dna2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dna2
Entrez Gene ID	100170166
Full Name	DNA replication helicase/nuclease 2
Synonyms	dna2l,xdna2
General protein information	Preferred Names DNA replication helicase/nuclease 2 Names DNA replication ATP-dependent helicase/nuclease DNA2 DNA replication helicase 2 homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935970.3	XP_002936016.2	DNA replication ATP-dependent helicase/nuclease DNA2
XM_002935970.4	XP_002936016.2	DNA replication ATP-dependent helicase/nuclease DNA2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DNA2	NP_001006497.1
Bos taurus (cattle)	DNA2	NP_001179731.2
Rattus norvegicus (Norway rat)	Dna2	XP_241671.6
Arabidopsis thaliana (thale cress)	emb2411	NP_001184943.1
Oryza sativa (rice)	Os04g0588200	NP_001053694.2
Canis lupus familiaris (dog)	DNA2	XP_005619489.1
Danio rerio (zebrafish)	dna2	XP_687974.5
Pan troglodytes (chimpanzee)	DNA2	XP_001163213.2
Mus musculus (house mouse)	Dna2	NP_796346.2
Drosophila melanogaster (fruit fly)	CG2990	NP_727386.1
Homo sapiens (human)	DNA2	NP_001073918.2
	DNA2	XP_002808547.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004685	XP_307265.4
Caenorhabditis elegans (roundworm)	dna-2	NP_496515.1
Schizosaccharomyces pombe (fission yeast)	dna2	NP_596499.2
Magnaporthe oryzae (rice blast fungus)	MGG_02491	XP_003709288.1
Xenopus tropicalis (tropical clawed frog)	dna2	XP_002936016.2

Gene Ontology

The following dna2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dna2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa12816	XM_002935970.4 Latest version!	Xenopus tropicalis DNA replication helicase/nuclease 2 (dna2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa12816	XM_002935970.3	Xenopus tropicalis DNA replication helicase/nuclease 2 (dna2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dna2 ( XM_002935970.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936016.2 Xenopus tropicalis DNA replication helicase/nuclease 2 (dna2), mRNA.

Price & Availability↑

CloneID	OXa12816
Clone ID Related Accession (Same CDS sequence)	XM_002935970.3 , XM_002935970.4
Accession Version	XM_002935970.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA replication ATP-dependent helicase/nuclease DNA2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935970.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCCG TGAGTGCTGA GTGTCAGCTG CCCCCGGAGG ATGATTTGTT AGAGATGATG 
ATGGAGCAAA GTTTCACTGA GCCGGAAGAG AAGTCTAAAG ACAAATCAAC CAGAAAAATA 
ATCCCGAAGA CTAAGCTCTG CAGAGGCCTC AACAACAGAT ACTGTGTTCT GAATGTGAAG 
GAAGTATATG CACAAGGAGA GGAGAAACAT CTGACTATTA CAGCATCTCA GGAGGGTGAT 
GATCTAGAGC TCTGCATTCT AAAGGATGAC TGGGTAGCTC TTCATATCAA ACCTGGTGAT 
ATAATCCACT TGGAGGGTAA CTGTGCTTTT GACAACACAT GGACCATAAG CAGAGACACA 
GGATATCTTA TCTTATACCC TGACTTGCTC ATATCTGGTA CTAGCATAGC AAATGGAATT 
AGATGTTTAA GGAGATCGGT ACTGAGTGAA AAATTCAAGG TCTGTGATAA AGGATCCCGT 
CAAATGCTTA TTGGTACAAT GCTTCATGAT ATCTTTCAAA GAGCAACAAC ACGTGGGTTT 
ACTGACAGTG TGCTGCAAGA GCTTGCCCAT CACACAGTTC ATGGGCCAAA GTATCTAAAA 
GAAATGTATC AGTTAAAATT AAATCAAACT GATGTTATGG GAGAAGTACA AGAATATCTT 
CCATCCTTCG CTAAGTGGGC AATTGATTTC ATGACACATC CTCTCAATCA ACACCAGATA 
AATGTTACCA GACCAACAGC TGGAGACCCC ACAGAGGCAA CTAAAGTTTC AGAGTTTCTA 
GACATTGAGG AAAATATTTG GTCCCCAAGA TTTGGTCTAA AGGGCAAAAT TGATGTAACT 
GCTAGGGTAA AAATACACCA GAAGTCCAAA TCTCATTTGA AAATTATGCC TCTTGAGCTA 
AAAACTGGCA AAGAGTCAAA CTCAATAGAA CACAGGAGTC AGGTTGTTTT GTACACTCTT 
TTAAGTCAGG AGAGGCGAGA AGATCCAGAA GCTGGTTTAC TTTTATATCT AAAGACAGGG 
AACATGTACT CTGTACCTGG AAATCGACTT GATAGGAGAG AGTTATTAAA AATAAGGAAT 
GAGTTATCCT ATTACCTGAC AAATGTTGTA CATAAATCAG ACAATGGAAG TAAAGAAATC 
ACTCTAGCTT CTCTGCCAGC CTTGATTGCT GATAGACAAG CCTGCAAGTT TTGTTCACAG 
ATGCGGAACT GTGCCCTTTA TAGCAGATCT GTTGAGCAAC AAATTGAGAA TTGTTACATT 
CCTGCTGAAA TGATTCCAGT TGTTCAGAAG GAAACAGAAC ATTTAAATAA GGATCATTTG 
CAATACTTTA GGCTGTGGTA TCTCATGTGC ACCTTGGAGG GAAATTCCAA GGACTCAAAA 
ATGGGACGCA AAAATATATG GATGATGTCA TCTTCTGAAA GAGAAGAAGA TGGCCAGTGC 
ATTGGGAACC TTATTAGGAC TGGACCTGTA CAAACCATTT CTGATGGGCA ACATCTACAT 
TCCTTTCAGC GTAAATCTGG TACTGTGCCT GCAACAAACT TAATGTCAGG TGATAGAGTT 
GTAGTAAGTG GAGAAGATAA GTTTCTTGCT CTGTCAAGTG GCTACATAAA AGAAGTCAAA 
CATAACAACA TCACTTGCAT TTTGGACAGG AGTCTTGGCA AGCTTCCTGA GGATTTGTTA 
TTTAGGCTTG ATCATGAAGA AGGAGGAGGT GGTTTAGAGT CCCATCTTGG AAACTTATCA 
AGGCTAATGG AGAACTCACC TGTAAGTGAC AAACTGCGGA AACTGATCAT CGACTTTAGC 
AAACCAAATT TTGTTCAACA TTTAAGCTCT GTTCTTCCAT CTGATGCAAA GGATATTGTT 
GCTAACATTT TAAAAGGCCT TAACAAACCT CAGAAGCAGG CAATGAAACG TGTTCTTCTT 
TCTAAAGACT ACACTCTTAT TGTTGGCATG CCAGGGACTG GGAAAACCAC TACAATATGT 
ACCCTGGTGA GAATTTTGTA TGCTTGTGGT TACAGTGTCC TGCTAACCAG TTACACACAC 
TCTGCTGTAG ATAACATTTT ATTGAAGTTA AAAAAATTCC AAGTTGGCTT TCTGCGCTTA 
GGACGAACAC AGAAGCTCCA CCCAGATGTT CAGGAATTCA GCGAAGAAGA AATCTGCAAA 
GCCAAGTCAA TAAAATCTTT AAGTGCTTTG GAAGAACTCT ACAATAGTCA GCCTGTGGTT 
GCAACAACAT GTATGGGTGT GAATCATCCG ATATTTACAC GAAGGCGGTT TGACTTTTGC 
ATTGTTGATG AAGCATCCCA AATTAGTCAG CCAATTTGCC TTGGCCCATT GTTCTTTGCC 
GACAGATTTG TTCTAGTGGG TGACCATCAA CAACTTCCTC CACTAGTGCA AAGTGCAGAA 
GCAAGAGAAC TGGGAATGAG TGAGAGCCTT TTTAAACGGC TGGAAAGGAA TCAAGAGGCT 
GTAGTGCAGT TGACTGTGCA ATATCGAATG AATAGTCAGA TTATGGCACT AAGCAACAAG 
CTGGTGTATG AAGGCAGGCT GGAGTGTGCC TCTGATCGTG TATCTAACGC TGTGGTTAAG 
CTACCTCATA TTAAGACGTT ACTTCTGGAG CTGGAGTTCA GAGAGAGCCA GGAATCAATG 
TGGATTAAAG ATGTATTGGA GCCAAGTAAC CCTGTGTGCT TTCTGAATAC AGAAAAAATT 
CCAGCCCTGG AAACTGAAGA GAAGGGTGGA ATAAGCAACT GGATAGAAGC TAAATTGGTG 
TTCTGTTTGA CAAAGCTGTT TCTAAAGGCT GGCTGCAGAC CCTCAGACAT TGGGATCATA 
GCACCATATC GTCAGCAACT TAAAGTTATC TCTAATTATT TCAACTCCTT GTCAGCGAGT 
GCCGTGGAAG TGAATACAGT AGATAAATAT CAAGGGAGAG ACAAAAGTGT CATCATTGTG 
TCTTTCGTTA GAAGCAATAT TGATGGAAAA CTGGGAGACC TTTTAAAAGA CTGGAGAAGG 
CTAAATGTGG CGCTGACACG GGCCAAACAC AAGCTGATCA TGTTGGGCTG CGTTCCAACC 
CTTAGCCGCT TTCTTTGCCT GGAGCAGCTG ATATGTCATC TAAAATCTAA AAATCTAATT 
TATGATCTTC CAGCCGGCGC CCATGAGCAT ATGCCTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936016.2
CDS	69..3230
Translation	MEPVSAECQLPPEDDLLEMMMEQSFTEPEEKSKDKSTRKIIPKTKLCRGLNNRYCVLNVKEVYAQGEEKHLTITASQEGDDLELCILKDDWVALHIKPGDIIHLEGNCAFDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFKVCDKGSRQMLIGTMLHDIFQRATTRGFTDSVLQELAHHTVHGPKYLKEMYQLKLNQTDVMGEVQEYLPSFAKWAIDFMTHPLNQHQINVTRPTAGDPTEATKVSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKSHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGLLLYLKTGNMYSVPGNRLDRRELLKIRNELSYYLTNVVHKSDNGSKEITLASLPALIADRQACKFCSQMRNCALYSRSVEQQIENCYIPAEMIPVVQKETEHLNKDHLQYFRLWYLMCTLEGNSKDSKMGRKNIWMMSSSEREEDGQCIGNLIRTGPVQTISDGQHLHSFQRKSGTVPATNLMSGDRVVVSGEDKFLALSSGYIKEVKHNNITCILDRSLGKLPEDLLFRLDHEEGGGGLESHLGNLSRLMENSPVSDKLRKLIIDFSKPNFVQHLSSVLPSDAKDIVANILKGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGYSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFADRFVLVGDHQQLPPLVQSAEARELGMSESLFKRLERNQEAVVQLTVQYRMNSQIMALSNKLVYEGRLECASDRVSNAVVKLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFCLTKLFLKAGCRPSDIGIIAPYRQQLKVISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNIDGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLSRFLCLEQLICHLKSKNLIYDLPAGAHEHMPV

Target ORF information:

RefSeq Version	XM_002935970.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA replication helicase/nuclease 2 (dna2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935970.4

ORF Insert Sequence:

ATGGAACCCG TGAGTGCTGA GTGTCAGCTG CCCCCGGAGG ATGATTTGTT AGAGATGATG 
ATGGAGCAAA GTTTCACTGA GCCGGAAGAG AAGTCTAAAG ACAAATCAAC CAGAAAAATA 
ATCCCGAAGA CTAAGCTCTG CAGAGGCCTC AACAACAGAT ACTGTGTTCT GAATGTGAAG 
GAAGTATATG CACAAGGAGA GGAGAAACAT CTGACTATTA CAGCATCTCA GGAGGGTGAT 
GATCTAGAGC TCTGCATTCT AAAGGATGAC TGGGTAGCTC TTCATATCAA ACCTGGTGAT 
ATAATCCACT TGGAGGGTAA CTGTGCTTTT GACAACACAT GGACCATAAG CAGAGACACA 
GGATATCTTA TCTTATACCC TGACTTGCTC ATATCTGGTA CTAGCATAGC AAATGGAATT 
AGATGTTTAA GGAGATCGGT ACTGAGTGAA AAATTCAAGG TCTGTGATAA AGGATCCCGT 
CAAATGCTTA TTGGTACAAT GCTTCATGAT ATCTTTCAAA GAGCAACAAC ACGTGGGTTT 
ACTGACAGTG TGCTGCAAGA GCTTGCCCAT CACACAGTTC ATGGGCCAAA GTATCTAAAA 
GAAATGTATC AGTTAAAATT AAATCAAACT GATGTTATGG GAGAAGTACA AGAATATCTT 
CCATCCTTCG CTAAGTGGGC AATTGATTTC ATGACACATC CTCTCAATCA ACACCAGATA 
AATGTTACCA GACCAACAGC TGGAGACCCC ACAGAGGCAA CTAAAGTTTC AGAGTTTCTA 
GACATTGAGG AAAATATTTG GTCCCCAAGA TTTGGTCTAA AGGGCAAAAT TGATGTAACT 
GCTAGGGTAA AAATACACCA GAAGTCCAAA TCTCATTTGA AAATTATGCC TCTTGAGCTA 
AAAACTGGCA AAGAGTCAAA CTCAATAGAA CACAGGAGTC AGGTTGTTTT GTACACTCTT 
TTAAGTCAGG AGAGGCGAGA AGATCCAGAA GCTGGTTTAC TTTTATATCT AAAGACAGGG 
AACATGTACT CTGTACCTGG AAATCGACTT GATAGGAGAG AGTTATTAAA AATAAGGAAT 
GAGTTATCCT ATTACCTGAC AAATGTTGTA CATAAATCAG ACAATGGAAG TAAAGAAATC 
ACTCTAGCTT CTCTGCCAGC CTTGATTGCT GATAGACAAG CCTGCAAGTT TTGTTCACAG 
ATGCGGAACT GTGCCCTTTA TAGCAGATCT GTTGAGCAAC AAATTGAGAA TTGTTACATT 
CCTGCTGAAA TGATTCCAGT TGTTCAGAAG GAAACAGAAC ATTTAAATAA GGATCATTTG 
CAATACTTTA GGCTGTGGTA TCTCATGTGC ACCTTGGAGG GAAATTCCAA GGACTCAAAA 
ATGGGACGCA AAAATATATG GATGATGTCA TCTTCTGAAA GAGAAGAAGA TGGCCAGTGC 
ATTGGGAACC TTATTAGGAC TGGACCTGTA CAAACCATTT CTGATGGGCA ACATCTACAT 
TCCTTTCAGC GTAAATCTGG TACTGTGCCT GCAACAAACT TAATGTCAGG TGATAGAGTT 
GTAGTAAGTG GAGAAGATAA GTTTCTTGCT CTGTCAAGTG GCTACATAAA AGAAGTCAAA 
CATAACAACA TCACTTGCAT TTTGGACAGG AGTCTTGGCA AGCTTCCTGA GGATTTGTTA 
TTTAGGCTTG ATCATGAAGA AGGAGGAGGT GGTTTAGAGT CCCATCTTGG AAACTTATCA 
AGGCTAATGG AGAACTCACC TGTAAGTGAC AAACTGCGGA AACTGATCAT CGACTTTAGC 
AAACCAAATT TTGTTCAACA TTTAAGCTCT GTTCTTCCAT CTGATGCAAA GGATATTGTT 
GCTAACATTT TAAAAGGCCT TAACAAACCT CAGAAGCAGG CAATGAAACG TGTTCTTCTT 
TCTAAAGACT ACACTCTTAT TGTTGGCATG CCAGGGACTG GGAAAACCAC TACAATATGT 
ACCCTGGTGA GAATTTTGTA TGCTTGTGGT TACAGTGTCC TGCTAACCAG TTACACACAC 
TCTGCTGTAG ATAACATTTT ATTGAAGTTA AAAAAATTCC AAGTTGGCTT TCTGCGCTTA 
GGACGAACAC AGAAGCTCCA CCCAGATGTT CAGGAATTCA GCGAAGAAGA AATCTGCAAA 
GCCAAGTCAA TAAAATCTTT AAGTGCTTTG GAAGAACTCT ACAATAGTCA GCCTGTGGTT 
GCAACAACAT GTATGGGTGT GAATCATCCG ATATTTACAC GAAGGCGGTT TGACTTTTGC 
ATTGTTGATG AAGCATCCCA AATTAGTCAG CCAATTTGCC TTGGCCCATT GTTCTTTGCC 
GACAGATTTG TTCTAGTGGG TGACCATCAA CAACTTCCTC CACTAGTGCA AAGTGCAGAA 
GCAAGAGAAC TGGGAATGAG TGAGAGCCTT TTTAAACGGC TGGAAAGGAA TCAAGAGGCT 
GTAGTGCAGT TGACTGTGCA ATATCGAATG AATAGTCAGA TTATGGCACT AAGCAACAAG 
CTGGTGTATG AAGGCAGGCT GGAGTGTGCC TCTGATCGTG TATCTAACGC TGTGGTTAAG 
CTACCTCATA TTAAGACGTT ACTTCTGGAG CTGGAGTTCA GAGAGAGCCA GGAATCAATG 
TGGATTAAAG ATGTATTGGA GCCAAGTAAC CCTGTGTGCT TTCTGAATAC AGAAAAAATT 
CCAGCCCTGG AAACTGAAGA GAAGGGTGGA ATAAGCAACT GGATAGAAGC TAAATTGGTG 
TTCTGTTTGA CAAAGCTGTT TCTAAAGGCT GGCTGCAGAC CCTCAGACAT TGGGATCATA 
GCACCATATC GTCAGCAACT TAAAGTTATC TCTAATTATT TCAACTCCTT GTCAGCGAGT 
GCCGTGGAAG TGAATACAGT AGATAAATAT CAAGGGAGAG ACAAAAGTGT CATCATTGTG 
TCTTTCGTTA GAAGCAATAT TGATGGAAAA CTGGGAGACC TTTTAAAAGA CTGGAGAAGG 
CTAAATGTGG CGCTGACACG GGCCAAACAC AAGCTGATCA TGTTGGGCTG CGTTCCAACC 
CTTAGCCGCT TTCTTTGCCT GGAGCAGCTG ATATGTCATC TAAAATCTAA AAATCTAATT 
TATGATCTTC CAGCCGGCGC CCATGAGCAT ATGCCTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dna2 ( XM_002935970.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936016.2 Xenopus tropicalis DNA replication helicase/nuclease 2 (dna2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa12816
Clone ID Related Accession (Same CDS sequence)	XM_002935970.3 , XM_002935970.4
Accession Version	XM_002935970.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA replication ATP-dependent helicase/nuclease DNA2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002935970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCCG TGAGTGCTGA GTGTCAGCTG CCCCCGGAGG ATGATTTGTT AGAGATGATG 
ATGGAGCAAA GTTTCACTGA GCCGGAAGAG AAGTCTAAAG ACAAATCAAC CAGAAAAATA 
ATCCCGAAGA CTAAGCTCTG CAGAGGCCTC AACAACAGAT ACTGTGTTCT GAATGTGAAG 
GAAGTATATG CACAAGGAGA GGAGAAACAT CTGACTATTA CAGCATCTCA GGAGGGTGAT 
GATCTAGAGC TCTGCATTCT AAAGGATGAC TGGGTAGCTC TTCATATCAA ACCTGGTGAT 
ATAATCCACT TGGAGGGTAA CTGTGCTTTT GACAACACAT GGACCATAAG CAGAGACACA 
GGATATCTTA TCTTATACCC TGACTTGCTC ATATCTGGTA CTAGCATAGC AAATGGAATT 
AGATGTTTAA GGAGATCGGT ACTGAGTGAA AAATTCAAGG TCTGTGATAA AGGATCCCGT 
CAAATGCTTA TTGGTACAAT GCTTCATGAT ATCTTTCAAA GAGCAACAAC ACGTGGGTTT 
ACTGACAGTG TGCTGCAAGA GCTTGCCCAT CACACAGTTC ATGGGCCAAA GTATCTAAAA 
GAAATGTATC AGTTAAAATT AAATCAAACT GATGTTATGG GAGAAGTACA AGAATATCTT 
CCATCCTTCG CTAAGTGGGC AATTGATTTC ATGACACATC CTCTCAATCA ACACCAGATA 
AATGTTACCA GACCAACAGC TGGAGACCCC ACAGAGGCAA CTAAAGTTTC AGAGTTTCTA 
GACATTGAGG AAAATATTTG GTCCCCAAGA TTTGGTCTAA AGGGCAAAAT TGATGTAACT 
GCTAGGGTAA AAATACACCA GAAGTCCAAA TCTCATTTGA AAATTATGCC TCTTGAGCTA 
AAAACTGGCA AAGAGTCAAA CTCAATAGAA CACAGGAGTC AGGTTGTTTT GTACACTCTT 
TTAAGTCAGG AGAGGCGAGA AGATCCAGAA GCTGGTTTAC TTTTATATCT AAAGACAGGG 
AACATGTACT CTGTACCTGG AAATCGACTT GATAGGAGAG AGTTATTAAA AATAAGGAAT 
GAGTTATCCT ATTACCTGAC AAATGTTGTA CATAAATCAG ACAATGGAAG TAAAGAAATC 
ACTCTAGCTT CTCTGCCAGC CTTGATTGCT GATAGACAAG CCTGCAAGTT TTGTTCACAG 
ATGCGGAACT GTGCCCTTTA TAGCAGATCT GTTGAGCAAC AAATTGAGAA TTGTTACATT 
CCTGCTGAAA TGATTCCAGT TGTTCAGAAG GAAACAGAAC ATTTAAATAA GGATCATTTG 
CAATACTTTA GGCTGTGGTA TCTCATGTGC ACCTTGGAGG GAAATTCCAA GGACTCAAAA 
ATGGGACGCA AAAATATATG GATGATGTCA TCTTCTGAAA GAGAAGAAGA TGGCCAGTGC 
ATTGGGAACC TTATTAGGAC TGGACCTGTA CAAACCATTT CTGATGGGCA ACATCTACAT 
TCCTTTCAGC GTAAATCTGG TACTGTGCCT GCAACAAACT TAATGTCAGG TGATAGAGTT 
GTAGTAAGTG GAGAAGATAA GTTTCTTGCT CTGTCAAGTG GCTACATAAA AGAAGTCAAA 
CATAACAACA TCACTTGCAT TTTGGACAGG AGTCTTGGCA AGCTTCCTGA GGATTTGTTA 
TTTAGGCTTG ATCATGAAGA AGGAGGAGGT GGTTTAGAGT CCCATCTTGG AAACTTATCA 
AGGCTAATGG AGAACTCACC TGTAAGTGAC AAACTGCGGA AACTGATCAT CGACTTTAGC 
AAACCAAATT TTGTTCAACA TTTAAGCTCT GTTCTTCCAT CTGATGCAAA GGATATTGTT 
GCTAACATTT TAAAAGGCCT TAACAAACCT CAGAAGCAGG CAATGAAACG TGTTCTTCTT 
TCTAAAGACT ACACTCTTAT TGTTGGCATG CCAGGGACTG GGAAAACCAC TACAATATGT 
ACCCTGGTGA GAATTTTGTA TGCTTGTGGT TACAGTGTCC TGCTAACCAG TTACACACAC 
TCTGCTGTAG ATAACATTTT ATTGAAGTTA AAAAAATTCC AAGTTGGCTT TCTGCGCTTA 
GGACGAACAC AGAAGCTCCA CCCAGATGTT CAGGAATTCA GCGAAGAAGA AATCTGCAAA 
GCCAAGTCAA TAAAATCTTT AAGTGCTTTG GAAGAACTCT ACAATAGTCA GCCTGTGGTT 
GCAACAACAT GTATGGGTGT GAATCATCCG ATATTTACAC GAAGGCGGTT TGACTTTTGC 
ATTGTTGATG AAGCATCCCA AATTAGTCAG CCAATTTGCC TTGGCCCATT GTTCTTTGCC 
GACAGATTTG TTCTAGTGGG TGACCATCAA CAACTTCCTC CACTAGTGCA AAGTGCAGAA 
GCAAGAGAAC TGGGAATGAG TGAGAGCCTT TTTAAACGGC TGGAAAGGAA TCAAGAGGCT 
GTAGTGCAGT TGACTGTGCA ATATCGAATG AATAGTCAGA TTATGGCACT AAGCAACAAG 
CTGGTGTATG AAGGCAGGCT GGAGTGTGCC TCTGATCGTG TATCTAACGC TGTGGTTAAG 
CTACCTCATA TTAAGACGTT ACTTCTGGAG CTGGAGTTCA GAGAGAGCCA GGAATCAATG 
TGGATTAAAG ATGTATTGGA GCCAAGTAAC CCTGTGTGCT TTCTGAATAC AGAAAAAATT 
CCAGCCCTGG AAACTGAAGA GAAGGGTGGA ATAAGCAACT GGATAGAAGC TAAATTGGTG 
TTCTGTTTGA CAAAGCTGTT TCTAAAGGCT GGCTGCAGAC CCTCAGACAT TGGGATCATA 
GCACCATATC GTCAGCAACT TAAAGTTATC TCTAATTATT TCAACTCCTT GTCAGCGAGT 
GCCGTGGAAG TGAATACAGT AGATAAATAT CAAGGGAGAG ACAAAAGTGT CATCATTGTG 
TCTTTCGTTA GAAGCAATAT TGATGGAAAA CTGGGAGACC TTTTAAAAGA CTGGAGAAGG 
CTAAATGTGG CGCTGACACG GGCCAAACAC AAGCTGATCA TGTTGGGCTG CGTTCCAACC 
CTTAGCCGCT TTCTTTGCCT GGAGCAGCTG ATATGTCATC TAAAATCTAA AAATCTAATT 
TATGATCTTC CAGCCGGCGC CCATGAGCAT ATGCCTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936016.2
CDS	69..3230
Translation	MEPVSAECQLPPEDDLLEMMMEQSFTEPEEKSKDKSTRKIIPKTKLCRGLNNRYCVLNVKEVYAQGEEKHLTITASQEGDDLELCILKDDWVALHIKPGDIIHLEGNCAFDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFKVCDKGSRQMLIGTMLHDIFQRATTRGFTDSVLQELAHHTVHGPKYLKEMYQLKLNQTDVMGEVQEYLPSFAKWAIDFMTHPLNQHQINVTRPTAGDPTEATKVSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKSHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGLLLYLKTGNMYSVPGNRLDRRELLKIRNELSYYLTNVVHKSDNGSKEITLASLPALIADRQACKFCSQMRNCALYSRSVEQQIENCYIPAEMIPVVQKETEHLNKDHLQYFRLWYLMCTLEGNSKDSKMGRKNIWMMSSSEREEDGQCIGNLIRTGPVQTISDGQHLHSFQRKSGTVPATNLMSGDRVVVSGEDKFLALSSGYIKEVKHNNITCILDRSLGKLPEDLLFRLDHEEGGGGLESHLGNLSRLMENSPVSDKLRKLIIDFSKPNFVQHLSSVLPSDAKDIVANILKGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGYSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFADRFVLVGDHQQLPPLVQSAEARELGMSESLFKRLERNQEAVVQLTVQYRMNSQIMALSNKLVYEGRLECASDRVSNAVVKLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFCLTKLFLKAGCRPSDIGIIAPYRQQLKVISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNIDGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLSRFLCLEQLICHLKSKNLIYDLPAGAHEHMPV

Target ORF information:

RefSeq Version	XM_002935970.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA replication helicase/nuclease 2 (dna2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935970.3

ORF Insert Sequence:

ATGGAACCCG TGAGTGCTGA GTGTCAGCTG CCCCCGGAGG ATGATTTGTT AGAGATGATG 
ATGGAGCAAA GTTTCACTGA GCCGGAAGAG AAGTCTAAAG ACAAATCAAC CAGAAAAATA 
ATCCCGAAGA CTAAGCTCTG CAGAGGCCTC AACAACAGAT ACTGTGTTCT GAATGTGAAG 
GAAGTATATG CACAAGGAGA GGAGAAACAT CTGACTATTA CAGCATCTCA GGAGGGTGAT 
GATCTAGAGC TCTGCATTCT AAAGGATGAC TGGGTAGCTC TTCATATCAA ACCTGGTGAT 
ATAATCCACT TGGAGGGTAA CTGTGCTTTT GACAACACAT GGACCATAAG CAGAGACACA 
GGATATCTTA TCTTATACCC TGACTTGCTC ATATCTGGTA CTAGCATAGC AAATGGAATT 
AGATGTTTAA GGAGATCGGT ACTGAGTGAA AAATTCAAGG TCTGTGATAA AGGATCCCGT 
CAAATGCTTA TTGGTACAAT GCTTCATGAT ATCTTTCAAA GAGCAACAAC ACGTGGGTTT 
ACTGACAGTG TGCTGCAAGA GCTTGCCCAT CACACAGTTC ATGGGCCAAA GTATCTAAAA 
GAAATGTATC AGTTAAAATT AAATCAAACT GATGTTATGG GAGAAGTACA AGAATATCTT 
CCATCCTTCG CTAAGTGGGC AATTGATTTC ATGACACATC CTCTCAATCA ACACCAGATA 
AATGTTACCA GACCAACAGC TGGAGACCCC ACAGAGGCAA CTAAAGTTTC AGAGTTTCTA 
GACATTGAGG AAAATATTTG GTCCCCAAGA TTTGGTCTAA AGGGCAAAAT TGATGTAACT 
GCTAGGGTAA AAATACACCA GAAGTCCAAA TCTCATTTGA AAATTATGCC TCTTGAGCTA 
AAAACTGGCA AAGAGTCAAA CTCAATAGAA CACAGGAGTC AGGTTGTTTT GTACACTCTT 
TTAAGTCAGG AGAGGCGAGA AGATCCAGAA GCTGGTTTAC TTTTATATCT AAAGACAGGG 
AACATGTACT CTGTACCTGG AAATCGACTT GATAGGAGAG AGTTATTAAA AATAAGGAAT 
GAGTTATCCT ATTACCTGAC AAATGTTGTA CATAAATCAG ACAATGGAAG TAAAGAAATC 
ACTCTAGCTT CTCTGCCAGC CTTGATTGCT GATAGACAAG CCTGCAAGTT TTGTTCACAG 
ATGCGGAACT GTGCCCTTTA TAGCAGATCT GTTGAGCAAC AAATTGAGAA TTGTTACATT 
CCTGCTGAAA TGATTCCAGT TGTTCAGAAG GAAACAGAAC ATTTAAATAA GGATCATTTG 
CAATACTTTA GGCTGTGGTA TCTCATGTGC ACCTTGGAGG GAAATTCCAA GGACTCAAAA 
ATGGGACGCA AAAATATATG GATGATGTCA TCTTCTGAAA GAGAAGAAGA TGGCCAGTGC 
ATTGGGAACC TTATTAGGAC TGGACCTGTA CAAACCATTT CTGATGGGCA ACATCTACAT 
TCCTTTCAGC GTAAATCTGG TACTGTGCCT GCAACAAACT TAATGTCAGG TGATAGAGTT 
GTAGTAAGTG GAGAAGATAA GTTTCTTGCT CTGTCAAGTG GCTACATAAA AGAAGTCAAA 
CATAACAACA TCACTTGCAT TTTGGACAGG AGTCTTGGCA AGCTTCCTGA GGATTTGTTA 
TTTAGGCTTG ATCATGAAGA AGGAGGAGGT GGTTTAGAGT CCCATCTTGG AAACTTATCA 
AGGCTAATGG AGAACTCACC TGTAAGTGAC AAACTGCGGA AACTGATCAT CGACTTTAGC 
AAACCAAATT TTGTTCAACA TTTAAGCTCT GTTCTTCCAT CTGATGCAAA GGATATTGTT 
GCTAACATTT TAAAAGGCCT TAACAAACCT CAGAAGCAGG CAATGAAACG TGTTCTTCTT 
TCTAAAGACT ACACTCTTAT TGTTGGCATG CCAGGGACTG GGAAAACCAC TACAATATGT 
ACCCTGGTGA GAATTTTGTA TGCTTGTGGT TACAGTGTCC TGCTAACCAG TTACACACAC 
TCTGCTGTAG ATAACATTTT ATTGAAGTTA AAAAAATTCC AAGTTGGCTT TCTGCGCTTA 
GGACGAACAC AGAAGCTCCA CCCAGATGTT CAGGAATTCA GCGAAGAAGA AATCTGCAAA 
GCCAAGTCAA TAAAATCTTT AAGTGCTTTG GAAGAACTCT ACAATAGTCA GCCTGTGGTT 
GCAACAACAT GTATGGGTGT GAATCATCCG ATATTTACAC GAAGGCGGTT TGACTTTTGC 
ATTGTTGATG AAGCATCCCA AATTAGTCAG CCAATTTGCC TTGGCCCATT GTTCTTTGCC 
GACAGATTTG TTCTAGTGGG TGACCATCAA CAACTTCCTC CACTAGTGCA AAGTGCAGAA 
GCAAGAGAAC TGGGAATGAG TGAGAGCCTT TTTAAACGGC TGGAAAGGAA TCAAGAGGCT 
GTAGTGCAGT TGACTGTGCA ATATCGAATG AATAGTCAGA TTATGGCACT AAGCAACAAG 
CTGGTGTATG AAGGCAGGCT GGAGTGTGCC TCTGATCGTG TATCTAACGC TGTGGTTAAG 
CTACCTCATA TTAAGACGTT ACTTCTGGAG CTGGAGTTCA GAGAGAGCCA GGAATCAATG 
TGGATTAAAG ATGTATTGGA GCCAAGTAAC CCTGTGTGCT TTCTGAATAC AGAAAAAATT 
CCAGCCCTGG AAACTGAAGA GAAGGGTGGA ATAAGCAACT GGATAGAAGC TAAATTGGTG 
TTCTGTTTGA CAAAGCTGTT TCTAAAGGCT GGCTGCAGAC CCTCAGACAT TGGGATCATA 
GCACCATATC GTCAGCAACT TAAAGTTATC TCTAATTATT TCAACTCCTT GTCAGCGAGT 
GCCGTGGAAG TGAATACAGT AGATAAATAT CAAGGGAGAG ACAAAAGTGT CATCATTGTG 
TCTTTCGTTA GAAGCAATAT TGATGGAAAA CTGGGAGACC TTTTAAAAGA CTGGAGAAGG 
CTAAATGTGG CGCTGACACG GGCCAAACAC AAGCTGATCA TGTTGGGCTG CGTTCCAACC 
CTTAGCCGCT TTCTTTGCCT GGAGCAGCTG ATATGTCATC TAAAATCTAA AAATCTAATT 
TATGATCTTC CAGCCGGCGC CCATGAGCAT ATGCCTGTAT GA