PMFBP1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	PMFBP1
Entrez Gene ID	106541818
Full Name	polyamine modulated factor 1 binding protein 1
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014057357.1	XP_013912832.1	polyamine-modulated factor 1-binding protein 1

Pathways from BioSystems
Gene Ontology

The following PMFBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PMFBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh17757	XM_014057357.1 Latest version!	Thamnophis sirtalis polyamine modulated factor 1 binding protein 1 (PMFBP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PMFBP1 ( XM_014057357.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013912832.1 Thamnophis sirtalis polyamine modulated factor 1 binding protein 1 (PMFBP1), mRNA.

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CloneID	OTh17757
Clone ID Related Accession (Same CDS sequence)	XM_014057357.1
Accession Version	XM_014057357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	polyamine-modulated factor 1-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658073.1) and transcript sequence (GDKU01081543.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 75 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCAGATGG AAAGGGCTCA TCTGATGGGA CAGGAGCAAG TCAAACTGCA TGGGGATTCG 
GTGGAAGGGC AGAGCGTGTG GTCAGGGTTC CAAGACACCT GGCTGCAGAG TCCAAGCAAG 
GCTGCCCTCC CAGAGCAGAG GCGGGGCGTC CGGGTAGCCG TGCAGCAGCT GCAGCAGGAG 
AAGGGTGGCC TGAGCTGGAC GCACGGACAG CCTCAGGCCC TGGAGATGCT GTTCCAGGAG 
TGCCAGGCTC TCCAGGCCCA GCTCCGACAG TCTAAGGCAT TGACCCAGGA GCCAGAAAGG 
GAGGACGCTC TTCAAGCTGA GCGGCACCCG TGGGAGCAGT TGAGGCAAGC AGCGGCTTCT 
GTCCATATGC TGGAAAACAG CCGGGATCTC TATAAGAAGA AGTACCGAGC GGCTCTGGGC 
CGAGTTGGTG AGCTAGAGAG CCAGGTGCTT CGTCTGCAAG AAGAGGTGGT CAATCTTTGG 
AGCAGCGGCC AAGCAGCGGG CAGCCTTGGG CAGCTGAGTG CTCGGGAGAT GGGCTGGGCG 
ATGCAGCTCA CAGGCCAGCA AGAGCAGACC ATCCGGGAGC TGCAGGGACA GTTGGCAGCC 
AGCCACAGGA AGTTGCTGCA GCAAAAGGAA GCCCTGGCCG CCCTGCGCCG AGAGTTTGCA 
TCCTACAAGT CGACTCACAG CTGCTCCAAC GCAAAGCACA GGAAACAGAG GCTCAGCCAG 
GCGATGCTCC GTCAGAAGCT CCAGCAGGCA GAAGAGGAGG GTTTGCAGCA GCAGGCGGAG 
GCCTACCAGG CCCTTGTGCA GGAACTGAAG CTGCAGCTGG CAAGGGAGGC CGAGCAGAAC 
AGCAGCACCT TGAAAGATCT GGCTCGCCTG GAACTGGCTG TGCAGAGCCT GTGCCAGGAG 
CCAGCGCCAG AGCAGAGTCG GCAGCTGCAG CACCACGCCT GCCCAGCGCA GGCCCTGCGG 
GAACAAAGCC ACCCTGTGCT GGGTCCACAG AAGGAATGGG AACCAGACAA GCTCCTTCAA 
AGGGCTAATT GCAGCCTCTT CTGGGACCAG GCCCATCCCT TGAATGCTGC CTCACGGGAG 
GCCTCTTGGC CTGGTGGGGC TCCAGTGCTG CAGCCGAGGA GGCCCGAGTG CCCGGAACAG 
CCCAGGAGGG CGGCACTGGA GGTGGCCCAG GACCAGGCGG TGGAGAGGGA CCTGGAGCTT 
CAGCGCCTTG GGGATGTTGG CTGCCCGCTG GAGGTTCTCC TTGGAAGAGG CTGCCTAGCT 
GGAGCAGAGC TGGAGCAGGA AAGCTTCTTG GGAGGAGCCT CTGGCAGAGA ACGGCTGAAG 
GCGCTGAGGG AGAAGCTGAG CAGGTCCAAG CAGCCGCCTT TGCAGGCAGC ATCCATGCAG 
GAGTTGGAGA TGCTGCGGCA GCAGTTACAG GAGACCGAGA GGCGAGGGCG GGCCCTGCAG 
GCCTCCGTGG CGGAGCAGAC GGCAGAGCGC CGTTCTCTGC GAGAGCAGCT GACCAAGCAG 
ACGGCTGTTC TGTGGCTGCT GCAGGGACAC TTCAGGCAGG CCCGGCTGCA GCTGAAGCAC 
CAGGCAACCG CTATAGAATC CTTACGAGTC TCCCTGGAGG CATCCCACGC CGGCCATCGC 
TGGCTGCACC AGGAGAGCGA ACTGGTGGTG GCCGATCTTT GCCAGTGGGT GAAGGAGCAA 
AAGCAGATGA ATGAGGAGCT GGGCCGCAAG CTCCGAACGC AGATCAAGCA AATTGCCCAG 
CTGACCGGCG AGAGAGACCT TCTGCATCAA CTATTAGAGA GGCTGCAAGA GGACAACCGG 
CGGCTGAAGA ACGAAGTCAA CGAGAAGCGC GTTGTATGTG AGCGCATCAA GGCTCTCCAC 
AACAATGATT TGGAGCCACG GGCTGCACTG CAACAACTCT GGCACATACT CCTGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013912832.1
CDS	124..2043
Translation	MQMERAHLMGQEQVKLHGDSVEGQSVWSGFQDTWLQSPSKAALPEQRRGVRVAVQQLQQEKGGLSWTHGQPQALEMLFQECQALQAQLRQSKALTQEPEREDALQAERHPWEQLRQAAASVHMLENSRDLYKKKYRAALGRVGELESQVLRLQEEVVNLWSSGQAAGSLGQLSAREMGWAMQLTGQQEQTIRELQGQLAASHRKLLQQKEALAALRREFASYKSTHSCSNAKHRKQRLSQAMLRQKLQQAEEEGLQQQAEAYQALVQELKLQLAREAEQNSSTLKDLARLELAVQSLCQEPAPEQSRQLQHHACPAQALREQSHPVLGPQKEWEPDKLLQRANCSLFWDQAHPLNAASREASWPGGAPVLQPRRPECPEQPRRAALEVAQDQAVERDLELQRLGDVGCPLEVLLGRGCLAGAELEQESFLGGASGRERLKALREKLSRSKQPPLQAASMQELEMLRQQLQETERRGRALQASVAEQTAERRSLREQLTKQTAVLWLLQGHFRQARLQLKHQATAIESLRVSLEASHAGHRWLHQESELVVADLCQWVKEQKQMNEELGRKLRTQIKQIAQLTGERDLLHQLLERLQEDNRRLKNEVNEKRVVCERIKALHNNDLEPRAALQQLWHILLL

Target ORF information:

RefSeq Version	XM_014057357.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis polyamine modulated factor 1 binding protein 1 (PMFBP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014057357.1

ORF Insert Sequence:

ATGCAGATGG AAAGGGCTCA TCTGATGGGA CAGGAGCAAG TCAAACTGCA TGGGGATTCG 
GTGGAAGGGC AGAGCGTGTG GTCAGGGTTC CAAGACACCT GGCTGCAGAG TCCAAGCAAG 
GCTGCCCTCC CAGAGCAGAG GCGGGGCGTC CGGGTAGCCG TGCAGCAGCT GCAGCAGGAG 
AAGGGTGGCC TGAGCTGGAC GCACGGACAG CCTCAGGCCC TGGAGATGCT GTTCCAGGAG 
TGCCAGGCTC TCCAGGCCCA GCTCCGACAG TCTAAGGCAT TGACCCAGGA GCCAGAAAGG 
GAGGACGCTC TTCAAGCTGA GCGGCACCCG TGGGAGCAGT TGAGGCAAGC AGCGGCTTCT 
GTCCATATGC TGGAAAACAG CCGGGATCTC TATAAGAAGA AGTACCGAGC GGCTCTGGGC 
CGAGTTGGTG AGCTAGAGAG CCAGGTGCTT CGTCTGCAAG AAGAGGTGGT CAATCTTTGG 
AGCAGCGGCC AAGCAGCGGG CAGCCTTGGG CAGCTGAGTG CTCGGGAGAT GGGCTGGGCG 
ATGCAGCTCA CAGGCCAGCA AGAGCAGACC ATCCGGGAGC TGCAGGGACA GTTGGCAGCC 
AGCCACAGGA AGTTGCTGCA GCAAAAGGAA GCCCTGGCCG CCCTGCGCCG AGAGTTTGCA 
TCCTACAAGT CGACTCACAG CTGCTCCAAC GCAAAGCACA GGAAACAGAG GCTCAGCCAG 
GCGATGCTCC GTCAGAAGCT CCAGCAGGCA GAAGAGGAGG GTTTGCAGCA GCAGGCGGAG 
GCCTACCAGG CCCTTGTGCA GGAACTGAAG CTGCAGCTGG CAAGGGAGGC CGAGCAGAAC 
AGCAGCACCT TGAAAGATCT GGCTCGCCTG GAACTGGCTG TGCAGAGCCT GTGCCAGGAG 
CCAGCGCCAG AGCAGAGTCG GCAGCTGCAG CACCACGCCT GCCCAGCGCA GGCCCTGCGG 
GAACAAAGCC ACCCTGTGCT GGGTCCACAG AAGGAATGGG AACCAGACAA GCTCCTTCAA 
AGGGCTAATT GCAGCCTCTT CTGGGACCAG GCCCATCCCT TGAATGCTGC CTCACGGGAG 
GCCTCTTGGC CTGGTGGGGC TCCAGTGCTG CAGCCGAGGA GGCCCGAGTG CCCGGAACAG 
CCCAGGAGGG CGGCACTGGA GGTGGCCCAG GACCAGGCGG TGGAGAGGGA CCTGGAGCTT 
CAGCGCCTTG GGGATGTTGG CTGCCCGCTG GAGGTTCTCC TTGGAAGAGG CTGCCTAGCT 
GGAGCAGAGC TGGAGCAGGA AAGCTTCTTG GGAGGAGCCT CTGGCAGAGA ACGGCTGAAG 
GCGCTGAGGG AGAAGCTGAG CAGGTCCAAG CAGCCGCCTT TGCAGGCAGC ATCCATGCAG 
GAGTTGGAGA TGCTGCGGCA GCAGTTACAG GAGACCGAGA GGCGAGGGCG GGCCCTGCAG 
GCCTCCGTGG CGGAGCAGAC GGCAGAGCGC CGTTCTCTGC GAGAGCAGCT GACCAAGCAG 
ACGGCTGTTC TGTGGCTGCT GCAGGGACAC TTCAGGCAGG CCCGGCTGCA GCTGAAGCAC 
CAGGCAACCG CTATAGAATC CTTACGAGTC TCCCTGGAGG CATCCCACGC CGGCCATCGC 
TGGCTGCACC AGGAGAGCGA ACTGGTGGTG GCCGATCTTT GCCAGTGGGT GAAGGAGCAA 
AAGCAGATGA ATGAGGAGCT GGGCCGCAAG CTCCGAACGC AGATCAAGCA AATTGCCCAG 
CTGACCGGCG AGAGAGACCT TCTGCATCAA CTATTAGAGA GGCTGCAAGA GGACAACCGG 
CGGCTGAAGA ACGAAGTCAA CGAGAAGCGC GTTGTATGTG AGCGCATCAA GGCTCTCCAC 
AACAATGATT TGGAGCCACG GGCTGCACTG CAACAACTCT GGCACATACT CCTGTTGTGA