ARFGEF1 cDNA ORF clone, Sturnus vulgaris(Common starling)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARFGEF1
Entrez Gene ID	106851228
Full Name	ADP ribosylation factor guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Sturnus vulgaris(Common starling)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014873010.1	XP_014728496.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
XM_014873011.1	XP_014728497.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following ARFGEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARFGEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt06344	XM_014873010.1 Latest version!	Sturnus vulgaris ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSt06345	XM_014873011.1 Latest version!	Sturnus vulgaris ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARFGEF1 ( XM_014873010.1 ) cDNA ORF clone, Sturnus vulgaris(Common starling) -> XP_014728496.1 Sturnus vulgaris ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.

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CloneID	OSt06344
Clone ID Related Accession (Same CDS sequence)	XM_014873010.1
Accession Version	XM_014873010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-01
Organism	Sturnus vulgaris(Common starling)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014650511.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sturnus vulgaris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GGAAGAAGAC GAAAAACATG TTTCTGACCC GGGCCCTGGA GAAGATCCTG 
GCCGATAAGG AGGTGAAGAA GGCGCATCAC TCCCAACTGC GCAAAGCCTG CGAGGTGGCA 
CTAGAGGAAA TAAAGGCTGA AACAGAAAAA CAAAGTCCCC CTCATGGAGA AGCAAAGTCT 
GGTTCAAGCA CTCTTCCACC TGTGAAATCA AAGACCAGCT TCATTGAAGC AGACAAATAC 
TTCCTACCAT TTGAATTGGC ATGCCAGTCC AAGTGCCCCA GGATTGTCAG TACATCTCTA 
GATTGTTTAC AGAAACTTAT AGCATATGGG CACTTGACAG GGAATGCTCC AGACAGTACA 
GCTCCAGGAA AAAAACTAAT TGATAGAATT ATCGAGACAA TATGTGGCTG TTTTCAAGGT 
CCTCAAACAG ACGAAGGGGT TCAGCTACAA ATAATAAAGG CTTTGCTCAC TGCAGTAACA 
TCTCAACATA TAGAAATCCA CGAAGGAACA GTACTACAGG CTGTAAGAAC ATGTTACAAT 
ATCTACCTAG CCAGCAAAAA TCTTATCAAT CAAACTACAG CCAAGGCCAC TCTAACACAA 
ATGCTGAATG TCATTTTTGC ACGTATGGAA AATCAAGCAC TTCAGGAAGC CAAACAGATG 
GAAAAGGAAA GGCACAGACA GCAACACCAT CTCCAGCAGT CTCCAGTAAG CCATCATGAG 
CCTGAATCAC CTCAGTTGAG ACATATTCCA GCACAGGCTG ATCAGCTGTC CAAAGACCAT 
GAGAGAGAGC TTGACCACAG CACAAACGAT ATGGACAAGA ACCTTCAAGA AGATATTGAG 
GCAGAAAATG GATCTGACAT TTCTAGTGCT GAAAATGAAC AGACAGAAGC TGACCAAGCC 
ACAGCAGCAG AAAATCTTTC TAAAGCTGAT GGAGGAACAT ACGATGGTGA AAGCAATGAG 
TGTGAAGAGA AGGCACAAGA AATTGTAGAA AGTATTGTGC AGGAAATGGT GAACATAGTA 
GTTGGAGATG TGGAAGGGAC TGCAAGTGGT GATGGCACAA CTGTATCATT AGAGGATGGC 
AGTGACAGTG AAAACATTCA GGCAAATGGA ATTCCAGGGA CTCCTATTTC TGTTGCTTAT 
ACACCATCTT TACCCGATGA CAGATTATCT GTCTCTTCCA ACGATACTCA GGAATCTGGA 
AATTCTTCAG GACCTACCCC TGGTGCTAAG TTTTCCCACA TTTTACAAAA GGATGCCTTC 
CTTGTATTCA GGTCACTGTG TAAACTCTCT ATGAAGCCCC TGTCTGATGG ACCACCAGAT 
CCAAAATCTC ATGAACTGCG ATCAAAGATT CTTTCATTGC AGTTGCTTCT GTCCATTCTG 
CAAAATGCAG GACCAGTCTT CAGGACAAAT GAGATGTTCA TTAATGCTAT AAAGCAGTAC 
CTCTGTGTTG CACTGTCAAA AAATGGAGTC TCATCAGTTC CAGAAGTTTT TGAACTTTCA 
CTTTCCATAT TTCTTACCCT TCTGTCAAAC TTCAAGACTC ATCTAAAGAT GCAGATTGAG 
GTTTTCTTCA AAGAAATTTT CCTGTATATC TTGGAAACTT CTACTAGCTC ATTTGATCAT 
AAGTGGATGG TTATACAAAC GCTGACAAGG ATATGTGCAG ATGCTCAGAG CGTGGTGGAC 
ATCTACGTGA ACTATGATTG TGATTTAAAT GCAGCAAATA TATTTGAAAG GCTGGTTAAT 
GACTTGTCAA AGATTGCTCA AGGAAGGGGT AGCCAAGAAC TTGGCATGTC CAATGTTCAG 
GAATTAAGTT TGAGGAAAAA AGGATTAGAA TGCTTAGTAT CAATCTTGAA ATGCATGGTG 
GAGTGGAGTA AGGATCAATA TGTAAATCCC AATTCCCAGA CAACCCTTGG CCAAGAAAAA 
CCCACAGAAC AGGACAGCAG TGAAACCAAA CACCCTGAGA CAATTAACAG ATATGGAAGC 
TTAAATTCTT TGGATTCTAC TGCTTCATCA GGAATTGGCA GTTACAGCAC ACAGATGTCT 
GGCACTGACA ACCCAGAGCA GTTTGAAGTC CTAAAGCAGC AAAAGGAAAT AATAGAACAA 
GGAATTGACT TATTCAACAA GAAACCAAAG AGGGGGATTC AGTACCTACA AGAACAAGGA 
ATGCTTGGTA CTACCCCTGA AGATATTGCT CAATTCTTGC ATCAAGAGGA AAGATTAGAT 
TCAACTCAGG TTGGGGAGTT CCTGGGAGAC AACGATAAAT TTAACAAAGA AGTCATGTAT 
GCATATGTAG ACCAGCACGA CTTTTCGGGA AAGGATTTTG TTTCAGCTCT GCGCATGTTT 
CTAGAAGGAT TTCGTCTGCC AGGAGAAGCT CAAAAAATTG ATCGCTTAAT GGAGAAATTT 
GCTGCTAGAT ATTTAGAATG TAACCAAGGG CAAACTCTTT TTGCTAGTGC AGATACTGCA 
TATGTTTTAG CTTACTCAAT TATCATGTTG ACAACAGATC TTCACAGTCC ACAGGTTAAG 
AATAAAATGA CAAAAGAACA GTATATTAAA ATGAATAGAG GTATCAATGA CAGTAAAGAT 
CTCCCTGAAG AATATTTGTC TGCTATCTAT AATGAAATAG CTGGAAAGAA GATTTCAATG 
AAAGAAACAA AAGAATTAAC AATACCCACA AAGTCCAGTA AACAAAGTGT TGCTAGTGAG 
AAGCAGAGAA GACTTCTATA TAACTTGGAA ATGGAACAAA TGGCTAAAAC AGCTAAAGCT 
CTTATGGAGG CAGTGAGCCA CGTTCAGGCC CCTTTTACTA GTGCAACACA CCTGGAGCAT 
GTGAGACCCA TGTTTAAGTT GGCATGGACA CCTTTCCTGG CTGCATTCAG TGTGGGTCTG 
CAGGACTGTG ATGACACCGA AGTTGCCTCT CTTTGTTTGG AGGGTATACG ATGTGCAATC 
CGGATTGCTT GCATTTTCAA CATTCAGGTA AAAAATCTTG AAAGAGATGC CTATGTTCAA 
GCATTAGCAA GATTTACATT GCTTACAGTG AGTTCTGGTA TTACTGAAAT GAAGCAGAAG 
AATATTGACA CCATTAAAAC TCTCATCACG GTGGCTCATA CAGATGGAAA CTACTTAGGA 
AATTCCTGGC ATGAGATTCT GAAATGCATC AGTCAGCTTG AACTGGCACA GTTAATAGGA 
ACTGGAGTAA AACCTCGCTA CATCTCAGGG ACAGTTCGTG GCAGGGAAGG TTCTTTTACT 
GGAACAAAAG ATCAGGCACC TGATGAATTT GTGGGGCTGG GACTGGTTGG TGGAAACGTG 
GATTGGAAGC AGATAGCAAG TATTCAGGAA TCTATTGGTG AAACTAGCTC CCAAAGCGTT 
GTTGTTGCTG TAGACAGAAT ATTTACGGGA TCTACAAGGT TGGATGGAAA TGCTATTGTG 
GATTTTGTTC GCTGGCTTTG TGCTGTGTCT ATGGACGAGC TGCTGTCTGC TACCCACCCT 
CGAATGTTTA GTCTGCAGAA GATTGTAGAG ATTTCTTATT ACAACATGGG CCGGATAAGG 
TTACAGTGGT CTAGGATCTG GGAAGTTATT GGCGATCATT TTAATAAGGT TGGCTGCAAT 
CCCAATGAAG ATGTAGCTAT TTTTGCAGTG GACTCATTAA GGCAATTGTC AATGAAGTTC 
TTGGAAAAAG GAGAACTTGC TAATTTCCGA TTCCAGAAGG ACTTCCTAAG ACCATTTGAA 
CATATCATGA AGAGAAACAG GTCTCCTACT ATTCGAGACA TGGTTGTGCG GTGTATTGCG 
CAGATGGTGA ATTCCCAGGC TGCTAACATT CGTTCTGGTT GGAAAAACAT TTTTTCAGTG 
TTTCATCTGG CAGCCTCAGA TCAGGATGAA AGTATAGTGG AACTGGCATT CCAGACTACA 
GGACACATTG TCACAATCGT GTTTGAAAAG CACTTCCCAG CGACCATAGA TTCTTTCCAG 
GATGCAGTGA AGTGCTTGTC TGAATTTGCC TGCAATGCAG CATTTCCAGA TACCAGTATG 
GAAGCGATCC GCCTCATTCG CCACTGTGCA AAATATGTGT CTGACAGGCC TCAGGCATTC 
AAGGAATACA CAAGTGATGA TATGAATGTA GCTCCTGAAG ACAGAGTGTG GGTGCGAGGA 
TGGTTCCCAA TTCTGTTTGA GTTGTCCTGT ATCATCAATA GATGCAAATT AGATGTAAGA 
ACCAGAGGCT TAACAGTAAT GTTTGAAATA ATGAAGACAT ATGGCCATAC TTATGAAAAA 
CACTGGTGGC AAGATTTGTT TCGGATTGTC TTCAGGATAT TTGACAACAT GAAACTGCCA 
GAACAGCAGA CTGAGAAAGC CGAATGGATG ACAACTACCT GTAACCATGC ACTTTATGCA 
ATATGTGATG TATTTACACA GTATTTAGAA GTACTTAGTG ATGTTCTTTT GGATGATATA 
TTTGCACAGC TGTACTGGTG TGTGCAGCAA GACAATGAAC AACTGGCACG ATCTGGTACA 
AACTGTTTGG AGAATGTTGT CATCCTTAAT GGTGAAAAGT TCACCCTAGA AATCTGGGAT 
AAAACTTGCA CTTGCATGCT GGATATTTTC AAAACTACAA TCCCTCATGC GCTGCTGACT 
TGGCGACCAG TCGGTGGGGA GTTCTGCTCT GGCTCTCCCT CTGATGCAAA AGAAAAACTG 
GATACAATCT CGCAGAAGTC AGTAGATATC CATGATTCCA TCCAGCCTCG ATCTTCAGAT 
GGGCGCCCGT ATCAACCCAG CACAGGGCCC ACGGTAGCTG AAGAAACGAG TAAAGCCAGG 
CCAGCAGCAA AATTCCCAGA ACAGAAGTTA TTTGCTGCTC TTTTGATCAA GTGTGTTGTA 
CAGCTGGAAC TCATTCAGAC GATCGACAAC ATTGTGTTCT TCCCAGCAAC TAGCAAAAAA 
GAGGATGCAG AGAATCTAGC AGCAGCACAA AGAGATGCAG TAGACTTCGA TGTCCATGTG 
GATACACAGG ATCAAGGGAT GTATCGTTTC CTAACATCAC AGCAGCTTTT CAAACTTCTC 
GATTGTCTGC TGGAGTCTCA CAGATTTGCT AAAGCATTTA ATTCAAACAA TGAACAGAGA 
ACTGCTCTCT GGAAAGCAGG TTTCAAAGGC AAATCAAAGC CCAACCTGCT GAAGCAGGAG 
ACGAGCAGCC TGGCCTGTGG GCTGCGCATC CTGTTCCGCA TGTACATGGA CGAGAGCCGC 
ACCAGCGCCT GGGAGGAAGT CCAGCAAAGG CTGCTGAATG TCTGCAGCGA GGCTCTGAGC 
TATTTCCTCA CTCTTACATC AGAAAGTCAC CGGGAAGCCT GGACTAACCT TTTACTCTTA 
TTTTTGACTA AAGTCCTGAA GATAAGTGAT GAAAGGTTCA AGGCTCATGC CTCATTCTAC 
TATCCTTTGT TGTGTGAAAT CATGCAGTTT GACCTTATTC CTGAGCTTCG AGCTGTTTTG 
AGAAGGTTTT TCCTGCGGAT TGGTGTGGTT TTCCAAATAT CCCAACCACC AGAACAAGAG 
TGTGGAATAA GCAAGCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014728496.1
CDS	41..5581
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKSGSSTLPPVKSKTSFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTAPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQQHHLQQSPVSHHEPESPQLRHIPAQADQLSKDHERELDHSTNDMDKNLQEDIEAENGSDISSAENEQTEADQATAAENLSKADGGTYDGESNECEEKAQEIVESIVQEMVNIVVGDVEGTASGDGTTVSLEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPTPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPTEQDSSETKHPETINRYGSLNSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFNIQVKNLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSFTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSATHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTIVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCTCMLDIFKTTIPHALLTWRPVGGEFCSGSPSDAKEKLDTISQKSVDIHDSIQPRSSDGRPYQPSTGPTVAEETSKARPAAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVHVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRTSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDERFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQECGISKH

Target ORF information:

RefSeq Version	XM_014873010.1
Organism	Sturnus vulgaris(Common starling)
Definition	Sturnus vulgaris ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014873010.1

ORF Insert Sequence:

ATGTATGAAG GGAAGAAGAC GAAAAACATG TTTCTGACCC GGGCCCTGGA GAAGATCCTG 
GCCGATAAGG AGGTGAAGAA GGCGCATCAC TCCCAACTGC GCAAAGCCTG CGAGGTGGCA 
CTAGAGGAAA TAAAGGCTGA AACAGAAAAA CAAAGTCCCC CTCATGGAGA AGCAAAGTCT 
GGTTCAAGCA CTCTTCCACC TGTGAAATCA AAGACCAGCT TCATTGAAGC AGACAAATAC 
TTCCTACCAT TTGAATTGGC ATGCCAGTCC AAGTGCCCCA GGATTGTCAG TACATCTCTA 
GATTGTTTAC AGAAACTTAT AGCATATGGG CACTTGACAG GGAATGCTCC AGACAGTACA 
GCTCCAGGAA AAAAACTAAT TGATAGAATT ATCGAGACAA TATGTGGCTG TTTTCAAGGT 
CCTCAAACAG ACGAAGGGGT TCAGCTACAA ATAATAAAGG CTTTGCTCAC TGCAGTAACA 
TCTCAACATA TAGAAATCCA CGAAGGAACA GTACTACAGG CTGTAAGAAC ATGTTACAAT 
ATCTACCTAG CCAGCAAAAA TCTTATCAAT CAAACTACAG CCAAGGCCAC TCTAACACAA 
ATGCTGAATG TCATTTTTGC ACGTATGGAA AATCAAGCAC TTCAGGAAGC CAAACAGATG 
GAAAAGGAAA GGCACAGACA GCAACACCAT CTCCAGCAGT CTCCAGTAAG CCATCATGAG 
CCTGAATCAC CTCAGTTGAG ACATATTCCA GCACAGGCTG ATCAGCTGTC CAAAGACCAT 
GAGAGAGAGC TTGACCACAG CACAAACGAT ATGGACAAGA ACCTTCAAGA AGATATTGAG 
GCAGAAAATG GATCTGACAT TTCTAGTGCT GAAAATGAAC AGACAGAAGC TGACCAAGCC 
ACAGCAGCAG AAAATCTTTC TAAAGCTGAT GGAGGAACAT ACGATGGTGA AAGCAATGAG 
TGTGAAGAGA AGGCACAAGA AATTGTAGAA AGTATTGTGC AGGAAATGGT GAACATAGTA 
GTTGGAGATG TGGAAGGGAC TGCAAGTGGT GATGGCACAA CTGTATCATT AGAGGATGGC 
AGTGACAGTG AAAACATTCA GGCAAATGGA ATTCCAGGGA CTCCTATTTC TGTTGCTTAT 
ACACCATCTT TACCCGATGA CAGATTATCT GTCTCTTCCA ACGATACTCA GGAATCTGGA 
AATTCTTCAG GACCTACCCC TGGTGCTAAG TTTTCCCACA TTTTACAAAA GGATGCCTTC 
CTTGTATTCA GGTCACTGTG TAAACTCTCT ATGAAGCCCC TGTCTGATGG ACCACCAGAT 
CCAAAATCTC ATGAACTGCG ATCAAAGATT CTTTCATTGC AGTTGCTTCT GTCCATTCTG 
CAAAATGCAG GACCAGTCTT CAGGACAAAT GAGATGTTCA TTAATGCTAT AAAGCAGTAC 
CTCTGTGTTG CACTGTCAAA AAATGGAGTC TCATCAGTTC CAGAAGTTTT TGAACTTTCA 
CTTTCCATAT TTCTTACCCT TCTGTCAAAC TTCAAGACTC ATCTAAAGAT GCAGATTGAG 
GTTTTCTTCA AAGAAATTTT CCTGTATATC TTGGAAACTT CTACTAGCTC ATTTGATCAT 
AAGTGGATGG TTATACAAAC GCTGACAAGG ATATGTGCAG ATGCTCAGAG CGTGGTGGAC 
ATCTACGTGA ACTATGATTG TGATTTAAAT GCAGCAAATA TATTTGAAAG GCTGGTTAAT 
GACTTGTCAA AGATTGCTCA AGGAAGGGGT AGCCAAGAAC TTGGCATGTC CAATGTTCAG 
GAATTAAGTT TGAGGAAAAA AGGATTAGAA TGCTTAGTAT CAATCTTGAA ATGCATGGTG 
GAGTGGAGTA AGGATCAATA TGTAAATCCC AATTCCCAGA CAACCCTTGG CCAAGAAAAA 
CCCACAGAAC AGGACAGCAG TGAAACCAAA CACCCTGAGA CAATTAACAG ATATGGAAGC 
TTAAATTCTT TGGATTCTAC TGCTTCATCA GGAATTGGCA GTTACAGCAC ACAGATGTCT 
GGCACTGACA ACCCAGAGCA GTTTGAAGTC CTAAAGCAGC AAAAGGAAAT AATAGAACAA 
GGAATTGACT TATTCAACAA GAAACCAAAG AGGGGGATTC AGTACCTACA AGAACAAGGA 
ATGCTTGGTA CTACCCCTGA AGATATTGCT CAATTCTTGC ATCAAGAGGA AAGATTAGAT 
TCAACTCAGG TTGGGGAGTT CCTGGGAGAC AACGATAAAT TTAACAAAGA AGTCATGTAT 
GCATATGTAG ACCAGCACGA CTTTTCGGGA AAGGATTTTG TTTCAGCTCT GCGCATGTTT 
CTAGAAGGAT TTCGTCTGCC AGGAGAAGCT CAAAAAATTG ATCGCTTAAT GGAGAAATTT 
GCTGCTAGAT ATTTAGAATG TAACCAAGGG CAAACTCTTT TTGCTAGTGC AGATACTGCA 
TATGTTTTAG CTTACTCAAT TATCATGTTG ACAACAGATC TTCACAGTCC ACAGGTTAAG 
AATAAAATGA CAAAAGAACA GTATATTAAA ATGAATAGAG GTATCAATGA CAGTAAAGAT 
CTCCCTGAAG AATATTTGTC TGCTATCTAT AATGAAATAG CTGGAAAGAA GATTTCAATG 
AAAGAAACAA AAGAATTAAC AATACCCACA AAGTCCAGTA AACAAAGTGT TGCTAGTGAG 
AAGCAGAGAA GACTTCTATA TAACTTGGAA ATGGAACAAA TGGCTAAAAC AGCTAAAGCT 
CTTATGGAGG CAGTGAGCCA CGTTCAGGCC CCTTTTACTA GTGCAACACA CCTGGAGCAT 
GTGAGACCCA TGTTTAAGTT GGCATGGACA CCTTTCCTGG CTGCATTCAG TGTGGGTCTG 
CAGGACTGTG ATGACACCGA AGTTGCCTCT CTTTGTTTGG AGGGTATACG ATGTGCAATC 
CGGATTGCTT GCATTTTCAA CATTCAGGTA AAAAATCTTG AAAGAGATGC CTATGTTCAA 
GCATTAGCAA GATTTACATT GCTTACAGTG AGTTCTGGTA TTACTGAAAT GAAGCAGAAG 
AATATTGACA CCATTAAAAC TCTCATCACG GTGGCTCATA CAGATGGAAA CTACTTAGGA 
AATTCCTGGC ATGAGATTCT GAAATGCATC AGTCAGCTTG AACTGGCACA GTTAATAGGA 
ACTGGAGTAA AACCTCGCTA CATCTCAGGG ACAGTTCGTG GCAGGGAAGG TTCTTTTACT 
GGAACAAAAG ATCAGGCACC TGATGAATTT GTGGGGCTGG GACTGGTTGG TGGAAACGTG 
GATTGGAAGC AGATAGCAAG TATTCAGGAA TCTATTGGTG AAACTAGCTC CCAAAGCGTT 
GTTGTTGCTG TAGACAGAAT ATTTACGGGA TCTACAAGGT TGGATGGAAA TGCTATTGTG 
GATTTTGTTC GCTGGCTTTG TGCTGTGTCT ATGGACGAGC TGCTGTCTGC TACCCACCCT 
CGAATGTTTA GTCTGCAGAA GATTGTAGAG ATTTCTTATT ACAACATGGG CCGGATAAGG 
TTACAGTGGT CTAGGATCTG GGAAGTTATT GGCGATCATT TTAATAAGGT TGGCTGCAAT 
CCCAATGAAG ATGTAGCTAT TTTTGCAGTG GACTCATTAA GGCAATTGTC AATGAAGTTC 
TTGGAAAAAG GAGAACTTGC TAATTTCCGA TTCCAGAAGG ACTTCCTAAG ACCATTTGAA 
CATATCATGA AGAGAAACAG GTCTCCTACT ATTCGAGACA TGGTTGTGCG GTGTATTGCG 
CAGATGGTGA ATTCCCAGGC TGCTAACATT CGTTCTGGTT GGAAAAACAT TTTTTCAGTG 
TTTCATCTGG CAGCCTCAGA TCAGGATGAA AGTATAGTGG AACTGGCATT CCAGACTACA 
GGACACATTG TCACAATCGT GTTTGAAAAG CACTTCCCAG CGACCATAGA TTCTTTCCAG 
GATGCAGTGA AGTGCTTGTC TGAATTTGCC TGCAATGCAG CATTTCCAGA TACCAGTATG 
GAAGCGATCC GCCTCATTCG CCACTGTGCA AAATATGTGT CTGACAGGCC TCAGGCATTC 
AAGGAATACA CAAGTGATGA TATGAATGTA GCTCCTGAAG ACAGAGTGTG GGTGCGAGGA 
TGGTTCCCAA TTCTGTTTGA GTTGTCCTGT ATCATCAATA GATGCAAATT AGATGTAAGA 
ACCAGAGGCT TAACAGTAAT GTTTGAAATA ATGAAGACAT ATGGCCATAC TTATGAAAAA 
CACTGGTGGC AAGATTTGTT TCGGATTGTC TTCAGGATAT TTGACAACAT GAAACTGCCA 
GAACAGCAGA CTGAGAAAGC CGAATGGATG ACAACTACCT GTAACCATGC ACTTTATGCA 
ATATGTGATG TATTTACACA GTATTTAGAA GTACTTAGTG ATGTTCTTTT GGATGATATA 
TTTGCACAGC TGTACTGGTG TGTGCAGCAA GACAATGAAC AACTGGCACG ATCTGGTACA 
AACTGTTTGG AGAATGTTGT CATCCTTAAT GGTGAAAAGT TCACCCTAGA AATCTGGGAT 
AAAACTTGCA CTTGCATGCT GGATATTTTC AAAACTACAA TCCCTCATGC GCTGCTGACT 
TGGCGACCAG TCGGTGGGGA GTTCTGCTCT GGCTCTCCCT CTGATGCAAA AGAAAAACTG 
GATACAATCT CGCAGAAGTC AGTAGATATC CATGATTCCA TCCAGCCTCG ATCTTCAGAT 
GGGCGCCCGT ATCAACCCAG CACAGGGCCC ACGGTAGCTG AAGAAACGAG TAAAGCCAGG 
CCAGCAGCAA AATTCCCAGA ACAGAAGTTA TTTGCTGCTC TTTTGATCAA GTGTGTTGTA 
CAGCTGGAAC TCATTCAGAC GATCGACAAC ATTGTGTTCT TCCCAGCAAC TAGCAAAAAA 
GAGGATGCAG AGAATCTAGC AGCAGCACAA AGAGATGCAG TAGACTTCGA TGTCCATGTG 
GATACACAGG ATCAAGGGAT GTATCGTTTC CTAACATCAC AGCAGCTTTT CAAACTTCTC 
GATTGTCTGC TGGAGTCTCA CAGATTTGCT AAAGCATTTA ATTCAAACAA TGAACAGAGA 
ACTGCTCTCT GGAAAGCAGG TTTCAAAGGC AAATCAAAGC CCAACCTGCT GAAGCAGGAG 
ACGAGCAGCC TGGCCTGTGG GCTGCGCATC CTGTTCCGCA TGTACATGGA CGAGAGCCGC 
ACCAGCGCCT GGGAGGAAGT CCAGCAAAGG CTGCTGAATG TCTGCAGCGA GGCTCTGAGC 
TATTTCCTCA CTCTTACATC AGAAAGTCAC CGGGAAGCCT GGACTAACCT TTTACTCTTA 
TTTTTGACTA AAGTCCTGAA GATAAGTGAT GAAAGGTTCA AGGCTCATGC CTCATTCTAC 
TATCCTTTGT TGTGTGAAAT CATGCAGTTT GACCTTATTC CTGAGCTTCG AGCTGTTTTG 
AGAAGGTTTT TCCTGCGGAT TGGTGTGGTT TTCCAAATAT CCCAACCACC AGAACAAGAG 
TGTGGAATAA GCAAGCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( XM_014873011.1 ) cDNA ORF clone, Sturnus vulgaris(Common starling) -> XP_014728497.1 Sturnus vulgaris ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.

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CloneID	OSt06345
Clone ID Related Accession (Same CDS sequence)	XM_014873011.1
Accession Version	XM_014873011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-01
Organism	Sturnus vulgaris(Common starling)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014650511.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sturnus vulgaris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAAG GGAAGAAGAC GAAAAACATG TTTCTGACCC GGGCCCTGGA GAAGATCCTG 
GCCGATAAGG AGGTGAAGAA GGCGCATCAC TCCCAACTGC GCAAAGCCTG CGAGGTGGCA 
CTAGAGGAAA TAAAGGCTGA AACAGAAAAA CAAAGTCCCC CTCATGGAGA AGCAAAGTCT 
GGTTCAAGCA CTCTTCCACC TGTGAAATCA AAGACCAGCT TCATTGAAGC AGACAAATAC 
TTCCTACCAT TTGAATTGGC ATGCCAGTCC AAGTGCCCCA GGATTGTCAG TACATCTCTA 
GATTGTTTAC AGAAACTTAT AGCATATGGG CACTTGACAG GGAATGCTCC AGACAGTACA 
GCTCCAGGAA AAAAACTAAT TGATAGAATT ATCGAGACAA TATGTGGCTG TTTTCAAGGT 
CCTCAAACAG ACGAAGGGGT TCAGCTACAA ATAATAAAGG CTTTGCTCAC TGCAGTAACA 
TCTCAACATA TAGAAATCCA CGAAGGAACA GTACTACAGG CTGTAAGAAC ATGTTACAAT 
ATCTACCTAG CCAGCAAAAA TCTTATCAAT CAAACTACAG CCAAGGCCAC TCTAACACAA 
ATGCTGAATG TCATTTTTGC ACGTATGGAA AATCAAGCAC TTCAGGAAGC CAAACAGATG 
GAAAAGGAAA GGCACAGACA GCAACACCAT CTCCAGCAGT CTCCAGTAAG CCATCATGAG 
CCTGAATCAC CTCAGTTGAG ACATATTCCA GCACAGGCTG ATCAGCTGTC CAAAGACCAT 
GAGAGAGAGC TTGACCACAG CACAAACGAT ATGGACAAGA ACCTTCAAGA AGATATTGAG 
GCAGAAAATG GATCTGACAT TTCTAGTGCT GAAAATGAAC AGACAGAAGC TGACCAAGCC 
ACAGCAGCAG AAAATCTTTC TAAAGCTGAT GGAGGAACAT ACGATGGTGA AAGCAATGAG 
TGTGAAGAGA AGGCACAAGA AATTGTAGAA AGTATTGTGC AGGAAATGGT GAACATAGTA 
GTTGGAGATG TGGAAGGGAC TGCAAGTGGT GATGGCACAA CTGTATCATT AGAGGATGGC 
AGTGACAGTG AAAACATTCA GGCAAATGGA ATTCCAGGGA CTCCTATTTC TGTTGCTTAT 
ACACCATCTT TACCCGATGA CAGATTATCT GTCTCTTCCA ACGATACTCA GGAATCTGGA 
AATTCTTCAG GACCTACCCC TGGTGCTAAG TTTTCCCACA TTTTACAAAA GGATGCCTTC 
CTTGTATTCA GGTCACTGTG TAAACTCTCT ATGAAGCCCC TGTCTGATGG ACCACCAGAT 
CCAAAATCTC ATGAACTGCG ATCAAAGATT CTTTCATTGC AGTTGCTTCT GTCCATTCTG 
CAAAATGCAG GACCAGTCTT CAGGACAAAT GAGATGTTCA TTAATGCTAT AAAGCAGTAC 
CTCTGTGTTG CACTGTCAAA AAATGGAGTC TCATCAGTTC CAGAAGTTTT TGAACTTTCA 
CTTTCCATAT TTCTTACCCT TCTGTCAAAC TTCAAGACTC ATCTAAAGAT GCAGATTGAG 
GTTTTCTTCA AAGAAATTTT CCTGTATATC TTGGAAACTT CTACTAGCTC ATTTGATCAT 
AAGTGGATGG TTATACAAAC GCTGACAAGG ATATGTGCAG ATGCTCAGAG CGTGGTGGAC 
ATCTACGTGA ACTATGATTG TGATTTAAAT GCAGCAAATA TATTTGAAAG GCTGGTTAAT 
GACTTGTCAA AGATTGCTCA AGGAAGGGGT AGCCAAGAAC TTGGCATGTC CAATGTTCAG 
GAATTAAGTT TGAGGAAAAA AGGATTAGAA TGCTTAGTAT CAATCTTGAA ATGCATGGTG 
GAGTGGAGTA AGGATCAATA TGTAAATCCC AATTCCCAGA CAACCCTTGG CCAAGAAAAA 
CCCACAGAAC AGGACAGCAG TGAAACCAAA CACCCTGAGA CAATTAACAG ATATGGAAGC 
TTAAATTCTT TGGATTCTAC TGCTTCATCA GGAATTGGCA GTTACAGCAC ACAGATGTCT 
GGCACTGACA ACCCAGAGCA GTTTGAAGTC CTAAAGCAGC AAAAGGAAAT AATAGAACAA 
GGAATTGACT TATTCAACAA GAAACCAAAG AGGGGGATTC AGTACCTACA AGAACAAGGA 
ATGCTTGGTA CTACCCCTGA AGATATTGCT CAATTCTTGC ATCAAGAGGA AAGATTAGAT 
TCAACTCAGG TTGGGGAGTT CCTGGGAGAC AACGATAAAT TTAACAAAGA AGTCATGTAT 
GCATATGTAG ACCAGCACGA CTTTTCGGGA AAGGATTTTG TTTCAGCTCT GCGCATGTTT 
CTAGAAGGAT TTCGTCTGCC AGGAGAAGCT CAAAAAATTG ATCGCTTAAT GGAGAAATTT 
GCTGCTAGAT ATTTAGAATG TAACCAAGGG CAAACTCTTT TTGCTAGTGC AGATACTGCA 
TATGTTTTAG CTTACTCAAT TATCATGTTG ACAACAGATC TTCACAGTCC ACAGGTTAAG 
AATAAAATGA CAAAAGAACA GTATATTAAA ATGAATAGAG GTATCAATGA CAGTAAAGAT 
CTCCCTGAAG AATATTTGTC TGCTATCTAT AATGAAATAG CTGGAAAGAA GATTTCAATG 
AAAGAAACAA AAGAATTAAC AATACCCACA AAGTCCAGTA AACAAAGTGT TGCTAGTGAG 
AAGCAGAGAA GACTTCTATA TAACTTGGAA ATGGAACAAA TGGCTAAAAC AGCTAAAGCT 
CTTATGGAGG CAGTGAGCCA CGTTCAGGCC CCTTTTACTA GTGCAACACA CCTGGAGCAT 
GTGAGACCCA TGTTTAAGTT GGCATGGACA CCTTTCCTGG CTGCATTCAG TGTGGGTCTG 
CAGGACTGTG ATGACACCGA AGTTGCCTCT CTTTGTTTGG AGGGTATACG ATGTGCAATC 
CGGATTGCTT GCATTTTCAA CATTCAGCTT GAAAGAGATG CCTATGTTCA AGCATTAGCA 
AGATTTACAT TGCTTACAGT GAGTTCTGGT ATTACTGAAA TGAAGCAGAA GAATATTGAC 
ACCATTAAAA CTCTCATCAC GGTGGCTCAT ACAGATGGAA ACTACTTAGG AAATTCCTGG 
CATGAGATTC TGAAATGCAT CAGTCAGCTT GAACTGGCAC AGTTAATAGG AACTGGAGTA 
AAACCTCGCT ACATCTCAGG GACAGTTCGT GGCAGGGAAG GTTCTTTTAC TGGAACAAAA 
GATCAGGCAC CTGATGAATT TGTGGGGCTG GGACTGGTTG GTGGAAACGT GGATTGGAAG 
CAGATAGCAA GTATTCAGGA ATCTATTGGT GAAACTAGCT CCCAAAGCGT TGTTGTTGCT 
GTAGACAGAA TATTTACGGG ATCTACAAGG TTGGATGGAA ATGCTATTGT GGATTTTGTT 
CGCTGGCTTT GTGCTGTGTC TATGGACGAG CTGCTGTCTG CTACCCACCC TCGAATGTTT 
AGTCTGCAGA AGATTGTAGA GATTTCTTAT TACAACATGG GCCGGATAAG GTTACAGTGG 
TCTAGGATCT GGGAAGTTAT TGGCGATCAT TTTAATAAGG TTGGCTGCAA TCCCAATGAA 
GATGTAGCTA TTTTTGCAGT GGACTCATTA AGGCAATTGT CAATGAAGTT CTTGGAAAAA 
GGAGAACTTG CTAATTTCCG ATTCCAGAAG GACTTCCTAA GACCATTTGA ACATATCATG 
AAGAGAAACA GGTCTCCTAC TATTCGAGAC ATGGTTGTGC GGTGTATTGC GCAGATGGTG 
AATTCCCAGG CTGCTAACAT TCGTTCTGGT TGGAAAAACA TTTTTTCAGT GTTTCATCTG 
GCAGCCTCAG ATCAGGATGA AAGTATAGTG GAACTGGCAT TCCAGACTAC AGGACACATT 
GTCACAATCG TGTTTGAAAA GCACTTCCCA GCGACCATAG ATTCTTTCCA GGATGCAGTG 
AAGTGCTTGT CTGAATTTGC CTGCAATGCA GCATTTCCAG ATACCAGTAT GGAAGCGATC 
CGCCTCATTC GCCACTGTGC AAAATATGTG TCTGACAGGC CTCAGGCATT CAAGGAATAC 
ACAAGTGATG ATATGAATGT AGCTCCTGAA GACAGAGTGT GGGTGCGAGG ATGGTTCCCA 
ATTCTGTTTG AGTTGTCCTG TATCATCAAT AGATGCAAAT TAGATGTAAG AACCAGAGGC 
TTAACAGTAA TGTTTGAAAT AATGAAGACA TATGGCCATA CTTATGAAAA ACACTGGTGG 
CAAGATTTGT TTCGGATTGT CTTCAGGATA TTTGACAACA TGAAACTGCC AGAACAGCAG 
ACTGAGAAAG CCGAATGGAT GACAACTACC TGTAACCATG CACTTTATGC AATATGTGAT 
GTATTTACAC AGTATTTAGA AGTACTTAGT GATGTTCTTT TGGATGATAT ATTTGCACAG 
CTGTACTGGT GTGTGCAGCA AGACAATGAA CAACTGGCAC GATCTGGTAC AAACTGTTTG 
GAGAATGTTG TCATCCTTAA TGGTGAAAAG TTCACCCTAG AAATCTGGGA TAAAACTTGC 
ACTTGCATGC TGGATATTTT CAAAACTACA ATCCCTCATG CGCTGCTGAC TTGGCGACCA 
GTCGGTGGGG AGTTCTGCTC TGGCTCTCCC TCTGATGCAA AAGAAAAACT GGATACAATC 
TCGCAGAAGT CAGTAGATAT CCATGATTCC ATCCAGCCTC GATCTTCAGA TGGGCGCCCG 
TATCAACCCA GCACAGGGCC CACGGTAGCT GAAGAAACGA GTAAAGCCAG GCCAGCAGCA 
AAATTCCCAG AACAGAAGTT ATTTGCTGCT CTTTTGATCA AGTGTGTTGT ACAGCTGGAA 
CTCATTCAGA CGATCGACAA CATTGTGTTC TTCCCAGCAA CTAGCAAAAA AGAGGATGCA 
GAGAATCTAG CAGCAGCACA AAGAGATGCA GTAGACTTCG ATGTCCATGT GGATACACAG 
GATCAAGGGA TGTATCGTTT CCTAACATCA CAGCAGCTTT TCAAACTTCT CGATTGTCTG 
CTGGAGTCTC ACAGATTTGC TAAAGCATTT AATTCAAACA ATGAACAGAG AACTGCTCTC 
TGGAAAGCAG GTTTCAAAGG CAAATCAAAG CCCAACCTGC TGAAGCAGGA GACGAGCAGC 
CTGGCCTGTG GGCTGCGCAT CCTGTTCCGC ATGTACATGG ACGAGAGCCG CACCAGCGCC 
TGGGAGGAAG TCCAGCAAAG GCTGCTGAAT GTCTGCAGCG AGGCTCTGAG CTATTTCCTC 
ACTCTTACAT CAGAAAGTCA CCGGGAAGCC TGGACTAACC TTTTACTCTT ATTTTTGACT 
AAAGTCCTGA AGATAAGTGA TGAAAGGTTC AAGGCTCATG CCTCATTCTA CTATCCTTTG 
TTGTGTGAAA TCATGCAGTT TGACCTTATT CCTGAGCTTC GAGCTGTTTT GAGAAGGTTT 
TTCCTGCGGA TTGGTGTGGT TTTCCAAATA TCCCAACCAC CAGAACAAGA GTGTGGAATA 
AGCAAGCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014728497.1
CDS	1..5532
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKSGSSTLPPVKSKTSFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTAPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQQHHLQQSPVSHHEPESPQLRHIPAQADQLSKDHERELDHSTNDMDKNLQEDIEAENGSDISSAENEQTEADQATAAENLSKADGGTYDGESNECEEKAQEIVESIVQEMVNIVVGDVEGTASGDGTTVSLEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPTPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPTEQDSSETKHPETINRYGSLNSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSSKQSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFNIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSFTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSATHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTIVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCTCMLDIFKTTIPHALLTWRPVGGEFCSGSPSDAKEKLDTISQKSVDIHDSIQPRSSDGRPYQPSTGPTVAEETSKARPAAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVHVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRTSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDERFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQECGISKH

Target ORF information:

RefSeq Version	XM_014873011.1
Organism	Sturnus vulgaris(Common starling)
Definition	Sturnus vulgaris ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014873011.1

ORF Insert Sequence:

ATGTATGAAG GGAAGAAGAC GAAAAACATG TTTCTGACCC GGGCCCTGGA GAAGATCCTG 
GCCGATAAGG AGGTGAAGAA GGCGCATCAC TCCCAACTGC GCAAAGCCTG CGAGGTGGCA 
CTAGAGGAAA TAAAGGCTGA AACAGAAAAA CAAAGTCCCC CTCATGGAGA AGCAAAGTCT 
GGTTCAAGCA CTCTTCCACC TGTGAAATCA AAGACCAGCT TCATTGAAGC AGACAAATAC 
TTCCTACCAT TTGAATTGGC ATGCCAGTCC AAGTGCCCCA GGATTGTCAG TACATCTCTA 
GATTGTTTAC AGAAACTTAT AGCATATGGG CACTTGACAG GGAATGCTCC AGACAGTACA 
GCTCCAGGAA AAAAACTAAT TGATAGAATT ATCGAGACAA TATGTGGCTG TTTTCAAGGT 
CCTCAAACAG ACGAAGGGGT TCAGCTACAA ATAATAAAGG CTTTGCTCAC TGCAGTAACA 
TCTCAACATA TAGAAATCCA CGAAGGAACA GTACTACAGG CTGTAAGAAC ATGTTACAAT 
ATCTACCTAG CCAGCAAAAA TCTTATCAAT CAAACTACAG CCAAGGCCAC TCTAACACAA 
ATGCTGAATG TCATTTTTGC ACGTATGGAA AATCAAGCAC TTCAGGAAGC CAAACAGATG 
GAAAAGGAAA GGCACAGACA GCAACACCAT CTCCAGCAGT CTCCAGTAAG CCATCATGAG 
CCTGAATCAC CTCAGTTGAG ACATATTCCA GCACAGGCTG ATCAGCTGTC CAAAGACCAT 
GAGAGAGAGC TTGACCACAG CACAAACGAT ATGGACAAGA ACCTTCAAGA AGATATTGAG 
GCAGAAAATG GATCTGACAT TTCTAGTGCT GAAAATGAAC AGACAGAAGC TGACCAAGCC 
ACAGCAGCAG AAAATCTTTC TAAAGCTGAT GGAGGAACAT ACGATGGTGA AAGCAATGAG 
TGTGAAGAGA AGGCACAAGA AATTGTAGAA AGTATTGTGC AGGAAATGGT GAACATAGTA 
GTTGGAGATG TGGAAGGGAC TGCAAGTGGT GATGGCACAA CTGTATCATT AGAGGATGGC 
AGTGACAGTG AAAACATTCA GGCAAATGGA ATTCCAGGGA CTCCTATTTC TGTTGCTTAT 
ACACCATCTT TACCCGATGA CAGATTATCT GTCTCTTCCA ACGATACTCA GGAATCTGGA 
AATTCTTCAG GACCTACCCC TGGTGCTAAG TTTTCCCACA TTTTACAAAA GGATGCCTTC 
CTTGTATTCA GGTCACTGTG TAAACTCTCT ATGAAGCCCC TGTCTGATGG ACCACCAGAT 
CCAAAATCTC ATGAACTGCG ATCAAAGATT CTTTCATTGC AGTTGCTTCT GTCCATTCTG 
CAAAATGCAG GACCAGTCTT CAGGACAAAT GAGATGTTCA TTAATGCTAT AAAGCAGTAC 
CTCTGTGTTG CACTGTCAAA AAATGGAGTC TCATCAGTTC CAGAAGTTTT TGAACTTTCA 
CTTTCCATAT TTCTTACCCT TCTGTCAAAC TTCAAGACTC ATCTAAAGAT GCAGATTGAG 
GTTTTCTTCA AAGAAATTTT CCTGTATATC TTGGAAACTT CTACTAGCTC ATTTGATCAT 
AAGTGGATGG TTATACAAAC GCTGACAAGG ATATGTGCAG ATGCTCAGAG CGTGGTGGAC 
ATCTACGTGA ACTATGATTG TGATTTAAAT GCAGCAAATA TATTTGAAAG GCTGGTTAAT 
GACTTGTCAA AGATTGCTCA AGGAAGGGGT AGCCAAGAAC TTGGCATGTC CAATGTTCAG 
GAATTAAGTT TGAGGAAAAA AGGATTAGAA TGCTTAGTAT CAATCTTGAA ATGCATGGTG 
GAGTGGAGTA AGGATCAATA TGTAAATCCC AATTCCCAGA CAACCCTTGG CCAAGAAAAA 
CCCACAGAAC AGGACAGCAG TGAAACCAAA CACCCTGAGA CAATTAACAG ATATGGAAGC 
TTAAATTCTT TGGATTCTAC TGCTTCATCA GGAATTGGCA GTTACAGCAC ACAGATGTCT 
GGCACTGACA ACCCAGAGCA GTTTGAAGTC CTAAAGCAGC AAAAGGAAAT AATAGAACAA 
GGAATTGACT TATTCAACAA GAAACCAAAG AGGGGGATTC AGTACCTACA AGAACAAGGA 
ATGCTTGGTA CTACCCCTGA AGATATTGCT CAATTCTTGC ATCAAGAGGA AAGATTAGAT 
TCAACTCAGG TTGGGGAGTT CCTGGGAGAC AACGATAAAT TTAACAAAGA AGTCATGTAT 
GCATATGTAG ACCAGCACGA CTTTTCGGGA AAGGATTTTG TTTCAGCTCT GCGCATGTTT 
CTAGAAGGAT TTCGTCTGCC AGGAGAAGCT CAAAAAATTG ATCGCTTAAT GGAGAAATTT 
GCTGCTAGAT ATTTAGAATG TAACCAAGGG CAAACTCTTT TTGCTAGTGC AGATACTGCA 
TATGTTTTAG CTTACTCAAT TATCATGTTG ACAACAGATC TTCACAGTCC ACAGGTTAAG 
AATAAAATGA CAAAAGAACA GTATATTAAA ATGAATAGAG GTATCAATGA CAGTAAAGAT 
CTCCCTGAAG AATATTTGTC TGCTATCTAT AATGAAATAG CTGGAAAGAA GATTTCAATG 
AAAGAAACAA AAGAATTAAC AATACCCACA AAGTCCAGTA AACAAAGTGT TGCTAGTGAG 
AAGCAGAGAA GACTTCTATA TAACTTGGAA ATGGAACAAA TGGCTAAAAC AGCTAAAGCT 
CTTATGGAGG CAGTGAGCCA CGTTCAGGCC CCTTTTACTA GTGCAACACA CCTGGAGCAT 
GTGAGACCCA TGTTTAAGTT GGCATGGACA CCTTTCCTGG CTGCATTCAG TGTGGGTCTG 
CAGGACTGTG ATGACACCGA AGTTGCCTCT CTTTGTTTGG AGGGTATACG ATGTGCAATC 
CGGATTGCTT GCATTTTCAA CATTCAGCTT GAAAGAGATG CCTATGTTCA AGCATTAGCA 
AGATTTACAT TGCTTACAGT GAGTTCTGGT ATTACTGAAA TGAAGCAGAA GAATATTGAC 
ACCATTAAAA CTCTCATCAC GGTGGCTCAT ACAGATGGAA ACTACTTAGG AAATTCCTGG 
CATGAGATTC TGAAATGCAT CAGTCAGCTT GAACTGGCAC AGTTAATAGG AACTGGAGTA 
AAACCTCGCT ACATCTCAGG GACAGTTCGT GGCAGGGAAG GTTCTTTTAC TGGAACAAAA 
GATCAGGCAC CTGATGAATT TGTGGGGCTG GGACTGGTTG GTGGAAACGT GGATTGGAAG 
CAGATAGCAA GTATTCAGGA ATCTATTGGT GAAACTAGCT CCCAAAGCGT TGTTGTTGCT 
GTAGACAGAA TATTTACGGG ATCTACAAGG TTGGATGGAA ATGCTATTGT GGATTTTGTT 
CGCTGGCTTT GTGCTGTGTC TATGGACGAG CTGCTGTCTG CTACCCACCC TCGAATGTTT 
AGTCTGCAGA AGATTGTAGA GATTTCTTAT TACAACATGG GCCGGATAAG GTTACAGTGG 
TCTAGGATCT GGGAAGTTAT TGGCGATCAT TTTAATAAGG TTGGCTGCAA TCCCAATGAA 
GATGTAGCTA TTTTTGCAGT GGACTCATTA AGGCAATTGT CAATGAAGTT CTTGGAAAAA 
GGAGAACTTG CTAATTTCCG ATTCCAGAAG GACTTCCTAA GACCATTTGA ACATATCATG 
AAGAGAAACA GGTCTCCTAC TATTCGAGAC ATGGTTGTGC GGTGTATTGC GCAGATGGTG 
AATTCCCAGG CTGCTAACAT TCGTTCTGGT TGGAAAAACA TTTTTTCAGT GTTTCATCTG 
GCAGCCTCAG ATCAGGATGA AAGTATAGTG GAACTGGCAT TCCAGACTAC AGGACACATT 
GTCACAATCG TGTTTGAAAA GCACTTCCCA GCGACCATAG ATTCTTTCCA GGATGCAGTG 
AAGTGCTTGT CTGAATTTGC CTGCAATGCA GCATTTCCAG ATACCAGTAT GGAAGCGATC 
CGCCTCATTC GCCACTGTGC AAAATATGTG TCTGACAGGC CTCAGGCATT CAAGGAATAC 
ACAAGTGATG ATATGAATGT AGCTCCTGAA GACAGAGTGT GGGTGCGAGG ATGGTTCCCA 
ATTCTGTTTG AGTTGTCCTG TATCATCAAT AGATGCAAAT TAGATGTAAG AACCAGAGGC 
TTAACAGTAA TGTTTGAAAT AATGAAGACA TATGGCCATA CTTATGAAAA ACACTGGTGG 
CAAGATTTGT TTCGGATTGT CTTCAGGATA TTTGACAACA TGAAACTGCC AGAACAGCAG 
ACTGAGAAAG CCGAATGGAT GACAACTACC TGTAACCATG CACTTTATGC AATATGTGAT 
GTATTTACAC AGTATTTAGA AGTACTTAGT GATGTTCTTT TGGATGATAT ATTTGCACAG 
CTGTACTGGT GTGTGCAGCA AGACAATGAA CAACTGGCAC GATCTGGTAC AAACTGTTTG 
GAGAATGTTG TCATCCTTAA TGGTGAAAAG TTCACCCTAG AAATCTGGGA TAAAACTTGC 
ACTTGCATGC TGGATATTTT CAAAACTACA ATCCCTCATG CGCTGCTGAC TTGGCGACCA 
GTCGGTGGGG AGTTCTGCTC TGGCTCTCCC TCTGATGCAA AAGAAAAACT GGATACAATC 
TCGCAGAAGT CAGTAGATAT CCATGATTCC ATCCAGCCTC GATCTTCAGA TGGGCGCCCG 
TATCAACCCA GCACAGGGCC CACGGTAGCT GAAGAAACGA GTAAAGCCAG GCCAGCAGCA 
AAATTCCCAG AACAGAAGTT ATTTGCTGCT CTTTTGATCA AGTGTGTTGT ACAGCTGGAA 
CTCATTCAGA CGATCGACAA CATTGTGTTC TTCCCAGCAA CTAGCAAAAA AGAGGATGCA 
GAGAATCTAG CAGCAGCACA AAGAGATGCA GTAGACTTCG ATGTCCATGT GGATACACAG 
GATCAAGGGA TGTATCGTTT CCTAACATCA CAGCAGCTTT TCAAACTTCT CGATTGTCTG 
CTGGAGTCTC ACAGATTTGC TAAAGCATTT AATTCAAACA ATGAACAGAG AACTGCTCTC 
TGGAAAGCAG GTTTCAAAGG CAAATCAAAG CCCAACCTGC TGAAGCAGGA GACGAGCAGC 
CTGGCCTGTG GGCTGCGCAT CCTGTTCCGC ATGTACATGG ACGAGAGCCG CACCAGCGCC 
TGGGAGGAAG TCCAGCAAAG GCTGCTGAAT GTCTGCAGCG AGGCTCTGAG CTATTTCCTC 
ACTCTTACAT CAGAAAGTCA CCGGGAAGCC TGGACTAACC TTTTACTCTT ATTTTTGACT 
AAAGTCCTGA AGATAAGTGA TGAAAGGTTC AAGGCTCATG CCTCATTCTA CTATCCTTTG 
TTGTGTGAAA TCATGCAGTT TGACCTTATT CCTGAGCTTC GAGCTGTTTT GAGAAGGTTT 
TTCCTGCGGA TTGGTGTGGT TTTCCAAATA TCCCAACCAC CAGAACAAGA GTGTGGAATA 
AGCAAGCATT AG