PTCH2 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	PTCH2
Entrez Gene ID	106547853
Full Name	patched 2
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014065118.1	XP_013920593.1	protein patched homolog 2

Pathways from BioSystems
Gene Ontology

The following PTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh23630	XM_014065118.1 Latest version!	Thamnophis sirtalis patched 2 (PTCH2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTCH2 ( XM_014065118.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013920593.1 Thamnophis sirtalis patched 2 (PTCH2), mRNA.

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CloneID	OTh23630
Clone ID Related Accession (Same CDS sequence)	XM_014065118.1
Accession Version	XM_014065118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	protein patched homolog 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658739.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGAATCCTC GCAGCATGGA TGGAAGCAAC AGGGAGCAGG ATTTGTTTGT GTGTATGGAA 
GATTATGTGA CTCCTGATCT GTGGCATGAA GGGTATAGTC CTGAAACACA TTTACCATCA 
TTTGAGGGCA GTCGTGTCAG CCAAGAGCTC CGATACACAA GAGAAAAGCT TGGTGAAGAA 
TCTCTCTACA CATCCCAGAT GCTAATACAA ACTCCCAAAC AGGAAGGAGA AAACATCCTA 
ACTCCAGATG CCTTGGAAAT GCATCTTGAA GCTGCTTTGG CTGCTAGTAA AGTGCAAGTC 
TTACTATTTG GAAAATCATG GGATCTCAAC AAAATATGCT ACAAATCAGG CGTCCCAATA 
ATTGAGAATG GAATGATTGC ACGGATGATT GAAAAGCTCT TCCCCTGTGT TGTTGTGACT 
CCACTGGATT GTTTCTGGGA TGGTGCCAAA CTACAAGGAG GGTCTGCTTA TCTACCGGGC 
CGGCGCGATA TTCAGTGGAC CAACTTAGAT CCTATTCAGT TGATGGAGGA ACTGGGTCAA 
TTTGCCTCAC TGGAAGGATT CAAGGAGATG TTGGATAAGG CAGAGGTTGG CCAGGCCTAT 
ATGGAGCGGC CTTGCCTTGA TCCTTTTGAC CCTGAGTGCC CTTCTAGTGC TCCCAACAGC 
CAAAGCCAGA AGAAACCACA CATCCCTTCC GAACTCACAG GTGGCTGCCA TGGCTTCTCC 
CGGAAATACA TGCATTGGCA AGAGGAACTC ATCTTGGGGG GTACAGTAAA AGATCCTCAA 
GGAAAACTGC AAAAAGCTGA AGCCCTGCAA AGCATGTTCC TGCTGATGAG CCCAAGACAA 
CTTTATGAGC ACTACCGGGA TGACTATGAG ATACATGATA TCAGCTGGAG CGAGGAGAAG 
GCTGCCGCTG TGCTGGAGGC TTGGCAGCGG GAATTCGTTG AGGTGGCCCA GGAATCTCTG 
CCTTCAAATT ATTCTCAGAA CATTCATGCC TTCTCTACCA CCACACTCAG TGATATGATG 
AAGTCCTTTT CAGATGTGAG TGCCATCCGT GTGGCTGGTG GTTATCTGCT AATGCTAGCG 
TATGCTTGCG TCACCCTGTT GCGCTGGGAC TGCTCCAAAT CGCAGGGTGC TGTGGGCCTG 
GCTGGTGTTC TCCTAGTTGC CCTTTCAGTA GCAGCTGGAC TTGGACTTTG CTCCTTGCTT 
GGAATTTCAT TCAATGCAGC CACCACTCAG GTGCTGCCCT TCCTGGCACT CGGCATTGGC 
GTGGATGACA TGTTCTTGCT AGCACATGCC TTCACGGAGA CTAGTCAGCA TGTCCCTGTC 
AAGGAACGGA CTGGAGAATG CCTACGGCGT AGTGGCACCA GTGTGGCACT CACATCTGTC 
AATAATATGA TTGCCTTCTT CATGGCTGCC CTTGTGCCTA TCCCTGCATT GCGTGCCTTC 
TCTCTACAGG CTGCAGTAGT GGTGGTTTTT AACTTTGCCA TGGTGCTCTT CATTTTTCCT 
GCCATTCTGA GTCTGGATCT TCACAGGCGT GAAAACAAGA GGCTTGATAT TCTCTGCTGC 
TTCTACAGCC TCTGTTCTGC TCGTGTCATC CAGATCCAAC CACAAGAATA TGCTGATGTG 
AATGACAACC ACACTGCACC GACTTATCCC CATGGCCATC CAGCCCTGGC GACCAGCACA 
CACATCACTA CCACTGTGCA GGCCTTCACT CAGTGTGACT CCTCTGGGAG ACATGTAGTC 
ACTATTTTAC CACCCACACA GCATGTGTTT ACCAATCCCT CCACTCTCCT TCCTGCCAAT 
CCTCTGGGCT CGCAAATCTT CACTCCTGCC AGTTCCACTC GAGATCTTCT GGCACAGCTA 
GATGAAAGCA AAGGAGCCCA AGAATGTGTG CCCTTGCCGA TTTGTCGATG GAACCTTTCC 
AGCTTTGCTC GTGACAGATA TGCACCCATC CTGCTGCAGA GCCAAACTAA GGGAATTGTG 
GTAGCTCTAT TCCTAGCCCT GCTGGGCCTC AGCCTCTATG GTACAACCAA GGTGCATGAT 
GGACTCTATC TGACGGATAT TGTACCACGA GGTACAAAGG AGCATGCCTT CATTTCAGCT 
CAATTCAAGT ATTTCTCCTT TTACAACATG TTTGTGGTAA CGAATGGAGG CTTTGATTAC 
CCAAACTCAC AGGAAGCTCT TTACAGCCTA CACAAGACCT TTGCTGCCAT CCAGTATGTA 
GTACGTGAGG AGAACCGCCA GTTGCCAAAG ATGTGGCTGC ACTACTTCCG AGATTGGCTA 
CAAGATCTAC AGGCAGCCTT CAATCAAGAC TGGAAAGCCG GGCACATCAC CAGGGAAAAT 
TATCGGAATG GTTCGGAAGA TGGTGTTCTT GCTTATAAAC TCCTTATCCA GACGGGCAAC 
AAGAAAGAGC CTTTCAACTT AAACCAACTG ACAACTCGCC GTCTCGTCGA TGAAAATGGG 
ATCATTCCCC CTGATATCTT TTACATCTGC TTGACAGTGT GGGTCAGCAA TGACCCCCTT 
GGTTTTGCTG CCTCCCAGGC CAACTTCTAT CCGCCACCCC CGGAATGGAT CCATGATAAA 
TATGATGCTA CTGGTGAAAA TCTTCGAATC CCAGCTGCAC AGCCTCTGGA GTTTGCTCAG 
TTCCCCTTCT ACCTCAATGG CCTTCAACAT ACATCAGACT TCGTGGCAGC TATCCAGAGT 
GTGCGTGCCA TCTGTGATGA AATGGCCCAG ACACATGGAG TATTTAGTTA CCCCAGTGGT 
TATCCATTCC TCTTCTGGGA GCAGTACATT GGCCTGCGCC ACTGGCTTCT GCAAGCCATC 
AGCATTGTGC TGGCCTGCAC ATTTCTGGTG TGCACCCTGC TGCTACTCAA TCCTTGGACT 
GCCAGTATCA TTGTATTTGT TTTGGCAATG ATGACAGTTG AGCTTTTTGG CATCATGGGC 
TTGATGGGCA TCAAACTGAG TGCAATCCCT GTTGTCATCC TAATTGCTTC TGTAGGCATT 
GGCGTGGAAT TTACTGTACA TGTAGCTCTG GGGTTTCTCA CCGCCATTGG CAGCCGGAAC 
CTGCGCTCTA CTGTGGCTTT AGAGCACATG TTTGCTCCTG TAATGGATGG TGCTGTTTCC 
ACTCTGCTTG GTGTACTTAT GTTGGCAGGC TCCGAATTTG ATTTCATTCT GAGATATTTC 
TTTGCCGTGC TTACTATCCT GACTATTCTG GGACTACTTA ATGGCCTGGT ACTATTGCCT 
GTACTACTTT CTATTATTGG ACCGCCTGCC GAGGTCACAC CAGCAGATAA TGGCAGCCAC 
TTGCCGACAC CGTCACCTGT CCCACCGCCT ATCAACCATC ACAGCTTCTA CATTCGCCAA 
AATGGAAATG CTTTTGGTGA CTCCTCAGAC TCAGAATACT ATTCAGAGAC AGTTAGTGCA 
TCAGAGACTG GTGAAGATGA CCGCAGCACA TATATCATCT CCCCAGCTCA ATCACAGATC 
TTGGTCAAGG CCAGCAAAAG TCCTAATTTT CCCCAAATCA CTGTAGTAAA AAGCTACAAG 
ACAAGCCTAG AATCACCTTT ATCATTGTGC AGCCCAGAAT GGACCCAATC CAGAACTCCT 
GGTGGCTATT TGCCAGCAGC TGTCACAGAA ATCAAAAGTT GTTTGCAGCA GCAAGAACAA 
CCCAACCACA GACTAGGGTC TCAATTGGCT CAGTCTACTC CCAATCCCCT TCAGACTAAC 
CCCATTACTC ACAGCACCAA AATACCACCT TCAGGACACG GTGGCACTTT CACTACAGTT 
ACAGCTACTG CCTCGGTGAT GTTGGCCTTG CACCCACCTT TGCCTGGATC TTGCCGGGGC 
TATACACATG AGGGCTTTGA ATCTGATGAT AACTCTGAAA AACCTGGCCC CAAACCATCT 
GACAGTTTGG AACTACAGGA TCTAAAGCAG AATCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013920593.1
CDS	1..3939
Translation	MNPRSMDGSNREQDLFVCMEDYVTPDLWHEGYSPETHLPSFEGSRVSQELRYTREKLGEESLYTSQMLIQTPKQEGENILTPDALEMHLEAALAASKVQVLLFGKSWDLNKICYKSGVPIIENGMIARMIEKLFPCVVVTPLDCFWDGAKLQGGSAYLPGRRDIQWTNLDPIQLMEELGQFASLEGFKEMLDKAEVGQAYMERPCLDPFDPECPSSAPNSQSQKKPHIPSELTGGCHGFSRKYMHWQEELILGGTVKDPQGKLQKAEALQSMFLLMSPRQLYEHYRDDYEIHDISWSEEKAAAVLEAWQREFVEVAQESLPSNYSQNIHAFSTTTLSDMMKSFSDVSAIRVAGGYLLMLAYACVTLLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLLGISFNAATTQVLPFLALGIGVDDMFLLAHAFTETSQHVPVKERTGECLRRSGTSVALTSVNNMIAFFMAALVPIPALRAFSLQAAVVVVFNFAMVLFIFPAILSLDLHRRENKRLDILCCFYSLCSARVIQIQPQEYADVNDNHTAPTYPHGHPALATSTHITTTVQAFTQCDSSGRHVVTILPPTQHVFTNPSTLLPANPLGSQIFTPASSTRDLLAQLDESKGAQECVPLPICRWNLSSFARDRYAPILLQSQTKGIVVALFLALLGLSLYGTTKVHDGLYLTDIVPRGTKEHAFISAQFKYFSFYNMFVVTNGGFDYPNSQEALYSLHKTFAAIQYVVREENRQLPKMWLHYFRDWLQDLQAAFNQDWKAGHITRENYRNGSEDGVLAYKLLIQTGNKKEPFNLNQLTTRRLVDENGIIPPDIFYICLTVWVSNDPLGFAASQANFYPPPPEWIHDKYDATGENLRIPAAQPLEFAQFPFYLNGLQHTSDFVAAIQSVRAICDEMAQTHGVFSYPSGYPFLFWEQYIGLRHWLLQAISIVLACTFLVCTLLLLNPWTASIIVFVLAMMTVELFGIMGLMGIKLSAIPVVILIASVGIGVEFTVHVALGFLTAIGSRNLRSTVALEHMFAPVMDGAVSTLLGVLMLAGSEFDFILRYFFAVLTILTILGLLNGLVLLPVLLSIIGPPAEVTPADNGSHLPTPSPVPPPINHHSFYIRQNGNAFGDSSDSEYYSETVSASETGEDDRSTYIISPAQSQILVKASKSPNFPQITVVKSYKTSLESPLSLCSPEWTQSRTPGGYLPAAVTEIKSCLQQQEQPNHRLGSQLAQSTPNPLQTNPITHSTKIPPSGHGGTFTTVTATASVMLALHPPLPGSCRGYTHEGFESDDNSEKPGPKPSDSLELQDLKQNH

Target ORF information:

RefSeq Version	XM_014065118.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis patched 2 (PTCH2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014065118.1

ORF Insert Sequence:

ATGAATCCTC GCAGCATGGA TGGAAGCAAC AGGGAGCAGG ATTTGTTTGT GTGTATGGAA 
GATTATGTGA CTCCTGATCT GTGGCATGAA GGGTATAGTC CTGAAACACA TTTACCATCA 
TTTGAGGGCA GTCGTGTCAG CCAAGAGCTC CGATACACAA GAGAAAAGCT TGGTGAAGAA 
TCTCTCTACA CATCCCAGAT GCTAATACAA ACTCCCAAAC AGGAAGGAGA AAACATCCTA 
ACTCCAGATG CCTTGGAAAT GCATCTTGAA GCTGCTTTGG CTGCTAGTAA AGTGCAAGTC 
TTACTATTTG GAAAATCATG GGATCTCAAC AAAATATGCT ACAAATCAGG CGTCCCAATA 
ATTGAGAATG GAATGATTGC ACGGATGATT GAAAAGCTCT TCCCCTGTGT TGTTGTGACT 
CCACTGGATT GTTTCTGGGA TGGTGCCAAA CTACAAGGAG GGTCTGCTTA TCTACCGGGC 
CGGCGCGATA TTCAGTGGAC CAACTTAGAT CCTATTCAGT TGATGGAGGA ACTGGGTCAA 
TTTGCCTCAC TGGAAGGATT CAAGGAGATG TTGGATAAGG CAGAGGTTGG CCAGGCCTAT 
ATGGAGCGGC CTTGCCTTGA TCCTTTTGAC CCTGAGTGCC CTTCTAGTGC TCCCAACAGC 
CAAAGCCAGA AGAAACCACA CATCCCTTCC GAACTCACAG GTGGCTGCCA TGGCTTCTCC 
CGGAAATACA TGCATTGGCA AGAGGAACTC ATCTTGGGGG GTACAGTAAA AGATCCTCAA 
GGAAAACTGC AAAAAGCTGA AGCCCTGCAA AGCATGTTCC TGCTGATGAG CCCAAGACAA 
CTTTATGAGC ACTACCGGGA TGACTATGAG ATACATGATA TCAGCTGGAG CGAGGAGAAG 
GCTGCCGCTG TGCTGGAGGC TTGGCAGCGG GAATTCGTTG AGGTGGCCCA GGAATCTCTG 
CCTTCAAATT ATTCTCAGAA CATTCATGCC TTCTCTACCA CCACACTCAG TGATATGATG 
AAGTCCTTTT CAGATGTGAG TGCCATCCGT GTGGCTGGTG GTTATCTGCT AATGCTAGCG 
TATGCTTGCG TCACCCTGTT GCGCTGGGAC TGCTCCAAAT CGCAGGGTGC TGTGGGCCTG 
GCTGGTGTTC TCCTAGTTGC CCTTTCAGTA GCAGCTGGAC TTGGACTTTG CTCCTTGCTT 
GGAATTTCAT TCAATGCAGC CACCACTCAG GTGCTGCCCT TCCTGGCACT CGGCATTGGC 
GTGGATGACA TGTTCTTGCT AGCACATGCC TTCACGGAGA CTAGTCAGCA TGTCCCTGTC 
AAGGAACGGA CTGGAGAATG CCTACGGCGT AGTGGCACCA GTGTGGCACT CACATCTGTC 
AATAATATGA TTGCCTTCTT CATGGCTGCC CTTGTGCCTA TCCCTGCATT GCGTGCCTTC 
TCTCTACAGG CTGCAGTAGT GGTGGTTTTT AACTTTGCCA TGGTGCTCTT CATTTTTCCT 
GCCATTCTGA GTCTGGATCT TCACAGGCGT GAAAACAAGA GGCTTGATAT TCTCTGCTGC 
TTCTACAGCC TCTGTTCTGC TCGTGTCATC CAGATCCAAC CACAAGAATA TGCTGATGTG 
AATGACAACC ACACTGCACC GACTTATCCC CATGGCCATC CAGCCCTGGC GACCAGCACA 
CACATCACTA CCACTGTGCA GGCCTTCACT CAGTGTGACT CCTCTGGGAG ACATGTAGTC 
ACTATTTTAC CACCCACACA GCATGTGTTT ACCAATCCCT CCACTCTCCT TCCTGCCAAT 
CCTCTGGGCT CGCAAATCTT CACTCCTGCC AGTTCCACTC GAGATCTTCT GGCACAGCTA 
GATGAAAGCA AAGGAGCCCA AGAATGTGTG CCCTTGCCGA TTTGTCGATG GAACCTTTCC 
AGCTTTGCTC GTGACAGATA TGCACCCATC CTGCTGCAGA GCCAAACTAA GGGAATTGTG 
GTAGCTCTAT TCCTAGCCCT GCTGGGCCTC AGCCTCTATG GTACAACCAA GGTGCATGAT 
GGACTCTATC TGACGGATAT TGTACCACGA GGTACAAAGG AGCATGCCTT CATTTCAGCT 
CAATTCAAGT ATTTCTCCTT TTACAACATG TTTGTGGTAA CGAATGGAGG CTTTGATTAC 
CCAAACTCAC AGGAAGCTCT TTACAGCCTA CACAAGACCT TTGCTGCCAT CCAGTATGTA 
GTACGTGAGG AGAACCGCCA GTTGCCAAAG ATGTGGCTGC ACTACTTCCG AGATTGGCTA 
CAAGATCTAC AGGCAGCCTT CAATCAAGAC TGGAAAGCCG GGCACATCAC CAGGGAAAAT 
TATCGGAATG GTTCGGAAGA TGGTGTTCTT GCTTATAAAC TCCTTATCCA GACGGGCAAC 
AAGAAAGAGC CTTTCAACTT AAACCAACTG ACAACTCGCC GTCTCGTCGA TGAAAATGGG 
ATCATTCCCC CTGATATCTT TTACATCTGC TTGACAGTGT GGGTCAGCAA TGACCCCCTT 
GGTTTTGCTG CCTCCCAGGC CAACTTCTAT CCGCCACCCC CGGAATGGAT CCATGATAAA 
TATGATGCTA CTGGTGAAAA TCTTCGAATC CCAGCTGCAC AGCCTCTGGA GTTTGCTCAG 
TTCCCCTTCT ACCTCAATGG CCTTCAACAT ACATCAGACT TCGTGGCAGC TATCCAGAGT 
GTGCGTGCCA TCTGTGATGA AATGGCCCAG ACACATGGAG TATTTAGTTA CCCCAGTGGT 
TATCCATTCC TCTTCTGGGA GCAGTACATT GGCCTGCGCC ACTGGCTTCT GCAAGCCATC 
AGCATTGTGC TGGCCTGCAC ATTTCTGGTG TGCACCCTGC TGCTACTCAA TCCTTGGACT 
GCCAGTATCA TTGTATTTGT TTTGGCAATG ATGACAGTTG AGCTTTTTGG CATCATGGGC 
TTGATGGGCA TCAAACTGAG TGCAATCCCT GTTGTCATCC TAATTGCTTC TGTAGGCATT 
GGCGTGGAAT TTACTGTACA TGTAGCTCTG GGGTTTCTCA CCGCCATTGG CAGCCGGAAC 
CTGCGCTCTA CTGTGGCTTT AGAGCACATG TTTGCTCCTG TAATGGATGG TGCTGTTTCC 
ACTCTGCTTG GTGTACTTAT GTTGGCAGGC TCCGAATTTG ATTTCATTCT GAGATATTTC 
TTTGCCGTGC TTACTATCCT GACTATTCTG GGACTACTTA ATGGCCTGGT ACTATTGCCT 
GTACTACTTT CTATTATTGG ACCGCCTGCC GAGGTCACAC CAGCAGATAA TGGCAGCCAC 
TTGCCGACAC CGTCACCTGT CCCACCGCCT ATCAACCATC ACAGCTTCTA CATTCGCCAA 
AATGGAAATG CTTTTGGTGA CTCCTCAGAC TCAGAATACT ATTCAGAGAC AGTTAGTGCA 
TCAGAGACTG GTGAAGATGA CCGCAGCACA TATATCATCT CCCCAGCTCA ATCACAGATC 
TTGGTCAAGG CCAGCAAAAG TCCTAATTTT CCCCAAATCA CTGTAGTAAA AAGCTACAAG 
ACAAGCCTAG AATCACCTTT ATCATTGTGC AGCCCAGAAT GGACCCAATC CAGAACTCCT 
GGTGGCTATT TGCCAGCAGC TGTCACAGAA ATCAAAAGTT GTTTGCAGCA GCAAGAACAA 
CCCAACCACA GACTAGGGTC TCAATTGGCT CAGTCTACTC CCAATCCCCT TCAGACTAAC 
CCCATTACTC ACAGCACCAA AATACCACCT TCAGGACACG GTGGCACTTT CACTACAGTT 
ACAGCTACTG CCTCGGTGAT GTTGGCCTTG CACCCACCTT TGCCTGGATC TTGCCGGGGC 
TATACACATG AGGGCTTTGA ATCTGATGAT AACTCTGAAA AACCTGGCCC CAAACCATCT 
GACAGTTTGG AACTACAGGA TCTAAAGCAG AATCATTAG