tet2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tet2
Entrez Gene ID	100380145
Full Name	tet methylcytosine dioxygenase 2
General protein information	Preferred Names tet methylcytosine dioxygenase 2 Names methylcytosine dioxygenase TET2 tet oncogene family member 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934777.5	XP_002934823.2	methylcytosine dioxygenase TET2 isoform X1
XM_012955515.3	XP_012810969.1	methylcytosine dioxygenase TET2 isoform X2
XM_012955515.2	XP_012810969.1	methylcytosine dioxygenase TET2 isoform X2
XM_002934777.4	XP_002934823.2	methylcytosine dioxygenase TET2 isoform X1
XM_018096256.1	XP_017951745.1	methylcytosine dioxygenase TET2 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TET2	NP_001120680.1
Canis lupus familiaris (dog)	TET2	XP_535678.3
Rattus norvegicus (Norway rat)	Tet2	XP_006233409.1
Gallus gallus (chicken)	TET2	NP_001264723.1
Bos taurus (cattle)	TET2	XP_005207739.1
Pan troglodytes (chimpanzee)	TET2	XP_526645.2
	TET2	XP_001082840.2
Mus musculus (house mouse)	Tet2	NP_001035490.2
Xenopus tropicalis (tropical clawed frog)	tet2	XP_002934823.2

Gene Ontology

The following tet2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tet2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa48202	XM_002934777.5 Latest version!	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa48203	XM_012955515.3 Latest version!	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa04496	XM_002934777.4 Latest version!	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa33117	XM_018096256.1 Latest version!	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa33117	XM_012955515.2 Latest version!	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tet2 ( XM_002934777.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934823.2 Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X1, mRNA.

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CloneID	OXa48202
Clone ID Related Accession (Same CDS sequence)	XM_002934777.5
Accession Version	XM_002934777.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	methylcytosine dioxygenase TET2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934777.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCCA AAGCTAACCT ATATGTGGAT TGCTACTTTG TGCTTCTGCA GATACGTTTA 
CAGGTCAGTC TTGTGGATGG CCCGGGAGCA GGCCAGATGG AAGAGGACAG AACCAACCAT 
GACGAGGGCA ATAGATTGAG TCCAACCCTA TTATATTCTT CGTCAGCTTG CCAAAACAAG 
AACGGTCTAT TACAAAATGG GAACCTGCCA ACAGACAAAG ACCTTCTAGT TAATGGGGAC 
ATATGTTTAC CAACCAAAAA CAACTATAAT GTTGCTTACA TGAAGGGAAG TCAAGAACAT 
GGTGCTTCTG CTCTCATCCA TGACAACAAA GCTTATCCCA ACCACATACA AAATGGTGGA 
ATAAAGCGCA CATATAGTGA ACCGTTGTTT TCTGAATCCC AGTTGAAAAA GGTCAGAACA 
GATGAGATTA ATGGAGAAAA TAAGGGTGAC CAAAGGCAAA ATAATATTCA AGCAAGTGTC 
TCTGAGTTCT GCAATGAAGA TCAGTCAGTA AGAATTTTCC AACCAGTTAA TTCACCAGCT 
CTTCAGGTTC CAAATGAGCA GAAGAAAGAC CTTAATTACA GCAACAGAGA CATTGTCTCA 
TGTCTTAAAG ACAAGACAGT GCCAATGTGT AATGGTGCTA CTGCTTCTGT ATCTTCCATG 
GAAAACGTGA ATAATGAGCT CCTGGAAAAA CTGCCTTCTC AATATTATCC TGAACATGTT 
TCTACTGTGG TGCATAATAA CACATCTCTT GCAAATGCCA TTAATACGCT AAATGATGCT 
AGCAATGATT TGTCTACTCT GGCAACTCAT CCGTCACATA CCTCAGGGCA GATGACTCCT 
CAACAAACTT CGGTGCTGCC TCAAGTGTCA GGTGCAATTG TTGATGGGGC CCCCAATAGT 
GACCATGGTA GTGAGCCACT TGTATTACCA GGAAGCTATT CACTTAATAG ACAAGAACAG 
AAGCCACTTT CACAGGAAAC TCTTAATTAT AATTCCCATA ATTTAGTTGG AAGCATGGGT 
GATGCATGCA AATCCATGGG CCAGATGTCA GCACAATGTC TAGATGTTTC TGGTAATGGT 
TCATTTCATA TCCAAAATAG CTCATCAAAT GAGATAGCTT CTGAATTAGA GGCCATCAAG 
AAAGATCCAG CACCATCACT GCAATTACAT CATATTATGC CCAACAAGCA AAATGAGTCC 
AGTATAAGTC AATCACATGA TGGGCATCCA ATTGCATCAC AAACTAATGA ATCTTGCCAT 
TCACATGATC CAAGAGTATC TCCACATCCT AATGATCAAC AGTTACAAAA TGAAAGAACG 
GCCATGTTTC CTCTCACCAG ATTACCTGCA CTACACAGTT CGGTTAATGA AATTCTAGCA 
AAACACAAGT TTCACAACAG TTTAGAGATG CAAAATGAGC ATCTCAGGCA AATCAAAGTC 
ATGCAGAATT GCAATGGGAC AAGTAAAGAT GATTCTTTCA TTAAGTCTAC AAAAGAATTT 
CAATCCAATC CTTTTTTAAA ATCAGGCTGG TTAGAACTTC GCCCTAATCA GCGCCTAAAA 
GAAGGATCTT CTGAAACTTC TTGTGAAAAT TTACTGAACT TACTTCTCCA GTCCCAGACT 
AATAACAAGA AAAAGCTAAG ACAATACCCT GGAAAACAAG GTTTAGCAAA TAGAAAGTGT 
AACAGTCAAG ATAGTGAAGA ACATCTGCAG CCAAATATTT CTGAGCAGCA GCCAAATTTC 
CCAAAGCACT TATCACAAGA TTCTCAATAC CAGCCATACA GTCAAAAAAC TAATAATGGT 
CTGATGTGGC CTGATCTTGA ACAAGCTTCT GAGGGCACTC AGTTAGGTGA TGTCCAACAT 
TTTTTGCAAG CACAGCCAAG TGAATATATT GAAGAGCTGT TAAAACAACA TTTTGATCAG 
TCACTCCGTA ACTGTAACCA AAATAGGGAC AGTCCTCTTA ACCACTTTAA ACTTGAAGAA 
GGTGCTCAGG CAGCTAATCA GTATCAAAAA CACCATCCAT ATCCTCAGCA ATTTGAGATG 
GAACCTAAGC AAAGTTTAAG CAACATGAAT TCCCCCTTCA GTCAAATTCA AGAAAATAAT 
ACAGTTAACC CTAACCATTC CTATCCTGAC AAACAAATAT ATCTGTCACA AAAAACAGAA 
AATAGCTGTG GTTTTGCAGA GAACAAACCT GACCCTTTGC AATACATGCA GTATTATCAA 
GCAAATAGTA CTGAGCAACA AGAAATAAGC AATTATGAGC ATGATCTTTC GGCCCGAATA 
GCAAAGCAGA CACATTGCCT GCCAGAGAGC TTTAGTTCAT CCCCACATTT GCATGATAAA 
ATGGGATATT ATTCTCAATT TGAAAACCAG GTTGACTGTA TTCCAAAGCA TGCTGCTCTT 
AGGAATCACT TGCTGCAACG AAGTAGATCA GTTAAAGCTG ATAGATTGGT TAAAGCTGAG 
AAGAGTTCCC ACTCTAATGC CTGCATGCAT ACACCTATGG AAAGCAAAAC TAAAAAAAAC 
ACAGATAAAA AAATCAAACA AGAAACTCGC CATTCAAGCA CAGAGGCTGG ACAACAAAAG 
AGTATTATTG AAACAATGGA ACAGCAGATG AGACATTATC CTGGTAAATC ATCGTTTCAT 
CGTCAAGTAT CAAGTATCAA ATCTCCCAAG CATGTGAAGG TGGAGTCTTC AGGGCCTGTC 
ACAGTCCTAA CTACACACAC TAATTTTTCT ACACTAGACC AACAAACTGT TCCTCCGCAT 
GAGAAAACAC CAACCAAAAG AGCAGCAGGT TCTGCTCTAC ATAACTTTAT AGAATCACCC 
TCAAATCTAC TCAGTACACC AGTAAAAAAT CTTTTGGACA CACCTATAAA AACGCAGTAT 
GATTTTCCAT CATGCAGTTG TGTAGATCAA ATAATTGAAA AAGATGAAGG ACCTTATTAT 
ACACATCTTG GTGCTGGACC CAACGTGGCA GCTATCAGAG AAATGATGGA AGAAAGGTTT 
GGCCAAAAGG GGAATGCCAT CAGAATTGAG AGGGTTGTTT ATACAGGAAA AGAAGGCAAA 
AGTGCTCAGG GATGTCCTAT TGCTAAATGG GTGATTCGGC GTAGTGGGAC AGATGAGAAA 
ATGCTGTGTT TGGTGAGGGA ACGTGCTGGT CACTCCTGCG AAACAGCAGT GATTGTCATT 
CTGATCTTGG TTTGGGAAGG CATTTCATTC TCTTTGGCAG ACAGGCTTTA CTCAGAGCTC 
ACAGAAACGC TAAATAAGTA TGGAACCTTA ACAAACCGAC GCTGTGCACG TAACGAAGAA 
CGTACCTGTG CATGCCAGGG TCTGGACTCT GAAACCTGCG GTGCCTCCTT TTCCTTTGGG 
TGCTCATGGA GTATGTATTA TAACGGGTGT AAATTCGCTA GAAGTAAAGT ACCAAGAAAA 
TTCAAACTCC TTGGTGATGA TCCAAAATCG GAAGAAAAGT TGGAAGCTAA TTTACAGCAC 
TTATCAACAC TGATGGCTCC AATTTACAAG AAGCTTGCCC CTGATGCCTA TCATAACCAG 
ATTGAGCATG AGCACAGAGC TCCAGATTGC CGTCTTGGTT TGAAGGAAGG TCGACCATTT 
TCTGGGGTTA CTGCTTGCTT GGATTTTTGC GCTCATTCCC ACCGGGACTT GCACAACATG 
CAAAATGGCA GCACACTGGT TTGCACTTTA ACCAGAGAAG ACAATCGTGA AAATGGAAAA 
ATTCCACAAG ATGAGCAACT GCATGTCTTA CCCCTTTATA AAGTATCCAA TGTGGATGAG 
TTTGGCAGTT CTGAGAGTCA GGAAGAGAAG AAAAGAACTG GTGCTATTCA AGTTCTCACA 
TCTTTCCGAA GGCAAGTGCG AATGCTAGCT GAGCCTGTAA AAACTTGCCG GCAAAAGAAG 
TTGGAAAAAC AAGAAGCCAA GAAAGCAGCA GCAGAGAAGT TAAATGCCGC TTTAGAAAAT 
GGTCACAGCA AGAACGAAAA GGAGAAGTCC TCACGGCATA AAAACGCACA TGTAGAAGCT 
TCTACGCAGG ACAAACAGCA AGCAGAATTT TCTCGAATGC CAGCCTTGCA ACAAAACACC 
CTTCTCAATA TGAACCATAG GAACTCTTTA AACCATTATC CATCATCCGC CCCTACAAAT 
CCTTACACAC GAATTCCTAA TCTGCCTGAC CCATATCAGA ATAGTTTTTC TCCCTACCCT 
GGATCAAGCC ACATGAATAT TTTTCAAACC GCTGCAAGCT CATCAGTATC CTGTGCAAAT 
AGCCCTATGA ATTCTTTTCA AGGATCCTTT AATCAAGGTA ACTCTTGCCC CTCTTATCCC 
AGTAATGGAA ATGCCTCCTT GCACAACTGT CCCCCTTATC TTGACTCTTC ACAGCAACAA 
TCCATGGATT ATTACAAGTT TAAAAACCAA GATTCAATGG CTAAATTAGG TGTGCCCCCA 
ATTCAATCAT TGTACCAGCA GGGGTTTGCT AATAATCAGG CTTATGGTAA CAAAATGTTT 
AGTTATGGAA ACCAAAGAGT GCAAGCTGAT GATTTCAGTG GCTGTAATGC TTATCCAGCA 
CCTCAGGCTG ATATAATGAA TCCAGCCACA AGTTCACGGC CTATTACCAG TAATCAAGAC 
TTCTCTTCTG CGACTAAGAA TGGTGAATAT CCTCTTCCAC ACTCCTTCCA GGGTCATGAA 
AGCAACATGT CATCAAGTAT ATTGGGAGGC AGAGCTGGTT CTCTGCCATT CCCTGGTAAA 
CAGAGTGAAA TGCATTCACG CACAGCAAAT GGAACATCGG AAATGCTTCC AGGTCTTAAC 
CATGATAGAA CTAATTTTGG AGGCGCAGGC AGAACTAATG GTGCTAATGT TCAGGGAAAG 
CTACCGTACA CCTTACCTCC TGTACCAGAA GAGCCTGCAG AAGTTTGGTC AGACAGTGAA 
CAGAATTTTC TTGATCCTGA TATAGGAGGT GTAGCAGTTT GCCCAACTCA TGGGTCAATC 
TTGATTGAAT GTGCAAAGCG TGAGCTCCAC GCAACAACAC CTTTAAAGAA TCCCAACAGA 
AATCACCCTA CCAGAATATC TTTGGTGTTC TACCAACACA AGAGCATGAA CGAGCCAAAG 
CATGGCCTGG CATTGTGGGA AGCAAAAATG GCAGGCAAAG CCAGGGAAAA GGAAGAAGAT 
TGTGAAAGGT ATGGTCCAGA TTATGTGGCT CCTAAATCTT ACAACAGGAA AGCAAAGCGG 
GAGCCTGTGG AGCCAATACA TGAACCTGCT GAACCAACTT ATCTTCGTTT CATCAAGTCA 
CTTACGCAGA GGACGATGTC AATAACCACA GACTCTCAAG TCACTACATC TCCATATGCC 
TTAACACGGG TTACAGGTCC TTATAACAGA TATATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934823.2
CDS	140..5518
Translation	MTPKANLYVDCYFVLLQIRLQVSLVDGPGAGQMEEDRTNHDEGNRLSPTLLYSSSACQNKNGLLQNGNLPTDKDLLVNGDICLPTKNNYNVAYMKGSQEHGASALIHDNKAYPNHIQNGGIKRTYSEPLFSESQLKKVRTDEINGENKGDQRQNNIQASVSEFCNEDQSVRIFQPVNSPALQVPNEQKKDLNYSNRDIVSCLKDKTVPMCNGATASVSSMENVNNELLEKLPSQYYPEHVSTVVHNNTSLANAINTLNDASNDLSTLATHPSHTSGQMTPQQTSVLPQVSGAIVDGAPNSDHGSEPLVLPGSYSLNRQEQKPLSQETLNYNSHNLVGSMGDACKSMGQMSAQCLDVSGNGSFHIQNSSSNEIASELEAIKKDPAPSLQLHHIMPNKQNESSISQSHDGHPIASQTNESCHSHDPRVSPHPNDQQLQNERTAMFPLTRLPALHSSVNEILAKHKFHNSLEMQNEHLRQIKVMQNCNGTSKDDSFIKSTKEFQSNPFLKSGWLELRPNQRLKEGSSETSCENLLNLLLQSQTNNKKKLRQYPGKQGLANRKCNSQDSEEHLQPNISEQQPNFPKHLSQDSQYQPYSQKTNNGLMWPDLEQASEGTQLGDVQHFLQAQPSEYIEELLKQHFDQSLRNCNQNRDSPLNHFKLEEGAQAANQYQKHHPYPQQFEMEPKQSLSNMNSPFSQIQENNTVNPNHSYPDKQIYLSQKTENSCGFAENKPDPLQYMQYYQANSTEQQEISNYEHDLSARIAKQTHCLPESFSSSPHLHDKMGYYSQFENQVDCIPKHAALRNHLLQRSRSVKADRLVKAEKSSHSNACMHTPMESKTKKNTDKKIKQETRHSSTEAGQQKSIIETMEQQMRHYPGKSSFHRQVSSIKSPKHVKVESSGPVTVLTTHTNFSTLDQQTVPPHEKTPTKRAAGSALHNFIESPSNLLSTPVKNLLDTPIKTQYDFPSCSCVDQIIEKDEGPYYTHLGAGPNVAAIREMMEERFGQKGNAIRIERVVYTGKEGKSAQGCPIAKWVIRRSGTDEKMLCLVRERAGHSCETAVIVILILVWEGISFSLADRLYSELTETLNKYGTLTNRRCARNEERTCACQGLDSETCGASFSFGCSWSMYYNGCKFARSKVPRKFKLLGDDPKSEEKLEANLQHLSTLMAPIYKKLAPDAYHNQIEHEHRAPDCRLGLKEGRPFSGVTACLDFCAHSHRDLHNMQNGSTLVCTLTREDNRENGKIPQDEQLHVLPLYKVSNVDEFGSSESQEEKKRTGAIQVLTSFRRQVRMLAEPVKTCRQKKLEKQEAKKAAAEKLNAALENGHSKNEKEKSSRHKNAHVEASTQDKQQAEFSRMPALQQNTLLNMNHRNSLNHYPSSAPTNPYTRIPNLPDPYQNSFSPYPGSSHMNIFQTAASSSVSCANSPMNSFQGSFNQGNSCPSYPSNGNASLHNCPPYLDSSQQQSMDYYKFKNQDSMAKLGVPPIQSLYQQGFANNQAYGNKMFSYGNQRVQADDFSGCNAYPAPQADIMNPATSSRPITSNQDFSSATKNGEYPLPHSFQGHESNMSSSILGGRAGSLPFPGKQSEMHSRTANGTSEMLPGLNHDRTNFGGAGRTNGANVQGKLPYTLPPVPEEPAEVWSDSEQNFLDPDIGGVAVCPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAGKAREKEEDCERYGPDYVAPKSYNRKAKREPVEPIHEPAEPTYLRFIKSLTQRTMSITTDSQVTTSPYALTRVTGPYNRYI

Target ORF information:

RefSeq Version	XM_002934777.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934777.5

ORF Insert Sequence:

ATGACCCCCA AAGCTAACCT ATATGTGGAT TGCTACTTTG TGCTTCTGCA GATACGTTTA 
CAGGTCAGTC TTGTGGATGG CCCGGGAGCA GGCCAGATGG AAGAGGACAG AACCAACCAT 
GACGAGGGCA ATAGATTGAG TCCAACCCTA TTATATTCTT CGTCAGCTTG CCAAAACAAG 
AACGGTCTAT TACAAAATGG GAACCTGCCA ACAGACAAAG ACCTTCTAGT TAATGGGGAC 
ATATGTTTAC CAACCAAAAA CAACTATAAT GTTGCTTACA TGAAGGGAAG TCAAGAACAT 
GGTGCTTCTG CTCTCATCCA TGACAACAAA GCTTATCCCA ACCACATACA AAATGGTGGA 
ATAAAGCGCA CATATAGTGA ACCGTTGTTT TCTGAATCCC AGTTGAAAAA GGTCAGAACA 
GATGAGATTA ATGGAGAAAA TAAGGGTGAC CAAAGGCAAA ATAATATTCA AGCAAGTGTC 
TCTGAGTTCT GCAATGAAGA TCAGTCAGTA AGAATTTTCC AACCAGTTAA TTCACCAGCT 
CTTCAGGTTC CAAATGAGCA GAAGAAAGAC CTTAATTACA GCAACAGAGA CATTGTCTCA 
TGTCTTAAAG ACAAGACAGT GCCAATGTGT AATGGTGCTA CTGCTTCTGT ATCTTCCATG 
GAAAACGTGA ATAATGAGCT CCTGGAAAAA CTGCCTTCTC AATATTATCC TGAACATGTT 
TCTACTGTGG TGCATAATAA CACATCTCTT GCAAATGCCA TTAATACGCT AAATGATGCT 
AGCAATGATT TGTCTACTCT GGCAACTCAT CCGTCACATA CCTCAGGGCA GATGACTCCT 
CAACAAACTT CGGTGCTGCC TCAAGTGTCA GGTGCAATTG TTGATGGGGC CCCCAATAGT 
GACCATGGTA GTGAGCCACT TGTATTACCA GGAAGCTATT CACTTAATAG ACAAGAACAG 
AAGCCACTTT CACAGGAAAC TCTTAATTAT AATTCCCATA ATTTAGTTGG AAGCATGGGT 
GATGCATGCA AATCCATGGG CCAGATGTCA GCACAATGTC TAGATGTTTC TGGTAATGGT 
TCATTTCATA TCCAAAATAG CTCATCAAAT GAGATAGCTT CTGAATTAGA GGCCATCAAG 
AAAGATCCAG CACCATCACT GCAATTACAT CATATTATGC CCAACAAGCA AAATGAGTCC 
AGTATAAGTC AATCACATGA TGGGCATCCA ATTGCATCAC AAACTAATGA ATCTTGCCAT 
TCACATGATC CAAGAGTATC TCCACATCCT AATGATCAAC AGTTACAAAA TGAAAGAACG 
GCCATGTTTC CTCTCACCAG ATTACCTGCA CTACACAGTT CGGTTAATGA AATTCTAGCA 
AAACACAAGT TTCACAACAG TTTAGAGATG CAAAATGAGC ATCTCAGGCA AATCAAAGTC 
ATGCAGAATT GCAATGGGAC AAGTAAAGAT GATTCTTTCA TTAAGTCTAC AAAAGAATTT 
CAATCCAATC CTTTTTTAAA ATCAGGCTGG TTAGAACTTC GCCCTAATCA GCGCCTAAAA 
GAAGGATCTT CTGAAACTTC TTGTGAAAAT TTACTGAACT TACTTCTCCA GTCCCAGACT 
AATAACAAGA AAAAGCTAAG ACAATACCCT GGAAAACAAG GTTTAGCAAA TAGAAAGTGT 
AACAGTCAAG ATAGTGAAGA ACATCTGCAG CCAAATATTT CTGAGCAGCA GCCAAATTTC 
CCAAAGCACT TATCACAAGA TTCTCAATAC CAGCCATACA GTCAAAAAAC TAATAATGGT 
CTGATGTGGC CTGATCTTGA ACAAGCTTCT GAGGGCACTC AGTTAGGTGA TGTCCAACAT 
TTTTTGCAAG CACAGCCAAG TGAATATATT GAAGAGCTGT TAAAACAACA TTTTGATCAG 
TCACTCCGTA ACTGTAACCA AAATAGGGAC AGTCCTCTTA ACCACTTTAA ACTTGAAGAA 
GGTGCTCAGG CAGCTAATCA GTATCAAAAA CACCATCCAT ATCCTCAGCA ATTTGAGATG 
GAACCTAAGC AAAGTTTAAG CAACATGAAT TCCCCCTTCA GTCAAATTCA AGAAAATAAT 
ACAGTTAACC CTAACCATTC CTATCCTGAC AAACAAATAT ATCTGTCACA AAAAACAGAA 
AATAGCTGTG GTTTTGCAGA GAACAAACCT GACCCTTTGC AATACATGCA GTATTATCAA 
GCAAATAGTA CTGAGCAACA AGAAATAAGC AATTATGAGC ATGATCTTTC GGCCCGAATA 
GCAAAGCAGA CACATTGCCT GCCAGAGAGC TTTAGTTCAT CCCCACATTT GCATGATAAA 
ATGGGATATT ATTCTCAATT TGAAAACCAG GTTGACTGTA TTCCAAAGCA TGCTGCTCTT 
AGGAATCACT TGCTGCAACG AAGTAGATCA GTTAAAGCTG ATAGATTGGT TAAAGCTGAG 
AAGAGTTCCC ACTCTAATGC CTGCATGCAT ACACCTATGG AAAGCAAAAC TAAAAAAAAC 
ACAGATAAAA AAATCAAACA AGAAACTCGC CATTCAAGCA CAGAGGCTGG ACAACAAAAG 
AGTATTATTG AAACAATGGA ACAGCAGATG AGACATTATC CTGGTAAATC ATCGTTTCAT 
CGTCAAGTAT CAAGTATCAA ATCTCCCAAG CATGTGAAGG TGGAGTCTTC AGGGCCTGTC 
ACAGTCCTAA CTACACACAC TAATTTTTCT ACACTAGACC AACAAACTGT TCCTCCGCAT 
GAGAAAACAC CAACCAAAAG AGCAGCAGGT TCTGCTCTAC ATAACTTTAT AGAATCACCC 
TCAAATCTAC TCAGTACACC AGTAAAAAAT CTTTTGGACA CACCTATAAA AACGCAGTAT 
GATTTTCCAT CATGCAGTTG TGTAGATCAA ATAATTGAAA AAGATGAAGG ACCTTATTAT 
ACACATCTTG GTGCTGGACC CAACGTGGCA GCTATCAGAG AAATGATGGA AGAAAGGTTT 
GGCCAAAAGG GGAATGCCAT CAGAATTGAG AGGGTTGTTT ATACAGGAAA AGAAGGCAAA 
AGTGCTCAGG GATGTCCTAT TGCTAAATGG GTGATTCGGC GTAGTGGGAC AGATGAGAAA 
ATGCTGTGTT TGGTGAGGGA ACGTGCTGGT CACTCCTGCG AAACAGCAGT GATTGTCATT 
CTGATCTTGG TTTGGGAAGG CATTTCATTC TCTTTGGCAG ACAGGCTTTA CTCAGAGCTC 
ACAGAAACGC TAAATAAGTA TGGAACCTTA ACAAACCGAC GCTGTGCACG TAACGAAGAA 
CGTACCTGTG CATGCCAGGG TCTGGACTCT GAAACCTGCG GTGCCTCCTT TTCCTTTGGG 
TGCTCATGGA GTATGTATTA TAACGGGTGT AAATTCGCTA GAAGTAAAGT ACCAAGAAAA 
TTCAAACTCC TTGGTGATGA TCCAAAATCG GAAGAAAAGT TGGAAGCTAA TTTACAGCAC 
TTATCAACAC TGATGGCTCC AATTTACAAG AAGCTTGCCC CTGATGCCTA TCATAACCAG 
ATTGAGCATG AGCACAGAGC TCCAGATTGC CGTCTTGGTT TGAAGGAAGG TCGACCATTT 
TCTGGGGTTA CTGCTTGCTT GGATTTTTGC GCTCATTCCC ACCGGGACTT GCACAACATG 
CAAAATGGCA GCACACTGGT TTGCACTTTA ACCAGAGAAG ACAATCGTGA AAATGGAAAA 
ATTCCACAAG ATGAGCAACT GCATGTCTTA CCCCTTTATA AAGTATCCAA TGTGGATGAG 
TTTGGCAGTT CTGAGAGTCA GGAAGAGAAG AAAAGAACTG GTGCTATTCA AGTTCTCACA 
TCTTTCCGAA GGCAAGTGCG AATGCTAGCT GAGCCTGTAA AAACTTGCCG GCAAAAGAAG 
TTGGAAAAAC AAGAAGCCAA GAAAGCAGCA GCAGAGAAGT TAAATGCCGC TTTAGAAAAT 
GGTCACAGCA AGAACGAAAA GGAGAAGTCC TCACGGCATA AAAACGCACA TGTAGAAGCT 
TCTACGCAGG ACAAACAGCA AGCAGAATTT TCTCGAATGC CAGCCTTGCA ACAAAACACC 
CTTCTCAATA TGAACCATAG GAACTCTTTA AACCATTATC CATCATCCGC CCCTACAAAT 
CCTTACACAC GAATTCCTAA TCTGCCTGAC CCATATCAGA ATAGTTTTTC TCCCTACCCT 
GGATCAAGCC ACATGAATAT TTTTCAAACC GCTGCAAGCT CATCAGTATC CTGTGCAAAT 
AGCCCTATGA ATTCTTTTCA AGGATCCTTT AATCAAGGTA ACTCTTGCCC CTCTTATCCC 
AGTAATGGAA ATGCCTCCTT GCACAACTGT CCCCCTTATC TTGACTCTTC ACAGCAACAA 
TCCATGGATT ATTACAAGTT TAAAAACCAA GATTCAATGG CTAAATTAGG TGTGCCCCCA 
ATTCAATCAT TGTACCAGCA GGGGTTTGCT AATAATCAGG CTTATGGTAA CAAAATGTTT 
AGTTATGGAA ACCAAAGAGT GCAAGCTGAT GATTTCAGTG GCTGTAATGC TTATCCAGCA 
CCTCAGGCTG ATATAATGAA TCCAGCCACA AGTTCACGGC CTATTACCAG TAATCAAGAC 
TTCTCTTCTG CGACTAAGAA TGGTGAATAT CCTCTTCCAC ACTCCTTCCA GGGTCATGAA 
AGCAACATGT CATCAAGTAT ATTGGGAGGC AGAGCTGGTT CTCTGCCATT CCCTGGTAAA 
CAGAGTGAAA TGCATTCACG CACAGCAAAT GGAACATCGG AAATGCTTCC AGGTCTTAAC 
CATGATAGAA CTAATTTTGG AGGCGCAGGC AGAACTAATG GTGCTAATGT TCAGGGAAAG 
CTACCGTACA CCTTACCTCC TGTACCAGAA GAGCCTGCAG AAGTTTGGTC AGACAGTGAA 
CAGAATTTTC TTGATCCTGA TATAGGAGGT GTAGCAGTTT GCCCAACTCA TGGGTCAATC 
TTGATTGAAT GTGCAAAGCG TGAGCTCCAC GCAACAACAC CTTTAAAGAA TCCCAACAGA 
AATCACCCTA CCAGAATATC TTTGGTGTTC TACCAACACA AGAGCATGAA CGAGCCAAAG 
CATGGCCTGG CATTGTGGGA AGCAAAAATG GCAGGCAAAG CCAGGGAAAA GGAAGAAGAT 
TGTGAAAGGT ATGGTCCAGA TTATGTGGCT CCTAAATCTT ACAACAGGAA AGCAAAGCGG 
GAGCCTGTGG AGCCAATACA TGAACCTGCT GAACCAACTT ATCTTCGTTT CATCAAGTCA 
CTTACGCAGA GGACGATGTC AATAACCACA GACTCTCAAG TCACTACATC TCCATATGCC 
TTAACACGGG TTACAGGTCC TTATAACAGA TATATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet2 ( XM_012955515.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810969.1 Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X2, mRNA.

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CloneID	OXa48203
Clone ID Related Accession (Same CDS sequence)	XM_012955515.3
Accession Version	XM_012955515.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	methylcytosine dioxygenase TET2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955515.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG ACAGAACCAA CCATGACGAG GGCAATAGAT TGAGTCCAAC CCTATTATAT 
TCTTCGTCAG CTTGCCAAAA CAAGAACGGT CTATTACAAA ATGGGAACCT GCCAACAGAC 
AAAGACCTTC TAGTTAATGG GGACATATGT TTACCAACCA AAAACAACTA TAATGTTGCT 
TACATGAAGG GAAGTCAAGA ACATGGTGCT TCTGCTCTCA TCCATGACAA CAAAGCTTAT 
CCCAACCACA TACAAAATGG TGGAATAAAG CGCACATATA GTGAACCGTT GTTTTCTGAA 
TCCCAGTTGA AAAAGGTCAG AACAGATGAG ATTAATGGAG AAAATAAGGG TGACCAAAGG 
CAAAATAATA TTCAAGCAAG TGTCTCTGAG TTCTGCAATG AAGATCAGTC AGTAAGAATT 
TTCCAACCAG TTAATTCACC AGCTCTTCAG GTTCCAAATG AGCAGAAGAA AGACCTTAAT 
TACAGCAACA GAGACATTGT CTCATGTCTT AAAGACAAGA CAGTGCCAAT GTGTAATGGT 
GCTACTGCTT CTGTATCTTC CATGGAAAAC GTGAATAATG AGCTCCTGGA AAAACTGCCT 
TCTCAATATT ATCCTGAACA TGTTTCTACT GTGGTGCATA ATAACACATC TCTTGCAAAT 
GCCATTAATA CGCTAAATGA TGCTAGCAAT GATTTGTCTA CTCTGGCAAC TCATCCGTCA 
CATACCTCAG GGCAGATGAC TCCTCAACAA ACTTCGGTGC TGCCTCAAGT GTCAGGTGCA 
ATTGTTGATG GGGCCCCCAA TAGTGACCAT GGTAGTGAGC CACTTGTATT ACCAGGAAGC 
TATTCACTTA ATAGACAAGA ACAGAAGCCA CTTTCACAGG AAACTCTTAA TTATAATTCC 
CATAATTTAG TTGGAAGCAT GGGTGATGCA TGCAAATCCA TGGGCCAGAT GTCAGCACAA 
TGTCTAGATG TTTCTGGTAA TGGTTCATTT CATATCCAAA ATAGCTCATC AAATGAGATA 
GCTTCTGAAT TAGAGGCCAT CAAGAAAGAT CCAGCACCAT CACTGCAATT ACATCATATT 
ATGCCCAACA AGCAAAATGA GTCCAGTATA AGTCAATCAC ATGATGGGCA TCCAATTGCA 
TCACAAACTA ATGAATCTTG CCATTCACAT GATCCAAGAG TATCTCCACA TCCTAATGAT 
CAACAGTTAC AAAATGAAAG AACGGCCATG TTTCCTCTCA CCAGATTACC TGCACTACAC 
AGTTCGGTTA ATGAAATTCT AGCAAAACAC AAGTTTCACA ACAGTTTAGA GATGCAAAAT 
GAGCATCTCA GGCAAATCAA AGTCATGCAG AATTGCAATG GGACAAGTAA AGATGATTCT 
TTCATTAAGT CTACAAAAGA ATTTCAATCC AATCCTTTTT TAAAATCAGG CTGGTTAGAA 
CTTCGCCCTA ATCAGCGCCT AAAAGAAGGA TCTTCTGAAA CTTCTTGTGA AAATTTACTG 
AACTTACTTC TCCAGTCCCA GACTAATAAC AAGAAAAAGC TAAGACAATA CCCTGGAAAA 
CAAGGTTTAG CAAATAGAAA GTGTAACAGT CAAGATAGTG AAGAACATCT GCAGCCAAAT 
ATTTCTGAGC AGCAGCCAAA TTTCCCAAAG CACTTATCAC AAGATTCTCA ATACCAGCCA 
TACAGTCAAA AAACTAATAA TGGTCTGATG TGGCCTGATC TTGAACAAGC TTCTGAGGGC 
ACTCAGTTAG GTGATGTCCA ACATTTTTTG CAAGCACAGC CAAGTGAATA TATTGAAGAG 
CTGTTAAAAC AACATTTTGA TCAGTCACTC CGTAACTGTA ACCAAAATAG GGACAGTCCT 
CTTAACCACT TTAAACTTGA AGAAGGTGCT CAGGCAGCTA ATCAGTATCA AAAACACCAT 
CCATATCCTC AGCAATTTGA GATGGAACCT AAGCAAAGTT TAAGCAACAT GAATTCCCCC 
TTCAGTCAAA TTCAAGAAAA TAATACAGTT AACCCTAACC ATTCCTATCC TGACAAACAA 
ATATATCTGT CACAAAAAAC AGAAAATAGC TGTGGTTTTG CAGAGAACAA ACCTGACCCT 
TTGCAATACA TGCAGTATTA TCAAGCAAAT AGTACTGAGC AACAAGAAAT AAGCAATTAT 
GAGCATGATC TTTCGGCCCG AATAGCAAAG CAGACACATT GCCTGCCAGA GAGCTTTAGT 
TCATCCCCAC ATTTGCATGA TAAAATGGGA TATTATTCTC AATTTGAAAA CCAGGTTGAC 
TGTATTCCAA AGCATGCTGC TCTTAGGAAT CACTTGCTGC AACGAAGTAG ATCAGTTAAA 
GCTGATAGAT TGGTTAAAGC TGAGAAGAGT TCCCACTCTA ATGCCTGCAT GCATACACCT 
ATGGAAAGCA AAACTAAAAA AAACACAGAT AAAAAAATCA AACAAGAAAC TCGCCATTCA 
AGCACAGAGG CTGGACAACA AAAGAGTATT ATTGAAACAA TGGAACAGCA GATGAGACAT 
TATCCTGGTA AATCATCGTT TCATCGTCAA GTATCAAGTA TCAAATCTCC CAAGCATGTG 
AAGGTGGAGT CTTCAGGGCC TGTCACAGTC CTAACTACAC ACACTAATTT TTCTACACTA 
GACCAACAAA CTGTTCCTCC GCATGAGAAA ACACCAACCA AAAGAGCAGC AGGTTCTGCT 
CTACATAACT TTATAGAATC ACCCTCAAAT CTACTCAGTA CACCAGTAAA AAATCTTTTG 
GACACACCTA TAAAAACGCA GTATGATTTT CCATCATGCA GTTGTGTAGA TCAAATAATT 
GAAAAAGATG AAGGACCTTA TTATACACAT CTTGGTGCTG GACCCAACGT GGCAGCTATC 
AGAGAAATGA TGGAAGAAAG GTTTGGCCAA AAGGGGAATG CCATCAGAAT TGAGAGGGTT 
GTTTATACAG GAAAAGAAGG CAAAAGTGCT CAGGGATGTC CTATTGCTAA ATGGGTGATT 
CGGCGTAGTG GGACAGATGA GAAAATGCTG TGTTTGGTGA GGGAACGTGC TGGTCACTCC 
TGCGAAACAG CAGTGATTGT CATTCTGATC TTGGTTTGGG AAGGCATTTC ATTCTCTTTG 
GCAGACAGGC TTTACTCAGA GCTCACAGAA ACGCTAAATA AGTATGGAAC CTTAACAAAC 
CGACGCTGTG CACGTAACGA AGAACGTACC TGTGCATGCC AGGGTCTGGA CTCTGAAACC 
TGCGGTGCCT CCTTTTCCTT TGGGTGCTCA TGGAGTATGT ATTATAACGG GTGTAAATTC 
GCTAGAAGTA AAGTACCAAG AAAATTCAAA CTCCTTGGTG ATGATCCAAA ATCGGAAGAA 
AAGTTGGAAG CTAATTTACA GCACTTATCA ACACTGATGG CTCCAATTTA CAAGAAGCTT 
GCCCCTGATG CCTATCATAA CCAGATTGAG CATGAGCACA GAGCTCCAGA TTGCCGTCTT 
GGTTTGAAGG AAGGTCGACC ATTTTCTGGG GTTACTGCTT GCTTGGATTT TTGCGCTCAT 
TCCCACCGGG ACTTGCACAA CATGCAAAAT GGCAGCACAC TGGTTTGCAC TTTAACCAGA 
GAAGACAATC GTGAAAATGG AAAAATTCCA CAAGATGAGC AACTGCATGT CTTACCCCTT 
TATAAAGTAT CCAATGTGGA TGAGTTTGGC AGTTCTGAGA GTCAGGAAGA GAAGAAAAGA 
ACTGGTGCTA TTCAAGTTCT CACATCTTTC CGAAGGCAAG TGCGAATGCT AGCTGAGCCT 
GTAAAAACTT GCCGGCAAAA GAAGTTGGAA AAACAAGAAG CCAAGAAAGC AGCAGCAGAG 
AAGTTAAATG CCGCTTTAGA AAATGGTCAC AGCAAGAACG AAAAGGAGAA GTCCTCACGG 
CATAAAAACG CACATGTAGA AGCTTCTACG CAGGACAAAC AGCAAGCAGA ATTTTCTCGA 
ATGCCAGCCT TGCAACAAAA CACCCTTCTC AATATGAACC ATAGGAACTC TTTAAACCAT 
TATCCATCAT CCGCCCCTAC AAATCCTTAC ACACGAATTC CTAATCTGCC TGACCCATAT 
CAGAATAGTT TTTCTCCCTA CCCTGGATCA AGCCACATGA ATATTTTTCA AACCGCTGCA 
AGCTCATCAG TATCCTGTGC AAATAGCCCT ATGAATTCTT TTCAAGGATC CTTTAATCAA 
GGTAACTCTT GCCCCTCTTA TCCCAGTAAT GGAAATGCCT CCTTGCACAA CTGTCCCCCT 
TATCTTGACT CTTCACAGCA ACAATCCATG GATTATTACA AGTTTAAAAA CCAAGATTCA 
ATGGCTAAAT TAGGTGTGCC CCCAATTCAA TCATTGTACC AGCAGGGGTT TGCTAATAAT 
CAGGCTTATG GTAACAAAAT GTTTAGTTAT GGAAACCAAA GAGTGCAAGC TGATGATTTC 
AGTGGCTGTA ATGCTTATCC AGCACCTCAG GCTGATATAA TGAATCCAGC CACAAGTTCA 
CGGCCTATTA CCAGTAATCA AGACTTCTCT TCTGCGACTA AGAATGGTGA ATATCCTCTT 
CCACACTCCT TCCAGGGTCA TGAAAGCAAC ATGTCATCAA GTATATTGGG AGGCAGAGCT 
GGTTCTCTGC CATTCCCTGG TAAACAGAGT GAAATGCATT CACGCACAGC AAATGGAACA 
TCGGAAATGC TTCCAGGTCT TAACCATGAT AGAACTAATT TTGGAGGCGC AGGCAGAACT 
AATGGTGCTA ATGTTCAGGG AAAGCTACCG TACACCTTAC CTCCTGTACC AGAAGAGCCT 
GCAGAAGTTT GGTCAGACAG TGAACAGAAT TTTCTTGATC CTGATATAGG AGGTGTAGCA 
GTTTGCCCAA CTCATGGGTC AATCTTGATT GAATGTGCAA AGCGTGAGCT CCACGCAACA 
ACACCTTTAA AGAATCCCAA CAGAAATCAC CCTACCAGAA TATCTTTGGT GTTCTACCAA 
CACAAGAGCA TGAACGAGCC AAAGCATGGC CTGGCATTGT GGGAAGCAAA AATGGCAGGC 
AAAGCCAGGG AAAAGGAAGA AGATTGTGAA AGGTATGGTC CAGATTATGT GGCTCCTAAA 
TCTTACAACA GGAAAGCAAA GCGGGAGCCT GTGGAGCCAA TACATGAACC TGCTGAACCA 
ACTTATCTTC GTTTCATCAA GTCACTTACG CAGAGGACGA TGTCAATAAC CACAGACTCT 
CAAGTCACTA CATCTCCATA TGCCTTAACA CGGGTTACAG GTCCTTATAA CAGATATATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810969.1
CDS	1075..6357
Translation	MEEDRTNHDEGNRLSPTLLYSSSACQNKNGLLQNGNLPTDKDLLVNGDICLPTKNNYNVAYMKGSQEHGASALIHDNKAYPNHIQNGGIKRTYSEPLFSESQLKKVRTDEINGENKGDQRQNNIQASVSEFCNEDQSVRIFQPVNSPALQVPNEQKKDLNYSNRDIVSCLKDKTVPMCNGATASVSSMENVNNELLEKLPSQYYPEHVSTVVHNNTSLANAINTLNDASNDLSTLATHPSHTSGQMTPQQTSVLPQVSGAIVDGAPNSDHGSEPLVLPGSYSLNRQEQKPLSQETLNYNSHNLVGSMGDACKSMGQMSAQCLDVSGNGSFHIQNSSSNEIASELEAIKKDPAPSLQLHHIMPNKQNESSISQSHDGHPIASQTNESCHSHDPRVSPHPNDQQLQNERTAMFPLTRLPALHSSVNEILAKHKFHNSLEMQNEHLRQIKVMQNCNGTSKDDSFIKSTKEFQSNPFLKSGWLELRPNQRLKEGSSETSCENLLNLLLQSQTNNKKKLRQYPGKQGLANRKCNSQDSEEHLQPNISEQQPNFPKHLSQDSQYQPYSQKTNNGLMWPDLEQASEGTQLGDVQHFLQAQPSEYIEELLKQHFDQSLRNCNQNRDSPLNHFKLEEGAQAANQYQKHHPYPQQFEMEPKQSLSNMNSPFSQIQENNTVNPNHSYPDKQIYLSQKTENSCGFAENKPDPLQYMQYYQANSTEQQEISNYEHDLSARIAKQTHCLPESFSSSPHLHDKMGYYSQFENQVDCIPKHAALRNHLLQRSRSVKADRLVKAEKSSHSNACMHTPMESKTKKNTDKKIKQETRHSSTEAGQQKSIIETMEQQMRHYPGKSSFHRQVSSIKSPKHVKVESSGPVTVLTTHTNFSTLDQQTVPPHEKTPTKRAAGSALHNFIESPSNLLSTPVKNLLDTPIKTQYDFPSCSCVDQIIEKDEGPYYTHLGAGPNVAAIREMMEERFGQKGNAIRIERVVYTGKEGKSAQGCPIAKWVIRRSGTDEKMLCLVRERAGHSCETAVIVILILVWEGISFSLADRLYSELTETLNKYGTLTNRRCARNEERTCACQGLDSETCGASFSFGCSWSMYYNGCKFARSKVPRKFKLLGDDPKSEEKLEANLQHLSTLMAPIYKKLAPDAYHNQIEHEHRAPDCRLGLKEGRPFSGVTACLDFCAHSHRDLHNMQNGSTLVCTLTREDNRENGKIPQDEQLHVLPLYKVSNVDEFGSSESQEEKKRTGAIQVLTSFRRQVRMLAEPVKTCRQKKLEKQEAKKAAAEKLNAALENGHSKNEKEKSSRHKNAHVEASTQDKQQAEFSRMPALQQNTLLNMNHRNSLNHYPSSAPTNPYTRIPNLPDPYQNSFSPYPGSSHMNIFQTAASSSVSCANSPMNSFQGSFNQGNSCPSYPSNGNASLHNCPPYLDSSQQQSMDYYKFKNQDSMAKLGVPPIQSLYQQGFANNQAYGNKMFSYGNQRVQADDFSGCNAYPAPQADIMNPATSSRPITSNQDFSSATKNGEYPLPHSFQGHESNMSSSILGGRAGSLPFPGKQSEMHSRTANGTSEMLPGLNHDRTNFGGAGRTNGANVQGKLPYTLPPVPEEPAEVWSDSEQNFLDPDIGGVAVCPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAGKAREKEEDCERYGPDYVAPKSYNRKAKREPVEPIHEPAEPTYLRFIKSLTQRTMSITTDSQVTTSPYALTRVTGPYNRYI

Target ORF information:

RefSeq Version	XM_012955515.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955515.3

ORF Insert Sequence:

ATGGAAGAGG ACAGAACCAA CCATGACGAG GGCAATAGAT TGAGTCCAAC CCTATTATAT 
TCTTCGTCAG CTTGCCAAAA CAAGAACGGT CTATTACAAA ATGGGAACCT GCCAACAGAC 
AAAGACCTTC TAGTTAATGG GGACATATGT TTACCAACCA AAAACAACTA TAATGTTGCT 
TACATGAAGG GAAGTCAAGA ACATGGTGCT TCTGCTCTCA TCCATGACAA CAAAGCTTAT 
CCCAACCACA TACAAAATGG TGGAATAAAG CGCACATATA GTGAACCGTT GTTTTCTGAA 
TCCCAGTTGA AAAAGGTCAG AACAGATGAG ATTAATGGAG AAAATAAGGG TGACCAAAGG 
CAAAATAATA TTCAAGCAAG TGTCTCTGAG TTCTGCAATG AAGATCAGTC AGTAAGAATT 
TTCCAACCAG TTAATTCACC AGCTCTTCAG GTTCCAAATG AGCAGAAGAA AGACCTTAAT 
TACAGCAACA GAGACATTGT CTCATGTCTT AAAGACAAGA CAGTGCCAAT GTGTAATGGT 
GCTACTGCTT CTGTATCTTC CATGGAAAAC GTGAATAATG AGCTCCTGGA AAAACTGCCT 
TCTCAATATT ATCCTGAACA TGTTTCTACT GTGGTGCATA ATAACACATC TCTTGCAAAT 
GCCATTAATA CGCTAAATGA TGCTAGCAAT GATTTGTCTA CTCTGGCAAC TCATCCGTCA 
CATACCTCAG GGCAGATGAC TCCTCAACAA ACTTCGGTGC TGCCTCAAGT GTCAGGTGCA 
ATTGTTGATG GGGCCCCCAA TAGTGACCAT GGTAGTGAGC CACTTGTATT ACCAGGAAGC 
TATTCACTTA ATAGACAAGA ACAGAAGCCA CTTTCACAGG AAACTCTTAA TTATAATTCC 
CATAATTTAG TTGGAAGCAT GGGTGATGCA TGCAAATCCA TGGGCCAGAT GTCAGCACAA 
TGTCTAGATG TTTCTGGTAA TGGTTCATTT CATATCCAAA ATAGCTCATC AAATGAGATA 
GCTTCTGAAT TAGAGGCCAT CAAGAAAGAT CCAGCACCAT CACTGCAATT ACATCATATT 
ATGCCCAACA AGCAAAATGA GTCCAGTATA AGTCAATCAC ATGATGGGCA TCCAATTGCA 
TCACAAACTA ATGAATCTTG CCATTCACAT GATCCAAGAG TATCTCCACA TCCTAATGAT 
CAACAGTTAC AAAATGAAAG AACGGCCATG TTTCCTCTCA CCAGATTACC TGCACTACAC 
AGTTCGGTTA ATGAAATTCT AGCAAAACAC AAGTTTCACA ACAGTTTAGA GATGCAAAAT 
GAGCATCTCA GGCAAATCAA AGTCATGCAG AATTGCAATG GGACAAGTAA AGATGATTCT 
TTCATTAAGT CTACAAAAGA ATTTCAATCC AATCCTTTTT TAAAATCAGG CTGGTTAGAA 
CTTCGCCCTA ATCAGCGCCT AAAAGAAGGA TCTTCTGAAA CTTCTTGTGA AAATTTACTG 
AACTTACTTC TCCAGTCCCA GACTAATAAC AAGAAAAAGC TAAGACAATA CCCTGGAAAA 
CAAGGTTTAG CAAATAGAAA GTGTAACAGT CAAGATAGTG AAGAACATCT GCAGCCAAAT 
ATTTCTGAGC AGCAGCCAAA TTTCCCAAAG CACTTATCAC AAGATTCTCA ATACCAGCCA 
TACAGTCAAA AAACTAATAA TGGTCTGATG TGGCCTGATC TTGAACAAGC TTCTGAGGGC 
ACTCAGTTAG GTGATGTCCA ACATTTTTTG CAAGCACAGC CAAGTGAATA TATTGAAGAG 
CTGTTAAAAC AACATTTTGA TCAGTCACTC CGTAACTGTA ACCAAAATAG GGACAGTCCT 
CTTAACCACT TTAAACTTGA AGAAGGTGCT CAGGCAGCTA ATCAGTATCA AAAACACCAT 
CCATATCCTC AGCAATTTGA GATGGAACCT AAGCAAAGTT TAAGCAACAT GAATTCCCCC 
TTCAGTCAAA TTCAAGAAAA TAATACAGTT AACCCTAACC ATTCCTATCC TGACAAACAA 
ATATATCTGT CACAAAAAAC AGAAAATAGC TGTGGTTTTG CAGAGAACAA ACCTGACCCT 
TTGCAATACA TGCAGTATTA TCAAGCAAAT AGTACTGAGC AACAAGAAAT AAGCAATTAT 
GAGCATGATC TTTCGGCCCG AATAGCAAAG CAGACACATT GCCTGCCAGA GAGCTTTAGT 
TCATCCCCAC ATTTGCATGA TAAAATGGGA TATTATTCTC AATTTGAAAA CCAGGTTGAC 
TGTATTCCAA AGCATGCTGC TCTTAGGAAT CACTTGCTGC AACGAAGTAG ATCAGTTAAA 
GCTGATAGAT TGGTTAAAGC TGAGAAGAGT TCCCACTCTA ATGCCTGCAT GCATACACCT 
ATGGAAAGCA AAACTAAAAA AAACACAGAT AAAAAAATCA AACAAGAAAC TCGCCATTCA 
AGCACAGAGG CTGGACAACA AAAGAGTATT ATTGAAACAA TGGAACAGCA GATGAGACAT 
TATCCTGGTA AATCATCGTT TCATCGTCAA GTATCAAGTA TCAAATCTCC CAAGCATGTG 
AAGGTGGAGT CTTCAGGGCC TGTCACAGTC CTAACTACAC ACACTAATTT TTCTACACTA 
GACCAACAAA CTGTTCCTCC GCATGAGAAA ACACCAACCA AAAGAGCAGC AGGTTCTGCT 
CTACATAACT TTATAGAATC ACCCTCAAAT CTACTCAGTA CACCAGTAAA AAATCTTTTG 
GACACACCTA TAAAAACGCA GTATGATTTT CCATCATGCA GTTGTGTAGA TCAAATAATT 
GAAAAAGATG AAGGACCTTA TTATACACAT CTTGGTGCTG GACCCAACGT GGCAGCTATC 
AGAGAAATGA TGGAAGAAAG GTTTGGCCAA AAGGGGAATG CCATCAGAAT TGAGAGGGTT 
GTTTATACAG GAAAAGAAGG CAAAAGTGCT CAGGGATGTC CTATTGCTAA ATGGGTGATT 
CGGCGTAGTG GGACAGATGA GAAAATGCTG TGTTTGGTGA GGGAACGTGC TGGTCACTCC 
TGCGAAACAG CAGTGATTGT CATTCTGATC TTGGTTTGGG AAGGCATTTC ATTCTCTTTG 
GCAGACAGGC TTTACTCAGA GCTCACAGAA ACGCTAAATA AGTATGGAAC CTTAACAAAC 
CGACGCTGTG CACGTAACGA AGAACGTACC TGTGCATGCC AGGGTCTGGA CTCTGAAACC 
TGCGGTGCCT CCTTTTCCTT TGGGTGCTCA TGGAGTATGT ATTATAACGG GTGTAAATTC 
GCTAGAAGTA AAGTACCAAG AAAATTCAAA CTCCTTGGTG ATGATCCAAA ATCGGAAGAA 
AAGTTGGAAG CTAATTTACA GCACTTATCA ACACTGATGG CTCCAATTTA CAAGAAGCTT 
GCCCCTGATG CCTATCATAA CCAGATTGAG CATGAGCACA GAGCTCCAGA TTGCCGTCTT 
GGTTTGAAGG AAGGTCGACC ATTTTCTGGG GTTACTGCTT GCTTGGATTT TTGCGCTCAT 
TCCCACCGGG ACTTGCACAA CATGCAAAAT GGCAGCACAC TGGTTTGCAC TTTAACCAGA 
GAAGACAATC GTGAAAATGG AAAAATTCCA CAAGATGAGC AACTGCATGT CTTACCCCTT 
TATAAAGTAT CCAATGTGGA TGAGTTTGGC AGTTCTGAGA GTCAGGAAGA GAAGAAAAGA 
ACTGGTGCTA TTCAAGTTCT CACATCTTTC CGAAGGCAAG TGCGAATGCT AGCTGAGCCT 
GTAAAAACTT GCCGGCAAAA GAAGTTGGAA AAACAAGAAG CCAAGAAAGC AGCAGCAGAG 
AAGTTAAATG CCGCTTTAGA AAATGGTCAC AGCAAGAACG AAAAGGAGAA GTCCTCACGG 
CATAAAAACG CACATGTAGA AGCTTCTACG CAGGACAAAC AGCAAGCAGA ATTTTCTCGA 
ATGCCAGCCT TGCAACAAAA CACCCTTCTC AATATGAACC ATAGGAACTC TTTAAACCAT 
TATCCATCAT CCGCCCCTAC AAATCCTTAC ACACGAATTC CTAATCTGCC TGACCCATAT 
CAGAATAGTT TTTCTCCCTA CCCTGGATCA AGCCACATGA ATATTTTTCA AACCGCTGCA 
AGCTCATCAG TATCCTGTGC AAATAGCCCT ATGAATTCTT TTCAAGGATC CTTTAATCAA 
GGTAACTCTT GCCCCTCTTA TCCCAGTAAT GGAAATGCCT CCTTGCACAA CTGTCCCCCT 
TATCTTGACT CTTCACAGCA ACAATCCATG GATTATTACA AGTTTAAAAA CCAAGATTCA 
ATGGCTAAAT TAGGTGTGCC CCCAATTCAA TCATTGTACC AGCAGGGGTT TGCTAATAAT 
CAGGCTTATG GTAACAAAAT GTTTAGTTAT GGAAACCAAA GAGTGCAAGC TGATGATTTC 
AGTGGCTGTA ATGCTTATCC AGCACCTCAG GCTGATATAA TGAATCCAGC CACAAGTTCA 
CGGCCTATTA CCAGTAATCA AGACTTCTCT TCTGCGACTA AGAATGGTGA ATATCCTCTT 
CCACACTCCT TCCAGGGTCA TGAAAGCAAC ATGTCATCAA GTATATTGGG AGGCAGAGCT 
GGTTCTCTGC CATTCCCTGG TAAACAGAGT GAAATGCATT CACGCACAGC AAATGGAACA 
TCGGAAATGC TTCCAGGTCT TAACCATGAT AGAACTAATT TTGGAGGCGC AGGCAGAACT 
AATGGTGCTA ATGTTCAGGG AAAGCTACCG TACACCTTAC CTCCTGTACC AGAAGAGCCT 
GCAGAAGTTT GGTCAGACAG TGAACAGAAT TTTCTTGATC CTGATATAGG AGGTGTAGCA 
GTTTGCCCAA CTCATGGGTC AATCTTGATT GAATGTGCAA AGCGTGAGCT CCACGCAACA 
ACACCTTTAA AGAATCCCAA CAGAAATCAC CCTACCAGAA TATCTTTGGT GTTCTACCAA 
CACAAGAGCA TGAACGAGCC AAAGCATGGC CTGGCATTGT GGGAAGCAAA AATGGCAGGC 
AAAGCCAGGG AAAAGGAAGA AGATTGTGAA AGGTATGGTC CAGATTATGT GGCTCCTAAA 
TCTTACAACA GGAAAGCAAA GCGGGAGCCT GTGGAGCCAA TACATGAACC TGCTGAACCA 
ACTTATCTTC GTTTCATCAA GTCACTTACG CAGAGGACGA TGTCAATAAC CACAGACTCT 
CAAGTCACTA CATCTCCATA TGCCTTAACA CGGGTTACAG GTCCTTATAA CAGATATATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet2 ( XM_002934777.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934823.2 Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X1, mRNA.

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CloneID	OXa04496
Clone ID Related Accession (Same CDS sequence)	XM_002934777.4
Accession Version	XM_002934777.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	methylcytosine dioxygenase TET2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934777.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCCA AAGCTAACCT ATATGTGGAT TGCTACTTTG TGCTTCTGCA GATACGTTTA 
CAGGTCAGTC TTGTGGATGG CCCGGGAGCA GGCCAGATGG AAGAGGACAG AACCAACCAT 
GACGAGGGCA ATAGATTGAG TCCAACCCTA TTATATTCTT CGTCAGCTTG CCAAAACAAG 
AACGGTCTAT TACAAAATGG GAACCTGCCA ACAGACAAAG ACCTTCTAGT TAATGGGGAC 
ATATGTTTAC CAACCAAAAA CAACTATAAT GTTGCTTACA TGAAGGGAAG TCAAGAACAT 
GGTGCTTCTG CTCTCATCCA TGACAACAAA GCTTATCCCA ACCACATACA AAATGGTGGA 
ATAAAGCGCA CATATAGTGA ACCGTTGTTT TCTGAATCCC AGTTGAAAAA GGTCAGAACA 
GATGAGATTA ATGGAGAAAA TAAGGGTGAC CAAAGGCAAA ATAATATTCA AGCAAGTGTC 
TCTGAGTTCT GCAATGAAGA TCAGTCAGTA AGAATTTTCC AACCAGTTAA TTCACCAGCT 
CTTCAGGTTC CAAATGAGCA GAAGAAAGAC CTTAATTACA GCAACAGAGA CATTGTCTCA 
TGTCTTAAAG ACAAGACAGT GCCAATGTGT AATGGTGCTA CTGCTTCTGT ATCTTCCATG 
GAAAACGTGA ATAATGAGCT CCTGGAAAAA CTGCCTTCTC AATATTATCC TGAACATGTT 
TCTACTGTGG TGCATAATAA CACATCTCTT GCAAATGCCA TTAATACGCT AAATGATGCT 
AGCAATGATT TGTCTACTCT GGCAACTCAT CCGTCACATA CCTCAGGGCA GATGACTCCT 
CAACAAACTT CGGTGCTGCC TCAAGTGTCA GGTGCAATTG TTGATGGGGC CCCCAATAGT 
GACCATGGTA GTGAGCCACT TGTATTACCA GGAAGCTATT CACTTAATAG ACAAGAACAG 
AAGCCACTTT CACAGGAAAC TCTTAATTAT AATTCCCATA ATTTAGTTGG AAGCATGGGT 
GATGCATGCA AATCCATGGG CCAGATGTCA GCACAATGTC TAGATGTTTC TGGTAATGGT 
TCATTTCATA TCCAAAATAG CTCATCAAAT GAGATAGCTT CTGAATTAGA GGCCATCAAG 
AAAGATCCAG CACCATCACT GCAATTACAT CATATTATGC CCAACAAGCA AAATGAGTCC 
AGTATAAGTC AATCACATGA TGGGCATCCA ATTGCATCAC AAACTAATGA ATCTTGCCAT 
TCACATGATC CAAGAGTATC TCCACATCCT AATGATCAAC AGTTACAAAA TGAAAGAACG 
GCCATGTTTC CTCTCACCAG ATTACCTGCA CTACACAGTT CGGTTAATGA AATTCTAGCA 
AAACACAAGT TTCACAACAG TTTAGAGATG CAAAATGAGC ATCTCAGGCA AATCAAAGTC 
ATGCAGAATT GCAATGGGAC AAGTAAAGAT GATTCTTTCA TTAAGTCTAC AAAAGAATTT 
CAATCCAATC CTTTTTTAAA ATCAGGCTGG TTAGAACTTC GCCCTAATCA GCGCCTAAAA 
GAAGGATCTT CTGAAACTTC TTGTGAAAAT TTACTGAACT TACTTCTCCA GTCCCAGACT 
AATAACAAGA AAAAGCTAAG ACAATACCCT GGAAAACAAG GTTTAGCAAA TAGAAAGTGT 
AACAGTCAAG ATAGTGAAGA ACATCTGCAG CCAAATATTT CTGAGCAGCA GCCAAATTTC 
CCAAAGCACT TATCACAAGA TTCTCAATAC CAGCCATACA GTCAAAAAAC TAATAATGGT 
CTGATGTGGC CTGATCTTGA ACAAGCTTCT GAGGGCACTC AGTTAGGTGA TGTCCAACAT 
TTTTTGCAAG CACAGCCAAG TGAATATATT GAAGAGCTGT TAAAACAACA TTTTGATCAG 
TCACTCCGTA ACTGTAACCA AAATAGGGAC AGTCCTCTTA ACCACTTTAA ACTTGAAGAA 
GGTGCTCAGG CAGCTAATCA GTATCAAAAA CACCATCCAT ATCCTCAGCA ATTTGAGATG 
GAACCTAAGC AAAGTTTAAG CAACATGAAT TCCCCCTTCA GTCAAATTCA AGAAAATAAT 
ACAGTTAACC CTAACCATTC CTATCCTGAC AAACAAATAT ATCTGTCACA AAAAACAGAA 
AATAGCTGTG GTTTTGCAGA GAACAAACCT GACCCTTTGC AATACATGCA GTATTATCAA 
GCAAATAGTA CTGAGCAACA AGAAATAAGC AATTATGAGC ACGATCTTTC GGCCCGAATA 
GCAAAGCAGA CACATTGCCT GCCAGAGAGC TTTAGTTCAT CCCCACATTT GCATGATAAA 
ATGGGATATT ATTCTCAATT TGAAAACCAG GTTGACTGTA TTCCAAAGCA TGCTGCTCTT 
AGGAATCACT TGCTGCAACG AAGTAGATCA GTTAAAGCTG ATAGATTGGT TAAAGCTGAG 
AAGAGTTCCC ACTCTAATGC CTGCATGCAT ACACCTATGG AAAGCAAAAC TAAAAAAAAC 
ACAGATAAAA AAATCAAACA AGAAACTCGC CATTCAAGCA CAGAGGCTGG ACAACAAAAG 
AGTATTATTG AAACAATGGA ACAGCAGATG AGACATTATC CTGGTAAATC ATCGTTTCAT 
CGTCAAGTAT CAAGTATCAA ATCTCCCAAG CATGTGAAGG TGGAGTCTTC AGGGCCTGTC 
ACAGTCCTAA CTACACACAC TAATTTTTCT ACACTAGACC AACAAACTGT TCCTCCGCAT 
GAGAAAACAC CAACCAAAAG AGCAGCAGGT TCTGCTCTAC ATAACTTTAT AGAATCACCC 
TCAAATCTAC TCAGTACACC AGTAAAAAAT CTTTTGGACA CACCTATAAA AACGCAGTAT 
GATTTTCCAT CATGCAGTTG TGTAGATCAA ATAATTGAAA AAGATGAAGG ACCTTATTAT 
ACACATCTTG GTGCTGGACC CAATGTGGCA GCTATCAGAG AAATGATGGA AGAAAGGTTT 
GGCCAAAAGG GGAATGCCAT CAGAATTGAG AGGGTTGTTT ATACAGGAAA AGAAGGCAAA 
AGTGCTCAGG GATGTCCTAT TGCTAAATGG GTGATTCGGC GTAGTGGGAC AGATGAGAAA 
ATGCTGTGTT TGGTGAGGGA ACGTGCTGGT CACTCCTGCG AAACAGCAGT GATTGTCATT 
CTGATCTTGG TTTGGGAAGG CATTTCATTC TCTTTGGCAG ACAGGCTTTA CTCAGAGCTC 
ACAGAAACGC TAAATAAGTA TGGAACCTTA ACAAACCGAC GCTGTGCACG TAACGAAGAA 
CGTACCTGTG CATGCCAGGG TCTGGACTCT GAAACCTGCG GTGCCTCCTT TTCCTTTGGG 
TGCTCATGGA GTATGTATTA TAACGGGTGT AAATTCGCTA GAAGTAAAGT ACCAAGAAAA 
TTCAAACTCC TTGGTGATGA TCCAAAATCG GAAGAAAAGT TGGAAGCTAA TTTACAGCAC 
TTATCAACAC TGATGGCTCC AATTTACAAG AAGCTTGCCC CTGATGCCTA TCATAACCAG 
ATTGAGCATG AGCACAGAGC TCCAGATTGC CGTCTTGGTT TGAAGGAAGG TCGACCATTT 
TCTGGGGTTA CTGCTTGCTT GGATTTTTGC GCTCATTCCC ACCGGGACTT GCACAACATG 
CAAAATGGCA GCACACTGGT TTGCACTTTA ACCAGAGAAG ACAATCGTGA AAATGGAAAA 
ATTCCACAAG ATGAGCAACT GCATGTCTTA CCCCTTTATA AAGTATCCAA TGTGGATGAG 
TTTGGCAGTT CTGAGAGTCA GGAAGAGAAG AAAAGAACTG GTGCTATTCA AGTTCTCACA 
TCTTTCCGAA GGCAAGTGCG AATGCTAGCT GAGCCTGTAA AAACTTGCCG GCAAAAGAAG 
TTGGAAAAAC AAGAAGCCAA GAAAGCAGCA GCAGAGAAGT TAAATGCCGC TTTAGAAAAT 
GGTCACAGCA AGAACGAAAA GGAGAAGTCC TCACGGCATA AAAACGCACA TGTAGAAGCT 
TCTACGCAGG ACAAACAGCA AGCAGAATTT TCTCGAATGC CAGCCTTGCA ACAAAACACC 
CTTCTCAATA TGAACCATAG GAACTCTTTA AACCATTATC CATCATCCGC CCCTACAAAT 
CCTTACACAC GAATTCCTAA TCTGCCTGAC CCATATCAGA ATAGTTTTTC TCCCTACCCT 
GGATCAAGCC ACATGAATAT TTTTCAAACC GCTGCAAGCT CATCAGTATC CTGTGCAAAT 
AGCCCTATGA ATTCTTTTCA AGGATCCTTT AATCAAGGTA ACTCTTGCCC CTCTTATCCC 
AGTAATGGAA ATGCCTCCTT GCACAACTGT CCCCCTTATC TTGACTCTTC ACAGCAACAA 
TCCATGGATT ATTACAAGTT TAAAAACCAA GATTCAATGG CTAAATTAGG TGTGCCCCCA 
ATTCAATCAT TGTACCAGCA GGGGTTTGCT AATAATCAGG CTTATGGTAA CAAAATGTTT 
AGTTATGGAA ACCAAAGAGT GCAAGCTGAT GATTTCAGTG GCTGTAATGC TTATCCAGCA 
CCTCAGGCTG ATATAATGAA TCCAGCCACA AGTTCACGGC CTATTACCAG TAATCAAGAC 
TTCTCTTCTG CGACTAAGAA TGGTGAATAT CCTCTTCCAC ACTCCTTCCA GGGTCATGAA 
AGCAACATGT CATCAAGTAT ATTGGGAGGC AGAGCTGGTT CTCTGCCATT CCCTGGTAAA 
CAGAGTGAAA TGCATTCACG CACAGCAAAT GGAACATCGG AAATGCTTCC AGGTCTTAAC 
CATGATAGAA CTAATTTTGG AGGCGCAGGC AGAACTAATG GTGCTAATGT TCAGGGAAAG 
CTACCGTACA CCTTACCTCC TGTACCAGAA GAGCCTGCAG AAGTTTGGTC AGACAGTGAA 
CAGAATTTTC TTGATCCTGA TATAGGAGGT GTAGCAGTTT GCCCAACTCA TGGGTCAATC 
TTGATTGAAT GTGCAAAGCG TGAGCTCCAC GCAACAACAC CTTTAAAGAA TCCCAACAGA 
AATCACCCTA CCAGAATATC TTTGGTGTTC TACCAACACA AGAGCATGAA CGAGCCAAAG 
CATGGCCTGG CATTGTGGGA AGCAAAAATG GCAGGCAAAG CCAGGGAAAA GGAAGAAGAT 
TGTGAAAGGT ATGGTCCAGA TTATGTGGCT CCTAAATCTT ACAACAGGAA AGCAAAGCGG 
GAGCCTGTGG AGCCAATACA TGAACCTGCT GAACCAACTT ATCTTCGTTT CATCAAGTCA 
CTTACGCAGA GGACGATGTC AATAACCACA GACTCTCAAG TCACTACATC TCCATATGCC 
TTAACACGGG TTACAGGTCC TTATAACAGA TATATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934823.2
CDS	290..5668
Translation	MTPKANLYVDCYFVLLQIRLQVSLVDGPGAGQMEEDRTNHDEGNRLSPTLLYSSSACQNKNGLLQNGNLPTDKDLLVNGDICLPTKNNYNVAYMKGSQEHGASALIHDNKAYPNHIQNGGIKRTYSEPLFSESQLKKVRTDEINGENKGDQRQNNIQASVSEFCNEDQSVRIFQPVNSPALQVPNEQKKDLNYSNRDIVSCLKDKTVPMCNGATASVSSMENVNNELLEKLPSQYYPEHVSTVVHNNTSLANAINTLNDASNDLSTLATHPSHTSGQMTPQQTSVLPQVSGAIVDGAPNSDHGSEPLVLPGSYSLNRQEQKPLSQETLNYNSHNLVGSMGDACKSMGQMSAQCLDVSGNGSFHIQNSSSNEIASELEAIKKDPAPSLQLHHIMPNKQNESSISQSHDGHPIASQTNESCHSHDPRVSPHPNDQQLQNERTAMFPLTRLPALHSSVNEILAKHKFHNSLEMQNEHLRQIKVMQNCNGTSKDDSFIKSTKEFQSNPFLKSGWLELRPNQRLKEGSSETSCENLLNLLLQSQTNNKKKLRQYPGKQGLANRKCNSQDSEEHLQPNISEQQPNFPKHLSQDSQYQPYSQKTNNGLMWPDLEQASEGTQLGDVQHFLQAQPSEYIEELLKQHFDQSLRNCNQNRDSPLNHFKLEEGAQAANQYQKHHPYPQQFEMEPKQSLSNMNSPFSQIQENNTVNPNHSYPDKQIYLSQKTENSCGFAENKPDPLQYMQYYQANSTEQQEISNYEHDLSARIAKQTHCLPESFSSSPHLHDKMGYYSQFENQVDCIPKHAALRNHLLQRSRSVKADRLVKAEKSSHSNACMHTPMESKTKKNTDKKIKQETRHSSTEAGQQKSIIETMEQQMRHYPGKSSFHRQVSSIKSPKHVKVESSGPVTVLTTHTNFSTLDQQTVPPHEKTPTKRAAGSALHNFIESPSNLLSTPVKNLLDTPIKTQYDFPSCSCVDQIIEKDEGPYYTHLGAGPNVAAIREMMEERFGQKGNAIRIERVVYTGKEGKSAQGCPIAKWVIRRSGTDEKMLCLVRERAGHSCETAVIVILILVWEGISFSLADRLYSELTETLNKYGTLTNRRCARNEERTCACQGLDSETCGASFSFGCSWSMYYNGCKFARSKVPRKFKLLGDDPKSEEKLEANLQHLSTLMAPIYKKLAPDAYHNQIEHEHRAPDCRLGLKEGRPFSGVTACLDFCAHSHRDLHNMQNGSTLVCTLTREDNRENGKIPQDEQLHVLPLYKVSNVDEFGSSESQEEKKRTGAIQVLTSFRRQVRMLAEPVKTCRQKKLEKQEAKKAAAEKLNAALENGHSKNEKEKSSRHKNAHVEASTQDKQQAEFSRMPALQQNTLLNMNHRNSLNHYPSSAPTNPYTRIPNLPDPYQNSFSPYPGSSHMNIFQTAASSSVSCANSPMNSFQGSFNQGNSCPSYPSNGNASLHNCPPYLDSSQQQSMDYYKFKNQDSMAKLGVPPIQSLYQQGFANNQAYGNKMFSYGNQRVQADDFSGCNAYPAPQADIMNPATSSRPITSNQDFSSATKNGEYPLPHSFQGHESNMSSSILGGRAGSLPFPGKQSEMHSRTANGTSEMLPGLNHDRTNFGGAGRTNGANVQGKLPYTLPPVPEEPAEVWSDSEQNFLDPDIGGVAVCPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAGKAREKEEDCERYGPDYVAPKSYNRKAKREPVEPIHEPAEPTYLRFIKSLTQRTMSITTDSQVTTSPYALTRVTGPYNRYI

Target ORF information:

RefSeq Version	XM_002934777.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934777.4

ORF Insert Sequence:

ATGACCCCCA AAGCTAACCT ATATGTGGAT TGCTACTTTG TGCTTCTGCA GATACGTTTA 
CAGGTCAGTC TTGTGGATGG CCCGGGAGCA GGCCAGATGG AAGAGGACAG AACCAACCAT 
GACGAGGGCA ATAGATTGAG TCCAACCCTA TTATATTCTT CGTCAGCTTG CCAAAACAAG 
AACGGTCTAT TACAAAATGG GAACCTGCCA ACAGACAAAG ACCTTCTAGT TAATGGGGAC 
ATATGTTTAC CAACCAAAAA CAACTATAAT GTTGCTTACA TGAAGGGAAG TCAAGAACAT 
GGTGCTTCTG CTCTCATCCA TGACAACAAA GCTTATCCCA ACCACATACA AAATGGTGGA 
ATAAAGCGCA CATATAGTGA ACCGTTGTTT TCTGAATCCC AGTTGAAAAA GGTCAGAACA 
GATGAGATTA ATGGAGAAAA TAAGGGTGAC CAAAGGCAAA ATAATATTCA AGCAAGTGTC 
TCTGAGTTCT GCAATGAAGA TCAGTCAGTA AGAATTTTCC AACCAGTTAA TTCACCAGCT 
CTTCAGGTTC CAAATGAGCA GAAGAAAGAC CTTAATTACA GCAACAGAGA CATTGTCTCA 
TGTCTTAAAG ACAAGACAGT GCCAATGTGT AATGGTGCTA CTGCTTCTGT ATCTTCCATG 
GAAAACGTGA ATAATGAGCT CCTGGAAAAA CTGCCTTCTC AATATTATCC TGAACATGTT 
TCTACTGTGG TGCATAATAA CACATCTCTT GCAAATGCCA TTAATACGCT AAATGATGCT 
AGCAATGATT TGTCTACTCT GGCAACTCAT CCGTCACATA CCTCAGGGCA GATGACTCCT 
CAACAAACTT CGGTGCTGCC TCAAGTGTCA GGTGCAATTG TTGATGGGGC CCCCAATAGT 
GACCATGGTA GTGAGCCACT TGTATTACCA GGAAGCTATT CACTTAATAG ACAAGAACAG 
AAGCCACTTT CACAGGAAAC TCTTAATTAT AATTCCCATA ATTTAGTTGG AAGCATGGGT 
GATGCATGCA AATCCATGGG CCAGATGTCA GCACAATGTC TAGATGTTTC TGGTAATGGT 
TCATTTCATA TCCAAAATAG CTCATCAAAT GAGATAGCTT CTGAATTAGA GGCCATCAAG 
AAAGATCCAG CACCATCACT GCAATTACAT CATATTATGC CCAACAAGCA AAATGAGTCC 
AGTATAAGTC AATCACATGA TGGGCATCCA ATTGCATCAC AAACTAATGA ATCTTGCCAT 
TCACATGATC CAAGAGTATC TCCACATCCT AATGATCAAC AGTTACAAAA TGAAAGAACG 
GCCATGTTTC CTCTCACCAG ATTACCTGCA CTACACAGTT CGGTTAATGA AATTCTAGCA 
AAACACAAGT TTCACAACAG TTTAGAGATG CAAAATGAGC ATCTCAGGCA AATCAAAGTC 
ATGCAGAATT GCAATGGGAC AAGTAAAGAT GATTCTTTCA TTAAGTCTAC AAAAGAATTT 
CAATCCAATC CTTTTTTAAA ATCAGGCTGG TTAGAACTTC GCCCTAATCA GCGCCTAAAA 
GAAGGATCTT CTGAAACTTC TTGTGAAAAT TTACTGAACT TACTTCTCCA GTCCCAGACT 
AATAACAAGA AAAAGCTAAG ACAATACCCT GGAAAACAAG GTTTAGCAAA TAGAAAGTGT 
AACAGTCAAG ATAGTGAAGA ACATCTGCAG CCAAATATTT CTGAGCAGCA GCCAAATTTC 
CCAAAGCACT TATCACAAGA TTCTCAATAC CAGCCATACA GTCAAAAAAC TAATAATGGT 
CTGATGTGGC CTGATCTTGA ACAAGCTTCT GAGGGCACTC AGTTAGGTGA TGTCCAACAT 
TTTTTGCAAG CACAGCCAAG TGAATATATT GAAGAGCTGT TAAAACAACA TTTTGATCAG 
TCACTCCGTA ACTGTAACCA AAATAGGGAC AGTCCTCTTA ACCACTTTAA ACTTGAAGAA 
GGTGCTCAGG CAGCTAATCA GTATCAAAAA CACCATCCAT ATCCTCAGCA ATTTGAGATG 
GAACCTAAGC AAAGTTTAAG CAACATGAAT TCCCCCTTCA GTCAAATTCA AGAAAATAAT 
ACAGTTAACC CTAACCATTC CTATCCTGAC AAACAAATAT ATCTGTCACA AAAAACAGAA 
AATAGCTGTG GTTTTGCAGA GAACAAACCT GACCCTTTGC AATACATGCA GTATTATCAA 
GCAAATAGTA CTGAGCAACA AGAAATAAGC AATTATGAGC ACGATCTTTC GGCCCGAATA 
GCAAAGCAGA CACATTGCCT GCCAGAGAGC TTTAGTTCAT CCCCACATTT GCATGATAAA 
ATGGGATATT ATTCTCAATT TGAAAACCAG GTTGACTGTA TTCCAAAGCA TGCTGCTCTT 
AGGAATCACT TGCTGCAACG AAGTAGATCA GTTAAAGCTG ATAGATTGGT TAAAGCTGAG 
AAGAGTTCCC ACTCTAATGC CTGCATGCAT ACACCTATGG AAAGCAAAAC TAAAAAAAAC 
ACAGATAAAA AAATCAAACA AGAAACTCGC CATTCAAGCA CAGAGGCTGG ACAACAAAAG 
AGTATTATTG AAACAATGGA ACAGCAGATG AGACATTATC CTGGTAAATC ATCGTTTCAT 
CGTCAAGTAT CAAGTATCAA ATCTCCCAAG CATGTGAAGG TGGAGTCTTC AGGGCCTGTC 
ACAGTCCTAA CTACACACAC TAATTTTTCT ACACTAGACC AACAAACTGT TCCTCCGCAT 
GAGAAAACAC CAACCAAAAG AGCAGCAGGT TCTGCTCTAC ATAACTTTAT AGAATCACCC 
TCAAATCTAC TCAGTACACC AGTAAAAAAT CTTTTGGACA CACCTATAAA AACGCAGTAT 
GATTTTCCAT CATGCAGTTG TGTAGATCAA ATAATTGAAA AAGATGAAGG ACCTTATTAT 
ACACATCTTG GTGCTGGACC CAATGTGGCA GCTATCAGAG AAATGATGGA AGAAAGGTTT 
GGCCAAAAGG GGAATGCCAT CAGAATTGAG AGGGTTGTTT ATACAGGAAA AGAAGGCAAA 
AGTGCTCAGG GATGTCCTAT TGCTAAATGG GTGATTCGGC GTAGTGGGAC AGATGAGAAA 
ATGCTGTGTT TGGTGAGGGA ACGTGCTGGT CACTCCTGCG AAACAGCAGT GATTGTCATT 
CTGATCTTGG TTTGGGAAGG CATTTCATTC TCTTTGGCAG ACAGGCTTTA CTCAGAGCTC 
ACAGAAACGC TAAATAAGTA TGGAACCTTA ACAAACCGAC GCTGTGCACG TAACGAAGAA 
CGTACCTGTG CATGCCAGGG TCTGGACTCT GAAACCTGCG GTGCCTCCTT TTCCTTTGGG 
TGCTCATGGA GTATGTATTA TAACGGGTGT AAATTCGCTA GAAGTAAAGT ACCAAGAAAA 
TTCAAACTCC TTGGTGATGA TCCAAAATCG GAAGAAAAGT TGGAAGCTAA TTTACAGCAC 
TTATCAACAC TGATGGCTCC AATTTACAAG AAGCTTGCCC CTGATGCCTA TCATAACCAG 
ATTGAGCATG AGCACAGAGC TCCAGATTGC CGTCTTGGTT TGAAGGAAGG TCGACCATTT 
TCTGGGGTTA CTGCTTGCTT GGATTTTTGC GCTCATTCCC ACCGGGACTT GCACAACATG 
CAAAATGGCA GCACACTGGT TTGCACTTTA ACCAGAGAAG ACAATCGTGA AAATGGAAAA 
ATTCCACAAG ATGAGCAACT GCATGTCTTA CCCCTTTATA AAGTATCCAA TGTGGATGAG 
TTTGGCAGTT CTGAGAGTCA GGAAGAGAAG AAAAGAACTG GTGCTATTCA AGTTCTCACA 
TCTTTCCGAA GGCAAGTGCG AATGCTAGCT GAGCCTGTAA AAACTTGCCG GCAAAAGAAG 
TTGGAAAAAC AAGAAGCCAA GAAAGCAGCA GCAGAGAAGT TAAATGCCGC TTTAGAAAAT 
GGTCACAGCA AGAACGAAAA GGAGAAGTCC TCACGGCATA AAAACGCACA TGTAGAAGCT 
TCTACGCAGG ACAAACAGCA AGCAGAATTT TCTCGAATGC CAGCCTTGCA ACAAAACACC 
CTTCTCAATA TGAACCATAG GAACTCTTTA AACCATTATC CATCATCCGC CCCTACAAAT 
CCTTACACAC GAATTCCTAA TCTGCCTGAC CCATATCAGA ATAGTTTTTC TCCCTACCCT 
GGATCAAGCC ACATGAATAT TTTTCAAACC GCTGCAAGCT CATCAGTATC CTGTGCAAAT 
AGCCCTATGA ATTCTTTTCA AGGATCCTTT AATCAAGGTA ACTCTTGCCC CTCTTATCCC 
AGTAATGGAA ATGCCTCCTT GCACAACTGT CCCCCTTATC TTGACTCTTC ACAGCAACAA 
TCCATGGATT ATTACAAGTT TAAAAACCAA GATTCAATGG CTAAATTAGG TGTGCCCCCA 
ATTCAATCAT TGTACCAGCA GGGGTTTGCT AATAATCAGG CTTATGGTAA CAAAATGTTT 
AGTTATGGAA ACCAAAGAGT GCAAGCTGAT GATTTCAGTG GCTGTAATGC TTATCCAGCA 
CCTCAGGCTG ATATAATGAA TCCAGCCACA AGTTCACGGC CTATTACCAG TAATCAAGAC 
TTCTCTTCTG CGACTAAGAA TGGTGAATAT CCTCTTCCAC ACTCCTTCCA GGGTCATGAA 
AGCAACATGT CATCAAGTAT ATTGGGAGGC AGAGCTGGTT CTCTGCCATT CCCTGGTAAA 
CAGAGTGAAA TGCATTCACG CACAGCAAAT GGAACATCGG AAATGCTTCC AGGTCTTAAC 
CATGATAGAA CTAATTTTGG AGGCGCAGGC AGAACTAATG GTGCTAATGT TCAGGGAAAG 
CTACCGTACA CCTTACCTCC TGTACCAGAA GAGCCTGCAG AAGTTTGGTC AGACAGTGAA 
CAGAATTTTC TTGATCCTGA TATAGGAGGT GTAGCAGTTT GCCCAACTCA TGGGTCAATC 
TTGATTGAAT GTGCAAAGCG TGAGCTCCAC GCAACAACAC CTTTAAAGAA TCCCAACAGA 
AATCACCCTA CCAGAATATC TTTGGTGTTC TACCAACACA AGAGCATGAA CGAGCCAAAG 
CATGGCCTGG CATTGTGGGA AGCAAAAATG GCAGGCAAAG CCAGGGAAAA GGAAGAAGAT 
TGTGAAAGGT ATGGTCCAGA TTATGTGGCT CCTAAATCTT ACAACAGGAA AGCAAAGCGG 
GAGCCTGTGG AGCCAATACA TGAACCTGCT GAACCAACTT ATCTTCGTTT CATCAAGTCA 
CTTACGCAGA GGACGATGTC AATAACCACA GACTCTCAAG TCACTACATC TCCATATGCC 
TTAACACGGG TTACAGGTCC TTATAACAGA TATATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet2 ( XM_012955515.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810969.1 Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X2, mRNA.

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CloneID	OXa33117
Clone ID Related Accession (Same CDS sequence)	XM_012955515.2 , XM_018096256.1
Accession Version	XM_012955515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	methylcytosine dioxygenase TET2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG ACAGAACCAA CCATGACGAG GGCAATAGAT TGAGTCCAAC CCTATTATAT 
TCTTCGTCAG CTTGCCAAAA CAAGAACGGT CTATTACAAA ATGGGAACCT GCCAACAGAC 
AAAGACCTTC TAGTTAATGG GGACATATGT TTACCAACCA AAAACAACTA TAATGTTGCT 
TACATGAAGG GAAGTCAAGA ACATGGTGCT TCTGCTCTCA TCCATGACAA CAAAGCTTAT 
CCCAACCACA TACAAAATGG TGGAATAAAG CGCACATATA GTGAACCGTT GTTTTCTGAA 
TCCCAGTTGA AAAAGGTCAG AACAGATGAG ATTAATGGAG AAAATAAGGG TGACCAAAGG 
CAAAATAATA TTCAAGCAAG TGTCTCTGAG TTCTGCAATG AAGATCAGTC AGTAAGAATT 
TTCCAACCAG TTAATTCACC AGCTCTTCAG GTTCCAAATG AGCAGAAGAA AGACCTTAAT 
TACAGCAACA GAGACATTGT CTCATGTCTT AAAGACAAGA CAGTGCCAAT GTGTAATGGT 
GCTACTGCTT CTGTATCTTC CATGGAAAAC GTGAATAATG AGCTCCTGGA AAAACTGCCT 
TCTCAATATT ATCCTGAACA TGTTTCTACT GTGGTGCATA ATAACACATC TCTTGCAAAT 
GCCATTAATA CGCTAAATGA TGCTAGCAAT GATTTGTCTA CTCTGGCAAC TCATCCGTCA 
CATACCTCAG GGCAGATGAC TCCTCAACAA ACTTCGGTGC TGCCTCAAGT GTCAGGTGCA 
ATTGTTGATG GGGCCCCCAA TAGTGACCAT GGTAGTGAGC CACTTGTATT ACCAGGAAGC 
TATTCACTTA ATAGACAAGA ACAGAAGCCA CTTTCACAGG AAACTCTTAA TTATAATTCC 
CATAATTTAG TTGGAAGCAT GGGTGATGCA TGCAAATCCA TGGGCCAGAT GTCAGCACAA 
TGTCTAGATG TTTCTGGTAA TGGTTCATTT CATATCCAAA ATAGCTCATC AAATGAGATA 
GCTTCTGAAT TAGAGGCCAT CAAGAAAGAT CCAGCACCAT CACTGCAATT ACATCATATT 
ATGCCCAACA AGCAAAATGA GTCCAGTATA AGTCAATCAC ATGATGGGCA TCCAATTGCA 
TCACAAACTA ATGAATCTTG CCATTCACAT GATCCAAGAG TATCTCCACA TCCTAATGAT 
CAACAGTTAC AAAATGAAAG AACGGCCATG TTTCCTCTCA CCAGATTACC TGCACTACAC 
AGTTCGGTTA ATGAAATTCT AGCAAAACAC AAGTTTCACA ACAGTTTAGA GATGCAAAAT 
GAGCATCTCA GGCAAATCAA AGTCATGCAG AATTGCAATG GGACAAGTAA AGATGATTCT 
TTCATTAAGT CTACAAAAGA ATTTCAATCC AATCCTTTTT TAAAATCAGG CTGGTTAGAA 
CTTCGCCCTA ATCAGCGCCT AAAAGAAGGA TCTTCTGAAA CTTCTTGTGA AAATTTACTG 
AACTTACTTC TCCAGTCCCA GACTAATAAC AAGAAAAAGC TAAGACAATA CCCTGGAAAA 
CAAGGTTTAG CAAATAGAAA GTGTAACAGT CAAGATAGTG AAGAACATCT GCAGCCAAAT 
ATTTCTGAGC AGCAGCCAAA TTTCCCAAAG CACTTATCAC AAGATTCTCA ATACCAGCCA 
TACAGTCAAA AAACTAATAA TGGTCTGATG TGGCCTGATC TTGAACAAGC TTCTGAGGGC 
ACTCAGTTAG GTGATGTCCA ACATTTTTTG CAAGCACAGC CAAGTGAATA TATTGAAGAG 
CTGTTAAAAC AACATTTTGA TCAGTCACTC CGTAACTGTA ACCAAAATAG GGACAGTCCT 
CTTAACCACT TTAAACTTGA AGAAGGTGCT CAGGCAGCTA ATCAGTATCA AAAACACCAT 
CCATATCCTC AGCAATTTGA GATGGAACCT AAGCAAAGTT TAAGCAACAT GAATTCCCCC 
TTCAGTCAAA TTCAAGAAAA TAATACAGTT AACCCTAACC ATTCCTATCC TGACAAACAA 
ATATATCTGT CACAAAAAAC AGAAAATAGC TGTGGTTTTG CAGAGAACAA ACCTGACCCT 
TTGCAATACA TGCAGTATTA TCAAGCAAAT AGTACTGAGC AACAAGAAAT AAGCAATTAT 
GAGCACGATC TTTCGGCCCG AATAGCAAAG CAGACACATT GCCTGCCAGA GAGCTTTAGT 
TCATCCCCAC ATTTGCATGA TAAAATGGGA TATTATTCTC AATTTGAAAA CCAGGTTGAC 
TGTATTCCAA AGCATGCTGC TCTTAGGAAT CACTTGCTGC AACGAAGTAG ATCAGTTAAA 
GCTGATAGAT TGGTTAAAGC TGAGAAGAGT TCCCACTCTA ATGCCTGCAT GCATACACCT 
ATGGAAAGCA AAACTAAAAA AAACACAGAT AAAAAAATCA AACAAGAAAC TCGCCATTCA 
AGCACAGAGG CTGGACAACA AAAGAGTATT ATTGAAACAA TGGAACAGCA GATGAGACAT 
TATCCTGGTA AATCATCGTT TCATCGTCAA GTATCAAGTA TCAAATCTCC CAAGCATGTG 
AAGGTGGAGT CTTCAGGGCC TGTCACAGTC CTAACTACAC ACACTAATTT TTCTACACTA 
GACCAACAAA CTGTTCCTCC GCATGAGAAA ACACCAACCA AAAGAGCAGC AGGTTCTGCT 
CTACATAACT TTATAGAATC ACCCTCAAAT CTACTCAGTA CACCAGTAAA AAATCTTTTG 
GACACACCTA TAAAAACGCA GTATGATTTT CCATCATGCA GTTGTGTAGA TCAAATAATT 
GAAAAAGATG AAGGACCTTA TTATACACAT CTTGGTGCTG GACCCAATGT GGCAGCTATC 
AGAGAAATGA TGGAAGAAAG GTTTGGCCAA AAGGGGAATG CCATCAGAAT TGAGAGGGTT 
GTTTATACAG GAAAAGAAGG CAAAAGTGCT CAGGGATGTC CTATTGCTAA ATGGGTGATT 
CGGCGTAGTG GGACAGATGA GAAAATGCTG TGTTTGGTGA GGGAACGTGC TGGTCACTCC 
TGCGAAACAG CAGTGATTGT CATTCTGATC TTGGTTTGGG AAGGCATTTC ATTCTCTTTG 
GCAGACAGGC TTTACTCAGA GCTCACAGAA ACGCTAAATA AGTATGGAAC CTTAACAAAC 
CGACGCTGTG CACGTAACGA AGAACGTACC TGTGCATGCC AGGGTCTGGA CTCTGAAACC 
TGCGGTGCCT CCTTTTCCTT TGGGTGCTCA TGGAGTATGT ATTATAACGG GTGTAAATTC 
GCTAGAAGTA AAGTACCAAG AAAATTCAAA CTCCTTGGTG ATGATCCAAA ATCGGAAGAA 
AAGTTGGAAG CTAATTTACA GCACTTATCA ACACTGATGG CTCCAATTTA CAAGAAGCTT 
GCCCCTGATG CCTATCATAA CCAGATTGAG CATGAGCACA GAGCTCCAGA TTGCCGTCTT 
GGTTTGAAGG AAGGTCGACC ATTTTCTGGG GTTACTGCTT GCTTGGATTT TTGCGCTCAT 
TCCCACCGGG ACTTGCACAA CATGCAAAAT GGCAGCACAC TGGTTTGCAC TTTAACCAGA 
GAAGACAATC GTGAAAATGG AAAAATTCCA CAAGATGAGC AACTGCATGT CTTACCCCTT 
TATAAAGTAT CCAATGTGGA TGAGTTTGGC AGTTCTGAGA GTCAGGAAGA GAAGAAAAGA 
ACTGGTGCTA TTCAAGTTCT CACATCTTTC CGAAGGCAAG TGCGAATGCT AGCTGAGCCT 
GTAAAAACTT GCCGGCAAAA GAAGTTGGAA AAACAAGAAG CCAAGAAAGC AGCAGCAGAG 
AAGTTAAATG CCGCTTTAGA AAATGGTCAC AGCAAGAACG AAAAGGAGAA GTCCTCACGG 
CATAAAAACG CACATGTAGA AGCTTCTACG CAGGACAAAC AGCAAGCAGA ATTTTCTCGA 
ATGCCAGCCT TGCAACAAAA CACCCTTCTC AATATGAACC ATAGGAACTC TTTAAACCAT 
TATCCATCAT CCGCCCCTAC AAATCCTTAC ACACGAATTC CTAATCTGCC TGACCCATAT 
CAGAATAGTT TTTCTCCCTA CCCTGGATCA AGCCACATGA ATATTTTTCA AACCGCTGCA 
AGCTCATCAG TATCCTGTGC AAATAGCCCT ATGAATTCTT TTCAAGGATC CTTTAATCAA 
GGTAACTCTT GCCCCTCTTA TCCCAGTAAT GGAAATGCCT CCTTGCACAA CTGTCCCCCT 
TATCTTGACT CTTCACAGCA ACAATCCATG GATTATTACA AGTTTAAAAA CCAAGATTCA 
ATGGCTAAAT TAGGTGTGCC CCCAATTCAA TCATTGTACC AGCAGGGGTT TGCTAATAAT 
CAGGCTTATG GTAACAAAAT GTTTAGTTAT GGAAACCAAA GAGTGCAAGC TGATGATTTC 
AGTGGCTGTA ATGCTTATCC AGCACCTCAG GCTGATATAA TGAATCCAGC CACAAGTTCA 
CGGCCTATTA CCAGTAATCA AGACTTCTCT TCTGCGACTA AGAATGGTGA ATATCCTCTT 
CCACACTCCT TCCAGGGTCA TGAAAGCAAC ATGTCATCAA GTATATTGGG AGGCAGAGCT 
GGTTCTCTGC CATTCCCTGG TAAACAGAGT GAAATGCATT CACGCACAGC AAATGGAACA 
TCGGAAATGC TTCCAGGTCT TAACCATGAT AGAACTAATT TTGGAGGCGC AGGCAGAACT 
AATGGTGCTA ATGTTCAGGG AAAGCTACCG TACACCTTAC CTCCTGTACC AGAAGAGCCT 
GCAGAAGTTT GGTCAGACAG TGAACAGAAT TTTCTTGATC CTGATATAGG AGGTGTAGCA 
GTTTGCCCAA CTCATGGGTC AATCTTGATT GAATGTGCAA AGCGTGAGCT CCACGCAACA 
ACACCTTTAA AGAATCCCAA CAGAAATCAC CCTACCAGAA TATCTTTGGT GTTCTACCAA 
CACAAGAGCA TGAACGAGCC AAAGCATGGC CTGGCATTGT GGGAAGCAAA AATGGCAGGC 
AAAGCCAGGG AAAAGGAAGA AGATTGTGAA AGGTATGGTC CAGATTATGT GGCTCCTAAA 
TCTTACAACA GGAAAGCAAA GCGGGAGCCT GTGGAGCCAA TACATGAACC TGCTGAACCA 
ACTTATCTTC GTTTCATCAA GTCACTTACG CAGAGGACGA TGTCAATAAC CACAGACTCT 
CAAGTCACTA CATCTCCATA TGCCTTAACA CGGGTTACAG GTCCTTATAA CAGATATATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810969.1
CDS	1097..6379
Translation	MEEDRTNHDEGNRLSPTLLYSSSACQNKNGLLQNGNLPTDKDLLVNGDICLPTKNNYNVAYMKGSQEHGASALIHDNKAYPNHIQNGGIKRTYSEPLFSESQLKKVRTDEINGENKGDQRQNNIQASVSEFCNEDQSVRIFQPVNSPALQVPNEQKKDLNYSNRDIVSCLKDKTVPMCNGATASVSSMENVNNELLEKLPSQYYPEHVSTVVHNNTSLANAINTLNDASNDLSTLATHPSHTSGQMTPQQTSVLPQVSGAIVDGAPNSDHGSEPLVLPGSYSLNRQEQKPLSQETLNYNSHNLVGSMGDACKSMGQMSAQCLDVSGNGSFHIQNSSSNEIASELEAIKKDPAPSLQLHHIMPNKQNESSISQSHDGHPIASQTNESCHSHDPRVSPHPNDQQLQNERTAMFPLTRLPALHSSVNEILAKHKFHNSLEMQNEHLRQIKVMQNCNGTSKDDSFIKSTKEFQSNPFLKSGWLELRPNQRLKEGSSETSCENLLNLLLQSQTNNKKKLRQYPGKQGLANRKCNSQDSEEHLQPNISEQQPNFPKHLSQDSQYQPYSQKTNNGLMWPDLEQASEGTQLGDVQHFLQAQPSEYIEELLKQHFDQSLRNCNQNRDSPLNHFKLEEGAQAANQYQKHHPYPQQFEMEPKQSLSNMNSPFSQIQENNTVNPNHSYPDKQIYLSQKTENSCGFAENKPDPLQYMQYYQANSTEQQEISNYEHDLSARIAKQTHCLPESFSSSPHLHDKMGYYSQFENQVDCIPKHAALRNHLLQRSRSVKADRLVKAEKSSHSNACMHTPMESKTKKNTDKKIKQETRHSSTEAGQQKSIIETMEQQMRHYPGKSSFHRQVSSIKSPKHVKVESSGPVTVLTTHTNFSTLDQQTVPPHEKTPTKRAAGSALHNFIESPSNLLSTPVKNLLDTPIKTQYDFPSCSCVDQIIEKDEGPYYTHLGAGPNVAAIREMMEERFGQKGNAIRIERVVYTGKEGKSAQGCPIAKWVIRRSGTDEKMLCLVRERAGHSCETAVIVILILVWEGISFSLADRLYSELTETLNKYGTLTNRRCARNEERTCACQGLDSETCGASFSFGCSWSMYYNGCKFARSKVPRKFKLLGDDPKSEEKLEANLQHLSTLMAPIYKKLAPDAYHNQIEHEHRAPDCRLGLKEGRPFSGVTACLDFCAHSHRDLHNMQNGSTLVCTLTREDNRENGKIPQDEQLHVLPLYKVSNVDEFGSSESQEEKKRTGAIQVLTSFRRQVRMLAEPVKTCRQKKLEKQEAKKAAAEKLNAALENGHSKNEKEKSSRHKNAHVEASTQDKQQAEFSRMPALQQNTLLNMNHRNSLNHYPSSAPTNPYTRIPNLPDPYQNSFSPYPGSSHMNIFQTAASSSVSCANSPMNSFQGSFNQGNSCPSYPSNGNASLHNCPPYLDSSQQQSMDYYKFKNQDSMAKLGVPPIQSLYQQGFANNQAYGNKMFSYGNQRVQADDFSGCNAYPAPQADIMNPATSSRPITSNQDFSSATKNGEYPLPHSFQGHESNMSSSILGGRAGSLPFPGKQSEMHSRTANGTSEMLPGLNHDRTNFGGAGRTNGANVQGKLPYTLPPVPEEPAEVWSDSEQNFLDPDIGGVAVCPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAGKAREKEEDCERYGPDYVAPKSYNRKAKREPVEPIHEPAEPTYLRFIKSLTQRTMSITTDSQVTTSPYALTRVTGPYNRYI

Target ORF information:

RefSeq Version	XM_012955515.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955515.2

ORF Insert Sequence:

ATGGAAGAGG ACAGAACCAA CCATGACGAG GGCAATAGAT TGAGTCCAAC CCTATTATAT 
TCTTCGTCAG CTTGCCAAAA CAAGAACGGT CTATTACAAA ATGGGAACCT GCCAACAGAC 
AAAGACCTTC TAGTTAATGG GGACATATGT TTACCAACCA AAAACAACTA TAATGTTGCT 
TACATGAAGG GAAGTCAAGA ACATGGTGCT TCTGCTCTCA TCCATGACAA CAAAGCTTAT 
CCCAACCACA TACAAAATGG TGGAATAAAG CGCACATATA GTGAACCGTT GTTTTCTGAA 
TCCCAGTTGA AAAAGGTCAG AACAGATGAG ATTAATGGAG AAAATAAGGG TGACCAAAGG 
CAAAATAATA TTCAAGCAAG TGTCTCTGAG TTCTGCAATG AAGATCAGTC AGTAAGAATT 
TTCCAACCAG TTAATTCACC AGCTCTTCAG GTTCCAAATG AGCAGAAGAA AGACCTTAAT 
TACAGCAACA GAGACATTGT CTCATGTCTT AAAGACAAGA CAGTGCCAAT GTGTAATGGT 
GCTACTGCTT CTGTATCTTC CATGGAAAAC GTGAATAATG AGCTCCTGGA AAAACTGCCT 
TCTCAATATT ATCCTGAACA TGTTTCTACT GTGGTGCATA ATAACACATC TCTTGCAAAT 
GCCATTAATA CGCTAAATGA TGCTAGCAAT GATTTGTCTA CTCTGGCAAC TCATCCGTCA 
CATACCTCAG GGCAGATGAC TCCTCAACAA ACTTCGGTGC TGCCTCAAGT GTCAGGTGCA 
ATTGTTGATG GGGCCCCCAA TAGTGACCAT GGTAGTGAGC CACTTGTATT ACCAGGAAGC 
TATTCACTTA ATAGACAAGA ACAGAAGCCA CTTTCACAGG AAACTCTTAA TTATAATTCC 
CATAATTTAG TTGGAAGCAT GGGTGATGCA TGCAAATCCA TGGGCCAGAT GTCAGCACAA 
TGTCTAGATG TTTCTGGTAA TGGTTCATTT CATATCCAAA ATAGCTCATC AAATGAGATA 
GCTTCTGAAT TAGAGGCCAT CAAGAAAGAT CCAGCACCAT CACTGCAATT ACATCATATT 
ATGCCCAACA AGCAAAATGA GTCCAGTATA AGTCAATCAC ATGATGGGCA TCCAATTGCA 
TCACAAACTA ATGAATCTTG CCATTCACAT GATCCAAGAG TATCTCCACA TCCTAATGAT 
CAACAGTTAC AAAATGAAAG AACGGCCATG TTTCCTCTCA CCAGATTACC TGCACTACAC 
AGTTCGGTTA ATGAAATTCT AGCAAAACAC AAGTTTCACA ACAGTTTAGA GATGCAAAAT 
GAGCATCTCA GGCAAATCAA AGTCATGCAG AATTGCAATG GGACAAGTAA AGATGATTCT 
TTCATTAAGT CTACAAAAGA ATTTCAATCC AATCCTTTTT TAAAATCAGG CTGGTTAGAA 
CTTCGCCCTA ATCAGCGCCT AAAAGAAGGA TCTTCTGAAA CTTCTTGTGA AAATTTACTG 
AACTTACTTC TCCAGTCCCA GACTAATAAC AAGAAAAAGC TAAGACAATA CCCTGGAAAA 
CAAGGTTTAG CAAATAGAAA GTGTAACAGT CAAGATAGTG AAGAACATCT GCAGCCAAAT 
ATTTCTGAGC AGCAGCCAAA TTTCCCAAAG CACTTATCAC AAGATTCTCA ATACCAGCCA 
TACAGTCAAA AAACTAATAA TGGTCTGATG TGGCCTGATC TTGAACAAGC TTCTGAGGGC 
ACTCAGTTAG GTGATGTCCA ACATTTTTTG CAAGCACAGC CAAGTGAATA TATTGAAGAG 
CTGTTAAAAC AACATTTTGA TCAGTCACTC CGTAACTGTA ACCAAAATAG GGACAGTCCT 
CTTAACCACT TTAAACTTGA AGAAGGTGCT CAGGCAGCTA ATCAGTATCA AAAACACCAT 
CCATATCCTC AGCAATTTGA GATGGAACCT AAGCAAAGTT TAAGCAACAT GAATTCCCCC 
TTCAGTCAAA TTCAAGAAAA TAATACAGTT AACCCTAACC ATTCCTATCC TGACAAACAA 
ATATATCTGT CACAAAAAAC AGAAAATAGC TGTGGTTTTG CAGAGAACAA ACCTGACCCT 
TTGCAATACA TGCAGTATTA TCAAGCAAAT AGTACTGAGC AACAAGAAAT AAGCAATTAT 
GAGCACGATC TTTCGGCCCG AATAGCAAAG CAGACACATT GCCTGCCAGA GAGCTTTAGT 
TCATCCCCAC ATTTGCATGA TAAAATGGGA TATTATTCTC AATTTGAAAA CCAGGTTGAC 
TGTATTCCAA AGCATGCTGC TCTTAGGAAT CACTTGCTGC AACGAAGTAG ATCAGTTAAA 
GCTGATAGAT TGGTTAAAGC TGAGAAGAGT TCCCACTCTA ATGCCTGCAT GCATACACCT 
ATGGAAAGCA AAACTAAAAA AAACACAGAT AAAAAAATCA AACAAGAAAC TCGCCATTCA 
AGCACAGAGG CTGGACAACA AAAGAGTATT ATTGAAACAA TGGAACAGCA GATGAGACAT 
TATCCTGGTA AATCATCGTT TCATCGTCAA GTATCAAGTA TCAAATCTCC CAAGCATGTG 
AAGGTGGAGT CTTCAGGGCC TGTCACAGTC CTAACTACAC ACACTAATTT TTCTACACTA 
GACCAACAAA CTGTTCCTCC GCATGAGAAA ACACCAACCA AAAGAGCAGC AGGTTCTGCT 
CTACATAACT TTATAGAATC ACCCTCAAAT CTACTCAGTA CACCAGTAAA AAATCTTTTG 
GACACACCTA TAAAAACGCA GTATGATTTT CCATCATGCA GTTGTGTAGA TCAAATAATT 
GAAAAAGATG AAGGACCTTA TTATACACAT CTTGGTGCTG GACCCAATGT GGCAGCTATC 
AGAGAAATGA TGGAAGAAAG GTTTGGCCAA AAGGGGAATG CCATCAGAAT TGAGAGGGTT 
GTTTATACAG GAAAAGAAGG CAAAAGTGCT CAGGGATGTC CTATTGCTAA ATGGGTGATT 
CGGCGTAGTG GGACAGATGA GAAAATGCTG TGTTTGGTGA GGGAACGTGC TGGTCACTCC 
TGCGAAACAG CAGTGATTGT CATTCTGATC TTGGTTTGGG AAGGCATTTC ATTCTCTTTG 
GCAGACAGGC TTTACTCAGA GCTCACAGAA ACGCTAAATA AGTATGGAAC CTTAACAAAC 
CGACGCTGTG CACGTAACGA AGAACGTACC TGTGCATGCC AGGGTCTGGA CTCTGAAACC 
TGCGGTGCCT CCTTTTCCTT TGGGTGCTCA TGGAGTATGT ATTATAACGG GTGTAAATTC 
GCTAGAAGTA AAGTACCAAG AAAATTCAAA CTCCTTGGTG ATGATCCAAA ATCGGAAGAA 
AAGTTGGAAG CTAATTTACA GCACTTATCA ACACTGATGG CTCCAATTTA CAAGAAGCTT 
GCCCCTGATG CCTATCATAA CCAGATTGAG CATGAGCACA GAGCTCCAGA TTGCCGTCTT 
GGTTTGAAGG AAGGTCGACC ATTTTCTGGG GTTACTGCTT GCTTGGATTT TTGCGCTCAT 
TCCCACCGGG ACTTGCACAA CATGCAAAAT GGCAGCACAC TGGTTTGCAC TTTAACCAGA 
GAAGACAATC GTGAAAATGG AAAAATTCCA CAAGATGAGC AACTGCATGT CTTACCCCTT 
TATAAAGTAT CCAATGTGGA TGAGTTTGGC AGTTCTGAGA GTCAGGAAGA GAAGAAAAGA 
ACTGGTGCTA TTCAAGTTCT CACATCTTTC CGAAGGCAAG TGCGAATGCT AGCTGAGCCT 
GTAAAAACTT GCCGGCAAAA GAAGTTGGAA AAACAAGAAG CCAAGAAAGC AGCAGCAGAG 
AAGTTAAATG CCGCTTTAGA AAATGGTCAC AGCAAGAACG AAAAGGAGAA GTCCTCACGG 
CATAAAAACG CACATGTAGA AGCTTCTACG CAGGACAAAC AGCAAGCAGA ATTTTCTCGA 
ATGCCAGCCT TGCAACAAAA CACCCTTCTC AATATGAACC ATAGGAACTC TTTAAACCAT 
TATCCATCAT CCGCCCCTAC AAATCCTTAC ACACGAATTC CTAATCTGCC TGACCCATAT 
CAGAATAGTT TTTCTCCCTA CCCTGGATCA AGCCACATGA ATATTTTTCA AACCGCTGCA 
AGCTCATCAG TATCCTGTGC AAATAGCCCT ATGAATTCTT TTCAAGGATC CTTTAATCAA 
GGTAACTCTT GCCCCTCTTA TCCCAGTAAT GGAAATGCCT CCTTGCACAA CTGTCCCCCT 
TATCTTGACT CTTCACAGCA ACAATCCATG GATTATTACA AGTTTAAAAA CCAAGATTCA 
ATGGCTAAAT TAGGTGTGCC CCCAATTCAA TCATTGTACC AGCAGGGGTT TGCTAATAAT 
CAGGCTTATG GTAACAAAAT GTTTAGTTAT GGAAACCAAA GAGTGCAAGC TGATGATTTC 
AGTGGCTGTA ATGCTTATCC AGCACCTCAG GCTGATATAA TGAATCCAGC CACAAGTTCA 
CGGCCTATTA CCAGTAATCA AGACTTCTCT TCTGCGACTA AGAATGGTGA ATATCCTCTT 
CCACACTCCT TCCAGGGTCA TGAAAGCAAC ATGTCATCAA GTATATTGGG AGGCAGAGCT 
GGTTCTCTGC CATTCCCTGG TAAACAGAGT GAAATGCATT CACGCACAGC AAATGGAACA 
TCGGAAATGC TTCCAGGTCT TAACCATGAT AGAACTAATT TTGGAGGCGC AGGCAGAACT 
AATGGTGCTA ATGTTCAGGG AAAGCTACCG TACACCTTAC CTCCTGTACC AGAAGAGCCT 
GCAGAAGTTT GGTCAGACAG TGAACAGAAT TTTCTTGATC CTGATATAGG AGGTGTAGCA 
GTTTGCCCAA CTCATGGGTC AATCTTGATT GAATGTGCAA AGCGTGAGCT CCACGCAACA 
ACACCTTTAA AGAATCCCAA CAGAAATCAC CCTACCAGAA TATCTTTGGT GTTCTACCAA 
CACAAGAGCA TGAACGAGCC AAAGCATGGC CTGGCATTGT GGGAAGCAAA AATGGCAGGC 
AAAGCCAGGG AAAAGGAAGA AGATTGTGAA AGGTATGGTC CAGATTATGT GGCTCCTAAA 
TCTTACAACA GGAAAGCAAA GCGGGAGCCT GTGGAGCCAA TACATGAACC TGCTGAACCA 
ACTTATCTTC GTTTCATCAA GTCACTTACG CAGAGGACGA TGTCAATAAC CACAGACTCT 
CAAGTCACTA CATCTCCATA TGCCTTAACA CGGGTTACAG GTCCTTATAA CAGATATATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet2 ( XM_018096256.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951745.1 Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X3, mRNA.

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CloneID	OXa33117
Clone ID Related Accession (Same CDS sequence)	XM_012955515.2 , XM_018096256.1
Accession Version	XM_018096256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	methylcytosine dioxygenase TET2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG ACAGAACCAA CCATGACGAG GGCAATAGAT TGAGTCCAAC CCTATTATAT 
TCTTCGTCAG CTTGCCAAAA CAAGAACGGT CTATTACAAA ATGGGAACCT GCCAACAGAC 
AAAGACCTTC TAGTTAATGG GGACATATGT TTACCAACCA AAAACAACTA TAATGTTGCT 
TACATGAAGG GAAGTCAAGA ACATGGTGCT TCTGCTCTCA TCCATGACAA CAAAGCTTAT 
CCCAACCACA TACAAAATGG TGGAATAAAG CGCACATATA GTGAACCGTT GTTTTCTGAA 
TCCCAGTTGA AAAAGGTCAG AACAGATGAG ATTAATGGAG AAAATAAGGG TGACCAAAGG 
CAAAATAATA TTCAAGCAAG TGTCTCTGAG TTCTGCAATG AAGATCAGTC AGTAAGAATT 
TTCCAACCAG TTAATTCACC AGCTCTTCAG GTTCCAAATG AGCAGAAGAA AGACCTTAAT 
TACAGCAACA GAGACATTGT CTCATGTCTT AAAGACAAGA CAGTGCCAAT GTGTAATGGT 
GCTACTGCTT CTGTATCTTC CATGGAAAAC GTGAATAATG AGCTCCTGGA AAAACTGCCT 
TCTCAATATT ATCCTGAACA TGTTTCTACT GTGGTGCATA ATAACACATC TCTTGCAAAT 
GCCATTAATA CGCTAAATGA TGCTAGCAAT GATTTGTCTA CTCTGGCAAC TCATCCGTCA 
CATACCTCAG GGCAGATGAC TCCTCAACAA ACTTCGGTGC TGCCTCAAGT GTCAGGTGCA 
ATTGTTGATG GGGCCCCCAA TAGTGACCAT GGTAGTGAGC CACTTGTATT ACCAGGAAGC 
TATTCACTTA ATAGACAAGA ACAGAAGCCA CTTTCACAGG AAACTCTTAA TTATAATTCC 
CATAATTTAG TTGGAAGCAT GGGTGATGCA TGCAAATCCA TGGGCCAGAT GTCAGCACAA 
TGTCTAGATG TTTCTGGTAA TGGTTCATTT CATATCCAAA ATAGCTCATC AAATGAGATA 
GCTTCTGAAT TAGAGGCCAT CAAGAAAGAT CCAGCACCAT CACTGCAATT ACATCATATT 
ATGCCCAACA AGCAAAATGA GTCCAGTATA AGTCAATCAC ATGATGGGCA TCCAATTGCA 
TCACAAACTA ATGAATCTTG CCATTCACAT GATCCAAGAG TATCTCCACA TCCTAATGAT 
CAACAGTTAC AAAATGAAAG AACGGCCATG TTTCCTCTCA CCAGATTACC TGCACTACAC 
AGTTCGGTTA ATGAAATTCT AGCAAAACAC AAGTTTCACA ACAGTTTAGA GATGCAAAAT 
GAGCATCTCA GGCAAATCAA AGTCATGCAG AATTGCAATG GGACAAGTAA AGATGATTCT 
TTCATTAAGT CTACAAAAGA ATTTCAATCC AATCCTTTTT TAAAATCAGG CTGGTTAGAA 
CTTCGCCCTA ATCAGCGCCT AAAAGAAGGA TCTTCTGAAA CTTCTTGTGA AAATTTACTG 
AACTTACTTC TCCAGTCCCA GACTAATAAC AAGAAAAAGC TAAGACAATA CCCTGGAAAA 
CAAGGTTTAG CAAATAGAAA GTGTAACAGT CAAGATAGTG AAGAACATCT GCAGCCAAAT 
ATTTCTGAGC AGCAGCCAAA TTTCCCAAAG CACTTATCAC AAGATTCTCA ATACCAGCCA 
TACAGTCAAA AAACTAATAA TGGTCTGATG TGGCCTGATC TTGAACAAGC TTCTGAGGGC 
ACTCAGTTAG GTGATGTCCA ACATTTTTTG CAAGCACAGC CAAGTGAATA TATTGAAGAG 
CTGTTAAAAC AACATTTTGA TCAGTCACTC CGTAACTGTA ACCAAAATAG GGACAGTCCT 
CTTAACCACT TTAAACTTGA AGAAGGTGCT CAGGCAGCTA ATCAGTATCA AAAACACCAT 
CCATATCCTC AGCAATTTGA GATGGAACCT AAGCAAAGTT TAAGCAACAT GAATTCCCCC 
TTCAGTCAAA TTCAAGAAAA TAATACAGTT AACCCTAACC ATTCCTATCC TGACAAACAA 
ATATATCTGT CACAAAAAAC AGAAAATAGC TGTGGTTTTG CAGAGAACAA ACCTGACCCT 
TTGCAATACA TGCAGTATTA TCAAGCAAAT AGTACTGAGC AACAAGAAAT AAGCAATTAT 
GAGCACGATC TTTCGGCCCG AATAGCAAAG CAGACACATT GCCTGCCAGA GAGCTTTAGT 
TCATCCCCAC ATTTGCATGA TAAAATGGGA TATTATTCTC AATTTGAAAA CCAGGTTGAC 
TGTATTCCAA AGCATGCTGC TCTTAGGAAT CACTTGCTGC AACGAAGTAG ATCAGTTAAA 
GCTGATAGAT TGGTTAAAGC TGAGAAGAGT TCCCACTCTA ATGCCTGCAT GCATACACCT 
ATGGAAAGCA AAACTAAAAA AAACACAGAT AAAAAAATCA AACAAGAAAC TCGCCATTCA 
AGCACAGAGG CTGGACAACA AAAGAGTATT ATTGAAACAA TGGAACAGCA GATGAGACAT 
TATCCTGGTA AATCATCGTT TCATCGTCAA GTATCAAGTA TCAAATCTCC CAAGCATGTG 
AAGGTGGAGT CTTCAGGGCC TGTCACAGTC CTAACTACAC ACACTAATTT TTCTACACTA 
GACCAACAAA CTGTTCCTCC GCATGAGAAA ACACCAACCA AAAGAGCAGC AGGTTCTGCT 
CTACATAACT TTATAGAATC ACCCTCAAAT CTACTCAGTA CACCAGTAAA AAATCTTTTG 
GACACACCTA TAAAAACGCA GTATGATTTT CCATCATGCA GTTGTGTAGA TCAAATAATT 
GAAAAAGATG AAGGACCTTA TTATACACAT CTTGGTGCTG GACCCAATGT GGCAGCTATC 
AGAGAAATGA TGGAAGAAAG GTTTGGCCAA AAGGGGAATG CCATCAGAAT TGAGAGGGTT 
GTTTATACAG GAAAAGAAGG CAAAAGTGCT CAGGGATGTC CTATTGCTAA ATGGGTGATT 
CGGCGTAGTG GGACAGATGA GAAAATGCTG TGTTTGGTGA GGGAACGTGC TGGTCACTCC 
TGCGAAACAG CAGTGATTGT CATTCTGATC TTGGTTTGGG AAGGCATTTC ATTCTCTTTG 
GCAGACAGGC TTTACTCAGA GCTCACAGAA ACGCTAAATA AGTATGGAAC CTTAACAAAC 
CGACGCTGTG CACGTAACGA AGAACGTACC TGTGCATGCC AGGGTCTGGA CTCTGAAACC 
TGCGGTGCCT CCTTTTCCTT TGGGTGCTCA TGGAGTATGT ATTATAACGG GTGTAAATTC 
GCTAGAAGTA AAGTACCAAG AAAATTCAAA CTCCTTGGTG ATGATCCAAA ATCGGAAGAA 
AAGTTGGAAG CTAATTTACA GCACTTATCA ACACTGATGG CTCCAATTTA CAAGAAGCTT 
GCCCCTGATG CCTATCATAA CCAGATTGAG CATGAGCACA GAGCTCCAGA TTGCCGTCTT 
GGTTTGAAGG AAGGTCGACC ATTTTCTGGG GTTACTGCTT GCTTGGATTT TTGCGCTCAT 
TCCCACCGGG ACTTGCACAA CATGCAAAAT GGCAGCACAC TGGTTTGCAC TTTAACCAGA 
GAAGACAATC GTGAAAATGG AAAAATTCCA CAAGATGAGC AACTGCATGT CTTACCCCTT 
TATAAAGTAT CCAATGTGGA TGAGTTTGGC AGTTCTGAGA GTCAGGAAGA GAAGAAAAGA 
ACTGGTGCTA TTCAAGTTCT CACATCTTTC CGAAGGCAAG TGCGAATGCT AGCTGAGCCT 
GTAAAAACTT GCCGGCAAAA GAAGTTGGAA AAACAAGAAG CCAAGAAAGC AGCAGCAGAG 
AAGTTAAATG CCGCTTTAGA AAATGGTCAC AGCAAGAACG AAAAGGAGAA GTCCTCACGG 
CATAAAAACG CACATGTAGA AGCTTCTACG CAGGACAAAC AGCAAGCAGA ATTTTCTCGA 
ATGCCAGCCT TGCAACAAAA CACCCTTCTC AATATGAACC ATAGGAACTC TTTAAACCAT 
TATCCATCAT CCGCCCCTAC AAATCCTTAC ACACGAATTC CTAATCTGCC TGACCCATAT 
CAGAATAGTT TTTCTCCCTA CCCTGGATCA AGCCACATGA ATATTTTTCA AACCGCTGCA 
AGCTCATCAG TATCCTGTGC AAATAGCCCT ATGAATTCTT TTCAAGGATC CTTTAATCAA 
GGTAACTCTT GCCCCTCTTA TCCCAGTAAT GGAAATGCCT CCTTGCACAA CTGTCCCCCT 
TATCTTGACT CTTCACAGCA ACAATCCATG GATTATTACA AGTTTAAAAA CCAAGATTCA 
ATGGCTAAAT TAGGTGTGCC CCCAATTCAA TCATTGTACC AGCAGGGGTT TGCTAATAAT 
CAGGCTTATG GTAACAAAAT GTTTAGTTAT GGAAACCAAA GAGTGCAAGC TGATGATTTC 
AGTGGCTGTA ATGCTTATCC AGCACCTCAG GCTGATATAA TGAATCCAGC CACAAGTTCA 
CGGCCTATTA CCAGTAATCA AGACTTCTCT TCTGCGACTA AGAATGGTGA ATATCCTCTT 
CCACACTCCT TCCAGGGTCA TGAAAGCAAC ATGTCATCAA GTATATTGGG AGGCAGAGCT 
GGTTCTCTGC CATTCCCTGG TAAACAGAGT GAAATGCATT CACGCACAGC AAATGGAACA 
TCGGAAATGC TTCCAGGTCT TAACCATGAT AGAACTAATT TTGGAGGCGC AGGCAGAACT 
AATGGTGCTA ATGTTCAGGG AAAGCTACCG TACACCTTAC CTCCTGTACC AGAAGAGCCT 
GCAGAAGTTT GGTCAGACAG TGAACAGAAT TTTCTTGATC CTGATATAGG AGGTGTAGCA 
GTTTGCCCAA CTCATGGGTC AATCTTGATT GAATGTGCAA AGCGTGAGCT CCACGCAACA 
ACACCTTTAA AGAATCCCAA CAGAAATCAC CCTACCAGAA TATCTTTGGT GTTCTACCAA 
CACAAGAGCA TGAACGAGCC AAAGCATGGC CTGGCATTGT GGGAAGCAAA AATGGCAGGC 
AAAGCCAGGG AAAAGGAAGA AGATTGTGAA AGGTATGGTC CAGATTATGT GGCTCCTAAA 
TCTTACAACA GGAAAGCAAA GCGGGAGCCT GTGGAGCCAA TACATGAACC TGCTGAACCA 
ACTTATCTTC GTTTCATCAA GTCACTTACG CAGAGGACGA TGTCAATAAC CACAGACTCT 
CAAGTCACTA CATCTCCATA TGCCTTAACA CGGGTTACAG GTCCTTATAA CAGATATATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951745.1
CDS	574..5856
Translation	MEEDRTNHDEGNRLSPTLLYSSSACQNKNGLLQNGNLPTDKDLLVNGDICLPTKNNYNVAYMKGSQEHGASALIHDNKAYPNHIQNGGIKRTYSEPLFSESQLKKVRTDEINGENKGDQRQNNIQASVSEFCNEDQSVRIFQPVNSPALQVPNEQKKDLNYSNRDIVSCLKDKTVPMCNGATASVSSMENVNNELLEKLPSQYYPEHVSTVVHNNTSLANAINTLNDASNDLSTLATHPSHTSGQMTPQQTSVLPQVSGAIVDGAPNSDHGSEPLVLPGSYSLNRQEQKPLSQETLNYNSHNLVGSMGDACKSMGQMSAQCLDVSGNGSFHIQNSSSNEIASELEAIKKDPAPSLQLHHIMPNKQNESSISQSHDGHPIASQTNESCHSHDPRVSPHPNDQQLQNERTAMFPLTRLPALHSSVNEILAKHKFHNSLEMQNEHLRQIKVMQNCNGTSKDDSFIKSTKEFQSNPFLKSGWLELRPNQRLKEGSSETSCENLLNLLLQSQTNNKKKLRQYPGKQGLANRKCNSQDSEEHLQPNISEQQPNFPKHLSQDSQYQPYSQKTNNGLMWPDLEQASEGTQLGDVQHFLQAQPSEYIEELLKQHFDQSLRNCNQNRDSPLNHFKLEEGAQAANQYQKHHPYPQQFEMEPKQSLSNMNSPFSQIQENNTVNPNHSYPDKQIYLSQKTENSCGFAENKPDPLQYMQYYQANSTEQQEISNYEHDLSARIAKQTHCLPESFSSSPHLHDKMGYYSQFENQVDCIPKHAALRNHLLQRSRSVKADRLVKAEKSSHSNACMHTPMESKTKKNTDKKIKQETRHSSTEAGQQKSIIETMEQQMRHYPGKSSFHRQVSSIKSPKHVKVESSGPVTVLTTHTNFSTLDQQTVPPHEKTPTKRAAGSALHNFIESPSNLLSTPVKNLLDTPIKTQYDFPSCSCVDQIIEKDEGPYYTHLGAGPNVAAIREMMEERFGQKGNAIRIERVVYTGKEGKSAQGCPIAKWVIRRSGTDEKMLCLVRERAGHSCETAVIVILILVWEGISFSLADRLYSELTETLNKYGTLTNRRCARNEERTCACQGLDSETCGASFSFGCSWSMYYNGCKFARSKVPRKFKLLGDDPKSEEKLEANLQHLSTLMAPIYKKLAPDAYHNQIEHEHRAPDCRLGLKEGRPFSGVTACLDFCAHSHRDLHNMQNGSTLVCTLTREDNRENGKIPQDEQLHVLPLYKVSNVDEFGSSESQEEKKRTGAIQVLTSFRRQVRMLAEPVKTCRQKKLEKQEAKKAAAEKLNAALENGHSKNEKEKSSRHKNAHVEASTQDKQQAEFSRMPALQQNTLLNMNHRNSLNHYPSSAPTNPYTRIPNLPDPYQNSFSPYPGSSHMNIFQTAASSSVSCANSPMNSFQGSFNQGNSCPSYPSNGNASLHNCPPYLDSSQQQSMDYYKFKNQDSMAKLGVPPIQSLYQQGFANNQAYGNKMFSYGNQRVQADDFSGCNAYPAPQADIMNPATSSRPITSNQDFSSATKNGEYPLPHSFQGHESNMSSSILGGRAGSLPFPGKQSEMHSRTANGTSEMLPGLNHDRTNFGGAGRTNGANVQGKLPYTLPPVPEEPAEVWSDSEQNFLDPDIGGVAVCPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAGKAREKEEDCERYGPDYVAPKSYNRKAKREPVEPIHEPAEPTYLRFIKSLTQRTMSITTDSQVTTSPYALTRVTGPYNRYI

Target ORF information:

RefSeq Version	XM_018096256.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tet methylcytosine dioxygenase 2 (tet2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096256.1

ORF Insert Sequence:

ATGGAAGAGG ACAGAACCAA CCATGACGAG GGCAATAGAT TGAGTCCAAC CCTATTATAT 
TCTTCGTCAG CTTGCCAAAA CAAGAACGGT CTATTACAAA ATGGGAACCT GCCAACAGAC 
AAAGACCTTC TAGTTAATGG GGACATATGT TTACCAACCA AAAACAACTA TAATGTTGCT 
TACATGAAGG GAAGTCAAGA ACATGGTGCT TCTGCTCTCA TCCATGACAA CAAAGCTTAT 
CCCAACCACA TACAAAATGG TGGAATAAAG CGCACATATA GTGAACCGTT GTTTTCTGAA 
TCCCAGTTGA AAAAGGTCAG AACAGATGAG ATTAATGGAG AAAATAAGGG TGACCAAAGG 
CAAAATAATA TTCAAGCAAG TGTCTCTGAG TTCTGCAATG AAGATCAGTC AGTAAGAATT 
TTCCAACCAG TTAATTCACC AGCTCTTCAG GTTCCAAATG AGCAGAAGAA AGACCTTAAT 
TACAGCAACA GAGACATTGT CTCATGTCTT AAAGACAAGA CAGTGCCAAT GTGTAATGGT 
GCTACTGCTT CTGTATCTTC CATGGAAAAC GTGAATAATG AGCTCCTGGA AAAACTGCCT 
TCTCAATATT ATCCTGAACA TGTTTCTACT GTGGTGCATA ATAACACATC TCTTGCAAAT 
GCCATTAATA CGCTAAATGA TGCTAGCAAT GATTTGTCTA CTCTGGCAAC TCATCCGTCA 
CATACCTCAG GGCAGATGAC TCCTCAACAA ACTTCGGTGC TGCCTCAAGT GTCAGGTGCA 
ATTGTTGATG GGGCCCCCAA TAGTGACCAT GGTAGTGAGC CACTTGTATT ACCAGGAAGC 
TATTCACTTA ATAGACAAGA ACAGAAGCCA CTTTCACAGG AAACTCTTAA TTATAATTCC 
CATAATTTAG TTGGAAGCAT GGGTGATGCA TGCAAATCCA TGGGCCAGAT GTCAGCACAA 
TGTCTAGATG TTTCTGGTAA TGGTTCATTT CATATCCAAA ATAGCTCATC AAATGAGATA 
GCTTCTGAAT TAGAGGCCAT CAAGAAAGAT CCAGCACCAT CACTGCAATT ACATCATATT 
ATGCCCAACA AGCAAAATGA GTCCAGTATA AGTCAATCAC ATGATGGGCA TCCAATTGCA 
TCACAAACTA ATGAATCTTG CCATTCACAT GATCCAAGAG TATCTCCACA TCCTAATGAT 
CAACAGTTAC AAAATGAAAG AACGGCCATG TTTCCTCTCA CCAGATTACC TGCACTACAC 
AGTTCGGTTA ATGAAATTCT AGCAAAACAC AAGTTTCACA ACAGTTTAGA GATGCAAAAT 
GAGCATCTCA GGCAAATCAA AGTCATGCAG AATTGCAATG GGACAAGTAA AGATGATTCT 
TTCATTAAGT CTACAAAAGA ATTTCAATCC AATCCTTTTT TAAAATCAGG CTGGTTAGAA 
CTTCGCCCTA ATCAGCGCCT AAAAGAAGGA TCTTCTGAAA CTTCTTGTGA AAATTTACTG 
AACTTACTTC TCCAGTCCCA GACTAATAAC AAGAAAAAGC TAAGACAATA CCCTGGAAAA 
CAAGGTTTAG CAAATAGAAA GTGTAACAGT CAAGATAGTG AAGAACATCT GCAGCCAAAT 
ATTTCTGAGC AGCAGCCAAA TTTCCCAAAG CACTTATCAC AAGATTCTCA ATACCAGCCA 
TACAGTCAAA AAACTAATAA TGGTCTGATG TGGCCTGATC TTGAACAAGC TTCTGAGGGC 
ACTCAGTTAG GTGATGTCCA ACATTTTTTG CAAGCACAGC CAAGTGAATA TATTGAAGAG 
CTGTTAAAAC AACATTTTGA TCAGTCACTC CGTAACTGTA ACCAAAATAG GGACAGTCCT 
CTTAACCACT TTAAACTTGA AGAAGGTGCT CAGGCAGCTA ATCAGTATCA AAAACACCAT 
CCATATCCTC AGCAATTTGA GATGGAACCT AAGCAAAGTT TAAGCAACAT GAATTCCCCC 
TTCAGTCAAA TTCAAGAAAA TAATACAGTT AACCCTAACC ATTCCTATCC TGACAAACAA 
ATATATCTGT CACAAAAAAC AGAAAATAGC TGTGGTTTTG CAGAGAACAA ACCTGACCCT 
TTGCAATACA TGCAGTATTA TCAAGCAAAT AGTACTGAGC AACAAGAAAT AAGCAATTAT 
GAGCACGATC TTTCGGCCCG AATAGCAAAG CAGACACATT GCCTGCCAGA GAGCTTTAGT 
TCATCCCCAC ATTTGCATGA TAAAATGGGA TATTATTCTC AATTTGAAAA CCAGGTTGAC 
TGTATTCCAA AGCATGCTGC TCTTAGGAAT CACTTGCTGC AACGAAGTAG ATCAGTTAAA 
GCTGATAGAT TGGTTAAAGC TGAGAAGAGT TCCCACTCTA ATGCCTGCAT GCATACACCT 
ATGGAAAGCA AAACTAAAAA AAACACAGAT AAAAAAATCA AACAAGAAAC TCGCCATTCA 
AGCACAGAGG CTGGACAACA AAAGAGTATT ATTGAAACAA TGGAACAGCA GATGAGACAT 
TATCCTGGTA AATCATCGTT TCATCGTCAA GTATCAAGTA TCAAATCTCC CAAGCATGTG 
AAGGTGGAGT CTTCAGGGCC TGTCACAGTC CTAACTACAC ACACTAATTT TTCTACACTA 
GACCAACAAA CTGTTCCTCC GCATGAGAAA ACACCAACCA AAAGAGCAGC AGGTTCTGCT 
CTACATAACT TTATAGAATC ACCCTCAAAT CTACTCAGTA CACCAGTAAA AAATCTTTTG 
GACACACCTA TAAAAACGCA GTATGATTTT CCATCATGCA GTTGTGTAGA TCAAATAATT 
GAAAAAGATG AAGGACCTTA TTATACACAT CTTGGTGCTG GACCCAATGT GGCAGCTATC 
AGAGAAATGA TGGAAGAAAG GTTTGGCCAA AAGGGGAATG CCATCAGAAT TGAGAGGGTT 
GTTTATACAG GAAAAGAAGG CAAAAGTGCT CAGGGATGTC CTATTGCTAA ATGGGTGATT 
CGGCGTAGTG GGACAGATGA GAAAATGCTG TGTTTGGTGA GGGAACGTGC TGGTCACTCC 
TGCGAAACAG CAGTGATTGT CATTCTGATC TTGGTTTGGG AAGGCATTTC ATTCTCTTTG 
GCAGACAGGC TTTACTCAGA GCTCACAGAA ACGCTAAATA AGTATGGAAC CTTAACAAAC 
CGACGCTGTG CACGTAACGA AGAACGTACC TGTGCATGCC AGGGTCTGGA CTCTGAAACC 
TGCGGTGCCT CCTTTTCCTT TGGGTGCTCA TGGAGTATGT ATTATAACGG GTGTAAATTC 
GCTAGAAGTA AAGTACCAAG AAAATTCAAA CTCCTTGGTG ATGATCCAAA ATCGGAAGAA 
AAGTTGGAAG CTAATTTACA GCACTTATCA ACACTGATGG CTCCAATTTA CAAGAAGCTT 
GCCCCTGATG CCTATCATAA CCAGATTGAG CATGAGCACA GAGCTCCAGA TTGCCGTCTT 
GGTTTGAAGG AAGGTCGACC ATTTTCTGGG GTTACTGCTT GCTTGGATTT TTGCGCTCAT 
TCCCACCGGG ACTTGCACAA CATGCAAAAT GGCAGCACAC TGGTTTGCAC TTTAACCAGA 
GAAGACAATC GTGAAAATGG AAAAATTCCA CAAGATGAGC AACTGCATGT CTTACCCCTT 
TATAAAGTAT CCAATGTGGA TGAGTTTGGC AGTTCTGAGA GTCAGGAAGA GAAGAAAAGA 
ACTGGTGCTA TTCAAGTTCT CACATCTTTC CGAAGGCAAG TGCGAATGCT AGCTGAGCCT 
GTAAAAACTT GCCGGCAAAA GAAGTTGGAA AAACAAGAAG CCAAGAAAGC AGCAGCAGAG 
AAGTTAAATG CCGCTTTAGA AAATGGTCAC AGCAAGAACG AAAAGGAGAA GTCCTCACGG 
CATAAAAACG CACATGTAGA AGCTTCTACG CAGGACAAAC AGCAAGCAGA ATTTTCTCGA 
ATGCCAGCCT TGCAACAAAA CACCCTTCTC AATATGAACC ATAGGAACTC TTTAAACCAT 
TATCCATCAT CCGCCCCTAC AAATCCTTAC ACACGAATTC CTAATCTGCC TGACCCATAT 
CAGAATAGTT TTTCTCCCTA CCCTGGATCA AGCCACATGA ATATTTTTCA AACCGCTGCA 
AGCTCATCAG TATCCTGTGC AAATAGCCCT ATGAATTCTT TTCAAGGATC CTTTAATCAA 
GGTAACTCTT GCCCCTCTTA TCCCAGTAAT GGAAATGCCT CCTTGCACAA CTGTCCCCCT 
TATCTTGACT CTTCACAGCA ACAATCCATG GATTATTACA AGTTTAAAAA CCAAGATTCA 
ATGGCTAAAT TAGGTGTGCC CCCAATTCAA TCATTGTACC AGCAGGGGTT TGCTAATAAT 
CAGGCTTATG GTAACAAAAT GTTTAGTTAT GGAAACCAAA GAGTGCAAGC TGATGATTTC 
AGTGGCTGTA ATGCTTATCC AGCACCTCAG GCTGATATAA TGAATCCAGC CACAAGTTCA 
CGGCCTATTA CCAGTAATCA AGACTTCTCT TCTGCGACTA AGAATGGTGA ATATCCTCTT 
CCACACTCCT TCCAGGGTCA TGAAAGCAAC ATGTCATCAA GTATATTGGG AGGCAGAGCT 
GGTTCTCTGC CATTCCCTGG TAAACAGAGT GAAATGCATT CACGCACAGC AAATGGAACA 
TCGGAAATGC TTCCAGGTCT TAACCATGAT AGAACTAATT TTGGAGGCGC AGGCAGAACT 
AATGGTGCTA ATGTTCAGGG AAAGCTACCG TACACCTTAC CTCCTGTACC AGAAGAGCCT 
GCAGAAGTTT GGTCAGACAG TGAACAGAAT TTTCTTGATC CTGATATAGG AGGTGTAGCA 
GTTTGCCCAA CTCATGGGTC AATCTTGATT GAATGTGCAA AGCGTGAGCT CCACGCAACA 
ACACCTTTAA AGAATCCCAA CAGAAATCAC CCTACCAGAA TATCTTTGGT GTTCTACCAA 
CACAAGAGCA TGAACGAGCC AAAGCATGGC CTGGCATTGT GGGAAGCAAA AATGGCAGGC 
AAAGCCAGGG AAAAGGAAGA AGATTGTGAA AGGTATGGTC CAGATTATGT GGCTCCTAAA 
TCTTACAACA GGAAAGCAAA GCGGGAGCCT GTGGAGCCAA TACATGAACC TGCTGAACCA 
ACTTATCTTC GTTTCATCAA GTCACTTACG CAGAGGACGA TGTCAATAAC CACAGACTCT 
CAAGTCACTA CATCTCCATA TGCCTTAACA CGGGTTACAG GTCCTTATAA CAGATATATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

tet2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム