	XM_012959374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814828.1
CDS	399..6956
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959374.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959374.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGTCGG TGTATGTCCC CCACGCCTTG GCATTCAGAC GGTCATACTC ATCAAAGAAA 
ACCTACACGG ACGAGGAGCT AAGCGCTAAG CTAACAAGGC GGGTGCAGAA AGCGGCACGG 
AGACAGGCCA AGCAGGAGGA GCTAAAGCGG CTGCATCGGG CACAGATCAT TCAGCGCCAG 
CTGGAACAAG TGGAGGAAAA GCAGCGGCAG CTGGAGGAGC GAGGGGTGGC TGTGGAGAAA 
GCTCTCCGGG GAGAAGCAGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959375.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814829.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X6, mRNA.

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CloneID	OXa33561
Clone ID Related Accession (Same CDS sequence)	XM_012959375.2
Accession Version	XM_012959375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGAAAA CCTACACGGA CGAGGAGCTA AGCGCTAAGC TAACAAGGCG GGTGCAGAAA 
GCGGCACGGA GACAGGCCAA GCAGGAGGAG CTAAAGCGGC TGCATCGGGC ACAGATCATT 
CAGCGCCAGC TGGAACAAGT GGAGGAAAAG CAGCGGCAGC TGGAGGAGCG AGGGGTGGCT 
GTGGAGAAAG CTCTCCGGGG AGAAGCAGAC TATTGGGGAG AGTCTTATTA CAGTGACCTC 
ATCGATTTGC ACCTGGGTGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814829.1
CDS	400..6957
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQRRDNQRPRSCPKKVITLNAGSSPVPLPVLQTTPVLGSSRWEQCPTLERSETQSRSPAGQWQQTLAQRSSDRPSSPSSDALRQKYIKMYTGGVSSLAEQIANQLQKKETPKPLFGKKVGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKREKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959375.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959375.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG CGCAGGGATA ACCAACGACC ACGGAGTTGC 
CCTAAAAAGG TCATCACCTT AAATGCGGGG TCTTCGCCTG TGCCTCTCCC TGTGCTGCAG 
ACAACCCCGG TGCTCGGCAG TAGCCGTTGG GAGCAGTGTC CCACACTGGA GAGGTCCGAG 
ACCCAATCCC GCTCTCCGGC TGGCCAATGG CAGCAGACCC TTGCTCAGCG TTCCTCCGAC 
CGCCCCTCGT CCCCCTCGTC CGATGCCCTT CGACAGAAGT ATATAAAGAT GTACACAGGT 
GGAGTGAGCT CATTGGCTGA GCAAATAGCC AATCAGCTTC AAAAGAAGGA AACGCCAAAA 
CCTCTGTTCG GGAAGAAAGT GGGCTCATTT AGGAAAGAGT TCCCCCAGAA CCTGGGAGGG 
AGCGACGTCT GCTATTTCTG CCGCCGGAGG GTTTACGTCA TGGAGCGGCT CAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTTAAG TGTGACTACT GTAGCACAAC GCTGCGCCTC 
TCATGTTATG CCTATGACCT TGAGGATGGA AAATTTTACT GCAAACCTCA CTACTGTTAC 
CGCCGGTCCG GATACAGCCA GAGGAAGAGG CCGGCCATGC CTGCACTTTC GGGAAAAGAA 
GTCACAAGGA CTGTGGAAGA CACCCCCGAC GCGGAAGACC AAGGACGAGC CCCGCTGCTG 
CCCGCCCCCG CTGCTGAGAA GACCCCAGGC CCCAGTGTGA ACGGCGTGGA AGATCACAGC 
GCCGCCAAGA GGCTGAGAGC CACCCCAGAG CGCATTGAGC TTGAGAATTA CCGCGTCTCC 
CTGCAGAAGG AGGAGCTGGA GGAGGTGCCC GAGGAAACCC TGGCGGAGCA TAATCTGAGC 
AGCCTATTGG ATAAAGGGGA GGTGGAAGAG GTGGCCAGCA GCAGTTCCGA GTCCGAGATG 
GAGGAGGAGG AGGACGAAGA AGAAGAGGAG GAGGAGCCAG GAGAGTGCCT TCCCCCCTCA 
GATCTGGGTG GGGTGCCGTG GAAAGAGGCC GTCAAGATCC ACGCCAAGTT AAAGGGTCAG 
GACATAGATG TGGAGGTTGT GGGAACCCAG GGGGCTGGTG GCAGTGAAGA GGAGGAGGAG 
GAGGAGGAAG AAGAGGACGA AGAGGAGGAA GATGAAGAGG TATCAAGTGA AGAGGGTGAG 
TACTGCCCAT GGGAGAGAGA GCTTCAGCAG GGCCTGTGGA TACAGCGTAT CTCTGATGAA 
GATACAGTTG GTATATTTAA AGCCGGGAAC TTCAGACTCC AGCAAGCCCT CTCCCCTGTT 
GACCCCACGG AAATAAAGGC AGACGTGCGC TGGACACACG TCCGCGAGGT TAAGGAAGAG 
CGGAGAGGGT CTAAGGAAGA GAGGGGAGAA GTGAGCATGT CCTCTCCTTC CAAAGCGCGT 
CATCTCCATT CCCACCGTCA CGACCCAGTG AGAGCGTGGC TGGAGTCCGT ATCAGGAGCT 
ACTGGCGAGT ATGAAGAAGG TGAGGAGGAG GAGCTGGGTA CTTGCCTGGC GCAGGCTGAA 
GCAGCTGGTG ATGGGGACAC CTCCGCAGAC CTTGGTGATG ATGATGACAT CCCGTCCGAC 
GCCGAGGCCG AAGAACGGCT CCAGCAGTCT CAGAGGTTTA AATTGAAGCT GGATGTAGCC 
CAAGAGCCTA AGGTGGAGCA ACCCGTCCTG CCAGCAATTG CAGATTCTGA GAGCGTCCCC 
AGCCCAGTCT CTCTGGCACC GAAAGCCCCC GTGCTGTCTC CAAGAAGGAC ACTCAAAGCG 
GAAGCAGAAA AGGTTCCTGT GATGTCTCCG AAGGCAGTGA CCTCTCCTGG GGGTCGGTTC 
TTTGCGGATC CGTATCTGCC ATTGGACAGC AGCAACAAAG CCTTAGTGGA AGCAAAAACT 
CCAACTCCTC TATCTCCTGT TTGCTCCCAA CCAGTCCCAT CGCAAGAACC TGTTATCCCT 
ACATCTCCTG TGAAGTCTCC CTCAGTCCCA GCAAACACGC GCCCACTTAT ATCCCCATCA 
GCTCCTATTT GTTCCCAGCC ACTGCCGTCC TCAGAGGGTA CCCTAAATGC TACAGACAGG 
CCCCCCGTGC GTCAGCAGCC ATCCCCTCTA ATCCCCTCTA CTTCTTTAAC CAAACTGCCT 
TTTACTGGTC ACGTCATGGG ATCTGAACAA TTAGTTAGCC CTAATGCAAA AGAGGTCCTG 
AAACGTATTG ACCTTATCGA AGAGTTCTGG ACAAAAAGCG CCGAGATCAG GCGCAGCTTA 
GGGCTGGCCC CCGTGGACAG AGATCGGGTA CATGAGATCA GTTTCCCTGC CCTAGCTTCA 
ACTCCTCTTC AGCCTCAGGC ACCTAAATCC AAACCTGAGG AGCAAAAGTC ACCGTCTCTG 
AAGCCACAAC AAGTTGCAAG AAGAGTGAAC CCTTCCCGGC TGGACATTGA ACTGCTCTCC 
CCTCTGACTC CAATATCCCC TTCTGAGAAG GAGCTGAAGA GCTCAGATGG TGAGAAGAAG 
GATATCTCCA CCAGCTCTGG CTTAGGCCTG AATGGCAGTT CCATAACACG TACTGCCGCC 
AGTGAAAGCT TTAACACCTC TGATTCAGCC ATGCTTACTC CACCATCCAG CCCTCCACCA 
CCTCCTCCCC AAGGGGAAGA GCCAGCTACT CTACGTAGAA GCCGTACTCC TTGCCAGAAT 
GGTCCAGAAT CTATCCCAGC CCCTGCCCTA GCACAGAGCA AGCCCCCTGA GCCCAAAAAG 
TCCCCCCGCT GGGAAGAATC TGTACGTAAG TCATTTGTAG AAAGTGTAGA CGAGATCCCC 
TTTGCCGATG ATGTGGAGGA CACGTACGAC GAAAGAACGG AGAATTCCAG CCTTCAAGAG 
AGGTTCTTTA CCCCACCAAC GAGTCGGGTT AAACCACATA GAATTGGTTT CTCCAAGGAA 
AATGGAGAGA TCCCATCTTC TTCGGAGAGA GTGCACCACA GAAAAAGGCT CTTGCCCGAC 
GTCACTCAGG AGGCCAAAGA ACGGGCAGAG GAGCGGATGA AAGCCCGGGA GAAGTCTGTG 
AAGAGCCAGG CACTGAAAGA CGCCATAGCA CAACAGCGGC ACAAAATGAA ACAACTAGAA 
CCAGGGGCAA GATCGGCCAA AGCTCTGCCA TCTACCGTTA TGTTCAGCGA TTACCCTGCA 
GTATCTCCTC TCCTGAGAAC CCAGACCGAG ACTCCCACCC TGAAGCTGGA TACCGACCAG 
GAGGTCTTTA TAACCCCCCC AGCAGCTCCT GACGGCTCCG TCACTTCCTC GGAAGGTTCA 
GGCGGGAAAC CCAAGAAAAG GCCGTCTATA TTCTCCCCGA GGAAGAAAGA GAAAAAGTCC 
AAGGGGGAAA GCAGTCGGGC GTCCGATAAA CAGGCGACGG ACGAGAGCAC CAAACCCAAG 
TCCTTGTGGA AATCCGTCTT CTCCGGGTAT AAGAAGGAAA AGAAGAAGAA GCAGCAGCAG 
GCGGAGGACA AGTCTACTCC GAGCACTCCA TCCAGCAGCA CCACCGTGGA CTCTGGGAAA 
GCCAAGGTGG CAAATATGAT CCGCGGAGCA GACCTGTCCC TCCGACGTCA CCTGAGCTTC 
TCGGAGGACT CGGACCTGTC CAGCGACGAT GTCTTGGAAA AATCCTCCCA GAAATCTAAG 
CGAGAGAAAA CCTACACGGA CGAGGAGCTA AGCGCTAAGC TAACAAGGCG GGTGCAGAAA 
GCGGCACGGA GACAGGCCAA GCAGGAGGAG CTAAAGCGGC TGCATCGGGC ACAGATCATT 
CAGCGCCAGC TGGAACAAGT GGAGGAAAAG CAGCGGCAGC TGGAGGAGCG AGGGGTGGCT 
GTGGAGAAAG CTCTCCGGGG AGAAGCAGAC TATTGGGGAG AGTCTTATTA CAGTGACCTC 
ATCGATTTGC ACCTGGGTGG GATGGGAAAG AAGGACGACC CGAAACTCAT GCAGGAATGG 
TTCCGCCTGG TGCAGGAGAA GAACGCGCTG GTGCGATACG AATCGGAGCT GATGATATTT 
GCCCGCGAGT TGGAGTTGGA GGACAGACAG AGCCGACTTC AGCAGGAGCT GCGGGAGCGA 
ATGGCAGTGG AAGATCAATT GAAGACGGAG CAGGAGCTGA CGGACGAGAA GAAGATCCTG 
AACGAGATGC TGGACGTGGT GGAACAGAGA GATGCTCTGG TGGCCCTGTT GGAGGAGCAG 
AGACTGCGGG AGAAAGAGGA GGACAAAGAT CTGGAGGAGG TCATGTTATC CAAGGGGTTT 
AACCTCAACT GGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959380.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814834.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X8, mRNA.

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CloneID	OXa33566
Clone ID Related Accession (Same CDS sequence)	XM_012959380.2
Accession Version	XM_012959380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGAGGG TGAGTACTGC 
CCATGGGAGA GAGAGCTTCA GCAGGGCCTG TGGATACAGC GTATCTCTGA TGAAGATACA 
GTTGGTATAT TTAAAGCCGG GAACTTCAGA CTCCAGCAAG CCCTCTCCCC TGTTGACCCC 
ACGGAAATAA AGGCAGACGT GCGCTGGACA CACGTCCGCG AGGTTAAGGA AGAGCGGAGA 
GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC ATGTCCTCTC CTTCCAAAGC GCGTCATCTC 
CATTCCCACC GTCACGACCC AGTGAGAGCG TGGCTGGAGT CCGTATCAGG AGCTACTGGC 
GAGTATGAAG AAGGTGAGGA GGAGGAGCTG GGTACTTGCC TGGCGCAGGC TGAAGCAGCT 
GGTGATGGGG ACACCTCCGC AGACCTTGGT GATGATGATG ACATCCCGTC CGACGCCGAG 
GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG TTTAAATTGA AGCTGGATGT AGCCCAAGAG 
CCTAAGGTGG AGCAACCCGT CCTGCCAGCA ATTGCAGATT CTGAGAGCGT CCCCAGCCCA 
GTCTCTCTGG CACCGAAAGC CCCCGTGCTG TCTCCAAGAA GGACACTCAA AGCGGAAGCA 
GAAAAGGTTC CTGTGATGTC TCCGAAGGCA GTGACCTCTC CTGGGGGTCG GTTCTTTGCG 
GATCCGTATC TGCCATTGGA CAGCAGCAAC AAAGCCTTAG TGGAAGCAAA AACTCCAACT 
CCTCTATCTC CTGTTTGCTC CCAACCAGTC CCATCGCAAG AACCTGTTAT CCCTACATCT 
CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC ACGCGCCCAC TTATATCCCC ATCAGCTCCT 
ATTTGTTCCC AGCCACTGCC GTCCTCAGAG GGTACCCTAA ATGCTACAGA CAGGCCCCCC 
GTGCGTCAGC AGCCATCCCC TCTAATCCCC TCTACTTCTT TAACCAAACT GCCTTTTACT 
GGTCACGTCA TGGGATCTGA ACAATTAGTT AGCCCTAATG CAAAAGAGGT CCTGAAACGT 
ATTGACCTTA TCGAAGAGTT CTGGACAAAA AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG 
GCCCCCGTGG ACAGAGATCG GGTACATGAG ATCAGTTTCC CTGCCCTAGC TTCAACTCCT 
CTTCAGCCTC AGGCACCTAA ATCCAAACCT GAGGAGCAAA AGTCACCGTC TCTGAAGCCA 
CAACAAGTTG CAAGAAGAGT GAACCCTTCC CGGCTGGACA TTGAACTGCT CTCCCCTCTG 
ACTCCAATAT CCCCTTCTGA GAAGGAGCTG AAGAGCTCAG ATGGTGAGAA GAAGGATATC 
TCCACCAGCT CTGGCTTAGG CCTGAATGGC AGTTCCATAA CACGTACTGC CGCCAGTGAA 
AGCTTTAACA CCTCTGATTC AGCCATGCTT ACTCCACCAT CCAGCCCTCC ACCACCTCCT 
CCCCAAGGGG AAGAGCCAGC TACTCTACGT AGAAGCCGTA CTCCTTGCCA GAATGGTCCA 
GAATCTATCC CAGCCCCTGC CCTAGCACAG AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC 
CGCTGGGAAG AATCTGTACG TAAGTCATTT GTAGAAAGTG TAGACGAGAT CCCCTTTGCC 
GATGATGTGG AGGACACGTA CGACGAAAGA ACGGAGAATT CCAGCCTTCA AGAGAGGTTC 
TTTACCCCAC CAACGAGTCG GGTTAAACCA CATAGAATTG GTTTCTCCAA GGAAAATGGA 
GAGATCCCAT CTTCTTCGGA GAGAGTGCAC CACAGAAAAA GGCTCTTGCC CGACGTCACT 
CAGGAGGCCA AAGAACGGGC AGAGGAGCGG ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC 
CAGGCACTGA AAGACGCCAT AGCACAACAG CGGCACAAAA TGAAACAACT AGAACCAGGG 
GCAAGATCGG CCAAAGCTCT GCCATCTACC GTTATGTTCA GCGATTACCC TGCAGTATCT 
CCTCTCCTGA GAACCCAGAC CGAGACTCCC ACCCTGAAGC TGGATACCGA CCAGGAGGTC 
TTTATAACCC CCCCAGCAGC TCCTGACGGC TCCGTCACTT CCTCGGAAGG TTCAGGCGGG 
AAACCCAAGA AAAGGCCGTC TATATTCTCC CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG 
GAAAGCAGTC GGGCGTCCGA TAAACAGGCG ACGGACGAGA GCACCAAACC CAAGTCCTTG 
TGGAAATCCG TCTTCTCCGG GTATAAGAAG GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG 
GACAAGTCTA CTCCGAGCAC TCCATCCAGC AGCACCACCG TGGACTCTGG GAAAGCCAAG 
GTGGCAAATA TGATCCGCGG AGCAGACCTG TCCCTCCGAC GTCACCTGAG CTTCTCGGAG 
GACTCGGACC TGTCCAGCGA CGATGTCTTG GAAAAATCCT CCCAGAAATC TAAGCGAGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGTTGATC CCGTTGCCGG GACTCCGCGC CGTAGACCTC TCTCCTTTTG CCCCTGCTGT 
GCCCACGAAG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814834.1
CDS	397..6645
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959380.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959380.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGAGGG TGAGTACTGC 
CCATGGGAGA GAGAGCTTCA GCAGGGCCTG TGGATACAGC GTATCTCTGA TGAAGATACA 
GTTGGTATAT TTAAAGCCGG GAACTTCAGA CTCCAGCAAG CCCTCTCCCC TGTTGACCCC 
ACGGAAATAA AGGCAGACGT GCGCTGGACA CACGTCCGCG AGGTTAAGGA AGAGCGGAGA 
GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC ATGTCCTCTC CTTCCAAAGC GCGTCATCTC 
CATTCCCACC GTCACGACCC AGTGAGAGCG TGGCTGGAGT CCGTATCAGG AGCTACTGGC 
GAGTATGAAG AAGGTGAGGA GGAGGAGCTG GGTACTTGCC TGGCGCAGGC TGAAGCAGCT 
GGTGATGGGG ACACCTCCGC AGACCTTGGT GATGATGATG ACATCCCGTC CGACGCCGAG 
GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG TTTAAATTGA AGCTGGATGT AGCCCAAGAG 
CCTAAGGTGG AGCAACCCGT CCTGCCAGCA ATTGCAGATT CTGAGAGCGT CCCCAGCCCA 
GTCTCTCTGG CACCGAAAGC CCCCGTGCTG TCTCCAAGAA GGACACTCAA AGCGGAAGCA 
GAAAAGGTTC CTGTGATGTC TCCGAAGGCA GTGACCTCTC CTGGGGGTCG GTTCTTTGCG 
GATCCGTATC TGCCATTGGA CAGCAGCAAC AAAGCCTTAG TGGAAGCAAA AACTCCAACT 
CCTCTATCTC CTGTTTGCTC CCAACCAGTC CCATCGCAAG AACCTGTTAT CCCTACATCT 
CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC ACGCGCCCAC TTATATCCCC ATCAGCTCCT 
ATTTGTTCCC AGCCACTGCC GTCCTCAGAG GGTACCCTAA ATGCTACAGA CAGGCCCCCC 
GTGCGTCAGC AGCCATCCCC TCTAATCCCC TCTACTTCTT TAACCAAACT GCCTTTTACT 
GGTCACGTCA TGGGATCTGA ACAATTAGTT AGCCCTAATG CAAAAGAGGT CCTGAAACGT 
ATTGACCTTA TCGAAGAGTT CTGGACAAAA AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG 
GCCCCCGTGG ACAGAGATCG GGTACATGAG ATCAGTTTCC CTGCCCTAGC TTCAACTCCT 
CTTCAGCCTC AGGCACCTAA ATCCAAACCT GAGGAGCAAA AGTCACCGTC TCTGAAGCCA 
CAACAAGTTG CAAGAAGAGT GAACCCTTCC CGGCTGGACA TTGAACTGCT CTCCCCTCTG 
ACTCCAATAT CCCCTTCTGA GAAGGAGCTG AAGAGCTCAG ATGGTGAGAA GAAGGATATC 
TCCACCAGCT CTGGCTTAGG CCTGAATGGC AGTTCCATAA CACGTACTGC CGCCAGTGAA 
AGCTTTAACA CCTCTGATTC AGCCATGCTT ACTCCACCAT CCAGCCCTCC ACCACCTCCT 
CCCCAAGGGG AAGAGCCAGC TACTCTACGT AGAAGCCGTA CTCCTTGCCA GAATGGTCCA 
GAATCTATCC CAGCCCCTGC CCTAGCACAG AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC 
CGCTGGGAAG AATCTGTACG TAAGTCATTT GTAGAAAGTG TAGACGAGAT CCCCTTTGCC 
GATGATGTGG AGGACACGTA CGACGAAAGA ACGGAGAATT CCAGCCTTCA AGAGAGGTTC 
TTTACCCCAC CAACGAGTCG GGTTAAACCA CATAGAATTG GTTTCTCCAA GGAAAATGGA 
GAGATCCCAT CTTCTTCGGA GAGAGTGCAC CACAGAAAAA GGCTCTTGCC CGACGTCACT 
CAGGAGGCCA AAGAACGGGC AGAGGAGCGG ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC 
CAGGCACTGA AAGACGCCAT AGCACAACAG CGGCACAAAA TGAAACAACT AGAACCAGGG 
GCAAGATCGG CCAAAGCTCT GCCATCTACC GTTATGTTCA GCGATTACCC TGCAGTATCT 
CCTCTCCTGA GAACCCAGAC CGAGACTCCC ACCCTGAAGC TGGATACCGA CCAGGAGGTC 
TTTATAACCC CCCCAGCAGC TCCTGACGGC TCCGTCACTT CCTCGGAAGG TTCAGGCGGG 
AAACCCAAGA AAAGGCCGTC TATATTCTCC CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG 
GAAAGCAGTC GGGCGTCCGA TAAACAGGCG ACGGACGAGA GCACCAAACC CAAGTCCTTG 
TGGAAATCCG TCTTCTCCGG GTATAAGAAG GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG 
GACAAGTCTA CTCCGAGCAC TCCATCCAGC AGCACCACCG TGGACTCTGG GAAAGCCAAG 
GTGGCAAATA TGATCCGCGG AGCAGACCTG TCCCTCCGAC GTCACCTGAG CTTCTCGGAG 
GACTCGGACC TGTCCAGCGA CGATGTCTTG GAAAAATCCT CCCAGAAATC TAAGCGAGAG 
TCGGTGTATG TCCCCCACGC CTTGGCATTC AGACGGTCAT ACTCATCAAA GAAAACCTAC 
ACGGACGAGG AGCTAAGCGC TAAGCTAACA AGGCGGGTGC AGAAAGCGGC ACGGAGACAG 
GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT CGGGCACAGA TCATTCAGCG CCAGCTGGAA 
CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC 
CGGGGAGAAG CAGACTATTG GGGAGAGTCT TATTACAGTG ACCTCATCGA TTTGCACCTG 
GGTGTTGATC CCGTTGCCGG GACTCCGCGC CGTAGACCTC TCTCCTTTTG CCCCTGCTGT 
GCCCACGAAG GGATGGGAAA GAAGGACGAC CCGAAACTCA TGCAGGAATG GTTCCGCCTG 
GTGCAGGAGA AGAACGCGCT GGTGCGATAC GAATCGGAGC TGATGATATT TGCCCGCGAG 
TTGGAGTTGG AGGACAGACA GAGCCGACTT CAGCAGGAGC TGCGGGAGCG AATGGCAGTG 
GAAGATCAAT TGAAGACGGA GCAGGAGCTG ACGGACGAGA AGAAGATCCT GAACGAGATG 
CTGGACGTGG TGGAACAGAG AGATGCTCTG GTGGCCCTGT TGGAGGAGCA GAGACTGCGG 
GAGAAAGAGG AGGACAAAGA TCTGGAGGAG GTCATGTTAT CCAAGGGGTT TAACCTCAAC 
TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959381.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814835.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X9, mRNA.

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CloneID	OXa33567
Clone ID Related Accession (Same CDS sequence)	XM_012959381.2
Accession Version	XM_012959381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGCCGG GAACTTCAGA 
CTCCAGCAAG CCCTCTCCCC TGTTGACCCC ACGGAAATAA AGGCAGACGT GCGCTGGACA 
CACGTCCGCG AGGTTAAGGA AGAGCGGAGA GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC 
ATGTCCTCTC CTTCCAAAGC GCGTCATCTC CATTCCCACC GTCACGACCC AGTGAGAGCG 
TGGCTGGAGT CCGTATCAGG AGCTACTGGC GAGTATGAAG AAGGTGAGGA GGAGGAGCTG 
GGTACTTGCC TGGCGCAGGC TGAAGCAGCT GGTGATGGGG ACACCTCCGC AGACCTTGGT 
GATGATGATG ACATCCCGTC CGACGCCGAG GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG 
TTTAAATTGA AGCTGGATGT AGCCCAAGAG CCTAAGGTGG AGCAACCCGT CCTGCCAGCA 
ATTGCAGATT CTGAGAGCGT CCCCAGCCCA GTCTCTCTGG CACCGAAAGC CCCCGTGCTG 
TCTCCAAGAA GGACACTCAA AGCGGAAGCA GAAAAGGTTC CTGTGATGTC TCCGAAGGCA 
GTGACCTCTC CTGGGGGTCG GTTCTTTGCG GATCCGTATC TGCCATTGGA CAGCAGCAAC 
AAAGCCTTAG TGGAAGCAAA AACTCCAACT CCTCTATCTC CTGTTTGCTC CCAACCAGTC 
CCATCGCAAG AACCTGTTAT CCCTACATCT CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC 
ACGCGCCCAC TTATATCCCC ATCAGCTCCT ATTTGTTCCC AGCCACTGCC GTCCTCAGAG 
GGTACCCTAA ATGCTACAGA CAGGCCCCCC GTGCGTCAGC AGCCATCCCC TCTAATCCCC 
TCTACTTCTT TAACCAAACT GCCTTTTACT GGTCACGTCA TGGGATCTGA ACAATTAGTT 
AGCCCTAATG CAAAAGAGGT CCTGAAACGT ATTGACCTTA TCGAAGAGTT CTGGACAAAA 
AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG GCCCCCGTGG ACAGAGATCG GGTACATGAG 
ATCAGTTTCC CTGCCCTAGC TTCAACTCCT CTTCAGCCTC AGGCACCTAA ATCCAAACCT 
GAGGAGCAAA AGTCACCGTC TCTGAAGCCA CAACAAGTTG CAAGAAGAGT GAACCCTTCC 
CGGCTGGACA TTGAACTGCT CTCCCCTCTG ACTCCAATAT CCCCTTCTGA GAAGGAGCTG 
AAGAGCTCAG ATGGTGAGAA GAAGGATATC TCCACCAGCT CTGGCTTAGG CCTGAATGGC 
AGTTCCATAA CACGTACTGC CGCCAGTGAA AGCTTTAACA CCTCTGATTC AGCCATGCTT 
ACTCCACCAT CCAGCCCTCC ACCACCTCCT CCCCAAGGGG AAGAGCCAGC TACTCTACGT 
AGAAGCCGTA CTCCTTGCCA GAATGGTCCA GAATCTATCC CAGCCCCTGC CCTAGCACAG 
AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC CGCTGGGAAG AATCTGTACG TAAGTCATTT 
GTAGAAAGTG TAGACGAGAT CCCCTTTGCC GATGATGTGG AGGACACGTA CGACGAAAGA 
ACGGAGAATT CCAGCCTTCA AGAGAGGTTC TTTACCCCAC CAACGAGTCG GGTTAAACCA 
CATAGAATTG GTTTCTCCAA GGAAAATGGA GAGATCCCAT CTTCTTCGGA GAGAGTGCAC 
CACAGAAAAA GGCTCTTGCC CGACGTCACT CAGGAGGCCA AAGAACGGGC AGAGGAGCGG 
ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC CAGGCACTGA AAGACGCCAT AGCACAACAG 
CGGCACAAAA TGAAACAACT AGAACCAGGG GCAAGATCGG CCAAAGCTCT GCCATCTACC 
GTTATGTTCA GCGATTACCC TGCAGTATCT CCTCTCCTGA GAACCCAGAC CGAGACTCCC 
ACCCTGAAGC TGGATACCGA CCAGGAGGTC TTTATAACCC CCCCAGCAGC TCCTGACGGC 
TCCGTCACTT CCTCGGAAGG TTCAGGCGGG AAACCCAAGA AAAGGCCGTC TATATTCTCC 
CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG GAAAGCAGTC GGGCGTCCGA TAAACAGGCG 
ACGGACGAGA GCACCAAACC CAAGTCCTTG TGGAAATCCG TCTTCTCCGG GTATAAGAAG 
GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG GACAAGTCTA CTCCGAGCAC TCCATCCAGC 
AGCACCACCG TGGACTCTGG GAAAGCCAAG GTGGCAAATA TGATCCGCGG AGCAGACCTG 
TCCCTCCGAC GTCACCTGAG CTTCTCGGAG GACTCGGACC TGTCCAGCGA CGATGTCTTG 
GAAAAATCCT CCCAGAAATC TAAGCGAGAG TCGGTGTATG TCCCCCACGC CTTGGCATTC 
AGACGGTCAT ACTCATCAAA GAAAACCTAC ACGGACGAGG AGCTAAGCGC TAAGCTAACA 
AGGCGGGTGC AGAAAGCGGC ACGGAGACAG GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT 
CGGGCACAGA TCATTCAGCG CCAGCTGGAA CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG 
GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC CGGGGAGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA TTTGCACCTG GGTGTTGATC CCGTTGCCGG GACTCCGCGC 
CGTAGACCTC TCTCCTTTTG CCCCTGCTGT GCCCACGAAG GGATGGGAAA GAAGGACGAC 
CCGAAACTCA TGCAGGAATG GTTCCGCCTG GTGCAGGAGA AGAACGCGCT GGTGCGATAC 
GAATCGGAGC TGATGATATT TGCCCGCGAG TTGGAGTTGG AGGACAGACA GAGCCGACTT 
CAGCAGGAGC TGCGGGAGCG AATGGCAGTG GAAGATCAAT TGAAGACGGA GCAGGAGCTG 
ACGGACGAGA AGAAGATCCT GAACGAGATG CTGGACGTGG TGGAACAGAG AGATGCTCTG 
GTGGCCCTGT TGGAGGAGCA GAGACTGCGG GAGAAAGAGG AGGACAAAGA TCTGGAGGAG 
GTCATGTTAT CCAAGGGGTT TAACCTCAAC TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814835.1
CDS	397..6555
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959381.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959381.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGCCGG GAACTTCAGA 
CTCCAGCAAG CCCTCTCCCC TGTTGACCCC ACGGAAATAA AGGCAGACGT GCGCTGGACA 
CACGTCCGCG AGGTTAAGGA AGAGCGGAGA GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC 
ATGTCCTCTC CTTCCAAAGC GCGTCATCTC CATTCCCACC GTCACGACCC AGTGAGAGCG 
TGGCTGGAGT CCGTATCAGG AGCTACTGGC GAGTATGAAG AAGGTGAGGA GGAGGAGCTG 
GGTACTTGCC TGGCGCAGGC TGAAGCAGCT GGTGATGGGG ACACCTCCGC AGACCTTGGT 
GATGATGATG ACATCCCGTC CGACGCCGAG GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG 
TTTAAATTGA AGCTGGATGT AGCCCAAGAG CCTAAGGTGG AGCAACCCGT CCTGCCAGCA 
ATTGCAGATT CTGAGAGCGT CCCCAGCCCA GTCTCTCTGG CACCGAAAGC CCCCGTGCTG 
TCTCCAAGAA GGACACTCAA AGCGGAAGCA GAAAAGGTTC CTGTGATGTC TCCGAAGGCA 
GTGACCTCTC CTGGGGGTCG GTTCTTTGCG GATCCGTATC TGCCATTGGA CAGCAGCAAC 
AAAGCCTTAG TGGAAGCAAA AACTCCAACT CCTCTATCTC CTGTTTGCTC CCAACCAGTC 
CCATCGCAAG AACCTGTTAT CCCTACATCT CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC 
ACGCGCCCAC TTATATCCCC ATCAGCTCCT ATTTGTTCCC AGCCACTGCC GTCCTCAGAG 
GGTACCCTAA ATGCTACAGA CAGGCCCCCC GTGCGTCAGC AGCCATCCCC TCTAATCCCC 
TCTACTTCTT TAACCAAACT GCCTTTTACT GGTCACGTCA TGGGATCTGA ACAATTAGTT 
AGCCCTAATG CAAAAGAGGT CCTGAAACGT ATTGACCTTA TCGAAGAGTT CTGGACAAAA 
AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG GCCCCCGTGG ACAGAGATCG GGTACATGAG 
ATCAGTTTCC CTGCCCTAGC TTCAACTCCT CTTCAGCCTC AGGCACCTAA ATCCAAACCT 
GAGGAGCAAA AGTCACCGTC TCTGAAGCCA CAACAAGTTG CAAGAAGAGT GAACCCTTCC 
CGGCTGGACA TTGAACTGCT CTCCCCTCTG ACTCCAATAT CCCCTTCTGA GAAGGAGCTG 
AAGAGCTCAG ATGGTGAGAA GAAGGATATC TCCACCAGCT CTGGCTTAGG CCTGAATGGC 
AGTTCCATAA CACGTACTGC CGCCAGTGAA AGCTTTAACA CCTCTGATTC AGCCATGCTT 
ACTCCACCAT CCAGCCCTCC ACCACCTCCT CCCCAAGGGG AAGAGCCAGC TACTCTACGT 
AGAAGCCGTA CTCCTTGCCA GAATGGTCCA GAATCTATCC CAGCCCCTGC CCTAGCACAG 
AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC CGCTGGGAAG AATCTGTACG TAAGTCATTT 
GTAGAAAGTG TAGACGAGAT CCCCTTTGCC GATGATGTGG AGGACACGTA CGACGAAAGA 
ACGGAGAATT CCAGCCTTCA AGAGAGGTTC TTTACCCCAC CAACGAGTCG GGTTAAACCA 
CATAGAATTG GTTTCTCCAA GGAAAATGGA GAGATCCCAT CTTCTTCGGA GAGAGTGCAC 
CACAGAAAAA GGCTCTTGCC CGACGTCACT CAGGAGGCCA AAGAACGGGC AGAGGAGCGG 
ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC CAGGCACTGA AAGACGCCAT AGCACAACAG 
CGGCACAAAA TGAAACAACT AGAACCAGGG GCAAGATCGG CCAAAGCTCT GCCATCTACC 
GTTATGTTCA GCGATTACCC TGCAGTATCT CCTCTCCTGA GAACCCAGAC CGAGACTCCC 
ACCCTGAAGC TGGATACCGA CCAGGAGGTC TTTATAACCC CCCCAGCAGC TCCTGACGGC 
TCCGTCACTT CCTCGGAAGG TTCAGGCGGG AAACCCAAGA AAAGGCCGTC TATATTCTCC 
CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG GAAAGCAGTC GGGCGTCCGA TAAACAGGCG 
ACGGACGAGA GCACCAAACC CAAGTCCTTG TGGAAATCCG TCTTCTCCGG GTATAAGAAG 
GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG GACAAGTCTA CTCCGAGCAC TCCATCCAGC 
AGCACCACCG TGGACTCTGG GAAAGCCAAG GTGGCAAATA TGATCCGCGG AGCAGACCTG 
TCCCTCCGAC GTCACCTGAG CTTCTCGGAG GACTCGGACC TGTCCAGCGA CGATGTCTTG 
GAAAAATCCT CCCAGAAATC TAAGCGAGAG TCGGTGTATG TCCCCCACGC CTTGGCATTC 
AGACGGTCAT ACTCATCAAA GAAAACCTAC ACGGACGAGG AGCTAAGCGC TAAGCTAACA 
AGGCGGGTGC AGAAAGCGGC ACGGAGACAG GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT 
CGGGCACAGA TCATTCAGCG CCAGCTGGAA CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG 
GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC CGGGGAGAAG CAGACTATTG GGGAGAGTCT 
TATTACAGTG ACCTCATCGA TTTGCACCTG GGTGTTGATC CCGTTGCCGG GACTCCGCGC 
CGTAGACCTC TCTCCTTTTG CCCCTGCTGT GCCCACGAAG GGATGGGAAA GAAGGACGAC 
CCGAAACTCA TGCAGGAATG GTTCCGCCTG GTGCAGGAGA AGAACGCGCT GGTGCGATAC 
GAATCGGAGC TGATGATATT TGCCCGCGAG TTGGAGTTGG AGGACAGACA GAGCCGACTT 
CAGCAGGAGC TGCGGGAGCG AATGGCAGTG GAAGATCAAT TGAAGACGGA GCAGGAGCTG 
ACGGACGAGA AGAAGATCCT GAACGAGATG CTGGACGTGG TGGAACAGAG AGATGCTCTG 
GTGGCCCTGT TGGAGGAGCA GAGACTGCGG GAGAAAGAGG AGGACAAAGA TCTGGAGGAG 
GTCATGTTAT CCAAGGGGTT TAACCTCAAC TGGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_012959383.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814837.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X11, mRNA.

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CloneID	OXa33569
Clone ID Related Accession (Same CDS sequence)	XM_012959383.2
Accession Version	XM_012959383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGCCGG GAACTTCAGA 
CTCCAGCAAG CCCTCTCCCC TGTTGACCCC ACGGAAATAA AGGCAGACGT GCGCTGGACA 
CACGTCCGCG AGGTTAAGGA AGAGCGGAGA GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC 
ATGTCCTCTC CTTCCAAAGC GCGTCATCTC CATTCCCACC GTCACGACCC AGTGAGAGCG 
TGGCTGGAGT CCGTATCAGG AGCTACTGGC GAGTATGAAG AAGGTGAGGA GGAGGAGCTG 
GGTACTTGCC TGGCGCAGGC TGAAGCAGCT GGTGATGGGG ACACCTCCGC AGACCTTGGT 
GATGATGATG ACATCCCGTC CGACGCCGAG GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG 
TTTAAATTGA AGCTGGATGT AGCCCAAGAG CCTAAGGTGG AGCAACCCGT CCTGCCAGCA 
ATTGCAGATT CTGAGAGCGT CCCCAGCCCA GTCTCTCTGG CACCGAAAGC CCCCGTGCTG 
TCTCCAAGAA GGACACTCAA AGCGGAAGCA GAAAAGGTTC CTGTGATGTC TCCGAAGGCA 
GTGACCTCTC CTGGGGGTCG GTTCTTTGCG GATCCGTATC TGCCATTGGA CAGCAGCAAC 
AAAGCCTTAG TGGAAGCAAA AACTCCAACT CCTCTATCTC CTGTTTGCTC CCAACCAGTC 
CCATCGCAAG AACCTGTTAT CCCTACATCT CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC 
ACGCGCCCAC TTATATCCCC ATCAGCTCCT ATTTGTTCCC AGCCACTGCC GTCCTCAGAG 
GGTACCCTAA ATGCTACAGA CAGGCCCCCC GTGCGTCAGC AGCCATCCCC TCTAATCCCC 
TCTACTTCTT TAACCAAACT GCCTTTTACT GGTCACGTCA TGGGATCTGA ACAATTAGTT 
AGCCCTAATG CAAAAGAGGT CCTGAAACGT ATTGACCTTA TCGAAGAGTT CTGGACAAAA 
AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG GCCCCCGTGG ACAGAGATCG GGTACATGAG 
ATCAGTTTCC CTGCCCTAGC TTCAACTCCT CTTCAGCCTC AGGCACCTAA ATCCAAACCT 
GAGGAGCAAA AGTCACCGTC TCTGAAGCCA CAACAAGTTG CAAGAAGAGT GAACCCTTCC 
CGGCTGGACA TTGAACTGCT CTCCCCTCTG ACTCCAATAT CCCCTTCTGA GAAGGAGCTG 
AAGAGCTCAG ATGGTGAGAA GAAGGATATC TCCACCAGCT CTGGCTTAGG CCTGAATGGC 
AGTTCCATAA CACGTACTGC CGCCAGTGAA AGCTTTAACA CCTCTGATTC AGCCATGCTT 
ACTCCACCAT CCAGCCCTCC ACCACCTCCT CCCCAAGGGG AAGAGCCAGC TACTCTACGT 
AGAAGCCGTA CTCCTTGCCA GAATGGTCCA GAATCTATCC CAGCCCCTGC CCTAGCACAG 
AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC CGCTGGGAAG AATCTGTACG TAAGTCATTT 
GTAGAAAGTG TAGACGAGAT CCCCTTTGCC GATGATGTGG AGGACACGTA CGACGAAAGA 
ACGGAGAATT CCAGCCTTCA AGAGAGGTTC TTTACCCCAC CAACGAGTCG GGTTAAACCA 
CATAGAATTG GTTTCTCCAA GGAAAATGGA GAGATCCCAT CTTCTTCGGA GAGAGTGCAC 
CACAGAAAAA GGCTCTTGCC CGACGTCACT CAGGAGGCCA AAGAACGGGC AGAGGAGCGG 
ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC CAGGCACTGA AAGACGCCAT AGCACAACAG 
CGGCACAAAA TGAAACAACT AGAACCAGGG GCAAGATCGG CCAAAGCTCT GCCATCTACC 
GTTATGTTCA GCGATTACCC TGCAGTATCT CCTCTCCTGA GAACCCAGAC CGAGACTCCC 
ACCCTGAAGC TGGATACCGA CCAGGAGGTC TTTATAACCC CCCCAGCAGC TCCTGACGGC 
TCCGTCACTT CCTCGGAAGG TTCAGGCGGG AAACCCAAGA AAAGGCCGTC TATATTCTCC 
CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG GAAAGCAGTC GGGCGTCCGA TAAACAGGCG 
ACGGACGAGA GCACCAAACC CAAGTCCTTG TGGAAATCCG TCTTCTCCGG GTATAAGAAG 
GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG GACAAGTCTA CTCCGAGCAC TCCATCCAGC 
AGCACCACCG TGGACTCTGG GAAAGCCAAG GTGGCAAATA TGATCCGCGG AGCAGACCTG 
TCCCTCCGAC GTCACCTGAG CTTCTCGGAG GACTCGGACC TGTCCAGCGA CGATGTCTTG 
GAAAAATCCT CCCAGAAATC TAAGCGAGAG TCGGTGTATG TCCCCCACGC CTTGGCATTC 
AGACGGTCAT ACTCATCAAA GAAAACCTAC ACGGACGAGG AGCTAAGCGC TAAGCTAACA 
AGGCGGGTGC AGAAAGCGGC ACGGAGACAG GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT 
CGGGCACAGA TCATTCAGCG CCAGCTGGAA CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG 
GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC CGGGGAGAAG CAGGGATGGG AAAGAAGGAC 
GACCCGAAAC TCATGCAGGA ATGGTTCCGC CTGGTGCAGG AGAAGAACGC GCTGGTGCGA 
TACGAATCGG AGCTGATGAT ATTTGCCCGC GAGTTGGAGT TGGAGGACAG ACAGAGCCGA 
CTTCAGCAGG AGCTGCGGGA GCGAATGGCA GTGGAAGATC AATTGAAGAC GGAGCAGGAG 
CTGACGGACG AGAAGAAGAT CCTGAACGAG ATGCTGGACG TGGTGGAACA GAGAGATGCT 
CTGGTGGCCC TGTTGGAGGA GCAGAGACTG CGGGAGAAAG AGGAGGACAA AGATCTGGAG 
GAGGTCATGT TATCCAAGGG GTTTAACCTC AACTGGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814837.1
CDS	396..6437
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_012959383.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959383.2

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGGGCTC ATTTAGGAAA GAGTTCCCCC AGAACCTGGG AGGGAGCGAC 
GTCTGCTATT TCTGCCGCCG GAGGGTTTAC GTCATGGAGC GGCTCAGCGC CGAGGGCAAG 
TTCTTCCACC GGAGCTGCTT TAAGTGTGAC TACTGTAGCA CAACGCTGCG CCTCTCATGT 
TATGCCTATG ACCTTGAGGA TGGAAAATTT TACTGCAAAC CTCACTACTG TTACCGCCGG 
TCCGGATACA GCCAGAGGAA GAGGCCGGCC ATGCCTGCAC TTTCGGGAAA AGAAGTCACA 
AGGACTGTGG AAGACACCCC CGACGCGGAA GACCAAGGAC GAGCCCCGCT GCTGCCCGCC 
CCCGCTGCTG AGAAGACCCC AGGCCCCAGT GTGAACGGCG TGGAAGATCA CAGCGCCGCC 
AAGAGGCTGA GAGCCACCCC AGAGCGCATT GAGCTTGAGA ATTACCGCGT CTCCCTGCAG 
AAGGAGGAGC TGGAGGAGGT GCCCGAGGAA ACCCTGGCGG AGCATAATCT GAGCAGCCTA 
TTGGATAAAG GGGAGGTGGA AGAGGTGGCC AGCAGCAGTT CCGAGTCCGA GATGGAGGAG 
GAGGAGGACG AAGAAGAAGA GGAGGAGGAG CCAGGAGAGT GCCTTCCCCC CTCAGATCTG 
GGTGGGGTGC CGTGGAAAGA GGCCGTCAAG ATCCACGCCA AGTTAAAGGG TCAGGACATA 
GATGTGGAGG TTGTGGGAAC CCAGGGGGCT GGTGGCAGTG AAGAGGAGGA GGAGGAGGAG 
GAAGAAGAGG ACGAAGAGGA GGAAGATGAA GAGGTATCAA GTGAAGCCGG GAACTTCAGA 
CTCCAGCAAG CCCTCTCCCC TGTTGACCCC ACGGAAATAA AGGCAGACGT GCGCTGGACA 
CACGTCCGCG AGGTTAAGGA AGAGCGGAGA GGGTCTAAGG AAGAGAGGGG AGAAGTGAGC 
ATGTCCTCTC CTTCCAAAGC GCGTCATCTC CATTCCCACC GTCACGACCC AGTGAGAGCG 
TGGCTGGAGT CCGTATCAGG AGCTACTGGC GAGTATGAAG AAGGTGAGGA GGAGGAGCTG 
GGTACTTGCC TGGCGCAGGC TGAAGCAGCT GGTGATGGGG ACACCTCCGC AGACCTTGGT 
GATGATGATG ACATCCCGTC CGACGCCGAG GCCGAAGAAC GGCTCCAGCA GTCTCAGAGG 
TTTAAATTGA AGCTGGATGT AGCCCAAGAG CCTAAGGTGG AGCAACCCGT CCTGCCAGCA 
ATTGCAGATT CTGAGAGCGT CCCCAGCCCA GTCTCTCTGG CACCGAAAGC CCCCGTGCTG 
TCTCCAAGAA GGACACTCAA AGCGGAAGCA GAAAAGGTTC CTGTGATGTC TCCGAAGGCA 
GTGACCTCTC CTGGGGGTCG GTTCTTTGCG GATCCGTATC TGCCATTGGA CAGCAGCAAC 
AAAGCCTTAG TGGAAGCAAA AACTCCAACT CCTCTATCTC CTGTTTGCTC CCAACCAGTC 
CCATCGCAAG AACCTGTTAT CCCTACATCT CCTGTGAAGT CTCCCTCAGT CCCAGCAAAC 
ACGCGCCCAC TTATATCCCC ATCAGCTCCT ATTTGTTCCC AGCCACTGCC GTCCTCAGAG 
GGTACCCTAA ATGCTACAGA CAGGCCCCCC GTGCGTCAGC AGCCATCCCC TCTAATCCCC 
TCTACTTCTT TAACCAAACT GCCTTTTACT GGTCACGTCA TGGGATCTGA ACAATTAGTT 
AGCCCTAATG CAAAAGAGGT CCTGAAACGT ATTGACCTTA TCGAAGAGTT CTGGACAAAA 
AGCGCCGAGA TCAGGCGCAG CTTAGGGCTG GCCCCCGTGG ACAGAGATCG GGTACATGAG 
ATCAGTTTCC CTGCCCTAGC TTCAACTCCT CTTCAGCCTC AGGCACCTAA ATCCAAACCT 
GAGGAGCAAA AGTCACCGTC TCTGAAGCCA CAACAAGTTG CAAGAAGAGT GAACCCTTCC 
CGGCTGGACA TTGAACTGCT CTCCCCTCTG ACTCCAATAT CCCCTTCTGA GAAGGAGCTG 
AAGAGCTCAG ATGGTGAGAA GAAGGATATC TCCACCAGCT CTGGCTTAGG CCTGAATGGC 
AGTTCCATAA CACGTACTGC CGCCAGTGAA AGCTTTAACA CCTCTGATTC AGCCATGCTT 
ACTCCACCAT CCAGCCCTCC ACCACCTCCT CCCCAAGGGG AAGAGCCAGC TACTCTACGT 
AGAAGCCGTA CTCCTTGCCA GAATGGTCCA GAATCTATCC CAGCCCCTGC CCTAGCACAG 
AGCAAGCCCC CTGAGCCCAA AAAGTCCCCC CGCTGGGAAG AATCTGTACG TAAGTCATTT 
GTAGAAAGTG TAGACGAGAT CCCCTTTGCC GATGATGTGG AGGACACGTA CGACGAAAGA 
ACGGAGAATT CCAGCCTTCA AGAGAGGTTC TTTACCCCAC CAACGAGTCG GGTTAAACCA 
CATAGAATTG GTTTCTCCAA GGAAAATGGA GAGATCCCAT CTTCTTCGGA GAGAGTGCAC 
CACAGAAAAA GGCTCTTGCC CGACGTCACT CAGGAGGCCA AAGAACGGGC AGAGGAGCGG 
ATGAAAGCCC GGGAGAAGTC TGTGAAGAGC CAGGCACTGA AAGACGCCAT AGCACAACAG 
CGGCACAAAA TGAAACAACT AGAACCAGGG GCAAGATCGG CCAAAGCTCT GCCATCTACC 
GTTATGTTCA GCGATTACCC TGCAGTATCT CCTCTCCTGA GAACCCAGAC CGAGACTCCC 
ACCCTGAAGC TGGATACCGA CCAGGAGGTC TTTATAACCC CCCCAGCAGC TCCTGACGGC 
TCCGTCACTT CCTCGGAAGG TTCAGGCGGG AAACCCAAGA AAAGGCCGTC TATATTCTCC 
CCGAGGAAGA AAGAGAAAAA GTCCAAGGGG GAAAGCAGTC GGGCGTCCGA TAAACAGGCG 
ACGGACGAGA GCACCAAACC CAAGTCCTTG TGGAAATCCG TCTTCTCCGG GTATAAGAAG 
GAAAAGAAGA AGAAGCAGCA GCAGGCGGAG GACAAGTCTA CTCCGAGCAC TCCATCCAGC 
AGCACCACCG TGGACTCTGG GAAAGCCAAG GTGGCAAATA TGATCCGCGG AGCAGACCTG 
TCCCTCCGAC GTCACCTGAG CTTCTCGGAG GACTCGGACC TGTCCAGCGA CGATGTCTTG 
GAAAAATCCT CCCAGAAATC TAAGCGAGAG TCGGTGTATG TCCCCCACGC CTTGGCATTC 
AGACGGTCAT ACTCATCAAA GAAAACCTAC ACGGACGAGG AGCTAAGCGC TAAGCTAACA 
AGGCGGGTGC AGAAAGCGGC ACGGAGACAG GCCAAGCAGG AGGAGCTAAA GCGGCTGCAT 
CGGGCACAGA TCATTCAGCG CCAGCTGGAA CAAGTGGAGG AAAAGCAGCG GCAGCTGGAG 
GAGCGAGGGG TGGCTGTGGA GAAAGCTCTC CGGGGAGAAG CAGGGATGGG AAAGAAGGAC 
GACCCGAAAC TCATGCAGGA ATGGTTCCGC CTGGTGCAGG AGAAGAACGC GCTGGTGCGA 
TACGAATCGG AGCTGATGAT ATTTGCCCGC GAGTTGGAGT TGGAGGACAG ACAGAGCCGA 
CTTCAGCAGG AGCTGCGGGA GCGAATGGCA GTGGAAGATC AATTGAAGAC GGAGCAGGAG 
CTGACGGACG AGAAGAAGAT CCTGAACGAG ATGCTGGACG TGGTGGAACA GAGAGATGCT 
CTGGTGGCCC TGTTGGAGGA GCAGAGACTG CGGGAGAAAG AGGAGGACAA AGATCTGGAG 
GAGGTCATGT TATCCAAGGG GTTTAACCTC AACTGGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3 ( XM_018089442.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944931.1 Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X7, mRNA.

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CloneID	OXa40692
Clone ID Related Accession (Same CDS sequence)	XM_018089442.1
Accession Version	XM_018089442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG GGCTCATTTA GGAAAGAGTT CCCCCAGAAC 
CTGGGAGGGA GCGACGTCTG CTATTTCTGC CGCCGGAGGG TTTACGTCAT GGAGCGGCTC 
AGCGCCGAGG GCAAGTTCTT CCACCGGAGC TGCTTTAAGT GTGACTACTG TAGCACAACG 
CTGCGCCTCT CATGTTATGC CTATGACCTT GAGGATGGAA AATTTTACTG CAAACCTCAC 
TACTGTTACC GCCGGTCCGG ATACAGCCAG AGGAAGAGGC CGGCCATGCC TGCACTTTCG 
GGAAAAGAAG TCACAAGGAC TGTGGAAGAC ACCCCCGACG CGGAAGACCA AGGACGAGCC 
CCGCTGCTGC CCGCCCCCGC TGCTGAGAAG ACCCCAGGCC CCAGTGTGAA CGGCGTGGAA 
GATCACAGCG CCGCCAAGAG GCTGAGAGCC ACCCCAGAGC GCATTGAGCT TGAGAATTAC 
CGCGTCTCCC TGCAGAAGGA GGAGCTGGAG GAGGTGCCCG AGGAAACCCT GGCGGAGCAT 
AATCTGAGCA GCCTATTGGA TAAAGGGGAG GTGGAAGAGG TGGCCAGCAG CAGTTCCGAG 
TCCGAGATGG AGGAGGAGGA GGACGAAGAA GAAGAGGAGG AGGAGCCAGG AGAGTGCCTT 
CCCCCCTCAG ATCTGGGTGG GGTGCCGTGG AAAGAGGCCG TCAAGATCCA CGCCAAGTTA 
AAGGGTCAGG ACATAGATGT GGAGGTTGTG GGAACCCAGG GGGCTGGTGG CAGTGAAGAG 
GAGGAGGAGG AGGAGGAAGA AGAGGACGAA GAGGAGGAAG ATGAAGAGGT ATCAAGTGAA 
GAGGGTGAGT ACTGCCCATG GGAGAGAGAG CTTCAGCAGG GCCTGTGGAT ACAGCGTATC 
TCTGATGAAG ATACAGTTGG TATATTTAAA GCCGGGAACT TCAGACTCCA GCAAGCCCTC 
TCCCCTGTTG ACCCCACGGA AATAAAGGCA GACGTGCGCT GGACACACGT CCGCGAGGTT 
AAGGAAGAGC GGAGAGGGTC TAAGGAAGAG AGGGGAGAAG TGAGCATGTC CTCTCCTTCC 
AAAGCGCGTC ATCTCCATTC CCACCGTCAC GACCCAGTGA GAGCGTGGCT GGAGTCCGTA 
TCAGGAGCTA CTGGCGAGTA TGAAGAAGGT GAGGAGGAGG AGCTGGGTAC TTGCCTGGCG 
CAGGCTGAAG CAGCTGGTGA TGGGGACACC TCCGCAGACC TTGGTGATGA TGATGACATC 
CCGTCCGACG CCGAGGCCGA AGAACGGCTC CAGCAGTCTC AGAGGTTTAA ATTGAAGCTG 
GATGTAGCCC AAGAGCCTAA GGTGGAGCAA CCCGTCCTGC CAGCAATTGC AGATTCTGAG 
AGCGTCCCCA GCCCAGTCTC TCTGGCACCG AAAGCCCCCG TGCTGTCTCC AAGAAGGACA 
CTCAAAGCGG AAGCAGAAAA GGTTCCTGTG ATGTCTCCGA AGGCAGTGAC CTCTCCTGGG 
GGTCGGTTCT TTGCGGATCC GTATCTGCCA TTGGACAGCA GCAACAAAGC CTTAGTGGAA 
GCAAAAACTC CAACTCCTCT ATCTCCTGTT TGCTCCCAAC CAGTCCCATC GCAAGAACCT 
GTTATCCCTA CATCTCCTGT GAAGTCTCCC TCAGTCCCAG CAAACACGCG CCCACTTATA 
TCCCCATCAG CTCCTATTTG TTCCCAGCCA CTGCCGTCCT CAGAGGGTAC CCTAAATGCT 
ACAGACAGGC CCCCCGTGCG TCAGCAGCCA TCCCCTCTAA TCCCCTCTAC TTCTTTAACC 
AAACTGCCTT TTACTGGTCA CGTCATGGGA TCTGAACAAT TAGTTAGCCC TAATGCAAAA 
GAGGTCCTGA AACGTATTGA CCTTATCGAA GAGTTCTGGA CAAAAAGCGC CGAGATCAGG 
CGCAGCTTAG GGCTGGCCCC CGTGGACAGA GATCGGGTAC ATGAGATCAG TTTCCCTGCC 
CTAGCTTCAA CTCCTCTTCA GCCTCAGGCA CCTAAATCCA AACCTGAGGA GCAAAAGTCA 
CCGTCTCTGA AGCCACAACA AGTTGCAAGA AGAGTGAACC CTTCCCGGCT GGACATTGAA 
CTGCTCTCCC CTCTGACTCC AATATCCCCT TCTGAGAAGG AGCTGAAGAG CTCAGATGGT 
GAGAAGAAGG ATATCTCCAC CAGCTCTGGC TTAGGCCTGA ATGGCAGTTC CATAACACGT 
ACTGCCGCCA GTGAAAGCTT TAACACCTCT GATTCAGCCA TGCTTACTCC ACCATCCAGC 
CCTCCACCAC CTCCTCCCCA AGGGGAAGAG CCAGCTACTC TACGTAGAAG CCGTACTCCT 
TGCCAGAATG GTCCAGAATC TATCCCAGCC CCTGCCCTAG CACAGAGCAA GCCCCCTGAG 
CCCAAAAAGT CCCCCCGCTG GGAAGAATCT GTACGTAAGT CATTTGTAGA AAGTGTAGAC 
GAGATCCCCT TTGCCGATGA TGTGGAGGAC ACGTACGACG AAAGAACGGA GAATTCCAGC 
CTTCAAGAGA GGTTCTTTAC CCCACCAACG AGTCGGGTTA AACCACATAG AATTGGTTTC 
TCCAAGGAAA ATGGAGAGAT CCCATCTTCT TCGGAGAGAG TGCACCACAG AAAAAGGCTC 
TTGCCCGACG TCACTCAGGA GGCCAAAGAA CGGGCAGAGG AGCGGATGAA AGCCCGGGAG 
AAGTCTGTGA AGAGCCAGGC ACTGAAAGAC GCCATAGCAC AACAGCGGCA CAAAATGAAA 
CAACTAGAAC CAGGGGCAAG ATCGGCCAAA GCTCTGCCAT CTACCGTTAT GTTCAGCGAT 
TACCCTGCAG TATCTCCTCT CCTGAGAACC CAGACCGAGA CTCCCACCCT GAAGCTGGAT 
ACCGACCAGG AGGTCTTTAT AACCCCCCCA GCAGCTCCTG ACGGCTCCGT CACTTCCTCG 
GAAGGTTCAG GCGGGAAACC CAAGAAAAGG CCGTCTATAT TCTCCCCGAG GAAGAAAGAG 
AAAAAGTCCA AGGGGGAAAG CAGTCGGGCG TCCGATAAAC AGGCGACGGA CGAGAGCACC 
AAACCCAAGT CCTTGTGGAA ATCCGTCTTC TCCGGGTATA AGAAGGAAAA GAAGAAGAAG 
CAGCAGCAGG CGGAGGACAA GTCTACTCCG AGCACTCCAT CCAGCAGCAC CACCGTGGAC 
TCTGGGAAAG CCAAGGTGGC AAATATGATC CGCGGAGCAG ACCTGTCCCT CCGACGTCAC 
CTGAGCTTCT CGGAGGACTC GGACCTGTCC AGCGACGATG TCTTGGAAAA ATCCTCCCAG 
AAATCTAAGC GAGAGTCGGT GTATGTCCCC CACGCCTTGG CATTCAGACG GTCATACTCA 
TCAAAGAAAA CCTACACGGA CGAGGAGCTA AGCGCTAAGC TAACAAGGCG GGTGCAGAAA 
GCGGCACGGA GACAGGCCAA GCAGGAGGAG CTAAAGCGGC TGCATCGGGC ACAGATCATT 
CAGCGCCAGC TGGAACAAGT GGAGGAAAAG CAGCGGCAGC TGGAGGAGCG AGGGGTGGCT 
GTGGAGAAAG CTCTCCGGGG AGAAGCAGAC TATTGGGGAG AGTCTTATTA CAGTGACCTC 
ATCGATTTGC ACCTGGGTGT TGATCCCGTT GCCGGGACTC CGCGCCGTAG ACCTCTCTCC 
TTTTGCCCCT GCTGTGCCCA CGAAGGGATG GGAAAGAAGG ACGACCCGAA ACTCATGCAG 
GAATGGTTCC GCCTGGTGCA GGAGAAGAAC GCGCTGGTGC GATACGAATC GGAGCTGATG 
ATATTTGCCC GCGAGTTGGA GTTGGAGGAC AGACAGAGCC GACTTCAGCA GGAGCTGCGG 
GAGCGAATGG CAGTGGAAGA TCAATTGAAG ACGGAGCAGG AGCTGACGGA CGAGAAGAAG 
ATCCTGAACG AGATGCTGGA CGTGGTGGAA CAGAGAGATG CTCTGGTGGC CCTGTTGGAG 
GAGCAGAGAC TGCGGGAGAA AGAGGAGGAC AAAGATCTGG AGGAGGTCAT GTTATCCAAG 
GGGTTTAACC TCAACTGGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944931.1
CDS	398..6721
Translation	MEESKNDKANLSHALFDRFVQATTCKGTLKSFQELCDHLDLKPKDYRTFYHKLKSKLNYWKAKALWSKLDKRGAHKDYKKGKACINTKCLIIGAGPCGLRTAIELCFLGAKVVVVEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVCLILGIEIHVNVEFQGLIEPPEDQENQRSGWRAKVYPKGHCVSDYEFDVIIGADGRRNTLAGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQDLREATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILQDYADTELLLSRANVDQEALLNYATEAARFSTNQQLPALDFAINHYGQPDVAMFDFTCMYASENAALVRERNNQRLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSQGASPMEVLAERESIYRLLPQTTPENVSKNFSQYSVDPTTRYPNVNLQFLRPSQVRHLYNTSELKDPNIEIGNAVNSRTPVLGRNESVARTGKLLGWCQKQMEGYPGVNVTDLTMSWKSGLALCAIIHRYRPELINFESLDEQDVEKNNQLAFDIAERELGISPIMTGKEMASVGEPDKLSMVVYLSQFYEMFKESDAEDANQYADEKAALLASTKSPISFLSKLGQTISRKRTPKDKKEKDSDASGKRRKTSQSEDEEAPRTQREEKPASVSAVAERRNETASAAGQNKVKSMATQLLAKFEENAPAPNAGLRKQRPTLPYQERLQNQASQRQTEPQWKQGSFRKEFPQNLGGSDVCYFCRRRVYVMERLSAEGKFFHRSCFKCDYCSTTLRLSCYAYDLEDGKFYCKPHYCYRRSGYSQRKRPAMPALSGKEVTRTVEDTPDAEDQGRAPLLPAPAAEKTPGPSVNGVEDHSAAKRLRATPERIELENYRVSLQKEELEEVPEETLAEHNLSSLLDKGEVEEVASSSSESEMEEEEDEEEEEEEPGECLPPSDLGGVPWKEAVKIHAKLKGQDIDVEVVGTQGAGGSEEEEEEEEEEDEEEEDEEVSSEEGEYCPWERELQQGLWIQRISDEDTVGIFKAGNFRLQQALSPVDPTEIKADVRWTHVREVKEERRGSKEERGEVSMSSPSKARHLHSHRHDPVRAWLESVSGATGEYEEGEEEELGTCLAQAEAAGDGDTSADLGDDDDIPSDAEAEERLQQSQRFKLKLDVAQEPKVEQPVLPAIADSESVPSPVSLAPKAPVLSPRRTLKAEAEKVPVMSPKAVTSPGGRFFADPYLPLDSSNKALVEAKTPTPLSPVCSQPVPSQEPVIPTSPVKSPSVPANTRPLISPSAPICSQPLPSSEGTLNATDRPPVRQQPSPLIPSTSLTKLPFTGHVMGSEQLVSPNAKEVLKRIDLIEEFWTKSAEIRRSLGLAPVDRDRVHEISFPALASTPLQPQAPKSKPEEQKSPSLKPQQVARRVNPSRLDIELLSPLTPISPSEKELKSSDGEKKDISTSSGLGLNGSSITRTAASESFNTSDSAMLTPPSSPPPPPPQGEEPATLRRSRTPCQNGPESIPAPALAQSKPPEPKKSPRWEESVRKSFVESVDEIPFADDVEDTYDERTENSSLQERFFTPPTSRVKPHRIGFSKENGEIPSSSERVHHRKRLLPDVTQEAKERAEERMKAREKSVKSQALKDAIAQQRHKMKQLEPGARSAKALPSTVMFSDYPAVSPLLRTQTETPTLKLDTDQEVFITPPAAPDGSVTSSEGSGGKPKKRPSIFSPRKKEKKSKGESSRASDKQATDESTKPKSLWKSVFSGYKKEKKKKQQQAEDKSTPSTPSSSTTVDSGKAKVANMIRGADLSLRRHLSFSEDSDLSSDDVLEKSSQKSKRESVYVPHALAFRRSYSSKKTYTDEELSAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYSDLIDLHLGVDPVAGTPRRRPLSFCPCCAHEGMGKKDDPKLMQEWFRLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVEDQLKTEQELTDEKKILNEMLDVVEQRDALVALLEEQRLREKEEDKDLEEVMLSKGFNLNWS

Target ORF information:

RefSeq Version	XM_018089442.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis microtubule associated monooxygenase, calponin and LIM domain containing 3 (mical3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089442.1

ORF Insert Sequence:

ATGGAAGAAA GTAAGAATGA TAAGGCCAAC CTGTCGCACG CGTTGTTTGA CCGCTTTGTC 
CAGGCCACCA CGTGCAAGGG AACTTTGAAG AGTTTCCAGG AGCTGTGCGA CCATTTGGAT 
TTGAAGCCCA AAGACTACCG GACCTTCTAT CATAAACTCA AGTCCAAGCT CAACTATTGG 
AAGGCCAAGG CGCTATGGTC CAAACTGGAC AAGAGAGGGG CTCACAAGGA TTACAAGAAG 
GGGAAAGCAT GCATCAACAC CAAGTGTCTG ATCATCGGGG CGGGACCCTG CGGCCTACGG 
ACAGCGATAG AACTCTGCTT CCTGGGGGCC AAAGTAGTCG TTGTGGAGAA GAGAGACGCC 
TTTTCTCGGA ACAATGTTTT GCACCTTTGG CCCTTCACTA TCCACGACCT GCGTGGGCTG 
GGGGCCAAGA AATTCTACGG GAAGTTCTGC GCCGGAGCCA TTGATCATAT CAGTATCCGG 
CAGCTGCAGC TTATTTTGCT CAAAGTCTGT CTGATCCTTG GGATCGAGAT TCACGTTAAT 
GTCGAGTTCC AAGGTTTGAT CGAACCTCCA GAAGACCAGG AGAATCAGCG GAGCGGCTGG 
CGAGCAAAGG TTTACCCAAA AGGACACTGT GTGTCTGACT ATGAATTCGA TGTCATTATT 
GGTGCAGACG GAAGGCGAAA CACGCTGGCA GGTTTCCGGC GGAAAGAGTT TCGTGGTAAG 
TTGGCGATTG CAATCACGGC CAATTTCATC AACCGCAACA CAACAGCCGA AGCGAAGGTG 
GAGGAGATCA GCGGCGTGGC CTTCATTTTC AACCAGAAGT TCTTTCAGGA TCTACGAGAG 
GCGACAGGGA TCGATCTGGA GAACATTGTT TATTATAAAG ACGACACTCA CTACTTTGTC 
ATGACGGCAA AGAAACAGAG CTTGCTGGAG AAGGGAGTGA TCTTACAGGA TTATGCCGAT 
ACGGAGCTGT TACTTTCCCG TGCCAATGTG GACCAAGAAG CCCTCTTGAA CTATGCCACA 
GAAGCTGCCA GGTTCTCAAC CAATCAGCAG CTGCCCGCGC TGGACTTTGC TATAAATCAC 
TATGGGCAGC CGGATGTGGC CATGTTTGAC TTTACCTGTA TGTACGCGTC GGAGAATGCC 
GCCCTTGTGC GGGAAAGGAA TAACCAGAGG CTGCTGGTGG CATTGGTTGG AGACAGCCTT 
CTGGAGCCCT TCTGGCCTAT GGGAACCGGC ATTGCACGCG GGTTTCTTGC TGCTATGGAT 
TCCGCATGGA TGGTACGAAG CTGGTCTCAA GGTGCCAGCC CTATGGAGGT GCTAGCAGAA 
AGAGAGAGTA TATACAGGCT CCTCCCTCAG ACTACTCCTG AAAATGTGAG CAAGAACTTC 
AGCCAGTACA GTGTCGACCC AACTACTCGG TATCCCAATG TCAACTTGCA GTTCCTCCGA 
CCCAGTCAAG TACGTCACCT GTACAATACC AGTGAGTTGA AGGATCCAAA TATAGAGATT 
GGGAACGCTG TGAACTCAAG GACACCAGTT CTGGGCCGAA ATGAGTCAGT CGCCCGCACC 
GGTAAGCTGC TTGGATGGTG CCAAAAGCAG ATGGAGGGTT ACCCTGGAGT CAATGTGACC 
GACCTTACCA TGTCTTGGAA GAGTGGCCTG GCGTTATGTG CTATTATCCA TCGGTACCGC 
CCTGAGCTCA TAAACTTTGA ATCTCTGGAT GAGCAGGACG TGGAGAAGAA TAACCAGCTG 
GCCTTTGATA TTGCTGAGCG GGAGCTGGGC ATCTCGCCCA TCATGACCGG AAAGGAAATG 
GCTTCTGTGG GGGAACCAGA CAAGCTGTCC ATGGTCGTGT ATCTCAGCCA GTTCTACGAG 
ATGTTCAAGG AGTCTGACGC AGAGGATGCC AACCAATATG CCGATGAGAA GGCCGCCCTG 
CTTGCCAGCA CCAAGTCTCC CATCTCCTTC CTCAGCAAAT TGGGGCAGAC TATCTCTCGG 
AAACGCACTC CCAAGGACAA AAAGGAAAAG GACTCTGATG CCTCAGGAAA GCGAAGGAAA 
ACAAGCCAGT CCGAGGATGA GGAAGCTCCC CGAACGCAGC GGGAGGAAAA GCCGGCATCG 
GTGAGCGCCG TGGCCGAGAG GCGGAATGAG ACTGCGTCTG CCGCCGGCCA AAATAAAGTC 
AAATCAATGG CCACTCAACT CCTGGCAAAA TTTGAGGAGA ACGCCCCGGC CCCCAATGCA 
GGACTGCGCA AACAGAGACC CACCCTTCCC TATCAGGAGC GCTTGCAAAA CCAGGCTTCT 
CAGAGGCAGA CTGAGCCTCA GTGGAAACAG GGCTCATTTA GGAAAGAGTT CCCCCAGAAC 
CTGGGAGGGA GCGACGTCTG CTATTTCTGC CGCCGGAGGG TTTACGTCAT GGAGCGGCTC 
AGCGCCGAGG GCAAGTTCTT CCACCGGAGC TGCTTTAAGT GTGACTACTG TAGCACAACG 
CTGCGCCTCT CATGTTATGC CTATGACCTT GAGGATGGAA AATTTTACTG CAAACCTCAC 
TACTGTTACC GCCGGTCCGG ATACAGCCAG AGGAAGAGGC CGGCCATGCC TGCACTTTCG 
GGAAAAGAAG TCACAAGGAC TGTGGAAGAC ACCCCCGACG CGGAAGACCA AGGACGAGCC 
CCGCTGCTGC CCGCCCCCGC TGCTGAGAAG ACCCCAGGCC CCAGTGTGAA CGGCGTGGAA 
GATCACAGCG CCGCCAAGAG GCTGAGAGCC ACCCCAGAGC GCATTGAGCT TGAGAATTAC 
CGCGTCTCCC TGCAGAAGGA GGAGCTGGAG GAGGTGCCCG AGGAAACCCT GGCGGAGCAT 
AATCTGAGCA GCCTATTGGA TAAAGGGGAG GTGGAAGAGG TGGCCAGCAG CAGTTCCGAG 
TCCGAGATGG AGGAGGAGGA GGACGAAGAA GAAGAGGAGG AGGAGCCAGG AGAGTGCCTT 
CCCCCCTCAG ATCTGGGTGG GGTGCCGTGG AAAGAGGCCG TCAAGATCCA CGCCAAGTTA 
AAGGGTCAGG ACATAGATGT GGAGGTTGTG GGAACCCAGG GGGCTGGTGG CAGTGAAGAG 
GAGGAGGAGG AGGAGGAAGA AGAGGACGAA GAGGAGGAAG ATGAAGAGGT ATCAAGTGAA 
GAGGGTGAGT ACTGCCCATG GGAGAGAGAG CTTCAGCAGG GCCTGTGGAT ACAGCGTATC 
TCTGATGAAG ATACAGTTGG TATATTTAAA GCCGGGAACT TCAGACTCCA GCAAGCCCTC 
TCCCCTGTTG ACCCCACGGA AATAAAGGCA GACGTGCGCT GGACACACGT CCGCGAGGTT 
AAGGAAGAGC GGAGAGGGTC TAAGGAAGAG AGGGGAGAAG TGAGCATGTC CTCTCCTTCC 
AAAGCGCGTC ATCTCCATTC CCACCGTCAC GACCCAGTGA GAGCGTGGCT GGAGTCCGTA 
TCAGGAGCTA CTGGCGAGTA TGAAGAAGGT GAGGAGGAGG AGCTGGGTAC TTGCCTGGCG 
CAGGCTGAAG CAGCTGGTGA TGGGGACACC TCCGCAGACC TTGGTGATGA TGATGACATC 
CCGTCCGACG CCGAGGCCGA AGAACGGCTC CAGCAGTCTC AGAGGTTTAA ATTGAAGCTG 
GATGTAGCCC AAGAGCCTAA GGTGGAGCAA CCCGTCCTGC CAGCAATTGC AGATTCTGAG 
AGCGTCCCCA GCCCAGTCTC TCTGGCACCG AAAGCCCCCG TGCTGTCTCC AAGAAGGACA 
CTCAAAGCGG AAGCAGAAAA GGTTCCTGTG ATGTCTCCGA AGGCAGTGAC CTCTCCTGGG 
GGTCGGTTCT TTGCGGATCC GTATCTGCCA TTGGACAGCA GCAACAAAGC CTTAGTGGAA 
GCAAAAACTC CAACTCCTCT ATCTCCTGTT TGCTCCCAAC CAGTCCCATC GCAAGAACCT 
GTTATCCCTA CATCTCCTGT GAAGTCTCCC TCAGTCCCAG CAAACACGCG CCCACTTATA 
TCCCCATCAG CTCCTATTTG TTCCCAGCCA CTGCCGTCCT CAGAGGGTAC CCTAAATGCT 
ACAGACAGGC CCCCCGTGCG TCAGCAGCCA TCCCCTCTAA TCCCCTCTAC TTCTTTAACC 
AAACTGCCTT TTACTGGTCA CGTCATGGGA TCTGAACAAT TAGTTAGCCC TAATGCAAAA 
GAGGTCCTGA AACGTATTGA CCTTATCGAA GAGTTCTGGA CAAAAAGCGC CGAGATCAGG 
CGCAGCTTAG GGCTGGCCCC CGTGGACAGA GATCGGGTAC ATGAGATCAG TTTCCCTGCC 
CTAGCTTCAA CTCCTCTTCA GCCTCAGGCA CCTAAATCCA AACCTGAGGA GCAAAAGTCA 
CCGTCTCTGA AGCCACAACA AGTTGCAAGA AGAGTGAACC CTTCCCGGCT GGACATTGAA 
CTGCTCTCCC CTCTGACTCC AATATCCCCT TCTGAGAAGG AGCTGAAGAG CTCAGATGGT 
GAGAAGAAGG ATATCTCCAC CAGCTCTGGC TTAGGCCTGA ATGGCAGTTC CATAACACGT 
ACTGCCGCCA GTGAAAGCTT TAACACCTCT GATTCAGCCA TGCTTACTCC ACCATCCAGC 
CCTCCACCAC CTCCTCCCCA AGGGGAAGAG CCAGCTACTC TACGTAGAAG CCGTACTCCT 
TGCCAGAATG GTCCAGAATC TATCCCAGCC CCTGCCCTAG CACAGAGCAA GCCCCCTGAG 
CCCAAAAAGT CCCCCCGCTG GGAAGAATCT GTACGTAAGT CATTTGTAGA AAGTGTAGAC 
GAGATCCCCT TTGCCGATGA TGTGGAGGAC ACGTACGACG AAAGAACGGA GAATTCCAGC 
CTTCAAGAGA GGTTCTTTAC CCCACCAACG AGTCGGGTTA AACCACATAG AATTGGTTTC 
TCCAAGGAAA ATGGAGAGAT CCCATCTTCT TCGGAGAGAG TGCACCACAG AAAAAGGCTC 
TTGCCCGACG TCACTCAGGA GGCCAAAGAA CGGGCAGAGG AGCGGATGAA AGCCCGGGAG 
AAGTCTGTGA AGAGCCAGGC ACTGAAAGAC GCCATAGCAC AACAGCGGCA CAAAATGAAA 
CAACTAGAAC CAGGGGCAAG ATCGGCCAAA GCTCTGCCAT CTACCGTTAT GTTCAGCGAT 
TACCCTGCAG TATCTCCTCT CCTGAGAACC CAGACCGAGA CTCCCACCCT GAAGCTGGAT 
ACCGACCAGG AGGTCTTTAT AACCCCCCCA GCAGCTCCTG ACGGCTCCGT CACTTCCTCG 
GAAGGTTCAG GCGGGAAACC CAAGAAAAGG CCGTCTATAT TCTCCCCGAG GAAGAAAGAG 
AAAAAGTCCA AGGGGGAAAG CAGTCGGGCG TCCGATAAAC AGGCGACGGA CGAGAGCACC 
AAACCCAAGT CCTTGTGGAA ATCCGTCTTC TCCGGGTATA AGAAGGAAAA GAAGAAGAAG 
CAGCAGCAGG CGGAGGACAA GTCTACTCCG AGCACTCCAT CCAGCAGCAC CACCGTGGAC 
TCTGGGAAAG CCAAGGTGGC AAATATGATC CGCGGAGCAG ACCTGTCCCT CCGACGTCAC 
CTGAGCTTCT CGGAGGACTC GGACCTGTCC AGCGACGATG TCTTGGAAAA ATCCTCCCAG 
AAATCTAAGC GAGAGTCGGT GTATGTCCCC CACGCCTTGG CATTCAGACG GTCATACTCA 
TCAAAGAAAA CCTACACGGA CGAGGAGCTA AGCGCTAAGC TAACAAGGCG GGTGCAGAAA 
GCGGCACGGA GACAGGCCAA GCAGGAGGAG CTAAAGCGGC TGCATCGGGC ACAGATCATT 
CAGCGCCAGC TGGAACAAGT GGAGGAAAAG CAGCGGCAGC TGGAGGAGCG AGGGGTGGCT 
GTGGAGAAAG CTCTCCGGGG AGAAGCAGAC TATTGGGGAG AGTCTTATTA CAGTGACCTC 
ATCGATTTGC ACCTGGGTGT TGATCCCGTT GCCGGGACTC CGCGCCGTAG ACCTCTCTCC 
TTTTGCCCCT GCTGTGCCCA CGAAGGGATG GGAAAGAAGG ACGACCCGAA ACTCATGCAG 
GAATGGTTCC GCCTGGTGCA GGAGAAGAAC GCGCTGGTGC GATACGAATC GGAGCTGATG 
ATATTTGCCC GCGAGTTGGA GTTGGAGGAC AGACAGAGCC GACTTCAGCA GGAGCTGCGG 
GAGCGAATGG CAGTGGAAGA TCAATTGAAG ACGGAGCAGG AGCTGACGGA CGAGAAGAAG 
ATCCTGAACG AGATGCTGGA CGTGGTGGAA CAGAGAGATG CTCTGGTGGC CCTGTTGGAG 
GAGCAGAGAC TGCGGGAGAA AGAGGAGGAC AAAGATCTGG AGGAGGTCAT GTTATCCAAG 
GGGTTTAACC TCAACTGGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mical3
Entrez Gene ID	100486205
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms	mical-3
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 3 Names protein-methionine sulfoxide oxidase MICAL3 microtubule associated monoxygenase, calponin and LIM domain containing 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Rattus norvegicus (Norway rat)	Mical3	NP_001178014.1
Pan troglodytes (chimpanzee)	MICAL3	XP_001151830.2
	MICAL3	XP_001103660.2
Canis lupus familiaris (dog)	MICAL3	XP_005637453.1
Bos taurus (cattle)	MICAL3	XP_005207412.1
Danio rerio (zebrafish)	mical3a	XP_005159086.1
Homo sapiens (human)	MICAL3	NP_056056.2
Mus musculus (house mouse)	Mical3	NP_001257404.1
Gallus gallus (chicken)	MICAL3	XP_004938010.1
Xenopus tropicalis (tropical clawed frog)	mical3	XP_004912548.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mical3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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