EFEMP1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	EFEMP1
Entrez Gene ID	106543258
Full Name	EGF containing fibulin extracellular matrix protein 1
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014059226.1	XP_013914701.1	EGF-containing fibulin-like extracellular matrix protein 1

Pathways from BioSystems
Gene Ontology

The following EFEMP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFEMP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh19174	XM_014059226.1 Latest version!	Thamnophis sirtalis EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFEMP1 ( XM_014059226.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013914701.1 Thamnophis sirtalis EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), mRNA.

Price & Availability↑

CloneID	OTh19174
Clone ID Related Accession (Same CDS sequence)	XM_014059226.1
Accession Version	XM_014059226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	EGF-containing fibulin-like extracellular matrix protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658199.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCATCT TTGCTGTCTT GGCTGCTCTG CTCCTTGCTA CAGGACACTC ACAAGAAGCA 
GATGAAACTA TCACATTCAC GCAATGCACA GATGGTTATG AATGGGACCC CATCATGCAG 
CGATGCAAAG ATATTGATGA ATGTGAAATT GTGCACGAAG CATGTAAAGG TGGAATGAAA 
TGTGTTAATC ACTTTGGAGG ATACCTCTGT CTGCCCAGAA CAGCCCAGAT TATTGTCAAC 
AACGGCCAAG AACAAACAGC ATCAGACACT GGTAGCAGAA ATGGTAATCC TATCCGTCGG 
ACACCTCAGG TACCATCTCA GATACAGTGT GCAGCTGGGT ACGAGGCAAA CGATCATAAT 
GTGTGCCAAG ATATTGATGA ATGTACCACC AGTGGCCACA CCTGTAGGTC AGATCAGGTC 
TGTGTCAACT TAAGGGGATC ATTCACCTGC CAGTGCCGAA CTGGGTATCA AAAACGAGGA 
GACCAGTGCT TTGACATTGA TGAATGCACG TTCCCTTCCT TTTGCCACCA TCGGTGTGTG 
AACAGTGCTG GCTCCTACCA TTGCCATTGC AATGCTGGCT TTCAGTTAAC TCCTGACAAC 
CGCACCTGTG CCGACATAAA TGAGTGTGAG ACCGGAAACC CCTGTGCCCA ACTGTGCTAC 
AATATAGTTG GTTCTTTCTT ATGCCAGTGC AATCAAGGCT TTCAGTTAAG CCCAGATAGA 
ATCAGCTGTG ATGATGTTGA TGAATGTAGA GCTTCTAACT TCCTTTGCCA ATATCAGTGT 
GTCAATGAGC CAGGAAGATA CTCCTGCGCG TGTCCAGATG GATACCAGGT GGTCAGAGGA 
AGAAACTGTC AAGATATAAA TGAATGTGAA ACACGCAATG AATGTCGAGA AGATGAGTTG 
TGTTCTAATT ATCCTGGTGG CTATCGCTGT TACCCAAGAA ATCCTTGTCA GGAGCCTTAC 
GTGCTTGCAT CTGAAAATCG CTGTATTTGT CCAGTGTCTA ATCCTCTTTG TCGGGACCTG 
CCATATTCCA TTGTTCACAA ATATATGAGC ATCCATTCAG AGAGGACTGT GCCCTCTGAT 
ATCTTCCAAA TTCAGGCAAC CACAATTTTT CCCAATACAA TTAACACCTT CCGGATTAAA 
TCTGGAAATG ACAATGGAGA CTTCTTTCTT AGGCAAACAA GCTCAGTGAG TGCAATGCTT 
GTGTTGGTCA AGCCATTAAC AGGTCCACGA GAACATATCA TCGACCTGGA GCTGTTGACG 
GTCAACAACA TAAACTATCG TACAAGTTCA GTATTGAGGT TAACACTTAT TGTGGGACCT 
TACTCTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013914701.1
CDS	8..1339
Translation	MVIFAVLAALLLATGHSQEADETITFTQCTDGYEWDPIMQRCKDIDECEIVHEACKGGMKCVNHFGGYLCLPRTAQIIVNNGQEQTASDTGSRNGNPIRRTPQVPSQIQCAAGYEANDHNVCQDIDECTTSGHTCRSDQVCVNLRGSFTCQCRTGYQKRGDQCFDIDECTFPSFCHHRCVNSAGSYHCHCNAGFQLTPDNRTCADINECETGNPCAQLCYNIVGSFLCQCNQGFQLSPDRISCDDVDECRASNFLCQYQCVNEPGRYSCACPDGYQVVRGRNCQDINECETRNECREDELCSNYPGGYRCYPRNPCQEPYVLASENRCICPVSNPLCRDLPYSIVHKYMSIHSERTVPSDIFQIQATTIFPNTINTFRIKSGNDNGDFFLRQTSSVSAMLVLVKPLTGPREHIIDLELLTVNNINYRTSSVLRLTLIVGPYSF

Target ORF information:

RefSeq Version	XM_014059226.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014059226.1

ORF Insert Sequence:

ATGGTCATCT TTGCTGTCTT GGCTGCTCTG CTCCTTGCTA CAGGACACTC ACAAGAAGCA 
GATGAAACTA TCACATTCAC GCAATGCACA GATGGTTATG AATGGGACCC CATCATGCAG 
CGATGCAAAG ATATTGATGA ATGTGAAATT GTGCACGAAG CATGTAAAGG TGGAATGAAA 
TGTGTTAATC ACTTTGGAGG ATACCTCTGT CTGCCCAGAA CAGCCCAGAT TATTGTCAAC 
AACGGCCAAG AACAAACAGC ATCAGACACT GGTAGCAGAA ATGGTAATCC TATCCGTCGG 
ACACCTCAGG TACCATCTCA GATACAGTGT GCAGCTGGGT ACGAGGCAAA CGATCATAAT 
GTGTGCCAAG ATATTGATGA ATGTACCACC AGTGGCCACA CCTGTAGGTC AGATCAGGTC 
TGTGTCAACT TAAGGGGATC ATTCACCTGC CAGTGCCGAA CTGGGTATCA AAAACGAGGA 
GACCAGTGCT TTGACATTGA TGAATGCACG TTCCCTTCCT TTTGCCACCA TCGGTGTGTG 
AACAGTGCTG GCTCCTACCA TTGCCATTGC AATGCTGGCT TTCAGTTAAC TCCTGACAAC 
CGCACCTGTG CCGACATAAA TGAGTGTGAG ACCGGAAACC CCTGTGCCCA ACTGTGCTAC 
AATATAGTTG GTTCTTTCTT ATGCCAGTGC AATCAAGGCT TTCAGTTAAG CCCAGATAGA 
ATCAGCTGTG ATGATGTTGA TGAATGTAGA GCTTCTAACT TCCTTTGCCA ATATCAGTGT 
GTCAATGAGC CAGGAAGATA CTCCTGCGCG TGTCCAGATG GATACCAGGT GGTCAGAGGA 
AGAAACTGTC AAGATATAAA TGAATGTGAA ACACGCAATG AATGTCGAGA AGATGAGTTG 
TGTTCTAATT ATCCTGGTGG CTATCGCTGT TACCCAAGAA ATCCTTGTCA GGAGCCTTAC 
GTGCTTGCAT CTGAAAATCG CTGTATTTGT CCAGTGTCTA ATCCTCTTTG TCGGGACCTG 
CCATATTCCA TTGTTCACAA ATATATGAGC ATCCATTCAG AGAGGACTGT GCCCTCTGAT 
ATCTTCCAAA TTCAGGCAAC CACAATTTTT CCCAATACAA TTAACACCTT CCGGATTAAA 
TCTGGAAATG ACAATGGAGA CTTCTTTCTT AGGCAAACAA GCTCAGTGAG TGCAATGCTT 
GTGTTGGTCA AGCCATTAAC AGGTCCACGA GAACATATCA TCGACCTGGA GCTGTTGACG 
GTCAACAACA TAAACTATCG TACAAGTTCA GTATTGAGGT TAACACTTAT TGTGGGACCT 
TACTCTTTTT AG