gcfc2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gcfc2
Entrez Gene ID	100216244
Full Name	GC-rich sequence DNA-binding factor 2
Synonyms	c2orf3
General protein information	Preferred Names GC-rich sequence DNA-binding factor 2 Names GC-rich sequence DNA-binding factor 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935987.5	XP_002936033.2	GC-rich sequence DNA-binding factor 2
XM_002935987.4	XP_002936033.2	GC-rich sequence DNA-binding factor 2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC101734739	XP_004918235.1
	C2orf3	XP_001106597.2
Mus musculus (house mouse)	Gcfc2	NP_808552.2
Danio rerio (zebrafish)	gcfc2	NP_001103577.2
Xenopus tropicalis (tropical clawed frog)	gcfc2	XP_002936033.2
Homo sapiens (human)	GCFC2	NP_003194.3
Pan troglodytes (chimpanzee)	GCFC2	XP_001145277.2
Bos taurus (cattle)	GCFC2	XP_003586682.1
Canis lupus familiaris (dog)	GCFC2	XP_540209.3
Rattus norvegicus (Norway rat)	Gcfc2	NP_001128026.1

Gene Ontology

The following gcfc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gcfc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa16654	XM_002935987.5 Latest version!	Xenopus tropicalis GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa16654	XM_002935987.4	Xenopus tropicalis GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gcfc2 ( XM_002935987.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936033.2 Xenopus tropicalis GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.

Price & Availability↑

CloneID	OXa16654
Clone ID Related Accession (Same CDS sequence)	XM_002935987.5 , XM_002935987.4
Accession Version	XM_002935987.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	GC-rich sequence DNA-binding factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935987.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGAA AACCGAAGCG AGCGATTCGT GTGAGGAAAG CGGAGTCCAG CGAGGAAGAG 
GAGCGGGAAG AAGAGAGTGA GCAACCCCGG GCCAGGGAGC AGAGGAGGCC AGCCGGCCGC 
GGCTTATTTT GTGGGACTGA GCGAGGGAAG AGGGCCGAAG CGGCGCAGGG AGACGGGGCT 
GGGAGTGATG CGGCGCGGAG AGACGGGGCT GGGAGTGATG CGGCGCGGAG AGACGGGGCT 
GGGAGTGATG CGGCGCGGAG AGACGGGGCT GGGAGTGATG CGGAAGGGGA ACCGGATCCC 
AGTGAAACAG TCGGGACTGC TCCTGTGGAA CCCGAATTCT CTATTCGCTT TACAGCCCCG 
CCCACCCTGC TGAGCTTCGC TGAGGAGAGA GAAGATGCAG ACTTCAAGAT CAAGAAGCCC 
AGTGTCAATG CAGTGGTATT TCAAGTGCAG AAAAAGACAG AAAAGAACTC CATAATTAAG 
TCCTATAAAA AAGAAAGAGC AGCCAAGACA GAAAGTGACA GTGACTCTGA GGTGGAGGGT 
CTGGAAGAAG ATGGAGCAAA GTCCAAGAGT GACAGCAGCT CTGAGCTGGA AGATCAGAGA 
GAAGATGGAG CAAAATCAGA GAGTAACAGT AGCTCAGAGG TGGAGAATAA AGACCCTCAG 
CCACAAGAAA GGTCAGACTC TCCAAACTCT GGAATGGGCA GCTCACCTAC ATCTCCTGAC 
TCTGGCATCA CAGGTGCTAT TCCAGGCGCT AAGGTCATTC GAGCAGCTAG AAGGCGGCGC 
AGGTTGGCCC GAGCTCAAGG TGATTATATA CCCATTGGCA CAAAGCAGGA AATGTCCAGT 
TCCAGCCAGT CAGAAAGTGA CAGTGACATT GATGATCACG AGAGAAGAAT CAAGTTTGCC 
CCGGGAACAA AGACTTTGCG AGAGCAGATG GAAGAGGAAA TGTCATCAAA CAGTGACTCT 
GAGATGCATA AAAGTGAAGA TGAAGAGGAT TTGCAAGACA AATGGGAGGA ACAGCAAATA 
CGGAAAGCTG TCAAATATCA AAAGGGAATG GATGAGGATT TGCCACAAGT GAGAATTCCA 
CCCAAGAGTA AAAAGAGTGT TGAACCAAGG ATTTCTCTGC CACCAGTAAC TGCTGAAGAC 
ATAAAAAAGA AACTGGCTTC ACGGTTGAAT TCCTTTCATG AAGTCCACCG GGCACATGTT 
GCTGAGCGAG AAAAGTATGT TAGTGATCTG GACAGTGCAA AAACCACACT AGAAAAGCTT 
GAAATGTCTT CCAGTGAGCA AACCTATAAG TTTTTCAAGG AGATGAAAAC CTATGTAGAA 
AACTTTGTTG ACTGTGTGAA TGAAAAGATA GCCCAGATCA ATAGGCTTGA ATTAGAGATG 
ATTGAGATTT TTCAGAAACG AGCTGAATCC TTGAATAAGC GCAGGCAAGA TGACCTTCGG 
AATGAGTCTG TAGCTGTTCA AAATATATCT GGAACTAAAG ATGCCAATGG CCAGACTACA 
GACCTGATGA CTGATTGTGA GTTACGAAGA AAGAAAAGAA GGCAAAAACG GAAGGAGTGT 
GGGGACTCAG ATCACTATGA AGGGATGTCT AGTGATGATG AACTGTCCAC TGATGATGAG 
AGGAGTTTCC AGAAAAACAG AGAGAGCATC AGGGCACAGA GTAAAACCAT ATTTGAGGAT 
GTGCATGAAG ATTTCCACCA GATAAAGAAT ATCCTGTCCA GATTTACAGA GTGGAGAGGG 
AGATTTCCTG AGTCCTATTA CGATGCCTAC ATTAGCCTGT GCCTCCACAA ACTACTGAAT 
CCAATAATCC GAGTGCATCT GTTAGACTGG AATCCCTTGG AGGATAAAAA AGATCTGGAA 
GAGATGACAT GGTACCAAGA CTTAGAAGAG TTTTGCTATA GGGAGAATGA GCTGGAGATG 
AATGACGAGA ATTCGGACCA TAAAGTCCTG TCCGCTGTGA TTGAGAAAAC GGTTATTCCA 
AAAGTTTCAG GATTTGTTGA ACTCTTGTGG GATCCACTAT CCGCTGTACA GACTGACAAC 
CTAGCGCATT TCTGCAAAAC AAATGTCAAG CATAATGAGT CCAGTAAAGC AGTGCAGGGC 
CTGATAAATT GTCTACTTTC AACAATGAAG AAAGCCATTG AAGATGATGT GTTTATTCCT 
CTCTTTCCAA AAAGACTTTT GGAAGACAGA TTCTCTCCGC ATTCAAGATT CCAAGAAAGG 
CGTTTCTGGT CTGCTGTGAA AATGTTCCAG AACGTTCTTT GCTGGGATGG ATTCCTCCAA 
GAAGAGACCC TGCAAGAGCT CAGCCTTGAT AAGCTGCTTA ATCGATACCT ACTGCTAGTA 
ATTCTGAATG CAGAGCCAGG ACCAGACAGT GTGAAGAAGT GCAAGAGAGT GGTTGAATGT 
CTCCCTCAGA GCTGGTTTAG GAACCTTGAG AGTGGATCAT CTCTTCATCG CTTACTGAAT 
TTCAGCAAAC ACCTACTGCA GTCTATTCAT ACTCTGCACA AGCTAAATGA CAGAGAAAAC 
ATGAAGATTT TAGTGTCCCT GCTCTTGAAG ATCAAAGCCG TGGACTACGC AGAGGAAGCC 
ATCAGCCAGT ATAACCTGGA AGAACTGAAA GTGGTCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936033.2
CDS	60..2681
Translation	MFRKPKRAIRVRKAESSEEEEREEESEQPRAREQRRPAGRGLFCGTERGKRAEAAQGDGAGSDAARRDGAGSDAARRDGAGSDAARRDGAGSDAEGEPDPSETVGTAPVEPEFSIRFTAPPTLLSFAEEREDADFKIKKPSVNAVVFQVQKKTEKNSIIKSYKKERAAKTESDSDSEVEGLEEDGAKSKSDSSSELEDQREDGAKSESNSSSEVENKDPQPQERSDSPNSGMGSSPTSPDSGITGAIPGAKVIRAARRRRRLARAQGDYIPIGTKQEMSSSSQSESDSDIDDHERRIKFAPGTKTLREQMEEEMSSNSDSEMHKSEDEEDLQDKWEEQQIRKAVKYQKGMDEDLPQVRIPPKSKKSVEPRISLPPVTAEDIKKKLASRLNSFHEVHRAHVAEREKYVSDLDSAKTTLEKLEMSSSEQTYKFFKEMKTYVENFVDCVNEKIAQINRLELEMIEIFQKRAESLNKRRQDDLRNESVAVQNISGTKDANGQTTDLMTDCELRRKKRRQKRKECGDSDHYEGMSSDDELSTDDERSFQKNRESIRAQSKTIFEDVHEDFHQIKNILSRFTEWRGRFPESYYDAYISLCLHKLLNPIIRVHLLDWNPLEDKKDLEEMTWYQDLEEFCYRENELEMNDENSDHKVLSAVIEKTVIPKVSGFVELLWDPLSAVQTDNLAHFCKTNVKHNESSKAVQGLINCLLSTMKKAIEDDVFIPLFPKRLLEDRFSPHSRFQERRFWSAVKMFQNVLCWDGFLQEETLQELSLDKLLNRYLLLVILNAEPGPDSVKKCKRVVECLPQSWFRNLESGSSLHRLLNFSKHLLQSIHTLHKLNDRENMKILVSLLLKIKAVDYAEEAISQYNLEELKVVT

Target ORF information:

RefSeq Version	XM_002935987.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935987.5

ORF Insert Sequence:

ATGTTTAGAA AACCGAAGCG AGCGATTCGT GTGAGGAAAG CGGAGTCCAG CGAGGAAGAG 
GAGCGGGAAG AAGAGAGTGA GCAACCCCGG GCCAGGGAGC AGAGGAGGCC AGCCGGCCGC 
GGCTTATTTT GTGGGACTGA GCGAGGGAAG AGGGCCGAAG CGGCGCAGGG AGACGGGGCT 
GGGAGTGATG CGGCGCGGAG AGACGGGGCT GGGAGTGATG CGGCGCGGAG AGACGGGGCT 
GGGAGTGATG CGGCGCGGAG AGACGGGGCT GGGAGTGATG CGGAAGGGGA ACCGGATCCC 
AGTGAAACAG TCGGGACTGC TCCTGTGGAA CCCGAATTCT CTATTCGCTT TACAGCCCCG 
CCCACCCTGC TGAGCTTCGC TGAGGAGAGA GAAGATGCAG ACTTCAAGAT CAAGAAGCCC 
AGTGTCAATG CAGTGGTATT TCAAGTGCAG AAAAAGACAG AAAAGAACTC CATAATTAAG 
TCCTATAAAA AAGAAAGAGC AGCCAAGACA GAAAGTGACA GTGACTCTGA GGTGGAGGGT 
CTGGAAGAAG ATGGAGCAAA GTCCAAGAGT GACAGCAGCT CTGAGCTGGA AGATCAGAGA 
GAAGATGGAG CAAAATCAGA GAGTAACAGT AGCTCAGAGG TGGAGAATAA AGACCCTCAG 
CCACAAGAAA GGTCAGACTC TCCAAACTCT GGAATGGGCA GCTCACCTAC ATCTCCTGAC 
TCTGGCATCA CAGGTGCTAT TCCAGGCGCT AAGGTCATTC GAGCAGCTAG AAGGCGGCGC 
AGGTTGGCCC GAGCTCAAGG TGATTATATA CCCATTGGCA CAAAGCAGGA AATGTCCAGT 
TCCAGCCAGT CAGAAAGTGA CAGTGACATT GATGATCACG AGAGAAGAAT CAAGTTTGCC 
CCGGGAACAA AGACTTTGCG AGAGCAGATG GAAGAGGAAA TGTCATCAAA CAGTGACTCT 
GAGATGCATA AAAGTGAAGA TGAAGAGGAT TTGCAAGACA AATGGGAGGA ACAGCAAATA 
CGGAAAGCTG TCAAATATCA AAAGGGAATG GATGAGGATT TGCCACAAGT GAGAATTCCA 
CCCAAGAGTA AAAAGAGTGT TGAACCAAGG ATTTCTCTGC CACCAGTAAC TGCTGAAGAC 
ATAAAAAAGA AACTGGCTTC ACGGTTGAAT TCCTTTCATG AAGTCCACCG GGCACATGTT 
GCTGAGCGAG AAAAGTATGT TAGTGATCTG GACAGTGCAA AAACCACACT AGAAAAGCTT 
GAAATGTCTT CCAGTGAGCA AACCTATAAG TTTTTCAAGG AGATGAAAAC CTATGTAGAA 
AACTTTGTTG ACTGTGTGAA TGAAAAGATA GCCCAGATCA ATAGGCTTGA ATTAGAGATG 
ATTGAGATTT TTCAGAAACG AGCTGAATCC TTGAATAAGC GCAGGCAAGA TGACCTTCGG 
AATGAGTCTG TAGCTGTTCA AAATATATCT GGAACTAAAG ATGCCAATGG CCAGACTACA 
GACCTGATGA CTGATTGTGA GTTACGAAGA AAGAAAAGAA GGCAAAAACG GAAGGAGTGT 
GGGGACTCAG ATCACTATGA AGGGATGTCT AGTGATGATG AACTGTCCAC TGATGATGAG 
AGGAGTTTCC AGAAAAACAG AGAGAGCATC AGGGCACAGA GTAAAACCAT ATTTGAGGAT 
GTGCATGAAG ATTTCCACCA GATAAAGAAT ATCCTGTCCA GATTTACAGA GTGGAGAGGG 
AGATTTCCTG AGTCCTATTA CGATGCCTAC ATTAGCCTGT GCCTCCACAA ACTACTGAAT 
CCAATAATCC GAGTGCATCT GTTAGACTGG AATCCCTTGG AGGATAAAAA AGATCTGGAA 
GAGATGACAT GGTACCAAGA CTTAGAAGAG TTTTGCTATA GGGAGAATGA GCTGGAGATG 
AATGACGAGA ATTCGGACCA TAAAGTCCTG TCCGCTGTGA TTGAGAAAAC GGTTATTCCA 
AAAGTTTCAG GATTTGTTGA ACTCTTGTGG GATCCACTAT CCGCTGTACA GACTGACAAC 
CTAGCGCATT TCTGCAAAAC AAATGTCAAG CATAATGAGT CCAGTAAAGC AGTGCAGGGC 
CTGATAAATT GTCTACTTTC AACAATGAAG AAAGCCATTG AAGATGATGT GTTTATTCCT 
CTCTTTCCAA AAAGACTTTT GGAAGACAGA TTCTCTCCGC ATTCAAGATT CCAAGAAAGG 
CGTTTCTGGT CTGCTGTGAA AATGTTCCAG AACGTTCTTT GCTGGGATGG ATTCCTCCAA 
GAAGAGACCC TGCAAGAGCT CAGCCTTGAT AAGCTGCTTA ATCGATACCT ACTGCTAGTA 
ATTCTGAATG CAGAGCCAGG ACCAGACAGT GTGAAGAAGT GCAAGAGAGT GGTTGAATGT 
CTCCCTCAGA GCTGGTTTAG GAACCTTGAG AGTGGATCAT CTCTTCATCG CTTACTGAAT 
TTCAGCAAAC ACCTACTGCA GTCTATTCAT ACTCTGCACA AGCTAAATGA CAGAGAAAAC 
ATGAAGATTT TAGTGTCCCT GCTCTTGAAG ATCAAAGCCG TGGACTACGC AGAGGAAGCC 
ATCAGCCAGT ATAACCTGGA AGAACTGAAA GTGGTCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcfc2 ( XM_002935987.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936033.2 Xenopus tropicalis GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.

Price & Availability↑

CloneID	OXa16654
Clone ID Related Accession (Same CDS sequence)	XM_002935987.5 , XM_002935987.4
Accession Version	XM_002935987.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	GC-rich sequence DNA-binding factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935987.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGAA AACCGAAGCG AGCGATTCGT GTGAGGAAAG CGGAGTCCAG CGAGGAAGAG 
GAGCGGGAAG AAGAGAGTGA GCAACCCCGG GCCAGGGAGC AGAGGAGGCC AGCCGGCCGC 
GGCTTATTTT GTGGGACTGA GCGAGGGAAG AGGGCCGAAG CGGCGCAGGG AGACGGGGCT 
GGGAGTGATG CGGCGCGGAG AGACGGGGCT GGGAGTGATG CGGCGCGGAG AGACGGGGCT 
GGGAGTGATG CGGCGCGGAG AGACGGGGCT GGGAGTGATG CGGAAGGGGA ACCGGATCCC 
AGTGAAACAG TCGGGACTGC TCCTGTGGAA CCCGAATTCT CTATTCGCTT TACAGCCCCG 
CCCACCCTGC TGAGCTTCGC TGAGGAGAGA GAAGATGCAG ACTTCAAGAT CAAGAAGCCC 
AGTGTCAATG CAGTGGTATT TCAAGTGCAG AAAAAGACAG AAAAGAACTC CATAATTAAG 
TCCTATAAAA AAGAAAGAGC AGCCAAGACA GAAAGTGACA GTGACTCTGA GGTGGAGGGT 
CTGGAAGAAG ATGGAGCAAA GTCCAAGAGT GACAGCAGCT CTGAGCTGGA AGATCAGAGA 
GAAGATGGAG CAAAATCAGA GAGTAACAGT AGCTCAGAGG TGGAGAATAA AGACCCTCAG 
CCACAAGAAA GGTCAGACTC TCCAAACTCT GGAATGGGCA GCTCACCTAC ATCTCCTGAC 
TCTGGCATCA CAGGTGCTAT TCCAGGCGCT AAGGTCATTC GAGCAGCTAG AAGGCGGCGC 
AGGTTGGCCC GAGCTCAAGG TGATTATATA CCCATTGGCA CAAAGCAGGA AATGTCCAGT 
TCCAGCCAGT CAGAAAGTGA CAGTGACATT GATGATCACG AGAGAAGAAT CAAGTTTGCC 
CCGGGAACAA AGACTTTGCG AGAGCAGATG GAAGAGGAAA TGTCATCAAA CAGTGACTCT 
GAGATGCATA AAAGTGAAGA TGAAGAGGAT TTGCAAGACA AATGGGAGGA ACAGCAAATA 
CGGAAAGCTG TCAAATATCA AAAGGGAATG GATGAGGATT TGCCACAAGT GAGAATTCCA 
CCCAAGAGTA AAAAGAGTGT TGAACCAAGG ATTTCTCTGC CACCAGTAAC TGCTGAAGAC 
ATAAAAAAGA AACTGGCTTC ACGGTTGAAT TCCTTTCATG AAGTCCACCG GGCACATGTT 
GCTGAGCGAG AAAAGTATGT TAGTGATCTG GACAGTGCAA AAACCACACT AGAAAAGCTT 
GAAATGTCTT CCAGTGAGCA AACCTATAAG TTTTTCAAGG AGATGAAAAC CTATGTAGAA 
AACTTTGTTG ACTGTGTGAA TGAAAAGATA GCCCAGATCA ATAGGCTTGA ATTAGAGATG 
ATTGAGATTT TTCAGAAACG AGCTGAATCC TTGAATAAGC GCAGGCAAGA TGACCTTCGG 
AATGAGTCTG TAGCTGTTCA AAATATATCT GGAACTAAAG ATGCCAATGG CCAGACTACA 
GACCTGATGA CTGATTGTGA GTTACGAAGA AAGAAAAGAA GGCAAAAACG GAAGGAGTGT 
GGGGACTCAG ATCACTATGA AGGGATGTCT AGTGATGATG AACTGTCCAC TGATGATGAG 
AGGAGTTTCC AGAAAAACAG AGAGAGCATC AGGGCACAGA GTAAAACCAT ATTTGAGGAT 
GTGCATGAAG ATTTCCACCA GATAAAGAAT ATCCTGTCCA GATTTACAGA GTGGAGAGGG 
AGATTTCCTG AGTCCTATTA CGATGCCTAC ATTAGCCTGT GCCTCCACAA ACTACTGAAT 
CCAATAATCC GAGTGCATCT GTTAGACTGG AATCCCTTGG AGGATAAAAA AGATCTGGAA 
GAGATGACAT GGTACCAAGA CTTAGAAGAG TTTTGCTATA GGGAGAATGA GCTGGAGATG 
AATGACGAGA ATTCGGACCA TAAAGTCCTG TCCGCTGTGA TTGAGAAAAC GGTTATTCCA 
AAAGTTTCAG GATTTGTTGA ACTCTTGTGG GATCCACTAT CCGCTGTACA GACTGACAAC 
CTAGCGCATT TCTGCAAAAC AAATGTCAAG CATAATGAGT CCAGTAAAGC AGTGCAGGGC 
CTGATAAATT GTCTACTTTC AACAATGAAG AAAGCCATTG AAGATGATGT GTTTATTCCT 
CTCTTTCCAA AAAGACTTTT GGAAGACAGA TTCTCTCCGC ATTCAAGATT CCAAGAAAGG 
CGTTTCTGGT CTGCTGTGAA AATGTTCCAG AACGTTCTTT GCTGGGATGG ATTCCTCCAA 
GAAGAGACCC TGCAAGAGCT CAGCCTTGAT AAGCTGCTTA ATCGATACCT ACTGCTAGTA 
ATTCTGAATG CAGAGCCAGG ACCAGACAGT GTGAAGAAGT GCAAGAGAGT GGTTGAATGT 
CTCCCTCAGA GCTGGTTTAG GAACCTTGAG AGTGGATCAT CTCTTCATCG CTTACTGAAT 
TTCAGCAAAC ACCTACTGCA GTCTATTCAT ACTCTGCACA AGCTAAATGA CAGAGAAAAC 
ATGAAGATTT TAGTGTCCCT GCTCTTGAAG ATCAAAGCCG TGGACTACGC AGAGGAAGCC 
ATCAGCCAGT ATAACCTGGA AGAACTGAAA GTGGTCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936033.2
CDS	55..2676
Translation	MFRKPKRAIRVRKAESSEEEEREEESEQPRAREQRRPAGRGLFCGTERGKRAEAAQGDGAGSDAARRDGAGSDAARRDGAGSDAARRDGAGSDAEGEPDPSETVGTAPVEPEFSIRFTAPPTLLSFAEEREDADFKIKKPSVNAVVFQVQKKTEKNSIIKSYKKERAAKTESDSDSEVEGLEEDGAKSKSDSSSELEDQREDGAKSESNSSSEVENKDPQPQERSDSPNSGMGSSPTSPDSGITGAIPGAKVIRAARRRRRLARAQGDYIPIGTKQEMSSSSQSESDSDIDDHERRIKFAPGTKTLREQMEEEMSSNSDSEMHKSEDEEDLQDKWEEQQIRKAVKYQKGMDEDLPQVRIPPKSKKSVEPRISLPPVTAEDIKKKLASRLNSFHEVHRAHVAEREKYVSDLDSAKTTLEKLEMSSSEQTYKFFKEMKTYVENFVDCVNEKIAQINRLELEMIEIFQKRAESLNKRRQDDLRNESVAVQNISGTKDANGQTTDLMTDCELRRKKRRQKRKECGDSDHYEGMSSDDELSTDDERSFQKNRESIRAQSKTIFEDVHEDFHQIKNILSRFTEWRGRFPESYYDAYISLCLHKLLNPIIRVHLLDWNPLEDKKDLEEMTWYQDLEEFCYRENELEMNDENSDHKVLSAVIEKTVIPKVSGFVELLWDPLSAVQTDNLAHFCKTNVKHNESSKAVQGLINCLLSTMKKAIEDDVFIPLFPKRLLEDRFSPHSRFQERRFWSAVKMFQNVLCWDGFLQEETLQELSLDKLLNRYLLLVILNAEPGPDSVKKCKRVVECLPQSWFRNLESGSSLHRLLNFSKHLLQSIHTLHKLNDRENMKILVSLLLKIKAVDYAEEAISQYNLEELKVVT

Target ORF information:

RefSeq Version	XM_002935987.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935987.4

ORF Insert Sequence:

ATGTTTAGAA AACCGAAGCG AGCGATTCGT GTGAGGAAAG CGGAGTCCAG CGAGGAAGAG 
GAGCGGGAAG AAGAGAGTGA GCAACCCCGG GCCAGGGAGC AGAGGAGGCC AGCCGGCCGC 
GGCTTATTTT GTGGGACTGA GCGAGGGAAG AGGGCCGAAG CGGCGCAGGG AGACGGGGCT 
GGGAGTGATG CGGCGCGGAG AGACGGGGCT GGGAGTGATG CGGCGCGGAG AGACGGGGCT 
GGGAGTGATG CGGCGCGGAG AGACGGGGCT GGGAGTGATG CGGAAGGGGA ACCGGATCCC 
AGTGAAACAG TCGGGACTGC TCCTGTGGAA CCCGAATTCT CTATTCGCTT TACAGCCCCG 
CCCACCCTGC TGAGCTTCGC TGAGGAGAGA GAAGATGCAG ACTTCAAGAT CAAGAAGCCC 
AGTGTCAATG CAGTGGTATT TCAAGTGCAG AAAAAGACAG AAAAGAACTC CATAATTAAG 
TCCTATAAAA AAGAAAGAGC AGCCAAGACA GAAAGTGACA GTGACTCTGA GGTGGAGGGT 
CTGGAAGAAG ATGGAGCAAA GTCCAAGAGT GACAGCAGCT CTGAGCTGGA AGATCAGAGA 
GAAGATGGAG CAAAATCAGA GAGTAACAGT AGCTCAGAGG TGGAGAATAA AGACCCTCAG 
CCACAAGAAA GGTCAGACTC TCCAAACTCT GGAATGGGCA GCTCACCTAC ATCTCCTGAC 
TCTGGCATCA CAGGTGCTAT TCCAGGCGCT AAGGTCATTC GAGCAGCTAG AAGGCGGCGC 
AGGTTGGCCC GAGCTCAAGG TGATTATATA CCCATTGGCA CAAAGCAGGA AATGTCCAGT 
TCCAGCCAGT CAGAAAGTGA CAGTGACATT GATGATCACG AGAGAAGAAT CAAGTTTGCC 
CCGGGAACAA AGACTTTGCG AGAGCAGATG GAAGAGGAAA TGTCATCAAA CAGTGACTCT 
GAGATGCATA AAAGTGAAGA TGAAGAGGAT TTGCAAGACA AATGGGAGGA ACAGCAAATA 
CGGAAAGCTG TCAAATATCA AAAGGGAATG GATGAGGATT TGCCACAAGT GAGAATTCCA 
CCCAAGAGTA AAAAGAGTGT TGAACCAAGG ATTTCTCTGC CACCAGTAAC TGCTGAAGAC 
ATAAAAAAGA AACTGGCTTC ACGGTTGAAT TCCTTTCATG AAGTCCACCG GGCACATGTT 
GCTGAGCGAG AAAAGTATGT TAGTGATCTG GACAGTGCAA AAACCACACT AGAAAAGCTT 
GAAATGTCTT CCAGTGAGCA AACCTATAAG TTTTTCAAGG AGATGAAAAC CTATGTAGAA 
AACTTTGTTG ACTGTGTGAA TGAAAAGATA GCCCAGATCA ATAGGCTTGA ATTAGAGATG 
ATTGAGATTT TTCAGAAACG AGCTGAATCC TTGAATAAGC GCAGGCAAGA TGACCTTCGG 
AATGAGTCTG TAGCTGTTCA AAATATATCT GGAACTAAAG ATGCCAATGG CCAGACTACA 
GACCTGATGA CTGATTGTGA GTTACGAAGA AAGAAAAGAA GGCAAAAACG GAAGGAGTGT 
GGGGACTCAG ATCACTATGA AGGGATGTCT AGTGATGATG AACTGTCCAC TGATGATGAG 
AGGAGTTTCC AGAAAAACAG AGAGAGCATC AGGGCACAGA GTAAAACCAT ATTTGAGGAT 
GTGCATGAAG ATTTCCACCA GATAAAGAAT ATCCTGTCCA GATTTACAGA GTGGAGAGGG 
AGATTTCCTG AGTCCTATTA CGATGCCTAC ATTAGCCTGT GCCTCCACAA ACTACTGAAT 
CCAATAATCC GAGTGCATCT GTTAGACTGG AATCCCTTGG AGGATAAAAA AGATCTGGAA 
GAGATGACAT GGTACCAAGA CTTAGAAGAG TTTTGCTATA GGGAGAATGA GCTGGAGATG 
AATGACGAGA ATTCGGACCA TAAAGTCCTG TCCGCTGTGA TTGAGAAAAC GGTTATTCCA 
AAAGTTTCAG GATTTGTTGA ACTCTTGTGG GATCCACTAT CCGCTGTACA GACTGACAAC 
CTAGCGCATT TCTGCAAAAC AAATGTCAAG CATAATGAGT CCAGTAAAGC AGTGCAGGGC 
CTGATAAATT GTCTACTTTC AACAATGAAG AAAGCCATTG AAGATGATGT GTTTATTCCT 
CTCTTTCCAA AAAGACTTTT GGAAGACAGA TTCTCTCCGC ATTCAAGATT CCAAGAAAGG 
CGTTTCTGGT CTGCTGTGAA AATGTTCCAG AACGTTCTTT GCTGGGATGG ATTCCTCCAA 
GAAGAGACCC TGCAAGAGCT CAGCCTTGAT AAGCTGCTTA ATCGATACCT ACTGCTAGTA 
ATTCTGAATG CAGAGCCAGG ACCAGACAGT GTGAAGAAGT GCAAGAGAGT GGTTGAATGT 
CTCCCTCAGA GCTGGTTTAG GAACCTTGAG AGTGGATCAT CTCTTCATCG CTTACTGAAT 
TTCAGCAAAC ACCTACTGCA GTCTATTCAT ACTCTGCACA AGCTAAATGA CAGAGAAAAC 
ATGAAGATTT TAGTGTCCCT GCTCTTGAAG ATCAAAGCCG TGGACTACGC AGAGGAAGCC 
ATCAGCCAGT ATAACCTGGA AGAACTGAAA GTGGTCACGT GA