fgf8.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	fgf8.L
Entrez Gene ID	399183
Full Name	fibroblast growth factor 8 L homeolog
Synonyms	AIGF,FGF-8,Xfgf-8,Xfgf8,fgf-8b,fgf8,fgf8a,fgf8b
General protein information	Preferred Names fibroblast growth factor 8 L homeolog Names fibroblast growth factor 8 L homeolog fibroblast growth factor 8 (androgen-induced) fibroblast growth factor-8 fibroblast growth factor-8b
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001090435.1	NP_001083904.1	fibroblast growth factor 8 L homeolog precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Regulation of XFGF8 gene expression through SRY (sex-determining region Y)-box 2 in developing Xenopus embryos.
Kim YH, Shin JY, Na W, Kim J, Ju BG, Kim WS
Reproduction, fertility, and development24(6)769-77(2012)

Tsukushi modulates Xnr2, FGF and BMP signaling: regulation of Xenopus germ layer formation.
Morris SA, Almeida AD, Tanaka H, Ohta K, Ohnuma S
PloS one2(10)e1004(2007 Oct)

FGF8 spliceforms mediate early mesoderm and posterior neural tissue formation in Xenopus.
Fletcher RB, Baker JC, Harland RM
Development (Cambridge, England)133(9)1703-14(2006 May)

FGF8, Wnt8 and Myf5 are target genes of Tbx6 during anteroposterior specification in Xenopus embryo.
Li HY, Bourdelas A, Carron C, Gomez C, Boucaut JC, Shi DL
Developmental biology290(2)470-81(2006 Feb)

Isolation of Xenopus FGF-8b and comparison with FGF-8a.
Shim S, Bae N, Park SY, Kim WS, Han JK
Molecules and cells19(3)310-7(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest that SOX2 directly upregulates FGF8 gene expression in the early embryonic development of Xenopus.
Title: Regulation of XFGF8 gene expression through SRY (sex-determining region Y)-box 2 in developing Xenopus embryos.

Tsukushi regulates Xnr2, FGF and BMP signaling in Xenopus germ layer formation
Title: Tsukushi modulates Xnr2, FGF and BMP signaling: regulation of Xenopus germ layer formation.

FGF-8b spliceform mediates early mesoderm and posterior neural tissue formation in Xenopus.
Title: FGF8 spliceforms mediate early mesoderm and posterior neural tissue formation in Xenopus.

FGF8, Wnt8 and Myf5 are target genes of Tbx6 during anteroposterior specification in Xenopus embryo
Title: FGF8, Wnt8 and Myf5 are target genes of Tbx6 during anteroposterior specification in Xenopus embryo.

cloning & expression of XFGF-8b; XFGF-8b & XFGF-8a are expressed in anterior neural regions of the early embryo & in the apical ectodermal ridge of limb buds & tips of growing digits in larval stages; XFGF-8b is more abundant throughout early development
Title: Isolation of Xenopus FGF-8b and comparison with FGF-8a.

The following fgf8.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fgf8.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf05449	NM_001090435.1 Latest version!	Xenopus laevis fibroblast growth factor 8 L homeolog (fgf8.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fgf8.L ( NM_001090435.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001083904.1 Xenopus laevis fibroblast growth factor 8 L homeolog (fgf8.L), mRNA.

Price & Availability↑

CloneID	OAf05449
Clone ID Related Accession (Same CDS sequence)	NM_001090435.1
Accession Version	NM_001090435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-27
Organism	Xenopus laevis(African clawed frog)
Product	fibroblast growth factor 8 L homeolog precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF461177.1. On Jul 25, 2007 this sequence version replaced NM_001090436.1. ##Evidence-Data-START## Transcript exon combination :: AF461177.1 [ECO:0000332] ##Evidence-Data-END##

ATGAACTACA TCACCTCCAT CCTGGGCTAT CTGTTACTGC ACCTGTTTGT CATCTGCCTA 
CAAGCCCAGG TAACTGTTCA GTCCCCACCT AATTTTACAC AGCATGTGAG GGAGCAGAGC 
CTGGTGACCG ACCAACTAAG CCGACGGCTC ATCCGAACCT ACCAGTTGTA CAGCCGGACC 
AGCGGCAAGC ATGTGCAAAT CCTGGCGAAC AAGAAGATTA ACGCCATGGC AGAAGACGGC 
GACCCACACG CCAAGTTAAT CGTGGAAACA GATACGTTTG GAAGCAGAGT TCGCATTAAA 
GGTGCGGAGA CTGGTTACTA CATCTGCATG AACAAAAAAG GGAAGCTGAT TGGGAAGACT 
AATGGAAGGG GCAAAGACTG CGTCTTCTCG GAAATTGTCC TTGAAAACAA CTACACAGCT 
CTGCAGAATG TCAAGTACGA AGGCTGGTTT ATGGCTTTCA CAAGAAGGGG TCGCCCAAGG 
AAAGGGTCGA AGACAAGGCA ACATCAAAGA GAAGTCCACT TCATGAAGAG GTTGCCAAAG 
GGACACCACA CCACAGAACC TCATAAACGT TTTGAGTTTA TTAATTACCC TTTCAATAGA 
AGAAGTAAAA GAACTCGATA TTCAAGTTCT CGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001083904.1
CDS	703..1338
Translation	MNYITSILGYLLLHLFVICLQAQVTVQSPPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQILANKKINAMAEDGDPHAKLIVETDTFGSRVRIKGAETGYYICMNKKGKLIGKTNGRGKDCVFSEIVLENNYTALQNVKYEGWFMAFTRRGRPRKGSKTRQHQREVHFMKRLPKGHHTTEPHKRFEFINYPFNRRSKRTRYSSSR

Target ORF information:

RefSeq Version	NM_001090435.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis fibroblast growth factor 8 L homeolog (fgf8.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001090435.1

ORF Insert Sequence:

ATGAACTACA TCACCTCCAT CCTGGGCTAT CTGTTACTGC ACCTGTTTGT CATCTGCCTA 
CAAGCCCAGG TAACTGTTCA GTCCCCACCT AATTTTACAC AGCATGTGAG GGAGCAGAGC 
CTGGTGACCG ACCAACTAAG CCGACGGCTC ATCCGAACCT ACCAGTTGTA CAGCCGGACC 
AGCGGCAAGC ATGTGCAAAT CCTGGCGAAC AAGAAGATTA ACGCCATGGC AGAAGACGGC 
GACCCACACG CCAAGTTAAT CGTGGAAACA GATACGTTTG GAAGCAGAGT TCGCATTAAA 
GGTGCGGAGA CTGGTTACTA CATCTGCATG AACAAAAAAG GGAAGCTGAT TGGGAAGACT 
AATGGAAGGG GCAAAGACTG CGTCTTCTCG GAAATTGTCC TTGAAAACAA CTACACAGCT 
CTGCAGAATG TCAAGTACGA AGGCTGGTTT ATGGCTTTCA CAAGAAGGGG TCGCCCAAGG 
AAAGGGTCGA AGACAAGGCA ACATCAAAGA GAAGTCCACT TCATGAAGAG GTTGCCAAAG 
GGACACCACA CCACAGAACC TCATAAACGT TTTGAGTTTA TTAATTACCC TTTCAATAGA 
AGAAGTAAAA GAACTCGATA TTCAAGTTCT CGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of XFGF8 gene expression through SRY (sex-determining region Y)-box 2 in developing Xenopus embryos.
Reproduction, fertility, and development24(6)769-77(2012)
Kim YH,Shin JY,Na W,Kim J,Ju BG,Kim WS

Tsukushi modulates Xnr2, FGF and BMP signaling: regulation of Xenopus germ layer formation.
PloS one2(10)e1004(2007 Oct)
Morris SA,Almeida AD,Tanaka H,Ohta K,Ohnuma S

FGF8 spliceforms mediate early mesoderm and posterior neural tissue formation in Xenopus.
Development (Cambridge, England)133(9)1703-14(2006 May)
Fletcher RB,Baker JC,Harland RM

FGF8, Wnt8 and Myf5 are target genes of Tbx6 during anteroposterior specification in Xenopus embryo.
Developmental biology290(2)470-81(2006 Feb)
Li HY,Bourdelas A,Carron C,Gomez C,Boucaut JC,Shi DL

Isolation of Xenopus FGF-8b and comparison with FGF-8a.
Molecules and cells19(3)310-7(2005 Jun)
Shim S,Bae N,Park SY,Kim WS,Han JK