LOC107361254 cDNA ORF clone, Tetranychus urticae(two-spotted spider mite)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107361254
Entrez Gene ID	107361254
Full Name	protein patched-like
Gene Type	protein-coding
Organism	Tetranychus urticae(two-spotted spider mite)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015928036.2	XP_015783522.1	protein patched
XM_015928036.1	XP_015783522.1	protein patched-like

Pathways from BioSystems
Gene Ontology

The following LOC107361254 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107361254 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTe17583	XM_015928036.2 Latest version!	Tetranychus urticae protein patched (LOC107361254), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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OTe17583	XM_015928036.1	Tetranychus urticae protein patched-like (LOC107361254), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107361254 ( XM_015928036.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015783522.1 Tetranychus urticae protein patched (LOC107361254), mRNA.

Price & Availability↑

CloneID	OTe17583
Clone ID Related Accession (Same CDS sequence)	XM_015928036.2 , XM_015928036.1
Accession Version	XM_015928036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	protein patched
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449935.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015928036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATTT TACTTTTTAG GACTTCTTTG GTTAATGCAT CAATTGCATT ACGCCAAATT 
AAAAAGGGTA GAGCTTCGGG AAACAAAGCT GCCTTGGTAA TAAGAAGTAA ATTTCAGAAA 
CATCTTTATG ATATTGGATG TGTAACTCAA CGAAATCCAG GAACTGTTTT ATTTATTGGG 
CTTTTAGCCC TTTTAAGTGC ACTAGTTGGA TTAAAATCTG CTAAAATTGA AACCAACATT 
GATGAGTTAT TGGTTGAAGC TGGCGGTAGA CTAGAAAAAG AAATTGATTA TGTCAAAGAA 
ACATTGGGAG ATGGTTTTGA AAACACGAAC CAAATGTTAA TTCAAACACC AAACAATGAC 
AATCAAAACA TTCTCCATCC AAGTGCCTTA CTTTCACATT TAGAAGTTTT GAAAGTTGGT 
ACTCAAGTCA GTGTGGATAT GTTTTCAACG ACATGGCGTT TAAAAGATAT ATGCTACACT 
TTGAGTATTC CAGCCTTTGA AGAGCAACTT ATAGATGCCG CTTTGGATAA ATTAATACCT 
TGCGCAATAA TAACACCCTT GGATTGTTTC TGGGAAGGTT CTAAATTATT AGGACCCGAG 
ATTACTATAC CTGTCATCAA TTTGAATTTT GCGTGGACCA CTTTTAATCC TCAATCTTTG 
ATAGAGTTGG CCAAAAATCG TACCCACTCA GGTCATGGAT CACATAATCG CTATCCATTT 
GAAACATTTG AAAATCTTAT GAGCAGGGCT GGTATATCAA CAGCTTACCA AGGACGTCCT 
TGTTTGAATC CAGCAGACGA AGAATGTCCG GAAAGTGCTC CAAATAAACA TACAAACCGT 
GAGCCGGATG TTAGTTATGA ACTGTCCGGA GGATGTTATG GTTTCGCCAC CAAATGGATG 
CACTGGCCGG AACAACTTAT TATTGGAGGA ATTAAGAAAA ACAAGAATGG TCGTATTGTG 
AAAGCCCATG CTCTTCAATC TATCATCCAA TTAATGGCGG AACAAGATAT GTTTGCTTAC 
TGGCAGGATA ATGCTCGTGT TCTTCATATT GATTGGACCA TCGAGAAAGC TCGTCAAGTG 
TTGGAAGCAT GGCAACGTAA ATTTGTGTCT GAAGTTGATC GCTTCATCAA AGAAAACTCA 
ATTGTTGATT ACAAAATTGA AAAATTTACC AATGTGGCCT TGCTGGATAT TATGAAAAAT 
TTTTCTATTC CTAACGCAAC CCATTTACTT ATTGGATACT TTTTATTTTT GATATTTACC 
GCTATATCTC GTATTAATAT CGGTAATCCA AGTTACTCAT TGAGTGCTCT TGGCATTTGT 
GGTGTTCTTC TTATTGTGCT CTCCATTGCT TCTGGCTTAG GATTTTGCTG TCTCCTTGGC 
TTTACATTTA ATGCATCAAC AACTCAGATT ATTCCATTTT TAGCCCTAGC GTTGGGGTCA 
GCGCCATTAT TTTTTTTGAT GGACGCCTAC GAAGAAATTA TTTGTAAAGA TACTCATTCA 
AAGAATCCAA TTGCGGATAC ACTTCAGCAA GCTGGATTTA GCATTTTACT CAATTCATTT 
TCCCTTTTAT GTGCACTTGG CGCTGCTACC ATAATTCCTA TTCCTGCATT AAGATATTTT 
GCTGCTCAAA ATGCCATTTT ACTCACATTC ATCACCATTT CTCTGTTGAC AATTTTTCCC 
GCCATTATGA GCTTGGATTT ACTGAGGAGA AATTTTTCGA TATCATCAAA AAGCAAGAAT 
GATTTTAGTC GGAATATTTC AACTGAAACT CCTTTGTACT ACAAATTAAC ATTGGACTAC 
TTCGTAGAAA ATATTTATTT ACCTTTATTC CATGGAAGAT TTTTTAAATT TTTGGTGATG 
ATTGTTAGCT GTGTAATTTA TCTGATTTGC CTGTCAGGTG TGCCTCAAGT GAAAGACGGA 
CTTGATCTTA CCGATATTGT TCCTCGTGAC ACAAGTGAAT ATCGATTCCT CCATAACCAA 
AAACAATATT TTAGTGTATA TAATATGCAT GCGATAACTC AAGGTAATTT TGAGTATCCC 
ACCCACCAAG CTCTTCTTAT TGATTACCAC AACGCATTCA CTCGAGTTCC AAGAATCATT 
AAAAATGATG ATGGTGGATT ACCCGAGTTC TGGTTAACCA TGTTCAGAGA TTGGCTGATC 
GGTTTGCAAA ATTCTTTTGA TAAAGATTGG AAAAACGGGG CGATCAGTCA AGAAAGATGG 
TACGATAATG CTTCGGCAGA AGGAATTTTA GGTTACAAGT TGCTCGTTCA AACTGGTAGG 
GTAGACAATC CTGTCGACAA AAGTCTTGTT ACGAAGACGA AACTCGTTGA TAGTCGAGGT 
ATCATCAATC CTAAAGCATT TTATAATTAT TTGACTGCCT GGGTCACCAA TGATGCGATT 
GCTTATTCAG CATCCCAAGC AAATTTTCAT CCAGAACCTC GTCAATGGAT TCATGTAGCA 
TCTGACTATG ATTTAAAAAT ACCAAAATCT CAGCCTTTAA CTTATGCTGA AATACCTTTT 
TACTTGCATG ATATGAACTC AACCGAGGAA ATTACCTCAA CAATTCAAGA AATTAGGACA 
ATTTGTAAAG TTTATGAGGA AAAAGGCCTG CCGAACTTCC CAACAGGACT TCCATTCAAA 
TATTGGGAAC AGTATATTCG TTTGAGACTT TCATTAGGTG CATCTTTGAT CGTCGCTTTT 
GCAGCTATTT TTGTTGCCTT TTGTGCTCTC TTGGTCAATT GTTGGGCCTC CTCGTTGTTA 
ATATTTGTTC TAGCTCTAAC TAGTGTCCAA ATTTTTGGAT TTATGGGCTT ACTAAAAATC 
AGGCTGAGTG CTATTCCAGC CGTTATTCTT ATTATTGCCG TTGGCAAAAG CATTCATATA 
CTTATTCATA TGCTCATGAG TTTCCTGACC AGTATGGGTA AACGAGATTA TCGGATCTCA 
GCCGCTCTTC ATCACATGTT CCCATCTTTA GTTCGTAGTG AGATATCAAC TTTCATACCA 
ATTGGCATGT TAGCATTTTC TGAATTTGAC TTCGTCAATA GAGACTTTTT TTACATTTTT 
TCTGGAGCGG TTGCCATCTC CAGTTGGAAT GGTCTTATCT TATATCCTTT ACTCTTATTT 
TACTTTGGTC CACCCGGCGA ACTCATTCCA ATTGAATATG AGAATCGAAT CTCAACTCCC 
TCGCCTCCTT GTTCACCTCC GGCAAAGAAG CGACACCATC ATAATTTAGT TTCATATTCA 
CGACGTATTT ATCCTCGTGT GCAATCCGAG ATATCCTTGT CAACTATTTC AGAAGAACCA 
CCTTCTTACA AATCATCTCA CGAAATTGTT GTTCAGCCTG AGTTGGTGGT GGAAACAACA 
ACTGTGACCA ATACTGGGCC ACATCAAATT CCAACTCAAA CTACCTCACA TTTACCTGAT 
GATAAATCTG GTATGCATTC ATCAGCCTCG TCTTCTGATC GTGAAAGTCC AGAAACTCCT 
CTGAGCCGAG ATAGCCCTAC ATCTTCAAGT GATAGCGGTG CTTCATCATT GAGTAGATCA 
AGCTCAACAA CCACAACTAT TTATCCTTTA TACAATCCTG GAAACTTGTA TTCATATCCA 
CCTCAAACAG TGACTACCAA AGTAACTACG ACGGCCAAGT TGAAAGTTGA ACTTCACGCT 
CCTTATCTAG ATCGGGATTC CAGTTCAAGT CGCAATAAAA ATAAAGATCG TAGACAAAAT 
CGCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015783522.1
CDS	100..3828
Translation	MNILLFRTSLVNASIALRQIKKGRASGNKAALVIRSKFQKHLYDIGCVTQRNPGTVLFIGLLALLSALVGLKSAKIETNIDELLVEAGGRLEKEIDYVKETLGDGFENTNQMLIQTPNNDNQNILHPSALLSHLEVLKVGTQVSVDMFSTTWRLKDICYTLSIPAFEEQLIDAALDKLIPCAIITPLDCFWEGSKLLGPEITIPVINLNFAWTTFNPQSLIELAKNRTHSGHGSHNRYPFETFENLMSRAGISTAYQGRPCLNPADEECPESAPNKHTNREPDVSYELSGGCYGFATKWMHWPEQLIIGGIKKNKNGRIVKAHALQSIIQLMAEQDMFAYWQDNARVLHIDWTIEKARQVLEAWQRKFVSEVDRFIKENSIVDYKIEKFTNVALLDIMKNFSIPNATHLLIGYFLFLIFTAISRINIGNPSYSLSALGICGVLLIVLSIASGLGFCCLLGFTFNASTTQIIPFLALALGSAPLFFLMDAYEEIICKDTHSKNPIADTLQQAGFSILLNSFSLLCALGAATIIPIPALRYFAAQNAILLTFITISLLTIFPAIMSLDLLRRNFSISSKSKNDFSRNISTETPLYYKLTLDYFVENIYLPLFHGRFFKFLVMIVSCVIYLICLSGVPQVKDGLDLTDIVPRDTSEYRFLHNQKQYFSVYNMHAITQGNFEYPTHQALLIDYHNAFTRVPRIIKNDDGGLPEFWLTMFRDWLIGLQNSFDKDWKNGAISQERWYDNASAEGILGYKLLVQTGRVDNPVDKSLVTKTKLVDSRGIINPKAFYNYLTAWVTNDAIAYSASQANFHPEPRQWIHVASDYDLKIPKSQPLTYAEIPFYLHDMNSTEEITSTIQEIRTICKVYEEKGLPNFPTGLPFKYWEQYIRLRLSLGASLIVAFAAIFVAFCALLVNCWASSLLIFVLALTSVQIFGFMGLLKIRLSAIPAVILIIAVGKSIHILIHMLMSFLTSMGKRDYRISAALHHMFPSLVRSEISTFIPIGMLAFSEFDFVNRDFFYIFSGAVAISSWNGLILYPLLLFYFGPPGELIPIEYENRISTPSPPCSPPAKKRHHHNLVSYSRRIYPRVQSEISLSTISEEPPSYKSSHEIVVQPELVVETTTVTNTGPHQIPTQTTSHLPDDKSGMHSSASSSDRESPETPLSRDSPTSSSDSGASSLSRSSSTTTTIYPLYNPGNLYSYPPQTVTTKVTTTAKLKVELHAPYLDRDSSSSRNKNKDRRQNRS

Target ORF information:

RefSeq Version	XM_015928036.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae protein patched (LOC107361254), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015928036.2

ORF Insert Sequence:

ATGAATATTT TACTTTTTAG GACTTCTTTG GTTAATGCAT CAATTGCATT ACGCCAAATT 
AAAAAGGGTA GAGCTTCGGG AAACAAAGCT GCCTTGGTAA TAAGAAGTAA ATTTCAGAAA 
CATCTTTATG ATATTGGATG TGTAACTCAA CGAAATCCAG GAACTGTTTT ATTTATTGGG 
CTTTTAGCCC TTTTAAGTGC ACTAGTTGGA TTAAAATCTG CTAAAATTGA AACCAACATT 
GATGAGTTAT TGGTTGAAGC TGGCGGTAGA CTAGAAAAAG AAATTGATTA TGTCAAAGAA 
ACATTGGGAG ATGGTTTTGA AAACACGAAC CAAATGTTAA TTCAAACACC AAACAATGAC 
AATCAAAACA TTCTCCATCC AAGTGCCTTA CTTTCACATT TAGAAGTTTT GAAAGTTGGT 
ACTCAAGTCA GTGTGGATAT GTTTTCAACG ACATGGCGTT TAAAAGATAT ATGCTACACT 
TTGAGTATTC CAGCCTTTGA AGAGCAACTT ATAGATGCCG CTTTGGATAA ATTAATACCT 
TGCGCAATAA TAACACCCTT GGATTGTTTC TGGGAAGGTT CTAAATTATT AGGACCCGAG 
ATTACTATAC CTGTCATCAA TTTGAATTTT GCGTGGACCA CTTTTAATCC TCAATCTTTG 
ATAGAGTTGG CCAAAAATCG TACCCACTCA GGTCATGGAT CACATAATCG CTATCCATTT 
GAAACATTTG AAAATCTTAT GAGCAGGGCT GGTATATCAA CAGCTTACCA AGGACGTCCT 
TGTTTGAATC CAGCAGACGA AGAATGTCCG GAAAGTGCTC CAAATAAACA TACAAACCGT 
GAGCCGGATG TTAGTTATGA ACTGTCCGGA GGATGTTATG GTTTCGCCAC CAAATGGATG 
CACTGGCCGG AACAACTTAT TATTGGAGGA ATTAAGAAAA ACAAGAATGG TCGTATTGTG 
AAAGCCCATG CTCTTCAATC TATCATCCAA TTAATGGCGG AACAAGATAT GTTTGCTTAC 
TGGCAGGATA ATGCTCGTGT TCTTCATATT GATTGGACCA TCGAGAAAGC TCGTCAAGTG 
TTGGAAGCAT GGCAACGTAA ATTTGTGTCT GAAGTTGATC GCTTCATCAA AGAAAACTCA 
ATTGTTGATT ACAAAATTGA AAAATTTACC AATGTGGCCT TGCTGGATAT TATGAAAAAT 
TTTTCTATTC CTAACGCAAC CCATTTACTT ATTGGATACT TTTTATTTTT GATATTTACC 
GCTATATCTC GTATTAATAT CGGTAATCCA AGTTACTCAT TGAGTGCTCT TGGCATTTGT 
GGTGTTCTTC TTATTGTGCT CTCCATTGCT TCTGGCTTAG GATTTTGCTG TCTCCTTGGC 
TTTACATTTA ATGCATCAAC AACTCAGATT ATTCCATTTT TAGCCCTAGC GTTGGGGTCA 
GCGCCATTAT TTTTTTTGAT GGACGCCTAC GAAGAAATTA TTTGTAAAGA TACTCATTCA 
AAGAATCCAA TTGCGGATAC ACTTCAGCAA GCTGGATTTA GCATTTTACT CAATTCATTT 
TCCCTTTTAT GTGCACTTGG CGCTGCTACC ATAATTCCTA TTCCTGCATT AAGATATTTT 
GCTGCTCAAA ATGCCATTTT ACTCACATTC ATCACCATTT CTCTGTTGAC AATTTTTCCC 
GCCATTATGA GCTTGGATTT ACTGAGGAGA AATTTTTCGA TATCATCAAA AAGCAAGAAT 
GATTTTAGTC GGAATATTTC AACTGAAACT CCTTTGTACT ACAAATTAAC ATTGGACTAC 
TTCGTAGAAA ATATTTATTT ACCTTTATTC CATGGAAGAT TTTTTAAATT TTTGGTGATG 
ATTGTTAGCT GTGTAATTTA TCTGATTTGC CTGTCAGGTG TGCCTCAAGT GAAAGACGGA 
CTTGATCTTA CCGATATTGT TCCTCGTGAC ACAAGTGAAT ATCGATTCCT CCATAACCAA 
AAACAATATT TTAGTGTATA TAATATGCAT GCGATAACTC AAGGTAATTT TGAGTATCCC 
ACCCACCAAG CTCTTCTTAT TGATTACCAC AACGCATTCA CTCGAGTTCC AAGAATCATT 
AAAAATGATG ATGGTGGATT ACCCGAGTTC TGGTTAACCA TGTTCAGAGA TTGGCTGATC 
GGTTTGCAAA ATTCTTTTGA TAAAGATTGG AAAAACGGGG CGATCAGTCA AGAAAGATGG 
TACGATAATG CTTCGGCAGA AGGAATTTTA GGTTACAAGT TGCTCGTTCA AACTGGTAGG 
GTAGACAATC CTGTCGACAA AAGTCTTGTT ACGAAGACGA AACTCGTTGA TAGTCGAGGT 
ATCATCAATC CTAAAGCATT TTATAATTAT TTGACTGCCT GGGTCACCAA TGATGCGATT 
GCTTATTCAG CATCCCAAGC AAATTTTCAT CCAGAACCTC GTCAATGGAT TCATGTAGCA 
TCTGACTATG ATTTAAAAAT ACCAAAATCT CAGCCTTTAA CTTATGCTGA AATACCTTTT 
TACTTGCATG ATATGAACTC AACCGAGGAA ATTACCTCAA CAATTCAAGA AATTAGGACA 
ATTTGTAAAG TTTATGAGGA AAAAGGCCTG CCGAACTTCC CAACAGGACT TCCATTCAAA 
TATTGGGAAC AGTATATTCG TTTGAGACTT TCATTAGGTG CATCTTTGAT CGTCGCTTTT 
GCAGCTATTT TTGTTGCCTT TTGTGCTCTC TTGGTCAATT GTTGGGCCTC CTCGTTGTTA 
ATATTTGTTC TAGCTCTAAC TAGTGTCCAA ATTTTTGGAT TTATGGGCTT ACTAAAAATC 
AGGCTGAGTG CTATTCCAGC CGTTATTCTT ATTATTGCCG TTGGCAAAAG CATTCATATA 
CTTATTCATA TGCTCATGAG TTTCCTGACC AGTATGGGTA AACGAGATTA TCGGATCTCA 
GCCGCTCTTC ATCACATGTT CCCATCTTTA GTTCGTAGTG AGATATCAAC TTTCATACCA 
ATTGGCATGT TAGCATTTTC TGAATTTGAC TTCGTCAATA GAGACTTTTT TTACATTTTT 
TCTGGAGCGG TTGCCATCTC CAGTTGGAAT GGTCTTATCT TATATCCTTT ACTCTTATTT 
TACTTTGGTC CACCCGGCGA ACTCATTCCA ATTGAATATG AGAATCGAAT CTCAACTCCC 
TCGCCTCCTT GTTCACCTCC GGCAAAGAAG CGACACCATC ATAATTTAGT TTCATATTCA 
CGACGTATTT ATCCTCGTGT GCAATCCGAG ATATCCTTGT CAACTATTTC AGAAGAACCA 
CCTTCTTACA AATCATCTCA CGAAATTGTT GTTCAGCCTG AGTTGGTGGT GGAAACAACA 
ACTGTGACCA ATACTGGGCC ACATCAAATT CCAACTCAAA CTACCTCACA TTTACCTGAT 
GATAAATCTG GTATGCATTC ATCAGCCTCG TCTTCTGATC GTGAAAGTCC AGAAACTCCT 
CTGAGCCGAG ATAGCCCTAC ATCTTCAAGT GATAGCGGTG CTTCATCATT GAGTAGATCA 
AGCTCAACAA CCACAACTAT TTATCCTTTA TACAATCCTG GAAACTTGTA TTCATATCCA 
CCTCAAACAG TGACTACCAA AGTAACTACG ACGGCCAAGT TGAAAGTTGA ACTTCACGCT 
CCTTATCTAG ATCGGGATTC CAGTTCAAGT CGCAATAAAA ATAAAGATCG TAGACAAAAT 
CGCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107361254 ( XM_015928036.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015783522.1 Tetranychus urticae protein patched-like (LOC107361254), mRNA.

Price & Availability↑

CloneID	OTe17583
Clone ID Related Accession (Same CDS sequence)	XM_015928036.2 , XM_015928036.1
Accession Version	XM_015928036.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	protein patched-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449935.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATTT TACTTTTTAG GACTTCTTTG GTTAATGCAT CAATTGCATT ACGCCAAATT 
AAAAAGGGTA GAGCTTCGGG AAACAAAGCT GCCTTGGTAA TAAGAAGTAA ATTTCAGAAA 
CATCTTTATG ATATTGGATG TGTAACTCAA CGAAATCCAG GAACTGTTTT ATTTATTGGG 
CTTTTAGCCC TTTTAAGTGC ACTAGTTGGA TTAAAATCTG CTAAAATTGA AACCAACATT 
GATGAGTTAT TGGTTGAAGC TGGCGGTAGA CTAGAAAAAG AAATTGATTA TGTCAAAGAA 
ACATTGGGAG ATGGTTTTGA AAACACGAAC CAAATGTTAA TTCAAACACC AAACAATGAC 
AATCAAAACA TTCTCCATCC AAGTGCCTTA CTTTCACATT TAGAAGTTTT GAAAGTTGGT 
ACTCAAGTCA GTGTGGATAT GTTTTCAACG ACATGGCGTT TAAAAGATAT ATGCTACACT 
TTGAGTATTC CAGCCTTTGA AGAGCAACTT ATAGATGCCG CTTTGGATAA ATTAATACCT 
TGCGCAATAA TAACACCCTT GGATTGTTTC TGGGAAGGTT CTAAATTATT AGGACCCGAG 
ATTACTATAC CTGTCATCAA TTTGAATTTT GCGTGGACCA CTTTTAATCC TCAATCTTTG 
ATAGAGTTGG CCAAAAATCG TACCCACTCA GGTCATGGAT CACATAATCG CTATCCATTT 
GAAACATTTG AAAATCTTAT GAGCAGGGCT GGTATATCAA CAGCTTACCA AGGACGTCCT 
TGTTTGAATC CAGCAGACGA AGAATGTCCG GAAAGTGCTC CAAATAAACA TACAAACCGT 
GAGCCGGATG TTAGTTATGA ACTGTCCGGA GGATGTTATG GTTTCGCCAC CAAATGGATG 
CACTGGCCGG AACAACTTAT TATTGGAGGA ATTAAGAAAA ACAAGAATGG TCGTATTGTG 
AAAGCCCATG CTCTTCAATC TATCATCCAA TTAATGGCGG AACAAGATAT GTTTGCTTAC 
TGGCAGGATA ATGCTCGTGT TCTTCATATT GATTGGACCA TCGAGAAAGC TCGTCAAGTG 
TTGGAAGCAT GGCAACGTAA ATTTGTGTCT GAAGTTGATC GCTTCATCAA AGAAAACTCA 
ATTGTTGATT ACAAAATTGA AAAATTTACC AATGTGGCCT TGCTGGATAT TATGAAAAAT 
TTTTCTATTC CTAACGCAAC CCATTTACTT ATTGGATACT TTTTATTTTT GATATTTACC 
GCTATATCTC GTATTAATAT CGGTAATCCA AGTTACTCAT TGAGTGCTCT TGGCATTTGT 
GGTGTTCTTC TTATTGTGCT CTCCATTGCT TCTGGCTTAG GATTTTGCTG TCTCCTTGGC 
TTTACATTTA ATGCATCAAC AACTCAGATT ATTCCATTTT TAGCCCTAGC GTTGGGGTCA 
GCGCCATTAT TTTTTTTGAT GGACGCCTAC GAAGAAATTA TTTGTAAAGA TACTCATTCA 
AAGAATCCAA TTGCGGATAC ACTTCAGCAA GCTGGATTTA GCATTTTACT CAATTCATTT 
TCCCTTTTAT GTGCACTTGG CGCTGCTACC ATAATTCCTA TTCCTGCATT AAGATATTTT 
GCTGCTCAAA ATGCCATTTT ACTCACATTC ATCACCATTT CTCTGTTGAC AATTTTTCCC 
GCCATTATGA GCTTGGATTT ACTGAGGAGA AATTTTTCGA TATCATCAAA AAGCAAGAAT 
GATTTTAGTC GGAATATTTC AACTGAAACT CCTTTGTACT ACAAATTAAC ATTGGACTAC 
TTCGTAGAAA ATATTTATTT ACCTTTATTC CATGGAAGAT TTTTTAAATT TTTGGTGATG 
ATTGTTAGCT GTGTAATTTA TCTGATTTGC CTGTCAGGTG TGCCTCAAGT GAAAGACGGA 
CTTGATCTTA CCGATATTGT TCCTCGTGAC ACAAGTGAAT ATCGATTCCT CCATAACCAA 
AAACAATATT TTAGTGTATA TAATATGCAT GCGATAACTC AAGGTAATTT TGAGTATCCC 
ACCCACCAAG CTCTTCTTAT TGATTACCAC AACGCATTCA CTCGAGTTCC AAGAATCATT 
AAAAATGATG ATGGTGGATT ACCCGAGTTC TGGTTAACCA TGTTCAGAGA TTGGCTGATC 
GGTTTGCAAA ATTCTTTTGA TAAAGATTGG AAAAACGGGG CGATCAGTCA AGAAAGATGG 
TACGATAATG CTTCGGCAGA AGGAATTTTA GGTTACAAGT TGCTCGTTCA AACTGGTAGG 
GTAGACAATC CTGTCGACAA AAGTCTTGTT ACGAAGACGA AACTCGTTGA TAGTCGAGGT 
ATCATCAATC CTAAAGCATT TTATAATTAT TTGACTGCCT GGGTCACCAA TGATGCGATT 
GCTTATTCAG CATCCCAAGC AAATTTTCAT CCAGAACCTC GTCAATGGAT TCATGTAGCA 
TCTGACTATG ATTTAAAAAT ACCAAAATCT CAGCCTTTAA CTTATGCTGA AATACCTTTT 
TACTTGCATG ATATGAACTC AACCGAGGAA ATTACCTCAA CAATTCAAGA AATTAGGACA 
ATTTGTAAAG TTTATGAGGA AAAAGGCCTG CCGAACTTCC CAACAGGACT TCCATTCAAA 
TATTGGGAAC AGTATATTCG TTTGAGACTT TCATTAGGTG CATCTTTGAT CGTCGCTTTT 
GCAGCTATTT TTGTTGCCTT TTGTGCTCTC TTGGTCAATT GTTGGGCCTC CTCGTTGTTA 
ATATTTGTTC TAGCTCTAAC TAGTGTCCAA ATTTTTGGAT TTATGGGCTT ACTAAAAATC 
AGGCTGAGTG CTATTCCAGC CGTTATTCTT ATTATTGCCG TTGGCAAAAG CATTCATATA 
CTTATTCATA TGCTCATGAG TTTCCTGACC AGTATGGGTA AACGAGATTA TCGGATCTCA 
GCCGCTCTTC ATCACATGTT CCCATCTTTA GTTCGTAGTG AGATATCAAC TTTCATACCA 
ATTGGCATGT TAGCATTTTC TGAATTTGAC TTCGTCAATA GAGACTTTTT TTACATTTTT 
TCTGGAGCGG TTGCCATCTC CAGTTGGAAT GGTCTTATCT TATATCCTTT ACTCTTATTT 
TACTTTGGTC CACCCGGCGA ACTCATTCCA ATTGAATATG AGAATCGAAT CTCAACTCCC 
TCGCCTCCTT GTTCACCTCC GGCAAAGAAG CGACACCATC ATAATTTAGT TTCATATTCA 
CGACGTATTT ATCCTCGTGT GCAATCCGAG ATATCCTTGT CAACTATTTC AGAAGAACCA 
CCTTCTTACA AATCATCTCA CGAAATTGTT GTTCAGCCTG AGTTGGTGGT GGAAACAACA 
ACTGTGACCA ATACTGGGCC ACATCAAATT CCAACTCAAA CTACCTCACA TTTACCTGAT 
GATAAATCTG GTATGCATTC ATCAGCCTCG TCTTCTGATC GTGAAAGTCC AGAAACTCCT 
CTGAGCCGAG ATAGCCCTAC ATCTTCAAGT GATAGCGGTG CTTCATCATT GAGTAGATCA 
AGCTCAACAA CCACAACTAT TTATCCTTTA TACAATCCTG GAAACTTGTA TTCATATCCA 
CCTCAAACAG TGACTACCAA AGTAACTACG ACGGCCAAGT TGAAAGTTGA ACTTCACGCT 
CCTTATCTAG ATCGGGATTC CAGTTCAAGT CGCAATAAAA ATAAAGATCG TAGACAAAAT 
CGCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015783522.1
CDS	84..3812
Translation	MNILLFRTSLVNASIALRQIKKGRASGNKAALVIRSKFQKHLYDIGCVTQRNPGTVLFIGLLALLSALVGLKSAKIETNIDELLVEAGGRLEKEIDYVKETLGDGFENTNQMLIQTPNNDNQNILHPSALLSHLEVLKVGTQVSVDMFSTTWRLKDICYTLSIPAFEEQLIDAALDKLIPCAIITPLDCFWEGSKLLGPEITIPVINLNFAWTTFNPQSLIELAKNRTHSGHGSHNRYPFETFENLMSRAGISTAYQGRPCLNPADEECPESAPNKHTNREPDVSYELSGGCYGFATKWMHWPEQLIIGGIKKNKNGRIVKAHALQSIIQLMAEQDMFAYWQDNARVLHIDWTIEKARQVLEAWQRKFVSEVDRFIKENSIVDYKIEKFTNVALLDIMKNFSIPNATHLLIGYFLFLIFTAISRINIGNPSYSLSALGICGVLLIVLSIASGLGFCCLLGFTFNASTTQIIPFLALALGSAPLFFLMDAYEEIICKDTHSKNPIADTLQQAGFSILLNSFSLLCALGAATIIPIPALRYFAAQNAILLTFITISLLTIFPAIMSLDLLRRNFSISSKSKNDFSRNISTETPLYYKLTLDYFVENIYLPLFHGRFFKFLVMIVSCVIYLICLSGVPQVKDGLDLTDIVPRDTSEYRFLHNQKQYFSVYNMHAITQGNFEYPTHQALLIDYHNAFTRVPRIIKNDDGGLPEFWLTMFRDWLIGLQNSFDKDWKNGAISQERWYDNASAEGILGYKLLVQTGRVDNPVDKSLVTKTKLVDSRGIINPKAFYNYLTAWVTNDAIAYSASQANFHPEPRQWIHVASDYDLKIPKSQPLTYAEIPFYLHDMNSTEEITSTIQEIRTICKVYEEKGLPNFPTGLPFKYWEQYIRLRLSLGASLIVAFAAIFVAFCALLVNCWASSLLIFVLALTSVQIFGFMGLLKIRLSAIPAVILIIAVGKSIHILIHMLMSFLTSMGKRDYRISAALHHMFPSLVRSEISTFIPIGMLAFSEFDFVNRDFFYIFSGAVAISSWNGLILYPLLLFYFGPPGELIPIEYENRISTPSPPCSPPAKKRHHHNLVSYSRRIYPRVQSEISLSTISEEPPSYKSSHEIVVQPELVVETTTVTNTGPHQIPTQTTSHLPDDKSGMHSSASSSDRESPETPLSRDSPTSSSDSGASSLSRSSSTTTTIYPLYNPGNLYSYPPQTVTTKVTTTAKLKVELHAPYLDRDSSSSRNKNKDRRQNRS

Target ORF information:

RefSeq Version	XM_015928036.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae protein patched-like (LOC107361254), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015928036.1

ORF Insert Sequence:

ATGAATATTT TACTTTTTAG GACTTCTTTG GTTAATGCAT CAATTGCATT ACGCCAAATT 
AAAAAGGGTA GAGCTTCGGG AAACAAAGCT GCCTTGGTAA TAAGAAGTAA ATTTCAGAAA 
CATCTTTATG ATATTGGATG TGTAACTCAA CGAAATCCAG GAACTGTTTT ATTTATTGGG 
CTTTTAGCCC TTTTAAGTGC ACTAGTTGGA TTAAAATCTG CTAAAATTGA AACCAACATT 
GATGAGTTAT TGGTTGAAGC TGGCGGTAGA CTAGAAAAAG AAATTGATTA TGTCAAAGAA 
ACATTGGGAG ATGGTTTTGA AAACACGAAC CAAATGTTAA TTCAAACACC AAACAATGAC 
AATCAAAACA TTCTCCATCC AAGTGCCTTA CTTTCACATT TAGAAGTTTT GAAAGTTGGT 
ACTCAAGTCA GTGTGGATAT GTTTTCAACG ACATGGCGTT TAAAAGATAT ATGCTACACT 
TTGAGTATTC CAGCCTTTGA AGAGCAACTT ATAGATGCCG CTTTGGATAA ATTAATACCT 
TGCGCAATAA TAACACCCTT GGATTGTTTC TGGGAAGGTT CTAAATTATT AGGACCCGAG 
ATTACTATAC CTGTCATCAA TTTGAATTTT GCGTGGACCA CTTTTAATCC TCAATCTTTG 
ATAGAGTTGG CCAAAAATCG TACCCACTCA GGTCATGGAT CACATAATCG CTATCCATTT 
GAAACATTTG AAAATCTTAT GAGCAGGGCT GGTATATCAA CAGCTTACCA AGGACGTCCT 
TGTTTGAATC CAGCAGACGA AGAATGTCCG GAAAGTGCTC CAAATAAACA TACAAACCGT 
GAGCCGGATG TTAGTTATGA ACTGTCCGGA GGATGTTATG GTTTCGCCAC CAAATGGATG 
CACTGGCCGG AACAACTTAT TATTGGAGGA ATTAAGAAAA ACAAGAATGG TCGTATTGTG 
AAAGCCCATG CTCTTCAATC TATCATCCAA TTAATGGCGG AACAAGATAT GTTTGCTTAC 
TGGCAGGATA ATGCTCGTGT TCTTCATATT GATTGGACCA TCGAGAAAGC TCGTCAAGTG 
TTGGAAGCAT GGCAACGTAA ATTTGTGTCT GAAGTTGATC GCTTCATCAA AGAAAACTCA 
ATTGTTGATT ACAAAATTGA AAAATTTACC AATGTGGCCT TGCTGGATAT TATGAAAAAT 
TTTTCTATTC CTAACGCAAC CCATTTACTT ATTGGATACT TTTTATTTTT GATATTTACC 
GCTATATCTC GTATTAATAT CGGTAATCCA AGTTACTCAT TGAGTGCTCT TGGCATTTGT 
GGTGTTCTTC TTATTGTGCT CTCCATTGCT TCTGGCTTAG GATTTTGCTG TCTCCTTGGC 
TTTACATTTA ATGCATCAAC AACTCAGATT ATTCCATTTT TAGCCCTAGC GTTGGGGTCA 
GCGCCATTAT TTTTTTTGAT GGACGCCTAC GAAGAAATTA TTTGTAAAGA TACTCATTCA 
AAGAATCCAA TTGCGGATAC ACTTCAGCAA GCTGGATTTA GCATTTTACT CAATTCATTT 
TCCCTTTTAT GTGCACTTGG CGCTGCTACC ATAATTCCTA TTCCTGCATT AAGATATTTT 
GCTGCTCAAA ATGCCATTTT ACTCACATTC ATCACCATTT CTCTGTTGAC AATTTTTCCC 
GCCATTATGA GCTTGGATTT ACTGAGGAGA AATTTTTCGA TATCATCAAA AAGCAAGAAT 
GATTTTAGTC GGAATATTTC AACTGAAACT CCTTTGTACT ACAAATTAAC ATTGGACTAC 
TTCGTAGAAA ATATTTATTT ACCTTTATTC CATGGAAGAT TTTTTAAATT TTTGGTGATG 
ATTGTTAGCT GTGTAATTTA TCTGATTTGC CTGTCAGGTG TGCCTCAAGT GAAAGACGGA 
CTTGATCTTA CCGATATTGT TCCTCGTGAC ACAAGTGAAT ATCGATTCCT CCATAACCAA 
AAACAATATT TTAGTGTATA TAATATGCAT GCGATAACTC AAGGTAATTT TGAGTATCCC 
ACCCACCAAG CTCTTCTTAT TGATTACCAC AACGCATTCA CTCGAGTTCC AAGAATCATT 
AAAAATGATG ATGGTGGATT ACCCGAGTTC TGGTTAACCA TGTTCAGAGA TTGGCTGATC 
GGTTTGCAAA ATTCTTTTGA TAAAGATTGG AAAAACGGGG CGATCAGTCA AGAAAGATGG 
TACGATAATG CTTCGGCAGA AGGAATTTTA GGTTACAAGT TGCTCGTTCA AACTGGTAGG 
GTAGACAATC CTGTCGACAA AAGTCTTGTT ACGAAGACGA AACTCGTTGA TAGTCGAGGT 
ATCATCAATC CTAAAGCATT TTATAATTAT TTGACTGCCT GGGTCACCAA TGATGCGATT 
GCTTATTCAG CATCCCAAGC AAATTTTCAT CCAGAACCTC GTCAATGGAT TCATGTAGCA 
TCTGACTATG ATTTAAAAAT ACCAAAATCT CAGCCTTTAA CTTATGCTGA AATACCTTTT 
TACTTGCATG ATATGAACTC AACCGAGGAA ATTACCTCAA CAATTCAAGA AATTAGGACA 
ATTTGTAAAG TTTATGAGGA AAAAGGCCTG CCGAACTTCC CAACAGGACT TCCATTCAAA 
TATTGGGAAC AGTATATTCG TTTGAGACTT TCATTAGGTG CATCTTTGAT CGTCGCTTTT 
GCAGCTATTT TTGTTGCCTT TTGTGCTCTC TTGGTCAATT GTTGGGCCTC CTCGTTGTTA 
ATATTTGTTC TAGCTCTAAC TAGTGTCCAA ATTTTTGGAT TTATGGGCTT ACTAAAAATC 
AGGCTGAGTG CTATTCCAGC CGTTATTCTT ATTATTGCCG TTGGCAAAAG CATTCATATA 
CTTATTCATA TGCTCATGAG TTTCCTGACC AGTATGGGTA AACGAGATTA TCGGATCTCA 
GCCGCTCTTC ATCACATGTT CCCATCTTTA GTTCGTAGTG AGATATCAAC TTTCATACCA 
ATTGGCATGT TAGCATTTTC TGAATTTGAC TTCGTCAATA GAGACTTTTT TTACATTTTT 
TCTGGAGCGG TTGCCATCTC CAGTTGGAAT GGTCTTATCT TATATCCTTT ACTCTTATTT 
TACTTTGGTC CACCCGGCGA ACTCATTCCA ATTGAATATG AGAATCGAAT CTCAACTCCC 
TCGCCTCCTT GTTCACCTCC GGCAAAGAAG CGACACCATC ATAATTTAGT TTCATATTCA 
CGACGTATTT ATCCTCGTGT GCAATCCGAG ATATCCTTGT CAACTATTTC AGAAGAACCA 
CCTTCTTACA AATCATCTCA CGAAATTGTT GTTCAGCCTG AGTTGGTGGT GGAAACAACA 
ACTGTGACCA ATACTGGGCC ACATCAAATT CCAACTCAAA CTACCTCACA TTTACCTGAT 
GATAAATCTG GTATGCATTC ATCAGCCTCG TCTTCTGATC GTGAAAGTCC AGAAACTCCT 
CTGAGCCGAG ATAGCCCTAC ATCTTCAAGT GATAGCGGTG CTTCATCATT GAGTAGATCA 
AGCTCAACAA CCACAACTAT TTATCCTTTA TACAATCCTG GAAACTTGTA TTCATATCCA 
CCTCAAACAG TGACTACCAA AGTAACTACG ACGGCCAAGT TGAAAGTTGA ACTTCACGCT 
CCTTATCTAG ATCGGGATTC CAGTTCAAGT CGCAATAAAA ATAAAGATCG TAGACAAAAT 
CGCTCTTGA