TNFRSF12A cDNA ORF clone, Sus scrofa(Pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNFRSF12A
Entrez Gene ID	100217390
Full Name	TNF receptor superfamily member 12A
Synonyms	Fn14,TWEAKR
Gene Type	protein-coding
Organism	Sus scrofa(Pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142839.2	NP_001136311.1	tumor necrosis factor receptor superfamily member 12A precursor
XM_013995711.1	XP_013851165.1	tumor necrosis factor receptor superfamily member 12A isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Lipopolysaccharide Upregulated Intestinal Epithelial Cell Expression of Fn14 and Activation of Fn14 Signaling Amplify Intestinal TLR4-Mediated Inflammation.
Qi X, Qin L, Du R, Chen Y, Lei M, Deng M, Wang J
Frontiers in cellular and infection microbiology7315(2017)

Molecular characterization of cytokine TWEAK and its receptor Fn14 in pig (Sus scrofa).
Shui Y, Guan ZB, Zhang SQ
Veterinary immunology and immunopathology126(3-4)396-402(2008 Dec)

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags.
Gorodkin J, Cirera S, Hedegaard J, Gilchrist MJ, Panitz F, J?rgensen C, Scheibye-Knudsen K, Arvin T, Lumholdt S, Sawera M, Green T, Nielsen BJ, Havgaard JH, Rosenkilde C, Wang J, Li H, Li R, Liu B, Hu S, Dong W, Li W, Yu J, Wang J, Staefeldt HH, Wernersson R, Madsen LB, Thomsen B, Hornsh?j H, Bujie Z, Wang X, Wang X, Bolund L, Brunak S, Yang H, Bendixen C, Fredholm M
Genome biology8(4)R45(2007)

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
J?rgensen FG, Hobolth A, Hornsh?j H, Bendixen C, Fredholm M, Schierup MH
BMC biology32(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TNFRSF12A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNFRSF12A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe06921	NM_001142839.2 Latest version!	Sus scrofa TNF receptor superfamily member 12A (TNFRSF12A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OSe33845	XM_013995711.1 Latest version!	Sus scrofa tumor necrosis factor receptor superfamily, member 12A (TNFRSF12A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNFRSF12A ( NM_001142839.2 ) cDNA ORF clone, Sus scrofa(Pig) -> NP_001136311.1 Sus scrofa TNF receptor superfamily member 12A (TNFRSF12A), mRNA.

Price & Availability↑

CloneID	OSe06921
Clone ID Related Accession (Same CDS sequence)	NM_001142839.2
Accession Version	NM_001142839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-27
Organism	Sus scrofa(pig)
Product	tumor necrosis factor receptor superfamily member 12A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AEMK02000018.1 and EW508106.2. On or before Jan 27, 2017 this sequence version replaced XM_005662193.2, NM_001142839.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5275317.318321.1, SRR5275317.130193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04484924, SAMN04484941 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTCTCA CCCCGCTGCG CCCGCTGCTG CGGCTCCTCG TGCTGGGGCT CGGGCTGGTG 
CTGCTGCGCG CCACGGCCGG AGAGCGAGTG CCAGGCACCA CCCCCTGCTC TCGCGGCAGC 
TCCTGGAGCG CGGACCTAGA CAAGTGCATG GACTGTGCGT CGTGCCCCGC GCGACCGCAC 
AGCGACTTCT GCCTGGGCTG CGCCGCGGCC CCTCCGGCCT CTTTCAGGCT GCTGTGGCCC 
ATTCTGGGGG GCGCCCTGAG CCTGGTCCTC GTGCTGGGGC TGCTTTCTGG CTTCCTGGTC 
TGGAGACGGT GCCGCAGGAG AGAGAAGTTT ACCACTCCCA TCGAGGAGAC CGGCGGGGAG 
GGCTGCCCTG GCGTGGCGCT GATCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136311.1
CDS	22..411
Translation	MILTPLRPLLRLLVLGLGLVLLRATAGERVPGTTPCSRGSSWSADLDKCMDCASCPARPHSDFCLGCAAAPPASFRLLWPILGGALSLVLVLGLLSGFLVWRRCRRREKFTTPIEETGGEGCPGVALIQ

Target ORF information:

RefSeq Version	NM_001142839.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa TNF receptor superfamily member 12A (TNFRSF12A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142839.2

ORF Insert Sequence:

ATGATTCTCA CCCCGCTGCG CCCGCTGCTG CGGCTCCTCG TGCTGGGGCT CGGGCTGGTG 
CTGCTGCGCG CCACGGCCGG AGAGCGAGTG CCAGGCACCA CCCCCTGCTC TCGCGGCAGC 
TCCTGGAGCG CGGACCTAGA CAAGTGCATG GACTGTGCGT CGTGCCCCGC GCGACCGCAC 
AGCGACTTCT GCCTGGGCTG CGCCGCGGCC CCTCCGGCCT CTTTCAGGCT GCTGTGGCCC 
ATTCTGGGGG GCGCCCTGAG CCTGGTCCTC GTGCTGGGGC TGCTTTCTGG CTTCCTGGTC 
TGGAGACGGT GCCGCAGGAG AGAGAAGTTT ACCACTCCCA TCGAGGAGAC CGGCGGGGAG 
GGCTGCCCTG GCGTGGCGCT GATCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNFRSF12A ( XM_013995711.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_013851165.1 Sus scrofa tumor necrosis factor receptor superfamily, member 12A (TNFRSF12A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe33845
Clone ID Related Accession (Same CDS sequence)	XM_013995711.1
Accession Version	XM_013995711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	tumor necrosis factor receptor superfamily member 12A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609807.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA CTGCGTCTTT CCGGCGTTCT GGACGCTCCC GTCCGGAGAC AGTGTCAGTT 
GAGCGACTGA CTGAACTTAA GGAGCTGGGC GGGACCGGGT GTCCAGGGTT GGATCGTGGG 
GCGGGTAGAG GCGAGGGATC CGCCCCGGAA GGCGGGGGCG GGGCCGGGGC CGTCTCTCCC 
GGTCCCCCCG CGGCGGGCGC AAACGGCGGC GGTCGCGGGA CAGGCACCAT GATTCTCACC 
CCGCTGCGCC CGCTGCTGCG GCTCCTCGTG CTGGGGCTCG GGCTGGTGCT GCTGCGCGCC 
ACGGCCGGAG AGCGAGTGCC AGGCGCCGCG GCCCCTCCGG CCTCTTTCAG GCTGCTGTGG 
CCCATTCTGG GGGGCGCCCT GAGCCTGGTC CTCGTGCTGG GGCTGCTTTC TGGCTTCCTG 
GTCTGGAGAC GGTGCCGCAG GAGAGAGAAG TTTACCACTC CCATCGAGGA GACCGGCGGG 
GAGGGCTGCC CTGGCGTGGC GCTGATCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013851165.1
CDS	56..568
Translation	MARTASFRRSGRSRPETVSVERLTELKELGGTGCPGLDRGAGRGEGSAPEGGGGAGAVSPGPPAAGANGGGRGTGTMILTPLRPLLRLLVLGLGLVLLRATAGERVPGAAAPPASFRLLWPILGGALSLVLVLGLLSGFLVWRRCRRREKFTTPIEETGGEGCPGVALIQ

Target ORF information:

RefSeq Version	XM_013995711.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa tumor necrosis factor receptor superfamily, member 12A (TNFRSF12A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013995711.1

ORF Insert Sequence:

ATGGCCAGAA CTGCGTCTTT CCGGCGTTCT GGACGCTCCC GTCCGGAGAC AGTGTCAGTT 
GAGCGACTGA CTGAACTTAA GGAGCTGGGC GGGACCGGGT GTCCAGGGTT GGATCGTGGG 
GCGGGTAGAG GCGAGGGATC CGCCCCGGAA GGCGGGGGCG GGGCCGGGGC CGTCTCTCCC 
GGTCCCCCCG CGGCGGGCGC AAACGGCGGC GGTCGCGGGA CAGGCACCAT GATTCTCACC 
CCGCTGCGCC CGCTGCTGCG GCTCCTCGTG CTGGGGCTCG GGCTGGTGCT GCTGCGCGCC 
ACGGCCGGAG AGCGAGTGCC AGGCGCCGCG GCCCCTCCGG CCTCTTTCAG GCTGCTGTGG 
CCCATTCTGG GGGGCGCCCT GAGCCTGGTC CTCGTGCTGG GGCTGCTTTC TGGCTTCCTG 
GTCTGGAGAC GGTGCCGCAG GAGAGAGAAG TTTACCACTC CCATCGAGGA GACCGGCGGG 
GAGGGCTGCC CTGGCGTGGC GCTGATCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lipopolysaccharide Upregulated Intestinal Epithelial Cell Expression of Fn14 and Activation of Fn14 Signaling Amplify Intestinal TLR4-Mediated Inflammation.
Frontiers in cellular and infection microbiology7315(2017)
Qi X,Qin L,Du R,Chen Y,Lei M,Deng M,Wang J

Molecular characterization of cytokine TWEAK and its receptor Fn14 in pig (Sus scrofa).
Veterinary immunology and immunopathology126(3-4)396-402(2008 Dec)
Shui Y,Guan ZB,Zhang SQ

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags.
Genome biology8(4)R45(2007)
Gorodkin J,Cirera S,Hedegaard J,Gilchrist MJ,Panitz F,J?rgensen C,Scheibye-Knudsen K,Arvin T,Lumholdt S,Sawera M,Green T,Nielsen BJ,Havgaard JH,Rosenkilde C,Wang J,Li H,Li R,Liu B,Hu S,Dong W,Li W,Yu J,Wang J,Staefeldt HH,Wernersson R,Madsen LB,Thomsen B,Hornsh?j H,Bujie Z,Wang X,Wang X,Bolund L,Brunak S,Yang H,Bendixen C,Fredholm M

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
BMC biology32(2005 Jan)
J?rgensen FG,Hobolth A,Hornsh?j H,Bendixen C,Fredholm M,Schierup MH