pus1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pus1
Entrez Gene ID	100380023
Full Name	pseudouridylate synthase 1
General protein information	Preferred Names pseudouridylate synthase 1 Names tRNA pseudouridine synthase A, mitochondrial
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002931876.5	XP_002931922.1	tRNA pseudouridine synthase A
XM_002931876.4	XP_002931922.1	tRNA pseudouridine synthase A, mitochondrial

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pus1	NP_001020732.1
Neurospora crassa	NCU07528	XP_959041.1
Xenopus tropicalis (tropical clawed frog)	pus1	XP_002931922.1
Homo sapiens (human)	PUS1	NP_079491.2
Caenorhabditis elegans (roundworm)	pus-1	NP_507242.1
Schizosaccharomyces pombe (fission yeast)	pus1	NP_588446.1
Pan troglodytes (chimpanzee)	PUS1	XP_001139560.1
Bos taurus (cattle)	PUS1	XP_002694500.1
Rattus norvegicus (Norway rat)	Pus1	NP_001020734.1
Canis lupus familiaris (dog)	PUS1	XP_005636135.1
Gallus gallus (chicken)	PUS1	XP_415090.1
Saccharomyces cerevisiae (baker's yeast)	PUS1	NP_015112.1
Kluyveromyces lactis	KLLA0B02244g	XP_451631.1
Eremothecium gossypii	AGOS_AFL105C	NP_985445.2
Oryza sativa (rice)	Os02g0668400	NP_001047686.1
	LOC719360	XP_001113470.1
Danio rerio (zebrafish)	pus1	NP_001008603.1
Drosophila melanogaster (fruit fly)	CG4159	NP_001163664.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002409	XP_312543.3
Magnaporthe oryzae (rice blast fungus)	MGG_07663	XP_003711599.1
Arabidopsis thaliana (thale cress)	AT1G20370	NP_564112.1
Arabidopsis thaliana (thale cress)	AT1G76120	NP_565126.1

Gene Ontology

The following pus1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pus1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa03585	XM_002931876.4 Latest version!	Xenopus tropicalis pseudouridylate synthase 1 (pus1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OXa48954	XM_002931876.5 Latest version!	Xenopus tropicalis pseudouridylate synthase 1 (pus1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pus1 ( XM_002931876.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931922.1 Xenopus tropicalis pseudouridylate synthase 1 (pus1), mRNA.

Price & Availability↑

CloneID	OXa03585
Clone ID Related Accession (Same CDS sequence)	XM_002931876.4
Accession Version	XM_002931876.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA pseudouridine synthase A, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931876.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATC CCGAGAAAGA GTCTTTGTTA AAAAACGTTA CCACAGGGGA CATCAGGAAG 
GCATGTGTTC TCCAGGAGGA GTCGGAAGGA CAGGAAAAAA ATGGACACCA AGCTAAGAAA 
TTTAAGGCTG ATCAAGAGGA GCTGAGTAAG AAGCATCCTA AACGGAAAGT AGTTCTCTTA 
ATGGCCTACT CTGGGAAAGG CTACCATGGC ATGCAGAGAA ATGTGGGTTC CTCCCAGTTT 
AAGACCATAG AGGATGAACT TGTTCAAGCA TTGGTTTTGT CTGGATGTAT CCCTGACAAT 
CATGCAGAAG ACATGAAAAA GATGTCATTT CAACGTTGTG CTCGCACAGA TAAAGGGGTT 
TCCGCTGCTG GACAAGTTGT ATCCCTAAAG ATTTGGTTGA TAGATAATGT AGTTGAAAAG 
ATTAATGAAA ATCTGCCCTC TCATATTAGG ATACTTGGAT TGAAGCGAGT AACAGGCAGG 
TTTAACTCAA AGAACAGCTG TGACGCTCGC ACCTACTCTT ACACATTGCC AACCTTCGCT 
TTCTCACATA AAGACAAAGA GGCACAAGAT GAAAACTTTC GACTGAGTCA GGAAACGCTA 
AACAGAGTAA ATGAACTTCT TGCACTTTAC AAAGGCACTC GCAACTTTCA CAACTTTACC 
TCTCAGAAAG GACCAAGAGA TCCTAGTGCA AAGCGCTATA TCATGGAAAT GCATTGTGAG 
CCTCCTTTTC AGCAAGGGGG GCTGGAGTTA GCGGTGATCA AGGTAAAAGG CCAAAGCTTT 
ATGATGCACC AGATCCGCAA AATGATTGGA TTAGTCATTG CTGTGGTGAA GGGCTTTGCT 
CCCACATCCA TCATAGAACG TAGCTGGGGA GAAGAAAAAG TGGACATTCC TAAAGCTCCT 
GGTTTAGGTC TGGTACTTGA GAGGGTTCAC TTTGAAAAGT ACAACAGACG CTTTGGAAAT 
GATGGACTTC ATGAATCTCT GAATTGGACA GAAGAAGAAG AAAAAATTGA GTCTTTTAAG 
AAGGAGCATA TTTACCCCAC TATTATTCAG ACGGAAATTG ACGAGAAGTC TATGCTTGCT 
TGGCTCGCCA CTCTGCCCAT CCATGACTAT GATGCCACAT CTGTACAACA AAACCAAGAC 
AACCATAAGG CAGAAGGAGC AGTGGCTGGA ATCAGTGATG AAGAGATAGA ATCGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931922.1
CDS	486..1685
Translation	MADPEKESLLKNVTTGDIRKACVLQEESEGQEKNGHQAKKFKADQEELSKKHPKRKVVLLMAYSGKGYHGMQRNVGSSQFKTIEDELVQALVLSGCIPDNHAEDMKKMSFQRCARTDKGVSAAGQVVSLKIWLIDNVVEKINENLPSHIRILGLKRVTGRFNSKNSCDARTYSYTLPTFAFSHKDKEAQDENFRLSQETLNRVNELLALYKGTRNFHNFTSQKGPRDPSAKRYIMEMHCEPPFQQGGLELAVIKVKGQSFMMHQIRKMIGLVIAVVKGFAPTSIIERSWGEEKVDIPKAPGLGLVLERVHFEKYNRRFGNDGLHESLNWTEEEEKIESFKKEHIYPTIIQTEIDEKSMLAWLATLPIHDYDATSVQQNQDNHKAEGAVAGISDEEIESD

Target ORF information:

RefSeq Version	XM_002931876.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pseudouridylate synthase 1 (pus1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931876.4

ORF Insert Sequence:

ATGGCTGATC CCGAGAAAGA GTCTTTGTTA AAAAACGTTA CCACAGGGGA CATCAGGAAG 
GCATGTGTTC TCCAGGAGGA GTCGGAAGGA CAGGAAAAAA ATGGACACCA AGCTAAGAAA 
TTTAAGGCTG ATCAAGAGGA GCTGAGTAAG AAGCATCCTA AACGGAAAGT AGTTCTCTTA 
ATGGCCTACT CTGGGAAAGG CTACCATGGC ATGCAGAGAA ATGTGGGTTC CTCCCAGTTT 
AAGACCATAG AGGATGAACT TGTTCAAGCA TTGGTTTTGT CTGGATGTAT CCCTGACAAT 
CATGCAGAAG ACATGAAAAA GATGTCATTT CAACGTTGTG CTCGCACAGA TAAAGGGGTT 
TCCGCTGCTG GACAAGTTGT ATCCCTAAAG ATTTGGTTGA TAGATAATGT AGTTGAAAAG 
ATTAATGAAA ATCTGCCCTC TCATATTAGG ATACTTGGAT TGAAGCGAGT AACAGGCAGG 
TTTAACTCAA AGAACAGCTG TGACGCTCGC ACCTACTCTT ACACATTGCC AACCTTCGCT 
TTCTCACATA AAGACAAAGA GGCACAAGAT GAAAACTTTC GACTGAGTCA GGAAACGCTA 
AACAGAGTAA ATGAACTTCT TGCACTTTAC AAAGGCACTC GCAACTTTCA CAACTTTACC 
TCTCAGAAAG GACCAAGAGA TCCTAGTGCA AAGCGCTATA TCATGGAAAT GCATTGTGAG 
CCTCCTTTTC AGCAAGGGGG GCTGGAGTTA GCGGTGATCA AGGTAAAAGG CCAAAGCTTT 
ATGATGCACC AGATCCGCAA AATGATTGGA TTAGTCATTG CTGTGGTGAA GGGCTTTGCT 
CCCACATCCA TCATAGAACG TAGCTGGGGA GAAGAAAAAG TGGACATTCC TAAAGCTCCT 
GGTTTAGGTC TGGTACTTGA GAGGGTTCAC TTTGAAAAGT ACAACAGACG CTTTGGAAAT 
GATGGACTTC ATGAATCTCT GAATTGGACA GAAGAAGAAG AAAAAATTGA GTCTTTTAAG 
AAGGAGCATA TTTACCCCAC TATTATTCAG ACGGAAATTG ACGAGAAGTC TATGCTTGCT 
TGGCTCGCCA CTCTGCCCAT CCATGACTAT GATGCCACAT CTGTACAACA AAACCAAGAC 
AACCATAAGG CAGAAGGAGC AGTGGCTGGA ATCAGTGATG AAGAGATAGA ATCGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pus1 ( XM_002931876.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931922.1 Xenopus tropicalis pseudouridylate synthase 1 (pus1), mRNA.

Price & Availability↑

CloneID	OXa48954
Clone ID Related Accession (Same CDS sequence)	XM_002931876.5
Accession Version	XM_002931876.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tRNA pseudouridine synthase A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931876.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATC CCGAGAAAGA GTCTTTGTTA AAAAACGTTA CCACAGGGGA CATCAGGAAG 
GCATGTGTTC TCCAGGAGGA GTCGGAAGGA CAGGAAAAAA ATGGACACCA AGCTAAGAAA 
TTTAAGGCTG ATCAAGAGGA GCTGAGTAAG AAGCATCCTA AACGGAAAGT AGTTCTCTTA 
ATGGCCTACT CTGGGAAAGG CTACCATGGC ATGCAGAGAA ATGTGGGTTC CTCCCAGTTT 
AAGACCATAG AGGATGAACT TGTTCAAGCA TTGGTTTTGT CTGGATGTAT CCCTGACAAT 
CATGCAGAAG ACATGAAAAA GATGTCATTT CAACGTTGTG CTCGCACAGA TAAAGGGGTT 
TCCGCTGCTG GACAAGTTGT ATCCCTAAAG ATTTGGTTGA TAGATAATGT AGTTGAAAAG 
ATTAATGAAA ATCTGCCCTC TCATATTAGG ATACTTGGAT TGAAGCGAGT AACAGGCAGG 
TTTAACTCAA AGAACAGCTG TGACGCTCGC ACCTACTCTT ACACATTGCC AACCTTCGCT 
TTCTCACATA AAGACAAAGA GGCACAAGAT GAAAACTTTC GACTGAGTCA GGAAACGCTA 
AACAGAGTAA ATGAACTTCT TGCACTTTAC AAAGGCACTC GCAACTTTCA CAACTTTACC 
TCTCAGAAAG GACCAAGAGA TCCTAGTGCA AAGCGCTATA TCATGGAAAT GCATTGTGAG 
CCTCCTTTTC AGCAAGGGGG GCTGGAGTTA GCAGTGATCA AGGTAAAAGG CCAAAGCTTT 
ATGATGCACC AGATCCGCAA AATGATTGGA TTAGTCATTG CTGTGGTGAA GGGCTTTGCT 
CCCACATCCA TCATAGAACG TAGCTGGGGA GAAGAAAAAG TGGACATTCC TAAAGCTCCT 
GGTTTAGGTC TGGTACTTGA GAGGGTTCAC TTTGAAAAGT ACAACAGACG CTTTGGAAAT 
GATGGACTTC ATGAATCTCT GAATTGGACA GAAGAAGAAG AAAAAATTGA GTCTTTTAAG 
AAGGAGCATA TTTACCCCAC TATTATTCAG ACGGAAATTG ACGAGAAGTC TATGCTTGCT 
TGGCTCGCCA CTCTGCCCAT CCATGACTAT GATGCCACAT CTGTACAACA AAACCAAGAC 
AACCATAAGG CAGAAGGAGC AGTGGCTGGA ATCAGTGATG AAGAGATAGA ATCGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931922.1
CDS	506..1705
Translation	MADPEKESLLKNVTTGDIRKACVLQEESEGQEKNGHQAKKFKADQEELSKKHPKRKVVLLMAYSGKGYHGMQRNVGSSQFKTIEDELVQALVLSGCIPDNHAEDMKKMSFQRCARTDKGVSAAGQVVSLKIWLIDNVVEKINENLPSHIRILGLKRVTGRFNSKNSCDARTYSYTLPTFAFSHKDKEAQDENFRLSQETLNRVNELLALYKGTRNFHNFTSQKGPRDPSAKRYIMEMHCEPPFQQGGLELAVIKVKGQSFMMHQIRKMIGLVIAVVKGFAPTSIIERSWGEEKVDIPKAPGLGLVLERVHFEKYNRRFGNDGLHESLNWTEEEEKIESFKKEHIYPTIIQTEIDEKSMLAWLATLPIHDYDATSVQQNQDNHKAEGAVAGISDEEIESD

Target ORF information:

RefSeq Version	XM_002931876.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pseudouridylate synthase 1 (pus1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931876.5

ORF Insert Sequence:

ATGGCTGATC CCGAGAAAGA GTCTTTGTTA AAAAACGTTA CCACAGGGGA CATCAGGAAG 
GCATGTGTTC TCCAGGAGGA GTCGGAAGGA CAGGAAAAAA ATGGACACCA AGCTAAGAAA 
TTTAAGGCTG ATCAAGAGGA GCTGAGTAAG AAGCATCCTA AACGGAAAGT AGTTCTCTTA 
ATGGCCTACT CTGGGAAAGG CTACCATGGC ATGCAGAGAA ATGTGGGTTC CTCCCAGTTT 
AAGACCATAG AGGATGAACT TGTTCAAGCA TTGGTTTTGT CTGGATGTAT CCCTGACAAT 
CATGCAGAAG ACATGAAAAA GATGTCATTT CAACGTTGTG CTCGCACAGA TAAAGGGGTT 
TCCGCTGCTG GACAAGTTGT ATCCCTAAAG ATTTGGTTGA TAGATAATGT AGTTGAAAAG 
ATTAATGAAA ATCTGCCCTC TCATATTAGG ATACTTGGAT TGAAGCGAGT AACAGGCAGG 
TTTAACTCAA AGAACAGCTG TGACGCTCGC ACCTACTCTT ACACATTGCC AACCTTCGCT 
TTCTCACATA AAGACAAAGA GGCACAAGAT GAAAACTTTC GACTGAGTCA GGAAACGCTA 
AACAGAGTAA ATGAACTTCT TGCACTTTAC AAAGGCACTC GCAACTTTCA CAACTTTACC 
TCTCAGAAAG GACCAAGAGA TCCTAGTGCA AAGCGCTATA TCATGGAAAT GCATTGTGAG 
CCTCCTTTTC AGCAAGGGGG GCTGGAGTTA GCAGTGATCA AGGTAAAAGG CCAAAGCTTT 
ATGATGCACC AGATCCGCAA AATGATTGGA TTAGTCATTG CTGTGGTGAA GGGCTTTGCT 
CCCACATCCA TCATAGAACG TAGCTGGGGA GAAGAAAAAG TGGACATTCC TAAAGCTCCT 
GGTTTAGGTC TGGTACTTGA GAGGGTTCAC TTTGAAAAGT ACAACAGACG CTTTGGAAAT 
GATGGACTTC ATGAATCTCT GAATTGGACA GAAGAAGAAG AAAAAATTGA GTCTTTTAAG 
AAGGAGCATA TTTACCCCAC TATTATTCAG ACGGAAATTG ACGAGAAGTC TATGCTTGCT 
TGGCTCGCCA CTCTGCCCAT CCATGACTAT GATGCCACAT CTGTACAACA AAACCAAGAC 
AACCATAAGG CAGAAGGAGC AGTGGCTGGA ATCAGTGATG AAGAGATAGA ATCGGATTGA