grem1 cDNA ORF clone,

Gene
Clones
gRNAs

The following grem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGi29669	XM_030405179.1 Latest version!	Sparus aurata gremlin 1, DAN family BMP antagonist (grem1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grem1 ( XM_030405179.1 ) cDNA ORF clone, -> XP_030261039.1 Sparus aurata gremlin 1, DAN family BMP antagonist (grem1), mRNA.

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CloneID	OGi29669
Clone ID Related Accession (Same CDS sequence)	XM_030405179.1
Accession Version	XM_030405179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-17
Organism	Sparus aurata(gilthead seabream)
Product	gremlin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044208.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sparus aurata Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAC CGTCGGTGCT GATCTCCACA GCAGTTCTGG CGCTGCTGCT CAGCTCGCCG 
AGGAGTACGG ACGCTGACAC CTTTCAAGGC GCTATCCCTC ACCCGAACAA GTACGACCCA 
CAGGAACCGG ATAGATGCCA ACAACCAGCC ATCGGTGGAC TAATTTCCCG CGGAGCAGGG 
CCCGTGACCT CCACCGACGA GGTGCTGGAG TCCAGCCAGG AGGCGCTGCA CGTCACGGAG 
CGTCGCTACC TGCGGCTGGA CTGGTGCAAG ACGCAGCCGC TGAAGCAGAC CATCCGCGAG 
GAGGGCTGCC TGAGCCGCAC CATCATCAAC CGCTTCTGCT ACGGACAGTG CAACTCCTTC 
TACATCCCGA GGCACAACTA CCAGGACGGG GACGTCTTTC AGTCCTGCTC TGCGTGCAAA 
CCCAAAACCT TCAGCACCGT CACGTACACC CTCTTCTGCC CGGGTCAGAC GCCCAGCACC 
AGGAAGAAAC GGATCCAGCG CGTAAAGCTG TGCCGCTGCA CGACCATAGA TACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030261039.1
CDS	92..631
Translation	MKTPSVLISTAVLALLLSSPRSTDADTFQGAIPHPNKYDPQEPDRCQQPAIGGLISRGAGPVTSTDEVLESSQEALHVTERRYLRLDWCKTQPLKQTIREEGCLSRTIINRFCYGQCNSFYIPRHNYQDGDVFQSCSACKPKTFSTVTYTLFCPGQTPSTRKKRIQRVKLCRCTTIDTD

Target ORF information:

RefSeq Version	XM_030405179.1
Organism	Sparus aurata(gilthead seabream)
Definition	Sparus aurata gremlin 1, DAN family BMP antagonist (grem1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030405179.1

ORF Insert Sequence:

ATGAAGACAC CGTCGGTGCT GATCTCCACA GCAGTTCTGG CGCTGCTGCT CAGCTCGCCG 
AGGAGTACGG ACGCTGACAC CTTTCAAGGC GCTATCCCTC ACCCGAACAA GTACGACCCA 
CAGGAACCGG ATAGATGCCA ACAACCAGCC ATCGGTGGAC TAATTTCCCG CGGAGCAGGG 
CCCGTGACCT CCACCGACGA GGTGCTGGAG TCCAGCCAGG AGGCGCTGCA CGTCACGGAG 
CGTCGCTACC TGCGGCTGGA CTGGTGCAAG ACGCAGCCGC TGAAGCAGAC CATCCGCGAG 
GAGGGCTGCC TGAGCCGCAC CATCATCAAC CGCTTCTGCT ACGGACAGTG CAACTCCTTC 
TACATCCCGA GGCACAACTA CCAGGACGGG GACGTCTTTC AGTCCTGCTC TGCGTGCAAA 
CCCAAAACCT TCAGCACCGT CACGTACACC CTCTTCTGCC CGGGTCAGAC GCCCAGCACC 
AGGAAGAAAC GGATCCAGCG CGTAAAGCTG TGCCGCTGCA CGACCATAGA TACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.