LEP cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	LEP
Entrez Gene ID	396832
Full Name	leptin
Synonyms	OB,OBS
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 18 Map Location: 18\|18

mRNA and Protein(s)

mRNA	Protein	Name
XM_021078499.1	XP_020934158.1	leptin isoform X1
XM_021078500.1	XP_020934159.1	leptin isoform X2
XM_021078501.1	XP_020934160.1	leptin isoform X3
XM_021078503.1	XP_020934162.1	leptin isoform X5
NM_213840.1	NP_999005.1	leptin precursor
XM_021078502.1	XP_020934161.1	leptin isoform X4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Effect of heat stress on performance and expression of selected amino acid and glucose transporters, HSP90, leptin and ghrelin in growing pigs.
Cervantes M, Cota M, Arce N, Castillo G, Avelar E, Espinoza S, Morales A
Journal of thermal biology5969-76(2016 Jul)

Haplotypic diversity of porcine LEP and LEPR genes involved in growth and fatness regulation.
P?rez-Montarelo D, Rodr?guez MC, Fern?ndez A, Ben?tez R, Garc?a F, Sili? L, Fern?ndez AI
Journal of applied genetics56(4)525-533(2015 Nov)

Novel polymorphisms in porcine 3'UTR of the leptin gene, including a rare variant within target sequence for MIR-9 gene in Duroc breed, not associated with production traits.
Mankowska M, Szydlowski M, Salamon S, Bartz M, Switonski M
Animal biotechnology26(2)156-63(2015)

Presence of leptin and its receptor in the hypothalamus, uterus and ovaries of Swine females culled with distinct ovarian statuses and parities.
Moreira F, Gheller SM, Mondadori RG, Varela J?nior AS, Corcini CD, Lucia T Jr
Reproduction in domestic animals = Zuchthygiene49(6)1074-8(2014 Dec)

Expression of leptin and its long form receptor at the porcine maternal-fetal interface: contrasting healthy and arresting conceptus attachment sites during early and mid-pregnancy.
Kerr A, Kridli RT, Khalaj K, Wessels JM, Hahnel A, Tayade C
Reproductive biology and endocrinology : RB&E1291(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The lack of effect of heat stress on the expression of leptin suggests that this peptide may not be involved in the reduction of feed intake of HS acclimated pigs.
Title: Effect of heat stress on performance and expression of selected amino acid and glucose transporters, HSP90, leptin and ghrelin in growing pigs.

A high variability of the LEP was detected in the different analysed populations providing new data for the existence of two domestication centres in Asia.
Title: Haplotypic diversity of porcine LEP and LEPR genes involved in growth and fatness regulation.

Studied 3'UTR leptin polymorphism regulatory sequences' affect on gene expression and their association with production traits.
Title: Novel polymorphisms in porcine 3'UTR of the leptin gene, including a rare variant within target sequence for MIR-9 gene in Duroc breed, not associated with production traits.

The presence of leptin and ObR-b varies across parities and is more intense in the uterus, ovaries and hypothalamus of females that were cycling before culling than in those having cystic ovaries.
Title: Presence of leptin and its receptor in the hypothalamus, uterus and ovaries of Swine females culled with distinct ovarian statuses and parities.

Data showing changes in expression patterns of LEP/LEPR in endometrium/chorioallantoic membrane during placentation/fetal development suggest role for LEP/LEPR complex at early stages of pregnancy, possibly affecting the attachment process.
Title: Expression of leptin and its long form receptor at the porcine maternal-fetal interface: contrasting healthy and arresting conceptus attachment sites during early and mid-pregnancy.

The following LEP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe00793	NM_213840.1 Latest version!	Sus scrofa leptin (LEP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OSe00793	XM_021078500.1 Latest version!	Sus scrofa leptin (LEP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OSe241774	XM_021078501.1 Latest version!	Sus scrofa leptin (LEP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OSe241775	XM_021078502.1 Latest version!	Sus scrofa leptin (LEP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OSe241776	XM_021078503.1 Latest version!	Sus scrofa leptin (LEP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OSe241773	XM_021078499.1 Latest version!	Sus scrofa leptin (LEP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LEP ( NM_213840.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999005.1 Sus scrofa leptin (LEP), mRNA.

Price & Availability↑

CloneID	OSe00793
Clone ID Related Accession (Same CDS sequence)	XM_021078500.1 , NM_213840.1
Accession Version	NM_213840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Sus scrofa(pig)
Product	leptin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF052691.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF052691.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3180000, SAMEA3180001 [ECO:0000350] ##Evidence-Data-END##

ATGCGCTGTG GACCCCTGTG CCGATTCCTG TGGCTTTGGC CCTATCTGTC CTACGTTGAA 
GCCGTGCCCA TCTGGAGAGT CCAGGATGAC ACCAAAACCC TCATCAAGAC GATTGTCACC 
AGGATCAGTG ACATTTCACA CATGCAGTCT GTCTCCTCCA AACAGAGGGT CACCGGTTTG 
GACTTCATCC CTGGGCTCCA TCCTGTCCTG AGTTTGTCCA AGATGGACCA GACCCTGGCG 
ATCTACCAAC AGATCCTCAC CAGTCTGCCT TCCAGAAATG TGATCCAAAT ATCGAATGAC 
CTGGAGAACC TCCGGGACCT TCTCCACCTG CTGGCCTCCT CCAAGAGCTG CCCCTTGCCC 
CAGGCCAGGG CCCTGGAGAC CTTGGAGAGC CTGGGCGGCG TCCTGGAAGC CTCCCTCTAC 
TCCACGGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGGCTC TGCAGGACAT GCTGCGGCAG 
CTGGACCTCA GCCCTGGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999005.1
CDS	827..1330
Translation	MRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTRISDISHMQSVSSKQRVTGLDFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLPQARALETLESLGGVLEASLYSTEVVALSRLQGALQDMLRQLDLSPGC

Target ORF information:

RefSeq Version	NM_213840.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa leptin (LEP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213840.1

ORF Insert Sequence:

ATGCGCTGTG GACCCCTGTG CCGATTCCTG TGGCTTTGGC CCTATCTGTC CTACGTTGAA 
GCCGTGCCCA TCTGGAGAGT CCAGGATGAC ACCAAAACCC TCATCAAGAC GATTGTCACC 
AGGATCAGTG ACATTTCACA CATGCAGTCT GTCTCCTCCA AACAGAGGGT CACCGGTTTG 
GACTTCATCC CTGGGCTCCA TCCTGTCCTG AGTTTGTCCA AGATGGACCA GACCCTGGCG 
ATCTACCAAC AGATCCTCAC CAGTCTGCCT TCCAGAAATG TGATCCAAAT ATCGAATGAC 
CTGGAGAACC TCCGGGACCT TCTCCACCTG CTGGCCTCCT CCAAGAGCTG CCCCTTGCCC 
CAGGCCAGGG CCCTGGAGAC CTTGGAGAGC CTGGGCGGCG TCCTGGAAGC CTCCCTCTAC 
TCCACGGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGGCTC TGCAGGACAT GCTGCGGCAG 
CTGGACCTCA GCCCTGGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEP ( XM_021078500.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020934159.1 Sus scrofa leptin (LEP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe00793
Clone ID Related Accession (Same CDS sequence)	XM_021078500.1 , NM_213840.1
Accession Version	XM_021078500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	leptin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010460.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTGTG GACCCCTGTG CCGATTCCTG TGGCTTTGGC CCTATCTGTC CTACGTTGAA 
GCCGTGCCCA TCTGGAGAGT CCAGGATGAC ACCAAAACCC TCATCAAGAC GATTGTCACC 
AGGATCAGTG ACATTTCACA CATGCAGTCT GTCTCCTCCA AACAGAGGGT CACCGGTTTG 
GACTTCATCC CTGGGCTCCA TCCTGTCCTG AGTTTGTCCA AGATGGACCA GACCCTGGCG 
ATCTACCAAC AGATCCTCAC CAGTCTGCCT TCCAGAAATG TGATCCAAAT ATCGAATGAC 
CTGGAGAACC TCCGGGACCT TCTCCACCTG CTGGCCTCCT CCAAGAGCTG CCCCTTGCCC 
CAGGCCAGGG CCCTGGAGAC CTTGGAGAGC CTGGGCGGCG TCCTGGAAGC CTCCCTCTAC 
TCCACGGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGGCTC TGCAGGACAT GCTGCGGCAG 
CTGGACCTCA GCCCTGGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020934159.1
CDS	86..589
Translation	MRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTRISDISHMQSVSSKQRVTGLDFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLPQARALETLESLGGVLEASLYSTEVVALSRLQGALQDMLRQLDLSPGC

Target ORF information:

RefSeq Version	XM_021078500.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa leptin (LEP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078500.1

ORF Insert Sequence:

ATGCGCTGTG GACCCCTGTG CCGATTCCTG TGGCTTTGGC CCTATCTGTC CTACGTTGAA 
GCCGTGCCCA TCTGGAGAGT CCAGGATGAC ACCAAAACCC TCATCAAGAC GATTGTCACC 
AGGATCAGTG ACATTTCACA CATGCAGTCT GTCTCCTCCA AACAGAGGGT CACCGGTTTG 
GACTTCATCC CTGGGCTCCA TCCTGTCCTG AGTTTGTCCA AGATGGACCA GACCCTGGCG 
ATCTACCAAC AGATCCTCAC CAGTCTGCCT TCCAGAAATG TGATCCAAAT ATCGAATGAC 
CTGGAGAACC TCCGGGACCT TCTCCACCTG CTGGCCTCCT CCAAGAGCTG CCCCTTGCCC 
CAGGCCAGGG CCCTGGAGAC CTTGGAGAGC CTGGGCGGCG TCCTGGAAGC CTCCCTCTAC 
TCCACGGAGG TGGTGGCCCT GAGCAGGCTG CAGGGGGCTC TGCAGGACAT GCTGCGGCAG 
CTGGACCTCA GCCCTGGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEP ( XM_021078501.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020934160.1 Sus scrofa leptin (LEP), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe241774
Clone ID Related Accession (Same CDS sequence)	XM_021078501.1
Accession Version	XM_021078501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	leptin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010460.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTGTG GACCCCTGTG CCGATTCCTG TGGCTTTGGC CCTATCTGTC CTACGTTGAA 
GCCGTGCCCA TCTGGAGAGT CCAGGATGAC ACCAAAACCC TCATCAAGAC GATTGTCACC 
AGGATCAGTG ACATTTCACA CATGTCTGTC TCCTCCAAAC AGAGGGTCAC CGGTTTGGAC 
TTCATCCCTG GGCTCCATCC TGTCCTGAGT TTGTCCAAGA TGGACCAGAC CCTGGCGATC 
TACCAACAGA TCCTCACCAG TCTGCCTTCC AGAAATGTGA TCCAAATATC GAATGACCTG 
GAGAACCTCC GGGACCTTCT CCACCTGCTG GCCTCCTCCA AGAGCTGCCC CTTGCCCCAG 
GCCAGGGCCC TGGAGACCTT GGAGAGCCTG GGCGGCGTCC TGGAAGCCTC CCTCTACTCC 
ACGGAGGTGG TGGCCCTGAG CAGGCTGCAG GGGGCTCTGC AGGACATGCT GCGGCAGCTG 
GACCTCAGCC CTGGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020934160.1
CDS	107..607
Translation	MRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTRISDISHMSVSSKQRVTGLDFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLPQARALETLESLGGVLEASLYSTEVVALSRLQGALQDMLRQLDLSPGC

Target ORF information:

RefSeq Version	XM_021078501.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa leptin (LEP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078501.1

ORF Insert Sequence:

ATGCGCTGTG GACCCCTGTG CCGATTCCTG TGGCTTTGGC CCTATCTGTC CTACGTTGAA 
GCCGTGCCCA TCTGGAGAGT CCAGGATGAC ACCAAAACCC TCATCAAGAC GATTGTCACC 
AGGATCAGTG ACATTTCACA CATGTCTGTC TCCTCCAAAC AGAGGGTCAC CGGTTTGGAC 
TTCATCCCTG GGCTCCATCC TGTCCTGAGT TTGTCCAAGA TGGACCAGAC CCTGGCGATC 
TACCAACAGA TCCTCACCAG TCTGCCTTCC AGAAATGTGA TCCAAATATC GAATGACCTG 
GAGAACCTCC GGGACCTTCT CCACCTGCTG GCCTCCTCCA AGAGCTGCCC CTTGCCCCAG 
GCCAGGGCCC TGGAGACCTT GGAGAGCCTG GGCGGCGTCC TGGAAGCCTC CCTCTACTCC 
ACGGAGGTGG TGGCCCTGAG CAGGCTGCAG GGGGCTCTGC AGGACATGCT GCGGCAGCTG 
GACCTCAGCC CTGGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEP ( XM_021078502.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020934161.1 Sus scrofa leptin (LEP), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe241775
Clone ID Related Accession (Same CDS sequence)	XM_021078502.1
Accession Version	XM_021078502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	leptin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010460.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCC GGAGGAATCG AGGAATCGCG GTGCCAGCAG CGGCGAGGCC CCAGAAGCAC 
ATCCCGGAAA GGAAAATGCG CTGTGGACCC CTGTGCCGAT TCCTGTGGCT TTGGCCCTAT 
CTGTCCTACG TTGAAGCCGT GCCCATCTGG AGAGTCCAGG ATGACACCAA AACCCTCATC 
AAGACGATTG TCACCAGGAT CAGTGACATT TCACACATGC AGTCTGTCTC CTCCAAACAG 
AGGGTCACCG GTTTGGACTT CATCCCTGGG CTCCATCCTG TCCTGAGTTT GTCCAAGATG 
GACCAGACCC TGGCGATCTA CCAACAGATC CTCACCAGTC TGCCTTCCAG AAATGTGATC 
CAAATATCGA ATGACCTGGA GAACCTCCGG GACCTTCTCC ACCTGCTGGC CTCCTCCAAG 
AGCTGCCCCT TGCCCCAGGC CAGGGCCCTG GAGACCTTGG AGAGCCTGGG CGGCGTCCTG 
GAAGCCTCCC TCTACTCCAC GGAGGTGGTG GCCCTGAGCA GGCTGCAGGG GGCTCTGCAG 
GACATGCTGC GGCAGCTGGA CCTCAGCCCT GGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020934161.1
CDS	62..640
Translation	MEPRRNRGIAVPAAARPQKHIPERKMRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTRISDISHMQSVSSKQRVTGLDFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLPQARALETLESLGGVLEASLYSTEVVALSRLQGALQDMLRQLDLSPGC

Target ORF information:

RefSeq Version	XM_021078502.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa leptin (LEP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078502.1

ORF Insert Sequence:

ATGGAGCCCC GGAGGAATCG AGGAATCGCG GTGCCAGCAG CGGCGAGGCC CCAGAAGCAC 
ATCCCGGAAA GGAAAATGCG CTGTGGACCC CTGTGCCGAT TCCTGTGGCT TTGGCCCTAT 
CTGTCCTACG TTGAAGCCGT GCCCATCTGG AGAGTCCAGG ATGACACCAA AACCCTCATC 
AAGACGATTG TCACCAGGAT CAGTGACATT TCACACATGC AGTCTGTCTC CTCCAAACAG 
AGGGTCACCG GTTTGGACTT CATCCCTGGG CTCCATCCTG TCCTGAGTTT GTCCAAGATG 
GACCAGACCC TGGCGATCTA CCAACAGATC CTCACCAGTC TGCCTTCCAG AAATGTGATC 
CAAATATCGA ATGACCTGGA GAACCTCCGG GACCTTCTCC ACCTGCTGGC CTCCTCCAAG 
AGCTGCCCCT TGCCCCAGGC CAGGGCCCTG GAGACCTTGG AGAGCCTGGG CGGCGTCCTG 
GAAGCCTCCC TCTACTCCAC GGAGGTGGTG GCCCTGAGCA GGCTGCAGGG GGCTCTGCAG 
GACATGCTGC GGCAGCTGGA CCTCAGCCCT GGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEP ( XM_021078503.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020934162.1 Sus scrofa leptin (LEP), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe241776
Clone ID Related Accession (Same CDS sequence)	XM_021078503.1
Accession Version	XM_021078503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	leptin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010460.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCC GGAGGAATCG AGGAATCGCG GTGCCAGCAG CGGCGAGGCC CCAGAAGCAC 
ATCCCGGAAA GGAAAATGCG CTGTGGACCC CTGTGCCGAT TCCTGTGGCT TTGGCCCTAT 
CTGTCCTACG TTGAAGCCGT GCCCATCTGG AGAGTCCAGG ATGACACCAA AACCCTCATC 
AAGACGATTG TCACCAGGAT CAGTGACATT TCACACATGT CTGTCTCCTC CAAACAGAGG 
GTCACCGGTT TGGACTTCAT CCCTGGGCTC CATCCTGTCC TGAGTTTGTC CAAGATGGAC 
CAGACCCTGG CGATCTACCA ACAGATCCTC ACCAGTCTGC CTTCCAGAAA TGTGATCCAA 
ATATCGAATG ACCTGGAGAA CCTCCGGGAC CTTCTCCACC TGCTGGCCTC CTCCAAGAGC 
TGCCCCTTGC CCCAGGCCAG GGCCCTGGAG ACCTTGGAGA GCCTGGGCGG CGTCCTGGAA 
GCCTCCCTCT ACTCCACGGA GGTGGTGGCC CTGAGCAGGC TGCAGGGGGC TCTGCAGGAC 
ATGCTGCGGC AGCTGGACCT CAGCCCTGGC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020934162.1
CDS	105..680
Translation	MEPRRNRGIAVPAAARPQKHIPERKMRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTIVTRISDISHMSVSSKQRVTGLDFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLPQARALETLESLGGVLEASLYSTEVVALSRLQGALQDMLRQLDLSPGC

Target ORF information:

RefSeq Version	XM_021078503.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa leptin (LEP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078503.1

ORF Insert Sequence:

ATGGAGCCCC GGAGGAATCG AGGAATCGCG GTGCCAGCAG CGGCGAGGCC CCAGAAGCAC 
ATCCCGGAAA GGAAAATGCG CTGTGGACCC CTGTGCCGAT TCCTGTGGCT TTGGCCCTAT 
CTGTCCTACG TTGAAGCCGT GCCCATCTGG AGAGTCCAGG ATGACACCAA AACCCTCATC 
AAGACGATTG TCACCAGGAT CAGTGACATT TCACACATGT CTGTCTCCTC CAAACAGAGG 
GTCACCGGTT TGGACTTCAT CCCTGGGCTC CATCCTGTCC TGAGTTTGTC CAAGATGGAC 
CAGACCCTGG CGATCTACCA ACAGATCCTC ACCAGTCTGC CTTCCAGAAA TGTGATCCAA 
ATATCGAATG ACCTGGAGAA CCTCCGGGAC CTTCTCCACC TGCTGGCCTC CTCCAAGAGC 
TGCCCCTTGC CCCAGGCCAG GGCCCTGGAG ACCTTGGAGA GCCTGGGCGG CGTCCTGGAA 
GCCTCCCTCT ACTCCACGGA GGTGGTGGCC CTGAGCAGGC TGCAGGGGGC TCTGCAGGAC 
ATGCTGCGGC AGCTGGACCT CAGCCCTGGC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEP ( XM_021078499.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020934158.1 Sus scrofa leptin (LEP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe241773
Clone ID Related Accession (Same CDS sequence)	XM_021078499.1
Accession Version	XM_021078499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	leptin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010460.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATGTG ACCGGTGCCT CCAGGACTGT GTGAGCCTGG CTTTCCTCAT CCGCTCCGCA 
CCATCACCCC TGCAGGTGTA TGTTCCCAGG CCAGCCTCAC GGCGTGTCGC TGTGCTTCCC 
CGGCGCCCAG GGCCGTCGGA TCCTACCGGC CAGAGGAATT GGCTGCGCCG GCCTCGCCAT 
CCGCGCACCA CCGGCCAGCG AGGGCGCCGT CTGAGCCCCC AGCAGGCTCT GAGGAACCCC 
CGGCTGCCAC CCTGTCCCCG GCGGCCGTTC CCCGAGGCGC GAGAGGCCGA TCCGGGCGTC 
ACCTCGAGGA TTTCTCATCC CTGCCAGCCG CCCGCAGCAT TTCTGGCGGT TACCCGAGGG 
TGCGCGCGGA GCTCCTGGCG CAGCGGAGTC CCTCCCTCGA GGCCCCGACC AGCTCTGCCC 
GGGCCCAACG CTGCCCCGCC AGGCGGGGCG GGAGCTGGCG CTCGGGGTGG GCCAGGGCCT 
GCCGGGCAGT TGCGCAAGTT GCGCTCGCGC GTCTATAAGA GGGGAGGGCA GGCATGGAGC 
CCCGGAGGAA TCGAGGAATC GCGGTGCCAG CAGCGGCGAG GCCCGATCCC CACCAGCAGC 
GGGATTCCAG TGGTGGCAGC TCCCTGGTGC CCTGCCAGGG AACGCCTTGA AATTCTTCTT 
TGTCCACACA AGTGCCTCAG AGAAGTAGAA AGAGAGGTTG TTTCTACTGA AAAACACCAC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020934158.1
CDS	195..920
Translation	MQCDRCLQDCVSLAFLIRSAPSPLQVYVPRPASRRVAVLPRRPGPSDPTGQRNWLRRPRHPRTTGQRGRRLSPQQALRNPRLPPCPRRPFPEAREADPGVTSRISHPCQPPAAFLAVTRGCARSSWRSGVPPSRPRPALPGPNAAPPGGAGAGARGGPGPAGQLRKLRSRVYKRGGQAWSPGGIEESRCQQRRGPIPTSSGIPVVAAPWCPARERLEILLCPHKCLREVEREVVSTEKHHT

Target ORF information:

RefSeq Version	XM_021078499.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa leptin (LEP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078499.1

ORF Insert Sequence:

ATGCAATGTG ACCGGTGCCT CCAGGACTGT GTGAGCCTGG CTTTCCTCAT CCGCTCCGCA 
CCATCACCCC TGCAGGTGTA TGTTCCCAGG CCAGCCTCAC GGCGTGTCGC TGTGCTTCCC 
CGGCGCCCAG GGCCGTCGGA TCCTACCGGC CAGAGGAATT GGCTGCGCCG GCCTCGCCAT 
CCGCGCACCA CCGGCCAGCG AGGGCGCCGT CTGAGCCCCC AGCAGGCTCT GAGGAACCCC 
CGGCTGCCAC CCTGTCCCCG GCGGCCGTTC CCCGAGGCGC GAGAGGCCGA TCCGGGCGTC 
ACCTCGAGGA TTTCTCATCC CTGCCAGCCG CCCGCAGCAT TTCTGGCGGT TACCCGAGGG 
TGCGCGCGGA GCTCCTGGCG CAGCGGAGTC CCTCCCTCGA GGCCCCGACC AGCTCTGCCC 
GGGCCCAACG CTGCCCCGCC AGGCGGGGCG GGAGCTGGCG CTCGGGGTGG GCCAGGGCCT 
GCCGGGCAGT TGCGCAAGTT GCGCTCGCGC GTCTATAAGA GGGGAGGGCA GGCATGGAGC 
CCCGGAGGAA TCGAGGAATC GCGGTGCCAG CAGCGGCGAG GCCCGATCCC CACCAGCAGC 
GGGATTCCAG TGGTGGCAGC TCCCTGGTGC CCTGCCAGGG AACGCCTTGA AATTCTTCTT 
TGTCCACACA AGTGCCTCAG AGAAGTAGAA AGAGAGGTTG TTTCTACTGA AAAACACCAC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of heat stress on performance and expression of selected amino acid and glucose transporters, HSP90, leptin and ghrelin in growing pigs.
Journal of thermal biology5969-76(2016 Jul)
Cervantes M,Cota M,Arce N,Castillo G,Avelar E,Espinoza S,Morales A

Haplotypic diversity of porcine LEP and LEPR genes involved in growth and fatness regulation.
Journal of applied genetics56(4)525-533(2015 Nov)
P?rez-Montarelo D,Rodr?guez MC,Fern?ndez A,Ben?tez R,Garc?a F,Sili? L,Fern?ndez AI

Novel polymorphisms in porcine 3'UTR of the leptin gene, including a rare variant within target sequence for MIR-9 gene in Duroc breed, not associated with production traits.
Animal biotechnology26(2)156-63(2015)
Mankowska M,Szydlowski M,Salamon S,Bartz M,Switonski M

Presence of leptin and its receptor in the hypothalamus, uterus and ovaries of Swine females culled with distinct ovarian statuses and parities.
Reproduction in domestic animals = Zuchthygiene49(6)1074-8(2014 Dec)
Moreira F,Gheller SM,Mondadori RG,Varela J?nior AS,Corcini CD,Lucia T Jr

Expression of leptin and its long form receptor at the porcine maternal-fetal interface: contrasting healthy and arresting conceptus attachment sites during early and mid-pregnancy.
Reproductive biology and endocrinology : RB&E1291(2014 Sep)
Kerr A,Kridli RT,Khalaj K,Wessels JM,Hahnel A,Tayade C