gba2 cDNA ORF clone, Seriola lalandi dorsalis

Gene
Clones
gRNAs

Summary

Gene Symbol	gba2
Entrez Gene ID	111647183
Full Name	glucosylceramidase beta 2
Gene Type	protein-coding
Organism	Seriola lalandi dorsalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_023396795.1	XP_023252563.1	non-lysosomal glucosylceramidase
XM_023396796.1	XP_023252564.1	non-lysosomal glucosylceramidase

Pathways from BioSystems
Gene Ontology

The following gba2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gba2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe177061	XM_023396796.1 Latest version!	Seriola lalandi dorsalis glucosylceramidase beta 2 (gba2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OSe177061	XM_023396795.1 Latest version!	Seriola lalandi dorsalis glucosylceramidase beta 2 (gba2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gba2 ( XM_023396795.1 ) cDNA ORF clone, Seriola lalandi dorsalis -> XP_023252563.1 Seriola lalandi dorsalis glucosylceramidase beta 2 (gba2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe177061
Clone ID Related Accession (Same CDS sequence)	XM_023396795.1 , XM_023396796.1
Accession Version	XM_023396795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-25
Organism	Seriola lalandi dorsalis
Product	non-lysosomal glucosylceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019525532.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Seriola lalandi dorsalis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCGG AGTGGGGTGA GAAATCCACA GCGGACCTCA TGAGTAGGTA TGTCTCCAAG 
GAAATGGGAT ACGGAGTGCC CAAGGAAGGT TGGCGCATCT GTCTGGCACA TGAGTTCAAG 
GAGAAGAGAA AGCCCTTTCA AGCAAAAGAT GTCTCGCTGT CCAATGTCTG GGAGCACGTT 
GGCCTTGGAA TCAGGTATCT AAAATGGTGG TACAAGAAGA CCCAGGTGGA AAAGAAGGCT 
CCGTTCATCG ATATGTTTGG TGCCCAACCG TTGCGTCAAA TATATGGTGC TCCACTTGGC 
GGTATTGGAG GAGGTACCAT CACAAGAGGT TGGAGGGGAG AGTTCTGTCG ATGGCAACTT 
AACCCTGGAA TGTACCACTA CAAAACTGTC ACAGCCAACC AGTTCACAGT TTGTTTGCGT 
CGTGGAGGAC AAACAGTTTA CCAGCAGGTG CTATCTGTAG AGCGTCCTCC CACGTTACAA 
GGCTGGAACT GGGGCTACTG TGGGGAGTAT GCCTTCTACC ACGCCCTGTA CCCCCGCGCC 
TGGACTGTGT ACCACCTGCC GGGACAGAAC GTCACCCTGA CCTGCAGACA GATTTCCCCT 
GTCATCCCCC ATGATTACCA GGACTCAAGT CTCCCGGTGG CTGTATTTGT GTGGGACATA 
GAGAACAAGA ATGACTACGA CTTAGATGTC TCCATAATGT TTACTATGGT CAATGGGTCG 
GGACACAAGG ACGACAAGAG CGGGGGACAC TGGAATGAAC CATTCCACCT GGAGAAGGAG 
GGGGAGGCAG TGTCTGGGGT TTTGCTACAT CACTGCACTA CAGTAAACCC ATACACTCTG 
TGCATTGCAG CCCGAGAACA GCCTGACAGG GAGGTCAGTC ACCAGACAGC GTTCAGCCCA 
AAGGGAACTT GCAGCAGTCT GTGGAGTGAC CTCATCACTG ATGGACGACT GGACTCTCCT 
ACAGGTTCCT GCCCGCCGAC GCCTAGGGGA GAGAAGGTGG CAGCAGCGTT GGCAGTGGGC 
TGCTCGGTGT CGGCTCAGAG CTGTAACACC CTGGAGTTCT GTCTGACTTG GGACATGCCC 
AAGATCACCT TCGGTTCCAG GGAGAGGGAA CACATTAGGA GATATACTCG TTACTTTGGG 
ACCAAAGGGG ATGCGTCCCC CTCTCTCAGT CACTACGCCC TAACACACTA CAAACAGTGG 
GAGAAGAGCA TTGAGGAGTG GCAGAGACCG ATTCTGCGGG ACAGTTCTCT CCCTTCCTGG 
TATAAATCGG CTCTGTTTAA TGAGTTGTAC TTTGTGGTGG ATGGAGGGTC GGTGTGGACT 
GAGCTACCAG AGGATGCTGA TGTCAGTGGC GGCGTGCGCA GTGAAGAGGG GGGGCTCCCA 
GCTCAGCCTG CTGTCATCAA GGAGTACGGC CGTTTTGCCT ACCTAGAAGG TCAGGAGTAC 
AGAATGTACA ACACATACGA TGTGCACTTC TACGCTTCTT TTGCACTTAT CATGCTGTGG 
CCAAAACTTG CCTTGAGTCT GCAATATGAT ATTGCTGGCA GTGTGGTTCA GCAGGACCCA 
ACAGAAAGGC TCCATCTGAT GAGTGGGCGG TATTCTCCAG TCAAGGCCAA AAATGTGGTG 
CCTCATGACA TAGGAGACCC AGATGATGAG CCATGGCAAA GGGTGAATGC CTACCTCATT 
CATGACACTG CAGACTGGAA GGACTTGAAC CTGAAGTTTG TCCTGCAGGT CTACAGGGAC 
TTTCATCTTA CCCAGGACAG TCAGTACCTG CGGGACATGT GGCCCATCTG CCAGGCGGTC 
ATGGAGTCGG AGATAAAGTT TGACCTAGAT GGCGATGGAC TAATAGAAAA CTCTGGATAT 
GCTGACCAGA CCTATGATGG CTGGACAGTG ACTGGACCAA GTGCGTACTG TGGTGGGCTG 
TGGTTGGCGT CCCTGTGTGT GATGTGTAAG ATGGCCAGAT TGGTAGACAA CGAGGAGAAA 
TACCAATATT ACAGAGACCT CTTGGACAGA GGCAGTGCTG CTTTTGACCA ACTGCTGTGG 
AACGGCAAGT ACTACAACTA TGATAGCAGT GGGAGAGACC TTTCCAACAG TGTCATGTCT 
GACCAGTGTG CTGGCCACTG GTTCCTGAGA GCGTCTGGGC TGGGAGAGGG AGACTACCAG 
GCTTTTCCAA AAGAAAAAAT CCAGACCGCA CTAAAATCTG TCTTTGACTT GAATGTAATG 
AGCTTTGCTG GAGGCCAGAT GGGGGCAGTT AACGGCATGC GTCCTGAAGG AGTGCCTGAC 
CGCTCCAGCG TCCAATCAGA TGAGGTCTGG ATTGGAGTAG TGTATGGACT GGCAGCCACT 
ATGATCCATG AGGGTATGCG GGATGAGGGT TTGCGCACGG CGGAGGGTTG CTACCGGACT 
GTGTGGGAAA GACTAGGCAT GGCGTTCCAG ACTCCTGAAG CCTACTGTGA GAAGGGCATC 
TATCGCTCTC TGGCCTACAT GAGGCCTCTG AGCGTATGGG CCATGCAGCT AGCCCTAAAC 
ACTTCACAGA AGGATCAGAC CACATCAGCT CAAACTGGAG ACATTGGCAG CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023252563.1
CDS	959..3535
Translation	MSSEWGEKSTADLMSRYVSKEMGYGVPKEGWRICLAHEFKEKRKPFQAKDVSLSNVWEHVGLGIRYLKWWYKKTQVEKKAPFIDMFGAQPLRQIYGAPLGGIGGGTITRGWRGEFCRWQLNPGMYHYKTVTANQFTVCLRRGGQTVYQQVLSVERPPTLQGWNWGYCGEYAFYHALYPRAWTVYHLPGQNVTLTCRQISPVIPHDYQDSSLPVAVFVWDIENKNDYDLDVSIMFTMVNGSGHKDDKSGGHWNEPFHLEKEGEAVSGVLLHHCTTVNPYTLCIAAREQPDREVSHQTAFSPKGTCSSLWSDLITDGRLDSPTGSCPPTPRGEKVAAALAVGCSVSAQSCNTLEFCLTWDMPKITFGSREREHIRRYTRYFGTKGDASPSLSHYALTHYKQWEKSIEEWQRPILRDSSLPSWYKSALFNELYFVVDGGSVWTELPEDADVSGGVRSEEGGLPAQPAVIKEYGRFAYLEGQEYRMYNTYDVHFYASFALIMLWPKLALSLQYDIAGSVVQQDPTERLHLMSGRYSPVKAKNVVPHDIGDPDDEPWQRVNAYLIHDTADWKDLNLKFVLQVYRDFHLTQDSQYLRDMWPICQAVMESEIKFDLDGDGLIENSGYADQTYDGWTVTGPSAYCGGLWLASLCVMCKMARLVDNEEKYQYYRDLLDRGSAAFDQLLWNGKYYNYDSSGRDLSNSVMSDQCAGHWFLRASGLGEGDYQAFPKEKIQTALKSVFDLNVMSFAGGQMGAVNGMRPEGVPDRSSVQSDEVWIGVVYGLAATMIHEGMRDEGLRTAEGCYRTVWERLGMAFQTPEAYCEKGIYRSLAYMRPLSVWAMQLALNTSQKDQTTSAQTGDIGSI

Target ORF information:

RefSeq Version	XM_023396795.1
Organism	Seriola lalandi dorsalis
Definition	Seriola lalandi dorsalis glucosylceramidase beta 2 (gba2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023396795.1

ORF Insert Sequence:

ATGTCTTCGG AGTGGGGTGA GAAATCCACA GCGGACCTCA TGAGTAGGTA TGTCTCCAAG 
GAAATGGGAT ACGGAGTGCC CAAGGAAGGT TGGCGCATCT GTCTGGCACA TGAGTTCAAG 
GAGAAGAGAA AGCCCTTTCA AGCAAAAGAT GTCTCGCTGT CCAATGTCTG GGAGCACGTT 
GGCCTTGGAA TCAGGTATCT AAAATGGTGG TACAAGAAGA CCCAGGTGGA AAAGAAGGCT 
CCGTTCATCG ATATGTTTGG TGCCCAACCG TTGCGTCAAA TATATGGTGC TCCACTTGGC 
GGTATTGGAG GAGGTACCAT CACAAGAGGT TGGAGGGGAG AGTTCTGTCG ATGGCAACTT 
AACCCTGGAA TGTACCACTA CAAAACTGTC ACAGCCAACC AGTTCACAGT TTGTTTGCGT 
CGTGGAGGAC AAACAGTTTA CCAGCAGGTG CTATCTGTAG AGCGTCCTCC CACGTTACAA 
GGCTGGAACT GGGGCTACTG TGGGGAGTAT GCCTTCTACC ACGCCCTGTA CCCCCGCGCC 
TGGACTGTGT ACCACCTGCC GGGACAGAAC GTCACCCTGA CCTGCAGACA GATTTCCCCT 
GTCATCCCCC ATGATTACCA GGACTCAAGT CTCCCGGTGG CTGTATTTGT GTGGGACATA 
GAGAACAAGA ATGACTACGA CTTAGATGTC TCCATAATGT TTACTATGGT CAATGGGTCG 
GGACACAAGG ACGACAAGAG CGGGGGACAC TGGAATGAAC CATTCCACCT GGAGAAGGAG 
GGGGAGGCAG TGTCTGGGGT TTTGCTACAT CACTGCACTA CAGTAAACCC ATACACTCTG 
TGCATTGCAG CCCGAGAACA GCCTGACAGG GAGGTCAGTC ACCAGACAGC GTTCAGCCCA 
AAGGGAACTT GCAGCAGTCT GTGGAGTGAC CTCATCACTG ATGGACGACT GGACTCTCCT 
ACAGGTTCCT GCCCGCCGAC GCCTAGGGGA GAGAAGGTGG CAGCAGCGTT GGCAGTGGGC 
TGCTCGGTGT CGGCTCAGAG CTGTAACACC CTGGAGTTCT GTCTGACTTG GGACATGCCC 
AAGATCACCT TCGGTTCCAG GGAGAGGGAA CACATTAGGA GATATACTCG TTACTTTGGG 
ACCAAAGGGG ATGCGTCCCC CTCTCTCAGT CACTACGCCC TAACACACTA CAAACAGTGG 
GAGAAGAGCA TTGAGGAGTG GCAGAGACCG ATTCTGCGGG ACAGTTCTCT CCCTTCCTGG 
TATAAATCGG CTCTGTTTAA TGAGTTGTAC TTTGTGGTGG ATGGAGGGTC GGTGTGGACT 
GAGCTACCAG AGGATGCTGA TGTCAGTGGC GGCGTGCGCA GTGAAGAGGG GGGGCTCCCA 
GCTCAGCCTG CTGTCATCAA GGAGTACGGC CGTTTTGCCT ACCTAGAAGG TCAGGAGTAC 
AGAATGTACA ACACATACGA TGTGCACTTC TACGCTTCTT TTGCACTTAT CATGCTGTGG 
CCAAAACTTG CCTTGAGTCT GCAATATGAT ATTGCTGGCA GTGTGGTTCA GCAGGACCCA 
ACAGAAAGGC TCCATCTGAT GAGTGGGCGG TATTCTCCAG TCAAGGCCAA AAATGTGGTG 
CCTCATGACA TAGGAGACCC AGATGATGAG CCATGGCAAA GGGTGAATGC CTACCTCATT 
CATGACACTG CAGACTGGAA GGACTTGAAC CTGAAGTTTG TCCTGCAGGT CTACAGGGAC 
TTTCATCTTA CCCAGGACAG TCAGTACCTG CGGGACATGT GGCCCATCTG CCAGGCGGTC 
ATGGAGTCGG AGATAAAGTT TGACCTAGAT GGCGATGGAC TAATAGAAAA CTCTGGATAT 
GCTGACCAGA CCTATGATGG CTGGACAGTG ACTGGACCAA GTGCGTACTG TGGTGGGCTG 
TGGTTGGCGT CCCTGTGTGT GATGTGTAAG ATGGCCAGAT TGGTAGACAA CGAGGAGAAA 
TACCAATATT ACAGAGACCT CTTGGACAGA GGCAGTGCTG CTTTTGACCA ACTGCTGTGG 
AACGGCAAGT ACTACAACTA TGATAGCAGT GGGAGAGACC TTTCCAACAG TGTCATGTCT 
GACCAGTGTG CTGGCCACTG GTTCCTGAGA GCGTCTGGGC TGGGAGAGGG AGACTACCAG 
GCTTTTCCAA AAGAAAAAAT CCAGACCGCA CTAAAATCTG TCTTTGACTT GAATGTAATG 
AGCTTTGCTG GAGGCCAGAT GGGGGCAGTT AACGGCATGC GTCCTGAAGG AGTGCCTGAC 
CGCTCCAGCG TCCAATCAGA TGAGGTCTGG ATTGGAGTAG TGTATGGACT GGCAGCCACT 
ATGATCCATG AGGGTATGCG GGATGAGGGT TTGCGCACGG CGGAGGGTTG CTACCGGACT 
GTGTGGGAAA GACTAGGCAT GGCGTTCCAG ACTCCTGAAG CCTACTGTGA GAAGGGCATC 
TATCGCTCTC TGGCCTACAT GAGGCCTCTG AGCGTATGGG CCATGCAGCT AGCCCTAAAC 
ACTTCACAGA AGGATCAGAC CACATCAGCT CAAACTGGAG ACATTGGCAG CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gba2 ( XM_023396796.1 ) cDNA ORF clone, Seriola lalandi dorsalis -> XP_023252564.1 Seriola lalandi dorsalis glucosylceramidase beta 2 (gba2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe177061
Clone ID Related Accession (Same CDS sequence)	XM_023396795.1 , XM_023396796.1
Accession Version	XM_023396796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-25
Organism	Seriola lalandi dorsalis
Product	non-lysosomal glucosylceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019525532.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Seriola lalandi dorsalis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCGG AGTGGGGTGA GAAATCCACA GCGGACCTCA TGAGTAGGTA TGTCTCCAAG 
GAAATGGGAT ACGGAGTGCC CAAGGAAGGT TGGCGCATCT GTCTGGCACA TGAGTTCAAG 
GAGAAGAGAA AGCCCTTTCA AGCAAAAGAT GTCTCGCTGT CCAATGTCTG GGAGCACGTT 
GGCCTTGGAA TCAGGTATCT AAAATGGTGG TACAAGAAGA CCCAGGTGGA AAAGAAGGCT 
CCGTTCATCG ATATGTTTGG TGCCCAACCG TTGCGTCAAA TATATGGTGC TCCACTTGGC 
GGTATTGGAG GAGGTACCAT CACAAGAGGT TGGAGGGGAG AGTTCTGTCG ATGGCAACTT 
AACCCTGGAA TGTACCACTA CAAAACTGTC ACAGCCAACC AGTTCACAGT TTGTTTGCGT 
CGTGGAGGAC AAACAGTTTA CCAGCAGGTG CTATCTGTAG AGCGTCCTCC CACGTTACAA 
GGCTGGAACT GGGGCTACTG TGGGGAGTAT GCCTTCTACC ACGCCCTGTA CCCCCGCGCC 
TGGACTGTGT ACCACCTGCC GGGACAGAAC GTCACCCTGA CCTGCAGACA GATTTCCCCT 
GTCATCCCCC ATGATTACCA GGACTCAAGT CTCCCGGTGG CTGTATTTGT GTGGGACATA 
GAGAACAAGA ATGACTACGA CTTAGATGTC TCCATAATGT TTACTATGGT CAATGGGTCG 
GGACACAAGG ACGACAAGAG CGGGGGACAC TGGAATGAAC CATTCCACCT GGAGAAGGAG 
GGGGAGGCAG TGTCTGGGGT TTTGCTACAT CACTGCACTA CAGTAAACCC ATACACTCTG 
TGCATTGCAG CCCGAGAACA GCCTGACAGG GAGGTCAGTC ACCAGACAGC GTTCAGCCCA 
AAGGGAACTT GCAGCAGTCT GTGGAGTGAC CTCATCACTG ATGGACGACT GGACTCTCCT 
ACAGGTTCCT GCCCGCCGAC GCCTAGGGGA GAGAAGGTGG CAGCAGCGTT GGCAGTGGGC 
TGCTCGGTGT CGGCTCAGAG CTGTAACACC CTGGAGTTCT GTCTGACTTG GGACATGCCC 
AAGATCACCT TCGGTTCCAG GGAGAGGGAA CACATTAGGA GATATACTCG TTACTTTGGG 
ACCAAAGGGG ATGCGTCCCC CTCTCTCAGT CACTACGCCC TAACACACTA CAAACAGTGG 
GAGAAGAGCA TTGAGGAGTG GCAGAGACCG ATTCTGCGGG ACAGTTCTCT CCCTTCCTGG 
TATAAATCGG CTCTGTTTAA TGAGTTGTAC TTTGTGGTGG ATGGAGGGTC GGTGTGGACT 
GAGCTACCAG AGGATGCTGA TGTCAGTGGC GGCGTGCGCA GTGAAGAGGG GGGGCTCCCA 
GCTCAGCCTG CTGTCATCAA GGAGTACGGC CGTTTTGCCT ACCTAGAAGG TCAGGAGTAC 
AGAATGTACA ACACATACGA TGTGCACTTC TACGCTTCTT TTGCACTTAT CATGCTGTGG 
CCAAAACTTG CCTTGAGTCT GCAATATGAT ATTGCTGGCA GTGTGGTTCA GCAGGACCCA 
ACAGAAAGGC TCCATCTGAT GAGTGGGCGG TATTCTCCAG TCAAGGCCAA AAATGTGGTG 
CCTCATGACA TAGGAGACCC AGATGATGAG CCATGGCAAA GGGTGAATGC CTACCTCATT 
CATGACACTG CAGACTGGAA GGACTTGAAC CTGAAGTTTG TCCTGCAGGT CTACAGGGAC 
TTTCATCTTA CCCAGGACAG TCAGTACCTG CGGGACATGT GGCCCATCTG CCAGGCGGTC 
ATGGAGTCGG AGATAAAGTT TGACCTAGAT GGCGATGGAC TAATAGAAAA CTCTGGATAT 
GCTGACCAGA CCTATGATGG CTGGACAGTG ACTGGACCAA GTGCGTACTG TGGTGGGCTG 
TGGTTGGCGT CCCTGTGTGT GATGTGTAAG ATGGCCAGAT TGGTAGACAA CGAGGAGAAA 
TACCAATATT ACAGAGACCT CTTGGACAGA GGCAGTGCTG CTTTTGACCA ACTGCTGTGG 
AACGGCAAGT ACTACAACTA TGATAGCAGT GGGAGAGACC TTTCCAACAG TGTCATGTCT 
GACCAGTGTG CTGGCCACTG GTTCCTGAGA GCGTCTGGGC TGGGAGAGGG AGACTACCAG 
GCTTTTCCAA AAGAAAAAAT CCAGACCGCA CTAAAATCTG TCTTTGACTT GAATGTAATG 
AGCTTTGCTG GAGGCCAGAT GGGGGCAGTT AACGGCATGC GTCCTGAAGG AGTGCCTGAC 
CGCTCCAGCG TCCAATCAGA TGAGGTCTGG ATTGGAGTAG TGTATGGACT GGCAGCCACT 
ATGATCCATG AGGGTATGCG GGATGAGGGT TTGCGCACGG CGGAGGGTTG CTACCGGACT 
GTGTGGGAAA GACTAGGCAT GGCGTTCCAG ACTCCTGAAG CCTACTGTGA GAAGGGCATC 
TATCGCTCTC TGGCCTACAT GAGGCCTCTG AGCGTATGGG CCATGCAGCT AGCCCTAAAC 
ACTTCACAGA AGGATCAGAC CACATCAGCT CAAACTGGAG ACATTGGCAG CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023252564.1
CDS	673..3249
Translation	MSSEWGEKSTADLMSRYVSKEMGYGVPKEGWRICLAHEFKEKRKPFQAKDVSLSNVWEHVGLGIRYLKWWYKKTQVEKKAPFIDMFGAQPLRQIYGAPLGGIGGGTITRGWRGEFCRWQLNPGMYHYKTVTANQFTVCLRRGGQTVYQQVLSVERPPTLQGWNWGYCGEYAFYHALYPRAWTVYHLPGQNVTLTCRQISPVIPHDYQDSSLPVAVFVWDIENKNDYDLDVSIMFTMVNGSGHKDDKSGGHWNEPFHLEKEGEAVSGVLLHHCTTVNPYTLCIAAREQPDREVSHQTAFSPKGTCSSLWSDLITDGRLDSPTGSCPPTPRGEKVAAALAVGCSVSAQSCNTLEFCLTWDMPKITFGSREREHIRRYTRYFGTKGDASPSLSHYALTHYKQWEKSIEEWQRPILRDSSLPSWYKSALFNELYFVVDGGSVWTELPEDADVSGGVRSEEGGLPAQPAVIKEYGRFAYLEGQEYRMYNTYDVHFYASFALIMLWPKLALSLQYDIAGSVVQQDPTERLHLMSGRYSPVKAKNVVPHDIGDPDDEPWQRVNAYLIHDTADWKDLNLKFVLQVYRDFHLTQDSQYLRDMWPICQAVMESEIKFDLDGDGLIENSGYADQTYDGWTVTGPSAYCGGLWLASLCVMCKMARLVDNEEKYQYYRDLLDRGSAAFDQLLWNGKYYNYDSSGRDLSNSVMSDQCAGHWFLRASGLGEGDYQAFPKEKIQTALKSVFDLNVMSFAGGQMGAVNGMRPEGVPDRSSVQSDEVWIGVVYGLAATMIHEGMRDEGLRTAEGCYRTVWERLGMAFQTPEAYCEKGIYRSLAYMRPLSVWAMQLALNTSQKDQTTSAQTGDIGSI

Target ORF information:

RefSeq Version	XM_023396796.1
Organism	Seriola lalandi dorsalis
Definition	Seriola lalandi dorsalis glucosylceramidase beta 2 (gba2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023396796.1

ORF Insert Sequence:

ATGTCTTCGG AGTGGGGTGA GAAATCCACA GCGGACCTCA TGAGTAGGTA TGTCTCCAAG 
GAAATGGGAT ACGGAGTGCC CAAGGAAGGT TGGCGCATCT GTCTGGCACA TGAGTTCAAG 
GAGAAGAGAA AGCCCTTTCA AGCAAAAGAT GTCTCGCTGT CCAATGTCTG GGAGCACGTT 
GGCCTTGGAA TCAGGTATCT AAAATGGTGG TACAAGAAGA CCCAGGTGGA AAAGAAGGCT 
CCGTTCATCG ATATGTTTGG TGCCCAACCG TTGCGTCAAA TATATGGTGC TCCACTTGGC 
GGTATTGGAG GAGGTACCAT CACAAGAGGT TGGAGGGGAG AGTTCTGTCG ATGGCAACTT 
AACCCTGGAA TGTACCACTA CAAAACTGTC ACAGCCAACC AGTTCACAGT TTGTTTGCGT 
CGTGGAGGAC AAACAGTTTA CCAGCAGGTG CTATCTGTAG AGCGTCCTCC CACGTTACAA 
GGCTGGAACT GGGGCTACTG TGGGGAGTAT GCCTTCTACC ACGCCCTGTA CCCCCGCGCC 
TGGACTGTGT ACCACCTGCC GGGACAGAAC GTCACCCTGA CCTGCAGACA GATTTCCCCT 
GTCATCCCCC ATGATTACCA GGACTCAAGT CTCCCGGTGG CTGTATTTGT GTGGGACATA 
GAGAACAAGA ATGACTACGA CTTAGATGTC TCCATAATGT TTACTATGGT CAATGGGTCG 
GGACACAAGG ACGACAAGAG CGGGGGACAC TGGAATGAAC CATTCCACCT GGAGAAGGAG 
GGGGAGGCAG TGTCTGGGGT TTTGCTACAT CACTGCACTA CAGTAAACCC ATACACTCTG 
TGCATTGCAG CCCGAGAACA GCCTGACAGG GAGGTCAGTC ACCAGACAGC GTTCAGCCCA 
AAGGGAACTT GCAGCAGTCT GTGGAGTGAC CTCATCACTG ATGGACGACT GGACTCTCCT 
ACAGGTTCCT GCCCGCCGAC GCCTAGGGGA GAGAAGGTGG CAGCAGCGTT GGCAGTGGGC 
TGCTCGGTGT CGGCTCAGAG CTGTAACACC CTGGAGTTCT GTCTGACTTG GGACATGCCC 
AAGATCACCT TCGGTTCCAG GGAGAGGGAA CACATTAGGA GATATACTCG TTACTTTGGG 
ACCAAAGGGG ATGCGTCCCC CTCTCTCAGT CACTACGCCC TAACACACTA CAAACAGTGG 
GAGAAGAGCA TTGAGGAGTG GCAGAGACCG ATTCTGCGGG ACAGTTCTCT CCCTTCCTGG 
TATAAATCGG CTCTGTTTAA TGAGTTGTAC TTTGTGGTGG ATGGAGGGTC GGTGTGGACT 
GAGCTACCAG AGGATGCTGA TGTCAGTGGC GGCGTGCGCA GTGAAGAGGG GGGGCTCCCA 
GCTCAGCCTG CTGTCATCAA GGAGTACGGC CGTTTTGCCT ACCTAGAAGG TCAGGAGTAC 
AGAATGTACA ACACATACGA TGTGCACTTC TACGCTTCTT TTGCACTTAT CATGCTGTGG 
CCAAAACTTG CCTTGAGTCT GCAATATGAT ATTGCTGGCA GTGTGGTTCA GCAGGACCCA 
ACAGAAAGGC TCCATCTGAT GAGTGGGCGG TATTCTCCAG TCAAGGCCAA AAATGTGGTG 
CCTCATGACA TAGGAGACCC AGATGATGAG CCATGGCAAA GGGTGAATGC CTACCTCATT 
CATGACACTG CAGACTGGAA GGACTTGAAC CTGAAGTTTG TCCTGCAGGT CTACAGGGAC 
TTTCATCTTA CCCAGGACAG TCAGTACCTG CGGGACATGT GGCCCATCTG CCAGGCGGTC 
ATGGAGTCGG AGATAAAGTT TGACCTAGAT GGCGATGGAC TAATAGAAAA CTCTGGATAT 
GCTGACCAGA CCTATGATGG CTGGACAGTG ACTGGACCAA GTGCGTACTG TGGTGGGCTG 
TGGTTGGCGT CCCTGTGTGT GATGTGTAAG ATGGCCAGAT TGGTAGACAA CGAGGAGAAA 
TACCAATATT ACAGAGACCT CTTGGACAGA GGCAGTGCTG CTTTTGACCA ACTGCTGTGG 
AACGGCAAGT ACTACAACTA TGATAGCAGT GGGAGAGACC TTTCCAACAG TGTCATGTCT 
GACCAGTGTG CTGGCCACTG GTTCCTGAGA GCGTCTGGGC TGGGAGAGGG AGACTACCAG 
GCTTTTCCAA AAGAAAAAAT CCAGACCGCA CTAAAATCTG TCTTTGACTT GAATGTAATG 
AGCTTTGCTG GAGGCCAGAT GGGGGCAGTT AACGGCATGC GTCCTGAAGG AGTGCCTGAC 
CGCTCCAGCG TCCAATCAGA TGAGGTCTGG ATTGGAGTAG TGTATGGACT GGCAGCCACT 
ATGATCCATG AGGGTATGCG GGATGAGGGT TTGCGCACGG CGGAGGGTTG CTACCGGACT 
GTGTGGGAAA GACTAGGCAT GGCGTTCCAG ACTCCTGAAG CCTACTGTGA GAAGGGCATC 
TATCGCTCTC TGGCCTACAT GAGGCCTCTG AGCGTATGGG CCATGCAGCT AGCCCTAAAC 
ACTTCACAGA AGGATCAGAC CACATCAGCT CAAACTGGAG ACATTGGCAG CATTTGA