rnf31 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	rnf31
Entrez Gene ID	111238327
Full Name	ring finger protein 31
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022767766.1	XP_022623487.1	E3 ubiquitin-protein ligase RNF31

Pathways from BioSystems
Gene Ontology

The following rnf31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rnf31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe196950	XM_022767766.1 Latest version!	Seriola dumerili ring finger protein 31 (rnf31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rnf31 ( XM_022767766.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022623487.1 Seriola dumerili ring finger protein 31 (rnf31), mRNA.

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CloneID	OSe196950
Clone ID Related Accession (Same CDS sequence)	XM_022767766.1
Accession Version	XM_022767766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	E3 ubiquitin-protein ligase RNF31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019175553.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA GCTCTCCCAC TGTAGAGATG GACGAGGTCA GGAGCAGAGC CCAGTCCCTC 
CTCTCCTCCT CGGGCTCAGC CCAGAATGTG AAGACTGAGG TCCAGACCAT GGCCGGCATC 
GCTCTGCCTC TGTCAGAGAA GTACCATCAC CTCACAGCGG AGGCCATGCT GACGGAGAAC 
ACAGCCGACC GCAACAGACT GGAGGCGCTG GAGTCTTTGA GTAGACTGGT CAAGGCCTTG 
AGTATCCTGG AGAAGTACGG CTGTAACCTG ACCTGCCCTG CCAGGCCCAG ATACTGGAGG 
AGCGTCAAGC ACAACAACCC CGTCTTCAGG ACCACGGTGG ACGCCATCAA GGGAGGGCGG 
CGAGTCCTTT TTCTCTACGG TTACACCAAC CAGCAGATAG ATGGCCTCAG TTTCCCAGAT 
GAAGTCAGCG AACCAGATGC TGAGAAAGTG GCTGCAGTGA CGCTGGAGGT CATGACCTTA 
CGCACGGAGG TGGACATGAT CATTAAGGGC ACCCATCCTC ACCTAGAAAC CTTCAAAGAC 
ATCCTCCCAT TTCTCATGCA GCAGGACACG GTGGTGATTG ACTCTGTGGC GGTCCCGCCG 
GGAGAGGAGC AGCAGCTTGG AGACAAACCC CAGGTGCTCT CTCCTTCCCC TAAACCCGCC 
CCGCACCTCG TAGCACAGCC CCTGAAATCA TCAACAGGCC CTCTTGCAGC CAGCGAGTGT 
AATTTGTGTG GCAGCGCCAC GTCTCTGGTT TGCCCGTCTT GCGACAATCA AGCCTTTTGC 
GACGCGTGTG ATATCCTCTT CCACCGCCAT CCCTCCAGAG CCAATCACAA GAGGGACAAA 
TTACAGAAAA CCAACCAGGA GAGCTGCAGT ATCTGTGGCA TCTCTGCTGT CTACGCTCAC 
TGCTCCACCT GTGTCCAGAG ATTGTGTGTG AACTGTGACA AGCTCTTCCA CTCTCACCCC 
GACCGCAAAG GACACAACAG AACGATCGTC TCACCTGCCA AAACATCCAG TCCCTCTTTG 
TCGCCCTGGG AATGCGCTCA TTGCACCACA GTGAACGAGA TGCGAGCTGT TCTCTGCTCC 
ACCTGTGAAC GGCCTCGACT CGCCACGGCT GCTTCCACCG TTCAGGAGAG TCCCATGTCA 
GTTCCTACGT CACCAAACAC AGAGTGGCAG TGTAAAAGCT GCACTGTCGT AAATCAAGGC 
AGCAGCATCC TGTGTGAGGT GTGTGAGCGC CCCCGTCTGG CCACTCGGCC GCCCATCGCC 
CCAGTGTCGT CCAACCCAGG GTCCCTGACT GATACTGGAG ACAAGTGGAC TTGTCAGTTC 
TGCACCTACG TGAACACCAA GCCCACGTTG GTGTGTGAGA TGTGCAACCT GCCCTGTAAA 
GACTCTCCTG GCGATTCTCT GCCCGAGCCT CTTCAGCAGA CTCCGTCCAT CACTAAGGAT 
CAACCTCAGC CCGGCGGGAA GCCGCTGCCG AAGCCCAGAA TGAACCTGGA GCTGAAGAGA 
CAGAAGACGA TGAGGGACGA CGGACTCACC CTCATCCACC AGATCAGAGA AGCAGAAAAG 
CGAGGCGTCA GCCCAGAGGA GGTGTACGCA GCCCTCTGCA TGTGCGGCGG CAGCAACATC 
AACCCCTGTG ATTGGCTGAC GTCCGAGCTG CCTCACCTGC TGGATGAAAT CTGCGCCATG 
GCAGCGTCTG TCCAGCTAAA CTACAGAGCA GGGGATTGTG GGATACTACC CGTGGTGGAG 
AAAGACGGAG AGGAGTCGGA GGAGAGCGGC CCTAACGCCA CAGGTGAAGA TGTGAAGCTG 
TCCAGAGCTG AAGCCAAGCT GGCATGGTTA GCAGCGGGAG GAGACACCGA CAGGGCCGTG 
ACACAGCTGC TGAGAGACCG ACAGGTCAAG ATGAGGGAGC TCCACTCTCT GGGTTTCAGG 
GATGTGGTCA TGTGTGAGGA GGCCCTGCGG CAGAGCGGAG GTGAAGTCAA AGGGGCTTTG 
TCTTTGCTTC AGCGCCCCCT CCTGGAGCCT TTCCACCAGC GTATCTGGAC CGACCAGCCC 
GAGCCGCCGA TTGATCCAAA GCACCCGGAC AAACAGAGGA TGTGCAGACG TCTGCTGGCG 
TTGTACGACC TGCCCAGCTG GGGGCGCTGT GAGCTCGTGC TGTCGCTGCT CCAGGAGCCA 
GGCGTCACTT ACTCCCTGGA GGATGTGGTG CAAGCTGTGA AGGAGTCACA TGACAAAGAC 
TTCATCAGGC GTCTCCTCAA CAATGAGTGC CCCTGCTGTC TGTCCATCTT TCCCCGCATC 
AAGATGCAAT CTCTGACTTC CTGTCAGTGC TCAGTGTGCC ACGAGTGTTT CGGGCAACAC 
TTCACCATCG CTGTGAGAGA CAAACACATC CGAGACATGG TGTGTCCCGT CTGCGGAGAG 
CCCGACATCA ACGACCCAGA ACAACTGGAC AGTTACTTCT CCACGCTGGA CATACAGCTG 
CGGGACTGCC TGGAGCCAGA GGTGTACGAT CTGTTTCATA AAAAACTGAC GGAGCACGCT 
CTAATGAAAG ACCCAAAGTT CCTCTGGTGC TGTCATTGCA CCTCTGGGTT TATCAATGAT 
GGAGACCAGC TGAAGGTCAC CTGCCTGTCA TGCCGGAAAA GTTTTTGCGC TCAGTGCAAG 
AAACCTTGGG AGCCTCAGCA CCAGGATCTG TCCTGTGAAC AGTTCCAGCA GTGGAAGAGG 
GAGAACGATC CCGAGTACCA GAGGCAGGGC CTGGCCGGCT ACCTGAGAGA CAACGGCATC 
ACCTGTCCTC ACTGCAGGTT CCAATACGCT CTGACGAAAG GCGGCTGCAT GCACTTCAGC 
TGCTCCCAGT GCAGGTACCA GTTCTGCAGC GGCTGCAACA ACCCCTACCA CAAGACGGGA 
TGTAAAACGC CCCAGTGCAA AATGACTGGT CTACACGCCC ATCATCCTCG TGACTGTCTC 
TTCTATCTAA GAGACTGGGA GCCGCCCAGA CTACAGGCCC TGCTGCAGAG GAGCGGTGTG 
GAGTTCAACA CAGACCCTTC TAATGGAACT CAAAGTGACT CTTGTGGTGT AATGGAGCAG 
AAAGATGAAG GAGGTCAGCA GACTGATTCA CCGTGTGGCA TTCAAACACA GCCGGGACAA 
GCTGGGCTCT GCGAGAAGCA CTACAGAGAA TACCTGGTCA GTCTGATAAA CGATCACTCT 
CTGGACCCGG CTCTGCTCTA CGACGCCCAC GAGCTGACTC GGGCCTGTGA GAGATACGAG 
GTGGACGCAC AGAGTAGAGA GAACGAGGAT GACAACGCTT ACCATGCTCG ACTGCTCAAG 
AAACTGATGG AGGTTCCTCT TGGAGAGAAG GTTCCCCGCA ACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022623487.1
CDS	179..3526
Translation	MSSSSPTVEMDEVRSRAQSLLSSSGSAQNVKTEVQTMAGIALPLSEKYHHLTAEAMLTENTADRNRLEALESLSRLVKALSILEKYGCNLTCPARPRYWRSVKHNNPVFRTTVDAIKGGRRVLFLYGYTNQQIDGLSFPDEVSEPDAEKVAAVTLEVMTLRTEVDMIIKGTHPHLETFKDILPFLMQQDTVVIDSVAVPPGEEQQLGDKPQVLSPSPKPAPHLVAQPLKSSTGPLAASECNLCGSATSLVCPSCDNQAFCDACDILFHRHPSRANHKRDKLQKTNQESCSICGISAVYAHCSTCVQRLCVNCDKLFHSHPDRKGHNRTIVSPAKTSSPSLSPWECAHCTTVNEMRAVLCSTCERPRLATAASTVQESPMSVPTSPNTEWQCKSCTVVNQGSSILCEVCERPRLATRPPIAPVSSNPGSLTDTGDKWTCQFCTYVNTKPTLVCEMCNLPCKDSPGDSLPEPLQQTPSITKDQPQPGGKPLPKPRMNLELKRQKTMRDDGLTLIHQIREAEKRGVSPEEVYAALCMCGGSNINPCDWLTSELPHLLDEICAMAASVQLNYRAGDCGILPVVEKDGEESEESGPNATGEDVKLSRAEAKLAWLAAGGDTDRAVTQLLRDRQVKMRELHSLGFRDVVMCEEALRQSGGEVKGALSLLQRPLLEPFHQRIWTDQPEPPIDPKHPDKQRMCRRLLALYDLPSWGRCELVLSLLQEPGVTYSLEDVVQAVKESHDKDFIRRLLNNECPCCLSIFPRIKMQSLTSCQCSVCHECFGQHFTIAVRDKHIRDMVCPVCGEPDINDPEQLDSYFSTLDIQLRDCLEPEVYDLFHKKLTEHALMKDPKFLWCCHCTSGFINDGDQLKVTCLSCRKSFCAQCKKPWEPQHQDLSCEQFQQWKRENDPEYQRQGLAGYLRDNGITCPHCRFQYALTKGGCMHFSCSQCRYQFCSGCNNPYHKTGCKTPQCKMTGLHAHHPRDCLFYLRDWEPPRLQALLQRSGVEFNTDPSNGTQSDSCGVMEQKDEGGQQTDSPCGIQTQPGQAGLCEKHYREYLVSLINDHSLDPALLYDAHELTRACERYEVDAQSRENEDDNAYHARLLKKLMEVPLGEKVPRNK

Target ORF information:

RefSeq Version	XM_022767766.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili ring finger protein 31 (rnf31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022767766.1

ORF Insert Sequence:

ATGAGCAGCA GCTCTCCCAC TGTAGAGATG GACGAGGTCA GGAGCAGAGC CCAGTCCCTC 
CTCTCCTCCT CGGGCTCAGC CCAGAATGTG AAGACTGAGG TCCAGACCAT GGCCGGCATC 
GCTCTGCCTC TGTCAGAGAA GTACCATCAC CTCACAGCGG AGGCCATGCT GACGGAGAAC 
ACAGCCGACC GCAACAGACT GGAGGCGCTG GAGTCTTTGA GTAGACTGGT CAAGGCCTTG 
AGTATCCTGG AGAAGTACGG CTGTAACCTG ACCTGCCCTG CCAGGCCCAG ATACTGGAGG 
AGCGTCAAGC ACAACAACCC CGTCTTCAGG ACCACGGTGG ACGCCATCAA GGGAGGGCGG 
CGAGTCCTTT TTCTCTACGG TTACACCAAC CAGCAGATAG ATGGCCTCAG TTTCCCAGAT 
GAAGTCAGCG AACCAGATGC TGAGAAAGTG GCTGCAGTGA CGCTGGAGGT CATGACCTTA 
CGCACGGAGG TGGACATGAT CATTAAGGGC ACCCATCCTC ACCTAGAAAC CTTCAAAGAC 
ATCCTCCCAT TTCTCATGCA GCAGGACACG GTGGTGATTG ACTCTGTGGC GGTCCCGCCG 
GGAGAGGAGC AGCAGCTTGG AGACAAACCC CAGGTGCTCT CTCCTTCCCC TAAACCCGCC 
CCGCACCTCG TAGCACAGCC CCTGAAATCA TCAACAGGCC CTCTTGCAGC CAGCGAGTGT 
AATTTGTGTG GCAGCGCCAC GTCTCTGGTT TGCCCGTCTT GCGACAATCA AGCCTTTTGC 
GACGCGTGTG ATATCCTCTT CCACCGCCAT CCCTCCAGAG CCAATCACAA GAGGGACAAA 
TTACAGAAAA CCAACCAGGA GAGCTGCAGT ATCTGTGGCA TCTCTGCTGT CTACGCTCAC 
TGCTCCACCT GTGTCCAGAG ATTGTGTGTG AACTGTGACA AGCTCTTCCA CTCTCACCCC 
GACCGCAAAG GACACAACAG AACGATCGTC TCACCTGCCA AAACATCCAG TCCCTCTTTG 
TCGCCCTGGG AATGCGCTCA TTGCACCACA GTGAACGAGA TGCGAGCTGT TCTCTGCTCC 
ACCTGTGAAC GGCCTCGACT CGCCACGGCT GCTTCCACCG TTCAGGAGAG TCCCATGTCA 
GTTCCTACGT CACCAAACAC AGAGTGGCAG TGTAAAAGCT GCACTGTCGT AAATCAAGGC 
AGCAGCATCC TGTGTGAGGT GTGTGAGCGC CCCCGTCTGG CCACTCGGCC GCCCATCGCC 
CCAGTGTCGT CCAACCCAGG GTCCCTGACT GATACTGGAG ACAAGTGGAC TTGTCAGTTC 
TGCACCTACG TGAACACCAA GCCCACGTTG GTGTGTGAGA TGTGCAACCT GCCCTGTAAA 
GACTCTCCTG GCGATTCTCT GCCCGAGCCT CTTCAGCAGA CTCCGTCCAT CACTAAGGAT 
CAACCTCAGC CCGGCGGGAA GCCGCTGCCG AAGCCCAGAA TGAACCTGGA GCTGAAGAGA 
CAGAAGACGA TGAGGGACGA CGGACTCACC CTCATCCACC AGATCAGAGA AGCAGAAAAG 
CGAGGCGTCA GCCCAGAGGA GGTGTACGCA GCCCTCTGCA TGTGCGGCGG CAGCAACATC 
AACCCCTGTG ATTGGCTGAC GTCCGAGCTG CCTCACCTGC TGGATGAAAT CTGCGCCATG 
GCAGCGTCTG TCCAGCTAAA CTACAGAGCA GGGGATTGTG GGATACTACC CGTGGTGGAG 
AAAGACGGAG AGGAGTCGGA GGAGAGCGGC CCTAACGCCA CAGGTGAAGA TGTGAAGCTG 
TCCAGAGCTG AAGCCAAGCT GGCATGGTTA GCAGCGGGAG GAGACACCGA CAGGGCCGTG 
ACACAGCTGC TGAGAGACCG ACAGGTCAAG ATGAGGGAGC TCCACTCTCT GGGTTTCAGG 
GATGTGGTCA TGTGTGAGGA GGCCCTGCGG CAGAGCGGAG GTGAAGTCAA AGGGGCTTTG 
TCTTTGCTTC AGCGCCCCCT CCTGGAGCCT TTCCACCAGC GTATCTGGAC CGACCAGCCC 
GAGCCGCCGA TTGATCCAAA GCACCCGGAC AAACAGAGGA TGTGCAGACG TCTGCTGGCG 
TTGTACGACC TGCCCAGCTG GGGGCGCTGT GAGCTCGTGC TGTCGCTGCT CCAGGAGCCA 
GGCGTCACTT ACTCCCTGGA GGATGTGGTG CAAGCTGTGA AGGAGTCACA TGACAAAGAC 
TTCATCAGGC GTCTCCTCAA CAATGAGTGC CCCTGCTGTC TGTCCATCTT TCCCCGCATC 
AAGATGCAAT CTCTGACTTC CTGTCAGTGC TCAGTGTGCC ACGAGTGTTT CGGGCAACAC 
TTCACCATCG CTGTGAGAGA CAAACACATC CGAGACATGG TGTGTCCCGT CTGCGGAGAG 
CCCGACATCA ACGACCCAGA ACAACTGGAC AGTTACTTCT CCACGCTGGA CATACAGCTG 
CGGGACTGCC TGGAGCCAGA GGTGTACGAT CTGTTTCATA AAAAACTGAC GGAGCACGCT 
CTAATGAAAG ACCCAAAGTT CCTCTGGTGC TGTCATTGCA CCTCTGGGTT TATCAATGAT 
GGAGACCAGC TGAAGGTCAC CTGCCTGTCA TGCCGGAAAA GTTTTTGCGC TCAGTGCAAG 
AAACCTTGGG AGCCTCAGCA CCAGGATCTG TCCTGTGAAC AGTTCCAGCA GTGGAAGAGG 
GAGAACGATC CCGAGTACCA GAGGCAGGGC CTGGCCGGCT ACCTGAGAGA CAACGGCATC 
ACCTGTCCTC ACTGCAGGTT CCAATACGCT CTGACGAAAG GCGGCTGCAT GCACTTCAGC 
TGCTCCCAGT GCAGGTACCA GTTCTGCAGC GGCTGCAACA ACCCCTACCA CAAGACGGGA 
TGTAAAACGC CCCAGTGCAA AATGACTGGT CTACACGCCC ATCATCCTCG TGACTGTCTC 
TTCTATCTAA GAGACTGGGA GCCGCCCAGA CTACAGGCCC TGCTGCAGAG GAGCGGTGTG 
GAGTTCAACA CAGACCCTTC TAATGGAACT CAAAGTGACT CTTGTGGTGT AATGGAGCAG 
AAAGATGAAG GAGGTCAGCA GACTGATTCA CCGTGTGGCA TTCAAACACA GCCGGGACAA 
GCTGGGCTCT GCGAGAAGCA CTACAGAGAA TACCTGGTCA GTCTGATAAA CGATCACTCT 
CTGGACCCGG CTCTGCTCTA CGACGCCCAC GAGCTGACTC GGGCCTGTGA GAGATACGAG 
GTGGACGCAC AGAGTAGAGA GAACGAGGAT GACAACGCTT ACCATGCTCG ACTGCTCAAG 
AAACTGATGG AGGTTCCTCT TGGAGAGAAG GTTCCCCGCA ACAAATAA