CAPN2 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN2
Entrez Gene ID	106549641
Full Name	calpain 2
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014067345.1	XP_013922820.1	LOW QUALITY PROTEIN: calpain-2 catalytic subunit

Pathways from BioSystems
Gene Ontology

The following CAPN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh25313	XM_014067345.1 Latest version!	Thamnophis sirtalis calpain 2, (m/II) large subunit (CAPN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN2 ( XM_014067345.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013922820.1 Thamnophis sirtalis calpain 2, (m/II) large subunit (CAPN2), mRNA.

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CloneID	OTh25313
Clone ID Related Accession (Same CDS sequence)	XM_014067345.1
Accession Version	XM_014067345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	LOW QUALITY PROTEIN: calpain-2 catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013659039.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGCTGGGA TTGCCGCTAA ATTAGCCAAA GACAGGCAGA TCGCGCAAGG GCTGGGCTCC 
AACTCCAAAG CCGTGAAGTA CTTGAACCAG GACTTTCACA CCCTGAGGAA CGAGTGTCTG 
GAGGCGGGCA AGCTCTTTCA AGACCCGTCT TTCCCCGCCG CCGCGCCTTC TCTGGGCTAC 
GAAGAACTAG GGCCAAACTC GCACAAAACA AAAGGCATCG TCTGGAAGAG GCCGACGGAA 
TTGTGTTCCA AACCTCAGTT TATTATTGAA GGTGCCACCC GGACTGATAT CTGCCAAGGG 
GCTTTAGGAG ACTGTTGGCT TCTAGCAGCC ATTGCTTCTC TCACCTTGAA TGAAGAAATT 
TTGGCTCGGG TGGTTCCCAG TGATCAAAGT TTCCAAGACA GTTATGCAGG AATCTTCCAC 
TTCCAGTTCT GGCAGTATGG AGAGTGGACA GATGTTGTTG TTGATGATCG ATTGCCAACC 
AAAAATGGAG ACCTACTTTT TGTTCACTCA GCAGAGAAAA ATGAATTCTG GAGTGCCCTA 
CTGGAGAAGG CCTATGCCAA GGTGAATGGT TCCTATGAAG GTCTTTCAGG GGGGAGCACT 
ACTGAGGGCT TTGAAGACTT CACTGGTGGA ATTGCAGAGT GGTATGAGCT GCAGAAAGCA 
CCACCAAACT TGTTCAAAAT AATCCAGAAA GCTCTTCAGA AAGGTTCCCT TCTTGGGTGT 
TCTATTGATA TCACAAATGC TGCTGAGACT GAGGCAGTCA CAACCCAGAA GCTGGTCAAA 
GGGCATGCAT ACTCTGTCAC AGGAGCTGAA GAGGTGAATT TCCGAGGCAG TATTCAGAAA 
CTGATCCGGA TCCGAAATCC CTGGGGAGAA GTTGAATGGA CTGGAAAATG GAATGATAAA 
TGCCCAAACT GGAATGGTAT TGATCCAAAA GTGAAAGAGA GCTTGACCAG ACAGCATGAA 
GATGGAGAAT TTTGGATGTC ATTTAGTGAT TTCCTGAGGC ATTATTCTCG TCTTGAGATC 
TGTAATTTGA CTCCAGATAC TCTGGCCACT GACAAATATA AAAAATGGAG GTTGAACTTG 
ATAGATGGAA ACTGGAGAAA AGGTTCCACG GCTGGTGGAT GCCGAAATTA TCCTAATACG 
TTTTGGACGA ACCCACAATA TTTGATTAAA CTTGAGGAAG AAGATGAGGA TCCAGATGAC 
CCAGAAAGAG GTTGTACCTT CTTGGTTGGT TTGATTCAGA AACACCGTCG GAAGCAGAGG 
AAGATGGGAG AGGATATGCA CACAATTGGC TTTGCAATCT ATGAGGTGCC TGAACAGCTT 
TCTGCCCAAA AGAAGGTCCA TCTGAGCAAA AACTACTTTC TCACAAACTG AGCAAGAGAA 
CGATCCAATA CATTTATCAA TCTTCGGGAG GTGTTAAATC GATTCAAGCT TCCCCCAGGA 
GAGTATATTA TTGTGCCATC CACCTTTGAA CCCAACAAGA ATGGGGACTT CTGCCTCAGG 
GTTTTTTCTG AAAAAAAAGC TGAATCACAA GTTGTTGATG ATGAAATTGA GGCCAATATT 
GAAGAGAAAG AACTCACTGA AGATGAAATT GAACCCAATT TTAAGAAATT ATTTAAACAA 
TTAGCTGGAG AGGATGCTGA GATCTCAGCC TTTGAACTGT GTAATATTCT GAAAAAAATC 
TTAGCAAAAC GTCAAGATAT TAAATCTGAC GGCTTTAGTA TTGAAACTTG CAAAATCATG 
GTTGATCTAT TAGATACTGA TGGAAGTGGT AAACTGGGGC TGAAAGAATT CCACATACTT 
TGGACAAAGA TCCAGAAATA CCAGCAAATT TATCGGGATA TGGATGTAGA TCGTTCTGGC 
ACCATGAATT CCTATGAGAT GCGAAAAGCC TTGGAGCAAG CAGGATTTAA GTTGGATTGC 
CAATTGCATC AAGTCATTGT GGCCCGTTTT GCTGATGAGC AGCTGATCAT TGATTTTGAT 
AACTTTGTTA GGTGCTTGAT TCGACTGGAG ACTTTGTTCA AGATATTTAA GAAACTGGAT 
ACTGAGAAAA CAGGAACAAT ACAGATGAAT CTTGACACTT GGTTGTGCTT TACTACCATA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013922820.1
CDS	92..2194
Translation	MAGIAAKLAKDRQIAQGLGSNSKAVKYLNQDFHTLRNECLEAGKLFQDPSFPAAAPSLGYEELGPNSHKTKGIVWKRPTELCSKPQFIIEGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPSDQSFQDSYAGIFHFQFWQYGEWTDVVVDDRLPTKNGDLLFVHSAEKNEFWSALLEKAYAKVNGSYEGLSGGSTTEGFEDFTGGIAEWYELQKAPPNLFKIIQKALQKGSLLGCSIDITNAAETEAVTTQKLVKGHAYSVTGAEEVNFRGSIQKLIRIRNPWGEVEWTGKWNDKCPNWNGIDPKVKESLTRQHEDGEFWMSFSDFLRHYSRLEICNLTPDTLATDKYKKWRLNLIDGNWRKGSTAGGCRNYPNTFWTNPQYLIKLEEEDEDPDDPERGCTFLVGLIQKHRRKQRKMGEDMHTIGFAIYEVPEQLSAQKKVHLSKNYFLTN*ARERSNTFINLREVLNRFKLPPGEYIIVPSTFEPNKNGDFCLRVFSEKKAESQVVDDEIEANIEEKELTEDEIEPNFKKLFKQLAGEDAEISAFELCNILKKILAKRQDIKSDGFSIETCKIMVDLLDTDGSGKLGLKEFHILWTKIQKYQQIYRDMDVDRSGTMNSYEMRKALEQAGFKLDCQLHQVIVARFADEQLIIDFDNFVRCLIRLETLFKIFKKLDTEKTGTIQMNLDTWLCFTTI

Target ORF information:

RefSeq Version	XM_014067345.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis calpain 2, (m/II) large subunit (CAPN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014067345.1

ORF Insert Sequence:

ATGGCTGGGA TTGCCGCTAA ATTAGCCAAA GACAGGCAGA TCGCGCAAGG GCTGGGCTCC 
AACTCCAAAG CCGTGAAGTA CTTGAACCAG GACTTTCACA CCCTGAGGAA CGAGTGTCTG 
GAGGCGGGCA AGCTCTTTCA AGACCCGTCT TTCCCCGCCG CCGCGCCTTC TCTGGGCTAC 
GAAGAACTAG GGCCAAACTC GCACAAAACA AAAGGCATCG TCTGGAAGAG GCCGACGGAA 
TTGTGTTCCA AACCTCAGTT TATTATTGAA GGTGCCACCC GGACTGATAT CTGCCAAGGG 
GCTTTAGGAG ACTGTTGGCT TCTAGCAGCC ATTGCTTCTC TCACCTTGAA TGAAGAAATT 
TTGGCTCGGG TGGTTCCCAG TGATCAAAGT TTCCAAGACA GTTATGCAGG AATCTTCCAC 
TTCCAGTTCT GGCAGTATGG AGAGTGGACA GATGTTGTTG TTGATGATCG ATTGCCAACC 
AAAAATGGAG ACCTACTTTT TGTTCACTCA GCAGAGAAAA ATGAATTCTG GAGTGCCCTA 
CTGGAGAAGG CCTATGCCAA GGTGAATGGT TCCTATGAAG GTCTTTCAGG GGGGAGCACT 
ACTGAGGGCT TTGAAGACTT CACTGGTGGA ATTGCAGAGT GGTATGAGCT GCAGAAAGCA 
CCACCAAACT TGTTCAAAAT AATCCAGAAA GCTCTTCAGA AAGGTTCCCT TCTTGGGTGT 
TCTATTGATA TCACAAATGC TGCTGAGACT GAGGCAGTCA CAACCCAGAA GCTGGTCAAA 
GGGCATGCAT ACTCTGTCAC AGGAGCTGAA GAGGTGAATT TCCGAGGCAG TATTCAGAAA 
CTGATCCGGA TCCGAAATCC CTGGGGAGAA GTTGAATGGA CTGGAAAATG GAATGATAAA 
TGCCCAAACT GGAATGGTAT TGATCCAAAA GTGAAAGAGA GCTTGACCAG ACAGCATGAA 
GATGGAGAAT TTTGGATGTC ATTTAGTGAT TTCCTGAGGC ATTATTCTCG TCTTGAGATC 
TGTAATTTGA CTCCAGATAC TCTGGCCACT GACAAATATA AAAAATGGAG GTTGAACTTG 
ATAGATGGAA ACTGGAGAAA AGGTTCCACG GCTGGTGGAT GCCGAAATTA TCCTAATACG 
TTTTGGACGA ACCCACAATA TTTGATTAAA CTTGAGGAAG AAGATGAGGA TCCAGATGAC 
CCAGAAAGAG GTTGTACCTT CTTGGTTGGT TTGATTCAGA AACACCGTCG GAAGCAGAGG 
AAGATGGGAG AGGATATGCA CACAATTGGC TTTGCAATCT ATGAGGTGCC TGAACAGCTT 
TCTGCCCAAA AGAAGGTCCA TCTGAGCAAA AACTACTTTC TCACAAACTG AGCAAGAGAA 
CGATCCAATA CATTTATCAA TCTTCGGGAG GTGTTAAATC GATTCAAGCT TCCCCCAGGA 
GAGTATATTA TTGTGCCATC CACCTTTGAA CCCAACAAGA ATGGGGACTT CTGCCTCAGG 
GTTTTTTCTG AAAAAAAAGC TGAATCACAA GTTGTTGATG ATGAAATTGA GGCCAATATT 
GAAGAGAAAG AACTCACTGA AGATGAAATT GAACCCAATT TTAAGAAATT ATTTAAACAA 
TTAGCTGGAG AGGATGCTGA GATCTCAGCC TTTGAACTGT GTAATATTCT GAAAAAAATC 
TTAGCAAAAC GTCAAGATAT TAAATCTGAC GGCTTTAGTA TTGAAACTTG CAAAATCATG 
GTTGATCTAT TAGATACTGA TGGAAGTGGT AAACTGGGGC TGAAAGAATT CCACATACTT 
TGGACAAAGA TCCAGAAATA CCAGCAAATT TATCGGGATA TGGATGTAGA TCGTTCTGGC 
ACCATGAATT CCTATGAGAT GCGAAAAGCC TTGGAGCAAG CAGGATTTAA GTTGGATTGC 
CAATTGCATC AAGTCATTGT GGCCCGTTTT GCTGATGAGC AGCTGATCAT TGATTTTGAT 
AACTTTGTTA GGTGCTTGAT TCGACTGGAG ACTTTGTTCA AGATATTTAA GAAACTGGAT 
ACTGAGAAAA CAGGAACAAT ACAGATGAAT CTTGACACTT GGTTGTGCTT TACTACCATA 
TGA