NPPB cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPPB
Entrez Gene ID	396844
Full Name	natriuretic peptide B
Synonyms	BNP,BNP1
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 6 Map Location: 6\|6

mRNA and Protein(s)

mRNA	Protein	Name
NM_213846.1	NP_999011.1	natriuretic peptides B precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Brain natriuretic peptide and C-type natriuretic peptide maintain porcine oocyte meiotic arrest.
Zhang W, Yang Y, Liu W, Chen Q, Wang H, Wang X, Zhang Y, Zhang M, Xia G
Journal of cellular physiology230(1)71-81(2015 Jan)

Cardiac natriuretic Peptide gene expression and plasma concentrations during the first 72 hours of life in piglets.
Smith J, Christoffersen C, N?rgaard LM, Olsen LH, Vejlstrup NG, Andersen CB, Goetze JP
Endocrinology154(5)1864-72(2013 May)

Development of porcine model of chronic tachycardia-induced cardiomyopathy.
Paslawska U, Gajek J, Kiczak L, Noszczyk-Nowak A, Skrzypczak P, Bania J, Tomaszek A, Zacharski M, Sambor I, Dziegiel P, Zysko D, Banasiak W, Jankowska EA, Ponikowski P
International journal of cardiology153(1)36-41(2011 Nov)

B-type natriuretic peptide and high sensitive C-reactive protein predict 2-year all cause mortality in chest pain patients: a prospective observational study from Salta, Argentina.
Le?n de la Fuente R, Naesgaard PA, Nilsen ST, Woie L, Aarsland T, Gallo P, Grundt H, Staines H, Nilsen DW
BMC cardiovascular disorders1157(2011 Sep)

Signaling molecules for early detection of adverse interactions during mechanical ventilation in animal models.
Kobr J, Pizingerova K, Fremuth J, Sasek L, Kocova J, Hes O, Racek J, Topolcan O
In vivo (Athens, Greece)25(2)209-17(2011 Mar-Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CNP and BNP serve as oocyte maturation inhibitor to inhibit oocyte maturation in mammals
Title: Brain natriuretic peptide and C-type natriuretic peptide maintain porcine oocyte meiotic arrest.

ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. The cardiac gene expression does not explain plasma concentrations.
Title: Cardiac natriuretic Peptide gene expression and plasma concentrations during the first 72 hours of life in piglets.

Up-regulation of BNP and a reduction in a ratio of sarcoplasmic reticulum calcium ATPase 2a in failing myocardium are observed in a porcine model of tachycardia.
Title: Development of porcine model of chronic tachycardia-induced cardiomyopathy.

serum levels predict mortality in chest pain patients in Argentina
Title: B-type natriuretic peptide and high sensitive C-reactive protein predict 2-year all cause mortality in chest pain patients: a prospective observational study from Salta, Argentina.

Data show that all five molecules, BNP, ICAM-1, TNF-alpha, VCAM-1 and IL-6, quickly and reliably signaled adverse interactions.
Title: Signaling molecules for early detection of adverse interactions during mechanical ventilation in animal models.

The following NPPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00068	NM_213846.1 Latest version!	Sus scrofa natriuretic peptide B (NPPB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPPB ( NM_213846.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999011.1 Sus scrofa natriuretic peptide B (NPPB), mRNA.

Price & Availability↑

CloneID	OSe00068
Clone ID Related Accession (Same CDS sequence)	NM_213846.1
Accession Version	NM_213846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Sus scrofa(pig)
Product	natriuretic peptides B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M23596.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M23596.1, BX915429.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1876852, SAMEA3180000 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCCCC GGATGGCGCT TCCCCGCGTG CTCCTGCTCC TGTTCTTGCA CCTGTTGCTG 
CTAGGATGCC GTTCCTATCC ACTGGGTGGC GCTGGCCTGG CCTCAGAACT GCCAGGGATA 
CAGGAGCTGC TGGACCGCCT GCGAGACAGG GTCTCCGAGC TGCAGGCGGA GCGGACGGAC 
CTGGAGCCCC TCCGGCAGGA CCGTGGCCTC ACAGAAGCCT GGGAGGCGAG GGAAGCAGCC 
CCCACGGGGG TTCTTGGGCC CCGCAGTAGC ATCTTCCAAG TCCTCCGGGG AATACGCAGC 
CCCAAGACGA TGCGTGACTC TGGCTGCTTT GGGCGGAGGC TGGACCGGAT CGGCTCCCTC 
AGCGGCCTGG GCTGCAATGT GCTCAGGAGG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999011.1
CDS	94..489
Translation	MGPRMALPRVLLLLFLHLLLLGCRSYPLGGAGLASELPGIQELLDRLRDRVSELQAERTDLEPLRQDRGLTEAWEAREAAPTGVLGPRSSIFQVLRGIRSPKTMRDSGCFGRRLDRIGSLSGLGCNVLRRY

Target ORF information:

RefSeq Version	NM_213846.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa natriuretic peptide B (NPPB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213846.1

ORF Insert Sequence:

ATGGGCCCCC GGATGGCGCT TCCCCGCGTG CTCCTGCTCC TGTTCTTGCA CCTGTTGCTG 
CTAGGATGCC GTTCCTATCC ACTGGGTGGC GCTGGCCTGG CCTCAGAACT GCCAGGGATA 
CAGGAGCTGC TGGACCGCCT GCGAGACAGG GTCTCCGAGC TGCAGGCGGA GCGGACGGAC 
CTGGAGCCCC TCCGGCAGGA CCGTGGCCTC ACAGAAGCCT GGGAGGCGAG GGAAGCAGCC 
CCCACGGGGG TTCTTGGGCC CCGCAGTAGC ATCTTCCAAG TCCTCCGGGG AATACGCAGC 
CCCAAGACGA TGCGTGACTC TGGCTGCTTT GGGCGGAGGC TGGACCGGAT CGGCTCCCTC 
AGCGGCCTGG GCTGCAATGT GCTCAGGAGG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Brain natriuretic peptide and C-type natriuretic peptide maintain porcine oocyte meiotic arrest.
Journal of cellular physiology230(1)71-81(2015 Jan)
Zhang W,Yang Y,Liu W,Chen Q,Wang H,Wang X,Zhang Y,Zhang M,Xia G

Cardiac natriuretic Peptide gene expression and plasma concentrations during the first 72 hours of life in piglets.
Endocrinology154(5)1864-72(2013 May)
Smith J,Christoffersen C,N?rgaard LM,Olsen LH,Vejlstrup NG,Andersen CB,Goetze JP

Development of porcine model of chronic tachycardia-induced cardiomyopathy.
International journal of cardiology153(1)36-41(2011 Nov)
Paslawska U,Gajek J,Kiczak L,Noszczyk-Nowak A,Skrzypczak P,Bania J,Tomaszek A,Zacharski M,Sambor I,Dziegiel P,Zysko D,Banasiak W,Jankowska EA,Ponikowski P

B-type natriuretic peptide and high sensitive C-reactive protein predict 2-year all cause mortality in chest pain patients: a prospective observational study from Salta, Argentina.
BMC cardiovascular disorders1157(2011 Sep)
Le?n de la Fuente R,Naesgaard PA,Nilsen ST,Woie L,Aarsland T,Gallo P,Grundt H,Staines H,Nilsen DW

Signaling molecules for early detection of adverse interactions during mechanical ventilation in animal models.
In vivo (Athens, Greece)25(2)209-17(2011 Mar-Apr)
Kobr J,Pizingerova K,Fremuth J,Sasek L,Kocova J,Hes O,Racek J,Topolcan O