chaf1a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	chaf1a
Entrez Gene ID	100485264
Full Name	chromatin assembly factor 1 subunit A
Synonyms	caip150
General protein information	Preferred Names chromatin assembly factor 1 subunit A Names chromatin assembly factor 1 subunit A chromatin assembly factor 1, subunit A (p150)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934004.4	XP_002934050.1	chromatin assembly factor 1 subunit A
XM_002934004.5	XP_002934050.1	chromatin assembly factor 1 subunit A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHAF1A	XP_512286.3
	CHAF1A	XP_002801077.1
Bos taurus (cattle)	CHAF1A	NP_001095312.1
Rattus norvegicus (Norway rat)	Chaf1a	XP_343672.2
Gallus gallus (chicken)	CHAF1A	NP_001073220.2
Canis lupus familiaris (dog)	CHAF1A	XP_542156.4
Mus musculus (house mouse)	Chaf1a	NP_038761.1
Xenopus tropicalis (tropical clawed frog)	LOC100485264	XP_002934050.1
Homo sapiens (human)	CHAF1A	NP_005474.2

Gene Ontology

The following chaf1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chaf1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04387	XM_002934004.5 Latest version!	Xenopus tropicalis chromatin assembly factor 1 subunit A (chaf1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa04387	XM_002934004.4	Xenopus tropicalis chromatin assembly factor 1 subunit A (chaf1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chaf1a ( XM_002934004.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934050.1 Xenopus tropicalis chromatin assembly factor 1 subunit A (chaf1a), mRNA.

Price & Availability↑

CloneID	OXa04387
Clone ID Related Accession (Same CDS sequence)	XM_002934004.4 , XM_002934004.5
Accession Version	XM_002934004.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromatin assembly factor 1 subunit A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGA AGGAAGCGAC AGGAGAAGTT ATGAAATCCT CTGCTAAATC TAACTCAAAG 
AAGATGGTAC AAGCTCGTCT GCCTTTCAAA CGCCTGAATC CAGTACCCAA AGATGAAGGC 
TGTCTAGAAG AGAAAAAAGT AAGAATCAGC CAAAATATTT CTCCTCAAAA AATGTTGCAC 
TCTCTCAACT CCTCTATGGA TGATATGGAA AATGATTGTG AAATGGAGAC AGAAGCTACA 
CCAATCCCTA AAGCTGTAAA TGGGAAAGGG CCATTGGACA ACTATATTAG GAAAGCCCCC 
AAAGTCAGCC ATGCTGCCTC TGTCATTACT ATTGATTTGA CAGAAGATTC AAATAGCATT 
TTGAATGACA GCCCATTAAA TGGAGAGTCT CAAACACACC TAGCAAATGG GACAGTCTCA 
CCTGAAAGGA GCACACCCAA TCTTCCATTC AGTACAAAGA AGGAATTTGC TGTTTCTGTG 
GATAACAAGA GTCTCGAGAA CTTGAATTTT TCAGAACTTA AATCAGGTGA GCTGCATCTG 
GCTGCAGCTA CCTGTACTTA TGTACCAAAC TTTTCTCCAG AAAAAGCAGT CAAAGAAGAC 
TGTAACACTT CTGCAGATGA TGACTCTGGT TCTGTCTCCT CCAGCAGTTC TCCCATTTCA 
CTTAGCTCCC CAGATGCACA GACTGGTTCC CAATTCAGGA ATGGAAGCAG CCCATCAACC 
TCCACTACCC CTACTGGCAA AGTTAATGCA AGTAAGACTT CATCAGAAAA GAAGAAAACC 
AAAGGTAAGG AAGAAAAGGG GCAAGCTGAA AAGGAGGAGC GAAAGCGTGC TAGAGAAGAT 
GCAAGGGCTG CAAAAGAACT TGCAAAGAAG AAGAGGGACG ATGAGAGAGA GCAGCGGGAA 
AGGGACAAGA AGGAAAAAAA GGAGAGAGAA GACCGAGAAA AGGCAGAAAA GTTGCGACTA 
AAGGAGGAGA AAAAAAAGGA GAAGCTTGAA GCCCTGGAGG CTAAGCAAGA AGAAAAACGG 
AAAAAAGAAG AGGAAAAGCG GCAAAAAGAA GAGGAAAAGA AACTGAAAGA AGAAGAAAAG 
CGCCTAAAGG CTGAGAAAGC AGAGATCACT AGATTTTTTC AGAAGCCAAA AACACCACAA 
GCTCCTAAGA CATTTGCTAG GTCCTGTGGG AAATTTGCCC CTTTTGAGAT TAAAAAGGAT 
ATGGCATTAG CACCTCTGTG TCGTATTGAT TTTGAACCAG AAGCATCTGA AGAACTGGAT 
AAATTCCTTC AGGAGCAAAA TTCAAGGTCT AACTTCCTTG ATGAAATAAA AAAGAGAAAA 
CCGCGCAAAA TGGGTCAGAC TATAGTGCCA ACCATTAACA GCATCGAGGT AGATGGTGTA 
CAGGTACTTG AAGAGCCAGA TCCTGTACTA GGTTCAAATA TGGTAATTGA AGGGCACGTT 
AAAGACATTG GTGTTCCAGA GCGGATCAAG TTTGGGAGAA TGAAACTGCT ACAGTTTTGT 
GAAAATTACC GGCCTGCCTA CTGGGGCACA TGGAATAGAA GGAGCAGAGT AATCAACCCA 
CGTCAGCCGT GGATTCAGGA CACAGGCATA CTAGATTATG AAGCTGATAG CGATGAAGAG 
TGGGAAGAGG AAGAGCCTGG AGAGTCTTTG TCGCACAGTG AGGGGGAGAA TGAAGATGAT 
GATCCCAAAG AAGAAGACGA GGAAGATGAT GATGGCTTCT TTGTTCCCCA TGGATACTTA 
TCTGATGATG AAGGGGTATC TGATGAGGAA TGCACAGACC CAGAGAACCA AAAAGTTAGA 
CAGAAGCTGA AGGCTAAGGA GTGGGATGAG TTGCAATCAA ACAGCAAGAA GATCCGTGTA 
CTGCAGCCTG TAGTGATTGG ATGTGTATGG TGGGACAGTA ATGCTTCAGA AATTAGACTC 
CTGCAAAAAT TTTCTGCCTG TATTCTGGAG TCACCTGCAG TTGAGGAAGA GCTTGCACAA 
GAGATTAGTT CTGCCCAGAA CCTCAAGGAC AGGCAAATAC TTTCCAAACT TGTCCCTCTA 
CTTCATGGAA ATGTCAATGG AAGTAAGATT ATAATACAAG AATTTCAGGA GTGCTGTCGC 
CGAGGTTTAT TATTGGAGGA TAACACAAGT ACTACCACAG ACAATGAGTC CCCCAGCCCT 
AACTCCACAC CACAAACACC AAACAATGTT CTAGTCCCTT CTAAGGCTCA CTTAAAGCGT 
TTAATCTCTG AAAACTCAGT GTATGAAAAG CGCCCAGACC ACCGTATGTG TTGGTATGTG 
CATTCTGATG TCTTAAAGGT CCTACAACAA GACAATCTTC CTGTACCCTG CCAGTGGATT 
TACATTACTC AAGTTAACTC TGTAGCCAAG GAAGATAATG GTCCCAATGG AGGACCATTG 
CAATCTCTGC CTTCGTCTAA CAAGAGGAAG TCTGCAGGCA GCATGCCTAT CACAAAGTTT 
ATGAAGCGAG CAAGGGACCT TGAAATGGCT GTAAATACAG ATATGGATGG CTTTCAGGCA 
GACAATGAAG AAGAGGATGA TGACTGTATG ATTCTAGAGG ACCAACAAGC TAAAAATGCA 
GAGGACTCCA CCATAAAATG CAAGATAAAC CTGAATGATT CTGCTGTCCT TGCCTCCTGT 
CAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934050.1
CDS	305..2953
Translation	MPGKEATGEVMKSSAKSNSKKMVQARLPFKRLNPVPKDEGCLEEKKVRISQNISPQKMLHSLNSSMDDMENDCEMETEATPIPKAVNGKGPLDNYIRKAPKVSHAASVITIDLTEDSNSILNDSPLNGESQTHLANGTVSPERSTPNLPFSTKKEFAVSVDNKSLENLNFSELKSGELHLAAATCTYVPNFSPEKAVKEDCNTSADDDSGSVSSSSSPISLSSPDAQTGSQFRNGSSPSTSTTPTGKVNASKTSSEKKKTKGKEEKGQAEKEERKRAREDARAAKELAKKKRDDEREQRERDKKEKKEREDREKAEKLRLKEEKKKEKLEALEAKQEEKRKKEEEKRQKEEEKKLKEEEKRLKAEKAEITRFFQKPKTPQAPKTFARSCGKFAPFEIKKDMALAPLCRIDFEPEASEELDKFLQEQNSRSNFLDEIKKRKPRKMGQTIVPTINSIEVDGVQVLEEPDPVLGSNMVIEGHVKDIGVPERIKFGRMKLLQFCENYRPAYWGTWNRRSRVINPRQPWIQDTGILDYEADSDEEWEEEEPGESLSHSEGENEDDDPKEEDEEDDDGFFVPHGYLSDDEGVSDEECTDPENQKVRQKLKAKEWDELQSNSKKIRVLQPVVIGCVWWDSNASEIRLLQKFSACILESPAVEEELAQEISSAQNLKDRQILSKLVPLLHGNVNGSKIIIQEFQECCRRGLLLEDNTSTTTDNESPSPNSTPQTPNNVLVPSKAHLKRLISENSVYEKRPDHRMCWYVHSDVLKVLQQDNLPVPCQWIYITQVNSVAKEDNGPNGGPLQSLPSSNKRKSAGSMPITKFMKRARDLEMAVNTDMDGFQADNEEEDDDCMILEDQQAKNAEDSTIKCKINLNDSAVLASCQD

Target ORF information:

RefSeq Version	XM_002934004.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromatin assembly factor 1 subunit A (chaf1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934004.4

ORF Insert Sequence:

ATGCCTGGGA AGGAAGCGAC AGGAGAAGTT ATGAAATCCT CTGCTAAATC TAACTCAAAG 
AAGATGGTAC AAGCTCGTCT GCCTTTCAAA CGCCTGAATC CAGTACCCAA AGATGAAGGC 
TGTCTAGAAG AGAAAAAAGT AAGAATCAGC CAAAATATTT CTCCTCAAAA AATGTTGCAC 
TCTCTCAACT CCTCTATGGA TGATATGGAA AATGATTGTG AAATGGAGAC AGAAGCTACA 
CCAATCCCTA AAGCTGTAAA TGGGAAAGGG CCATTGGACA ACTATATTAG GAAAGCCCCC 
AAAGTCAGCC ATGCTGCCTC TGTCATTACT ATTGATTTGA CAGAAGATTC AAATAGCATT 
TTGAATGACA GCCCATTAAA TGGAGAGTCT CAAACACACC TAGCAAATGG GACAGTCTCA 
CCTGAAAGGA GCACACCCAA TCTTCCATTC AGTACAAAGA AGGAATTTGC TGTTTCTGTG 
GATAACAAGA GTCTCGAGAA CTTGAATTTT TCAGAACTTA AATCAGGTGA GCTGCATCTG 
GCTGCAGCTA CCTGTACTTA TGTACCAAAC TTTTCTCCAG AAAAAGCAGT CAAAGAAGAC 
TGTAACACTT CTGCAGATGA TGACTCTGGT TCTGTCTCCT CCAGCAGTTC TCCCATTTCA 
CTTAGCTCCC CAGATGCACA GACTGGTTCC CAATTCAGGA ATGGAAGCAG CCCATCAACC 
TCCACTACCC CTACTGGCAA AGTTAATGCA AGTAAGACTT CATCAGAAAA GAAGAAAACC 
AAAGGTAAGG AAGAAAAGGG GCAAGCTGAA AAGGAGGAGC GAAAGCGTGC TAGAGAAGAT 
GCAAGGGCTG CAAAAGAACT TGCAAAGAAG AAGAGGGACG ATGAGAGAGA GCAGCGGGAA 
AGGGACAAGA AGGAAAAAAA GGAGAGAGAA GACCGAGAAA AGGCAGAAAA GTTGCGACTA 
AAGGAGGAGA AAAAAAAGGA GAAGCTTGAA GCCCTGGAGG CTAAGCAAGA AGAAAAACGG 
AAAAAAGAAG AGGAAAAGCG GCAAAAAGAA GAGGAAAAGA AACTGAAAGA AGAAGAAAAG 
CGCCTAAAGG CTGAGAAAGC AGAGATCACT AGATTTTTTC AGAAGCCAAA AACACCACAA 
GCTCCTAAGA CATTTGCTAG GTCCTGTGGG AAATTTGCCC CTTTTGAGAT TAAAAAGGAT 
ATGGCATTAG CACCTCTGTG TCGTATTGAT TTTGAACCAG AAGCATCTGA AGAACTGGAT 
AAATTCCTTC AGGAGCAAAA TTCAAGGTCT AACTTCCTTG ATGAAATAAA AAAGAGAAAA 
CCGCGCAAAA TGGGTCAGAC TATAGTGCCA ACCATTAACA GCATCGAGGT AGATGGTGTA 
CAGGTACTTG AAGAGCCAGA TCCTGTACTA GGTTCAAATA TGGTAATTGA AGGGCACGTT 
AAAGACATTG GTGTTCCAGA GCGGATCAAG TTTGGGAGAA TGAAACTGCT ACAGTTTTGT 
GAAAATTACC GGCCTGCCTA CTGGGGCACA TGGAATAGAA GGAGCAGAGT AATCAACCCA 
CGTCAGCCGT GGATTCAGGA CACAGGCATA CTAGATTATG AAGCTGATAG CGATGAAGAG 
TGGGAAGAGG AAGAGCCTGG AGAGTCTTTG TCGCACAGTG AGGGGGAGAA TGAAGATGAT 
GATCCCAAAG AAGAAGACGA GGAAGATGAT GATGGCTTCT TTGTTCCCCA TGGATACTTA 
TCTGATGATG AAGGGGTATC TGATGAGGAA TGCACAGACC CAGAGAACCA AAAAGTTAGA 
CAGAAGCTGA AGGCTAAGGA GTGGGATGAG TTGCAATCAA ACAGCAAGAA GATCCGTGTA 
CTGCAGCCTG TAGTGATTGG ATGTGTATGG TGGGACAGTA ATGCTTCAGA AATTAGACTC 
CTGCAAAAAT TTTCTGCCTG TATTCTGGAG TCACCTGCAG TTGAGGAAGA GCTTGCACAA 
GAGATTAGTT CTGCCCAGAA CCTCAAGGAC AGGCAAATAC TTTCCAAACT TGTCCCTCTA 
CTTCATGGAA ATGTCAATGG AAGTAAGATT ATAATACAAG AATTTCAGGA GTGCTGTCGC 
CGAGGTTTAT TATTGGAGGA TAACACAAGT ACTACCACAG ACAATGAGTC CCCCAGCCCT 
AACTCCACAC CACAAACACC AAACAATGTT CTAGTCCCTT CTAAGGCTCA CTTAAAGCGT 
TTAATCTCTG AAAACTCAGT GTATGAAAAG CGCCCAGACC ACCGTATGTG TTGGTATGTG 
CATTCTGATG TCTTAAAGGT CCTACAACAA GACAATCTTC CTGTACCCTG CCAGTGGATT 
TACATTACTC AAGTTAACTC TGTAGCCAAG GAAGATAATG GTCCCAATGG AGGACCATTG 
CAATCTCTGC CTTCGTCTAA CAAGAGGAAG TCTGCAGGCA GCATGCCTAT CACAAAGTTT 
ATGAAGCGAG CAAGGGACCT TGAAATGGCT GTAAATACAG ATATGGATGG CTTTCAGGCA 
GACAATGAAG AAGAGGATGA TGACTGTATG ATTCTAGAGG ACCAACAAGC TAAAAATGCA 
GAGGACTCCA CCATAAAATG CAAGATAAAC CTGAATGATT CTGCTGTCCT TGCCTCCTGT 
CAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chaf1a ( XM_002934004.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934050.1 Xenopus tropicalis chromatin assembly factor 1 subunit A (chaf1a), mRNA.

Price & Availability↑

CloneID	OXa04387
Clone ID Related Accession (Same CDS sequence)	XM_002934004.4 , XM_002934004.5
Accession Version	XM_002934004.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromatin assembly factor 1 subunit A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934004.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGA AGGAAGCGAC AGGAGAAGTT ATGAAATCCT CTGCTAAATC TAACTCAAAG 
AAGATGGTAC AAGCTCGTCT GCCTTTCAAA CGCCTGAATC CAGTACCCAA AGATGAAGGC 
TGTCTAGAAG AGAAAAAAGT AAGAATCAGC CAAAATATTT CTCCTCAAAA AATGTTGCAC 
TCTCTCAACT CCTCTATGGA TGATATGGAA AATGATTGTG AAATGGAGAC AGAAGCTACA 
CCAATCCCTA AAGCTGTAAA TGGGAAAGGG CCATTGGACA ACTATATTAG GAAAGCCCCC 
AAAGTCAGCC ATGCTGCCTC TGTCATTACT ATTGATTTGA CAGAAGATTC AAATAGCATT 
TTGAATGACA GCCCATTAAA TGGAGAGTCT CAAACACACC TAGCAAATGG GACAGTCTCA 
CCTGAAAGGA GCACACCCAA TCTTCCATTC AGTACAAAGA AGGAATTTGC TGTTTCTGTG 
GATAACAAGA GTCTCGAGAA CTTGAATTTT TCAGAACTTA AATCAGGTGA GCTGCATCTG 
GCTGCAGCTA CCTGTACTTA TGTACCAAAC TTTTCTCCAG AAAAAGCAGT CAAAGAAGAC 
TGTAACACTT CTGCAGATGA TGACTCTGGT TCTGTCTCCT CCAGCAGTTC TCCCATTTCA 
CTTAGCTCCC CAGATGCACA GACTGGTTCC CAATTCAGGA ATGGAAGCAG CCCATCAACC 
TCCACTACCC CTACTGGCAA AGTTAATGCA AGTAAGACTT CATCAGAAAA GAAGAAAACC 
AAAGGTAAGG AAGAAAAGGG GCAAGCTGAA AAGGAGGAGC GAAAGCGTGC TAGAGAAGAT 
GCAAGGGCTG CAAAAGAACT TGCAAAGAAG AAGAGGGACG ATGAGAGAGA GCAGCGGGAA 
AGGGACAAGA AGGAAAAAAA GGAGAGAGAA GACCGAGAAA AGGCAGAAAA GTTGCGACTA 
AAGGAGGAGA AAAAAAAGGA GAAGCTTGAA GCCCTGGAGG CTAAGCAAGA AGAAAAACGG 
AAAAAAGAAG AGGAAAAGCG GCAAAAAGAA GAGGAAAAGA AACTGAAAGA AGAAGAAAAG 
CGCCTAAAGG CTGAGAAAGC AGAGATCACT AGATTTTTTC AGAAGCCAAA AACACCACAA 
GCTCCTAAGA CATTTGCTAG GTCCTGTGGG AAATTTGCCC CTTTTGAGAT TAAAAAGGAT 
ATGGCATTAG CACCTCTGTG TCGTATTGAT TTTGAACCAG AAGCATCTGA AGAACTGGAT 
AAATTCCTTC AGGAGCAAAA TTCAAGGTCT AACTTCCTTG ATGAAATAAA AAAGAGAAAA 
CCGCGCAAAA TGGGTCAGAC TATAGTGCCA ACCATTAACA GCATCGAGGT AGATGGTGTA 
CAGGTACTTG AAGAGCCAGA TCCTGTACTA GGTTCAAATA TGGTAATTGA AGGGCACGTT 
AAAGACATTG GTGTTCCAGA GCGGATCAAG TTTGGGAGAA TGAAACTGCT ACAGTTTTGT 
GAAAATTACC GGCCTGCCTA CTGGGGCACA TGGAATAGAA GGAGCAGAGT AATCAACCCA 
CGTCAGCCGT GGATTCAGGA CACAGGCATA CTAGATTATG AAGCTGATAG CGATGAAGAG 
TGGGAAGAGG AAGAGCCTGG AGAGTCTTTG TCGCACAGTG AGGGGGAGAA TGAAGATGAT 
GATCCCAAAG AAGAAGACGA GGAAGATGAT GATGGCTTCT TTGTTCCCCA TGGATACTTA 
TCTGATGATG AAGGGGTATC TGATGAGGAA TGCACAGACC CAGAGAACCA AAAAGTTAGA 
CAGAAGCTGA AGGCTAAGGA GTGGGATGAG TTGCAATCAA ACAGCAAGAA GATCCGTGTA 
CTGCAGCCTG TAGTGATTGG ATGTGTATGG TGGGACAGTA ATGCTTCAGA AATTAGACTC 
CTGCAAAAAT TTTCTGCCTG TATTCTGGAG TCACCTGCAG TTGAGGAAGA GCTTGCACAA 
GAGATTAGTT CTGCCCAGAA CCTCAAGGAC AGGCAAATAC TTTCCAAACT TGTCCCTCTA 
CTTCATGGAA ATGTCAATGG AAGTAAGATT ATAATACAAG AATTTCAGGA GTGCTGTCGC 
CGAGGTTTAT TATTGGAGGA TAACACAAGT ACTACCACAG ACAATGAGTC CCCCAGCCCT 
AACTCCACAC CACAAACACC AAACAATGTT CTAGTCCCTT CTAAGGCTCA CTTAAAGCGT 
TTAATCTCTG AAAACTCAGT GTATGAAAAG CGCCCAGACC ACCGTATGTG TTGGTATGTG 
CATTCTGATG TCTTAAAGGT CCTACAACAA GACAATCTTC CTGTACCCTG CCAGTGGATT 
TACATTACTC AAGTTAACTC TGTAGCCAAG GAAGATAATG GTCCCAATGG AGGACCATTG 
CAATCTCTGC CTTCGTCTAA CAAGAGGAAG TCTGCAGGCA GCATGCCTAT CACAAAGTTT 
ATGAAGCGAG CAAGGGACCT TGAAATGGCT GTAAATACAG ATATGGATGG CTTTCAGGCA 
GACAATGAAG AAGAGGATGA TGACTGTATG ATTCTAGAGG ACCAACAAGC TAAAAATGCA 
GAGGACTCCA CCATAAAATG CAAGATAAAC CTGAATGATT CTGCTGTCCT TGCCTCCTGT 
CAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934050.1
CDS	305..2953
Translation	MPGKEATGEVMKSSAKSNSKKMVQARLPFKRLNPVPKDEGCLEEKKVRISQNISPQKMLHSLNSSMDDMENDCEMETEATPIPKAVNGKGPLDNYIRKAPKVSHAASVITIDLTEDSNSILNDSPLNGESQTHLANGTVSPERSTPNLPFSTKKEFAVSVDNKSLENLNFSELKSGELHLAAATCTYVPNFSPEKAVKEDCNTSADDDSGSVSSSSSPISLSSPDAQTGSQFRNGSSPSTSTTPTGKVNASKTSSEKKKTKGKEEKGQAEKEERKRAREDARAAKELAKKKRDDEREQRERDKKEKKEREDREKAEKLRLKEEKKKEKLEALEAKQEEKRKKEEEKRQKEEEKKLKEEEKRLKAEKAEITRFFQKPKTPQAPKTFARSCGKFAPFEIKKDMALAPLCRIDFEPEASEELDKFLQEQNSRSNFLDEIKKRKPRKMGQTIVPTINSIEVDGVQVLEEPDPVLGSNMVIEGHVKDIGVPERIKFGRMKLLQFCENYRPAYWGTWNRRSRVINPRQPWIQDTGILDYEADSDEEWEEEEPGESLSHSEGENEDDDPKEEDEEDDDGFFVPHGYLSDDEGVSDEECTDPENQKVRQKLKAKEWDELQSNSKKIRVLQPVVIGCVWWDSNASEIRLLQKFSACILESPAVEEELAQEISSAQNLKDRQILSKLVPLLHGNVNGSKIIIQEFQECCRRGLLLEDNTSTTTDNESPSPNSTPQTPNNVLVPSKAHLKRLISENSVYEKRPDHRMCWYVHSDVLKVLQQDNLPVPCQWIYITQVNSVAKEDNGPNGGPLQSLPSSNKRKSAGSMPITKFMKRARDLEMAVNTDMDGFQADNEEEDDDCMILEDQQAKNAEDSTIKCKINLNDSAVLASCQD

Target ORF information:

RefSeq Version	XM_002934004.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromatin assembly factor 1 subunit A (chaf1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934004.5

ORF Insert Sequence:

ATGCCTGGGA AGGAAGCGAC AGGAGAAGTT ATGAAATCCT CTGCTAAATC TAACTCAAAG 
AAGATGGTAC AAGCTCGTCT GCCTTTCAAA CGCCTGAATC CAGTACCCAA AGATGAAGGC 
TGTCTAGAAG AGAAAAAAGT AAGAATCAGC CAAAATATTT CTCCTCAAAA AATGTTGCAC 
TCTCTCAACT CCTCTATGGA TGATATGGAA AATGATTGTG AAATGGAGAC AGAAGCTACA 
CCAATCCCTA AAGCTGTAAA TGGGAAAGGG CCATTGGACA ACTATATTAG GAAAGCCCCC 
AAAGTCAGCC ATGCTGCCTC TGTCATTACT ATTGATTTGA CAGAAGATTC AAATAGCATT 
TTGAATGACA GCCCATTAAA TGGAGAGTCT CAAACACACC TAGCAAATGG GACAGTCTCA 
CCTGAAAGGA GCACACCCAA TCTTCCATTC AGTACAAAGA AGGAATTTGC TGTTTCTGTG 
GATAACAAGA GTCTCGAGAA CTTGAATTTT TCAGAACTTA AATCAGGTGA GCTGCATCTG 
GCTGCAGCTA CCTGTACTTA TGTACCAAAC TTTTCTCCAG AAAAAGCAGT CAAAGAAGAC 
TGTAACACTT CTGCAGATGA TGACTCTGGT TCTGTCTCCT CCAGCAGTTC TCCCATTTCA 
CTTAGCTCCC CAGATGCACA GACTGGTTCC CAATTCAGGA ATGGAAGCAG CCCATCAACC 
TCCACTACCC CTACTGGCAA AGTTAATGCA AGTAAGACTT CATCAGAAAA GAAGAAAACC 
AAAGGTAAGG AAGAAAAGGG GCAAGCTGAA AAGGAGGAGC GAAAGCGTGC TAGAGAAGAT 
GCAAGGGCTG CAAAAGAACT TGCAAAGAAG AAGAGGGACG ATGAGAGAGA GCAGCGGGAA 
AGGGACAAGA AGGAAAAAAA GGAGAGAGAA GACCGAGAAA AGGCAGAAAA GTTGCGACTA 
AAGGAGGAGA AAAAAAAGGA GAAGCTTGAA GCCCTGGAGG CTAAGCAAGA AGAAAAACGG 
AAAAAAGAAG AGGAAAAGCG GCAAAAAGAA GAGGAAAAGA AACTGAAAGA AGAAGAAAAG 
CGCCTAAAGG CTGAGAAAGC AGAGATCACT AGATTTTTTC AGAAGCCAAA AACACCACAA 
GCTCCTAAGA CATTTGCTAG GTCCTGTGGG AAATTTGCCC CTTTTGAGAT TAAAAAGGAT 
ATGGCATTAG CACCTCTGTG TCGTATTGAT TTTGAACCAG AAGCATCTGA AGAACTGGAT 
AAATTCCTTC AGGAGCAAAA TTCAAGGTCT AACTTCCTTG ATGAAATAAA AAAGAGAAAA 
CCGCGCAAAA TGGGTCAGAC TATAGTGCCA ACCATTAACA GCATCGAGGT AGATGGTGTA 
CAGGTACTTG AAGAGCCAGA TCCTGTACTA GGTTCAAATA TGGTAATTGA AGGGCACGTT 
AAAGACATTG GTGTTCCAGA GCGGATCAAG TTTGGGAGAA TGAAACTGCT ACAGTTTTGT 
GAAAATTACC GGCCTGCCTA CTGGGGCACA TGGAATAGAA GGAGCAGAGT AATCAACCCA 
CGTCAGCCGT GGATTCAGGA CACAGGCATA CTAGATTATG AAGCTGATAG CGATGAAGAG 
TGGGAAGAGG AAGAGCCTGG AGAGTCTTTG TCGCACAGTG AGGGGGAGAA TGAAGATGAT 
GATCCCAAAG AAGAAGACGA GGAAGATGAT GATGGCTTCT TTGTTCCCCA TGGATACTTA 
TCTGATGATG AAGGGGTATC TGATGAGGAA TGCACAGACC CAGAGAACCA AAAAGTTAGA 
CAGAAGCTGA AGGCTAAGGA GTGGGATGAG TTGCAATCAA ACAGCAAGAA GATCCGTGTA 
CTGCAGCCTG TAGTGATTGG ATGTGTATGG TGGGACAGTA ATGCTTCAGA AATTAGACTC 
CTGCAAAAAT TTTCTGCCTG TATTCTGGAG TCACCTGCAG TTGAGGAAGA GCTTGCACAA 
GAGATTAGTT CTGCCCAGAA CCTCAAGGAC AGGCAAATAC TTTCCAAACT TGTCCCTCTA 
CTTCATGGAA ATGTCAATGG AAGTAAGATT ATAATACAAG AATTTCAGGA GTGCTGTCGC 
CGAGGTTTAT TATTGGAGGA TAACACAAGT ACTACCACAG ACAATGAGTC CCCCAGCCCT 
AACTCCACAC CACAAACACC AAACAATGTT CTAGTCCCTT CTAAGGCTCA CTTAAAGCGT 
TTAATCTCTG AAAACTCAGT GTATGAAAAG CGCCCAGACC ACCGTATGTG TTGGTATGTG 
CATTCTGATG TCTTAAAGGT CCTACAACAA GACAATCTTC CTGTACCCTG CCAGTGGATT 
TACATTACTC AAGTTAACTC TGTAGCCAAG GAAGATAATG GTCCCAATGG AGGACCATTG 
CAATCTCTGC CTTCGTCTAA CAAGAGGAAG TCTGCAGGCA GCATGCCTAT CACAAAGTTT 
ATGAAGCGAG CAAGGGACCT TGAAATGGCT GTAAATACAG ATATGGATGG CTTTCAGGCA 
GACAATGAAG AAGAGGATGA TGACTGTATG ATTCTAGAGG ACCAACAAGC TAAAAATGCA 
GAGGACTCCA CCATAAAATG CAAGATAAAC CTGAATGATT CTGCTGTCCT TGCCTCCTGT 
CAAGACTAA