NGLY1 cDNA ORF clone, Tinamus guttatus(white-throated tinamou)

Gene
Clones
gRNAs

Summary

Gene Symbol	NGLY1
Entrez Gene ID	104580841
Full Name	N-glycanase 1
Gene Type	protein-coding
Organism	Tinamus guttatus(white-throated tinamou)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010228390.1	XP_010226692.1	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Pathways from BioSystems
Gene Ontology

The following NGLY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGLY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi14537	XM_010228390.1 Latest version!	Tinamus guttatus N-glycanase 1 (NGLY1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NGLY1 ( XM_010228390.1 ) cDNA ORF clone, Tinamus guttatus(white-throated tinamou) -> XP_010226692.1 Tinamus guttatus N-glycanase 1 (NGLY1), mRNA.

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CloneID	OTi14537
Clone ID Related Accession (Same CDS sequence)	XM_010228390.1
Accession Version	XM_010228390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-12
Organism	Tinamus guttatus(white-throated tinamou)
Product	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010578583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tinamus guttatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGCCG CCGTGGGACT GGCTTTCTCG CCGAAGTCAT GCCCCGGGCG GCGGCTTGGA 
GGTCTCGGCG TGGCCACTTA TGAAGAAAAA TACAGATTAA TTCGGATTGG AAATCCAGCA 
TTTTCTACTA GGCTGTTGCC TGTCAGAGGA GCAGTGGAAT GTTTGTTTGA AATGGGCTTT 
CAAGAGGGAG AAACTCACCT GGTTTTCCCT AAGGAAGCAT CGATTGAACA GCTACGTAAA 
GTCAGAGATT TAATTGCTGG AGAGAGAAGT AGCAGGTTGA ATGAGTCAAG TCACCTCCAC 
AGATCAAGAC CTTCTGAAAC TGCAGCCAGC ACTCAGGCAG TTGCACGTCA GCCTTCAAGA 
CCTACTGACC CTGTTCTTGC CCCTGCCCAT CAGCAGCAAG AAACATCACC TGCACAGCCT 
CTTGAAAGGG CTGCAGATAT CTTGAAAAAA CTTCAAACAA ACTTTCAACA TTTTGTATTG 
ATGTATGAGA ATCCTTCTCT TCAGCAGAAA GCACTAGCTT CAGTTCCCCT CCAAGAATTG 
AAAAGGAAGG CTCAGAAAAA GCTGGCACAA GCTACAATAC TGGATAAAGG TGCGCATGTG 
AACGAAGAAG ACTTCTTGCT GTTGGAGCTT TTGAGCTGGT TTAAGAATGA CTTTTTTCAT 
TGGGTAAATG AGCTTCCTTG CAGCAGATGT GGTGGGCAGA CAGAGCCTAA AGATAATGTG 
GCAGCTAGTG AGGACGATCT AAGGTGGAAT GCCAGCCGAG TGGAAAACCA TTACTGCAAC 
CAGTGCCAGT TCTCTAATAG ATTCCCCAGG TATAATAACC CAGAGAAACT GCTGGAAACC 
AGGCGTGGAC GTTGTGGCGA GTGGGCTAAC TGTTTTACAC TGTGTTGCAG AGCTGTAGGG 
TTTGAAGCTA GATACGTGTG GGACGTTACA GATCACGTGT GGACTGAGGT ATATTCTGCA 
TCTCAGAAAA GATGGCTTCA CTGTGATCCC TGTGAAAATG TCTGTGATAA ACCTCTTCTC 
TATGAAACTG GGTGGGGAAA GAAGCTCTCC TACATAATTG CTTTCTCCAA AGATGAGGTT 
GTTGATGTCA CCTGGAGATA CAGCTGTAAG CACGAAGAAG TGCTCTCTAG AAGGACAGCA 
GTCAGTGAAG CCACACTACG AGAAACAATT AACACCCTTA ATAAAAAGAG ACAACAATCT 
TTGTCAGAAA CCAGAAGAAG AGAGCTCCTG GAAAGGACAA TCGTGGAGCT TGTTGAGTTC 
ATTTCTCCTA AAACCCCTAA ACCAGGTGAA TTTGGTGGAA GAACATCAGG TTCAATGGCT 
TGGCGAGTAG CCAGAGGCGA AATTGGCTCT GAGAAGAGAA AAGAAATAAT TTTCATTCCC 
TCTGAAAAAG AGAAGACATC TAAGCTCTTT CACCTGACCT ACAATGTGGT AGAAGATAGT 
TACACACGGA TTTCAAATAA CAATGAAAAA ATAACTGGCT GGGAAGCAGG TGTTTGGAAG 
GCAGAGTCTA TCTGGAGGAA GGTTGAAACA GATTGGAAAA TGGTTTATTT AGCCCGAAAA 
GAGGGGTCAT CCTCTGCTTC AATCAGCTGG AAGTTTGAGT GCAAGTCAGT CGGTCTAAAA 
ATAGATAACA TATCAATTAG GACAAGTAGT CAGACATTTC AAAGTGGAAG AATCAAGTGG 
AGGGCGTACT CCCCCACAGC AGAGGTTGCT CTGCTAGGAG ATAAAAGTCT TTGCTCCTAT 
TCAGATTTGT CTGGTTCAAC AGAAGTAACA TTGGAAGCAA TCCTAAATGG AGGGGATGGG 
CAAGCTGCAT GGCAACATAC CCAGCTCTTC AGGGAGAACC TAGCAGGAAG TGGAGAGAAT 
TGCTTGGAGG TAATTATAAA ATTTGCTGAC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010226692.1
CDS	1..1896
Translation	MAAAVGLAFSPKSCPGRRLGGLGVATYEEKYRLIRIGNPAFSTRLLPVRGAVECLFEMGFQEGETHLVFPKEASIEQLRKVRDLIAGERSSRLNESSHLHRSRPSETAASTQAVARQPSRPTDPVLAPAHQQQETSPAQPLERAADILKKLQTNFQHFVLMYENPSLQQKALASVPLQELKRKAQKKLAQATILDKGAHVNEEDFLLLELLSWFKNDFFHWVNELPCSRCGGQTEPKDNVAASEDDLRWNASRVENHYCNQCQFSNRFPRYNNPEKLLETRRGRCGEWANCFTLCCRAVGFEARYVWDVTDHVWTEVYSASQKRWLHCDPCENVCDKPLLYETGWGKKLSYIIAFSKDEVVDVTWRYSCKHEEVLSRRTAVSEATLRETINTLNKKRQQSLSETRRRELLERTIVELVEFISPKTPKPGEFGGRTSGSMAWRVARGEIGSEKRKEIIFIPSEKEKTSKLFHLTYNVVEDSYTRISNNNEKITGWEAGVWKAESIWRKVETDWKMVYLARKEGSSSASISWKFECKSVGLKIDNISIRTSSQTFQSGRIKWRAYSPTAEVALLGDKSLCSYSDLSGSTEVTLEAILNGGDGQAAWQHTQLFRENLAGSGENCLEVIIKFADL

Target ORF information:

RefSeq Version	XM_010228390.1
Organism	Tinamus guttatus(white-throated tinamou)
Definition	Tinamus guttatus N-glycanase 1 (NGLY1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010228390.1

ORF Insert Sequence:

ATGGCGGCCG CCGTGGGACT GGCTTTCTCG CCGAAGTCAT GCCCCGGGCG GCGGCTTGGA 
GGTCTCGGCG TGGCCACTTA TGAAGAAAAA TACAGATTAA TTCGGATTGG AAATCCAGCA 
TTTTCTACTA GGCTGTTGCC TGTCAGAGGA GCAGTGGAAT GTTTGTTTGA AATGGGCTTT 
CAAGAGGGAG AAACTCACCT GGTTTTCCCT AAGGAAGCAT CGATTGAACA GCTACGTAAA 
GTCAGAGATT TAATTGCTGG AGAGAGAAGT AGCAGGTTGA ATGAGTCAAG TCACCTCCAC 
AGATCAAGAC CTTCTGAAAC TGCAGCCAGC ACTCAGGCAG TTGCACGTCA GCCTTCAAGA 
CCTACTGACC CTGTTCTTGC CCCTGCCCAT CAGCAGCAAG AAACATCACC TGCACAGCCT 
CTTGAAAGGG CTGCAGATAT CTTGAAAAAA CTTCAAACAA ACTTTCAACA TTTTGTATTG 
ATGTATGAGA ATCCTTCTCT TCAGCAGAAA GCACTAGCTT CAGTTCCCCT CCAAGAATTG 
AAAAGGAAGG CTCAGAAAAA GCTGGCACAA GCTACAATAC TGGATAAAGG TGCGCATGTG 
AACGAAGAAG ACTTCTTGCT GTTGGAGCTT TTGAGCTGGT TTAAGAATGA CTTTTTTCAT 
TGGGTAAATG AGCTTCCTTG CAGCAGATGT GGTGGGCAGA CAGAGCCTAA AGATAATGTG 
GCAGCTAGTG AGGACGATCT AAGGTGGAAT GCCAGCCGAG TGGAAAACCA TTACTGCAAC 
CAGTGCCAGT TCTCTAATAG ATTCCCCAGG TATAATAACC CAGAGAAACT GCTGGAAACC 
AGGCGTGGAC GTTGTGGCGA GTGGGCTAAC TGTTTTACAC TGTGTTGCAG AGCTGTAGGG 
TTTGAAGCTA GATACGTGTG GGACGTTACA GATCACGTGT GGACTGAGGT ATATTCTGCA 
TCTCAGAAAA GATGGCTTCA CTGTGATCCC TGTGAAAATG TCTGTGATAA ACCTCTTCTC 
TATGAAACTG GGTGGGGAAA GAAGCTCTCC TACATAATTG CTTTCTCCAA AGATGAGGTT 
GTTGATGTCA CCTGGAGATA CAGCTGTAAG CACGAAGAAG TGCTCTCTAG AAGGACAGCA 
GTCAGTGAAG CCACACTACG AGAAACAATT AACACCCTTA ATAAAAAGAG ACAACAATCT 
TTGTCAGAAA CCAGAAGAAG AGAGCTCCTG GAAAGGACAA TCGTGGAGCT TGTTGAGTTC 
ATTTCTCCTA AAACCCCTAA ACCAGGTGAA TTTGGTGGAA GAACATCAGG TTCAATGGCT 
TGGCGAGTAG CCAGAGGCGA AATTGGCTCT GAGAAGAGAA AAGAAATAAT TTTCATTCCC 
TCTGAAAAAG AGAAGACATC TAAGCTCTTT CACCTGACCT ACAATGTGGT AGAAGATAGT 
TACACACGGA TTTCAAATAA CAATGAAAAA ATAACTGGCT GGGAAGCAGG TGTTTGGAAG 
GCAGAGTCTA TCTGGAGGAA GGTTGAAACA GATTGGAAAA TGGTTTATTT AGCCCGAAAA 
GAGGGGTCAT CCTCTGCTTC AATCAGCTGG AAGTTTGAGT GCAAGTCAGT CGGTCTAAAA 
ATAGATAACA TATCAATTAG GACAAGTAGT CAGACATTTC AAAGTGGAAG AATCAAGTGG 
AGGGCGTACT CCCCCACAGC AGAGGTTGCT CTGCTAGGAG ATAAAAGTCT TTGCTCCTAT 
TCAGATTTGT CTGGTTCAAC AGAAGTAACA TTGGAAGCAA TCCTAAATGG AGGGGATGGG 
CAAGCTGCAT GGCAACATAC CCAGCTCTTC AGGGAGAACC TAGCAGGAAG TGGAGAGAAT 
TGCTTGGAGG TAATTATAAA ATTTGCTGAC CTGTGA