lct.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	lct.2
Entrez Gene ID	100145766
Full Name	lactase, gene 2
Synonyms	lac,lactase,lph,lph1
General protein information	Preferred Names lactase, gene 2 Names lactase-phlorizin hydrolase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012970934.2	XP_012826388.2	lactase-phlorizin hydrolase
XM_012970934.1	XP_012826388.1	lactase-phlorizin hydrolase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LCT	XP_003954409.1
	LCT	XP_001096426.2
Canis lupus familiaris (dog)	LCT	XP_005632060.1
Mus musculus (house mouse)	Lct	NP_001074547.1
Danio rerio (zebrafish)	lct	XP_001336765.5
Xenopus tropicalis (tropical clawed frog)	LOC101732850	XP_004917683.1
Rattus norvegicus (Norway rat)	Lct	NP_446293.1
Gallus gallus (chicken)	LCT	NP_001104816.1
Xenopus tropicalis (tropical clawed frog)	lct.2	XP_002934014.2
Homo sapiens (human)	LCT	NP_002290.2
Bos taurus (cattle)	LCT	NP_001192716.1

Gene Ontology

The following lct.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lct.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa67164	XM_012970934.2 Latest version!	Xenopus tropicalis lactase, gene 2 (lct.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa32043	XM_012970934.1 Latest version!	Xenopus tropicalis lactase, gene 2 (lct.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lct.2 ( XM_012970934.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826388.2 Xenopus tropicalis lactase, gene 2 (lct.2), mRNA.

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CloneID	OXa67164
Clone ID Related Accession (Same CDS sequence)	XM_012970934.2
Accession Version	XM_012970934.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lactase-phlorizin hydrolase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG CCTACAGGGT GCTACTCGCA CTGCTGCTCT GCTCTCTGTA CCAGGGAGCC 
CATAACCTGA CACCCAATGA GAAGCTTGTG GCCCTTGCTG GACCCATAAC AAGCAGTGTA 
TTGGGGCCAC TGAATGAGGG AAATGGAGAC ATTGGGTGCC ATGATGCCCT TTTGCAGAAG 
CTTAGAGACC GGCTGCCAAC GCTGAGCCAA TATGGCGCCA CCCATTATAA GCTGAACCTA 
CAGTGGGCTG CCCTGTTCCA CAAGGGGTAT GATCAGACAG GGCAAGTCCA GTGCTACCGG 
AACCTGCTCC AACTGCTGGT CAGTGAGAAC ATGAAGCCGG TGGTCATTCT GCAAGGAGAG 
CGCAGCAGTG GCCTCTTGCT TTCCGCTGCC AGTAGCCAAG CGGCCAGTGA CTTGGCCAAT 
GAGTATGTGG CCCATGCTGA CTTTGCCTTC AATGTGTTCG GTGATTTGGT TCAGACCTGG 
ATCACCTTTG ATGTTCCCAC AGGACAGAGC GGGGAGGCAT CTCCTGGGGG CCTGAAGGCC 
CTGCTCCGTG CCCACCAGAG AACCCATGAC TTGTACAGGG GCAAATACTC TTCCAAAGGT 
GGGAAACTGT CTGTTGCCAT GGAACCGGCC TTACTGGAGG CATCTCTGTC CCTTCAGCCA 
CGTCACCGGG AGTTTGTTGA TTTTGTTGCT CTGAAGATAA ACTACAGTTG CAAGAATGGA 
ATCAGTTTGG AGAAACCTCA TTGGGAACAG AGTATCTGGA AGGGGCTGCA GGTTCTGGTT 
TTCCAGTTGA AGCTCGGGGG CTGCGCTTCT CTATCAGATG AAAGTAGATA TCTCCCGACC 
TACCAACTCG CTGCAGGTCT GAGCAGCGCC GGGGCAGTGT TTCTGGGGTA CGACATCAGC 
GCCTTCATAG ACTATTCGCC CATAACTAAG GAAAGGGTGG AACAAGGTGC AGAGAAGTCC 
CTCTTTGCCC CTCTAATTGC CCCTCGTTCC TCCTACACTA CAGTCTGGGA CCGATTTGCT 
AACCAGGCCC CGGCCGAGAG AGACTCCTTC CTGCAGGACG TGTTTCCCAG TGGGTTCTGG 
TGGGGCTCAT CCACTGCTGC TTTCAGAGTG GAAGGGGGTT GGGCAGAAGG GGGGAAAGGA 
GAAACCATCT GGGACAGGTT TGGCCATCAA AACCTGGCAG CACAGAATGC CACCGCCAAC 
GTGGCCTCCG ACAGCTACCG GAAAACTGAC TACGACGTCT ATCTTCTCAA GGGCCTGCAG 
TCCAACGTGT ACAAGTTCTC CATCGCCTGG TCCAGGATCT TCCCAAATGG GCTCAAATCC 
GAGGGGAGTA GAAAGGGGGT GGAATACTAC AATACATTAA TTGACAGCTT AAGAGCCGCA 
GGGGTGGAAC CAATGGTTAC TTTATTCCAC TGGGATCTGC CCCAACCTCT GCAGGACCTT 
GGGGGCTGGA CAAACGAGAA CACGGTAGAT GCCTTTGTAG ACTACGCCGA TTTCTGTTTC 
AGCCATTTTG GAGACCGTGT CAAACTCTGG ATCACGTTTC ATGAGCCGTG GGTCGTCAGC 
TACGCTGGTT ATGGAACTGG GGAACATGCC CCAGGGATTA AGGACCCTGG AAATGCTTCT 
TACAAGGTGG CCCACAACAT CATCAAAGCT CACGCAAAAG CCTGGCATTT GTACGACGGC 
CAATACCGTG CCCATCAACA GGGCAAAGTG GGAATCTCTC TGAACTCAGA CTGGGCGGAG 
CCAGCATCCC CGGCCAATCC TGCTGATGTT GAGGCGGCTG AGCGATACCT TCAGTTCATG 
CTGGGCTGGT TTGCACACCC TATACTGGTC GATGGGGACT ACCCAGCCGT TCTCAAAACC 
CAAATACAGA AGAAGAACCA GCAGTGCCCT GGAACAGTCT CCCAGTTGCC CACCTTCACC 
GAGGTGGAAA AATCCAGCAT CCATGGAACT GCAGACTTCC TGGGAATTTC CCATTATACC 
TCCAGGCTGG TCAATGCATC TGTTAGTGCA GCCTGTGTCT CCGGATATAA CAACATTGGG 
GACTTTGAAC CCTACGTGGA CCCTTCATGG CCGGGGACCT CCTCACCGTG GATCTCAGTG 
GTGCCTTGGG GTATCAGGAG GCTGCTGAAC TTTGTGAAAG AGGAGTATGC AACAGGCAGT 
CTGCCATTAT ACATCACGGG GAATGGGATG CCGACGGCAC ACAATGTAGA GATGTACAAT 
GATCCAACCA GAGTGGACTA TTTAAAGGCC TACATTAACG AGGTCCTGAA AGCTGTCAAG 
ACTGATAATG TGCCAGTTGG AGCTTATATA GTCCGTTCCC TCCTGGATGG ATTTGAAGGC 
CCCCAAGGTT ACAGCCAGAG GTTTGGGTTA CACCACGTGG ATTTTGAGAA CGGCAACAGG 
CAGAGGACCC CCAAAGAATC AGCTTATTTC TTCCACAGAA TCATTGAAAA TAACGGCTTC 
CCCGTGACGA AGAAGACCAA CGTGCCACCG AATTCAAGAT GGCCGTTAGC AAAGAAGTTA 
CCTGCTCTGC CTTCTTCTGA GGTTCCTTCA AAGGCCCAAG TCGTATGGGA AACATTCTCA 
GGGCAAACTG ATTTTGAAAG GGATATGTAT TTCTATGGCA CCTTTCCAAG CGACTTCCAC 
TGGGGGGTTT CAACCTCGGC CTATCAGATA GAAGGTGGGT GGAACGCTGA TGGGAAGGGG 
CCGAGTACAT GGGACACCTA CACGCATATT CCTGGGAATA TTTATAATAA TGACAATGGG 
GACGTTGCCT GTGACAGCTA TAACCAAGCA GACGCAGACG TGTACATGCT CAGGGCCTTG 
GGAGTGACCA GCTATAGGTT CTCCTTGTCT TGGTCTCGGA TCTTCCCAAC TGGCACCGGC 
ACCCCCAATG CAAAAGGGGT GGATTATTAC AACGGACTTA TCAACAAACT TGTAGAAAAC 
CACATTGCCC CGATGGTGAC ACTTTACCAC TTTGACCTTC CCCAGGCTCT TCAGGATATA 
GGTGGTTGGG AGAGCGATGC CGTGCTTGAC GCATTTCACA GCTATGCCGA CTATTGCTTT 
AAGACCTTTG GAGACCGGGT CAAGTTCTGG ATGACGTTTA ACCAACCCCA CACTATTGTG 
ACAGCTGGGT ATGGCCTGGG GTTGATACCA CCTGGACTGA AAGACGACCC AGGATCTGCT 
CCTTATAGAG TAGCCCATAA TCTTTTGAAA GTCCATGCCA GGGTTTACCA TACCTACGAC 
CAACAGTATC GGGCTAGTCA AGGTGGCGTT ATCTCTATAA GTCTCAACAC AGAATGGGCA 
GAACCCAAAA ACCCCAAAGA CCCAAGAGAC GTGACAGCAG CTGACAGATA CTTACAGTTT 
TCACTCGGTT GGTTTGCCCA CCCCATATTC AAAAATGGTG ACTACCCAGA TGCCATGAAG 
TGGCAAGTGG CCAACAAAAG TGACTTACAG AACCTAAAAT CTTCCAGGCT GCCAAGCTTC 
ACGGAGGAGG AGAAGGCCTA CATCCAAGGA ACGGCTGATG TCTTCTGCAT TAACATCTAC 
ACTACGAAAA TAGTTCAATA TAAAACAATC TCCCTGAACC CTCCTTCTTT TGAAAGAGAC 
AGGGATACGG TGGAAGAGGT CAACCCACAG TGGCCAACAA TACCCTTCAT AAACAGCAGA 
GCAGTGGCAT GGGGGTTACG CCGGCTTCTT AACTGGGTGA AGGAGGAGTA TGGGAACGTT 
CCCACCTATG TCACGGAGAA TGGAGATGCA ACAGACGACG TCCCACCAGA TTATGATGAC 
ACCGCACGGA TCTTCTACTA CAAAACCTAC ATTGATGAGG CCTTGAAAGC TTACAGCTTG 
GATGGTGTAA ACCTAAAAGG CTATGCCCCG TGGTCTCTCA TGGACTCATT TGAATGGAAA 
TCAGGATACA GAGTGAGGTT CGGCCTGCAC TACGTGGACT TCAAAAACCC AAGCCGCCCA 
AGGACAGCAA AGCACTCTGC CATCTACTAC TCTCAAGTCA TACGCAAGAA CGGGATCCCA 
CAGGGCAGGG AGGATGAGTT CTTCTATGGG GAGTTCCCCA AGGACTTTGC ATGGAGTGTG 
GCCTCTGCAG CCTTCCAGAT TGAAGGGGGC TGGAGAGCAG ATGGGAAAGG TCTGAGCATC 
TGGGACCAGT TTACACACTC GCCATCCAGA ATCGAAGACG ACAGCAATGG GGACGTGGCC 
TGCAATAGCT ACAACATGAT GGAGAGGGAC GTGGAGATGC TGAGGAACCT CAAAGTCTCC 
CATTACCGAT TTTCAATATC GTGGCCAAGA GTCCTTCCAG ACGGGACCGT GAAGTCTTTC 
AATCAAGCCG GTCTGGATTA TTACATCCGC CTGATAGATG CTCTTCTGGC TGCCAATATA 
GAACCCCAGG TAACTTTGTA TCACTGGGAT CTTCCACAAG AGCTACAGAA CATGGGCGGT 
TGGGAAAATG AGACCATGGT GCAATGGTTT AAAGAGTACG CAGACTTGAT ATTCCAAAAA 
CTGGGAGACA AGGTGAAGAT GTGGATAACG TTCAATGAAC CGTACATTAT TGCCCTCCTT 
GGCTACGGCT ATGGCAACTT TGCCCCAGGT GTTAATGTAA GAATTGGGAC CGCCCCCTAC 
GTGGTGGGAC ACAACATTAT CAAGGCCCAC GCAGAGGCGT GGCATCTGTA CAATGATAAG 
TACAGGGCGG CACAAGGGGG CCTCATCTCC ATTACTGTCA ACTCCGATTG GGCAGAGCCA 
AGGAACCCCT ACAAGCAGGA GGATGTGGAG GCTGCTAGAA GATACTTGTC GTTTTTTTGC 
GGTTGGTTCG CTGATCCCAT TTTCCATGGA GACTACAACG AAGTCATGAA GACGAGGATC 
CTGGAGAGGA GTTTGGGTCA GGGGCTGACT ACATCCCGAC TCCCAGAATT CACGGAGAGC 
GAGAAGCAAA GAATTAAGGG AACTCATGAT TTCTTTGGGC TCAATCATTA CACCACGGTG 
CTGGCAGCGC CCCTGGACTT CCCAGAAGGA GACCCGACGT ATGACGCAGA CAGAGGTACC 
GCTGTGATGA GTGATCGGAC CTGGCTCGGT TCCGGCTCTA ACTGGCTGAG GGTCACCCCG 
TTTGGCCTAA GGAGGCTGCT CAACTGGATA AAGGAAACGT ACAACAACCC CCCCATTTAT 
GTGACAGAAA ACGGGATTTC GGAACGCGGG ACCAATCTGA AGGACGTATG GAGGGAACAT 
TATTATAAAT ATTATATCAA TGAGGCGCTG AAAGCTGTCA AATACGATGG AGCCGACCTC 
AGGGGTTACG CAGCCTGGAG CCTGATGGAT AACTTCGAGT GGGCAGCAGG ATACACGGAA 
AGGTTCGGAC TGTATTACGT CAACTTCACG GACCCCGGGG TCCCGCGTAT CCCTAAGGAT 
TCTGTAAAGT ACTACAGATC TATCATTCAG TGCAATGGTT TCCCAGACCC CAAAGATGGC 
ACCCCCCCTT GTCTTCAGCC TGAACCCGAA GGCACAGCTA AAACAACTGA CCAATCACAC 
GTCACTACTG CCCAACCAAT ACAAGGAGAT GAAGGAACGG TTTCCTTCCT GGGACTGGGA 
ATCTCCGCTG CGGACGCTCA GATCGCTCTG TACGTGGAAT TCGCGCTCAT GATCGCTGGG 
GTTCTAGGGA TTATACTATT TGGTGTTTTA TACAGCAAGT TGGCCAAAAA ATTTAAAAAG 
TCAGTAACAT CATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826388.2
CDS	25..5742
Translation	MMGAYRVLLALLLCSLYQGAHNLTPNEKLVALAGPITSSVLGPLNEGNGDIGCHDALLQKLRDRLPTLSQYGATHYKLNLQWAALFHKGYDQTGQVQCYRNLLQLLVSENMKPVVILQGERSSGLLLSAASSQAASDLANEYVAHADFAFNVFGDLVQTWITFDVPTGQSGEASPGGLKALLRAHQRTHDLYRGKYSSKGGKLSVAMEPALLEASLSLQPRHREFVDFVALKINYSCKNGISLEKPHWEQSIWKGLQVLVFQLKLGGCASLSDESRYLPTYQLAAGLSSAGAVFLGYDISAFIDYSPITKERVEQGAEKSLFAPLIAPRSSYTTVWDRFANQAPAERDSFLQDVFPSGFWWGSSTAAFRVEGGWAEGGKGETIWDRFGHQNLAAQNATANVASDSYRKTDYDVYLLKGLQSNVYKFSIAWSRIFPNGLKSEGSRKGVEYYNTLIDSLRAAGVEPMVTLFHWDLPQPLQDLGGWTNENTVDAFVDYADFCFSHFGDRVKLWITFHEPWVVSYAGYGTGEHAPGIKDPGNASYKVAHNIIKAHAKAWHLYDGQYRAHQQGKVGISLNSDWAEPASPANPADVEAAERYLQFMLGWFAHPILVDGDYPAVLKTQIQKKNQQCPGTVSQLPTFTEVEKSSIHGTADFLGISHYTSRLVNASVSAACVSGYNNIGDFEPYVDPSWPGTSSPWISVVPWGIRRLLNFVKEEYATGSLPLYITGNGMPTAHNVEMYNDPTRVDYLKAYINEVLKAVKTDNVPVGAYIVRSLLDGFEGPQGYSQRFGLHHVDFENGNRQRTPKESAYFFHRIIENNGFPVTKKTNVPPNSRWPLAKKLPALPSSEVPSKAQVVWETFSGQTDFERDMYFYGTFPSDFHWGVSTSAYQIEGGWNADGKGPSTWDTYTHIPGNIYNNDNGDVACDSYNQADADVYMLRALGVTSYRFSLSWSRIFPTGTGTPNAKGVDYYNGLINKLVENHIAPMVTLYHFDLPQALQDIGGWESDAVLDAFHSYADYCFKTFGDRVKFWMTFNQPHTIVTAGYGLGLIPPGLKDDPGSAPYRVAHNLLKVHARVYHTYDQQYRASQGGVISISLNTEWAEPKNPKDPRDVTAADRYLQFSLGWFAHPIFKNGDYPDAMKWQVANKSDLQNLKSSRLPSFTEEEKAYIQGTADVFCINIYTTKIVQYKTISLNPPSFERDRDTVEEVNPQWPTIPFINSRAVAWGLRRLLNWVKEEYGNVPTYVTENGDATDDVPPDYDDTARIFYYKTYIDEALKAYSLDGVNLKGYAPWSLMDSFEWKSGYRVRFGLHYVDFKNPSRPRTAKHSAIYYSQVIRKNGIPQGREDEFFYGEFPKDFAWSVASAAFQIEGGWRADGKGLSIWDQFTHSPSRIEDDSNGDVACNSYNMMERDVEMLRNLKVSHYRFSISWPRVLPDGTVKSFNQAGLDYYIRLIDALLAANIEPQVTLYHWDLPQELQNMGGWENETMVQWFKEYADLIFQKLGDKVKMWITFNEPYIIALLGYGYGNFAPGVNVRIGTAPYVVGHNIIKAHAEAWHLYNDKYRAAQGGLISITVNSDWAEPRNPYKQEDVEAARRYLSFFCGWFADPIFHGDYNEVMKTRILERSLGQGLTTSRLPEFTESEKQRIKGTHDFFGLNHYTTVLAAPLDFPEGDPTYDADRGTAVMSDRTWLGSGSNWLRVTPFGLRRLLNWIKETYNNPPIYVTENGISERGTNLKDVWREHYYKYYINEALKAVKYDGADLRGYAAWSLMDNFEWAAGYTERFGLYYVNFTDPGVPRIPKDSVKYYRSIIQCNGFPDPKDGTPPCLQPEPEGTAKTTDQSHVTTAQPIQGDEGTVSFLGLGISAADAQIALYVEFALMIAGVLGIILFGVLYSKLAKKFKKSVTSF

Target ORF information:

RefSeq Version	XM_012970934.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lactase, gene 2 (lct.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970934.2

ORF Insert Sequence:

ATGATGGGTG CCTACAGGGT GCTACTCGCA CTGCTGCTCT GCTCTCTGTA CCAGGGAGCC 
CATAACCTGA CACCCAATGA GAAGCTTGTG GCCCTTGCTG GACCCATAAC AAGCAGTGTA 
TTGGGGCCAC TGAATGAGGG AAATGGAGAC ATTGGGTGCC ATGATGCCCT TTTGCAGAAG 
CTTAGAGACC GGCTGCCAAC GCTGAGCCAA TATGGCGCCA CCCATTATAA GCTGAACCTA 
CAGTGGGCTG CCCTGTTCCA CAAGGGGTAT GATCAGACAG GGCAAGTCCA GTGCTACCGG 
AACCTGCTCC AACTGCTGGT CAGTGAGAAC ATGAAGCCGG TGGTCATTCT GCAAGGAGAG 
CGCAGCAGTG GCCTCTTGCT TTCCGCTGCC AGTAGCCAAG CGGCCAGTGA CTTGGCCAAT 
GAGTATGTGG CCCATGCTGA CTTTGCCTTC AATGTGTTCG GTGATTTGGT TCAGACCTGG 
ATCACCTTTG ATGTTCCCAC AGGACAGAGC GGGGAGGCAT CTCCTGGGGG CCTGAAGGCC 
CTGCTCCGTG CCCACCAGAG AACCCATGAC TTGTACAGGG GCAAATACTC TTCCAAAGGT 
GGGAAACTGT CTGTTGCCAT GGAACCGGCC TTACTGGAGG CATCTCTGTC CCTTCAGCCA 
CGTCACCGGG AGTTTGTTGA TTTTGTTGCT CTGAAGATAA ACTACAGTTG CAAGAATGGA 
ATCAGTTTGG AGAAACCTCA TTGGGAACAG AGTATCTGGA AGGGGCTGCA GGTTCTGGTT 
TTCCAGTTGA AGCTCGGGGG CTGCGCTTCT CTATCAGATG AAAGTAGATA TCTCCCGACC 
TACCAACTCG CTGCAGGTCT GAGCAGCGCC GGGGCAGTGT TTCTGGGGTA CGACATCAGC 
GCCTTCATAG ACTATTCGCC CATAACTAAG GAAAGGGTGG AACAAGGTGC AGAGAAGTCC 
CTCTTTGCCC CTCTAATTGC CCCTCGTTCC TCCTACACTA CAGTCTGGGA CCGATTTGCT 
AACCAGGCCC CGGCCGAGAG AGACTCCTTC CTGCAGGACG TGTTTCCCAG TGGGTTCTGG 
TGGGGCTCAT CCACTGCTGC TTTCAGAGTG GAAGGGGGTT GGGCAGAAGG GGGGAAAGGA 
GAAACCATCT GGGACAGGTT TGGCCATCAA AACCTGGCAG CACAGAATGC CACCGCCAAC 
GTGGCCTCCG ACAGCTACCG GAAAACTGAC TACGACGTCT ATCTTCTCAA GGGCCTGCAG 
TCCAACGTGT ACAAGTTCTC CATCGCCTGG TCCAGGATCT TCCCAAATGG GCTCAAATCC 
GAGGGGAGTA GAAAGGGGGT GGAATACTAC AATACATTAA TTGACAGCTT AAGAGCCGCA 
GGGGTGGAAC CAATGGTTAC TTTATTCCAC TGGGATCTGC CCCAACCTCT GCAGGACCTT 
GGGGGCTGGA CAAACGAGAA CACGGTAGAT GCCTTTGTAG ACTACGCCGA TTTCTGTTTC 
AGCCATTTTG GAGACCGTGT CAAACTCTGG ATCACGTTTC ATGAGCCGTG GGTCGTCAGC 
TACGCTGGTT ATGGAACTGG GGAACATGCC CCAGGGATTA AGGACCCTGG AAATGCTTCT 
TACAAGGTGG CCCACAACAT CATCAAAGCT CACGCAAAAG CCTGGCATTT GTACGACGGC 
CAATACCGTG CCCATCAACA GGGCAAAGTG GGAATCTCTC TGAACTCAGA CTGGGCGGAG 
CCAGCATCCC CGGCCAATCC TGCTGATGTT GAGGCGGCTG AGCGATACCT TCAGTTCATG 
CTGGGCTGGT TTGCACACCC TATACTGGTC GATGGGGACT ACCCAGCCGT TCTCAAAACC 
CAAATACAGA AGAAGAACCA GCAGTGCCCT GGAACAGTCT CCCAGTTGCC CACCTTCACC 
GAGGTGGAAA AATCCAGCAT CCATGGAACT GCAGACTTCC TGGGAATTTC CCATTATACC 
TCCAGGCTGG TCAATGCATC TGTTAGTGCA GCCTGTGTCT CCGGATATAA CAACATTGGG 
GACTTTGAAC CCTACGTGGA CCCTTCATGG CCGGGGACCT CCTCACCGTG GATCTCAGTG 
GTGCCTTGGG GTATCAGGAG GCTGCTGAAC TTTGTGAAAG AGGAGTATGC AACAGGCAGT 
CTGCCATTAT ACATCACGGG GAATGGGATG CCGACGGCAC ACAATGTAGA GATGTACAAT 
GATCCAACCA GAGTGGACTA TTTAAAGGCC TACATTAACG AGGTCCTGAA AGCTGTCAAG 
ACTGATAATG TGCCAGTTGG AGCTTATATA GTCCGTTCCC TCCTGGATGG ATTTGAAGGC 
CCCCAAGGTT ACAGCCAGAG GTTTGGGTTA CACCACGTGG ATTTTGAGAA CGGCAACAGG 
CAGAGGACCC CCAAAGAATC AGCTTATTTC TTCCACAGAA TCATTGAAAA TAACGGCTTC 
CCCGTGACGA AGAAGACCAA CGTGCCACCG AATTCAAGAT GGCCGTTAGC AAAGAAGTTA 
CCTGCTCTGC CTTCTTCTGA GGTTCCTTCA AAGGCCCAAG TCGTATGGGA AACATTCTCA 
GGGCAAACTG ATTTTGAAAG GGATATGTAT TTCTATGGCA CCTTTCCAAG CGACTTCCAC 
TGGGGGGTTT CAACCTCGGC CTATCAGATA GAAGGTGGGT GGAACGCTGA TGGGAAGGGG 
CCGAGTACAT GGGACACCTA CACGCATATT CCTGGGAATA TTTATAATAA TGACAATGGG 
GACGTTGCCT GTGACAGCTA TAACCAAGCA GACGCAGACG TGTACATGCT CAGGGCCTTG 
GGAGTGACCA GCTATAGGTT CTCCTTGTCT TGGTCTCGGA TCTTCCCAAC TGGCACCGGC 
ACCCCCAATG CAAAAGGGGT GGATTATTAC AACGGACTTA TCAACAAACT TGTAGAAAAC 
CACATTGCCC CGATGGTGAC ACTTTACCAC TTTGACCTTC CCCAGGCTCT TCAGGATATA 
GGTGGTTGGG AGAGCGATGC CGTGCTTGAC GCATTTCACA GCTATGCCGA CTATTGCTTT 
AAGACCTTTG GAGACCGGGT CAAGTTCTGG ATGACGTTTA ACCAACCCCA CACTATTGTG 
ACAGCTGGGT ATGGCCTGGG GTTGATACCA CCTGGACTGA AAGACGACCC AGGATCTGCT 
CCTTATAGAG TAGCCCATAA TCTTTTGAAA GTCCATGCCA GGGTTTACCA TACCTACGAC 
CAACAGTATC GGGCTAGTCA AGGTGGCGTT ATCTCTATAA GTCTCAACAC AGAATGGGCA 
GAACCCAAAA ACCCCAAAGA CCCAAGAGAC GTGACAGCAG CTGACAGATA CTTACAGTTT 
TCACTCGGTT GGTTTGCCCA CCCCATATTC AAAAATGGTG ACTACCCAGA TGCCATGAAG 
TGGCAAGTGG CCAACAAAAG TGACTTACAG AACCTAAAAT CTTCCAGGCT GCCAAGCTTC 
ACGGAGGAGG AGAAGGCCTA CATCCAAGGA ACGGCTGATG TCTTCTGCAT TAACATCTAC 
ACTACGAAAA TAGTTCAATA TAAAACAATC TCCCTGAACC CTCCTTCTTT TGAAAGAGAC 
AGGGATACGG TGGAAGAGGT CAACCCACAG TGGCCAACAA TACCCTTCAT AAACAGCAGA 
GCAGTGGCAT GGGGGTTACG CCGGCTTCTT AACTGGGTGA AGGAGGAGTA TGGGAACGTT 
CCCACCTATG TCACGGAGAA TGGAGATGCA ACAGACGACG TCCCACCAGA TTATGATGAC 
ACCGCACGGA TCTTCTACTA CAAAACCTAC ATTGATGAGG CCTTGAAAGC TTACAGCTTG 
GATGGTGTAA ACCTAAAAGG CTATGCCCCG TGGTCTCTCA TGGACTCATT TGAATGGAAA 
TCAGGATACA GAGTGAGGTT CGGCCTGCAC TACGTGGACT TCAAAAACCC AAGCCGCCCA 
AGGACAGCAA AGCACTCTGC CATCTACTAC TCTCAAGTCA TACGCAAGAA CGGGATCCCA 
CAGGGCAGGG AGGATGAGTT CTTCTATGGG GAGTTCCCCA AGGACTTTGC ATGGAGTGTG 
GCCTCTGCAG CCTTCCAGAT TGAAGGGGGC TGGAGAGCAG ATGGGAAAGG TCTGAGCATC 
TGGGACCAGT TTACACACTC GCCATCCAGA ATCGAAGACG ACAGCAATGG GGACGTGGCC 
TGCAATAGCT ACAACATGAT GGAGAGGGAC GTGGAGATGC TGAGGAACCT CAAAGTCTCC 
CATTACCGAT TTTCAATATC GTGGCCAAGA GTCCTTCCAG ACGGGACCGT GAAGTCTTTC 
AATCAAGCCG GTCTGGATTA TTACATCCGC CTGATAGATG CTCTTCTGGC TGCCAATATA 
GAACCCCAGG TAACTTTGTA TCACTGGGAT CTTCCACAAG AGCTACAGAA CATGGGCGGT 
TGGGAAAATG AGACCATGGT GCAATGGTTT AAAGAGTACG CAGACTTGAT ATTCCAAAAA 
CTGGGAGACA AGGTGAAGAT GTGGATAACG TTCAATGAAC CGTACATTAT TGCCCTCCTT 
GGCTACGGCT ATGGCAACTT TGCCCCAGGT GTTAATGTAA GAATTGGGAC CGCCCCCTAC 
GTGGTGGGAC ACAACATTAT CAAGGCCCAC GCAGAGGCGT GGCATCTGTA CAATGATAAG 
TACAGGGCGG CACAAGGGGG CCTCATCTCC ATTACTGTCA ACTCCGATTG GGCAGAGCCA 
AGGAACCCCT ACAAGCAGGA GGATGTGGAG GCTGCTAGAA GATACTTGTC GTTTTTTTGC 
GGTTGGTTCG CTGATCCCAT TTTCCATGGA GACTACAACG AAGTCATGAA GACGAGGATC 
CTGGAGAGGA GTTTGGGTCA GGGGCTGACT ACATCCCGAC TCCCAGAATT CACGGAGAGC 
GAGAAGCAAA GAATTAAGGG AACTCATGAT TTCTTTGGGC TCAATCATTA CACCACGGTG 
CTGGCAGCGC CCCTGGACTT CCCAGAAGGA GACCCGACGT ATGACGCAGA CAGAGGTACC 
GCTGTGATGA GTGATCGGAC CTGGCTCGGT TCCGGCTCTA ACTGGCTGAG GGTCACCCCG 
TTTGGCCTAA GGAGGCTGCT CAACTGGATA AAGGAAACGT ACAACAACCC CCCCATTTAT 
GTGACAGAAA ACGGGATTTC GGAACGCGGG ACCAATCTGA AGGACGTATG GAGGGAACAT 
TATTATAAAT ATTATATCAA TGAGGCGCTG AAAGCTGTCA AATACGATGG AGCCGACCTC 
AGGGGTTACG CAGCCTGGAG CCTGATGGAT AACTTCGAGT GGGCAGCAGG ATACACGGAA 
AGGTTCGGAC TGTATTACGT CAACTTCACG GACCCCGGGG TCCCGCGTAT CCCTAAGGAT 
TCTGTAAAGT ACTACAGATC TATCATTCAG TGCAATGGTT TCCCAGACCC CAAAGATGGC 
ACCCCCCCTT GTCTTCAGCC TGAACCCGAA GGCACAGCTA AAACAACTGA CCAATCACAC 
GTCACTACTG CCCAACCAAT ACAAGGAGAT GAAGGAACGG TTTCCTTCCT GGGACTGGGA 
ATCTCCGCTG CGGACGCTCA GATCGCTCTG TACGTGGAAT TCGCGCTCAT GATCGCTGGG 
GTTCTAGGGA TTATACTATT TGGTGTTTTA TACAGCAAGT TGGCCAAAAA ATTTAAAAAG 
TCAGTAACAT CATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lct.2 ( XM_012970934.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826388.1 Xenopus tropicalis lactase, gene 2 (lct.2), mRNA.

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CloneID	OXa32043
Clone ID Related Accession (Same CDS sequence)	XM_012970934.1
Accession Version	XM_012970934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lactase-phlorizin hydrolase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGGT GCTCCCCCCC TGCCACACTG TGCATGATGG GTGCCTACAG GGTGCTACTC 
GCACTGCTGC TCTGCTCTCT GTACCAGGGA GCCCATAACC TGACACCCAA TGAGAAGCTT 
GTGGCCCTTG CTGGACCCAT AACAAGCAGT GTATTGGGGC CACTGAATGA GGGAAATGGA 
GACATTGGGT GCCATGATGC CCTTTTGCAG AAGCTTAGAG ACCGGCTGCC AACGCTGAGC 
CAATATGGCG CCACCCATTA TAAGCTGAAC CTACAGTGGG CTGCCCTGTT CCACAAGGGG 
TATGATCAGA CAGGGCAAGT CCAGTGCTAC CGGAACCTGC TCCAACTGCT GGTCAGTGAG 
AACATGAAGC CGGTGGTCAT TCTGCAAGGA GAGCGCAGCA GTGGCCTCTT GCTTTCCGCT 
GCCAGTAGCC AAGCGGCCAG TGACTTGGCC AATGAGTATG TGGCCCATGC TGACTTTGCC 
TTCAATGTGT TCGGTGATTT GGTTCAGACC TGGATCACCT TTGATGTTCC CACAGGACAG 
AGCGGGGAGG CATCTCCTGG GGGCCTGAAG GCCCTGCTCC GTGCCCACCA GAGAACCCAT 
GACTTGTACA GGGGCAAATA CTCTTCCAAA GGTGGGAGAC TGTCTGTTGC CATGGAACCG 
GCCTTACTGG AGGCATCTCT GTCCCTTCAG CCACGTCACC GGGAGTTTGT TGATTTTGTT 
GCTCTGAAGA TAAACTACAG TTGCAAGAAT GGAATCAGCT TGGAGAAACC TCATTGGGAA 
CAGAGTATCT GGAAGGGGCT GCAGGTTCTG GTTTTCCAGT TGAAGCTCGG GGGCTGCGCT 
TCTCTATCAG ATGAAAGTAG ATATCTCCCG ACCTACCAAC TCGCTGCAGG TCTGAGCAGC 
GCCGGGGCAG TGTTTCTGGG GTACGACATC AGCGCCTTCA TAGACTATTC GCCCATAACT 
AAGGAAAGGG TGGAACAAGG TGCAGAGAAG TCCCTCTTTG CCCCTCTAAT TGCCCCTCGT 
TCCTCCTACA CTACAGTCTG GGACCGATTT GCTAACCAGG CCCCGGCCGA GAGAGACTCC 
TTCCTGCAGG ACGTGTTTCC CAGTGGGTTC TGGTGGGGCT CATCCACTGC TGCTTTCAGA 
GTGGAAGGGG GTTGGGCAGA AGGGGGGAAA GGAGAAACCA TCTGGGACAG GTTTGGCCAT 
CAAAACCTGG CAGCACAGAA TGCCACCGCC AACGTGGCCT CCGACAGCTA CCGGAAAACT 
GACTACGACG TCTATCTTCT CAAGGGCCTG CAGTCCAACG TGTACAAGTT CTCCATCGCC 
TGGTCCAGGA TCTTCCCAAA TGGGCTCAAA TCCGAGGGGA GTAGAAAGGG GGTGGAATAC 
TACAATACAT TAATTGACAG CTTAAGAGCC GCAGGGGTGG AACCAATGGT TACTTTATTC 
CACTGGGATC TGCCCCAACC TCTGCAGGAC CTTGGGGGCT GGACAAACGA GAACACGGTA 
GATGCCTTTG TAGACTACGC CGATTTCTGT TTCAGCCATT TTGGAGACCG TGTCAAACTC 
TGGATCACGT TTCATGAGCC GTGGGTCGTC AGCTACGCTG GTTATGGAAC TGGGGAACAT 
GCCCCAGGGA TTAAGGACCC TGGAAATGCT TCTTACAAGG TGGCCCACAA CATCATCAAA 
GCTCACGCAA AAGCCTGGCA TTTGTACGAC GGCCAATACC GTGCCCATCA ACAGGGCAAA 
GTGGGAATCT CTCTGAACTC AGACTGGGCG GAGCCAGCAT CCCCGGCCAA TCCTGCTGAT 
GTTGAGGCGG CTGAGCGATA CCTTCAGTTC ATGCTGGGCT GGTTTGCACA CCCTATACTG 
GTCGATGGGG ACTACCCAGC CGTTCTCAAA ACCCAAATAC AGAAGAAGAA CCAGCAGTGC 
CCTGGAACAG TCTCCCAGTT GCCCACCTTC ACCGAGGTGG AAAAATCCAG CATCCATGGA 
ACTGCAGACT TCCTGGGAAT TTCCCATTAT ACCTCCAGGC TGGTCAATGC ATCTGTTAGT 
GCAGCCTGTG TCTCCGGATA TAACAACATT GGGGACTTTG AACCCTACGT GGACCCTTCA 
TGGCCGGGGA CCTCCTCACC GTGGATCTCA GTGGTGCCTT GGGGTATCAG GAGGCTGCTG 
AACTTTGTGA AAGAGGAGTA TGCAACAGGC AGTCTGCCAT TATACATCAC GGGGAATGGG 
ATGCCGACGG CACACAATGT AGAGATGTAC AATGATCCAA CCAGAGTGGA CTATTTAAAG 
GCCTACATTA ACGAGGTCCT GAAAGCTGTC AAGACTGATA ATGTGCCAGT TGGAGCTTAT 
ATAGTCCGTT CCCTCCTGGA TGGATTTGAA GGCCCCCAAG GTTACAGCCA GAGGTTTGGG 
TTACACCACG TGGATTTTGA GAACGGCAAC AGGCAGAGGA CCCCCAAAGA ATCAGCTTAT 
TTCTTCCACA GAATCATTGA AAATAACGGC TTCCCCGTGA CGAAGAAGAC CAACGTGCCA 
CCGAATTCAA GATGGCCGTT AGCAAAGAAG TTACCTGCTC TGCCTTCTTC TGAGGTTCCT 
TCAAAGGCCC AAGTCGTATG GGAAACATTC TCAGGGCAAA CTGATTTTGA AAGGGATATG 
TATTTCTATG GCACCTTTCC AAGCGACTTC CACTGGGGGG TTTCAACCTC GGCCTATCAG 
ATAGAAGGTG GGTGGAACGC TGATGGGAAG GGGCCGAGTA CATGGGACAC CTACACGCAT 
ATTCCTGGGA ATATTTATAA TAATGACAAT GGGGACGTTG CCTGTGACAG CTATAACCAA 
GCAGACGCAG ACGTGTACAT GCTCAGGGCC TTGGGAGTGA CCAGCTATAG GTTCTCCTTG 
TCTTGGTCTC GGATCTTCCC AACTGGCACC GGCACCCCCA ATGCAAAAGG GGTGGATTAT 
TACAACGGAC TTATCAACAA ACTTGTAGAA AACCACATTG CCCCGATGGT GACACTTTAC 
CACTTTGACC TTCCCCAGGC TCTTCAGGAT ATAGGTGGTT GGGAGAGCGA TGCCGTGCTT 
GACGCATTTC ACAGCTATGC CGACTATTGC TTTAAGACCT TTGGAGACCG GGTCAAGTTC 
TGGATGACGT TTAACCAACC CCACACTATT GTGACAGCTG GGTATGGCCT GGGGTTGATA 
CCACCTGGAC TGAAAGACGA CCCAGGATCT GCTCCTTATA GAGTAGCCCA TAATCTTTTG 
AAAGTCCATG CCAGGGTTTA CCATACCTAC GACCAACAGT ATCGGGCTAG TCAAGGTGGC 
GTTATCTCTA TAAGTCTCAA CACAGAATGG GCAGAACCCA AAAACCCCAA AGACCCAAGA 
GACGTGACAG CAGCTGACAG ATACTTACAG TTTTCACTCG GTTGGTTTGC CCACCCCATA 
TTCAAAAATG GTGACTACCC AGATGCCATG AAGTGGCAAG TGGCCAACAA AAGTGACTTA 
CAGAACCTAA AATCTTCCAG GCTGCCAAGC TTCACGGAGG AGGAGAAGGC CTACATCCAA 
GGAACGGCTG ATGTCTTCTG CATTAACATC TACACTACGA AAATAGTTCA ATATAAAACA 
ATCTCCCTGA ACCCTCCTTC TTTTGAAAGA GACAGGGATA CGGTGGAAGA GGTCAACCCA 
CAGTGGCCAA CAATACCCTT CATAAACAGC AGAGCAGTGG CATGGGGGTT ACGCCGGCTT 
CTTAACTGGG TGAAGGAGGA GTATGGGAAC GTTCCCACCT ATGTCACGGA GAATGGAGAT 
GCAACAGACG ACGTCCCACC AGATTATGAT GACACCGCAC GGATCTTCTA CTACAAAACC 
TACATTGATG AGGCCTTGAA AGCTTACAGC TTGGATGGTG TAAACCTAAA AGGCTATGCC 
CCGTGGTCTC TCATGGACTC ATTTGAATGG AAATCAGGAT ACAGAGTGAG GTTCGGCCTG 
CACTACGTGG ACTTCAAAAA CCCAAGCCGC CCAAGGACAG CAAAGCACTC TGCCATCTAC 
TACTCTCAAG TCATACGCAA GAACGGGATC CCACAGGGCA GGGAGGATGA GTTCTTCTAT 
GGGGAGTTCC CCAAGGACTT TGCATGGAGT GTGGCCTCTG CAGCCTTCCA GATTGAAGGG 
GGCTGGAGAG CAGATGGGAA AGGTCTGAGC ATCTGGGACC AGTTTACACA CTCGCCATCC 
AGAATCGAAG ACGACAGCAA TGGGGACGTG GCCTGCAATA GCTACAACAT GATGGAGAGG 
GACGTGGAGA TGCTGAGGAA CCTCAAAGTC TCCCATTACC GATTTTCAAT ATCGTGGCCA 
AGAGTCCTTC CAGACGGGAC CGTGAAGTCT TTCAATCAAG CCGGTCTGGA TTATTACATC 
CGCCTGATAG ATGCTCTTCT GGCTGCCAAT ATAGAACCCC AGGTAACTTT GTATCACTGG 
GATCTTCCAC AAGAGCTACA GAACATGGGC GGTTGGGAAA ATGAGACCAT GGTGCAATGG 
TTTAAAGAGT ACGCAGACTT GATATTCCAA AAACTGGGAG ACAAGGTGAA GATGTGGATA 
ACGTTCAATG AACCGTACAT TATTGCCCTC CTTGGCTACG GCTATGGCAA CTTTGCCCCA 
GGTGTTAATG AAAGAATTGG GACCGCCCCC TACGTGGTGG GACACAACAT TATCAAGGCC 
CACGCAGAGG CGTGGCATCT GTACAATGAT AAGTACAGGG CGGCACAAGG GGGCCTCATC 
TCCATTACTG TCAACTCCGA TTGGGCAGAG CCAAGGAACC CCTACAAGCA GGAGGATGTG 
GAGGCTGCTA GAAGATACTT GTCGTTTTTT TGCGGTTGGT TCGCTGATCC CATTTTCCAT 
GGAGACTACA ACCAAGTCAT GAAGTCGCGG ATCATGGAGA GGAGTTTGGG TCAGGGGCTG 
ACTACATCCC GACTCCCAGA ATTCACGGAG AGCGAGAAGC AAAGAATTAA GGGAACTCAT 
GATTTCTTTG GGCTCAATCA TTACACCACG GTGCTGGCAG CGCCCCTGGA CTTCCCAGAA 
GGAGACCCGA CGTATGACGC AGACAGAGGT ACCGCTGTGA TGAGTGATCG GACCTGGCTC 
GGTTCCGGCT CTAACTGGCT GAGGGTCACC CCGTTTGGCC TAAGGAGGCT GCTCAACTGG 
ATAAAGGAAA CGTACAACAA CCCCCCCATT TATGTGACAG AAAACGGGAT TTCGGAACGC 
GGGACCAATC TGAAGGACGT ATGGAGGGAA CATTATTATA AATATTATAT CAATGAGGCG 
CTGAAAGCTG TCAAATACGA TGGAGCCGAC CTCAGGGGTT ACGCAGCCTG GAGCCTGATG 
GATAACTTCG AGTGGGCAGC AGGATACACG GAAAGGTTCG GACTGTATTA CGTCAACTTC 
ACGGACCCCG GGGTCCCGCG TATCCCTAAG GATTCTGTAA AGTACTACAG ATCTATCATT 
CAGTGCAATG GTTTCCCAGA CCCCAAAGAT GGCACCCCCC CTTGTCTTCA GCCTGAACCC 
GAAGGCACAG CTAAAACAAC TGACCAATCA CACGTCACTA CTGCCCAACC AATACAAGGA 
GATGAAGGAA CGGTTTCCTT CCTGGGACTG GGAATCTCCG CTGCGGACGC TCAGATCGCT 
CTGTACGTGG AATTCGCGCT CATGATCGCT GGGGTTCTAG GGATTATCCT ATTTGGTGTT 
TTATACAGCA AGTTGGCCAA AAAATTTAAA AAGTCAGTAA CATCATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826388.1
CDS	12..5762
Translation	MPGCSPPATLCMMGAYRVLLALLLCSLYQGAHNLTPNEKLVALAGPITSSVLGPLNEGNGDIGCHDALLQKLRDRLPTLSQYGATHYKLNLQWAALFHKGYDQTGQVQCYRNLLQLLVSENMKPVVILQGERSSGLLLSAASSQAASDLANEYVAHADFAFNVFGDLVQTWITFDVPTGQSGEASPGGLKALLRAHQRTHDLYRGKYSSKGGRLSVAMEPALLEASLSLQPRHREFVDFVALKINYSCKNGISLEKPHWEQSIWKGLQVLVFQLKLGGCASLSDESRYLPTYQLAAGLSSAGAVFLGYDISAFIDYSPITKERVEQGAEKSLFAPLIAPRSSYTTVWDRFANQAPAERDSFLQDVFPSGFWWGSSTAAFRVEGGWAEGGKGETIWDRFGHQNLAAQNATANVASDSYRKTDYDVYLLKGLQSNVYKFSIAWSRIFPNGLKSEGSRKGVEYYNTLIDSLRAAGVEPMVTLFHWDLPQPLQDLGGWTNENTVDAFVDYADFCFSHFGDRVKLWITFHEPWVVSYAGYGTGEHAPGIKDPGNASYKVAHNIIKAHAKAWHLYDGQYRAHQQGKVGISLNSDWAEPASPANPADVEAAERYLQFMLGWFAHPILVDGDYPAVLKTQIQKKNQQCPGTVSQLPTFTEVEKSSIHGTADFLGISHYTSRLVNASVSAACVSGYNNIGDFEPYVDPSWPGTSSPWISVVPWGIRRLLNFVKEEYATGSLPLYITGNGMPTAHNVEMYNDPTRVDYLKAYINEVLKAVKTDNVPVGAYIVRSLLDGFEGPQGYSQRFGLHHVDFENGNRQRTPKESAYFFHRIIENNGFPVTKKTNVPPNSRWPLAKKLPALPSSEVPSKAQVVWETFSGQTDFERDMYFYGTFPSDFHWGVSTSAYQIEGGWNADGKGPSTWDTYTHIPGNIYNNDNGDVACDSYNQADADVYMLRALGVTSYRFSLSWSRIFPTGTGTPNAKGVDYYNGLINKLVENHIAPMVTLYHFDLPQALQDIGGWESDAVLDAFHSYADYCFKTFGDRVKFWMTFNQPHTIVTAGYGLGLIPPGLKDDPGSAPYRVAHNLLKVHARVYHTYDQQYRASQGGVISISLNTEWAEPKNPKDPRDVTAADRYLQFSLGWFAHPIFKNGDYPDAMKWQVANKSDLQNLKSSRLPSFTEEEKAYIQGTADVFCINIYTTKIVQYKTISLNPPSFERDRDTVEEVNPQWPTIPFINSRAVAWGLRRLLNWVKEEYGNVPTYVTENGDATDDVPPDYDDTARIFYYKTYIDEALKAYSLDGVNLKGYAPWSLMDSFEWKSGYRVRFGLHYVDFKNPSRPRTAKHSAIYYSQVIRKNGIPQGREDEFFYGEFPKDFAWSVASAAFQIEGGWRADGKGLSIWDQFTHSPSRIEDDSNGDVACNSYNMMERDVEMLRNLKVSHYRFSISWPRVLPDGTVKSFNQAGLDYYIRLIDALLAANIEPQVTLYHWDLPQELQNMGGWENETMVQWFKEYADLIFQKLGDKVKMWITFNEPYIIALLGYGYGNFAPGVNERIGTAPYVVGHNIIKAHAEAWHLYNDKYRAAQGGLISITVNSDWAEPRNPYKQEDVEAARRYLSFFCGWFADPIFHGDYNQVMKSRIMERSLGQGLTTSRLPEFTESEKQRIKGTHDFFGLNHYTTVLAAPLDFPEGDPTYDADRGTAVMSDRTWLGSGSNWLRVTPFGLRRLLNWIKETYNNPPIYVTENGISERGTNLKDVWREHYYKYYINEALKAVKYDGADLRGYAAWSLMDNFEWAAGYTERFGLYYVNFTDPGVPRIPKDSVKYYRSIIQCNGFPDPKDGTPPCLQPEPEGTAKTTDQSHVTTAQPIQGDEGTVSFLGLGISAADAQIALYVEFALMIAGVLGIILFGVLYSKLAKKFKKSVTSF

Target ORF information:

RefSeq Version	XM_012970934.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lactase, gene 2 (lct.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970934.1

ORF Insert Sequence:

ATGCCTGGGT GCTCCCCCCC TGCCACACTG TGCATGATGG GTGCCTACAG GGTGCTACTC 
GCACTGCTGC TCTGCTCTCT GTACCAGGGA GCCCATAACC TGACACCCAA TGAGAAGCTT 
GTGGCCCTTG CTGGACCCAT AACAAGCAGT GTATTGGGGC CACTGAATGA GGGAAATGGA 
GACATTGGGT GCCATGATGC CCTTTTGCAG AAGCTTAGAG ACCGGCTGCC AACGCTGAGC 
CAATATGGCG CCACCCATTA TAAGCTGAAC CTACAGTGGG CTGCCCTGTT CCACAAGGGG 
TATGATCAGA CAGGGCAAGT CCAGTGCTAC CGGAACCTGC TCCAACTGCT GGTCAGTGAG 
AACATGAAGC CGGTGGTCAT TCTGCAAGGA GAGCGCAGCA GTGGCCTCTT GCTTTCCGCT 
GCCAGTAGCC AAGCGGCCAG TGACTTGGCC AATGAGTATG TGGCCCATGC TGACTTTGCC 
TTCAATGTGT TCGGTGATTT GGTTCAGACC TGGATCACCT TTGATGTTCC CACAGGACAG 
AGCGGGGAGG CATCTCCTGG GGGCCTGAAG GCCCTGCTCC GTGCCCACCA GAGAACCCAT 
GACTTGTACA GGGGCAAATA CTCTTCCAAA GGTGGGAGAC TGTCTGTTGC CATGGAACCG 
GCCTTACTGG AGGCATCTCT GTCCCTTCAG CCACGTCACC GGGAGTTTGT TGATTTTGTT 
GCTCTGAAGA TAAACTACAG TTGCAAGAAT GGAATCAGCT TGGAGAAACC TCATTGGGAA 
CAGAGTATCT GGAAGGGGCT GCAGGTTCTG GTTTTCCAGT TGAAGCTCGG GGGCTGCGCT 
TCTCTATCAG ATGAAAGTAG ATATCTCCCG ACCTACCAAC TCGCTGCAGG TCTGAGCAGC 
GCCGGGGCAG TGTTTCTGGG GTACGACATC AGCGCCTTCA TAGACTATTC GCCCATAACT 
AAGGAAAGGG TGGAACAAGG TGCAGAGAAG TCCCTCTTTG CCCCTCTAAT TGCCCCTCGT 
TCCTCCTACA CTACAGTCTG GGACCGATTT GCTAACCAGG CCCCGGCCGA GAGAGACTCC 
TTCCTGCAGG ACGTGTTTCC CAGTGGGTTC TGGTGGGGCT CATCCACTGC TGCTTTCAGA 
GTGGAAGGGG GTTGGGCAGA AGGGGGGAAA GGAGAAACCA TCTGGGACAG GTTTGGCCAT 
CAAAACCTGG CAGCACAGAA TGCCACCGCC AACGTGGCCT CCGACAGCTA CCGGAAAACT 
GACTACGACG TCTATCTTCT CAAGGGCCTG CAGTCCAACG TGTACAAGTT CTCCATCGCC 
TGGTCCAGGA TCTTCCCAAA TGGGCTCAAA TCCGAGGGGA GTAGAAAGGG GGTGGAATAC 
TACAATACAT TAATTGACAG CTTAAGAGCC GCAGGGGTGG AACCAATGGT TACTTTATTC 
CACTGGGATC TGCCCCAACC TCTGCAGGAC CTTGGGGGCT GGACAAACGA GAACACGGTA 
GATGCCTTTG TAGACTACGC CGATTTCTGT TTCAGCCATT TTGGAGACCG TGTCAAACTC 
TGGATCACGT TTCATGAGCC GTGGGTCGTC AGCTACGCTG GTTATGGAAC TGGGGAACAT 
GCCCCAGGGA TTAAGGACCC TGGAAATGCT TCTTACAAGG TGGCCCACAA CATCATCAAA 
GCTCACGCAA AAGCCTGGCA TTTGTACGAC GGCCAATACC GTGCCCATCA ACAGGGCAAA 
GTGGGAATCT CTCTGAACTC AGACTGGGCG GAGCCAGCAT CCCCGGCCAA TCCTGCTGAT 
GTTGAGGCGG CTGAGCGATA CCTTCAGTTC ATGCTGGGCT GGTTTGCACA CCCTATACTG 
GTCGATGGGG ACTACCCAGC CGTTCTCAAA ACCCAAATAC AGAAGAAGAA CCAGCAGTGC 
CCTGGAACAG TCTCCCAGTT GCCCACCTTC ACCGAGGTGG AAAAATCCAG CATCCATGGA 
ACTGCAGACT TCCTGGGAAT TTCCCATTAT ACCTCCAGGC TGGTCAATGC ATCTGTTAGT 
GCAGCCTGTG TCTCCGGATA TAACAACATT GGGGACTTTG AACCCTACGT GGACCCTTCA 
TGGCCGGGGA CCTCCTCACC GTGGATCTCA GTGGTGCCTT GGGGTATCAG GAGGCTGCTG 
AACTTTGTGA AAGAGGAGTA TGCAACAGGC AGTCTGCCAT TATACATCAC GGGGAATGGG 
ATGCCGACGG CACACAATGT AGAGATGTAC AATGATCCAA CCAGAGTGGA CTATTTAAAG 
GCCTACATTA ACGAGGTCCT GAAAGCTGTC AAGACTGATA ATGTGCCAGT TGGAGCTTAT 
ATAGTCCGTT CCCTCCTGGA TGGATTTGAA GGCCCCCAAG GTTACAGCCA GAGGTTTGGG 
TTACACCACG TGGATTTTGA GAACGGCAAC AGGCAGAGGA CCCCCAAAGA ATCAGCTTAT 
TTCTTCCACA GAATCATTGA AAATAACGGC TTCCCCGTGA CGAAGAAGAC CAACGTGCCA 
CCGAATTCAA GATGGCCGTT AGCAAAGAAG TTACCTGCTC TGCCTTCTTC TGAGGTTCCT 
TCAAAGGCCC AAGTCGTATG GGAAACATTC TCAGGGCAAA CTGATTTTGA AAGGGATATG 
TATTTCTATG GCACCTTTCC AAGCGACTTC CACTGGGGGG TTTCAACCTC GGCCTATCAG 
ATAGAAGGTG GGTGGAACGC TGATGGGAAG GGGCCGAGTA CATGGGACAC CTACACGCAT 
ATTCCTGGGA ATATTTATAA TAATGACAAT GGGGACGTTG CCTGTGACAG CTATAACCAA 
GCAGACGCAG ACGTGTACAT GCTCAGGGCC TTGGGAGTGA CCAGCTATAG GTTCTCCTTG 
TCTTGGTCTC GGATCTTCCC AACTGGCACC GGCACCCCCA ATGCAAAAGG GGTGGATTAT 
TACAACGGAC TTATCAACAA ACTTGTAGAA AACCACATTG CCCCGATGGT GACACTTTAC 
CACTTTGACC TTCCCCAGGC TCTTCAGGAT ATAGGTGGTT GGGAGAGCGA TGCCGTGCTT 
GACGCATTTC ACAGCTATGC CGACTATTGC TTTAAGACCT TTGGAGACCG GGTCAAGTTC 
TGGATGACGT TTAACCAACC CCACACTATT GTGACAGCTG GGTATGGCCT GGGGTTGATA 
CCACCTGGAC TGAAAGACGA CCCAGGATCT GCTCCTTATA GAGTAGCCCA TAATCTTTTG 
AAAGTCCATG CCAGGGTTTA CCATACCTAC GACCAACAGT ATCGGGCTAG TCAAGGTGGC 
GTTATCTCTA TAAGTCTCAA CACAGAATGG GCAGAACCCA AAAACCCCAA AGACCCAAGA 
GACGTGACAG CAGCTGACAG ATACTTACAG TTTTCACTCG GTTGGTTTGC CCACCCCATA 
TTCAAAAATG GTGACTACCC AGATGCCATG AAGTGGCAAG TGGCCAACAA AAGTGACTTA 
CAGAACCTAA AATCTTCCAG GCTGCCAAGC TTCACGGAGG AGGAGAAGGC CTACATCCAA 
GGAACGGCTG ATGTCTTCTG CATTAACATC TACACTACGA AAATAGTTCA ATATAAAACA 
ATCTCCCTGA ACCCTCCTTC TTTTGAAAGA GACAGGGATA CGGTGGAAGA GGTCAACCCA 
CAGTGGCCAA CAATACCCTT CATAAACAGC AGAGCAGTGG CATGGGGGTT ACGCCGGCTT 
CTTAACTGGG TGAAGGAGGA GTATGGGAAC GTTCCCACCT ATGTCACGGA GAATGGAGAT 
GCAACAGACG ACGTCCCACC AGATTATGAT GACACCGCAC GGATCTTCTA CTACAAAACC 
TACATTGATG AGGCCTTGAA AGCTTACAGC TTGGATGGTG TAAACCTAAA AGGCTATGCC 
CCGTGGTCTC TCATGGACTC ATTTGAATGG AAATCAGGAT ACAGAGTGAG GTTCGGCCTG 
CACTACGTGG ACTTCAAAAA CCCAAGCCGC CCAAGGACAG CAAAGCACTC TGCCATCTAC 
TACTCTCAAG TCATACGCAA GAACGGGATC CCACAGGGCA GGGAGGATGA GTTCTTCTAT 
GGGGAGTTCC CCAAGGACTT TGCATGGAGT GTGGCCTCTG CAGCCTTCCA GATTGAAGGG 
GGCTGGAGAG CAGATGGGAA AGGTCTGAGC ATCTGGGACC AGTTTACACA CTCGCCATCC 
AGAATCGAAG ACGACAGCAA TGGGGACGTG GCCTGCAATA GCTACAACAT GATGGAGAGG 
GACGTGGAGA TGCTGAGGAA CCTCAAAGTC TCCCATTACC GATTTTCAAT ATCGTGGCCA 
AGAGTCCTTC CAGACGGGAC CGTGAAGTCT TTCAATCAAG CCGGTCTGGA TTATTACATC 
CGCCTGATAG ATGCTCTTCT GGCTGCCAAT ATAGAACCCC AGGTAACTTT GTATCACTGG 
GATCTTCCAC AAGAGCTACA GAACATGGGC GGTTGGGAAA ATGAGACCAT GGTGCAATGG 
TTTAAAGAGT ACGCAGACTT GATATTCCAA AAACTGGGAG ACAAGGTGAA GATGTGGATA 
ACGTTCAATG AACCGTACAT TATTGCCCTC CTTGGCTACG GCTATGGCAA CTTTGCCCCA 
GGTGTTAATG AAAGAATTGG GACCGCCCCC TACGTGGTGG GACACAACAT TATCAAGGCC 
CACGCAGAGG CGTGGCATCT GTACAATGAT AAGTACAGGG CGGCACAAGG GGGCCTCATC 
TCCATTACTG TCAACTCCGA TTGGGCAGAG CCAAGGAACC CCTACAAGCA GGAGGATGTG 
GAGGCTGCTA GAAGATACTT GTCGTTTTTT TGCGGTTGGT TCGCTGATCC CATTTTCCAT 
GGAGACTACA ACCAAGTCAT GAAGTCGCGG ATCATGGAGA GGAGTTTGGG TCAGGGGCTG 
ACTACATCCC GACTCCCAGA ATTCACGGAG AGCGAGAAGC AAAGAATTAA GGGAACTCAT 
GATTTCTTTG GGCTCAATCA TTACACCACG GTGCTGGCAG CGCCCCTGGA CTTCCCAGAA 
GGAGACCCGA CGTATGACGC AGACAGAGGT ACCGCTGTGA TGAGTGATCG GACCTGGCTC 
GGTTCCGGCT CTAACTGGCT GAGGGTCACC CCGTTTGGCC TAAGGAGGCT GCTCAACTGG 
ATAAAGGAAA CGTACAACAA CCCCCCCATT TATGTGACAG AAAACGGGAT TTCGGAACGC 
GGGACCAATC TGAAGGACGT ATGGAGGGAA CATTATTATA AATATTATAT CAATGAGGCG 
CTGAAAGCTG TCAAATACGA TGGAGCCGAC CTCAGGGGTT ACGCAGCCTG GAGCCTGATG 
GATAACTTCG AGTGGGCAGC AGGATACACG GAAAGGTTCG GACTGTATTA CGTCAACTTC 
ACGGACCCCG GGGTCCCGCG TATCCCTAAG GATTCTGTAA AGTACTACAG ATCTATCATT 
CAGTGCAATG GTTTCCCAGA CCCCAAAGAT GGCACCCCCC CTTGTCTTCA GCCTGAACCC 
GAAGGCACAG CTAAAACAAC TGACCAATCA CACGTCACTA CTGCCCAACC AATACAAGGA 
GATGAAGGAA CGGTTTCCTT CCTGGGACTG GGAATCTCCG CTGCGGACGC TCAGATCGCT 
CTGTACGTGG AATTCGCGCT CATGATCGCT GGGGTTCTAG GGATTATCCT ATTTGGTGTT 
TTATACAGCA AGTTGGCCAA AAAATTTAAA AAGTCAGTAA CATCATTTTG A