agap1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004917869.3	XP_004917926.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2
XM_004917870.3	XP_004917927.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X3
XM_004917870.4	XP_004917927.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X3
XM_004917872.4	XP_004917929.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X5
XM_004917868.3	XP_004917925.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X1
XM_004917869.4	XP_004917926.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2
XM_002931998.4	XP_002932044.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X6
XM_004917871.4	XP_004917928.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X4
XM_004917871.3	XP_004917928.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X4
XM_004917872.3	XP_004917929.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X5
XM_004917868.4	XP_004917925.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X1
XM_002931998.5	XP_002932044.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa16170	XM_004917871.3 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa16171	XM_004917872.3 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa16172	XM_002931998.4 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa66205	XM_004917871.4 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa66206	XM_004917872.4 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa66207	XM_002931998.5 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa66202	XM_004917868.4 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa66203	XM_004917869.4 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa66204	XM_004917870.4 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa16167	XM_004917868.3 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa16168	XM_004917869.3 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa16169	XM_004917870.3 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

agap1 ( XM_004917871.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917928.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa16170
Clone ID Related Accession (Same CDS sequence)	XM_004917871.3
Accession Version	XM_004917871.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG TTCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG ATATGGATGC AGGTGGGAGG TTCAAGAAGG AAATTGTAGT AGATGGACAG 
AGCTACCTTC TTCTGATCAG GGATGAAGGG GGACCCCCCG AAGCACAATT TGCCATGTGG 
GTGGATGCAG TTATCTTTGT CTTCAGCCTG GAAGATGAGA TCAGCTTTCA GACAGTCTAT 
CACTACTACA GCCGCCTTGC CAACTACCGC AACACCAGCG AGATCCCTAT GGTGCTGGTA 
GGCACTCAAG ATGCCATTAG CTGCAGTAAC CCCCGTGTAA TTGATGACAG CCGGGCACGA 
AAGCTTTCTA ATGACCTCAA GCGATGCACT TATTATGAGA CCTGCGCCAC CTATGGGCTA 
AATGTGGAGC GTGTTTTCCA AGATGTGGCC CAGAAGATTG TAGCCACCCG TAAGAAACAA 
CAGCTTTCCA TCGGACCCTG CAAGTCCCTG CCAAACTCCC CCAGCCACAC GTCTGTCTGT 
TCTACGCAGG TTTCGGCTGT TCACATTAGT CAGACCAGTA ATGGTGGGGG TAGCCTAAGC 
GACTATTCCT CATCTGTCCC CTCTACACCG AGTACTAGTC AGAAGGAGCT GAGGATCGAC 
GTGCCCCCAG CAGCCAGCAC ACCCACTCCC GTGCGCAAGC AATCAAAACG GCGCTCCAAT 
CTCTTCACGT CTCGCAAAGG GAGCGACCCA GACAAAGATA AGAAAGGTCT AGAGAGCAGA 
GCAGACAGCA TCGGCAGCGG GCGAGCAATA CCAATTAAAC AGGGCATGCT ACTGAAAAGA 
AGCGGAAAGT CTCTTAACAA AGAGTGGAAG AAGAAATACG TAACCTTGTG TGATAACGGA 
GTTCTGACAT ATCATCCCAG CTTACACGAT TACATGCAAA ATGTCCATGG TAAAGAGATC 
GACCTCCTTA GAACTACTGT GAAGGTCCCT GGGAAAAGGC CTCCTCGAGC CACATCCTCT 
TGTGCACCAG TTGCCAGCCC TAAAACCAAT GGTCTAACCA AAGAGGTCAG CGGGCTCCAG 
ATTTCCCCTA ACACAGGGAA CGTGACCAGC AGTACGTCAG TCACCCAAAT GGCCAGTGGT 
CCAAGTGGGA TTAGTTTAGG CTCATTCAGC CGCATGGATG GGATGCACCA GAGGTCATAC 
TCTGTGTCTA GCGCTGACCA GTGGAGCGAG GGAGCCGTCA TCGCCAACTC AGCCATCAGC 
AGCGACACTG GCCTAGGAGA CTCAGTGTGT TCCAGTCCTA GCATCTCCAG CACTACAAGT 
CCCAAGCTAG ATCCGCCACC CTCGCCACAT GCAAACCGCA AGAAGCACCG CCGGAAGAAA 
AGTACCAGTA ACTTTAAGGC TGATGGGCTG TCAAGTACTG CTGAAGCTAA ACGTAAAGCA 
TGGAAACTTA ACCGTGTTGG CAGCCTGCGA AATGTTTACA GCAGCAACGC CAACACTGAA 
GAACAAGAGG AGAACTTTGA GTTTATTATC GTATCCCTTA CAAGTCAGAG CTGGCACTTT 
GAAGCTACAA GCTATGAGGA GAGGGATGCA TGGGTACAGG CCATAGAGAG TCAGATCTTG 
GCCAGCTTAC AGTCCTGCGA AAGCAGCAAG AACAAGTCCC GGCTCACCAG CCAGAATGAA 
GCTTTAGCAC TACAGTCGAT CCGAAATCTG CCAGGAAACT CGCACTGTGT GGACTGTGAT 
GCACAAAACC CAGATTGGGC CAGTCTGAAC CTGGGTGCCC TCATGTGCAT TGAATGTTCT 
GGGATCCACC GTAACCTTGG TACTCACCTT TCCCGTGTCC GTTCCCTGGA TCTGGACGAC 
TGGCCTCCAG AACTAATTAA AGTGATGTCA GCCATCGGAA ATGAGCTGGC CAACAGTGTG 
TGGGAAGGGA GCAGCCAAGG GCATGTAAAG CCATGTTCGG AGAGCCCAAG AGAAGAGAAG 
GAACGCTGGA TACGTGCAAA GTATGAGCAA AAGCTTTTTC TGTCACCGCT ACCCTGCCGG 
GATTTACCTC TGGGACAGCA GCTTCTAAGG GCCACAGCAG AGGAGGATTT GAGAGCTGTA 
ATTTTGCTTC TTGCCCATGG TTCCAGGGAG GAGGTTAATG AGACCTGCGG GGAGGGAGAC 
AGGCGCACAT CCCTACACCT GGCCTGTCGC AAGGGAAATG TAGTACTCGT GCAACTGCTT 
ATATGGTACG GTGTGGATGT GATGGCAAGG GACTTCCATG GAAACACAGC ATTGGCGTAT 
GCCAAACAAG CAGTGACGTC TGAGGTGCGG GAGTTGCTTT TGCAATATGG TTGCCCTGAC 
GAACAATTTG TGCTGATGGC CACGCCAAAC CTGTCTCGGA AAAACAACCG CAACAACAAC 
AGCAATGCAG GGGGCAGTGG CCTGATGCCC ACCTTAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917928.1
CDS	113..2794
Translation	MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERLDEPALQNQIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEAKRKAWKLNRVGSLRNVYSSNANTEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917871.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917871.3

ORF Insert Sequence:

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG TTCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG ATATGGATGC AGGTGGGAGG TTCAAGAAGG AAATTGTAGT AGATGGACAG 
AGCTACCTTC TTCTGATCAG GGATGAAGGG GGACCCCCCG AAGCACAATT TGCCATGTGG 
GTGGATGCAG TTATCTTTGT CTTCAGCCTG GAAGATGAGA TCAGCTTTCA GACAGTCTAT 
CACTACTACA GCCGCCTTGC CAACTACCGC AACACCAGCG AGATCCCTAT GGTGCTGGTA 
GGCACTCAAG ATGCCATTAG CTGCAGTAAC CCCCGTGTAA TTGATGACAG CCGGGCACGA 
AAGCTTTCTA ATGACCTCAA GCGATGCACT TATTATGAGA CCTGCGCCAC CTATGGGCTA 
AATGTGGAGC GTGTTTTCCA AGATGTGGCC CAGAAGATTG TAGCCACCCG TAAGAAACAA 
CAGCTTTCCA TCGGACCCTG CAAGTCCCTG CCAAACTCCC CCAGCCACAC GTCTGTCTGT 
TCTACGCAGG TTTCGGCTGT TCACATTAGT CAGACCAGTA ATGGTGGGGG TAGCCTAAGC 
GACTATTCCT CATCTGTCCC CTCTACACCG AGTACTAGTC AGAAGGAGCT GAGGATCGAC 
GTGCCCCCAG CAGCCAGCAC ACCCACTCCC GTGCGCAAGC AATCAAAACG GCGCTCCAAT 
CTCTTCACGT CTCGCAAAGG GAGCGACCCA GACAAAGATA AGAAAGGTCT AGAGAGCAGA 
GCAGACAGCA TCGGCAGCGG GCGAGCAATA CCAATTAAAC AGGGCATGCT ACTGAAAAGA 
AGCGGAAAGT CTCTTAACAA AGAGTGGAAG AAGAAATACG TAACCTTGTG TGATAACGGA 
GTTCTGACAT ATCATCCCAG CTTACACGAT TACATGCAAA ATGTCCATGG TAAAGAGATC 
GACCTCCTTA GAACTACTGT GAAGGTCCCT GGGAAAAGGC CTCCTCGAGC CACATCCTCT 
TGTGCACCAG TTGCCAGCCC TAAAACCAAT GGTCTAACCA AAGAGGTCAG CGGGCTCCAG 
ATTTCCCCTA ACACAGGGAA CGTGACCAGC AGTACGTCAG TCACCCAAAT GGCCAGTGGT 
CCAAGTGGGA TTAGTTTAGG CTCATTCAGC CGCATGGATG GGATGCACCA GAGGTCATAC 
TCTGTGTCTA GCGCTGACCA GTGGAGCGAG GGAGCCGTCA TCGCCAACTC AGCCATCAGC 
AGCGACACTG GCCTAGGAGA CTCAGTGTGT TCCAGTCCTA GCATCTCCAG CACTACAAGT 
CCCAAGCTAG ATCCGCCACC CTCGCCACAT GCAAACCGCA AGAAGCACCG CCGGAAGAAA 
AGTACCAGTA ACTTTAAGGC TGATGGGCTG TCAAGTACTG CTGAAGCTAA ACGTAAAGCA 
TGGAAACTTA ACCGTGTTGG CAGCCTGCGA AATGTTTACA GCAGCAACGC CAACACTGAA 
GAACAAGAGG AGAACTTTGA GTTTATTATC GTATCCCTTA CAAGTCAGAG CTGGCACTTT 
GAAGCTACAA GCTATGAGGA GAGGGATGCA TGGGTACAGG CCATAGAGAG TCAGATCTTG 
GCCAGCTTAC AGTCCTGCGA AAGCAGCAAG AACAAGTCCC GGCTCACCAG CCAGAATGAA 
GCTTTAGCAC TACAGTCGAT CCGAAATCTG CCAGGAAACT CGCACTGTGT GGACTGTGAT 
GCACAAAACC CAGATTGGGC CAGTCTGAAC CTGGGTGCCC TCATGTGCAT TGAATGTTCT 
GGGATCCACC GTAACCTTGG TACTCACCTT TCCCGTGTCC GTTCCCTGGA TCTGGACGAC 
TGGCCTCCAG AACTAATTAA AGTGATGTCA GCCATCGGAA ATGAGCTGGC CAACAGTGTG 
TGGGAAGGGA GCAGCCAAGG GCATGTAAAG CCATGTTCGG AGAGCCCAAG AGAAGAGAAG 
GAACGCTGGA TACGTGCAAA GTATGAGCAA AAGCTTTTTC TGTCACCGCT ACCCTGCCGG 
GATTTACCTC TGGGACAGCA GCTTCTAAGG GCCACAGCAG AGGAGGATTT GAGAGCTGTA 
ATTTTGCTTC TTGCCCATGG TTCCAGGGAG GAGGTTAATG AGACCTGCGG GGAGGGAGAC 
AGGCGCACAT CCCTACACCT GGCCTGTCGC AAGGGAAATG TAGTACTCGT GCAACTGCTT 
ATATGGTACG GTGTGGATGT GATGGCAAGG GACTTCCATG GAAACACAGC ATTGGCGTAT 
GCCAAACAAG CAGTGACGTC TGAGGTGCGG GAGTTGCTTT TGCAATATGG TTGCCCTGAC 
GAACAATTTG TGCTGATGGC CACGCCAAAC CTGTCTCGGA AAAACAACCG CAACAACAAC 
AGCAATGCAG GGGGCAGTGG CCTGATGCCC ACCTTAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917872.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917929.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa16171
Clone ID Related Accession (Same CDS sequence)	XM_004917872.3
Accession Version	XM_004917872.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917872.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG TTCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA ATTGTAGTAG ATGGACAGAG CTACCTTCTT 
CTGATCAGGG ATGAAGGGGG ACCCCCCGAA GCACAATTTG CCATGTGGGT GGATGCAGTT 
ATCTTTGTCT TCAGCCTGGA AGATGAGATC AGCTTTCAGA CAGTCTATCA CTACTACAGC 
CGCCTTGCCA ACTACCGCAA CACCAGCGAG ATCCCTATGG TGCTGGTAGG CACTCAAGAT 
GCCATTAGCT GCAGTAACCC CCGTGTAATT GATGACAGCC GGGCACGAAA GCTTTCTAAT 
GACCTCAAGC GATGCACTTA TTATGAGACC TGCGCCACCT ATGGGCTAAA TGTGGAGCGT 
GTTTTCCAAG ATGTGGCCCA GAAGATTGTA GCCACCCGTA AGAAACAACA GCTTTCCATC 
GGACCCTGCA AGTCCCTGCC AAACTCCCCC AGCCACACGT CTGTCTGTTC TACGCAGGTT 
TCGGCTGTTC ACATTAGTCA GACCAGTAAT GGTGGGGGTA GCCTAAGCGA CTATTCCTCA 
TCTGTCCCCT CTACACCGAG TACTAGTCAG AAGGAGCTGA GGATCGACGT GCCCCCAGCA 
GCCAGCACAC CCACTCCCGT GCGCAAGCAA TCAAAACGGC GCTCCAATCT CTTCACGTCT 
CGCAAAGGGA GCGACCCAGA CAAAGATAAG AAAGGTCTAG AGAGCAGAGC AGACAGCATC 
GGCAGCGGGC GAGCAATACC AATTAAACAG GGCATGCTAC TGAAAAGAAG CGGAAAGTCT 
CTTAACAAAG AGTGGAAGAA GAAATACGTA ACCTTGTGTG ATAACGGAGT TCTGACATAT 
CATCCCAGCT TACACGATTA CATGCAAAAT GTCCATGGTA AAGAGATCGA CCTCCTTAGA 
ACTACTGTGA AGGTCCCTGG GAAAAGGCCT CCTCGAGCCA CATCCTCTTG TGCACCAGTT 
GCCAGCCCTA AAACCAATGG TCTAACCAAA GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC 
ACAGGGAACG TGACCAGCAG TACGTCAGTC ACCCAAATGG CCAGTGGTCC AAGTGGGATT 
AGTTTAGGCT CATTCAGCCG CATGGATGGG ATGCACCAGA GGTCATACTC TGTGTCTAGC 
GCTGACCAGT GGAGCGAGGG AGCCGTCATC GCCAACTCAG CCATCAGCAG CGACACTGGC 
CTAGGAGACT CAGTGTGTTC CAGTCCTAGC ATCTCCAGCA CTACAAGTCC CAAGCTAGAT 
CCGCCACCCT CGCCACATGC AAACCGCAAG AAGCACCGCC GGAAGAAAAG TACCAGTAAC 
TTTAAGGCTG ATGGGCTGTC AAGTACTGCT GAAGCTAAAC GTAAAGCATG GAAACTTAAC 
CGTGTTGGCA GCCTGCGAAA TGTTTACAGC AGCAACGCCA ACACTGAAGA ACAAGAGGAG 
AACTTTGAGT TTATTATCGT ATCCCTTACA AGTCAGAGCT GGCACTTTGA AGCTACAAGC 
TATGAGGAGA GGGATGCATG GGTACAGGCC ATAGAGAGTC AGATCTTGGC CAGCTTACAG 
TCCTGCGAAA GCAGCAAGAA CAAGTCCCGG CTCACCAGCC AGAATGAAGC TTTAGCACTA 
CAGTCGATCC GAAATCTGCC AGGAAACTCG CACTGTGTGG ACTGTGATGC ACAAAACCCA 
GATTGGGCCA GTCTGAACCT GGGTGCCCTC ATGTGCATTG AATGTTCTGG GATCCACCGT 
AACCTTGGTA CTCACCTTTC CCGTGTCCGT TCCCTGGATC TGGACGACTG GCCTCCAGAA 
CTAATTAAAG TGATGTCAGC CATCGGAAAT GAGCTGGCCA ACAGTGTGTG GGAAGGGAGC 
AGCCAAGGGC ATGTAAAGCC ATGTTCGGAG AGCCCAAGAG AAGAGAAGGA ACGCTGGATA 
CGTGCAAAGT ATGAGCAAAA GCTTTTTCTG TCACCGCTAC CCTGCCGGGA TTTACCTCTG 
GGACAGCAGC TTCTAAGGGC CACAGCAGAG GAGGATTTGA GAGCTGTAAT TTTGCTTCTT 
GCCCATGGTT CCAGGGAGGA GGTTAATGAG ACCTGCGGGG AGGGAGACAG GCGCACATCC 
CTACACCTGG CCTGTCGCAA GGGAAATGTA GTACTCGTGC AACTGCTTAT ATGGTACGGT 
GTGGATGTGA TGGCAAGGGA CTTCCATGGA AACACAGCAT TGGCGTATGC CAAACAAGCA 
GTGACGTCTG AGGTGCGGGA GTTGCTTTTG CAATATGGTT GCCCTGACGA ACAATTTGTG 
CTGATGGCCA CGCCAAACCT GTCTCGGAAA AACAACCGCA ACAACAACAG CAATGCAGGG 
GGCAGTGGCC TGATGCCCAC CTTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917929.1
CDS	113..2782
Translation	MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERLDEPALQNQIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEAKRKAWKLNRVGSLRNVYSSNANTEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917872.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917872.3

ORF Insert Sequence:

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG TTCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA ATTGTAGTAG ATGGACAGAG CTACCTTCTT 
CTGATCAGGG ATGAAGGGGG ACCCCCCGAA GCACAATTTG CCATGTGGGT GGATGCAGTT 
ATCTTTGTCT TCAGCCTGGA AGATGAGATC AGCTTTCAGA CAGTCTATCA CTACTACAGC 
CGCCTTGCCA ACTACCGCAA CACCAGCGAG ATCCCTATGG TGCTGGTAGG CACTCAAGAT 
GCCATTAGCT GCAGTAACCC CCGTGTAATT GATGACAGCC GGGCACGAAA GCTTTCTAAT 
GACCTCAAGC GATGCACTTA TTATGAGACC TGCGCCACCT ATGGGCTAAA TGTGGAGCGT 
GTTTTCCAAG ATGTGGCCCA GAAGATTGTA GCCACCCGTA AGAAACAACA GCTTTCCATC 
GGACCCTGCA AGTCCCTGCC AAACTCCCCC AGCCACACGT CTGTCTGTTC TACGCAGGTT 
TCGGCTGTTC ACATTAGTCA GACCAGTAAT GGTGGGGGTA GCCTAAGCGA CTATTCCTCA 
TCTGTCCCCT CTACACCGAG TACTAGTCAG AAGGAGCTGA GGATCGACGT GCCCCCAGCA 
GCCAGCACAC CCACTCCCGT GCGCAAGCAA TCAAAACGGC GCTCCAATCT CTTCACGTCT 
CGCAAAGGGA GCGACCCAGA CAAAGATAAG AAAGGTCTAG AGAGCAGAGC AGACAGCATC 
GGCAGCGGGC GAGCAATACC AATTAAACAG GGCATGCTAC TGAAAAGAAG CGGAAAGTCT 
CTTAACAAAG AGTGGAAGAA GAAATACGTA ACCTTGTGTG ATAACGGAGT TCTGACATAT 
CATCCCAGCT TACACGATTA CATGCAAAAT GTCCATGGTA AAGAGATCGA CCTCCTTAGA 
ACTACTGTGA AGGTCCCTGG GAAAAGGCCT CCTCGAGCCA CATCCTCTTG TGCACCAGTT 
GCCAGCCCTA AAACCAATGG TCTAACCAAA GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC 
ACAGGGAACG TGACCAGCAG TACGTCAGTC ACCCAAATGG CCAGTGGTCC AAGTGGGATT 
AGTTTAGGCT CATTCAGCCG CATGGATGGG ATGCACCAGA GGTCATACTC TGTGTCTAGC 
GCTGACCAGT GGAGCGAGGG AGCCGTCATC GCCAACTCAG CCATCAGCAG CGACACTGGC 
CTAGGAGACT CAGTGTGTTC CAGTCCTAGC ATCTCCAGCA CTACAAGTCC CAAGCTAGAT 
CCGCCACCCT CGCCACATGC AAACCGCAAG AAGCACCGCC GGAAGAAAAG TACCAGTAAC 
TTTAAGGCTG ATGGGCTGTC AAGTACTGCT GAAGCTAAAC GTAAAGCATG GAAACTTAAC 
CGTGTTGGCA GCCTGCGAAA TGTTTACAGC AGCAACGCCA ACACTGAAGA ACAAGAGGAG 
AACTTTGAGT TTATTATCGT ATCCCTTACA AGTCAGAGCT GGCACTTTGA AGCTACAAGC 
TATGAGGAGA GGGATGCATG GGTACAGGCC ATAGAGAGTC AGATCTTGGC CAGCTTACAG 
TCCTGCGAAA GCAGCAAGAA CAAGTCCCGG CTCACCAGCC AGAATGAAGC TTTAGCACTA 
CAGTCGATCC GAAATCTGCC AGGAAACTCG CACTGTGTGG ACTGTGATGC ACAAAACCCA 
GATTGGGCCA GTCTGAACCT GGGTGCCCTC ATGTGCATTG AATGTTCTGG GATCCACCGT 
AACCTTGGTA CTCACCTTTC CCGTGTCCGT TCCCTGGATC TGGACGACTG GCCTCCAGAA 
CTAATTAAAG TGATGTCAGC CATCGGAAAT GAGCTGGCCA ACAGTGTGTG GGAAGGGAGC 
AGCCAAGGGC ATGTAAAGCC ATGTTCGGAG AGCCCAAGAG AAGAGAAGGA ACGCTGGATA 
CGTGCAAAGT ATGAGCAAAA GCTTTTTCTG TCACCGCTAC CCTGCCGGGA TTTACCTCTG 
GGACAGCAGC TTCTAAGGGC CACAGCAGAG GAGGATTTGA GAGCTGTAAT TTTGCTTCTT 
GCCCATGGTT CCAGGGAGGA GGTTAATGAG ACCTGCGGGG AGGGAGACAG GCGCACATCC 
CTACACCTGG CCTGTCGCAA GGGAAATGTA GTACTCGTGC AACTGCTTAT ATGGTACGGT 
GTGGATGTGA TGGCAAGGGA CTTCCATGGA AACACAGCAT TGGCGTATGC CAAACAAGCA 
GTGACGTCTG AGGTGCGGGA GTTGCTTTTG CAATATGGTT GCCCTGACGA ACAATTTGTG 
CTGATGGCCA CGCCAAACCT GTCTCGGAAA AACAACCGCA ACAACAACAG CAATGCAGGG 
GGCAGTGGCC TGATGCCCAC CTTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_002931998.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932044.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X6, mRNA.

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CloneID	OXa16172
Clone ID Related Accession (Same CDS sequence)	XM_002931998.4
Accession Version	XM_002931998.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931998.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG TTCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA ATTGTAGTAG ATGGACAGAG CTACCTTCTT 
CTGATCAGGG ATGAAGGGGG ACCCCCCGAA GCACAATTTG CCATGTGGGT GGATGCAGTT 
ATCTTTGTCT TCAGCCTGGA AGATGAGATC AGCTTTCAGA CAGTCTATCA CTACTACAGC 
CGCCTTGCCA ACTACCGCAA CACCAGCGAG ATCCCTATGG TGCTGGTAGG CACTCAAGAT 
GCCATTAGCT GCAGTAACCC CCGTGTAATT GATGACAGCC GGGCACGAAA GCTTTCTAAT 
GACCTCAAGC GATGCACTTA TTATGAGACC TGCGCCACCT ATGGGCTAAA TGTGGAGCGT 
GTTTTCCAAG ATGTGGCCCA GAAGATTGTA GCCACCCGTA AGAAACAACA GCTTTCCATC 
GGACCCTGCA AGTCCCTGCC AAACTCCCCC AGCCACACGT CTGTCTGTTC TACGCAGGTT 
TCGGCTGTTC ACATTAGTCA GACCAGTAAT GGTGGGGGTA GCCTAAGCGA CTATTCCTCA 
TCTGTCCCCT CTACACCGAG TACTAGTCAG AAGGAGCTGA GGATCGACGT GCCCCCAGCA 
GCCAGCACAC CCACTCCCGT GCGCAAGCAA TCAAAACGGC GCTCCAATCT CTTCACGTCT 
CGCAAAGGGA GCGACCCAGA CAAAGATAAG AAAGGTCTAG AGAGCAGAGC AGACAGCATC 
GGCAGCGGGC GAGCAATACC AATTAAACAG GGCATGCTAC TGAAAAGAAG CGGAAAGTCT 
CTTAACAAAG AGTGGAAGAA GAAATACGTA ACCTTGTGTG ATAACGGAGT TCTGACATAT 
CATCCCAGCT TACACGATTA CATGCAAAAT GTCCATGGTA AAGAGATCGA CCTCCTTAGA 
ACTACTGTGA AGGTCCCTGG GAAAAGGCCT CCTCGAGCCA CATCCTCTTG TGCACCAGTT 
GCCAGCCCTA AAACCAATGG TCTAACCAAA GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC 
ACAGGGAACG TGACCAGCAG TACGTCAGTC ACCCAAATGG CCAGTGGTCC AAGTGGGATT 
AGTTTAGGCT CATTCAGCCG CATGGATGGG ATGCACCAGA GGTCATACTC TGTGTCTAGC 
GCTGACCAGT GGAGCGAGGG AGCCGTCATC GCCAACTCAG CCATCAGCAG CGACACTGGC 
CTAGGAGACT CAGTGTGTTC CAGTCCTAGC ATCTCCAGCA CTACAAGTCC CAAGCTAGAT 
CCGCCACCCT CGCCACATGC AAACCGCAAG AAGCACCGCC GGAAGAAAAG TACCAGTAAC 
TTTAAGGCTG ATGGGCTGTC AAGTACTGCT GAAGAACAAG AGGAGAACTT TGAGTTTATT 
ATCGTATCCC TTACAAGTCA GAGCTGGCAC TTTGAAGCTA CAAGCTATGA GGAGAGGGAT 
GCATGGGTAC AGGCCATAGA GAGTCAGATC TTGGCCAGCT TACAGTCCTG CGAAAGCAGC 
AAGAACAAGT CCCGGCTCAC CAGCCAGAAT GAAGCTTTAG CACTACAGTC GATCCGAAAT 
CTGCCAGGAA ACTCGCACTG TGTGGACTGT GATGCACAAA ACCCAGATTG GGCCAGTCTG 
AACCTGGGTG CCCTCATGTG CATTGAATGT TCTGGGATCC ACCGTAACCT TGGTACTCAC 
CTTTCCCGTG TCCGTTCCCT GGATCTGGAC GACTGGCCTC CAGAACTAAT TAAAGTGATG 
TCAGCCATCG GAAATGAGCT GGCCAACAGT GTGTGGGAAG GGAGCAGCCA AGGGCATGTA 
AAGCCATGTT CGGAGAGCCC AAGAGAAGAG AAGGAACGCT GGATACGTGC AAAGTATGAG 
CAAAAGCTTT TTCTGTCACC GCTACCCTGC CGGGATTTAC CTCTGGGACA GCAGCTTCTA 
AGGGCCACAG CAGAGGAGGA TTTGAGAGCT GTAATTTTGC TTCTTGCCCA TGGTTCCAGG 
GAGGAGGTTA ATGAGACCTG CGGGGAGGGA GACAGGCGCA CATCCCTACA CCTGGCCTGT 
CGCAAGGGAA ATGTAGTACT CGTGCAACTG CTTATATGGT ACGGTGTGGA TGTGATGGCA 
AGGGACTTCC ATGGAAACAC AGCATTGGCG TATGCCAAAC AAGCAGTGAC GTCTGAGGTG 
CGGGAGTTGC TTTTGCAATA TGGTTGCCCT GACGAACAAT TTGTGCTGAT GGCCACGCCA 
AACCTGTCTC GGAAAAACAA CCGCAACAAC AACAGCAATG CAGGGGGCAG TGGCCTGATG 
CCCACCTTAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932044.1
CDS	1..2595
Translation	MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERLDEPALQNQIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_002931998.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931998.4

ORF Insert Sequence:

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG TTCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA ATTGTAGTAG ATGGACAGAG CTACCTTCTT 
CTGATCAGGG ATGAAGGGGG ACCCCCCGAA GCACAATTTG CCATGTGGGT GGATGCAGTT 
ATCTTTGTCT TCAGCCTGGA AGATGAGATC AGCTTTCAGA CAGTCTATCA CTACTACAGC 
CGCCTTGCCA ACTACCGCAA CACCAGCGAG ATCCCTATGG TGCTGGTAGG CACTCAAGAT 
GCCATTAGCT GCAGTAACCC CCGTGTAATT GATGACAGCC GGGCACGAAA GCTTTCTAAT 
GACCTCAAGC GATGCACTTA TTATGAGACC TGCGCCACCT ATGGGCTAAA TGTGGAGCGT 
GTTTTCCAAG ATGTGGCCCA GAAGATTGTA GCCACCCGTA AGAAACAACA GCTTTCCATC 
GGACCCTGCA AGTCCCTGCC AAACTCCCCC AGCCACACGT CTGTCTGTTC TACGCAGGTT 
TCGGCTGTTC ACATTAGTCA GACCAGTAAT GGTGGGGGTA GCCTAAGCGA CTATTCCTCA 
TCTGTCCCCT CTACACCGAG TACTAGTCAG AAGGAGCTGA GGATCGACGT GCCCCCAGCA 
GCCAGCACAC CCACTCCCGT GCGCAAGCAA TCAAAACGGC GCTCCAATCT CTTCACGTCT 
CGCAAAGGGA GCGACCCAGA CAAAGATAAG AAAGGTCTAG AGAGCAGAGC AGACAGCATC 
GGCAGCGGGC GAGCAATACC AATTAAACAG GGCATGCTAC TGAAAAGAAG CGGAAAGTCT 
CTTAACAAAG AGTGGAAGAA GAAATACGTA ACCTTGTGTG ATAACGGAGT TCTGACATAT 
CATCCCAGCT TACACGATTA CATGCAAAAT GTCCATGGTA AAGAGATCGA CCTCCTTAGA 
ACTACTGTGA AGGTCCCTGG GAAAAGGCCT CCTCGAGCCA CATCCTCTTG TGCACCAGTT 
GCCAGCCCTA AAACCAATGG TCTAACCAAA GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC 
ACAGGGAACG TGACCAGCAG TACGTCAGTC ACCCAAATGG CCAGTGGTCC AAGTGGGATT 
AGTTTAGGCT CATTCAGCCG CATGGATGGG ATGCACCAGA GGTCATACTC TGTGTCTAGC 
GCTGACCAGT GGAGCGAGGG AGCCGTCATC GCCAACTCAG CCATCAGCAG CGACACTGGC 
CTAGGAGACT CAGTGTGTTC CAGTCCTAGC ATCTCCAGCA CTACAAGTCC CAAGCTAGAT 
CCGCCACCCT CGCCACATGC AAACCGCAAG AAGCACCGCC GGAAGAAAAG TACCAGTAAC 
TTTAAGGCTG ATGGGCTGTC AAGTACTGCT GAAGAACAAG AGGAGAACTT TGAGTTTATT 
ATCGTATCCC TTACAAGTCA GAGCTGGCAC TTTGAAGCTA CAAGCTATGA GGAGAGGGAT 
GCATGGGTAC AGGCCATAGA GAGTCAGATC TTGGCCAGCT TACAGTCCTG CGAAAGCAGC 
AAGAACAAGT CCCGGCTCAC CAGCCAGAAT GAAGCTTTAG CACTACAGTC GATCCGAAAT 
CTGCCAGGAA ACTCGCACTG TGTGGACTGT GATGCACAAA ACCCAGATTG GGCCAGTCTG 
AACCTGGGTG CCCTCATGTG CATTGAATGT TCTGGGATCC ACCGTAACCT TGGTACTCAC 
CTTTCCCGTG TCCGTTCCCT GGATCTGGAC GACTGGCCTC CAGAACTAAT TAAAGTGATG 
TCAGCCATCG GAAATGAGCT GGCCAACAGT GTGTGGGAAG GGAGCAGCCA AGGGCATGTA 
AAGCCATGTT CGGAGAGCCC AAGAGAAGAG AAGGAACGCT GGATACGTGC AAAGTATGAG 
CAAAAGCTTT TTCTGTCACC GCTACCCTGC CGGGATTTAC CTCTGGGACA GCAGCTTCTA 
AGGGCCACAG CAGAGGAGGA TTTGAGAGCT GTAATTTTGC TTCTTGCCCA TGGTTCCAGG 
GAGGAGGTTA ATGAGACCTG CGGGGAGGGA GACAGGCGCA CATCCCTACA CCTGGCCTGT 
CGCAAGGGAA ATGTAGTACT CGTGCAACTG CTTATATGGT ACGGTGTGGA TGTGATGGCA 
AGGGACTTCC ATGGAAACAC AGCATTGGCG TATGCCAAAC AAGCAGTGAC GTCTGAGGTG 
CGGGAGTTGC TTTTGCAATA TGGTTGCCCT GACGAACAAT TTGTGCTGAT GGCCACGCCA 
AACCTGTCTC GGAAAAACAA CCGCAACAAC AACAGCAATG CAGGGGGCAG TGGCCTGATG 
CCCACCTTAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917871.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917928.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa66205
Clone ID Related Accession (Same CDS sequence)	XM_004917871.4
Accession Version	XM_004917871.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917871.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG ATCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG ATATGGATGC AGGTGGGAGG TTCAAGAAGG AAATTGTAGT AGATGGACAG 
AGCTACCTTC TTCTGATCAG GGATGAAGGG GGACCCCCCG AAGCACAATT TGCCATGTGG 
GTGGATGCAG TTATCTTTGT CTTCAGCCTG GAAGATGAGA TCAGCTTTCA GACAGTCTAT 
CACTACTACA GCCGCCTTGC CAACTACCGC AACACCAGCG AGATCCCTAT GGTGCTGGTA 
GGCACTCAAG ATGCCATTAG CTGCAGTAAC CCCCGTGTAA TTGATGACAG CCGGGCACGA 
AAGCTTTCTA ATGACCTCAA GCGATGCACT TATTATGAGA CCTGCGCCAC CTATGGGCTA 
AATGTGGAGC GTGTTTTCCA AGATGTGGCC CAGAAGATTG TAGCCACCCG TAAGAAACAA 
CAGCTTTCCA TCGGACCCTG CAAGTCCCTG CCAAACTCCC CCAGCCACAC GTCTGTCTGT 
TCTACGCAGG TTTCGGCTGT TCACATTAGT CAGACCAGTA ATGGTGGGGG TAGCCTAAGC 
GACTATTCCT CATCTGTCCC CTCTACACCG AGTACTAGTC AGAAGGAGCT GAGGATCGAC 
GTGCCCCCAG CAGCCAGCAC ACCCACTCCC GTGCGCAAGC AATCAAAACG GCGCTCCAAT 
CTCTTCACGT CTCGCAAAGG GAGCGACCCA GACAAAGATA AGAAAGGTCT AGAGAGCAGA 
GCAGACAGCA TCGGCAGCGG GCGAGCAATA CCAATTAAAC AGGGCATGCT ACTGAAAAGA 
AGCGGAAAGT CTCTTAACAA AGAGTGGAAG AAGAAATACG TAACCTTGTG TGATAACGGA 
GTTCTGACAT ATCATCCCAG CTTACACGAT TACATGCAAA ATGTCCATGG TAAAGAGATC 
GACCTCCTTA GAACTACTGT GAAGGTCCCT GGGAAAAGGC CTCCTCGAGC CACATCCTCT 
TGTGCACCAG TTGCCAGCCC TAAAACCAAT GGTCTAACCA AAGAGGTCAG CGGGCTCCAG 
ATTTCCCCTA ACACAGGGAA CGTGACCAGC AGTACGTCAG TCACCCAAAT GGCCAGTGGT 
CCAAGTGGGA TTAGTTTAGG CTCATTCAGC CGCATGGATG GGATGCACCA GAGGTCATAC 
TCTGTGTCTA GCGCTGACCA GTGGAGCGAG GGAGCCGTCA TCGCCAACTC AGCCATCAGC 
AGCGACACTG GCCTAGGAGA CTCAGTGTGT TCCAGTCCTA GCATCTCCAG CACTACAAGT 
CCCAAGCTAG ATCCGCCACC CTCGCCACAC GCAAACCGCA AGAAGCACCG CCGGAAGAAA 
AGTACCAGTA ACTTTAAGGC TGATGGGCTG TCAAGTACTG CTGAAGCTAA ACGTAAAGCA 
TGGAAACTTA ACCGTGTTGG CAGCCTGCGA AATGTTTACA GCAGCAACGC CAACACTGAA 
GAACAAGAGG AGAACTTTGA GTTTATTATC GTATCCCTTA CAAGTCAGAG CTGGCACTTT 
GAAGCTACAA GCTATGAGGA GAGGGATGCA TGGGTACAGG CCATAGAGAG TCAGATCTTG 
GCCAGCTTAC AGTCCTGCGA AAGCAGCAAG AACAAGTCCC GGCTCACCAG CCAGAATGAA 
GCTTTAGCAC TACAGTCGAT CCGAAATCTG CCAGGAAACT CGCACTGTGT GGACTGTGAT 
GCACAAAACC CAGATTGGGC CAGTCTGAAC CTGGGTGCCC TCATGTGCAT TGAATGTTCT 
GGGATCCACC GTAACCTTGG TACTCACCTT TCCCGTGTCC GTTCCCTGGA TCTGGACGAC 
TGGCCTCCAG AACTAATTAA AGTGATGTCA GCCATCGGAA ATGAGCTGGC CAACAGTGTG 
TGGGAAGGGA GCAGCCAAGG GCATGTAAAG CCATGTTCGG AGAGCCCAAG AGAAGAGAAG 
GAACGCTGGA TACGTGCAAA GTATGAGCAA AAGCTTTTTC TGTCACCGCT ACCCTGCCGG 
GATTTACCTC TGGGACAGCA GCTTCTAAGG GCCACAGCAG AGGAGGATTT GAGAGCTGTA 
ATTTTGCTTC TTGCCCATGG TTCCAGGGAG GAGGTTAATG AGACCTGCGG GGAGGGAGAC 
AGGCGCACAT CCCTACACCT GGCCTGTCGC AAGGGAAATG TAGTACTCGT GCAACTGCTT 
ATATGGTACG GTGTGGATGT GATGGCAAGG GACTTCCATG GAAACACAGC ATTGGCGTAT 
GCCAAACAAG CAGTGACGTC TGAGGTGCGG GAGTTGCTTT TGCAATATGG TTGCCCTGAC 
GAACAATTTG TGCTGATGGC CACGCCAAAC CTGTCTCGGA AAAACAACCG CAACAACAAC 
AGCAATGCAG GGGGCAGTGG CCTGATGCCC ACCTTAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917928.1
CDS	1231..3912
Translation	MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERLDEPALQNQIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEAKRKAWKLNRVGSLRNVYSSNANTEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917871.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917871.4

ORF Insert Sequence:

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG ATCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG ATATGGATGC AGGTGGGAGG TTCAAGAAGG AAATTGTAGT AGATGGACAG 
AGCTACCTTC TTCTGATCAG GGATGAAGGG GGACCCCCCG AAGCACAATT TGCCATGTGG 
GTGGATGCAG TTATCTTTGT CTTCAGCCTG GAAGATGAGA TCAGCTTTCA GACAGTCTAT 
CACTACTACA GCCGCCTTGC CAACTACCGC AACACCAGCG AGATCCCTAT GGTGCTGGTA 
GGCACTCAAG ATGCCATTAG CTGCAGTAAC CCCCGTGTAA TTGATGACAG CCGGGCACGA 
AAGCTTTCTA ATGACCTCAA GCGATGCACT TATTATGAGA CCTGCGCCAC CTATGGGCTA 
AATGTGGAGC GTGTTTTCCA AGATGTGGCC CAGAAGATTG TAGCCACCCG TAAGAAACAA 
CAGCTTTCCA TCGGACCCTG CAAGTCCCTG CCAAACTCCC CCAGCCACAC GTCTGTCTGT 
TCTACGCAGG TTTCGGCTGT TCACATTAGT CAGACCAGTA ATGGTGGGGG TAGCCTAAGC 
GACTATTCCT CATCTGTCCC CTCTACACCG AGTACTAGTC AGAAGGAGCT GAGGATCGAC 
GTGCCCCCAG CAGCCAGCAC ACCCACTCCC GTGCGCAAGC AATCAAAACG GCGCTCCAAT 
CTCTTCACGT CTCGCAAAGG GAGCGACCCA GACAAAGATA AGAAAGGTCT AGAGAGCAGA 
GCAGACAGCA TCGGCAGCGG GCGAGCAATA CCAATTAAAC AGGGCATGCT ACTGAAAAGA 
AGCGGAAAGT CTCTTAACAA AGAGTGGAAG AAGAAATACG TAACCTTGTG TGATAACGGA 
GTTCTGACAT ATCATCCCAG CTTACACGAT TACATGCAAA ATGTCCATGG TAAAGAGATC 
GACCTCCTTA GAACTACTGT GAAGGTCCCT GGGAAAAGGC CTCCTCGAGC CACATCCTCT 
TGTGCACCAG TTGCCAGCCC TAAAACCAAT GGTCTAACCA AAGAGGTCAG CGGGCTCCAG 
ATTTCCCCTA ACACAGGGAA CGTGACCAGC AGTACGTCAG TCACCCAAAT GGCCAGTGGT 
CCAAGTGGGA TTAGTTTAGG CTCATTCAGC CGCATGGATG GGATGCACCA GAGGTCATAC 
TCTGTGTCTA GCGCTGACCA GTGGAGCGAG GGAGCCGTCA TCGCCAACTC AGCCATCAGC 
AGCGACACTG GCCTAGGAGA CTCAGTGTGT TCCAGTCCTA GCATCTCCAG CACTACAAGT 
CCCAAGCTAG ATCCGCCACC CTCGCCACAC GCAAACCGCA AGAAGCACCG CCGGAAGAAA 
AGTACCAGTA ACTTTAAGGC TGATGGGCTG TCAAGTACTG CTGAAGCTAA ACGTAAAGCA 
TGGAAACTTA ACCGTGTTGG CAGCCTGCGA AATGTTTACA GCAGCAACGC CAACACTGAA 
GAACAAGAGG AGAACTTTGA GTTTATTATC GTATCCCTTA CAAGTCAGAG CTGGCACTTT 
GAAGCTACAA GCTATGAGGA GAGGGATGCA TGGGTACAGG CCATAGAGAG TCAGATCTTG 
GCCAGCTTAC AGTCCTGCGA AAGCAGCAAG AACAAGTCCC GGCTCACCAG CCAGAATGAA 
GCTTTAGCAC TACAGTCGAT CCGAAATCTG CCAGGAAACT CGCACTGTGT GGACTGTGAT 
GCACAAAACC CAGATTGGGC CAGTCTGAAC CTGGGTGCCC TCATGTGCAT TGAATGTTCT 
GGGATCCACC GTAACCTTGG TACTCACCTT TCCCGTGTCC GTTCCCTGGA TCTGGACGAC 
TGGCCTCCAG AACTAATTAA AGTGATGTCA GCCATCGGAA ATGAGCTGGC CAACAGTGTG 
TGGGAAGGGA GCAGCCAAGG GCATGTAAAG CCATGTTCGG AGAGCCCAAG AGAAGAGAAG 
GAACGCTGGA TACGTGCAAA GTATGAGCAA AAGCTTTTTC TGTCACCGCT ACCCTGCCGG 
GATTTACCTC TGGGACAGCA GCTTCTAAGG GCCACAGCAG AGGAGGATTT GAGAGCTGTA 
ATTTTGCTTC TTGCCCATGG TTCCAGGGAG GAGGTTAATG AGACCTGCGG GGAGGGAGAC 
AGGCGCACAT CCCTACACCT GGCCTGTCGC AAGGGAAATG TAGTACTCGT GCAACTGCTT 
ATATGGTACG GTGTGGATGT GATGGCAAGG GACTTCCATG GAAACACAGC ATTGGCGTAT 
GCCAAACAAG CAGTGACGTC TGAGGTGCGG GAGTTGCTTT TGCAATATGG TTGCCCTGAC 
GAACAATTTG TGCTGATGGC CACGCCAAAC CTGTCTCGGA AAAACAACCG CAACAACAAC 
AGCAATGCAG GGGGCAGTGG CCTGATGCCC ACCTTAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917872.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917929.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa66206
Clone ID Related Accession (Same CDS sequence)	XM_004917872.4
Accession Version	XM_004917872.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917872.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG ATCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA ATTGTAGTAG ATGGACAGAG CTACCTTCTT 
CTGATCAGGG ATGAAGGGGG ACCCCCCGAA GCACAATTTG CCATGTGGGT GGATGCAGTT 
ATCTTTGTCT TCAGCCTGGA AGATGAGATC AGCTTTCAGA CAGTCTATCA CTACTACAGC 
CGCCTTGCCA ACTACCGCAA CACCAGCGAG ATCCCTATGG TGCTGGTAGG CACTCAAGAT 
GCCATTAGCT GCAGTAACCC CCGTGTAATT GATGACAGCC GGGCACGAAA GCTTTCTAAT 
GACCTCAAGC GATGCACTTA TTATGAGACC TGCGCCACCT ATGGGCTAAA TGTGGAGCGT 
GTTTTCCAAG ATGTGGCCCA GAAGATTGTA GCCACCCGTA AGAAACAACA GCTTTCCATC 
GGACCCTGCA AGTCCCTGCC AAACTCCCCC AGCCACACGT CTGTCTGTTC TACGCAGGTT 
TCGGCTGTTC ACATTAGTCA GACCAGTAAT GGTGGGGGTA GCCTAAGCGA CTATTCCTCA 
TCTGTCCCCT CTACACCGAG TACTAGTCAG AAGGAGCTGA GGATCGACGT GCCCCCAGCA 
GCCAGCACAC CCACTCCCGT GCGCAAGCAA TCAAAACGGC GCTCCAATCT CTTCACGTCT 
CGCAAAGGGA GCGACCCAGA CAAAGATAAG AAAGGTCTAG AGAGCAGAGC AGACAGCATC 
GGCAGCGGGC GAGCAATACC AATTAAACAG GGCATGCTAC TGAAAAGAAG CGGAAAGTCT 
CTTAACAAAG AGTGGAAGAA GAAATACGTA ACCTTGTGTG ATAACGGAGT TCTGACATAT 
CATCCCAGCT TACACGATTA CATGCAAAAT GTCCATGGTA AAGAGATCGA CCTCCTTAGA 
ACTACTGTGA AGGTCCCTGG GAAAAGGCCT CCTCGAGCCA CATCCTCTTG TGCACCAGTT 
GCCAGCCCTA AAACCAATGG TCTAACCAAA GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC 
ACAGGGAACG TGACCAGCAG TACGTCAGTC ACCCAAATGG CCAGTGGTCC AAGTGGGATT 
AGTTTAGGCT CATTCAGCCG CATGGATGGG ATGCACCAGA GGTCATACTC TGTGTCTAGC 
GCTGACCAGT GGAGCGAGGG AGCCGTCATC GCCAACTCAG CCATCAGCAG CGACACTGGC 
CTAGGAGACT CAGTGTGTTC CAGTCCTAGC ATCTCCAGCA CTACAAGTCC CAAGCTAGAT 
CCGCCACCCT CGCCACACGC AAACCGCAAG AAGCACCGCC GGAAGAAAAG TACCAGTAAC 
TTTAAGGCTG ATGGGCTGTC AAGTACTGCT GAAGCTAAAC GTAAAGCATG GAAACTTAAC 
CGTGTTGGCA GCCTGCGAAA TGTTTACAGC AGCAACGCCA ACACTGAAGA ACAAGAGGAG 
AACTTTGAGT TTATTATCGT ATCCCTTACA AGTCAGAGCT GGCACTTTGA AGCTACAAGC 
TATGAGGAGA GGGATGCATG GGTACAGGCC ATAGAGAGTC AGATCTTGGC CAGCTTACAG 
TCCTGCGAAA GCAGCAAGAA CAAGTCCCGG CTCACCAGCC AGAATGAAGC TTTAGCACTA 
CAGTCGATCC GAAATCTGCC AGGAAACTCG CACTGTGTGG ACTGTGATGC ACAAAACCCA 
GATTGGGCCA GTCTGAACCT GGGTGCCCTC ATGTGCATTG AATGTTCTGG GATCCACCGT 
AACCTTGGTA CTCACCTTTC CCGTGTCCGT TCCCTGGATC TGGACGACTG GCCTCCAGAA 
CTAATTAAAG TGATGTCAGC CATCGGAAAT GAGCTGGCCA ACAGTGTGTG GGAAGGGAGC 
AGCCAAGGGC ATGTAAAGCC ATGTTCGGAG AGCCCAAGAG AAGAGAAGGA ACGCTGGATA 
CGTGCAAAGT ATGAGCAAAA GCTTTTTCTG TCACCGCTAC CCTGCCGGGA TTTACCTCTG 
GGACAGCAGC TTCTAAGGGC CACAGCAGAG GAGGATTTGA GAGCTGTAAT TTTGCTTCTT 
GCCCATGGTT CCAGGGAGGA GGTTAATGAG ACCTGCGGGG AGGGAGACAG GCGCACATCC 
CTACACCTGG CCTGTCGCAA GGGAAATGTA GTACTCGTGC AACTGCTTAT ATGGTACGGT 
GTGGATGTGA TGGCAAGGGA CTTCCATGGA AACACAGCAT TGGCGTATGC CAAACAAGCA 
GTGACGTCTG AGGTGCGGGA GTTGCTTTTG CAATATGGTT GCCCTGACGA ACAATTTGTG 
CTGATGGCCA CGCCAAACCT GTCTCGGAAA AACAACCGCA ACAACAACAG CAATGCAGGG 
GGCAGTGGCC TGATGCCCAC CTTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917929.1
CDS	1230..3899
Translation	MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERLDEPALQNQIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEAKRKAWKLNRVGSLRNVYSSNANTEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917872.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917872.4

ORF Insert Sequence:

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG ATCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA ATTGTAGTAG ATGGACAGAG CTACCTTCTT 
CTGATCAGGG ATGAAGGGGG ACCCCCCGAA GCACAATTTG CCATGTGGGT GGATGCAGTT 
ATCTTTGTCT TCAGCCTGGA AGATGAGATC AGCTTTCAGA CAGTCTATCA CTACTACAGC 
CGCCTTGCCA ACTACCGCAA CACCAGCGAG ATCCCTATGG TGCTGGTAGG CACTCAAGAT 
GCCATTAGCT GCAGTAACCC CCGTGTAATT GATGACAGCC GGGCACGAAA GCTTTCTAAT 
GACCTCAAGC GATGCACTTA TTATGAGACC TGCGCCACCT ATGGGCTAAA TGTGGAGCGT 
GTTTTCCAAG ATGTGGCCCA GAAGATTGTA GCCACCCGTA AGAAACAACA GCTTTCCATC 
GGACCCTGCA AGTCCCTGCC AAACTCCCCC AGCCACACGT CTGTCTGTTC TACGCAGGTT 
TCGGCTGTTC ACATTAGTCA GACCAGTAAT GGTGGGGGTA GCCTAAGCGA CTATTCCTCA 
TCTGTCCCCT CTACACCGAG TACTAGTCAG AAGGAGCTGA GGATCGACGT GCCCCCAGCA 
GCCAGCACAC CCACTCCCGT GCGCAAGCAA TCAAAACGGC GCTCCAATCT CTTCACGTCT 
CGCAAAGGGA GCGACCCAGA CAAAGATAAG AAAGGTCTAG AGAGCAGAGC AGACAGCATC 
GGCAGCGGGC GAGCAATACC AATTAAACAG GGCATGCTAC TGAAAAGAAG CGGAAAGTCT 
CTTAACAAAG AGTGGAAGAA GAAATACGTA ACCTTGTGTG ATAACGGAGT TCTGACATAT 
CATCCCAGCT TACACGATTA CATGCAAAAT GTCCATGGTA AAGAGATCGA CCTCCTTAGA 
ACTACTGTGA AGGTCCCTGG GAAAAGGCCT CCTCGAGCCA CATCCTCTTG TGCACCAGTT 
GCCAGCCCTA AAACCAATGG TCTAACCAAA GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC 
ACAGGGAACG TGACCAGCAG TACGTCAGTC ACCCAAATGG CCAGTGGTCC AAGTGGGATT 
AGTTTAGGCT CATTCAGCCG CATGGATGGG ATGCACCAGA GGTCATACTC TGTGTCTAGC 
GCTGACCAGT GGAGCGAGGG AGCCGTCATC GCCAACTCAG CCATCAGCAG CGACACTGGC 
CTAGGAGACT CAGTGTGTTC CAGTCCTAGC ATCTCCAGCA CTACAAGTCC CAAGCTAGAT 
CCGCCACCCT CGCCACACGC AAACCGCAAG AAGCACCGCC GGAAGAAAAG TACCAGTAAC 
TTTAAGGCTG ATGGGCTGTC AAGTACTGCT GAAGCTAAAC GTAAAGCATG GAAACTTAAC 
CGTGTTGGCA GCCTGCGAAA TGTTTACAGC AGCAACGCCA ACACTGAAGA ACAAGAGGAG 
AACTTTGAGT TTATTATCGT ATCCCTTACA AGTCAGAGCT GGCACTTTGA AGCTACAAGC 
TATGAGGAGA GGGATGCATG GGTACAGGCC ATAGAGAGTC AGATCTTGGC CAGCTTACAG 
TCCTGCGAAA GCAGCAAGAA CAAGTCCCGG CTCACCAGCC AGAATGAAGC TTTAGCACTA 
CAGTCGATCC GAAATCTGCC AGGAAACTCG CACTGTGTGG ACTGTGATGC ACAAAACCCA 
GATTGGGCCA GTCTGAACCT GGGTGCCCTC ATGTGCATTG AATGTTCTGG GATCCACCGT 
AACCTTGGTA CTCACCTTTC CCGTGTCCGT TCCCTGGATC TGGACGACTG GCCTCCAGAA 
CTAATTAAAG TGATGTCAGC CATCGGAAAT GAGCTGGCCA ACAGTGTGTG GGAAGGGAGC 
AGCCAAGGGC ATGTAAAGCC ATGTTCGGAG AGCCCAAGAG AAGAGAAGGA ACGCTGGATA 
CGTGCAAAGT ATGAGCAAAA GCTTTTTCTG TCACCGCTAC CCTGCCGGGA TTTACCTCTG 
GGACAGCAGC TTCTAAGGGC CACAGCAGAG GAGGATTTGA GAGCTGTAAT TTTGCTTCTT 
GCCCATGGTT CCAGGGAGGA GGTTAATGAG ACCTGCGGGG AGGGAGACAG GCGCACATCC 
CTACACCTGG CCTGTCGCAA GGGAAATGTA GTACTCGTGC AACTGCTTAT ATGGTACGGT 
GTGGATGTGA TGGCAAGGGA CTTCCATGGA AACACAGCAT TGGCGTATGC CAAACAAGCA 
GTGACGTCTG AGGTGCGGGA GTTGCTTTTG CAATATGGTT GCCCTGACGA ACAATTTGTG 
CTGATGGCCA CGCCAAACCT GTCTCGGAAA AACAACCGCA ACAACAACAG CAATGCAGGG 
GGCAGTGGCC TGATGCCCAC CTTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_002931998.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932044.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa66207
Clone ID Related Accession (Same CDS sequence)	XM_002931998.5
Accession Version	XM_002931998.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931998.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG ATCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA ATTGTAGTAG ATGGACAGAG CTACCTTCTT 
CTGATCAGGG ATGAAGGGGG ACCCCCCGAA GCACAATTTG CCATGTGGGT GGATGCAGTT 
ATCTTTGTCT TCAGCCTGGA AGATGAGATC AGCTTTCAGA CAGTCTATCA CTACTACAGC 
CGCCTTGCCA ACTACCGCAA CACCAGCGAG ATCCCTATGG TGCTGGTAGG CACTCAAGAT 
GCCATTAGCT GCAGTAACCC CCGTGTAATT GATGACAGCC GGGCACGAAA GCTTTCTAAT 
GACCTCAAGC GATGCACTTA TTATGAGACC TGCGCCACCT ATGGGCTAAA TGTGGAGCGT 
GTTTTCCAAG ATGTGGCCCA GAAGATTGTA GCCACCCGTA AGAAACAACA GCTTTCCATC 
GGACCCTGCA AGTCCCTGCC AAACTCCCCC AGCCACACGT CTGTCTGTTC TACGCAGGTT 
TCGGCTGTTC ACATTAGTCA GACCAGTAAT GGTGGGGGTA GCCTAAGCGA CTATTCCTCA 
TCTGTCCCCT CTACACCGAG TACTAGTCAG AAGGAGCTGA GGATCGACGT GCCCCCAGCA 
GCCAGCACAC CCACTCCCGT GCGCAAGCAA TCAAAACGGC GCTCCAATCT CTTCACGTCT 
CGCAAAGGGA GCGACCCAGA CAAAGATAAG AAAGGTCTAG AGAGCAGAGC AGACAGCATC 
GGCAGCGGGC GAGCAATACC AATTAAACAG GGCATGCTAC TGAAAAGAAG CGGAAAGTCT 
CTTAACAAAG AGTGGAAGAA GAAATACGTA ACCTTGTGTG ATAACGGAGT TCTGACATAT 
CATCCCAGCT TACACGATTA CATGCAAAAT GTCCATGGTA AAGAGATCGA CCTCCTTAGA 
ACTACTGTGA AGGTCCCTGG GAAAAGGCCT CCTCGAGCCA CATCCTCTTG TGCACCAGTT 
GCCAGCCCTA AAACCAATGG TCTAACCAAA GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC 
ACAGGGAACG TGACCAGCAG TACGTCAGTC ACCCAAATGG CCAGTGGTCC AAGTGGGATT 
AGTTTAGGCT CATTCAGCCG CATGGATGGG ATGCACCAGA GGTCATACTC TGTGTCTAGC 
GCTGACCAGT GGAGCGAGGG AGCCGTCATC GCCAACTCAG CCATCAGCAG CGACACTGGC 
CTAGGAGACT CAGTGTGTTC CAGTCCTAGC ATCTCCAGCA CTACAAGTCC CAAGCTAGAT 
CCGCCACCCT CGCCACACGC AAACCGCAAG AAGCACCGCC GGAAGAAAAG TACCAGTAAC 
TTTAAGGCTG ATGGGCTGTC AAGTACTGCT GAAGAACAAG AGGAGAACTT TGAGTTTATT 
ATCGTATCCC TTACAAGTCA GAGCTGGCAC TTTGAAGCTA CAAGCTATGA GGAGAGGGAT 
GCATGGGTAC AGGCCATAGA GAGTCAGATC TTGGCCAGCT TACAGTCCTG CGAAAGCAGC 
AAGAACAAGT CCCGGCTCAC CAGCCAGAAT GAAGCTTTAG CACTACAGTC GATCCGAAAT 
CTGCCAGGAA ACTCGCACTG TGTGGACTGT GATGCACAAA ACCCAGATTG GGCCAGTCTG 
AACCTGGGTG CCCTCATGTG CATTGAATGT TCTGGGATCC ACCGTAACCT TGGTACTCAC 
CTTTCCCGTG TCCGTTCCCT GGATCTGGAC GACTGGCCTC CAGAACTAAT TAAAGTGATG 
TCAGCCATCG GAAATGAGCT GGCCAACAGT GTGTGGGAAG GGAGCAGCCA AGGGCATGTA 
AAGCCATGTT CGGAGAGCCC AAGAGAAGAG AAGGAACGCT GGATACGTGC AAAGTATGAG 
CAAAAGCTTT TTCTGTCACC GCTACCCTGC CGGGATTTAC CTCTGGGACA GCAGCTTCTA 
AGGGCCACAG CAGAGGAGGA TTTGAGAGCT GTAATTTTGC TTCTTGCCCA TGGTTCCAGG 
GAGGAGGTTA ATGAGACCTG CGGGGAGGGA GACAGGCGCA CATCCCTACA CCTGGCCTGT 
CGCAAGGGAA ATGTAGTACT CGTGCAACTG CTTATATGGT ACGGTGTGGA TGTGATGGCA 
AGGGACTTCC ATGGAAACAC AGCATTGGCG TATGCCAAAC AAGCAGTGAC GTCTGAGGTG 
CGGGAGTTGC TTTTGCAATA TGGTTGCCCT GACGAACAAT TTGTGCTGAT GGCCACGCCA 
AACCTGTCTC GGAAAAACAA CCGCAACAAC AACAGCAATG CAGGGGGCAG TGGCCTGATG 
CCCACCTTAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932044.1
CDS	1229..3823
Translation	MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERLDEPALQNQIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_002931998.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931998.5

ORF Insert Sequence:

ATGAATTACC AGCAGCAGCT GGCCAACTCT GCGGCCATCC GTGCAGAGAT CCAACGTTTT 
GAATCGGTGC ACCCTAATAT CTACTCCATA TATGAGCTTC TGGAGCGTTT GGATGAACCT 
GCCCTGCAGA ACCAGATCCG AGAACATGTC ATAGCCATAG AAGATGCCTT TGTAAATAGT 
CAGGAATGGA CACTAAGCCG ATCTGTGCCA GAACTCAAAG TGGGCATTGT GGGGAACTTG 
GCCAGTGGCA AATCTGCTCT AGTGCATCGG TACCTGACTG GTACTTATGT ACAAGAGGAG 
TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA ATTGTAGTAG ATGGACAGAG CTACCTTCTT 
CTGATCAGGG ATGAAGGGGG ACCCCCCGAA GCACAATTTG CCATGTGGGT GGATGCAGTT 
ATCTTTGTCT TCAGCCTGGA AGATGAGATC AGCTTTCAGA CAGTCTATCA CTACTACAGC 
CGCCTTGCCA ACTACCGCAA CACCAGCGAG ATCCCTATGG TGCTGGTAGG CACTCAAGAT 
GCCATTAGCT GCAGTAACCC CCGTGTAATT GATGACAGCC GGGCACGAAA GCTTTCTAAT 
GACCTCAAGC GATGCACTTA TTATGAGACC TGCGCCACCT ATGGGCTAAA TGTGGAGCGT 
GTTTTCCAAG ATGTGGCCCA GAAGATTGTA GCCACCCGTA AGAAACAACA GCTTTCCATC 
GGACCCTGCA AGTCCCTGCC AAACTCCCCC AGCCACACGT CTGTCTGTTC TACGCAGGTT 
TCGGCTGTTC ACATTAGTCA GACCAGTAAT GGTGGGGGTA GCCTAAGCGA CTATTCCTCA 
TCTGTCCCCT CTACACCGAG TACTAGTCAG AAGGAGCTGA GGATCGACGT GCCCCCAGCA 
GCCAGCACAC CCACTCCCGT GCGCAAGCAA TCAAAACGGC GCTCCAATCT CTTCACGTCT 
CGCAAAGGGA GCGACCCAGA CAAAGATAAG AAAGGTCTAG AGAGCAGAGC AGACAGCATC 
GGCAGCGGGC GAGCAATACC AATTAAACAG GGCATGCTAC TGAAAAGAAG CGGAAAGTCT 
CTTAACAAAG AGTGGAAGAA GAAATACGTA ACCTTGTGTG ATAACGGAGT TCTGACATAT 
CATCCCAGCT TACACGATTA CATGCAAAAT GTCCATGGTA AAGAGATCGA CCTCCTTAGA 
ACTACTGTGA AGGTCCCTGG GAAAAGGCCT CCTCGAGCCA CATCCTCTTG TGCACCAGTT 
GCCAGCCCTA AAACCAATGG TCTAACCAAA GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC 
ACAGGGAACG TGACCAGCAG TACGTCAGTC ACCCAAATGG CCAGTGGTCC AAGTGGGATT 
AGTTTAGGCT CATTCAGCCG CATGGATGGG ATGCACCAGA GGTCATACTC TGTGTCTAGC 
GCTGACCAGT GGAGCGAGGG AGCCGTCATC GCCAACTCAG CCATCAGCAG CGACACTGGC 
CTAGGAGACT CAGTGTGTTC CAGTCCTAGC ATCTCCAGCA CTACAAGTCC CAAGCTAGAT 
CCGCCACCCT CGCCACACGC AAACCGCAAG AAGCACCGCC GGAAGAAAAG TACCAGTAAC 
TTTAAGGCTG ATGGGCTGTC AAGTACTGCT GAAGAACAAG AGGAGAACTT TGAGTTTATT 
ATCGTATCCC TTACAAGTCA GAGCTGGCAC TTTGAAGCTA CAAGCTATGA GGAGAGGGAT 
GCATGGGTAC AGGCCATAGA GAGTCAGATC TTGGCCAGCT TACAGTCCTG CGAAAGCAGC 
AAGAACAAGT CCCGGCTCAC CAGCCAGAAT GAAGCTTTAG CACTACAGTC GATCCGAAAT 
CTGCCAGGAA ACTCGCACTG TGTGGACTGT GATGCACAAA ACCCAGATTG GGCCAGTCTG 
AACCTGGGTG CCCTCATGTG CATTGAATGT TCTGGGATCC ACCGTAACCT TGGTACTCAC 
CTTTCCCGTG TCCGTTCCCT GGATCTGGAC GACTGGCCTC CAGAACTAAT TAAAGTGATG 
TCAGCCATCG GAAATGAGCT GGCCAACAGT GTGTGGGAAG GGAGCAGCCA AGGGCATGTA 
AAGCCATGTT CGGAGAGCCC AAGAGAAGAG AAGGAACGCT GGATACGTGC AAAGTATGAG 
CAAAAGCTTT TTCTGTCACC GCTACCCTGC CGGGATTTAC CTCTGGGACA GCAGCTTCTA 
AGGGCCACAG CAGAGGAGGA TTTGAGAGCT GTAATTTTGC TTCTTGCCCA TGGTTCCAGG 
GAGGAGGTTA ATGAGACCTG CGGGGAGGGA GACAGGCGCA CATCCCTACA CCTGGCCTGT 
CGCAAGGGAA ATGTAGTACT CGTGCAACTG CTTATATGGT ACGGTGTGGA TGTGATGGCA 
AGGGACTTCC ATGGAAACAC AGCATTGGCG TATGCCAAAC AAGCAGTGAC GTCTGAGGTG 
CGGGAGTTGC TTTTGCAATA TGGTTGCCCT GACGAACAAT TTGTGCTGAT GGCCACGCCA 
AACCTGTCTC GGAAAAACAA CCGCAACAAC AACAGCAATG CAGGGGGCAG TGGCCTGATG 
CCCACCTTAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917868.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917925.2 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa66202
Clone ID Related Accession (Same CDS sequence)	XM_004917868.4
Accession Version	XM_004917868.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917868.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCAGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AGAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCTGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTCATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTCTGA GAAGAGACAC CCCAGTCTTC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCTGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG ATCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG ATATGGATGC AGGTGGGAGG 
TTCAAGAAGG AAATTGTAGT AGATGGACAG AGCTACCTTC TTCTGATCAG GGATGAAGGG 
GGACCCCCCG AAGCACAATT TGCCATGTGG GTGGATGCAG TTATCTTTGT CTTCAGCCTG 
GAAGATGAGA TCAGCTTTCA GACAGTCTAT CACTACTACA GCCGCCTTGC CAACTACCGC 
AACACCAGCG AGATCCCTAT GGTGCTGGTA GGCACTCAAG ATGCCATTAG CTGCAGTAAC 
CCCCGTGTAA TTGATGACAG CCGGGCACGA AAGCTTTCTA ATGACCTCAA GCGATGCACT 
TATTATGAGA CCTGCGCCAC CTATGGGCTA AATGTGGAGC GTGTTTTCCA AGATGTGGCC 
CAGAAGATTG TAGCCACCCG TAAGAAACAA CAGCTTTCCA TCGGACCCTG CAAGTCCCTG 
CCAAACTCCC CCAGCCACAC GTCTGTCTGT TCTACGCAGG TTTCGGCTGT TCACATTAGT 
CAGACCAGTA ATGGTGGGGG TAGCCTAAGC GACTATTCCT CATCTGTCCC CTCTACACCG 
AGTACTAGTC AGAAGGAGCT GAGGATCGAC GTGCCCCCAG CAGCCAGCAC ACCCACTCCC 
GTGCGCAAGC AATCAAAACG GCGCTCCAAT CTCTTCACGT CTCGCAAAGG GAGCGACCCA 
GACAAAGATA AGAAAGGTCT AGAGAGCAGA GCAGACAGCA TCGGCAGCGG GCGAGCAATA 
CCAATTAAAC AGGGCATGCT ACTGAAAAGA AGCGGAAAGT CTCTTAACAA AGAGTGGAAG 
AAGAAATACG TAACCTTGTG TGATAACGGA GTTCTGACAT ATCATCCCAG CTTACACGAT 
TACATGCAAA ATGTCCATGG TAAAGAGATC GACCTCCTTA GAACTACTGT GAAGGTCCCT 
GGGAAAAGGC CTCCTCGAGC CACATCCTCT TGTGCACCAG TTGCCAGCCC TAAAACCAAT 
GGTCTAACCA AAGAGGTCAG CGGGCTCCAG ATTTCCCCTA ACACAGGGAA CGTGACCAGC 
AGTACGTCAG TCACCCAAAT GGCCAGTGGT CCAAGTGGGA TTAGTTTAGG CTCATTCAGC 
CGCATGGATG GGATGCACCA GAGGTCATAC TCTGTGTCTA GCGCTGACCA GTGGAGCGAG 
GGAGCCGTCA TCGCCAACTC AGCCATCAGC AGCGACACTG GCCTAGGAGA CTCAGTGTGT 
TCCAGTCCTA GCATCTCCAG CACTACAAGT CCCAAGCTAG ATCCGCCACC CTCGCCACAC 
GCAAACCGCA AGAAGCACCG CCGGAAGAAA AGTACCAGTA ACTTTAAGGC TGATGGGCTG 
TCAAGTACTG CTGAAGCTAA ACGTAAAGCA TGGAAACTTA ACCGTGTTGG CAGCCTGCGA 
AATGTTTACA GCAGCAACGC CAACACTGAA GAACAAGAGG AGAACTTTGA GTTTATTATC 
GTATCCCTTA CAAGTCAGAG CTGGCACTTT GAAGCTACAA GCTATGAGGA GAGGGATGCA 
TGGGTACAGG CCATAGAGAG TCAGATCTTG GCCAGCTTAC AGTCCTGCGA AAGCAGCAAG 
AACAAGTCCC GGCTCACCAG CCAGAATGAA GCTTTAGCAC TACAGTCGAT CCGAAATCTG 
CCAGGAAACT CGCACTGTGT GGACTGTGAT GCACAAAACC CAGATTGGGC CAGTCTGAAC 
CTGGGTGCCC TCATGTGCAT TGAATGTTCT GGGATCCACC GTAACCTTGG TACTCACCTT 
TCCCGTGTCC GTTCCCTGGA TCTGGACGAC TGGCCTCCAG AACTAATTAA AGTGATGTCA 
GCCATCGGAA ATGAGCTGGC CAACAGTGTG TGGGAAGGGA GCAGCCAAGG GCATGTAAAG 
CCATGTTCGG AGAGCCCAAG AGAAGAGAAG GAACGCTGGA TACGTGCAAA GTATGAGCAA 
AAGCTTTTTC TGTCACCGCT ACCCTGCCGG GATTTACCTC TGGGACAGCA GCTTCTAAGG 
GCCACAGCAG AGGAGGATTT GAGAGCTGTA ATTTTGCTTC TTGCCCATGG TTCCAGGGAG 
GAGGTTAATG AGACCTGCGG GGAGGGAGAC AGGCGCACAT CCCTACACCT GGCCTGTCGC 
AAGGGAAATG TAGTACTCGT GCAACTGCTT ATATGGTACG GTGTGGATGT GATGGCAAGG 
GACTTCCATG GAAACACAGC ATTGGCGTAT GCCAAACAAG CAGTGACGTC TGAGGTGCGG 
GAGTTGCTTT TGCAATATGG TTGCCCTGAC GAACAATTTG TGCTGATGGC CACGCCAAAC 
CTGTCTCGGA AAAACAACCG CAACAACAAC AGCAATGCAG GGGGCAGTGG CCTGATGCCC 
ACCTTAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917925.2
CDS	225..3836
Translation	MYLLDPAPPPMERGTPQRKTVYRISLTLVKKELLAESRLERCPEEEGEEQDTFSIHTGPRNFRTRSTGQLELGRLRVSRATSERKLGKIGGSPGDLSPGSREPWDSCAKDRVSPVVESPGSGPATERGPGVSHGVRSPQEVPKEEPCQNPLDYNNTKSPDKSIAPRTPLKSVISEDWGKAGPEGLSEKRHPSLLRRSFSFRHWNGELLRIRSLTREKHHSSSSCLTHHRADVREQMHVSATDPNAAEKRNTLDVGEVLNKSDPLTDLERWERSKSKNRTLDNSDLHKLSEQLELDRKGQERKLLRFFSGIFSRKDGSPASFAYPGGQSPRAAHSRSRGYFSSLKRPVTDNIQSSTESVNGSPYKDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEAKRKAWKLNRVGSLRNVYSSNANTEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917868.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917868.4

ORF Insert Sequence:

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCAGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AGAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCTGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTCATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTCTGA GAAGAGACAC CCCAGTCTTC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCTGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG ATCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG ATATGGATGC AGGTGGGAGG 
TTCAAGAAGG AAATTGTAGT AGATGGACAG AGCTACCTTC TTCTGATCAG GGATGAAGGG 
GGACCCCCCG AAGCACAATT TGCCATGTGG GTGGATGCAG TTATCTTTGT CTTCAGCCTG 
GAAGATGAGA TCAGCTTTCA GACAGTCTAT CACTACTACA GCCGCCTTGC CAACTACCGC 
AACACCAGCG AGATCCCTAT GGTGCTGGTA GGCACTCAAG ATGCCATTAG CTGCAGTAAC 
CCCCGTGTAA TTGATGACAG CCGGGCACGA AAGCTTTCTA ATGACCTCAA GCGATGCACT 
TATTATGAGA CCTGCGCCAC CTATGGGCTA AATGTGGAGC GTGTTTTCCA AGATGTGGCC 
CAGAAGATTG TAGCCACCCG TAAGAAACAA CAGCTTTCCA TCGGACCCTG CAAGTCCCTG 
CCAAACTCCC CCAGCCACAC GTCTGTCTGT TCTACGCAGG TTTCGGCTGT TCACATTAGT 
CAGACCAGTA ATGGTGGGGG TAGCCTAAGC GACTATTCCT CATCTGTCCC CTCTACACCG 
AGTACTAGTC AGAAGGAGCT GAGGATCGAC GTGCCCCCAG CAGCCAGCAC ACCCACTCCC 
GTGCGCAAGC AATCAAAACG GCGCTCCAAT CTCTTCACGT CTCGCAAAGG GAGCGACCCA 
GACAAAGATA AGAAAGGTCT AGAGAGCAGA GCAGACAGCA TCGGCAGCGG GCGAGCAATA 
CCAATTAAAC AGGGCATGCT ACTGAAAAGA AGCGGAAAGT CTCTTAACAA AGAGTGGAAG 
AAGAAATACG TAACCTTGTG TGATAACGGA GTTCTGACAT ATCATCCCAG CTTACACGAT 
TACATGCAAA ATGTCCATGG TAAAGAGATC GACCTCCTTA GAACTACTGT GAAGGTCCCT 
GGGAAAAGGC CTCCTCGAGC CACATCCTCT TGTGCACCAG TTGCCAGCCC TAAAACCAAT 
GGTCTAACCA AAGAGGTCAG CGGGCTCCAG ATTTCCCCTA ACACAGGGAA CGTGACCAGC 
AGTACGTCAG TCACCCAAAT GGCCAGTGGT CCAAGTGGGA TTAGTTTAGG CTCATTCAGC 
CGCATGGATG GGATGCACCA GAGGTCATAC TCTGTGTCTA GCGCTGACCA GTGGAGCGAG 
GGAGCCGTCA TCGCCAACTC AGCCATCAGC AGCGACACTG GCCTAGGAGA CTCAGTGTGT 
TCCAGTCCTA GCATCTCCAG CACTACAAGT CCCAAGCTAG ATCCGCCACC CTCGCCACAC 
GCAAACCGCA AGAAGCACCG CCGGAAGAAA AGTACCAGTA ACTTTAAGGC TGATGGGCTG 
TCAAGTACTG CTGAAGCTAA ACGTAAAGCA TGGAAACTTA ACCGTGTTGG CAGCCTGCGA 
AATGTTTACA GCAGCAACGC CAACACTGAA GAACAAGAGG AGAACTTTGA GTTTATTATC 
GTATCCCTTA CAAGTCAGAG CTGGCACTTT GAAGCTACAA GCTATGAGGA GAGGGATGCA 
TGGGTACAGG CCATAGAGAG TCAGATCTTG GCCAGCTTAC AGTCCTGCGA AAGCAGCAAG 
AACAAGTCCC GGCTCACCAG CCAGAATGAA GCTTTAGCAC TACAGTCGAT CCGAAATCTG 
CCAGGAAACT CGCACTGTGT GGACTGTGAT GCACAAAACC CAGATTGGGC CAGTCTGAAC 
CTGGGTGCCC TCATGTGCAT TGAATGTTCT GGGATCCACC GTAACCTTGG TACTCACCTT 
TCCCGTGTCC GTTCCCTGGA TCTGGACGAC TGGCCTCCAG AACTAATTAA AGTGATGTCA 
GCCATCGGAA ATGAGCTGGC CAACAGTGTG TGGGAAGGGA GCAGCCAAGG GCATGTAAAG 
CCATGTTCGG AGAGCCCAAG AGAAGAGAAG GAACGCTGGA TACGTGCAAA GTATGAGCAA 
AAGCTTTTTC TGTCACCGCT ACCCTGCCGG GATTTACCTC TGGGACAGCA GCTTCTAAGG 
GCCACAGCAG AGGAGGATTT GAGAGCTGTA ATTTTGCTTC TTGCCCATGG TTCCAGGGAG 
GAGGTTAATG AGACCTGCGG GGAGGGAGAC AGGCGCACAT CCCTACACCT GGCCTGTCGC 
AAGGGAAATG TAGTACTCGT GCAACTGCTT ATATGGTACG GTGTGGATGT GATGGCAAGG 
GACTTCCATG GAAACACAGC ATTGGCGTAT GCCAAACAAG CAGTGACGTC TGAGGTGCGG 
GAGTTGCTTT TGCAATATGG TTGCCCTGAC GAACAATTTG TGCTGATGGC CACGCCAAAC 
CTGTCTCGGA AAAACAACCG CAACAACAAC AGCAATGCAG GGGGCAGTGG CCTGATGCCC 
ACCTTAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917869.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917926.2 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa66203
Clone ID Related Accession (Same CDS sequence)	XM_004917869.4
Accession Version	XM_004917869.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917869.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCAGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AGAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCTGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTCATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTCTGA GAAGAGACAC CCCAGTCTTC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCTGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG ATCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA 
ATTGTAGTAG ATGGACAGAG CTACCTTCTT CTGATCAGGG ATGAAGGGGG ACCCCCCGAA 
GCACAATTTG CCATGTGGGT GGATGCAGTT ATCTTTGTCT TCAGCCTGGA AGATGAGATC 
AGCTTTCAGA CAGTCTATCA CTACTACAGC CGCCTTGCCA ACTACCGCAA CACCAGCGAG 
ATCCCTATGG TGCTGGTAGG CACTCAAGAT GCCATTAGCT GCAGTAACCC CCGTGTAATT 
GATGACAGCC GGGCACGAAA GCTTTCTAAT GACCTCAAGC GATGCACTTA TTATGAGACC 
TGCGCCACCT ATGGGCTAAA TGTGGAGCGT GTTTTCCAAG ATGTGGCCCA GAAGATTGTA 
GCCACCCGTA AGAAACAACA GCTTTCCATC GGACCCTGCA AGTCCCTGCC AAACTCCCCC 
AGCCACACGT CTGTCTGTTC TACGCAGGTT TCGGCTGTTC ACATTAGTCA GACCAGTAAT 
GGTGGGGGTA GCCTAAGCGA CTATTCCTCA TCTGTCCCCT CTACACCGAG TACTAGTCAG 
AAGGAGCTGA GGATCGACGT GCCCCCAGCA GCCAGCACAC CCACTCCCGT GCGCAAGCAA 
TCAAAACGGC GCTCCAATCT CTTCACGTCT CGCAAAGGGA GCGACCCAGA CAAAGATAAG 
AAAGGTCTAG AGAGCAGAGC AGACAGCATC GGCAGCGGGC GAGCAATACC AATTAAACAG 
GGCATGCTAC TGAAAAGAAG CGGAAAGTCT CTTAACAAAG AGTGGAAGAA GAAATACGTA 
ACCTTGTGTG ATAACGGAGT TCTGACATAT CATCCCAGCT TACACGATTA CATGCAAAAT 
GTCCATGGTA AAGAGATCGA CCTCCTTAGA ACTACTGTGA AGGTCCCTGG GAAAAGGCCT 
CCTCGAGCCA CATCCTCTTG TGCACCAGTT GCCAGCCCTA AAACCAATGG TCTAACCAAA 
GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC ACAGGGAACG TGACCAGCAG TACGTCAGTC 
ACCCAAATGG CCAGTGGTCC AAGTGGGATT AGTTTAGGCT CATTCAGCCG CATGGATGGG 
ATGCACCAGA GGTCATACTC TGTGTCTAGC GCTGACCAGT GGAGCGAGGG AGCCGTCATC 
GCCAACTCAG CCATCAGCAG CGACACTGGC CTAGGAGACT CAGTGTGTTC CAGTCCTAGC 
ATCTCCAGCA CTACAAGTCC CAAGCTAGAT CCGCCACCCT CGCCACACGC AAACCGCAAG 
AAGCACCGCC GGAAGAAAAG TACCAGTAAC TTTAAGGCTG ATGGGCTGTC AAGTACTGCT 
GAAGCTAAAC GTAAAGCATG GAAACTTAAC CGTGTTGGCA GCCTGCGAAA TGTTTACAGC 
AGCAACGCCA ACACTGAAGA ACAAGAGGAG AACTTTGAGT TTATTATCGT ATCCCTTACA 
AGTCAGAGCT GGCACTTTGA AGCTACAAGC TATGAGGAGA GGGATGCATG GGTACAGGCC 
ATAGAGAGTC AGATCTTGGC CAGCTTACAG TCCTGCGAAA GCAGCAAGAA CAAGTCCCGG 
CTCACCAGCC AGAATGAAGC TTTAGCACTA CAGTCGATCC GAAATCTGCC AGGAAACTCG 
CACTGTGTGG ACTGTGATGC ACAAAACCCA GATTGGGCCA GTCTGAACCT GGGTGCCCTC 
ATGTGCATTG AATGTTCTGG GATCCACCGT AACCTTGGTA CTCACCTTTC CCGTGTCCGT 
TCCCTGGATC TGGACGACTG GCCTCCAGAA CTAATTAAAG TGATGTCAGC CATCGGAAAT 
GAGCTGGCCA ACAGTGTGTG GGAAGGGAGC AGCCAAGGGC ATGTAAAGCC ATGTTCGGAG 
AGCCCAAGAG AAGAGAAGGA ACGCTGGATA CGTGCAAAGT ATGAGCAAAA GCTTTTTCTG 
TCACCGCTAC CCTGCCGGGA TTTACCTCTG GGACAGCAGC TTCTAAGGGC CACAGCAGAG 
GAGGATTTGA GAGCTGTAAT TTTGCTTCTT GCCCATGGTT CCAGGGAGGA GGTTAATGAG 
ACCTGCGGGG AGGGAGACAG GCGCACATCC CTACACCTGG CCTGTCGCAA GGGAAATGTA 
GTACTCGTGC AACTGCTTAT ATGGTACGGT GTGGATGTGA TGGCAAGGGA CTTCCATGGA 
AACACAGCAT TGGCGTATGC CAAACAAGCA GTGACGTCTG AGGTGCGGGA GTTGCTTTTG 
CAATATGGTT GCCCTGACGA ACAATTTGTG CTGATGGCCA CGCCAAACCT GTCTCGGAAA 
AACAACCGCA ACAACAACAG CAATGCAGGG GGCAGTGGCC TGATGCCCAC CTTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917926.2
CDS	225..3824
Translation	MYLLDPAPPPMERGTPQRKTVYRISLTLVKKELLAESRLERCPEEEGEEQDTFSIHTGPRNFRTRSTGQLELGRLRVSRATSERKLGKIGGSPGDLSPGSREPWDSCAKDRVSPVVESPGSGPATERGPGVSHGVRSPQEVPKEEPCQNPLDYNNTKSPDKSIAPRTPLKSVISEDWGKAGPEGLSEKRHPSLLRRSFSFRHWNGELLRIRSLTREKHHSSSSCLTHHRADVREQMHVSATDPNAAEKRNTLDVGEVLNKSDPLTDLERWERSKSKNRTLDNSDLHKLSEQLELDRKGQERKLLRFFSGIFSRKDGSPASFAYPGGQSPRAAHSRSRGYFSSLKRPVTDNIQSSTESVNGSPYKDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEAKRKAWKLNRVGSLRNVYSSNANTEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917869.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917869.4

ORF Insert Sequence:

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCAGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AGAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCTGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTCATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTCTGA GAAGAGACAC CCCAGTCTTC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCTGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG ATCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA 
ATTGTAGTAG ATGGACAGAG CTACCTTCTT CTGATCAGGG ATGAAGGGGG ACCCCCCGAA 
GCACAATTTG CCATGTGGGT GGATGCAGTT ATCTTTGTCT TCAGCCTGGA AGATGAGATC 
AGCTTTCAGA CAGTCTATCA CTACTACAGC CGCCTTGCCA ACTACCGCAA CACCAGCGAG 
ATCCCTATGG TGCTGGTAGG CACTCAAGAT GCCATTAGCT GCAGTAACCC CCGTGTAATT 
GATGACAGCC GGGCACGAAA GCTTTCTAAT GACCTCAAGC GATGCACTTA TTATGAGACC 
TGCGCCACCT ATGGGCTAAA TGTGGAGCGT GTTTTCCAAG ATGTGGCCCA GAAGATTGTA 
GCCACCCGTA AGAAACAACA GCTTTCCATC GGACCCTGCA AGTCCCTGCC AAACTCCCCC 
AGCCACACGT CTGTCTGTTC TACGCAGGTT TCGGCTGTTC ACATTAGTCA GACCAGTAAT 
GGTGGGGGTA GCCTAAGCGA CTATTCCTCA TCTGTCCCCT CTACACCGAG TACTAGTCAG 
AAGGAGCTGA GGATCGACGT GCCCCCAGCA GCCAGCACAC CCACTCCCGT GCGCAAGCAA 
TCAAAACGGC GCTCCAATCT CTTCACGTCT CGCAAAGGGA GCGACCCAGA CAAAGATAAG 
AAAGGTCTAG AGAGCAGAGC AGACAGCATC GGCAGCGGGC GAGCAATACC AATTAAACAG 
GGCATGCTAC TGAAAAGAAG CGGAAAGTCT CTTAACAAAG AGTGGAAGAA GAAATACGTA 
ACCTTGTGTG ATAACGGAGT TCTGACATAT CATCCCAGCT TACACGATTA CATGCAAAAT 
GTCCATGGTA AAGAGATCGA CCTCCTTAGA ACTACTGTGA AGGTCCCTGG GAAAAGGCCT 
CCTCGAGCCA CATCCTCTTG TGCACCAGTT GCCAGCCCTA AAACCAATGG TCTAACCAAA 
GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC ACAGGGAACG TGACCAGCAG TACGTCAGTC 
ACCCAAATGG CCAGTGGTCC AAGTGGGATT AGTTTAGGCT CATTCAGCCG CATGGATGGG 
ATGCACCAGA GGTCATACTC TGTGTCTAGC GCTGACCAGT GGAGCGAGGG AGCCGTCATC 
GCCAACTCAG CCATCAGCAG CGACACTGGC CTAGGAGACT CAGTGTGTTC CAGTCCTAGC 
ATCTCCAGCA CTACAAGTCC CAAGCTAGAT CCGCCACCCT CGCCACACGC AAACCGCAAG 
AAGCACCGCC GGAAGAAAAG TACCAGTAAC TTTAAGGCTG ATGGGCTGTC AAGTACTGCT 
GAAGCTAAAC GTAAAGCATG GAAACTTAAC CGTGTTGGCA GCCTGCGAAA TGTTTACAGC 
AGCAACGCCA ACACTGAAGA ACAAGAGGAG AACTTTGAGT TTATTATCGT ATCCCTTACA 
AGTCAGAGCT GGCACTTTGA AGCTACAAGC TATGAGGAGA GGGATGCATG GGTACAGGCC 
ATAGAGAGTC AGATCTTGGC CAGCTTACAG TCCTGCGAAA GCAGCAAGAA CAAGTCCCGG 
CTCACCAGCC AGAATGAAGC TTTAGCACTA CAGTCGATCC GAAATCTGCC AGGAAACTCG 
CACTGTGTGG ACTGTGATGC ACAAAACCCA GATTGGGCCA GTCTGAACCT GGGTGCCCTC 
ATGTGCATTG AATGTTCTGG GATCCACCGT AACCTTGGTA CTCACCTTTC CCGTGTCCGT 
TCCCTGGATC TGGACGACTG GCCTCCAGAA CTAATTAAAG TGATGTCAGC CATCGGAAAT 
GAGCTGGCCA ACAGTGTGTG GGAAGGGAGC AGCCAAGGGC ATGTAAAGCC ATGTTCGGAG 
AGCCCAAGAG AAGAGAAGGA ACGCTGGATA CGTGCAAAGT ATGAGCAAAA GCTTTTTCTG 
TCACCGCTAC CCTGCCGGGA TTTACCTCTG GGACAGCAGC TTCTAAGGGC CACAGCAGAG 
GAGGATTTGA GAGCTGTAAT TTTGCTTCTT GCCCATGGTT CCAGGGAGGA GGTTAATGAG 
ACCTGCGGGG AGGGAGACAG GCGCACATCC CTACACCTGG CCTGTCGCAA GGGAAATGTA 
GTACTCGTGC AACTGCTTAT ATGGTACGGT GTGGATGTGA TGGCAAGGGA CTTCCATGGA 
AACACAGCAT TGGCGTATGC CAAACAAGCA GTGACGTCTG AGGTGCGGGA GTTGCTTTTG 
CAATATGGTT GCCCTGACGA ACAATTTGTG CTGATGGCCA CGCCAAACCT GTCTCGGAAA 
AACAACCGCA ACAACAACAG CAATGCAGGG GGCAGTGGCC TGATGCCCAC CTTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917870.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917927.2 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X3, mRNA.

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CloneID	OXa66204
Clone ID Related Accession (Same CDS sequence)	XM_004917870.4
Accession Version	XM_004917870.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917870.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCAGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AGAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCTGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTCATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTCTGA GAAGAGACAC CCCAGTCTTC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCTGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG ATCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG ATATGGATGC AGGTGGGAGG 
TTCAAGAAGG AAATTGTAGT AGATGGACAG AGCTACCTTC TTCTGATCAG GGATGAAGGG 
GGACCCCCCG AAGCACAATT TGCCATGTGG GTGGATGCAG TTATCTTTGT CTTCAGCCTG 
GAAGATGAGA TCAGCTTTCA GACAGTCTAT CACTACTACA GCCGCCTTGC CAACTACCGC 
AACACCAGCG AGATCCCTAT GGTGCTGGTA GGCACTCAAG ATGCCATTAG CTGCAGTAAC 
CCCCGTGTAA TTGATGACAG CCGGGCACGA AAGCTTTCTA ATGACCTCAA GCGATGCACT 
TATTATGAGA CCTGCGCCAC CTATGGGCTA AATGTGGAGC GTGTTTTCCA AGATGTGGCC 
CAGAAGATTG TAGCCACCCG TAAGAAACAA CAGCTTTCCA TCGGACCCTG CAAGTCCCTG 
CCAAACTCCC CCAGCCACAC GTCTGTCTGT TCTACGCAGG TTTCGGCTGT TCACATTAGT 
CAGACCAGTA ATGGTGGGGG TAGCCTAAGC GACTATTCCT CATCTGTCCC CTCTACACCG 
AGTACTAGTC AGAAGGAGCT GAGGATCGAC GTGCCCCCAG CAGCCAGCAC ACCCACTCCC 
GTGCGCAAGC AATCAAAACG GCGCTCCAAT CTCTTCACGT CTCGCAAAGG GAGCGACCCA 
GACAAAGATA AGAAAGGTCT AGAGAGCAGA GCAGACAGCA TCGGCAGCGG GCGAGCAATA 
CCAATTAAAC AGGGCATGCT ACTGAAAAGA AGCGGAAAGT CTCTTAACAA AGAGTGGAAG 
AAGAAATACG TAACCTTGTG TGATAACGGA GTTCTGACAT ATCATCCCAG CTTACACGAT 
TACATGCAAA ATGTCCATGG TAAAGAGATC GACCTCCTTA GAACTACTGT GAAGGTCCCT 
GGGAAAAGGC CTCCTCGAGC CACATCCTCT TGTGCACCAG TTGCCAGCCC TAAAACCAAT 
GGTCTAACCA AAGAGGTCAG CGGGCTCCAG ATTTCCCCTA ACACAGGGAA CGTGACCAGC 
AGTACGTCAG TCACCCAAAT GGCCAGTGGT CCAAGTGGGA TTAGTTTAGG CTCATTCAGC 
CGCATGGATG GGATGCACCA GAGGTCATAC TCTGTGTCTA GCGCTGACCA GTGGAGCGAG 
GGAGCCGTCA TCGCCAACTC AGCCATCAGC AGCGACACTG GCCTAGGAGA CTCAGTGTGT 
TCCAGTCCTA GCATCTCCAG CACTACAAGT CCCAAGCTAG ATCCGCCACC CTCGCCACAC 
GCAAACCGCA AGAAGCACCG CCGGAAGAAA AGTACCAGTA ACTTTAAGGC TGATGGGCTG 
TCAAGTACTG CTGAAGAACA AGAGGAGAAC TTTGAGTTTA TTATCGTATC CCTTACAAGT 
CAGAGCTGGC ACTTTGAAGC TACAAGCTAT GAGGAGAGGG ATGCATGGGT ACAGGCCATA 
GAGAGTCAGA TCTTGGCCAG CTTACAGTCC TGCGAAAGCA GCAAGAACAA GTCCCGGCTC 
ACCAGCCAGA ATGAAGCTTT AGCACTACAG TCGATCCGAA ATCTGCCAGG AAACTCGCAC 
TGTGTGGACT GTGATGCACA AAACCCAGAT TGGGCCAGTC TGAACCTGGG TGCCCTCATG 
TGCATTGAAT GTTCTGGGAT CCACCGTAAC CTTGGTACTC ACCTTTCCCG TGTCCGTTCC 
CTGGATCTGG ACGACTGGCC TCCAGAACTA ATTAAAGTGA TGTCAGCCAT CGGAAATGAG 
CTGGCCAACA GTGTGTGGGA AGGGAGCAGC CAAGGGCATG TAAAGCCATG TTCGGAGAGC 
CCAAGAGAAG AGAAGGAACG CTGGATACGT GCAAAGTATG AGCAAAAGCT TTTTCTGTCA 
CCGCTACCCT GCCGGGATTT ACCTCTGGGA CAGCAGCTTC TAAGGGCCAC AGCAGAGGAG 
GATTTGAGAG CTGTAATTTT GCTTCTTGCC CATGGTTCCA GGGAGGAGGT TAATGAGACC 
TGCGGGGAGG GAGACAGGCG CACATCCCTA CACCTGGCCT GTCGCAAGGG AAATGTAGTA 
CTCGTGCAAC TGCTTATATG GTACGGTGTG GATGTGATGG CAAGGGACTT CCATGGAAAC 
ACAGCATTGG CGTATGCCAA ACAAGCAGTG ACGTCTGAGG TGCGGGAGTT GCTTTTGCAA 
TATGGTTGCC CTGACGAACA ATTTGTGCTG ATGGCCACGC CAAACCTGTC TCGGAAAAAC 
AACCGCAACA ACAACAGCAA TGCAGGGGGC AGTGGCCTGA TGCCCACCTT AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917927.2
CDS	224..3760
Translation	MYLLDPAPPPMERGTPQRKTVYRISLTLVKKELLAESRLERCPEEEGEEQDTFSIHTGPRNFRTRSTGQLELGRLRVSRATSERKLGKIGGSPGDLSPGSREPWDSCAKDRVSPVVESPGSGPATERGPGVSHGVRSPQEVPKEEPCQNPLDYNNTKSPDKSIAPRTPLKSVISEDWGKAGPEGLSEKRHPSLLRRSFSFRHWNGELLRIRSLTREKHHSSSSCLTHHRADVREQMHVSATDPNAAEKRNTLDVGEVLNKSDPLTDLERWERSKSKNRTLDNSDLHKLSEQLELDRKGQERKLLRFFSGIFSRKDGSPASFAYPGGQSPRAAHSRSRGYFSSLKRPVTDNIQSSTESVNGSPYKDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917870.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917870.4

ORF Insert Sequence:

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCAGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AGAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCTGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTCATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTCTGA GAAGAGACAC CCCAGTCTTC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCTGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG ATCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG ATATGGATGC AGGTGGGAGG 
TTCAAGAAGG AAATTGTAGT AGATGGACAG AGCTACCTTC TTCTGATCAG GGATGAAGGG 
GGACCCCCCG AAGCACAATT TGCCATGTGG GTGGATGCAG TTATCTTTGT CTTCAGCCTG 
GAAGATGAGA TCAGCTTTCA GACAGTCTAT CACTACTACA GCCGCCTTGC CAACTACCGC 
AACACCAGCG AGATCCCTAT GGTGCTGGTA GGCACTCAAG ATGCCATTAG CTGCAGTAAC 
CCCCGTGTAA TTGATGACAG CCGGGCACGA AAGCTTTCTA ATGACCTCAA GCGATGCACT 
TATTATGAGA CCTGCGCCAC CTATGGGCTA AATGTGGAGC GTGTTTTCCA AGATGTGGCC 
CAGAAGATTG TAGCCACCCG TAAGAAACAA CAGCTTTCCA TCGGACCCTG CAAGTCCCTG 
CCAAACTCCC CCAGCCACAC GTCTGTCTGT TCTACGCAGG TTTCGGCTGT TCACATTAGT 
CAGACCAGTA ATGGTGGGGG TAGCCTAAGC GACTATTCCT CATCTGTCCC CTCTACACCG 
AGTACTAGTC AGAAGGAGCT GAGGATCGAC GTGCCCCCAG CAGCCAGCAC ACCCACTCCC 
GTGCGCAAGC AATCAAAACG GCGCTCCAAT CTCTTCACGT CTCGCAAAGG GAGCGACCCA 
GACAAAGATA AGAAAGGTCT AGAGAGCAGA GCAGACAGCA TCGGCAGCGG GCGAGCAATA 
CCAATTAAAC AGGGCATGCT ACTGAAAAGA AGCGGAAAGT CTCTTAACAA AGAGTGGAAG 
AAGAAATACG TAACCTTGTG TGATAACGGA GTTCTGACAT ATCATCCCAG CTTACACGAT 
TACATGCAAA ATGTCCATGG TAAAGAGATC GACCTCCTTA GAACTACTGT GAAGGTCCCT 
GGGAAAAGGC CTCCTCGAGC CACATCCTCT TGTGCACCAG TTGCCAGCCC TAAAACCAAT 
GGTCTAACCA AAGAGGTCAG CGGGCTCCAG ATTTCCCCTA ACACAGGGAA CGTGACCAGC 
AGTACGTCAG TCACCCAAAT GGCCAGTGGT CCAAGTGGGA TTAGTTTAGG CTCATTCAGC 
CGCATGGATG GGATGCACCA GAGGTCATAC TCTGTGTCTA GCGCTGACCA GTGGAGCGAG 
GGAGCCGTCA TCGCCAACTC AGCCATCAGC AGCGACACTG GCCTAGGAGA CTCAGTGTGT 
TCCAGTCCTA GCATCTCCAG CACTACAAGT CCCAAGCTAG ATCCGCCACC CTCGCCACAC 
GCAAACCGCA AGAAGCACCG CCGGAAGAAA AGTACCAGTA ACTTTAAGGC TGATGGGCTG 
TCAAGTACTG CTGAAGAACA AGAGGAGAAC TTTGAGTTTA TTATCGTATC CCTTACAAGT 
CAGAGCTGGC ACTTTGAAGC TACAAGCTAT GAGGAGAGGG ATGCATGGGT ACAGGCCATA 
GAGAGTCAGA TCTTGGCCAG CTTACAGTCC TGCGAAAGCA GCAAGAACAA GTCCCGGCTC 
ACCAGCCAGA ATGAAGCTTT AGCACTACAG TCGATCCGAA ATCTGCCAGG AAACTCGCAC 
TGTGTGGACT GTGATGCACA AAACCCAGAT TGGGCCAGTC TGAACCTGGG TGCCCTCATG 
TGCATTGAAT GTTCTGGGAT CCACCGTAAC CTTGGTACTC ACCTTTCCCG TGTCCGTTCC 
CTGGATCTGG ACGACTGGCC TCCAGAACTA ATTAAAGTGA TGTCAGCCAT CGGAAATGAG 
CTGGCCAACA GTGTGTGGGA AGGGAGCAGC CAAGGGCATG TAAAGCCATG TTCGGAGAGC 
CCAAGAGAAG AGAAGGAACG CTGGATACGT GCAAAGTATG AGCAAAAGCT TTTTCTGTCA 
CCGCTACCCT GCCGGGATTT ACCTCTGGGA CAGCAGCTTC TAAGGGCCAC AGCAGAGGAG 
GATTTGAGAG CTGTAATTTT GCTTCTTGCC CATGGTTCCA GGGAGGAGGT TAATGAGACC 
TGCGGGGAGG GAGACAGGCG CACATCCCTA CACCTGGCCT GTCGCAAGGG AAATGTAGTA 
CTCGTGCAAC TGCTTATATG GTACGGTGTG GATGTGATGG CAAGGGACTT CCATGGAAAC 
ACAGCATTGG CGTATGCCAA ACAAGCAGTG ACGTCTGAGG TGCGGGAGTT GCTTTTGCAA 
TATGGTTGCC CTGACGAACA ATTTGTGCTG ATGGCCACGC CAAACCTGTC TCGGAAAAAC 
AACCGCAACA ACAACAGCAA TGCAGGGGGC AGTGGCCTGA TGCCCACCTT AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917868.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917925.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16167
Clone ID Related Accession (Same CDS sequence)	XM_004917868.3
Accession Version	XM_004917868.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917868.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCCGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AAAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCCGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTAATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTGTGA GAAGAGACAC CCCAGTCTCC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCGGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG TTCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG ATATGGATGC AGGTGGGAGG 
TTCAAGAAGG AAATTGTAGT AGATGGACAG AGCTACCTTC TTCTGATCAG GGATGAAGGG 
GGACCCCCCG AAGCACAATT TGCCATGTGG GTGGATGCAG TTATCTTTGT CTTCAGCCTG 
GAAGATGAGA TCAGCTTTCA GACAGTCTAT CACTACTACA GCCGCCTTGC CAACTACCGC 
AACACCAGCG AGATCCCTAT GGTGCTGGTA GGCACTCAAG ATGCCATTAG CTGCAGTAAC 
CCCCGTGTAA TTGATGACAG CCGGGCACGA AAGCTTTCTA ATGACCTCAA GCGATGCACT 
TATTATGAGA CCTGCGCCAC CTATGGGCTA AATGTGGAGC GTGTTTTCCA AGATGTGGCC 
CAGAAGATTG TAGCCACCCG TAAGAAACAA CAGCTTTCCA TCGGACCCTG CAAGTCCCTG 
CCAAACTCCC CCAGCCACAC GTCTGTCTGT TCTACGCAGG TTTCGGCTGT TCACATTAGT 
CAGACCAGTA ATGGTGGGGG TAGCCTAAGC GACTATTCCT CATCTGTCCC CTCTACACCG 
AGTACTAGTC AGAAGGAGCT GAGGATCGAC GTGCCCCCAG CAGCCAGCAC ACCCACTCCC 
GTGCGCAAGC AATCAAAACG GCGCTCCAAT CTCTTCACGT CTCGCAAAGG GAGCGACCCA 
GACAAAGATA AGAAAGGTCT AGAGAGCAGA GCAGACAGCA TCGGCAGCGG GCGAGCAATA 
CCAATTAAAC AGGGCATGCT ACTGAAAAGA AGCGGAAAGT CTCTTAACAA AGAGTGGAAG 
AAGAAATACG TAACCTTGTG TGATAACGGA GTTCTGACAT ATCATCCCAG CTTACACGAT 
TACATGCAAA ATGTCCATGG TAAAGAGATC GACCTCCTTA GAACTACTGT GAAGGTCCCT 
GGGAAAAGGC CTCCTCGAGC CACATCCTCT TGTGCACCAG TTGCCAGCCC TAAAACCAAT 
GGTCTAACCA AAGAGGTCAG CGGGCTCCAG ATTTCCCCTA ACACAGGGAA CGTGACCAGC 
AGTACGTCAG TCACCCAAAT GGCCAGTGGT CCAAGTGGGA TTAGTTTAGG CTCATTCAGC 
CGCATGGATG GGATGCACCA GAGGTCATAC TCTGTGTCTA GCGCTGACCA GTGGAGCGAG 
GGAGCCGTCA TCGCCAACTC AGCCATCAGC AGCGACACTG GCCTAGGAGA CTCAGTGTGT 
TCCAGTCCTA GCATCTCCAG CACTACAAGT CCCAAGCTAG ATCCGCCACC CTCGCCACAT 
GCAAACCGCA AGAAGCACCG CCGGAAGAAA AGTACCAGTA ACTTTAAGGC TGATGGGCTG 
TCAAGTACTG CTGAAGCTAA ACGTAAAGCA TGGAAACTTA ACCGTGTTGG CAGCCTGCGA 
AATGTTTACA GCAGCAACGC CAACACTGAA GAACAAGAGG AGAACTTTGA GTTTATTATC 
GTATCCCTTA CAAGTCAGAG CTGGCACTTT GAAGCTACAA GCTATGAGGA GAGGGATGCA 
TGGGTACAGG CCATAGAGAG TCAGATCTTG GCCAGCTTAC AGTCCTGCGA AAGCAGCAAG 
AACAAGTCCC GGCTCACCAG CCAGAATGAA GCTTTAGCAC TACAGTCGAT CCGAAATCTG 
CCAGGAAACT CGCACTGTGT GGACTGTGAT GCACAAAACC CAGATTGGGC CAGTCTGAAC 
CTGGGTGCCC TCATGTGCAT TGAATGTTCT GGGATCCACC GTAACCTTGG TACTCACCTT 
TCCCGTGTCC GTTCCCTGGA TCTGGACGAC TGGCCTCCAG AACTAATTAA AGTGATGTCA 
GCCATCGGAA ATGAGCTGGC CAACAGTGTG TGGGAAGGGA GCAGCCAAGG GCATGTAAAG 
CCATGTTCGG AGAGCCCAAG AGAAGAGAAG GAACGCTGGA TACGTGCAAA GTATGAGCAA 
AAGCTTTTTC TGTCACCGCT ACCCTGCCGG GATTTACCTC TGGGACAGCA GCTTCTAAGG 
GCCACAGCAG AGGAGGATTT GAGAGCTGTA ATTTTGCTTC TTGCCCATGG TTCCAGGGAG 
GAGGTTAATG AGACCTGCGG GGAGGGAGAC AGGCGCACAT CCCTACACCT GGCCTGTCGC 
AAGGGAAATG TAGTACTCGT GCAACTGCTT ATATGGTACG GTGTGGATGT GATGGCAAGG 
GACTTCCATG GAAACACAGC ATTGGCGTAT GCCAAACAAG CAGTGACGTC TGAGGTGCGG 
GAGTTGCTTT TGCAATATGG TTGCCCTGAC GAACAATTTG TGCTGATGGC CACGCCAAAC 
CTGTCTCGGA AAAACAACCG CAACAACAAC AGCAATGCAG GGGGCAGTGG CCTGATGCCC 
ACCTTAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917925.1
CDS	227..3838
Translation	MYLLDPAPPPMERGTPQRKTVYRISLTLVKKELLAESRLERCPEEEGEEQDTFSIHTGPRNFRTRSTGQLELGRLRVSRATSERKLGKIGGSPGDLSPGSREPWDSCAKDKVSPVVESPGSGPATERGPGVSHGVRSPQEVPKEEPCQNPLDYNNTKSPDKSIAPRTPLKSVISEDWGKAGPEGLCEKRHPSLLRRSFSFRHWNGELLRIRSLTREKHHSSSSCLTHHRADVREQMHVSATDPNAAEKRNTLDVGEVLNKSDPLTDLERWERSKSKNRTLDNSDLHKLSEQLELDRKGQERKLLRFFSGIFSRKDGSPASFAYPGGQSPRAAHSRSRGYFSSLKRPVTDNIQSSTESVNGSPYKDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEAKRKAWKLNRVGSLRNVYSSNANTEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917868.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917868.3

ORF Insert Sequence:

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCCGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AAAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCCGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTAATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTGTGA GAAGAGACAC CCCAGTCTCC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCGGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG TTCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG ATATGGATGC AGGTGGGAGG 
TTCAAGAAGG AAATTGTAGT AGATGGACAG AGCTACCTTC TTCTGATCAG GGATGAAGGG 
GGACCCCCCG AAGCACAATT TGCCATGTGG GTGGATGCAG TTATCTTTGT CTTCAGCCTG 
GAAGATGAGA TCAGCTTTCA GACAGTCTAT CACTACTACA GCCGCCTTGC CAACTACCGC 
AACACCAGCG AGATCCCTAT GGTGCTGGTA GGCACTCAAG ATGCCATTAG CTGCAGTAAC 
CCCCGTGTAA TTGATGACAG CCGGGCACGA AAGCTTTCTA ATGACCTCAA GCGATGCACT 
TATTATGAGA CCTGCGCCAC CTATGGGCTA AATGTGGAGC GTGTTTTCCA AGATGTGGCC 
CAGAAGATTG TAGCCACCCG TAAGAAACAA CAGCTTTCCA TCGGACCCTG CAAGTCCCTG 
CCAAACTCCC CCAGCCACAC GTCTGTCTGT TCTACGCAGG TTTCGGCTGT TCACATTAGT 
CAGACCAGTA ATGGTGGGGG TAGCCTAAGC GACTATTCCT CATCTGTCCC CTCTACACCG 
AGTACTAGTC AGAAGGAGCT GAGGATCGAC GTGCCCCCAG CAGCCAGCAC ACCCACTCCC 
GTGCGCAAGC AATCAAAACG GCGCTCCAAT CTCTTCACGT CTCGCAAAGG GAGCGACCCA 
GACAAAGATA AGAAAGGTCT AGAGAGCAGA GCAGACAGCA TCGGCAGCGG GCGAGCAATA 
CCAATTAAAC AGGGCATGCT ACTGAAAAGA AGCGGAAAGT CTCTTAACAA AGAGTGGAAG 
AAGAAATACG TAACCTTGTG TGATAACGGA GTTCTGACAT ATCATCCCAG CTTACACGAT 
TACATGCAAA ATGTCCATGG TAAAGAGATC GACCTCCTTA GAACTACTGT GAAGGTCCCT 
GGGAAAAGGC CTCCTCGAGC CACATCCTCT TGTGCACCAG TTGCCAGCCC TAAAACCAAT 
GGTCTAACCA AAGAGGTCAG CGGGCTCCAG ATTTCCCCTA ACACAGGGAA CGTGACCAGC 
AGTACGTCAG TCACCCAAAT GGCCAGTGGT CCAAGTGGGA TTAGTTTAGG CTCATTCAGC 
CGCATGGATG GGATGCACCA GAGGTCATAC TCTGTGTCTA GCGCTGACCA GTGGAGCGAG 
GGAGCCGTCA TCGCCAACTC AGCCATCAGC AGCGACACTG GCCTAGGAGA CTCAGTGTGT 
TCCAGTCCTA GCATCTCCAG CACTACAAGT CCCAAGCTAG ATCCGCCACC CTCGCCACAT 
GCAAACCGCA AGAAGCACCG CCGGAAGAAA AGTACCAGTA ACTTTAAGGC TGATGGGCTG 
TCAAGTACTG CTGAAGCTAA ACGTAAAGCA TGGAAACTTA ACCGTGTTGG CAGCCTGCGA 
AATGTTTACA GCAGCAACGC CAACACTGAA GAACAAGAGG AGAACTTTGA GTTTATTATC 
GTATCCCTTA CAAGTCAGAG CTGGCACTTT GAAGCTACAA GCTATGAGGA GAGGGATGCA 
TGGGTACAGG CCATAGAGAG TCAGATCTTG GCCAGCTTAC AGTCCTGCGA AAGCAGCAAG 
AACAAGTCCC GGCTCACCAG CCAGAATGAA GCTTTAGCAC TACAGTCGAT CCGAAATCTG 
CCAGGAAACT CGCACTGTGT GGACTGTGAT GCACAAAACC CAGATTGGGC CAGTCTGAAC 
CTGGGTGCCC TCATGTGCAT TGAATGTTCT GGGATCCACC GTAACCTTGG TACTCACCTT 
TCCCGTGTCC GTTCCCTGGA TCTGGACGAC TGGCCTCCAG AACTAATTAA AGTGATGTCA 
GCCATCGGAA ATGAGCTGGC CAACAGTGTG TGGGAAGGGA GCAGCCAAGG GCATGTAAAG 
CCATGTTCGG AGAGCCCAAG AGAAGAGAAG GAACGCTGGA TACGTGCAAA GTATGAGCAA 
AAGCTTTTTC TGTCACCGCT ACCCTGCCGG GATTTACCTC TGGGACAGCA GCTTCTAAGG 
GCCACAGCAG AGGAGGATTT GAGAGCTGTA ATTTTGCTTC TTGCCCATGG TTCCAGGGAG 
GAGGTTAATG AGACCTGCGG GGAGGGAGAC AGGCGCACAT CCCTACACCT GGCCTGTCGC 
AAGGGAAATG TAGTACTCGT GCAACTGCTT ATATGGTACG GTGTGGATGT GATGGCAAGG 
GACTTCCATG GAAACACAGC ATTGGCGTAT GCCAAACAAG CAGTGACGTC TGAGGTGCGG 
GAGTTGCTTT TGCAATATGG TTGCCCTGAC GAACAATTTG TGCTGATGGC CACGCCAAAC 
CTGTCTCGGA AAAACAACCG CAACAACAAC AGCAATGCAG GGGGCAGTGG CCTGATGCCC 
ACCTTAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917869.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917926.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16168
Clone ID Related Accession (Same CDS sequence)	XM_004917869.3
Accession Version	XM_004917869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCCGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AAAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCCGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTAATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTGTGA GAAGAGACAC CCCAGTCTCC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCGGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG TTCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA 
ATTGTAGTAG ATGGACAGAG CTACCTTCTT CTGATCAGGG ATGAAGGGGG ACCCCCCGAA 
GCACAATTTG CCATGTGGGT GGATGCAGTT ATCTTTGTCT TCAGCCTGGA AGATGAGATC 
AGCTTTCAGA CAGTCTATCA CTACTACAGC CGCCTTGCCA ACTACCGCAA CACCAGCGAG 
ATCCCTATGG TGCTGGTAGG CACTCAAGAT GCCATTAGCT GCAGTAACCC CCGTGTAATT 
GATGACAGCC GGGCACGAAA GCTTTCTAAT GACCTCAAGC GATGCACTTA TTATGAGACC 
TGCGCCACCT ATGGGCTAAA TGTGGAGCGT GTTTTCCAAG ATGTGGCCCA GAAGATTGTA 
GCCACCCGTA AGAAACAACA GCTTTCCATC GGACCCTGCA AGTCCCTGCC AAACTCCCCC 
AGCCACACGT CTGTCTGTTC TACGCAGGTT TCGGCTGTTC ACATTAGTCA GACCAGTAAT 
GGTGGGGGTA GCCTAAGCGA CTATTCCTCA TCTGTCCCCT CTACACCGAG TACTAGTCAG 
AAGGAGCTGA GGATCGACGT GCCCCCAGCA GCCAGCACAC CCACTCCCGT GCGCAAGCAA 
TCAAAACGGC GCTCCAATCT CTTCACGTCT CGCAAAGGGA GCGACCCAGA CAAAGATAAG 
AAAGGTCTAG AGAGCAGAGC AGACAGCATC GGCAGCGGGC GAGCAATACC AATTAAACAG 
GGCATGCTAC TGAAAAGAAG CGGAAAGTCT CTTAACAAAG AGTGGAAGAA GAAATACGTA 
ACCTTGTGTG ATAACGGAGT TCTGACATAT CATCCCAGCT TACACGATTA CATGCAAAAT 
GTCCATGGTA AAGAGATCGA CCTCCTTAGA ACTACTGTGA AGGTCCCTGG GAAAAGGCCT 
CCTCGAGCCA CATCCTCTTG TGCACCAGTT GCCAGCCCTA AAACCAATGG TCTAACCAAA 
GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC ACAGGGAACG TGACCAGCAG TACGTCAGTC 
ACCCAAATGG CCAGTGGTCC AAGTGGGATT AGTTTAGGCT CATTCAGCCG CATGGATGGG 
ATGCACCAGA GGTCATACTC TGTGTCTAGC GCTGACCAGT GGAGCGAGGG AGCCGTCATC 
GCCAACTCAG CCATCAGCAG CGACACTGGC CTAGGAGACT CAGTGTGTTC CAGTCCTAGC 
ATCTCCAGCA CTACAAGTCC CAAGCTAGAT CCGCCACCCT CGCCACATGC AAACCGCAAG 
AAGCACCGCC GGAAGAAAAG TACCAGTAAC TTTAAGGCTG ATGGGCTGTC AAGTACTGCT 
GAAGCTAAAC GTAAAGCATG GAAACTTAAC CGTGTTGGCA GCCTGCGAAA TGTTTACAGC 
AGCAACGCCA ACACTGAAGA ACAAGAGGAG AACTTTGAGT TTATTATCGT ATCCCTTACA 
AGTCAGAGCT GGCACTTTGA AGCTACAAGC TATGAGGAGA GGGATGCATG GGTACAGGCC 
ATAGAGAGTC AGATCTTGGC CAGCTTACAG TCCTGCGAAA GCAGCAAGAA CAAGTCCCGG 
CTCACCAGCC AGAATGAAGC TTTAGCACTA CAGTCGATCC GAAATCTGCC AGGAAACTCG 
CACTGTGTGG ACTGTGATGC ACAAAACCCA GATTGGGCCA GTCTGAACCT GGGTGCCCTC 
ATGTGCATTG AATGTTCTGG GATCCACCGT AACCTTGGTA CTCACCTTTC CCGTGTCCGT 
TCCCTGGATC TGGACGACTG GCCTCCAGAA CTAATTAAAG TGATGTCAGC CATCGGAAAT 
GAGCTGGCCA ACAGTGTGTG GGAAGGGAGC AGCCAAGGGC ATGTAAAGCC ATGTTCGGAG 
AGCCCAAGAG AAGAGAAGGA ACGCTGGATA CGTGCAAAGT ATGAGCAAAA GCTTTTTCTG 
TCACCGCTAC CCTGCCGGGA TTTACCTCTG GGACAGCAGC TTCTAAGGGC CACAGCAGAG 
GAGGATTTGA GAGCTGTAAT TTTGCTTCTT GCCCATGGTT CCAGGGAGGA GGTTAATGAG 
ACCTGCGGGG AGGGAGACAG GCGCACATCC CTACACCTGG CCTGTCGCAA GGGAAATGTA 
GTACTCGTGC AACTGCTTAT ATGGTACGGT GTGGATGTGA TGGCAAGGGA CTTCCATGGA 
AACACAGCAT TGGCGTATGC CAAACAAGCA GTGACGTCTG AGGTGCGGGA GTTGCTTTTG 
CAATATGGTT GCCCTGACGA ACAATTTGTG CTGATGGCCA CGCCAAACCT GTCTCGGAAA 
AACAACCGCA ACAACAACAG CAATGCAGGG GGCAGTGGCC TGATGCCCAC CTTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917926.1
CDS	227..3826
Translation	MYLLDPAPPPMERGTPQRKTVYRISLTLVKKELLAESRLERCPEEEGEEQDTFSIHTGPRNFRTRSTGQLELGRLRVSRATSERKLGKIGGSPGDLSPGSREPWDSCAKDKVSPVVESPGSGPATERGPGVSHGVRSPQEVPKEEPCQNPLDYNNTKSPDKSIAPRTPLKSVISEDWGKAGPEGLCEKRHPSLLRRSFSFRHWNGELLRIRSLTREKHHSSSSCLTHHRADVREQMHVSATDPNAAEKRNTLDVGEVLNKSDPLTDLERWERSKSKNRTLDNSDLHKLSEQLELDRKGQERKLLRFFSGIFSRKDGSPASFAYPGGQSPRAAHSRSRGYFSSLKRPVTDNIQSSTESVNGSPYKDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEAKRKAWKLNRVGSLRNVYSSNANTEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917869.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917869.3

ORF Insert Sequence:

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCCGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AAAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCCGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTAATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTGTGA GAAGAGACAC CCCAGTCTCC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCGGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG TTCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG GTGGGAGGTT CAAGAAGGAA 
ATTGTAGTAG ATGGACAGAG CTACCTTCTT CTGATCAGGG ATGAAGGGGG ACCCCCCGAA 
GCACAATTTG CCATGTGGGT GGATGCAGTT ATCTTTGTCT TCAGCCTGGA AGATGAGATC 
AGCTTTCAGA CAGTCTATCA CTACTACAGC CGCCTTGCCA ACTACCGCAA CACCAGCGAG 
ATCCCTATGG TGCTGGTAGG CACTCAAGAT GCCATTAGCT GCAGTAACCC CCGTGTAATT 
GATGACAGCC GGGCACGAAA GCTTTCTAAT GACCTCAAGC GATGCACTTA TTATGAGACC 
TGCGCCACCT ATGGGCTAAA TGTGGAGCGT GTTTTCCAAG ATGTGGCCCA GAAGATTGTA 
GCCACCCGTA AGAAACAACA GCTTTCCATC GGACCCTGCA AGTCCCTGCC AAACTCCCCC 
AGCCACACGT CTGTCTGTTC TACGCAGGTT TCGGCTGTTC ACATTAGTCA GACCAGTAAT 
GGTGGGGGTA GCCTAAGCGA CTATTCCTCA TCTGTCCCCT CTACACCGAG TACTAGTCAG 
AAGGAGCTGA GGATCGACGT GCCCCCAGCA GCCAGCACAC CCACTCCCGT GCGCAAGCAA 
TCAAAACGGC GCTCCAATCT CTTCACGTCT CGCAAAGGGA GCGACCCAGA CAAAGATAAG 
AAAGGTCTAG AGAGCAGAGC AGACAGCATC GGCAGCGGGC GAGCAATACC AATTAAACAG 
GGCATGCTAC TGAAAAGAAG CGGAAAGTCT CTTAACAAAG AGTGGAAGAA GAAATACGTA 
ACCTTGTGTG ATAACGGAGT TCTGACATAT CATCCCAGCT TACACGATTA CATGCAAAAT 
GTCCATGGTA AAGAGATCGA CCTCCTTAGA ACTACTGTGA AGGTCCCTGG GAAAAGGCCT 
CCTCGAGCCA CATCCTCTTG TGCACCAGTT GCCAGCCCTA AAACCAATGG TCTAACCAAA 
GAGGTCAGCG GGCTCCAGAT TTCCCCTAAC ACAGGGAACG TGACCAGCAG TACGTCAGTC 
ACCCAAATGG CCAGTGGTCC AAGTGGGATT AGTTTAGGCT CATTCAGCCG CATGGATGGG 
ATGCACCAGA GGTCATACTC TGTGTCTAGC GCTGACCAGT GGAGCGAGGG AGCCGTCATC 
GCCAACTCAG CCATCAGCAG CGACACTGGC CTAGGAGACT CAGTGTGTTC CAGTCCTAGC 
ATCTCCAGCA CTACAAGTCC CAAGCTAGAT CCGCCACCCT CGCCACATGC AAACCGCAAG 
AAGCACCGCC GGAAGAAAAG TACCAGTAAC TTTAAGGCTG ATGGGCTGTC AAGTACTGCT 
GAAGCTAAAC GTAAAGCATG GAAACTTAAC CGTGTTGGCA GCCTGCGAAA TGTTTACAGC 
AGCAACGCCA ACACTGAAGA ACAAGAGGAG AACTTTGAGT TTATTATCGT ATCCCTTACA 
AGTCAGAGCT GGCACTTTGA AGCTACAAGC TATGAGGAGA GGGATGCATG GGTACAGGCC 
ATAGAGAGTC AGATCTTGGC CAGCTTACAG TCCTGCGAAA GCAGCAAGAA CAAGTCCCGG 
CTCACCAGCC AGAATGAAGC TTTAGCACTA CAGTCGATCC GAAATCTGCC AGGAAACTCG 
CACTGTGTGG ACTGTGATGC ACAAAACCCA GATTGGGCCA GTCTGAACCT GGGTGCCCTC 
ATGTGCATTG AATGTTCTGG GATCCACCGT AACCTTGGTA CTCACCTTTC CCGTGTCCGT 
TCCCTGGATC TGGACGACTG GCCTCCAGAA CTAATTAAAG TGATGTCAGC CATCGGAAAT 
GAGCTGGCCA ACAGTGTGTG GGAAGGGAGC AGCCAAGGGC ATGTAAAGCC ATGTTCGGAG 
AGCCCAAGAG AAGAGAAGGA ACGCTGGATA CGTGCAAAGT ATGAGCAAAA GCTTTTTCTG 
TCACCGCTAC CCTGCCGGGA TTTACCTCTG GGACAGCAGC TTCTAAGGGC CACAGCAGAG 
GAGGATTTGA GAGCTGTAAT TTTGCTTCTT GCCCATGGTT CCAGGGAGGA GGTTAATGAG 
ACCTGCGGGG AGGGAGACAG GCGCACATCC CTACACCTGG CCTGTCGCAA GGGAAATGTA 
GTACTCGTGC AACTGCTTAT ATGGTACGGT GTGGATGTGA TGGCAAGGGA CTTCCATGGA 
AACACAGCAT TGGCGTATGC CAAACAAGCA GTGACGTCTG AGGTGCGGGA GTTGCTTTTG 
CAATATGGTT GCCCTGACGA ACAATTTGTG CTGATGGCCA CGCCAAACCT GTCTCGGAAA 
AACAACCGCA ACAACAACAG CAATGCAGGG GGCAGTGGCC TGATGCCCAC CTTAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap1 ( XM_004917870.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917927.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X3, mRNA.

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CloneID	OXa16169
Clone ID Related Accession (Same CDS sequence)	XM_004917870.3
Accession Version	XM_004917870.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917870.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCCGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AAAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCCGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTAATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTGTGA GAAGAGACAC CCCAGTCTCC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCGGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG TTCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG ATATGGATGC AGGTGGGAGG 
TTCAAGAAGG AAATTGTAGT AGATGGACAG AGCTACCTTC TTCTGATCAG GGATGAAGGG 
GGACCCCCCG AAGCACAATT TGCCATGTGG GTGGATGCAG TTATCTTTGT CTTCAGCCTG 
GAAGATGAGA TCAGCTTTCA GACAGTCTAT CACTACTACA GCCGCCTTGC CAACTACCGC 
AACACCAGCG AGATCCCTAT GGTGCTGGTA GGCACTCAAG ATGCCATTAG CTGCAGTAAC 
CCCCGTGTAA TTGATGACAG CCGGGCACGA AAGCTTTCTA ATGACCTCAA GCGATGCACT 
TATTATGAGA CCTGCGCCAC CTATGGGCTA AATGTGGAGC GTGTTTTCCA AGATGTGGCC 
CAGAAGATTG TAGCCACCCG TAAGAAACAA CAGCTTTCCA TCGGACCCTG CAAGTCCCTG 
CCAAACTCCC CCAGCCACAC GTCTGTCTGT TCTACGCAGG TTTCGGCTGT TCACATTAGT 
CAGACCAGTA ATGGTGGGGG TAGCCTAAGC GACTATTCCT CATCTGTCCC CTCTACACCG 
AGTACTAGTC AGAAGGAGCT GAGGATCGAC GTGCCCCCAG CAGCCAGCAC ACCCACTCCC 
GTGCGCAAGC AATCAAAACG GCGCTCCAAT CTCTTCACGT CTCGCAAAGG GAGCGACCCA 
GACAAAGATA AGAAAGGTCT AGAGAGCAGA GCAGACAGCA TCGGCAGCGG GCGAGCAATA 
CCAATTAAAC AGGGCATGCT ACTGAAAAGA AGCGGAAAGT CTCTTAACAA AGAGTGGAAG 
AAGAAATACG TAACCTTGTG TGATAACGGA GTTCTGACAT ATCATCCCAG CTTACACGAT 
TACATGCAAA ATGTCCATGG TAAAGAGATC GACCTCCTTA GAACTACTGT GAAGGTCCCT 
GGGAAAAGGC CTCCTCGAGC CACATCCTCT TGTGCACCAG TTGCCAGCCC TAAAACCAAT 
GGTCTAACCA AAGAGGTCAG CGGGCTCCAG ATTTCCCCTA ACACAGGGAA CGTGACCAGC 
AGTACGTCAG TCACCCAAAT GGCCAGTGGT CCAAGTGGGA TTAGTTTAGG CTCATTCAGC 
CGCATGGATG GGATGCACCA GAGGTCATAC TCTGTGTCTA GCGCTGACCA GTGGAGCGAG 
GGAGCCGTCA TCGCCAACTC AGCCATCAGC AGCGACACTG GCCTAGGAGA CTCAGTGTGT 
TCCAGTCCTA GCATCTCCAG CACTACAAGT CCCAAGCTAG ATCCGCCACC CTCGCCACAT 
GCAAACCGCA AGAAGCACCG CCGGAAGAAA AGTACCAGTA ACTTTAAGGC TGATGGGCTG 
TCAAGTACTG CTGAAGAACA AGAGGAGAAC TTTGAGTTTA TTATCGTATC CCTTACAAGT 
CAGAGCTGGC ACTTTGAAGC TACAAGCTAT GAGGAGAGGG ATGCATGGGT ACAGGCCATA 
GAGAGTCAGA TCTTGGCCAG CTTACAGTCC TGCGAAAGCA GCAAGAACAA GTCCCGGCTC 
ACCAGCCAGA ATGAAGCTTT AGCACTACAG TCGATCCGAA ATCTGCCAGG AAACTCGCAC 
TGTGTGGACT GTGATGCACA AAACCCAGAT TGGGCCAGTC TGAACCTGGG TGCCCTCATG 
TGCATTGAAT GTTCTGGGAT CCACCGTAAC CTTGGTACTC ACCTTTCCCG TGTCCGTTCC 
CTGGATCTGG ACGACTGGCC TCCAGAACTA ATTAAAGTGA TGTCAGCCAT CGGAAATGAG 
CTGGCCAACA GTGTGTGGGA AGGGAGCAGC CAAGGGCATG TAAAGCCATG TTCGGAGAGC 
CCAAGAGAAG AGAAGGAACG CTGGATACGT GCAAAGTATG AGCAAAAGCT TTTTCTGTCA 
CCGCTACCCT GCCGGGATTT ACCTCTGGGA CAGCAGCTTC TAAGGGCCAC AGCAGAGGAG 
GATTTGAGAG CTGTAATTTT GCTTCTTGCC CATGGTTCCA GGGAGGAGGT TAATGAGACC 
TGCGGGGAGG GAGACAGGCG CACATCCCTA CACCTGGCCT GTCGCAAGGG AAATGTAGTA 
CTCGTGCAAC TGCTTATATG GTACGGTGTG GATGTGATGG CAAGGGACTT CCATGGAAAC 
ACAGCATTGG CGTATGCCAA ACAAGCAGTG ACGTCTGAGG TGCGGGAGTT GCTTTTGCAA 
TATGGTTGCC CTGACGAACA ATTTGTGCTG ATGGCCACGC CAAACCTGTC TCGGAAAAAC 
AACCGCAACA ACAACAGCAA TGCAGGGGGC AGTGGCCTGA TGCCCACCTT AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917927.1
CDS	225..3761
Translation	MYLLDPAPPPMERGTPQRKTVYRISLTLVKKELLAESRLERCPEEEGEEQDTFSIHTGPRNFRTRSTGQLELGRLRVSRATSERKLGKIGGSPGDLSPGSREPWDSCAKDKVSPVVESPGSGPATERGPGVSHGVRSPQEVPKEEPCQNPLDYNNTKSPDKSIAPRTPLKSVISEDWGKAGPEGLCEKRHPSLLRRSFSFRHWNGELLRIRSLTREKHHSSSSCLTHHRADVREQMHVSATDPNAAEKRNTLDVGEVLNKSDPLTDLERWERSKSKNRTLDNSDLHKLSEQLELDRKGQERKLLRFFSGIFSRKDGSPASFAYPGGQSPRAAHSRSRGYFSSLKRPVTDNIQSSTESVNGSPYKDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRLANYRNTSEIPMVLVGTQDAISCSNPRVIDDSRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHTSVCSTQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPAASTPTPVRKQSKRRSNLFTSRKGSDPDKDKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSSCAPVASPKTNGLTKEVSGLQISPNTGNVTSSTSVTQMASGPSGISLGSFSRMDGMHQRSYSVSSADQWSEGAVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSSTAEEQEENFEFIIVSLTSQSWHFEATSYEERDAWVQAIESQILASLQSCESSKNKSRLTSQNEALALQSIRNLPGNSHCVDCDAQNPDWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPPELIKVMSAIGNELANSVWEGSSQGHVKPCSESPREEKERWIRAKYEQKLFLSPLPCRDLPLGQQLLRATAEEDLRAVILLLAHGSREEVNETCGEGDRRTSLHLACRKGNVVLVQLLIWYGVDVMARDFHGNTALAYAKQAVTSEVRELLLQYGCPDEQFVLMATPNLSRKNNRNNNSNAGGSGLMPTLI

Target ORF information:

RefSeq Version	XM_004917870.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (agap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917870.3

ORF Insert Sequence:

ATGTACCTGC TGGACCCCGC TCCGCCTCCA ATGGAACGAG GAACCCCCCA GAGAAAGACC 
GTGTACCGCA TCTCCCTTAC ACTGGTGAAG AAGGAGCTGC TGGCGGAGAG TCGGCTGGAG 
AGGTGTCCAG AGGAGGAGGG AGAGGAGCAG GATACCTTCT CCATACATAC TGGCCCCCGG 
AACTTCAGGA CCCGCAGTAC TGGGCAGCTG GAACTGGGCA GGCTGAGGGT GAGCAGGGCA 
ACCTCTGAAA GGAAGCTGGG TAAGATTGGG GGTTCACCTG GGGACCTGTC ACCTGGCTCT 
AGAGAGCCCT GGGATAGCTG TGCTAAGGAT AAAGTCTCCC CTGTGGTGGA GAGCCCAGGA 
AGTGGCCCTG CAACTGAAAG GGGTCCCGGG GTCAGCCATG GTGTTAGGAG CCCTCAAGAG 
GTTCCTAAGG AAGAGCCATG CCAAAATCCC TTGGATTACA ATAACACTAA GTCCCCAGAC 
AAGAGTATTG CTCCCAGGAC ACCTTTAAAA TCAGTAATAT CTGAGGACTG GGGCAAAGCT 
GGGCCAGAGG GGCTTTGTGA GAAGAGACAC CCCAGTCTCC TAAGGAGAAG CTTCAGCTTC 
CGCCACTGGA ATGGAGAGTT GCTGAGGATT AGGAGCCTGA CCAGAGAGAA GCACCACAGC 
AGCTCCAGCT GCCTCACTCA CCATAGGGCA GATGTCAGGG AGCAAATGCA CGTTTCTGCC 
ACTGACCCCA ACGCAGCGGA AAAGAGGAAT ACCCTCGATG TGGGGGAAGT GTTGAATAAA 
AGTGACCCCC TCACAGACCT CGAGAGATGG GAGAGGAGCA AGAGTAAGAA CAGGACCCTG 
GATAACAGTG ACCTCCACAA GCTTTCTGAG CAGCTGGAGT TGGACAGGAA AGGTCAGGAG 
AGGAAACTGC TCAGGTTCTT CAGTGGGATC TTCTCCAGGA AGGATGGCTC CCCAGCTTCA 
TTTGCTTACC CTGGGGGGCA GTCACCCAGG GCTGCTCATT CCAGATCTAG AGGATACTTT 
AGCAGCTTGA AAAGACCAGT AACAGATAAT ATTCAGTCCA GCACAGAGAG TGTTAATGGA 
TCTCCCTACA AAGATGCCTT TGTAAATAGT CAGGAATGGA CACTAAGCCG TTCTGTGCCA 
GAACTCAAAG TGGGCATTGT GGGGAACTTG GCCAGTGGCA AATCTGCTCT AGTGCATCGG 
TACCTGACTG GTACTTATGT ACAAGAGGAG TCCCCTGAAG ATATGGATGC AGGTGGGAGG 
TTCAAGAAGG AAATTGTAGT AGATGGACAG AGCTACCTTC TTCTGATCAG GGATGAAGGG 
GGACCCCCCG AAGCACAATT TGCCATGTGG GTGGATGCAG TTATCTTTGT CTTCAGCCTG 
GAAGATGAGA TCAGCTTTCA GACAGTCTAT CACTACTACA GCCGCCTTGC CAACTACCGC 
AACACCAGCG AGATCCCTAT GGTGCTGGTA GGCACTCAAG ATGCCATTAG CTGCAGTAAC 
CCCCGTGTAA TTGATGACAG CCGGGCACGA AAGCTTTCTA ATGACCTCAA GCGATGCACT 
TATTATGAGA CCTGCGCCAC CTATGGGCTA AATGTGGAGC GTGTTTTCCA AGATGTGGCC 
CAGAAGATTG TAGCCACCCG TAAGAAACAA CAGCTTTCCA TCGGACCCTG CAAGTCCCTG 
CCAAACTCCC CCAGCCACAC GTCTGTCTGT TCTACGCAGG TTTCGGCTGT TCACATTAGT 
CAGACCAGTA ATGGTGGGGG TAGCCTAAGC GACTATTCCT CATCTGTCCC CTCTACACCG 
AGTACTAGTC AGAAGGAGCT GAGGATCGAC GTGCCCCCAG CAGCCAGCAC ACCCACTCCC 
GTGCGCAAGC AATCAAAACG GCGCTCCAAT CTCTTCACGT CTCGCAAAGG GAGCGACCCA 
GACAAAGATA AGAAAGGTCT AGAGAGCAGA GCAGACAGCA TCGGCAGCGG GCGAGCAATA 
CCAATTAAAC AGGGCATGCT ACTGAAAAGA AGCGGAAAGT CTCTTAACAA AGAGTGGAAG 
AAGAAATACG TAACCTTGTG TGATAACGGA GTTCTGACAT ATCATCCCAG CTTACACGAT 
TACATGCAAA ATGTCCATGG TAAAGAGATC GACCTCCTTA GAACTACTGT GAAGGTCCCT 
GGGAAAAGGC CTCCTCGAGC CACATCCTCT TGTGCACCAG TTGCCAGCCC TAAAACCAAT 
GGTCTAACCA AAGAGGTCAG CGGGCTCCAG ATTTCCCCTA ACACAGGGAA CGTGACCAGC 
AGTACGTCAG TCACCCAAAT GGCCAGTGGT CCAAGTGGGA TTAGTTTAGG CTCATTCAGC 
CGCATGGATG GGATGCACCA GAGGTCATAC TCTGTGTCTA GCGCTGACCA GTGGAGCGAG 
GGAGCCGTCA TCGCCAACTC AGCCATCAGC AGCGACACTG GCCTAGGAGA CTCAGTGTGT 
TCCAGTCCTA GCATCTCCAG CACTACAAGT CCCAAGCTAG ATCCGCCACC CTCGCCACAT 
GCAAACCGCA AGAAGCACCG CCGGAAGAAA AGTACCAGTA ACTTTAAGGC TGATGGGCTG 
TCAAGTACTG CTGAAGAACA AGAGGAGAAC TTTGAGTTTA TTATCGTATC CCTTACAAGT 
CAGAGCTGGC ACTTTGAAGC TACAAGCTAT GAGGAGAGGG ATGCATGGGT ACAGGCCATA 
GAGAGTCAGA TCTTGGCCAG CTTACAGTCC TGCGAAAGCA GCAAGAACAA GTCCCGGCTC 
ACCAGCCAGA ATGAAGCTTT AGCACTACAG TCGATCCGAA ATCTGCCAGG AAACTCGCAC 
TGTGTGGACT GTGATGCACA AAACCCAGAT TGGGCCAGTC TGAACCTGGG TGCCCTCATG 
TGCATTGAAT GTTCTGGGAT CCACCGTAAC CTTGGTACTC ACCTTTCCCG TGTCCGTTCC 
CTGGATCTGG ACGACTGGCC TCCAGAACTA ATTAAAGTGA TGTCAGCCAT CGGAAATGAG 
CTGGCCAACA GTGTGTGGGA AGGGAGCAGC CAAGGGCATG TAAAGCCATG TTCGGAGAGC 
CCAAGAGAAG AGAAGGAACG CTGGATACGT GCAAAGTATG AGCAAAAGCT TTTTCTGTCA 
CCGCTACCCT GCCGGGATTT ACCTCTGGGA CAGCAGCTTC TAAGGGCCAC AGCAGAGGAG 
GATTTGAGAG CTGTAATTTT GCTTCTTGCC CATGGTTCCA GGGAGGAGGT TAATGAGACC 
TGCGGGGAGG GAGACAGGCG CACATCCCTA CACCTGGCCT GTCGCAAGGG AAATGTAGTA 
CTCGTGCAAC TGCTTATATG GTACGGTGTG GATGTGATGG CAAGGGACTT CCATGGAAAC 
ACAGCATTGG CGTATGCCAA ACAAGCAGTG ACGTCTGAGG TGCGGGAGTT GCTTTTGCAA 
TATGGTTGCC CTGACGAACA ATTTGTGCTG ATGGCCACGC CAAACCTGTC TCGGAAAAAC 
AACCGCAACA ACAACAGCAA TGCAGGGGGC AGTGGCCTGA TGCCCACCTT AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	agap1
Entrez Gene ID	100485248
Full Name	ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms	centg2,ggap1
General protein information	Preferred Names ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 Names arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Mus musculus (house mouse)	Agap1	NP_835220.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009160	XP_319921.4
Xenopus tropicalis (tropical clawed frog)	agap1	XP_002932044.1
Caenorhabditis elegans (roundworm)	cnt-2	NP_001022837.1
Gallus gallus (chicken)	AGAP1	XP_004942549.1
Drosophila melanogaster (fruit fly)	cenG1A	NP_723849.1
Canis lupus familiaris (dog)	AGAP1	XP_005635967.1
Homo sapiens (human)	AGAP1	NP_001032208.1
	AGAP1	XP_001082607.1
Rattus norvegicus (Norway rat)	Agap1	NP_001101700.1
Danio rerio (zebrafish)	agap1	XP_001921526.3

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agap1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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