BUB1 cDNA ORF clone, Vicugna pacos(alpaca)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1
Entrez Gene ID	102533176
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Gene Type	protein-coding
Organism	Vicugna pacos(alpaca)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015238810.1	XP_015094296.1	mitotic checkpoint serine/threonine-protein kinase BUB1
XM_015238810.2	XP_015094296.1	mitotic checkpoint serine/threonine-protein kinase BUB1

Pathways from BioSystems
Gene Ontology

The following BUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OVi39394	XM_015238810.2 Latest version!	Vicugna pacos BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OVi39394	XM_015238810.1	Vicugna pacos BUB1, mitotic checkpoint serine/threonine kinase (BUB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1 ( XM_015238810.1 ) cDNA ORF clone, Vicugna pacos(alpaca) -> XP_015094296.1 Vicugna pacos BUB1, mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Price & Availability↑

CloneID	OVi39394
Clone ID Related Accession (Same CDS sequence)	XM_015238810.1 , XM_015238810.2
Accession Version	XM_015238810.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-28
Organism	Vicugna pacos(alpaca)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005882742.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vicugna pacos Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT CTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGATCCAC TTGGTGAATG GGAAAGTTAC ATGCAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ATTTGACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATACCACA ATGACCCCAG GTTCATCAAT TATTGTTTAA AATTTGCCGA GTGCAACAGT 
GACCTGCATC AGTTTTTTGA GTTTCTGTAC AACCACGGCA TCGGGACGCA GTCGGCTCCC 
CTGTACGTAG CCTGGGCTGG GCACCTGGAA GGCCGGGGGC AGTGGCAGCA CGCCAGCGCT 
GTTTTCCGGA GAGGGATTCA AAACCAGGCC GAACCCGGGG AGCTGCTGCA ACAGCAATAC 
AGGTTGTTTC AGACCCGTCT CACCGAAACC CATGTGCCAA CTCAAGCGAG AACCTCAGAA 
TCTCTGCATG ATGCTCAGGT TTTAAACCAA ATGGTAACAA CACAATCAAA TCCAGGAAAT 
AACTCAGCCT GCATTTCTAA GAATCAGGAT TCAGAACTTT CTGGAGTAAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAA GGAGCAAAGG GTGATCGTGA TTTCTAAGTC AGGATACTCC 
GCACACTCAT CTTTGGCAGC CGCAGCTGAC GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GTGGAGAATC AGAATTTTCC TTTGAAGAAC TGAGAGCACA GAAATACAAT 
CAGCGGAGAA AGCACGAACA GTGGGTAAAC GAAGACAGGC ATTATATGAG AAGGAAAGAA 
GCAAATGCTT TTGAAGAGGA ACTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
TACCAAGTGG TGGAGACGTC GCGGGAGAAC CCACCCTCTT CCCAGGAGAG ATCTGAGGTT 
AATCGCGCCC GTCTGGGGCC ACATGCAGGC TCCCAGCAGG AAGTGAGAGC ACCGAGTCCT 
CCAGCCATCG ATCAGCAGGC CTCAAAGAGC GCGGGAGAGG AACCGCAAGA GGCGCCTCCT 
GTTGTCCCTC TGGCGGCAAA TGCTGTCACT GCTGCTTTGG TGTCCCCAGC CGTCAGCCAG 
AGTGTAGCTC CCCCGGTTTC GGTTTCTCTG ACAGCCCAGC CCATGACAGA CTCCCTGTTT 
ACAGCGGCCG GCAAAGATGC CAGGTGTGTG AATAAAAATA ATCATGAATT CAAGCCTCAG 
AGTGCAGCAG AGCTGAAAGA AGGATGTGAA ACACATAAGG TTGTTAACAC AAGTTCTTTT 
CACACAACTC CAAACACATC ACTGGGACTG GTTCAGGCCA CACCATCCAA AGTGCAGCCG 
TCACCCACTG TGCACACCAA AGAAGCACTG GGCTTCATCA TGAATATGTT TCAGGCTCCT 
ACTCTTCCTG ACATTTCTGA TGACAAAGAT GAATGGCCAT CTCTAGATCA AAATGAAGAC 
GCATTTGAAG CCCAGTTTCA AAAAAATGCA AGTTCTTCCG GGGCTTGGGG AGTCAACAAG 
ATTATCTCTT CTTTGTCATC TGCTTTTCCT GTATTTGAAG ATGGAAACAA AGAAAATTAT 
GGATTACCAC AGCTTAAAAA CAAACCTGCA GGAGCCAGGA CCTTCGGAGA ACGTTCCATG 
AGCAGATTTC CTTCAAAACC AAATGAAGTG CCTCACACTG AAGAGCTTTT GGATGATTCA 
ACCGTGTGGG GCGTCCGCTG CACCAGAGCC CTGGCCCCCA GTCCCAGGAG CCCTGGAGAC 
TTCACCGCTG CTGCCCGGCT CGCGTCCACG CCCTTCCACA AACCTCCGGT GGATTCAGGG 
CACCCTCTAG AGGATAAAGA AAATGTGATG GCAAGACAGT ACACCCATGT GACGTTGGAT 
TCTTGTGAAG GAAACACGGT GGAACCTTCA AAAGAGAGAA AGTGCAGTCC AGTTCAAGAG 
AAAAGCCCGG AACCAGCGTT CCCCACAGAG CTCTCTTCGG CATCCCTGCT CCGCCTGGGC 
CAGCCTTCTG CAGCCGTGCT GACCAGCGAG GCAGAGCGGG GCCTCCAGGC TTGTAAACTC 
ACAGACCCTG ACTTCGTCAC TGCAGAGGGC CCATCAGACA CCAGCGTGGG GCTCTGGGCG 
GGACAGATGC AGACCGGTCC ACTCAGGAAT ATCGATGTTC CAAACTTCAC TATTGAGGAT 
CCTTGGGATG AGGAGTTGAT TCTCAAACTT CTGTCTGGGC TTCCGAAACC AGTGACTTCC 
TATCCAAACA CTTATGAGTG GCATTGTAGA CTTCCAGCCA TCAGGCCCAA GACTGAGTTT 
CAATTGGGTT CTTTGCTGGT CTATGTTGAT CACCTTCTGG GAGAAGGGGC CTTCGCCCAA 
GTCTATGAAG TTACCCACGG GGATGTGAAC GACAGCAAAA ATAAACAGAA ATTCATTTTG 
AAGGTCCAGA AGCCCGCCAG CCCCTGGGAG TTCTACGTGG GGACCCAGCT GATGGAGAGA 
CTGAAGCCAA GTGCGCGGCA CATGTTCATC AAATTCTACT CTGCCCACCT GTTCCAGAAC 
GGCAGCGTGT TGGTCGGCGA GCTCTACAGC TACGGGACGC TCTTAAACAC CGTCAACCTC 
TATAAAAATA CCCCCGAGAA GGCGATGCCA CAAGCCCTAG TCCTCTCTTT CGCTATCAGA 
ATGCTGTCCA TGATCGAACA GCTCCACGGC TGCGAGATCA TCCACGGAGA CATCAAGCCA 
GACAACTTCA TTCTCGGAAA CAGATTTTTG GAACAAGACG GTGAAGATGA TGATTTATCT 
GCTGGCTTGG CACTGATTGA CCTGGGTCAG AGCATAGACA TGAAGCTCTT TCCAAAAGGA 
ACTGCATTTA CGGGAAAGTG TGAAACATCT GGGTTTCAGT GTATTGAGAT GATCTGTGAC 
AAGCCCTGGA ACTACCAGAT TGATTACTTT GGAGTTGCCG CAACAGTGTA CTGCATGCTT 
TTTGGCACTT ACATGAAAGT GAGACATGAA GGGGGCGTCT GGAGGCCTGA AGGTCTTTTT 
AGAAGGCTTC CTCATTTGGA TATGTGGAGT GAATTTTTCC ACATAATGTT GAATATACCA 
GACTGTCATC ATTTTCCATC TTTGGATTTA TTAAGGCAAA AGCTGAAGAA AGTACTTCAA 
GAACACTATG CAAACAAGAT TAAGACCCTA CGTAACAGGC TAATTGTGCT GCTCTTAGAA 
TACAAGCGTT CGCGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015094296.1
CDS	38..3298
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMQWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLKFAECNSDLHQFFEFLYNHGIGTQSAPLYVAWAGHLEGRGQWQHASAVFRRGIQNQAEPGELLQQQYRLFQTRLTETHVPTQARTSESLHDAQVLNQMVTTQSNPGNNSACISKNQDSELSGVISSACDKESNKEQRVIVISKSGYSAHSSLAAAADVQQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMRRKEANAFEEELLKQKMDELHKKLYQVVETSRENPPSSQERSEVNRARLGPHAGSQQEVRAPSPPAIDQQASKSAGEEPQEAPPVVPLAANAVTAALVSPAVSQSVAPPVSVSLTAQPMTDSLFTAAGKDARCVNKNNHEFKPQSAAELKEGCETHKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWPSLDQNEDAFEAQFQKNASSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQLKNKPAGARTFGERSMSRFPSKPNEVPHTEELLDDSTVWGVRCTRALAPSPRSPGDFTAAARLASTPFHKPPVDSGHPLEDKENVMARQYTHVTLDSCEGNTVEPSKERKCSPVQEKSPEPAFPTELSSASLLRLGQPSAAVLTSEAERGLQACKLTDPDFVTAEGPSDTSVGLWAGQMQTGPLRNIDVPNFTIEDPWDEELILKLLSGLPKPVTSYPNTYEWHCRLPAIRPKTEFQLGSLLVYVDHLLGEGAFAQVYEVTHGDVNDSKNKQKFILKVQKPASPWEFYVGTQLMERLKPSARHMFIKFYSAHLFQNGSVLVGELYSYGTLLNTVNLYKNTPEKAMPQALVLSFAIRMLSMIEQLHGCEIIHGDIKPDNFILGNRFLEQDGEDDDLSAGLALIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMICDKPWNYQIDYFGVAATVYCMLFGTYMKVRHEGGVWRPEGLFRRLPHLDMWSEFFHIMLNIPDCHHFPSLDLLRQKLKKVLQEHYANKIKTLRNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_015238810.1
Organism	Vicugna pacos(alpaca)
Definition	Vicugna pacos BUB1, mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015238810.1

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT CTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGATCCAC TTGGTGAATG GGAAAGTTAC ATGCAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ATTTGACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATACCACA ATGACCCCAG GTTCATCAAT TATTGTTTAA AATTTGCCGA GTGCAACAGT 
GACCTGCATC AGTTTTTTGA GTTTCTGTAC AACCACGGCA TCGGGACGCA GTCGGCTCCC 
CTGTACGTAG CCTGGGCTGG GCACCTGGAA GGCCGGGGGC AGTGGCAGCA CGCCAGCGCT 
GTTTTCCGGA GAGGGATTCA AAACCAGGCC GAACCCGGGG AGCTGCTGCA ACAGCAATAC 
AGGTTGTTTC AGACCCGTCT CACCGAAACC CATGTGCCAA CTCAAGCGAG AACCTCAGAA 
TCTCTGCATG ATGCTCAGGT TTTAAACCAA ATGGTAACAA CACAATCAAA TCCAGGAAAT 
AACTCAGCCT GCATTTCTAA GAATCAGGAT TCAGAACTTT CTGGAGTAAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAA GGAGCAAAGG GTGATCGTGA TTTCTAAGTC AGGATACTCC 
GCACACTCAT CTTTGGCAGC CGCAGCTGAC GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GTGGAGAATC AGAATTTTCC TTTGAAGAAC TGAGAGCACA GAAATACAAT 
CAGCGGAGAA AGCACGAACA GTGGGTAAAC GAAGACAGGC ATTATATGAG AAGGAAAGAA 
GCAAATGCTT TTGAAGAGGA ACTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
TACCAAGTGG TGGAGACGTC GCGGGAGAAC CCACCCTCTT CCCAGGAGAG ATCTGAGGTT 
AATCGCGCCC GTCTGGGGCC ACATGCAGGC TCCCAGCAGG AAGTGAGAGC ACCGAGTCCT 
CCAGCCATCG ATCAGCAGGC CTCAAAGAGC GCGGGAGAGG AACCGCAAGA GGCGCCTCCT 
GTTGTCCCTC TGGCGGCAAA TGCTGTCACT GCTGCTTTGG TGTCCCCAGC CGTCAGCCAG 
AGTGTAGCTC CCCCGGTTTC GGTTTCTCTG ACAGCCCAGC CCATGACAGA CTCCCTGTTT 
ACAGCGGCCG GCAAAGATGC CAGGTGTGTG AATAAAAATA ATCATGAATT CAAGCCTCAG 
AGTGCAGCAG AGCTGAAAGA AGGATGTGAA ACACATAAGG TTGTTAACAC AAGTTCTTTT 
CACACAACTC CAAACACATC ACTGGGACTG GTTCAGGCCA CACCATCCAA AGTGCAGCCG 
TCACCCACTG TGCACACCAA AGAAGCACTG GGCTTCATCA TGAATATGTT TCAGGCTCCT 
ACTCTTCCTG ACATTTCTGA TGACAAAGAT GAATGGCCAT CTCTAGATCA AAATGAAGAC 
GCATTTGAAG CCCAGTTTCA AAAAAATGCA AGTTCTTCCG GGGCTTGGGG AGTCAACAAG 
ATTATCTCTT CTTTGTCATC TGCTTTTCCT GTATTTGAAG ATGGAAACAA AGAAAATTAT 
GGATTACCAC AGCTTAAAAA CAAACCTGCA GGAGCCAGGA CCTTCGGAGA ACGTTCCATG 
AGCAGATTTC CTTCAAAACC AAATGAAGTG CCTCACACTG AAGAGCTTTT GGATGATTCA 
ACCGTGTGGG GCGTCCGCTG CACCAGAGCC CTGGCCCCCA GTCCCAGGAG CCCTGGAGAC 
TTCACCGCTG CTGCCCGGCT CGCGTCCACG CCCTTCCACA AACCTCCGGT GGATTCAGGG 
CACCCTCTAG AGGATAAAGA AAATGTGATG GCAAGACAGT ACACCCATGT GACGTTGGAT 
TCTTGTGAAG GAAACACGGT GGAACCTTCA AAAGAGAGAA AGTGCAGTCC AGTTCAAGAG 
AAAAGCCCGG AACCAGCGTT CCCCACAGAG CTCTCTTCGG CATCCCTGCT CCGCCTGGGC 
CAGCCTTCTG CAGCCGTGCT GACCAGCGAG GCAGAGCGGG GCCTCCAGGC TTGTAAACTC 
ACAGACCCTG ACTTCGTCAC TGCAGAGGGC CCATCAGACA CCAGCGTGGG GCTCTGGGCG 
GGACAGATGC AGACCGGTCC ACTCAGGAAT ATCGATGTTC CAAACTTCAC TATTGAGGAT 
CCTTGGGATG AGGAGTTGAT TCTCAAACTT CTGTCTGGGC TTCCGAAACC AGTGACTTCC 
TATCCAAACA CTTATGAGTG GCATTGTAGA CTTCCAGCCA TCAGGCCCAA GACTGAGTTT 
CAATTGGGTT CTTTGCTGGT CTATGTTGAT CACCTTCTGG GAGAAGGGGC CTTCGCCCAA 
GTCTATGAAG TTACCCACGG GGATGTGAAC GACAGCAAAA ATAAACAGAA ATTCATTTTG 
AAGGTCCAGA AGCCCGCCAG CCCCTGGGAG TTCTACGTGG GGACCCAGCT GATGGAGAGA 
CTGAAGCCAA GTGCGCGGCA CATGTTCATC AAATTCTACT CTGCCCACCT GTTCCAGAAC 
GGCAGCGTGT TGGTCGGCGA GCTCTACAGC TACGGGACGC TCTTAAACAC CGTCAACCTC 
TATAAAAATA CCCCCGAGAA GGCGATGCCA CAAGCCCTAG TCCTCTCTTT CGCTATCAGA 
ATGCTGTCCA TGATCGAACA GCTCCACGGC TGCGAGATCA TCCACGGAGA CATCAAGCCA 
GACAACTTCA TTCTCGGAAA CAGATTTTTG GAACAAGACG GTGAAGATGA TGATTTATCT 
GCTGGCTTGG CACTGATTGA CCTGGGTCAG AGCATAGACA TGAAGCTCTT TCCAAAAGGA 
ACTGCATTTA CGGGAAAGTG TGAAACATCT GGGTTTCAGT GTATTGAGAT GATCTGTGAC 
AAGCCCTGGA ACTACCAGAT TGATTACTTT GGAGTTGCCG CAACAGTGTA CTGCATGCTT 
TTTGGCACTT ACATGAAAGT GAGACATGAA GGGGGCGTCT GGAGGCCTGA AGGTCTTTTT 
AGAAGGCTTC CTCATTTGGA TATGTGGAGT GAATTTTTCC ACATAATGTT GAATATACCA 
GACTGTCATC ATTTTCCATC TTTGGATTTA TTAAGGCAAA AGCTGAAGAA AGTACTTCAA 
GAACACTATG CAAACAAGAT TAAGACCCTA CGTAACAGGC TAATTGTGCT GCTCTTAGAA 
TACAAGCGTT CGCGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_015238810.2 ) cDNA ORF clone, Vicugna pacos(alpaca) -> XP_015094296.1 Vicugna pacos BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Price & Availability↑

CloneID	OVi39394
Clone ID Related Accession (Same CDS sequence)	XM_015238810.1 , XM_015238810.2
Accession Version	XM_015238810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-21
Organism	Vicugna pacos(alpaca)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021964212.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2019 this sequence version replaced XM_015238810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Vicugna pacos Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT CTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGATCCAC TTGGTGAATG GGAAAGTTAC ATGCAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ATTTGACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATACCACA ATGACCCCAG GTTCATCAAT TATTGTTTAA AATTTGCCGA GTGCAACAGT 
GACCTGCATC AGTTTTTTGA GTTTCTGTAC AACCACGGCA TCGGGACGCA GTCGGCTCCC 
CTGTACGTAG CCTGGGCTGG GCACCTGGAA GGCCGGGGGC AGTGGCAGCA CGCCAGCGCT 
GTTTTCCGGA GAGGGATTCA AAACCAGGCC GAACCCGGGG AGCTGCTGCA ACAGCAATAC 
AGGTTGTTTC AGACCCGTCT CACCGAAACC CATGTGCCAA CTCAAGCGAG AACCTCAGAA 
TCTCTGCATG ATGCTCAGGT TTTAAACCAA ATGGTAACAA CACAATCAAA TCCAGGAAAT 
AACTCAGCCT GCATTTCTAA GAATCAGGAT TCAGAACTTT CTGGAGTAAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAA GGAGCAAAGG GTGATCGTGA TTTCTAAGTC AGGATACTCC 
GCACACTCAT CTTTGGCAGC CGCAGCTGAC GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GTGGAGAATC AGAATTTTCC TTTGAAGAAC TGAGAGCACA GAAATACAAT 
CAGCGGAGAA AGCACGAACA GTGGGTAAAC GAAGACAGGC ATTATATGAG AAGGAAAGAA 
GCAAATGCTT TTGAAGAGGA ACTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
TACCAAGTGG TGGAGACGTC GCGGGAGAAC CCACCCTCTT CCCAGGAGAG ATCTGAGGTT 
AATCGCGCCC GTCTGGGGCC ACATGCAGGC TCCCAGCAGG AAGTGAGAGC ACCGAGTCCT 
CCAGCCATCG ATCAGCAGGC CTCAAAGAGC GCGGGAGAGG AACCGCAAGA GGCGCCTCCT 
GTTGTCCCTC TGGCGGCAAA TGCTGTCACT GCTGCTTTGG TGTCCCCAGC CGTCAGCCAG 
AGTGTAGCTC CCCCGGTTTC GGTTTCTCTG ACAGCCCAGC CCATGACAGA CTCCCTGTTT 
ACAGCGGCCG GCAAAGATGC CAGGTGTGTG AATAAAAATA ATCATGAATT CAAGCCTCAG 
AGTGCAGCAG AGCTGAAAGA AGGATGTGAA ACACATAAGG TTGTTAACAC AAGTTCTTTT 
CACACAACTC CAAACACATC ACTGGGACTG GTTCAGGCCA CACCATCCAA AGTGCAGCCG 
TCACCCACTG TGCACACCAA AGAAGCACTG GGCTTCATCA TGAATATGTT TCAGGCTCCT 
ACTCTTCCTG ACATTTCTGA TGACAAAGAT GAATGGCCAT CTCTAGATCA AAATGAAGAC 
GCATTTGAAG CCCAGTTTCA AAAAAATGCA AGTTCTTCCG GGGCTTGGGG AGTCAACAAG 
ATTATCTCTT CTTTGTCATC TGCTTTTCCT GTATTTGAAG ATGGAAACAA AGAAAATTAT 
GGATTACCAC AGCTTAAAAA CAAACCTGCA GGAGCCAGGA CCTTCGGAGA ACGTTCCATG 
AGCAGATTTC CTTCAAAACC AAATGAAGTG CCTCACACTG AAGAGCTTTT GGATGATTCA 
ACCGTGTGGG GCGTCCGCTG CACCAGAGCC CTGGCCCCCA GTCCCAGGAG CCCTGGAGAC 
TTCACCGCTG CTGCCCGGCT CGCGTCCACG CCCTTCCACA AACCTCCGGT GGATTCAGGG 
CACCCTCTAG AGGATAAAGA AAATGTGATG GCAAGACAGT ACACCCATGT GACGTTGGAT 
TCTTGTGAAG GAAACACGGT GGAACCTTCA AAAGAGAGAA AGTGCAGTCC AGTTCAAGAG 
AAAAGCCCGG AACCAGCGTT CCCCACAGAG CTCTCTTCGG CATCCCTGCT CCGCCTGGGC 
CAGCCTTCTG CAGCCGTGCT GACCAGCGAG GCAGAGCGGG GCCTCCAGGC TTGTAAACTC 
ACAGACCCTG ACTTCGTCAC TGCAGAGGGC CCATCAGACA CCAGCGTGGG GCTCTGGGCG 
GGACAGATGC AGACCGGTCC ACTCAGGAAT ATCGATGTTC CAAACTTCAC TATTGAGGAT 
CCTTGGGATG AGGAGTTGAT TCTCAAACTT CTGTCTGGGC TTCCGAAACC AGTGACTTCC 
TATCCAAACA CTTATGAGTG GCATTGTAGA CTTCCAGCCA TCAGGCCCAA GACTGAGTTT 
CAATTGGGTT CTTTGCTGGT CTATGTTGAT CACCTTCTGG GAGAAGGGGC CTTCGCCCAA 
GTCTATGAAG TTACCCACGG GGATGTGAAC GACAGCAAAA ATAAACAGAA ATTCATTTTG 
AAGGTCCAGA AGCCCGCCAG CCCCTGGGAG TTCTACGTGG GGACCCAGCT GATGGAGAGA 
CTGAAGCCAA GTGCGCGGCA CATGTTCATC AAATTCTACT CTGCCCACCT GTTCCAGAAC 
GGCAGCGTGT TGGTCGGCGA GCTCTACAGC TACGGGACGC TCTTAAACAC CGTCAACCTC 
TATAAAAATA CCCCCGAGAA GGCGATGCCA CAAGCCCTAG TCCTCTCTTT CGCTATCAGA 
ATGCTGTCCA TGATCGAACA GCTCCACGGC TGCGAGATCA TCCACGGAGA CATCAAGCCA 
GACAACTTCA TTCTCGGAAA CAGATTTTTG GAACAAGACG GTGAAGATGA TGATTTATCT 
GCTGGCTTGG CACTGATTGA CCTGGGTCAG AGCATAGACA TGAAGCTCTT TCCAAAAGGA 
ACTGCATTTA CGGGAAAGTG TGAAACATCT GGGTTTCAGT GTATTGAGAT GATCTGTGAC 
AAGCCCTGGA ACTACCAGAT TGATTACTTT GGAGTTGCCG CAACAGTGTA CTGCATGCTT 
TTTGGCACTT ACATGAAAGT GAGACATGAA GGGGGCGTCT GGAGGCCTGA AGGTCTTTTT 
AGAAGGCTTC CTCATTTGGA TATGTGGAGT GAATTTTTCC ACATAATGTT GAATATACCA 
GACTGTCATC ATTTTCCATC TTTGGATTTA TTAAGGCAAA AGCTGAAGAA AGTACTTCAA 
GAACACTATG CAAACAAGAT TAAGACCCTA CGTAACAGGC TAATTGTGCT GCTCTTAGAA 
TACAAGCGTT CGCGAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015094296.1
CDS	109..3369
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMQWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLKFAECNSDLHQFFEFLYNHGIGTQSAPLYVAWAGHLEGRGQWQHASAVFRRGIQNQAEPGELLQQQYRLFQTRLTETHVPTQARTSESLHDAQVLNQMVTTQSNPGNNSACISKNQDSELSGVISSACDKESNKEQRVIVISKSGYSAHSSLAAAADVQQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMRRKEANAFEEELLKQKMDELHKKLYQVVETSRENPPSSQERSEVNRARLGPHAGSQQEVRAPSPPAIDQQASKSAGEEPQEAPPVVPLAANAVTAALVSPAVSQSVAPPVSVSLTAQPMTDSLFTAAGKDARCVNKNNHEFKPQSAAELKEGCETHKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWPSLDQNEDAFEAQFQKNASSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQLKNKPAGARTFGERSMSRFPSKPNEVPHTEELLDDSTVWGVRCTRALAPSPRSPGDFTAAARLASTPFHKPPVDSGHPLEDKENVMARQYTHVTLDSCEGNTVEPSKERKCSPVQEKSPEPAFPTELSSASLLRLGQPSAAVLTSEAERGLQACKLTDPDFVTAEGPSDTSVGLWAGQMQTGPLRNIDVPNFTIEDPWDEELILKLLSGLPKPVTSYPNTYEWHCRLPAIRPKTEFQLGSLLVYVDHLLGEGAFAQVYEVTHGDVNDSKNKQKFILKVQKPASPWEFYVGTQLMERLKPSARHMFIKFYSAHLFQNGSVLVGELYSYGTLLNTVNLYKNTPEKAMPQALVLSFAIRMLSMIEQLHGCEIIHGDIKPDNFILGNRFLEQDGEDDDLSAGLALIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMICDKPWNYQIDYFGVAATVYCMLFGTYMKVRHEGGVWRPEGLFRRLPHLDMWSEFFHIMLNIPDCHHFPSLDLLRQKLKKVLQEHYANKIKTLRNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_015238810.2
Organism	Vicugna pacos(alpaca)
Definition	Vicugna pacos BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015238810.2

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT CTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGATCCAC TTGGTGAATG GGAAAGTTAC ATGCAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ATTTGACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATACCACA ATGACCCCAG GTTCATCAAT TATTGTTTAA AATTTGCCGA GTGCAACAGT 
GACCTGCATC AGTTTTTTGA GTTTCTGTAC AACCACGGCA TCGGGACGCA GTCGGCTCCC 
CTGTACGTAG CCTGGGCTGG GCACCTGGAA GGCCGGGGGC AGTGGCAGCA CGCCAGCGCT 
GTTTTCCGGA GAGGGATTCA AAACCAGGCC GAACCCGGGG AGCTGCTGCA ACAGCAATAC 
AGGTTGTTTC AGACCCGTCT CACCGAAACC CATGTGCCAA CTCAAGCGAG AACCTCAGAA 
TCTCTGCATG ATGCTCAGGT TTTAAACCAA ATGGTAACAA CACAATCAAA TCCAGGAAAT 
AACTCAGCCT GCATTTCTAA GAATCAGGAT TCAGAACTTT CTGGAGTAAT ATCTTCAGCT 
TGTGATAAAG AGTCAAATAA GGAGCAAAGG GTGATCGTGA TTTCTAAGTC AGGATACTCC 
GCACACTCAT CTTTGGCAGC CGCAGCTGAC GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GTGGAGAATC AGAATTTTCC TTTGAAGAAC TGAGAGCACA GAAATACAAT 
CAGCGGAGAA AGCACGAACA GTGGGTAAAC GAAGACAGGC ATTATATGAG AAGGAAAGAA 
GCAAATGCTT TTGAAGAGGA ACTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 
TACCAAGTGG TGGAGACGTC GCGGGAGAAC CCACCCTCTT CCCAGGAGAG ATCTGAGGTT 
AATCGCGCCC GTCTGGGGCC ACATGCAGGC TCCCAGCAGG AAGTGAGAGC ACCGAGTCCT 
CCAGCCATCG ATCAGCAGGC CTCAAAGAGC GCGGGAGAGG AACCGCAAGA GGCGCCTCCT 
GTTGTCCCTC TGGCGGCAAA TGCTGTCACT GCTGCTTTGG TGTCCCCAGC CGTCAGCCAG 
AGTGTAGCTC CCCCGGTTTC GGTTTCTCTG ACAGCCCAGC CCATGACAGA CTCCCTGTTT 
ACAGCGGCCG GCAAAGATGC CAGGTGTGTG AATAAAAATA ATCATGAATT CAAGCCTCAG 
AGTGCAGCAG AGCTGAAAGA AGGATGTGAA ACACATAAGG TTGTTAACAC AAGTTCTTTT 
CACACAACTC CAAACACATC ACTGGGACTG GTTCAGGCCA CACCATCCAA AGTGCAGCCG 
TCACCCACTG TGCACACCAA AGAAGCACTG GGCTTCATCA TGAATATGTT TCAGGCTCCT 
ACTCTTCCTG ACATTTCTGA TGACAAAGAT GAATGGCCAT CTCTAGATCA AAATGAAGAC 
GCATTTGAAG CCCAGTTTCA AAAAAATGCA AGTTCTTCCG GGGCTTGGGG AGTCAACAAG 
ATTATCTCTT CTTTGTCATC TGCTTTTCCT GTATTTGAAG ATGGAAACAA AGAAAATTAT 
GGATTACCAC AGCTTAAAAA CAAACCTGCA GGAGCCAGGA CCTTCGGAGA ACGTTCCATG 
AGCAGATTTC CTTCAAAACC AAATGAAGTG CCTCACACTG AAGAGCTTTT GGATGATTCA 
ACCGTGTGGG GCGTCCGCTG CACCAGAGCC CTGGCCCCCA GTCCCAGGAG CCCTGGAGAC 
TTCACCGCTG CTGCCCGGCT CGCGTCCACG CCCTTCCACA AACCTCCGGT GGATTCAGGG 
CACCCTCTAG AGGATAAAGA AAATGTGATG GCAAGACAGT ACACCCATGT GACGTTGGAT 
TCTTGTGAAG GAAACACGGT GGAACCTTCA AAAGAGAGAA AGTGCAGTCC AGTTCAAGAG 
AAAAGCCCGG AACCAGCGTT CCCCACAGAG CTCTCTTCGG CATCCCTGCT CCGCCTGGGC 
CAGCCTTCTG CAGCCGTGCT GACCAGCGAG GCAGAGCGGG GCCTCCAGGC TTGTAAACTC 
ACAGACCCTG ACTTCGTCAC TGCAGAGGGC CCATCAGACA CCAGCGTGGG GCTCTGGGCG 
GGACAGATGC AGACCGGTCC ACTCAGGAAT ATCGATGTTC CAAACTTCAC TATTGAGGAT 
CCTTGGGATG AGGAGTTGAT TCTCAAACTT CTGTCTGGGC TTCCGAAACC AGTGACTTCC 
TATCCAAACA CTTATGAGTG GCATTGTAGA CTTCCAGCCA TCAGGCCCAA GACTGAGTTT 
CAATTGGGTT CTTTGCTGGT CTATGTTGAT CACCTTCTGG GAGAAGGGGC CTTCGCCCAA 
GTCTATGAAG TTACCCACGG GGATGTGAAC GACAGCAAAA ATAAACAGAA ATTCATTTTG 
AAGGTCCAGA AGCCCGCCAG CCCCTGGGAG TTCTACGTGG GGACCCAGCT GATGGAGAGA 
CTGAAGCCAA GTGCGCGGCA CATGTTCATC AAATTCTACT CTGCCCACCT GTTCCAGAAC 
GGCAGCGTGT TGGTCGGCGA GCTCTACAGC TACGGGACGC TCTTAAACAC CGTCAACCTC 
TATAAAAATA CCCCCGAGAA GGCGATGCCA CAAGCCCTAG TCCTCTCTTT CGCTATCAGA 
ATGCTGTCCA TGATCGAACA GCTCCACGGC TGCGAGATCA TCCACGGAGA CATCAAGCCA 
GACAACTTCA TTCTCGGAAA CAGATTTTTG GAACAAGACG GTGAAGATGA TGATTTATCT 
GCTGGCTTGG CACTGATTGA CCTGGGTCAG AGCATAGACA TGAAGCTCTT TCCAAAAGGA 
ACTGCATTTA CGGGAAAGTG TGAAACATCT GGGTTTCAGT GTATTGAGAT GATCTGTGAC 
AAGCCCTGGA ACTACCAGAT TGATTACTTT GGAGTTGCCG CAACAGTGTA CTGCATGCTT 
TTTGGCACTT ACATGAAAGT GAGACATGAA GGGGGCGTCT GGAGGCCTGA AGGTCTTTTT 
AGAAGGCTTC CTCATTTGGA TATGTGGAGT GAATTTTTCC ACATAATGTT GAATATACCA 
GACTGTCATC ATTTTCCATC TTTGGATTTA TTAAGGCAAA AGCTGAAGAA AGTACTTCAA 
GAACACTATG CAAACAAGAT TAAGACCCTA CGTAACAGGC TAATTGTGCT GCTCTTAGAA 
TACAAGCGTT CGCGAAAATA A