tle1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tle1
Entrez Gene ID	100101799
Full Name	transducin like enhancer of split 1
Synonyms	esg,esg1,grg1,xgrg1
General protein information	Preferred Names transducin like enhancer of split 1 Names transducin-like enhancer protein 1 transducin-like enhancer of split 1 (E(sp1) homolog)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012961206.2	XP_012816660.1	transducin-like enhancer protein 1 isoform X1
NM_001170503.1	NP_001163974.1	transducin-like enhancer protein 1
XM_012961241.2	XP_012816695.1	transducin-like enhancer protein 1 isoform X2
XM_012961206.3	XP_012816660.1	transducin-like enhancer protein 1 isoform X1
XM_012961241.3	XP_012816695.1	transducin-like enhancer protein 1 isoform X2
XM_018092648.1	XP_017948137.1	transducin-like enhancer protein 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLE1	NP_005068.2
Gallus gallus (chicken)	TLE1	XP_004949238.1
Rattus norvegicus (Norway rat)	Tle1	NP_001166904.1
Canis lupus familiaris (dog)	TLE1	XP_533516.3
Mus musculus (house mouse)	Tle1	NP_035729.3
Pan troglodytes (chimpanzee)	LOC464546	XP_003312170.1
	TLE1	XP_001104021.1
Xenopus tropicalis (tropical clawed frog)	tle1	NP_001163974.1

Gene Ontology
Bibliography

Related articles in PubMed

Occupancy of tissue-specific cis-regulatory modules by Otx2 and TLE/Groucho for embryonic head specification.
Yasuoka Y, Suzuki Y, Takahashi S, Someya H, Sudou N, Haramoto Y, Cho KW, Asashima M, Sugano S, Taira M
Nature communications54322(2014 Jul)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Occupancy of the corepressor, TLE1, is a better indicator of tissue-specific cis-regulatory modules than the coactivator p300, during early embryonic stages.
Title: Occupancy of tissue-specific cis-regulatory modules by Otx2 and TLE/Groucho for embryonic head specification.

The following tle1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tle1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa23295	NM_001170503.1 Latest version!	Xenopus tropicalis transducin like enhancer of split 1 (tle1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa30284	XM_012961206.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa30285	XM_012961241.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa41633	XM_018092648.1 Latest version!	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OXa30284	XM_012961206.2	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa30285	XM_012961241.2	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tle1 ( NM_001170503.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001163974.1 Xenopus tropicalis transducin like enhancer of split 1 (tle1), mRNA.

Price & Availability↑

CloneID	OXa23295
Clone ID Related Accession (Same CDS sequence)	NM_001170503.1
Accession Version	NM_001170503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin-like enhancer protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU014558.1. ##Evidence-Data-START## Transcript exon combination :: GU014558.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGTTTCCAC AAAGCAGGCA CCCGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGGTACGTGG CCTTCCAGGT CTACCTCCTA CACAACAGCA GTTACAGGCC 
CAGCATCTAT CCCACAGTCA TGGACCGCCA GTGCCTCTGA CACCACATCC ATCAGGTCTT 
CAACCCCAAG GAATTCCACC TCTGGGAAGT AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT 
CTCAGTGGCC AGTCTCACCT AGCCATGAAA GATGACAAGA AGCACCATGA TTCAGACCAC 
CATAGAGACA GAGAGCCAGG AACAAGTAAT TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT 
ACTGATAAAA GAAGGAATGG TCCTGATTAT TCTAATGACA TAAAAAAAAG AAAGATTGAG 
GACAAGGATT CCAGTCACTA TGACAGTGAT GGAGACAAAA GTGATGATAA CTTGGTTGTG 
GATGTATCCA ATGAGGATCC TACATCTCCT AGAGTGAGTC CTGCTCACTC ACCTAGAGAA 
AACGGAATTG ATAAAAGTAG GTTACTTAAG AAAGATGCTT CAAGCAGTCC CGCATCCACT 
GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC 
AGCACGCCTG TTCTGAAATC AAGCACACCA ACTCCTCGAA CTGATGTGCC GACACCCGGC 
ACAAGTGTGA CGTCAGGGTT GCGACCAGGT CTTGGCAAAC CACCATCTAT GGATCCCCTC 
ATTAACCAAG CTGCTGCTGC TGGCTTGAGA ACACCTTTGG CAGTTCCTGG TCCCTATGCC 
GGTCCATTTG GAATGGTACC ACATGCAGGC ATGAATGGAG AACTGACGAG TCCAGGAGCT 
GCCTATGCTA GCTTGCACAA TATGTCCCCC CATATGAGTG CAGCTGCAGC AGCTGCTGTT 
GTAGCTTATG GACGATCGCC AATGTCTGGA TTTGACTCTC CACCACACAT GCGAGTGCCA 
GGAATGCCTC CAAATTTAGC TGGAATCCCT GGTGGCAAAC CGGCCTATTC GTTTCATGTT 
ACAGCAGATG GTCAAATGCA GCCAGTCCCT TTCCCTCCTG ATGCACTTAT AGGACCAGGC 
ATTCCAAGAC ATGCACGGCA GATAAACACA CTAAATCACG GGGAGGTAGT GTGCGCAGTT 
ACCATCAGTA ACCCCACGAG ACATGTGTAC ACTGGTGGGA AGGGATGTGT TAAAGTCTGG 
GATATCAGCC AACCTGGCAA CAAAAGCCCT GTCTCTCAGC TGGATTGCCT GAACAGAGAT 
AACTACATCA GATCATGTAA ATTGCTGCCT GATGGATGCA CTCTTATTGT GGGAGGGGAA 
GCCAGCACAT TATCCATTTG GGACCTGGCA GCTCCTACTC CACGTATAAA AGCAGAACTG 
ACATCATCTG CTCCAGCCTG CTATGCTCTG GCCATCAGCC CTGATTCCAA GGTCTGCTTC 
TCCTGCTGTA GTGATGGCAA CATTGCAGTC TGGGATTTGC ACAACCAGAC TCTAGTAAGG 
CAGTTCCAGG GCCACACAGA TGGAGCCAGC TGTATTGACA TTTCTAATGA TGGTACCAAG 
CTCTGGACCG GGGGTTTGGA CAACACGGTA AGGTCCTGGG ACCTGCGTGA AGGACGGCAG 
CTCCAGCAGC ATGACTTTAC ATCACAGATC TTCTCTCTTG GGTATTGCCC AACTGGTGAA 
TGGCTTGCTG TTGGAATGGA GAGTAGTAAT GTGGAAGTAC TGCATGTAAA CAAGCCAGAC 
AAGTATCAGC TGCACCTGCA TGAAAGCTGT GTACTCTCAC TAAAATTTGC CTATTGCGGC 
AAATGGTTTG TAAGTACTGG AAAGGATAAT CTTCTTAATG CTTGGCGAAC CCCATATGGA 
GCCAGTATAT TCCAGTCCAA AGAATCCTCA TCTGTACTGA GCTGCGATAT CTCTATCGAT 
GACAAATACA TTGTCACTGG GTCAGGGGAC AAGAAGGCCA CAGTCTATGA AGTCATCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163974.1
CDS	836..3178
Translation	MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGVRGLPGLPPTQQQLQAQHLSHSHGPPVPLTPHPSGLQPQGIPPLGSSAGLLALSSALSGQSHLAMKDDKKHHDSDHHRDREPGTSNSLLVSESLRGTDKRRNGPDYSNDIKKRKIEDKDSSHYDSDGDKSDDNLVVDVSNEDPTSPRVSPAHSPRENGIDKSRLLKKDASSSPASTASSGSSTSLKSKEMSLHEKASTPVLKSSTPTPRTDVPTPGTSVTSGLRPGLGKPPSMDPLINQAAAAGLRTPLAVPGPYAGPFGMVPHAGMNGELTSPGAAYASLHNMSPHMSAAAAAAVVAYGRSPMSGFDSPPHMRVPGMPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISQPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISIDDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	NM_001170503.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 1 (tle1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170503.1

ORF Insert Sequence:

ATGTTTCCAC AAAGCAGGCA CCCGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGGTACGTGG CCTTCCAGGT CTACCTCCTA CACAACAGCA GTTACAGGCC 
CAGCATCTAT CCCACAGTCA TGGACCGCCA GTGCCTCTGA CACCACATCC ATCAGGTCTT 
CAACCCCAAG GAATTCCACC TCTGGGAAGT AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT 
CTCAGTGGCC AGTCTCACCT AGCCATGAAA GATGACAAGA AGCACCATGA TTCAGACCAC 
CATAGAGACA GAGAGCCAGG AACAAGTAAT TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT 
ACTGATAAAA GAAGGAATGG TCCTGATTAT TCTAATGACA TAAAAAAAAG AAAGATTGAG 
GACAAGGATT CCAGTCACTA TGACAGTGAT GGAGACAAAA GTGATGATAA CTTGGTTGTG 
GATGTATCCA ATGAGGATCC TACATCTCCT AGAGTGAGTC CTGCTCACTC ACCTAGAGAA 
AACGGAATTG ATAAAAGTAG GTTACTTAAG AAAGATGCTT CAAGCAGTCC CGCATCCACT 
GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC 
AGCACGCCTG TTCTGAAATC AAGCACACCA ACTCCTCGAA CTGATGTGCC GACACCCGGC 
ACAAGTGTGA CGTCAGGGTT GCGACCAGGT CTTGGCAAAC CACCATCTAT GGATCCCCTC 
ATTAACCAAG CTGCTGCTGC TGGCTTGAGA ACACCTTTGG CAGTTCCTGG TCCCTATGCC 
GGTCCATTTG GAATGGTACC ACATGCAGGC ATGAATGGAG AACTGACGAG TCCAGGAGCT 
GCCTATGCTA GCTTGCACAA TATGTCCCCC CATATGAGTG CAGCTGCAGC AGCTGCTGTT 
GTAGCTTATG GACGATCGCC AATGTCTGGA TTTGACTCTC CACCACACAT GCGAGTGCCA 
GGAATGCCTC CAAATTTAGC TGGAATCCCT GGTGGCAAAC CGGCCTATTC GTTTCATGTT 
ACAGCAGATG GTCAAATGCA GCCAGTCCCT TTCCCTCCTG ATGCACTTAT AGGACCAGGC 
ATTCCAAGAC ATGCACGGCA GATAAACACA CTAAATCACG GGGAGGTAGT GTGCGCAGTT 
ACCATCAGTA ACCCCACGAG ACATGTGTAC ACTGGTGGGA AGGGATGTGT TAAAGTCTGG 
GATATCAGCC AACCTGGCAA CAAAAGCCCT GTCTCTCAGC TGGATTGCCT GAACAGAGAT 
AACTACATCA GATCATGTAA ATTGCTGCCT GATGGATGCA CTCTTATTGT GGGAGGGGAA 
GCCAGCACAT TATCCATTTG GGACCTGGCA GCTCCTACTC CACGTATAAA AGCAGAACTG 
ACATCATCTG CTCCAGCCTG CTATGCTCTG GCCATCAGCC CTGATTCCAA GGTCTGCTTC 
TCCTGCTGTA GTGATGGCAA CATTGCAGTC TGGGATTTGC ACAACCAGAC TCTAGTAAGG 
CAGTTCCAGG GCCACACAGA TGGAGCCAGC TGTATTGACA TTTCTAATGA TGGTACCAAG 
CTCTGGACCG GGGGTTTGGA CAACACGGTA AGGTCCTGGG ACCTGCGTGA AGGACGGCAG 
CTCCAGCAGC ATGACTTTAC ATCACAGATC TTCTCTCTTG GGTATTGCCC AACTGGTGAA 
TGGCTTGCTG TTGGAATGGA GAGTAGTAAT GTGGAAGTAC TGCATGTAAA CAAGCCAGAC 
AAGTATCAGC TGCACCTGCA TGAAAGCTGT GTACTCTCAC TAAAATTTGC CTATTGCGGC 
AAATGGTTTG TAAGTACTGG AAAGGATAAT CTTCTTAATG CTTGGCGAAC CCCATATGGA 
GCCAGTATAT TCCAGTCCAA AGAATCCTCA TCTGTACTGA GCTGCGATAT CTCTATCGAT 
GACAAATACA TTGTCACTGG GTCAGGGGAC AAGAAGGCCA CAGTCTATGA AGTCATCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle1 ( XM_012961206.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816660.1 Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30284
Clone ID Related Accession (Same CDS sequence)	XM_012961206.2 , XM_012961206.3
Accession Version	XM_012961206.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin-like enhancer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGGA GGGGCAGAGC TCTGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGGTACGTGG CCTTCCAGGT CTACCTCCTA CACAACAGCA GTTACAGGCC 
CAGCATCTAT CCCACAGTCA TGGACCGCCA GTGCCTCTGA CACCACATCC ATCAGGTCTT 
CAACCCCAAG GAATTCCACC TCTGGGAAGT AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT 
CTCAGTGGCC AGTCTCACCT AGCCATGAAA GATGACAAGA AGCACCATGA TTCAGACCAC 
CATAGAGACA GAGAGCCAGG AACAAGTAAT TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT 
ACTGATAAAA GAAGGAATGG TCCTGATTAT TCTAATGACA TAAAAAAAAG AAAGATTGAG 
GACAAGGATT CCAGTCACTA TGACAGTGAT GGAGACAAAA GTGATGATAA CTTGGTTGTG 
GATGTATCCA ATGAGGATCC TACATCTCCT AGAGTGAGTC CTGCTCACTC ACCTAGAGAA 
AACGGAATTG ATAAAAGTAG GTTACTTAAG AAAGATGCTT CAAGCAGTCC CGCATCCACT 
GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC 
AGCACGCCTG TTCTGAAATC AAGCACACCA ACTCCTCGAA CTGATGTGCC GACACCCGGC 
ACAAGTGTGA CGTCAGGGTT GCGACCAGGT CTTGGCAAAC CACCATCTAT GGATCCCCTC 
ATTAACCAAG CTGCTGCTGC TGGCTTGAGA ACACCTTTGG CAGTTCCTGG TCCCTATGCC 
GGTCCATTTG GAATGGTACC ACATGCAGGC ATGAATGGAG AACTGACGAG TCCAGGAGCT 
GCCTATGCTA GCTTGCACAA TATGTCCCCC CATATGAGTG CAGCTGCAGC AGCTGCTGTT 
GTAGCTTATG GACGATCGCC AATGTCTGGA TTTGACCCTC CACCACACAT GCGAGTGCCA 
GGAATGCCTC CAAATTTAGC TGGAATCCCT GGTGGCAAAC CGGCCTATTC GTTTCATGTT 
ACAGCAGATG GTCAAATGCA GCCAGTCCCT TTCCCTCCTG ATGCACTTAT AGGACCAGGC 
ATTCCAAGAC ATGCACGGCA GATAAACACA CTAAATCACG GGGAGGTAGT GTGCGCAGTT 
ACCATCAGTA ACCCCACGAG ACATGTGTAC ACTGGTGGGA AGGGATGTGT TAAAGTCTGG 
GATATCAGCC AACCTGGCAA CAAAAGCCCT GTCTCTCAGC TGGATTGCCT GAACAGAGAT 
AACTACATCA GATCATGTAA ATTGCTGCCT GATGGATGCA CTCTTATTGT GGGAGGGGAA 
GCCAGCACAT TATCCATTTG GGACCTGGCA GCTCCTACTC CACGTATAAA AGCAGAACTG 
ACATCATCTG CTCCAGCCTG CTATGCTCTG GCCATCAGCC CTGATTCCAA GGTCTGCTTC 
TCCTGCTGTA GTGATGGCAA CATTGCAGTC TGGGATTTGC ACAACCAGAC TCTAGTAAGG 
CAGTTCCAGG GCCACACAGA TGGAGCCAGC TGTATTGACA TTTCTAATGA TGGTACCAAG 
CTCTGGACCG GGGGTTTGGA CAACACGGTA AGGTCCTGGG ACCTGCGTGA AGGACGGCAG 
CTCCAGCAGC ATGACTTTAC ATCACAGATC TTCTCTCTTG GGTATTGCCC AACTGGTGAA 
TGGCTTGCTG TTGGAATGGA GAGTAGTAAT GTGGAAGTAC TGCATGTAAA CAAGCCAGAC 
AAGTATCAGC TGCACCTGCA TGAAAGCTGT GTACTCTCAC TAAAATTTGC CTATTGCGGC 
AAATGGTTTG TAAGTACTGG AAAGGATAAT CTTCTTAATG CTTGGCGAAC CCCATATGGA 
GCCAGTATAT TCCAGTCCAA AGAATCCTCA TCTGTACTGA GCTGCGATAT CTCTATCGAT 
GACAAATACA TTGTCACTGG GTCAGGGGAC AAGAAGGCCA CAGTCTATGA AGTCATCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816660.1
CDS	141..2483
Translation	MCGRGRALTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGVRGLPGLPPTQQQLQAQHLSHSHGPPVPLTPHPSGLQPQGIPPLGSSAGLLALSSALSGQSHLAMKDDKKHHDSDHHRDREPGTSNSLLVSESLRGTDKRRNGPDYSNDIKKRKIEDKDSSHYDSDGDKSDDNLVVDVSNEDPTSPRVSPAHSPRENGIDKSRLLKKDASSSPASTASSGSSTSLKSKEMSLHEKASTPVLKSSTPTPRTDVPTPGTSVTSGLRPGLGKPPSMDPLINQAAAAGLRTPLAVPGPYAGPFGMVPHAGMNGELTSPGAAYASLHNMSPHMSAAAAAAVVAYGRSPMSGFDPPPHMRVPGMPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISQPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISIDDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961206.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961206.2

ORF Insert Sequence:

ATGTGTGGGA GGGGCAGAGC TCTGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGGTACGTGG CCTTCCAGGT CTACCTCCTA CACAACAGCA GTTACAGGCC 
CAGCATCTAT CCCACAGTCA TGGACCGCCA GTGCCTCTGA CACCACATCC ATCAGGTCTT 
CAACCCCAAG GAATTCCACC TCTGGGAAGT AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT 
CTCAGTGGCC AGTCTCACCT AGCCATGAAA GATGACAAGA AGCACCATGA TTCAGACCAC 
CATAGAGACA GAGAGCCAGG AACAAGTAAT TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT 
ACTGATAAAA GAAGGAATGG TCCTGATTAT TCTAATGACA TAAAAAAAAG AAAGATTGAG 
GACAAGGATT CCAGTCACTA TGACAGTGAT GGAGACAAAA GTGATGATAA CTTGGTTGTG 
GATGTATCCA ATGAGGATCC TACATCTCCT AGAGTGAGTC CTGCTCACTC ACCTAGAGAA 
AACGGAATTG ATAAAAGTAG GTTACTTAAG AAAGATGCTT CAAGCAGTCC CGCATCCACT 
GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC 
AGCACGCCTG TTCTGAAATC AAGCACACCA ACTCCTCGAA CTGATGTGCC GACACCCGGC 
ACAAGTGTGA CGTCAGGGTT GCGACCAGGT CTTGGCAAAC CACCATCTAT GGATCCCCTC 
ATTAACCAAG CTGCTGCTGC TGGCTTGAGA ACACCTTTGG CAGTTCCTGG TCCCTATGCC 
GGTCCATTTG GAATGGTACC ACATGCAGGC ATGAATGGAG AACTGACGAG TCCAGGAGCT 
GCCTATGCTA GCTTGCACAA TATGTCCCCC CATATGAGTG CAGCTGCAGC AGCTGCTGTT 
GTAGCTTATG GACGATCGCC AATGTCTGGA TTTGACCCTC CACCACACAT GCGAGTGCCA 
GGAATGCCTC CAAATTTAGC TGGAATCCCT GGTGGCAAAC CGGCCTATTC GTTTCATGTT 
ACAGCAGATG GTCAAATGCA GCCAGTCCCT TTCCCTCCTG ATGCACTTAT AGGACCAGGC 
ATTCCAAGAC ATGCACGGCA GATAAACACA CTAAATCACG GGGAGGTAGT GTGCGCAGTT 
ACCATCAGTA ACCCCACGAG ACATGTGTAC ACTGGTGGGA AGGGATGTGT TAAAGTCTGG 
GATATCAGCC AACCTGGCAA CAAAAGCCCT GTCTCTCAGC TGGATTGCCT GAACAGAGAT 
AACTACATCA GATCATGTAA ATTGCTGCCT GATGGATGCA CTCTTATTGT GGGAGGGGAA 
GCCAGCACAT TATCCATTTG GGACCTGGCA GCTCCTACTC CACGTATAAA AGCAGAACTG 
ACATCATCTG CTCCAGCCTG CTATGCTCTG GCCATCAGCC CTGATTCCAA GGTCTGCTTC 
TCCTGCTGTA GTGATGGCAA CATTGCAGTC TGGGATTTGC ACAACCAGAC TCTAGTAAGG 
CAGTTCCAGG GCCACACAGA TGGAGCCAGC TGTATTGACA TTTCTAATGA TGGTACCAAG 
CTCTGGACCG GGGGTTTGGA CAACACGGTA AGGTCCTGGG ACCTGCGTGA AGGACGGCAG 
CTCCAGCAGC ATGACTTTAC ATCACAGATC TTCTCTCTTG GGTATTGCCC AACTGGTGAA 
TGGCTTGCTG TTGGAATGGA GAGTAGTAAT GTGGAAGTAC TGCATGTAAA CAAGCCAGAC 
AAGTATCAGC TGCACCTGCA TGAAAGCTGT GTACTCTCAC TAAAATTTGC CTATTGCGGC 
AAATGGTTTG TAAGTACTGG AAAGGATAAT CTTCTTAATG CTTGGCGAAC CCCATATGGA 
GCCAGTATAT TCCAGTCCAA AGAATCCTCA TCTGTACTGA GCTGCGATAT CTCTATCGAT 
GACAAATACA TTGTCACTGG GTCAGGGGAC AAGAAGGCCA CAGTCTATGA AGTCATCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle1 ( XM_012961241.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816695.1 Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa30285
Clone ID Related Accession (Same CDS sequence)	XM_012961241.2 , XM_012961241.3
Accession Version	XM_012961241.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin-like enhancer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCCAC AAAGCAGGCA CCCGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGCAACAGCA GTTACAGGCC CAGCATCTAT CCCACAGTCA TGGACCGCCA 
GTGCCTCTGA CACCACATCC ATCAGGTCTT CAACCCCAAG GAATTCCACC TCTGGGAAGT 
AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT CTCAGTGGCC AGTCTCACCT AGCCATGAAA 
GATGACAAGA AGCACCATGA TTCAGACCAC CATAGAGACA GAGAGCCAGG AACAAGTAAT 
TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT ACTGATAAAA GAAGGAATGG TCCTGATTAT 
TCTAATGACA TAAAAAAAAG AAAGATTGAG GACAAGGATT CCAGTCACTA TGACAGTGAT 
GGAGACAAAA GTGATGATAA CTTGGTTGTG GATGTATCCA ATGAGGATCC TACATCTCCT 
AGAGTGAGTC CTGCTCACTC ACCTAGAGAA AACGGAATTG ATAAAAGTAG GTTACTTAAG 
AAAGATGCTT CAAGCAGTCC CGCATCCACT GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA 
TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC AAGCACACCA 
ACTCCTCGAA CTGATGTGCC GACACCCGGC ACAAGTGTGA CGTCAGGGTT GCGACCAGGT 
CTTGGCAAAC CACCATCTAT GGATCCCCTC ATTAACCAAG CTGCTGCTGC TGGCTTGAGA 
ACACCTTTGG CAGTTCCTGG TCCCTATGCC GGTCCATTTG GAATGGTACC ACATGCAGGC 
ATGAATGGAG AACTGACGAG TCCAGGAGCT GCCTATGCTA GCTTGCACAA TATGTCCCCC 
CATATGAGTG CAGCTGCAGC AGCTGCTGTT GTAGCTTATG GACGATCGCC AATGTCTGGA 
TTTGACCCTC CACCACACAT GCGAGTGCCA GGAATGCCTC CAAATTTAGC TGGAATCCCT 
GGTGGCAAAC CGGCCTATTC GTTTCATGTT ACAGCAGATG GTCAAATGCA GCCAGTCCCT 
TTCCCTCCTG ATGCACTTAT AGGACCAGGC ATTCCAAGAC ATGCACGGCA GATAAACACA 
CTAAATCACG GGGAGGTAGT GTGCGCAGTT ACCATCAGTA ACCCCACGAG ACATGTGTAC 
ACTGGTGGGA AGGGATGTGT TAAAGTCTGG GATATCAGCC AACCTGGCAA CAAAAGCCCT 
GTCTCTCAGC TGGATTGCCT GAACAGAGAT AACTACATCA GATCATGTAA ATTGCTGCCT 
GATGGATGCA CTCTTATTGT GGGAGGGGAA GCCAGCACAT TATCCATTTG GGACCTGGCA 
GCTCCTACTC CACGTATAAA AGCAGAACTG ACATCATCTG CTCCAGCCTG CTATGCTCTG 
GCCATCAGCC CTGATTCCAA GGTCTGCTTC TCCTGCTGTA GTGATGGCAA CATTGCAGTC 
TGGGATTTGC ACAACCAGAC TCTAGTAAGG CAGTTCCAGG GCCACACAGA TGGAGCCAGC 
TGTATTGACA TTTCTAATGA TGGTACCAAG CTCTGGACCG GGGGTTTGGA CAACACGGTA 
AGGTCCTGGG ACCTGCGTGA AGGACGGCAG CTCCAGCAGC ATGACTTTAC ATCACAGATC 
TTCTCTCTTG GGTATTGCCC AACTGGTGAA TGGCTTGCTG TTGGAATGGA GAGTAGTAAT 
GTGGAAGTAC TGCATGTAAA CAAGCCAGAC AAGTATCAGC TGCACCTGCA TGAAAGCTGT 
GTACTCTCAC TAAAATTTGC CTATTGCGGC AAATGGTTTG TAAGTACTGG AAAGGATAAT 
CTTCTTAATG CTTGGCGAAC CCCATATGGA GCCAGTATAT TCCAGTCCAA AGAATCCTCA 
TCTGTACTGA GCTGCGATAT CTCTATCGAT GACAAATACA TTGTCACTGG GTCAGGGGAC 
AAGAAGGCCA CAGTCTATGA AGTCATCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816695.1
CDS	868..3180
Translation	MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHSHGPPVPLTPHPSGLQPQGIPPLGSSAGLLALSSALSGQSHLAMKDDKKHHDSDHHRDREPGTSNSLLVSESLRGTDKRRNGPDYSNDIKKRKIEDKDSSHYDSDGDKSDDNLVVDVSNEDPTSPRVSPAHSPRENGIDKSRLLKKDASSSPASTASSGSSTSLKSKEMSLHEKASTPVLKSSTPTPRTDVPTPGTSVTSGLRPGLGKPPSMDPLINQAAAAGLRTPLAVPGPYAGPFGMVPHAGMNGELTSPGAAYASLHNMSPHMSAAAAAAVVAYGRSPMSGFDPPPHMRVPGMPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISQPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISIDDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961241.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961241.2

ORF Insert Sequence:

ATGTTTCCAC AAAGCAGGCA CCCGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGCAACAGCA GTTACAGGCC CAGCATCTAT CCCACAGTCA TGGACCGCCA 
GTGCCTCTGA CACCACATCC ATCAGGTCTT CAACCCCAAG GAATTCCACC TCTGGGAAGT 
AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT CTCAGTGGCC AGTCTCACCT AGCCATGAAA 
GATGACAAGA AGCACCATGA TTCAGACCAC CATAGAGACA GAGAGCCAGG AACAAGTAAT 
TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT ACTGATAAAA GAAGGAATGG TCCTGATTAT 
TCTAATGACA TAAAAAAAAG AAAGATTGAG GACAAGGATT CCAGTCACTA TGACAGTGAT 
GGAGACAAAA GTGATGATAA CTTGGTTGTG GATGTATCCA ATGAGGATCC TACATCTCCT 
AGAGTGAGTC CTGCTCACTC ACCTAGAGAA AACGGAATTG ATAAAAGTAG GTTACTTAAG 
AAAGATGCTT CAAGCAGTCC CGCATCCACT GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA 
TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC AAGCACACCA 
ACTCCTCGAA CTGATGTGCC GACACCCGGC ACAAGTGTGA CGTCAGGGTT GCGACCAGGT 
CTTGGCAAAC CACCATCTAT GGATCCCCTC ATTAACCAAG CTGCTGCTGC TGGCTTGAGA 
ACACCTTTGG CAGTTCCTGG TCCCTATGCC GGTCCATTTG GAATGGTACC ACATGCAGGC 
ATGAATGGAG AACTGACGAG TCCAGGAGCT GCCTATGCTA GCTTGCACAA TATGTCCCCC 
CATATGAGTG CAGCTGCAGC AGCTGCTGTT GTAGCTTATG GACGATCGCC AATGTCTGGA 
TTTGACCCTC CACCACACAT GCGAGTGCCA GGAATGCCTC CAAATTTAGC TGGAATCCCT 
GGTGGCAAAC CGGCCTATTC GTTTCATGTT ACAGCAGATG GTCAAATGCA GCCAGTCCCT 
TTCCCTCCTG ATGCACTTAT AGGACCAGGC ATTCCAAGAC ATGCACGGCA GATAAACACA 
CTAAATCACG GGGAGGTAGT GTGCGCAGTT ACCATCAGTA ACCCCACGAG ACATGTGTAC 
ACTGGTGGGA AGGGATGTGT TAAAGTCTGG GATATCAGCC AACCTGGCAA CAAAAGCCCT 
GTCTCTCAGC TGGATTGCCT GAACAGAGAT AACTACATCA GATCATGTAA ATTGCTGCCT 
GATGGATGCA CTCTTATTGT GGGAGGGGAA GCCAGCACAT TATCCATTTG GGACCTGGCA 
GCTCCTACTC CACGTATAAA AGCAGAACTG ACATCATCTG CTCCAGCCTG CTATGCTCTG 
GCCATCAGCC CTGATTCCAA GGTCTGCTTC TCCTGCTGTA GTGATGGCAA CATTGCAGTC 
TGGGATTTGC ACAACCAGAC TCTAGTAAGG CAGTTCCAGG GCCACACAGA TGGAGCCAGC 
TGTATTGACA TTTCTAATGA TGGTACCAAG CTCTGGACCG GGGGTTTGGA CAACACGGTA 
AGGTCCTGGG ACCTGCGTGA AGGACGGCAG CTCCAGCAGC ATGACTTTAC ATCACAGATC 
TTCTCTCTTG GGTATTGCCC AACTGGTGAA TGGCTTGCTG TTGGAATGGA GAGTAGTAAT 
GTGGAAGTAC TGCATGTAAA CAAGCCAGAC AAGTATCAGC TGCACCTGCA TGAAAGCTGT 
GTACTCTCAC TAAAATTTGC CTATTGCGGC AAATGGTTTG TAAGTACTGG AAAGGATAAT 
CTTCTTAATG CTTGGCGAAC CCCATATGGA GCCAGTATAT TCCAGTCCAA AGAATCCTCA 
TCTGTACTGA GCTGCGATAT CTCTATCGAT GACAAATACA TTGTCACTGG GTCAGGGGAC 
AAGAAGGCCA CAGTCTATGA AGTCATCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle1 ( XM_012961206.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816660.1 Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa30284
Clone ID Related Accession (Same CDS sequence)	XM_012961206.2 , XM_012961206.3
Accession Version	XM_012961206.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin-like enhancer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961206.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGGA GGGGCAGAGC TCTGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGGTACGTGG CCTTCCAGGT CTACCTCCTA CACAACAGCA GTTACAGGCC 
CAGCATCTAT CCCACAGTCA TGGACCGCCA GTGCCTCTGA CACCACATCC ATCAGGTCTT 
CAACCCCAAG GAATTCCACC TCTGGGAAGT AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT 
CTCAGTGGCC AGTCTCACCT AGCCATGAAA GATGACAAGA AGCACCATGA TTCAGACCAC 
CATAGAGACA GAGAGCCAGG AACAAGTAAT TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT 
ACTGATAAAA GAAGGAATGG TCCTGATTAT TCTAATGACA TAAAAAAAAG AAAGATTGAG 
GACAAGGATT CCAGTCACTA TGACAGTGAT GGAGACAAAA GTGATGATAA CTTGGTTGTG 
GATGTATCCA ATGAGGATCC TACATCTCCT AGAGTGAGTC CTGCTCACTC ACCTAGAGAA 
AACGGAATTG ATAAAAGTAG GTTACTTAAG AAAGATGCTT CAAGCAGTCC CGCATCCACT 
GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC 
AGCACGCCTG TTCTGAAATC AAGCACACCA ACTCCTCGAA CTGATGTGCC GACACCCGGC 
ACAAGTGTGA CGTCAGGGTT GCGACCAGGT CTTGGCAAAC CACCATCTAT GGATCCCCTC 
ATTAACCAAG CTGCTGCTGC TGGCTTGAGA ACACCTTTGG CAGTTCCTGG TCCCTATGCC 
GGTCCATTTG GAATGGTACC ACATGCAGGC ATGAATGGAG AACTGACGAG TCCAGGAGCT 
GCCTATGCTA GCTTGCACAA TATGTCCCCC CATATGAGTG CAGCTGCAGC AGCTGCTGTT 
GTAGCTTATG GACGATCGCC AATGTCTGGA TTTGACCCTC CACCACACAT GCGAGTGCCA 
GGAATGCCTC CAAATTTAGC TGGAATCCCT GGTGGCAAAC CGGCCTATTC GTTTCATGTT 
ACAGCAGATG GTCAAATGCA GCCAGTCCCT TTCCCTCCTG ATGCACTTAT AGGACCAGGC 
ATTCCAAGAC ATGCACGGCA GATAAACACA CTAAATCACG GGGAGGTAGT GTGCGCAGTT 
ACCATCAGTA ACCCCACGAG ACATGTGTAC ACTGGTGGGA AGGGATGTGT TAAAGTCTGG 
GATATCAGCC AACCTGGCAA CAAAAGCCCT GTCTCTCAGC TGGATTGCCT GAACAGAGAT 
AACTACATCA GATCATGTAA ATTGCTGCCT GATGGATGCA CTCTTATTGT GGGAGGGGAA 
GCCAGCACAT TATCCATTTG GGACCTGGCA GCTCCTACTC CACGTATAAA AGCAGAACTG 
ACATCATCTG CTCCAGCCTG CTATGCTCTG GCCATCAGCC CTGATTCCAA GGTCTGCTTC 
TCCTGCTGTA GTGATGGCAA CATTGCAGTC TGGGATTTGC ACAACCAGAC TCTAGTAAGG 
CAGTTCCAGG GCCACACAGA TGGAGCCAGC TGTATTGACA TTTCTAATGA TGGTACCAAG 
CTCTGGACCG GGGGTTTGGA CAACACGGTA AGGTCCTGGG ACCTGCGTGA AGGACGGCAG 
CTCCAGCAGC ATGACTTTAC ATCACAGATC TTCTCTCTTG GGTATTGCCC AACTGGTGAA 
TGGCTTGCTG TTGGAATGGA GAGTAGTAAT GTGGAAGTAC TGCATGTAAA CAAGCCAGAC 
AAGTATCAGC TGCACCTGCA TGAAAGCTGT GTACTCTCAC TAAAATTTGC CTATTGCGGC 
AAATGGTTTG TAAGTACTGG AAAGGATAAT CTTCTTAATG CTTGGCGAAC CCCATATGGA 
GCCAGTATAT TCCAGTCCAA AGAATCCTCA TCTGTACTGA GCTGCGATAT CTCTATCGAT 
GACAAATACA TTGTCACTGG GTCAGGGGAC AAGAAGGCCA CAGTCTATGA AGTCATCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816660.1
CDS	120..2462
Translation	MCGRGRALTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGVRGLPGLPPTQQQLQAQHLSHSHGPPVPLTPHPSGLQPQGIPPLGSSAGLLALSSALSGQSHLAMKDDKKHHDSDHHRDREPGTSNSLLVSESLRGTDKRRNGPDYSNDIKKRKIEDKDSSHYDSDGDKSDDNLVVDVSNEDPTSPRVSPAHSPRENGIDKSRLLKKDASSSPASTASSGSSTSLKSKEMSLHEKASTPVLKSSTPTPRTDVPTPGTSVTSGLRPGLGKPPSMDPLINQAAAAGLRTPLAVPGPYAGPFGMVPHAGMNGELTSPGAAYASLHNMSPHMSAAAAAAVVAYGRSPMSGFDPPPHMRVPGMPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISQPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISIDDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961206.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961206.3

ORF Insert Sequence:

ATGTGTGGGA GGGGCAGAGC TCTGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGGTACGTGG CCTTCCAGGT CTACCTCCTA CACAACAGCA GTTACAGGCC 
CAGCATCTAT CCCACAGTCA TGGACCGCCA GTGCCTCTGA CACCACATCC ATCAGGTCTT 
CAACCCCAAG GAATTCCACC TCTGGGAAGT AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT 
CTCAGTGGCC AGTCTCACCT AGCCATGAAA GATGACAAGA AGCACCATGA TTCAGACCAC 
CATAGAGACA GAGAGCCAGG AACAAGTAAT TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT 
ACTGATAAAA GAAGGAATGG TCCTGATTAT TCTAATGACA TAAAAAAAAG AAAGATTGAG 
GACAAGGATT CCAGTCACTA TGACAGTGAT GGAGACAAAA GTGATGATAA CTTGGTTGTG 
GATGTATCCA ATGAGGATCC TACATCTCCT AGAGTGAGTC CTGCTCACTC ACCTAGAGAA 
AACGGAATTG ATAAAAGTAG GTTACTTAAG AAAGATGCTT CAAGCAGTCC CGCATCCACT 
GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC 
AGCACGCCTG TTCTGAAATC AAGCACACCA ACTCCTCGAA CTGATGTGCC GACACCCGGC 
ACAAGTGTGA CGTCAGGGTT GCGACCAGGT CTTGGCAAAC CACCATCTAT GGATCCCCTC 
ATTAACCAAG CTGCTGCTGC TGGCTTGAGA ACACCTTTGG CAGTTCCTGG TCCCTATGCC 
GGTCCATTTG GAATGGTACC ACATGCAGGC ATGAATGGAG AACTGACGAG TCCAGGAGCT 
GCCTATGCTA GCTTGCACAA TATGTCCCCC CATATGAGTG CAGCTGCAGC AGCTGCTGTT 
GTAGCTTATG GACGATCGCC AATGTCTGGA TTTGACCCTC CACCACACAT GCGAGTGCCA 
GGAATGCCTC CAAATTTAGC TGGAATCCCT GGTGGCAAAC CGGCCTATTC GTTTCATGTT 
ACAGCAGATG GTCAAATGCA GCCAGTCCCT TTCCCTCCTG ATGCACTTAT AGGACCAGGC 
ATTCCAAGAC ATGCACGGCA GATAAACACA CTAAATCACG GGGAGGTAGT GTGCGCAGTT 
ACCATCAGTA ACCCCACGAG ACATGTGTAC ACTGGTGGGA AGGGATGTGT TAAAGTCTGG 
GATATCAGCC AACCTGGCAA CAAAAGCCCT GTCTCTCAGC TGGATTGCCT GAACAGAGAT 
AACTACATCA GATCATGTAA ATTGCTGCCT GATGGATGCA CTCTTATTGT GGGAGGGGAA 
GCCAGCACAT TATCCATTTG GGACCTGGCA GCTCCTACTC CACGTATAAA AGCAGAACTG 
ACATCATCTG CTCCAGCCTG CTATGCTCTG GCCATCAGCC CTGATTCCAA GGTCTGCTTC 
TCCTGCTGTA GTGATGGCAA CATTGCAGTC TGGGATTTGC ACAACCAGAC TCTAGTAAGG 
CAGTTCCAGG GCCACACAGA TGGAGCCAGC TGTATTGACA TTTCTAATGA TGGTACCAAG 
CTCTGGACCG GGGGTTTGGA CAACACGGTA AGGTCCTGGG ACCTGCGTGA AGGACGGCAG 
CTCCAGCAGC ATGACTTTAC ATCACAGATC TTCTCTCTTG GGTATTGCCC AACTGGTGAA 
TGGCTTGCTG TTGGAATGGA GAGTAGTAAT GTGGAAGTAC TGCATGTAAA CAAGCCAGAC 
AAGTATCAGC TGCACCTGCA TGAAAGCTGT GTACTCTCAC TAAAATTTGC CTATTGCGGC 
AAATGGTTTG TAAGTACTGG AAAGGATAAT CTTCTTAATG CTTGGCGAAC CCCATATGGA 
GCCAGTATAT TCCAGTCCAA AGAATCCTCA TCTGTACTGA GCTGCGATAT CTCTATCGAT 
GACAAATACA TTGTCACTGG GTCAGGGGAC AAGAAGGCCA CAGTCTATGA AGTCATCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle1 ( XM_012961241.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816695.1 Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa30285
Clone ID Related Accession (Same CDS sequence)	XM_012961241.2 , XM_012961241.3
Accession Version	XM_012961241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin-like enhancer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961241.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCCAC AAAGCAGGCA CCCGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGCAACAGCA GTTACAGGCC CAGCATCTAT CCCACAGTCA TGGACCGCCA 
GTGCCTCTGA CACCACATCC ATCAGGTCTT CAACCCCAAG GAATTCCACC TCTGGGAAGT 
AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT CTCAGTGGCC AGTCTCACCT AGCCATGAAA 
GATGACAAGA AGCACCATGA TTCAGACCAC CATAGAGACA GAGAGCCAGG AACAAGTAAT 
TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT ACTGATAAAA GAAGGAATGG TCCTGATTAT 
TCTAATGACA TAAAAAAAAG AAAGATTGAG GACAAGGATT CCAGTCACTA TGACAGTGAT 
GGAGACAAAA GTGATGATAA CTTGGTTGTG GATGTATCCA ATGAGGATCC TACATCTCCT 
AGAGTGAGTC CTGCTCACTC ACCTAGAGAA AACGGAATTG ATAAAAGTAG GTTACTTAAG 
AAAGATGCTT CAAGCAGTCC CGCATCCACT GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA 
TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC AAGCACACCA 
ACTCCTCGAA CTGATGTGCC GACACCCGGC ACAAGTGTGA CGTCAGGGTT GCGACCAGGT 
CTTGGCAAAC CACCATCTAT GGATCCCCTC ATTAACCAAG CTGCTGCTGC TGGCTTGAGA 
ACACCTTTGG CAGTTCCTGG TCCCTATGCC GGTCCATTTG GAATGGTACC ACATGCAGGC 
ATGAATGGAG AACTGACGAG TCCAGGAGCT GCCTATGCTA GCTTGCACAA TATGTCCCCC 
CATATGAGTG CAGCTGCAGC AGCTGCTGTT GTAGCTTATG GACGATCGCC AATGTCTGGA 
TTTGACCCTC CACCACACAT GCGAGTGCCA GGAATGCCTC CAAATTTAGC TGGAATCCCT 
GGTGGCAAAC CGGCCTATTC GTTTCATGTT ACAGCAGATG GTCAAATGCA GCCAGTCCCT 
TTCCCTCCTG ATGCACTTAT AGGACCAGGC ATTCCAAGAC ATGCACGGCA GATAAACACA 
CTAAATCACG GGGAGGTAGT GTGCGCAGTT ACCATCAGTA ACCCCACGAG ACATGTGTAC 
ACTGGTGGGA AGGGATGTGT TAAAGTCTGG GATATCAGCC AACCTGGCAA CAAAAGCCCT 
GTCTCTCAGC TGGATTGCCT GAACAGAGAT AACTACATCA GATCATGTAA ATTGCTGCCT 
GATGGATGCA CTCTTATTGT GGGAGGGGAA GCCAGCACAT TATCCATTTG GGACCTGGCA 
GCTCCTACTC CACGTATAAA AGCAGAACTG ACATCATCTG CTCCAGCCTG CTATGCTCTG 
GCCATCAGCC CTGATTCCAA GGTCTGCTTC TCCTGCTGTA GTGATGGCAA CATTGCAGTC 
TGGGATTTGC ACAACCAGAC TCTAGTAAGG CAGTTCCAGG GCCACACAGA TGGAGCCAGC 
TGTATTGACA TTTCTAATGA TGGTACCAAG CTCTGGACCG GGGGTTTGGA CAACACGGTA 
AGGTCCTGGG ACCTGCGTGA AGGACGGCAG CTCCAGCAGC ATGACTTTAC ATCACAGATC 
TTCTCTCTTG GGTATTGCCC AACTGGTGAA TGGCTTGCTG TTGGAATGGA GAGTAGTAAT 
GTGGAAGTAC TGCATGTAAA CAAGCCAGAC AAGTATCAGC TGCACCTGCA TGAAAGCTGT 
GTACTCTCAC TAAAATTTGC CTATTGCGGC AAATGGTTTG TAAGTACTGG AAAGGATAAT 
CTTCTTAATG CTTGGCGAAC CCCATATGGA GCCAGTATAT TCCAGTCCAA AGAATCCTCA 
TCTGTACTGA GCTGCGATAT CTCTATCGAT GACAAATACA TTGTCACTGG GTCAGGGGAC 
AAGAAGGCCA CAGTCTATGA AGTCATCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816695.1
CDS	868..3180
Translation	MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHSHGPPVPLTPHPSGLQPQGIPPLGSSAGLLALSSALSGQSHLAMKDDKKHHDSDHHRDREPGTSNSLLVSESLRGTDKRRNGPDYSNDIKKRKIEDKDSSHYDSDGDKSDDNLVVDVSNEDPTSPRVSPAHSPRENGIDKSRLLKKDASSSPASTASSGSSTSLKSKEMSLHEKASTPVLKSSTPTPRTDVPTPGTSVTSGLRPGLGKPPSMDPLINQAAAAGLRTPLAVPGPYAGPFGMVPHAGMNGELTSPGAAYASLHNMSPHMSAAAAAAVVAYGRSPMSGFDPPPHMRVPGMPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISQPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISIDDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961241.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961241.3

ORF Insert Sequence:

ATGTTTCCAC AAAGCAGGCA CCCGACGCCG CATCAGGCTG CAGGCCAGCC ATTCAAATTC 
ACTATCCCTG AGTCCTTGGA CCGAATCAAA GAAGAATTCC AGTTTCTTCA AGCTCAGTAT 
CACAGCCTTA AGTTGGAATG TGAAAAATTG GCAAGCGAAA AGACAGAGAT GCAGAGGCAC 
TATGTCATGT ATTATGAGAT GTCCTATGGA CTTAATATTG AAATGCACAA ACAGACCGAA 
ATTGCCAAAC GATTAAACAC CATTTGTGCA CAAGTCATCC CATTTCTTTC TCAGGAACAC 
CAGCAACAGG TGGCTCAAGC TGTAGAGCGT GCCAAACAAG TGACAATGGC AGAGCTAAAT 
GCCATCATCG GGCAACAGCA GTTACAGGCC CAGCATCTAT CCCACAGTCA TGGACCGCCA 
GTGCCTCTGA CACCACATCC ATCAGGTCTT CAACCCCAAG GAATTCCACC TCTGGGAAGT 
AGTGCCGGCC TTCTTGCCCT CTCTAGTGCT CTCAGTGGCC AGTCTCACCT AGCCATGAAA 
GATGACAAGA AGCACCATGA TTCAGACCAC CATAGAGACA GAGAGCCAGG AACAAGTAAT 
TCTCTCTTGG TTTCGGAAAG TCTGCGAGGT ACTGATAAAA GAAGGAATGG TCCTGATTAT 
TCTAATGACA TAAAAAAAAG AAAGATTGAG GACAAGGATT CCAGTCACTA TGACAGTGAT 
GGAGACAAAA GTGATGATAA CTTGGTTGTG GATGTATCCA ATGAGGATCC TACATCTCCT 
AGAGTGAGTC CTGCTCACTC ACCTAGAGAA AACGGAATTG ATAAAAGTAG GTTACTTAAG 
AAAGATGCTT CAAGCAGTCC CGCATCCACT GCTTCTTCAG GAAGTTCAAC ATCTTTAAAA 
TCTAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC AAGCACACCA 
ACTCCTCGAA CTGATGTGCC GACACCCGGC ACAAGTGTGA CGTCAGGGTT GCGACCAGGT 
CTTGGCAAAC CACCATCTAT GGATCCCCTC ATTAACCAAG CTGCTGCTGC TGGCTTGAGA 
ACACCTTTGG CAGTTCCTGG TCCCTATGCC GGTCCATTTG GAATGGTACC ACATGCAGGC 
ATGAATGGAG AACTGACGAG TCCAGGAGCT GCCTATGCTA GCTTGCACAA TATGTCCCCC 
CATATGAGTG CAGCTGCAGC AGCTGCTGTT GTAGCTTATG GACGATCGCC AATGTCTGGA 
TTTGACCCTC CACCACACAT GCGAGTGCCA GGAATGCCTC CAAATTTAGC TGGAATCCCT 
GGTGGCAAAC CGGCCTATTC GTTTCATGTT ACAGCAGATG GTCAAATGCA GCCAGTCCCT 
TTCCCTCCTG ATGCACTTAT AGGACCAGGC ATTCCAAGAC ATGCACGGCA GATAAACACA 
CTAAATCACG GGGAGGTAGT GTGCGCAGTT ACCATCAGTA ACCCCACGAG ACATGTGTAC 
ACTGGTGGGA AGGGATGTGT TAAAGTCTGG GATATCAGCC AACCTGGCAA CAAAAGCCCT 
GTCTCTCAGC TGGATTGCCT GAACAGAGAT AACTACATCA GATCATGTAA ATTGCTGCCT 
GATGGATGCA CTCTTATTGT GGGAGGGGAA GCCAGCACAT TATCCATTTG GGACCTGGCA 
GCTCCTACTC CACGTATAAA AGCAGAACTG ACATCATCTG CTCCAGCCTG CTATGCTCTG 
GCCATCAGCC CTGATTCCAA GGTCTGCTTC TCCTGCTGTA GTGATGGCAA CATTGCAGTC 
TGGGATTTGC ACAACCAGAC TCTAGTAAGG CAGTTCCAGG GCCACACAGA TGGAGCCAGC 
TGTATTGACA TTTCTAATGA TGGTACCAAG CTCTGGACCG GGGGTTTGGA CAACACGGTA 
AGGTCCTGGG ACCTGCGTGA AGGACGGCAG CTCCAGCAGC ATGACTTTAC ATCACAGATC 
TTCTCTCTTG GGTATTGCCC AACTGGTGAA TGGCTTGCTG TTGGAATGGA GAGTAGTAAT 
GTGGAAGTAC TGCATGTAAA CAAGCCAGAC AAGTATCAGC TGCACCTGCA TGAAAGCTGT 
GTACTCTCAC TAAAATTTGC CTATTGCGGC AAATGGTTTG TAAGTACTGG AAAGGATAAT 
CTTCTTAATG CTTGGCGAAC CCCATATGGA GCCAGTATAT TCCAGTCCAA AGAATCCTCA 
TCTGTACTGA GCTGCGATAT CTCTATCGAT GACAAATACA TTGTCACTGG GTCAGGGGAC 
AAGAAGGCCA CAGTCTATGA AGTCATCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle1 ( XM_018092648.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948137.1 Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X3, mRNA.

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CloneID	OXa41633
Clone ID Related Accession (Same CDS sequence)	XM_018092648.1
Accession Version	XM_018092648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin-like enhancer protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGGC ACTATGTCAT GTATTATGAG ATGTCCTATG GACTTAATAT TGAAATGCAC 
AAACAGACCG AAATTGCCAA ACGATTAAAC ACCATTTGTG CACAAGTCAT CCCATTTCTT 
TCTCAGGAAC ACCAGCAACA GGTGGCTCAA GCTGTAGAGC GTGCCAAACA AGTGACAATG 
GCAGAGCTAA ATGCCATCAT CGGGGTACGT GGCCTTCCAG GTCTACCTCC TACACAACAG 
CAGTTACAGG CCCAGCATCT ATCCCACAGT CATGGACCGC CAGTGCCTCT GACACCACAT 
CCATCAGGTC TTCAACCCCA AGGAATTCCA CCTCTGGGAA GTAGTGCCGG CCTTCTTGCC 
CTCTCTAGTG CTCTCAGTGG CCAGTCTCAC CTAGCCATGA AAGATGACAA GAAGCACCAT 
GATTCAGACC ACCATAGAGA CAGAGAGCCA GGAACAAGTA ATTCTCTCTT GGTTTCGGAA 
AGTCTGCGAG GTACTGATAA AAGAAGGAAT GGTCCTGATT ATTCTAATGA CATAAAAAAA 
AGAAAGATTG AGGACAAGGA TTCCAGTCAC TATGACAGTG ATGGAGACAA AAGTGATGAT 
AACTTGGTTG TGGATGTATC CAATGAGGAT CCTACATCTC CTAGAGTGAG TCCTGCTCAC 
TCACCTAGAG AAAACGGAAT TGATAAAAGT AGGTTACTTA AGAAAGATGC TTCAAGCAGT 
CCCGCATCCA CTGCTTCTTC AGGAAGTTCA ACATCTTTAA AATCTAAAGA AATGAGCTTG 
CATGAAAAAG CCAGCACGCC TGTTCTGAAA TCAAGCACAC CAACTCCTCG AACTGATGTG 
CCGACACCCG GCACAAGTGT GACGTCAGGG TTGCGACCAG GTCTTGGCAA ACCACCATCT 
ATGGATCCCC TCATTAACCA AGCTGCTGCT GCTGGCTTGA GAACACCTTT GGCAGTTCCT 
GGTCCCTATG CCGGTCCATT TGGAATGGTA CCACATGCAG GCATGAATGG AGAACTGACG 
AGTCCAGGAG CTGCCTATGC TAGCTTGCAC AATATGTCCC CCCATATGAG TGCAGCTGCA 
GCAGCTGCTG TTGTAGCTTA TGGACGATCG CCAATGTCTG GATTTGACCC TCCACCACAC 
ATGCGAGTGC CAGGAATGCC TCCAAATTTA GCTGGAATCC CTGGTGGCAA ACCGGCCTAT 
TCGTTTCATG TTACAGCAGA TGGTCAAATG CAGCCAGTCC CTTTCCCTCC TGATGCACTT 
ATAGGACCAG GCATTCCAAG ACATGCACGG CAGATAAACA CACTAAATCA CGGGGAGGTA 
GTGTGCGCAG TTACCATCAG TAACCCCACG AGACATGTGT ACACTGGTGG GAAGGGATGT 
GTTAAAGTCT GGGATATCAG CCAACCTGGC AACAAAAGCC CTGTCTCTCA GCTGGATTGC 
CTGAACAGAG ATAACTACAT CAGATCATGT AAATTGCTGC CTGATGGATG CACTCTTATT 
GTGGGAGGGG AAGCCAGCAC ATTATCCATT TGGGACCTGG CAGCTCCTAC TCCACGTATA 
AAAGCAGAAC TGACATCATC TGCTCCAGCC TGCTATGCTC TGGCCATCAG CCCTGATTCC 
AAGGTCTGCT TCTCCTGCTG TAGTGATGGC AACATTGCAG TCTGGGATTT GCACAACCAG 
ACTCTAGTAA GGCAGTTCCA GGGCCACACA GATGGAGCCA GCTGTATTGA CATTTCTAAT 
GATGGTACCA AGCTCTGGAC CGGGGGTTTG GACAACACGG TAAGGTCCTG GGACCTGCGT 
GAAGGACGGC AGCTCCAGCA GCATGACTTT ACATCACAGA TCTTCTCTCT TGGGTATTGC 
CCAACTGGTG AATGGCTTGC TGTTGGAATG GAGAGTAGTA ATGTGGAAGT ACTGCATGTA 
AACAAGCCAG ACAAGTATCA GCTGCACCTG CATGAAAGCT GTGTACTCTC ACTAAAATTT 
GCCTATTGCG GCAAATGGTT TGTAAGTACT GGAAAGGATA ATCTTCTTAA TGCTTGGCGA 
ACCCCATATG GAGCCAGTAT ATTCCAGTCC AAAGAATCCT CATCTGTACT GAGCTGCGAT 
ATCTCTATCG ATGACAAATA CATTGTCACT GGGTCAGGGG ACAAGAAGGC CACAGTCTAT 
GAAGTCATCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948137.1
CDS	1060..3234
Translation	MQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGVRGLPGLPPTQQQLQAQHLSHSHGPPVPLTPHPSGLQPQGIPPLGSSAGLLALSSALSGQSHLAMKDDKKHHDSDHHRDREPGTSNSLLVSESLRGTDKRRNGPDYSNDIKKRKIEDKDSSHYDSDGDKSDDNLVVDVSNEDPTSPRVSPAHSPRENGIDKSRLLKKDASSSPASTASSGSSTSLKSKEMSLHEKASTPVLKSSTPTPRTDVPTPGTSVTSGLRPGLGKPPSMDPLINQAAAAGLRTPLAVPGPYAGPFGMVPHAGMNGELTSPGAAYASLHNMSPHMSAAAAAAVVAYGRSPMSGFDPPPHMRVPGMPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISQPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISIDDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_018092648.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 1 (tle1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092648.1

ORF Insert Sequence:

ATGCAGAGGC ACTATGTCAT GTATTATGAG ATGTCCTATG GACTTAATAT TGAAATGCAC 
AAACAGACCG AAATTGCCAA ACGATTAAAC ACCATTTGTG CACAAGTCAT CCCATTTCTT 
TCTCAGGAAC ACCAGCAACA GGTGGCTCAA GCTGTAGAGC GTGCCAAACA AGTGACAATG 
GCAGAGCTAA ATGCCATCAT CGGGGTACGT GGCCTTCCAG GTCTACCTCC TACACAACAG 
CAGTTACAGG CCCAGCATCT ATCCCACAGT CATGGACCGC CAGTGCCTCT GACACCACAT 
CCATCAGGTC TTCAACCCCA AGGAATTCCA CCTCTGGGAA GTAGTGCCGG CCTTCTTGCC 
CTCTCTAGTG CTCTCAGTGG CCAGTCTCAC CTAGCCATGA AAGATGACAA GAAGCACCAT 
GATTCAGACC ACCATAGAGA CAGAGAGCCA GGAACAAGTA ATTCTCTCTT GGTTTCGGAA 
AGTCTGCGAG GTACTGATAA AAGAAGGAAT GGTCCTGATT ATTCTAATGA CATAAAAAAA 
AGAAAGATTG AGGACAAGGA TTCCAGTCAC TATGACAGTG ATGGAGACAA AAGTGATGAT 
AACTTGGTTG TGGATGTATC CAATGAGGAT CCTACATCTC CTAGAGTGAG TCCTGCTCAC 
TCACCTAGAG AAAACGGAAT TGATAAAAGT AGGTTACTTA AGAAAGATGC TTCAAGCAGT 
CCCGCATCCA CTGCTTCTTC AGGAAGTTCA ACATCTTTAA AATCTAAAGA AATGAGCTTG 
CATGAAAAAG CCAGCACGCC TGTTCTGAAA TCAAGCACAC CAACTCCTCG AACTGATGTG 
CCGACACCCG GCACAAGTGT GACGTCAGGG TTGCGACCAG GTCTTGGCAA ACCACCATCT 
ATGGATCCCC TCATTAACCA AGCTGCTGCT GCTGGCTTGA GAACACCTTT GGCAGTTCCT 
GGTCCCTATG CCGGTCCATT TGGAATGGTA CCACATGCAG GCATGAATGG AGAACTGACG 
AGTCCAGGAG CTGCCTATGC TAGCTTGCAC AATATGTCCC CCCATATGAG TGCAGCTGCA 
GCAGCTGCTG TTGTAGCTTA TGGACGATCG CCAATGTCTG GATTTGACCC TCCACCACAC 
ATGCGAGTGC CAGGAATGCC TCCAAATTTA GCTGGAATCC CTGGTGGCAA ACCGGCCTAT 
TCGTTTCATG TTACAGCAGA TGGTCAAATG CAGCCAGTCC CTTTCCCTCC TGATGCACTT 
ATAGGACCAG GCATTCCAAG ACATGCACGG CAGATAAACA CACTAAATCA CGGGGAGGTA 
GTGTGCGCAG TTACCATCAG TAACCCCACG AGACATGTGT ACACTGGTGG GAAGGGATGT 
GTTAAAGTCT GGGATATCAG CCAACCTGGC AACAAAAGCC CTGTCTCTCA GCTGGATTGC 
CTGAACAGAG ATAACTACAT CAGATCATGT AAATTGCTGC CTGATGGATG CACTCTTATT 
GTGGGAGGGG AAGCCAGCAC ATTATCCATT TGGGACCTGG CAGCTCCTAC TCCACGTATA 
AAAGCAGAAC TGACATCATC TGCTCCAGCC TGCTATGCTC TGGCCATCAG CCCTGATTCC 
AAGGTCTGCT TCTCCTGCTG TAGTGATGGC AACATTGCAG TCTGGGATTT GCACAACCAG 
ACTCTAGTAA GGCAGTTCCA GGGCCACACA GATGGAGCCA GCTGTATTGA CATTTCTAAT 
GATGGTACCA AGCTCTGGAC CGGGGGTTTG GACAACACGG TAAGGTCCTG GGACCTGCGT 
GAAGGACGGC AGCTCCAGCA GCATGACTTT ACATCACAGA TCTTCTCTCT TGGGTATTGC 
CCAACTGGTG AATGGCTTGC TGTTGGAATG GAGAGTAGTA ATGTGGAAGT ACTGCATGTA 
AACAAGCCAG ACAAGTATCA GCTGCACCTG CATGAAAGCT GTGTACTCTC ACTAAAATTT 
GCCTATTGCG GCAAATGGTT TGTAAGTACT GGAAAGGATA ATCTTCTTAA TGCTTGGCGA 
ACCCCATATG GAGCCAGTAT ATTCCAGTCC AAAGAATCCT CATCTGTACT GAGCTGCGAT 
ATCTCTATCG ATGACAAATA CATTGTCACT GGGTCAGGGG ACAAGAAGGC CACAGTCTAT 
GAAGTCATCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Occupancy of tissue-specific cis-regulatory modules by Otx2 and TLE/Groucho for embryonic head specification.
Nature communications54322(2014 Jul)
Yasuoka Y,Suzuki Y,Takahashi S,Someya H,Sudou N,Haramoto Y,Cho KW,Asashima M,Sugano S,Taira M

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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tle1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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