ulk1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	ulk1
Entrez Gene ID	111233068
Full Name	unc-51 like autophagy activating kinase 1
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022760812.1	XP_022616533.1	serine/threonine-protein kinase ULK1 isoform X2
XM_022760811.1	XP_022616532.1	serine/threonine-protein kinase ULK1 isoform X1

Pathways from BioSystems
Gene Ontology

The following ulk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ulk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe191271	XM_022760811.1 Latest version!	Seriola dumerili unc-51 like autophagy activating kinase 1 (ulk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSe191272	XM_022760812.1 Latest version!	Seriola dumerili unc-51 like autophagy activating kinase 1 (ulk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ulk1 ( XM_022760811.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022616532.1 Seriola dumerili unc-51 like autophagy activating kinase 1 (ulk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe191271
Clone ID Related Accession (Same CDS sequence)	XM_022760811.1
Accession Version	XM_022760811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	serine/threonine-protein kinase ULK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174620.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGG TGGGGAAATT TGAGTTCAGT CGGAAGGACT TGATAGGACA TGGCGCATTT 
GCTGTCGTCT TCAAAGGCAG GCATCGAGAG AAACATGACT GGGAGGTGGC AGTAAAATGC 
ATAAACAAGA AGAACCTGGC CAAATCCCAG ACACTGTTGG GGAAGGAGAT CAAAATACTC 
AAGGAACTAA AGCATGAAAA CATTGTTGCA TTACTGGACT TTCAGGAAAC TGCCAGCTCA 
GTGTACCTGG TAATGGAGTA CTGCAATGGG GGTGACCTTG CTGACTACTT ACACTCCAAA 
GGCACACTGA GTGAGGACAC TATCCGGGTT TTCCTGCAGC AGATTGCAGG GGCCATGAGG 
GTCCTACAGG CCAAAGGCAT AATCCACAGG GACCTCAAAC CCCAGAATAT TCTGCTCTCC 
TACCCACCAG GACGCAAGTC GCACTCCAAC AACACCTGCA TCAAGATTGC GGACTTTGGC 
TTTGCCAGGT ACCTTCAGAA CAACATGATG GCGGCGACAC TCTGTGGGTC CCCAATGTAC 
ATGGCTCCTG AGGTCATTAT GTCCCAAAAC TATGATGCCA AGGCTGACTT GTGGAGTATA 
GGAACCATAG TGTTTCAGTG CCTGACTGGA AAGGCTCCTT TTCAGGCTAG CAGTCCCCAG 
GACCTCCGGC TGTTCTATGA AAAAAACAAG AATCTCAGCC CCAACATCCC CAGGGAGACT 
TCAAGCCATT TGAGGCACCT GCTGCTGGGA CTGCTGCAGC GCAACCACAA GGACCGCATG 
GACTTTGATG AGTTTTTTGG TCATTCTTTC CTGGAGGCTA GCTCATCAAT GAAAAAGATA 
ACTCCAACTG TGACCATGAC CTGCTTCCCA AGCTCTGCAT CAGCCAGCTC TTGTAGCAGC 
TCTTCCACAT CTCATCTCGC CTCACCACCG CAGTCTCTTG CTGAGGTTCA GCATTTGCGT 
GCCAAAGCTC TGGCCTCTCC TACCCAGGAG GCCACTGGTT TTCTCCTCAA GGACTCGTCT 
GGAGGGGGCG GCAGCAGCAA GAACTCCTCC TCCTGTGACA CAGATGACTT TGTCATGGTG 
CCTGCTCACT TCACGACAGG TGAGCTGACA TGTGAGAGTA AAGTGCTGCA GGACAGCCTG 
ATGAACAGTG GCTCTCTTCT GGCATCTGCT GGTCTGTGTA GCCAAGCCAA AACACCCCCA 
CACTCACCTT CCTACAGTGG CTCTCCAAGT CCTGTCAGGC CCAGTGAGTT CTCGGGAAGT 
AACTACGGTG GTAACTATGG AAACCATGGT CAGTCATTGC CTATTCCAGT CCCCACTCAG 
GTCCAGAACT ACCAGCGCAT GGAGCAGAAT CTCCACTCTA CCAAACAGGA TGGCTCACCA 
CGGCTGTCGA CACCGGTGCG TCGTTGCAGC AGTGGGAGCT CATTGGGCTT TGCTCGGACC 
GGACCTTCAC CATCTTACGG GCAGGGAGCA GTCACCGCCA CCCGCAGGCT CTCCCTGGGA 
GGTGGCAGAA CTTTCCAGCT CTCGCCTCTA GCTCCTCAGC TCACTGAACC CCGGCCGACT 
TCTCAGCAAC ACCCACAGAC GACAGGACTA GGCACACGGC TCCACAGTGC CCCCTGTCTG 
TTGGAGTGTG TCAGTGGTGG CGGGAGGCAG AAGATCAGGA AGCAGTACTC GGACCCTGTG 
GTTGCCCCCT CATCAGGCCT GATGACTGTC CGCCCCCTAC ACTCTTCCCC CAGATTGAGT 
GAGCTGATGC AGCGCAGCCC CCTCCCCACC ATCCTGGGCT CCCCTTCCAG GACCATCCCT 
CCATTTGAAT TCCCTAAGCC TCCCAGCTCT CCAAACCTTG TGACCTTCCT TACACAGCAG 
GGTTTGGTCA TCGGCTCTCC TGGCAGCAGG ACTGCCCTGA CAGAGCCCAG AGAGCCAGGA 
CAACAAGCAC CTACGCAGGT CGCCCAGCCT GCACACTGCA TCCACAGACT GAATGATGAT 
ACCAGGAGCT TCGGAAGGTC TCAGAGTGCT GGTCGTCTGT CTGACATGCT GCTGATGGCT 
GCGTTTGGAC CAGGTGGACC TGTGGGTGAA CGAGGCAGCA CAGAGAACCT GACCTCTGAA 
AAAGCCATAG AAATAACAGC GCCCCCAGGT GCTGGGGTAG GTCTTGCACC TGGATCCAGC 
AGCCCAGCAC AAGTGGTTTT CACTGTGGGC TCTCCTCCCA GTGGCAGCAC CCCACCTCAA 
ACTTCCAGAC AGAGGAAATA CTCAGGCTCG TTCACTTCAG TCAGCCCTGC AGGCTCCTTC 
ACCAGCCGGT ACCCCCAGAC AGGCACCTAT CTGGATGGTT TCGAGGCTCC TTCCAGTCCT 
CGTTACAGTT TCACTGATCC TATCACAGCC AACATGGGAG GCCCCGTAAC CTTTGAGGCT 
CCTGAGCTGC CTGAGGAGAC ACTGATGGAG CAGGAGCATA CAGACATGGT GCAAAACCTG 
CGTTTCACGT TGGATTTTGC ACGTTGTCTA ATGGAAGTGG CCGGGGCCCG TGGTGCTGCG 
GTGTTGGGGG AGCAGGTGGA CATTTCTCAT CCCTCCCTCC TTCAGCAGCA TAGCCTGGTA 
GCAGATCAGA TAAGCTCACT GAGCCGAGAG TGGAGTCATG CAGAACAGCT GGTTCTTTAC 
CTTAAGACTG CAGAACTCTT GTCGACTGCC TTACACACAG CCATGGAGCG AGTTAAACAG 
GGCAAACTCT ACCCATCTGC TACAGTCAAA CAAGTGGTGA GGAGGCTGAA TGAGCTGTAC 
AAATCCAGCG TGGCATCCTG TCGCTCACTC AGCACTCGTC TGGAGCGCTT TTTTTCCAGG 
AAGCACCGTC TTATGGACCA AATCACCTCC ATCACAGCAG AGCGGCTGCT GTTCAGCCAC 
ACTGTGCAGA TGGTTCAGGC TGCTGCGCTG GACGAGATGT TCCACCAGGG GGAAGCATCA 
GTCCTGCGCT ACCATAAAGC TCTGCTGCTG ATGGAGGGCT TGTCTCTGCT GCTCACTGAA 
CAGGATGACA TCCTCAGTGT TAGCAAATGC AAAGAATGCA TTGAACGCCG CCTCACAGCT 
TTGCAGTCAG GACTCTGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022616532.1
CDS	548..3631
Translation	METVGKFEFSRKDLIGHGAFAVVFKGRHREKHDWEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALLDFQETASSVYLVMEYCNGGDLADYLHSKGTLSEDTIRVFLQQIAGAMRVLQAKGIIHRDLKPQNILLSYPPGRKSHSNNTCIKIADFGFARYLQNNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVFQCLTGKAPFQASSPQDLRLFYEKNKNLSPNIPRETSSHLRHLLLGLLQRNHKDRMDFDEFFGHSFLEASSSMKKITPTVTMTCFPSSASASSCSSSSTSHLASPPQSLAEVQHLRAKALASPTQEATGFLLKDSSGGGGSSKNSSSCDTDDFVMVPAHFTTGELTCESKVLQDSLMNSGSLLASAGLCSQAKTPPHSPSYSGSPSPVRPSEFSGSNYGGNYGNHGQSLPIPVPTQVQNYQRMEQNLHSTKQDGSPRLSTPVRRCSSGSSLGFARTGPSPSYGQGAVTATRRLSLGGGRTFQLSPLAPQLTEPRPTSQQHPQTTGLGTRLHSAPCLLECVSGGGRQKIRKQYSDPVVAPSSGLMTVRPLHSSPRLSELMQRSPLPTILGSPSRTIPPFEFPKPPSSPNLVTFLTQQGLVIGSPGSRTALTEPREPGQQAPTQVAQPAHCIHRLNDDTRSFGRSQSAGRLSDMLLMAAFGPGGPVGERGSTENLTSEKAIEITAPPGAGVGLAPGSSSPAQVVFTVGSPPSGSTPPQTSRQRKYSGSFTSVSPAGSFTSRYPQTGTYLDGFEAPSSPRYSFTDPITANMGGPVTFEAPELPEETLMEQEHTDMVQNLRFTLDFARCLMEVAGARGAAVLGEQVDISHPSLLQQHSLVADQISSLSREWSHAEQLVLYLKTAELLSTALHTAMERVKQGKLYPSATVKQVVRRLNELYKSSVASCRSLSTRLERFFSRKHRLMDQITSITAERLLFSHTVQMVQAAALDEMFHQGEASVLRYHKALLLMEGLSLLLTEQDDILSVSKCKECIERRLTALQSGLCV

Target ORF information:

RefSeq Version	XM_022760811.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili unc-51 like autophagy activating kinase 1 (ulk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022760811.1

ORF Insert Sequence:

ATGGAGACGG TGGGGAAATT TGAGTTCAGT CGGAAGGACT TGATAGGACA TGGCGCATTT 
GCTGTCGTCT TCAAAGGCAG GCATCGAGAG AAACATGACT GGGAGGTGGC AGTAAAATGC 
ATAAACAAGA AGAACCTGGC CAAATCCCAG ACACTGTTGG GGAAGGAGAT CAAAATACTC 
AAGGAACTAA AGCATGAAAA CATTGTTGCA TTACTGGACT TTCAGGAAAC TGCCAGCTCA 
GTGTACCTGG TAATGGAGTA CTGCAATGGG GGTGACCTTG CTGACTACTT ACACTCCAAA 
GGCACACTGA GTGAGGACAC TATCCGGGTT TTCCTGCAGC AGATTGCAGG GGCCATGAGG 
GTCCTACAGG CCAAAGGCAT AATCCACAGG GACCTCAAAC CCCAGAATAT TCTGCTCTCC 
TACCCACCAG GACGCAAGTC GCACTCCAAC AACACCTGCA TCAAGATTGC GGACTTTGGC 
TTTGCCAGGT ACCTTCAGAA CAACATGATG GCGGCGACAC TCTGTGGGTC CCCAATGTAC 
ATGGCTCCTG AGGTCATTAT GTCCCAAAAC TATGATGCCA AGGCTGACTT GTGGAGTATA 
GGAACCATAG TGTTTCAGTG CCTGACTGGA AAGGCTCCTT TTCAGGCTAG CAGTCCCCAG 
GACCTCCGGC TGTTCTATGA AAAAAACAAG AATCTCAGCC CCAACATCCC CAGGGAGACT 
TCAAGCCATT TGAGGCACCT GCTGCTGGGA CTGCTGCAGC GCAACCACAA GGACCGCATG 
GACTTTGATG AGTTTTTTGG TCATTCTTTC CTGGAGGCTA GCTCATCAAT GAAAAAGATA 
ACTCCAACTG TGACCATGAC CTGCTTCCCA AGCTCTGCAT CAGCCAGCTC TTGTAGCAGC 
TCTTCCACAT CTCATCTCGC CTCACCACCG CAGTCTCTTG CTGAGGTTCA GCATTTGCGT 
GCCAAAGCTC TGGCCTCTCC TACCCAGGAG GCCACTGGTT TTCTCCTCAA GGACTCGTCT 
GGAGGGGGCG GCAGCAGCAA GAACTCCTCC TCCTGTGACA CAGATGACTT TGTCATGGTG 
CCTGCTCACT TCACGACAGG TGAGCTGACA TGTGAGAGTA AAGTGCTGCA GGACAGCCTG 
ATGAACAGTG GCTCTCTTCT GGCATCTGCT GGTCTGTGTA GCCAAGCCAA AACACCCCCA 
CACTCACCTT CCTACAGTGG CTCTCCAAGT CCTGTCAGGC CCAGTGAGTT CTCGGGAAGT 
AACTACGGTG GTAACTATGG AAACCATGGT CAGTCATTGC CTATTCCAGT CCCCACTCAG 
GTCCAGAACT ACCAGCGCAT GGAGCAGAAT CTCCACTCTA CCAAACAGGA TGGCTCACCA 
CGGCTGTCGA CACCGGTGCG TCGTTGCAGC AGTGGGAGCT CATTGGGCTT TGCTCGGACC 
GGACCTTCAC CATCTTACGG GCAGGGAGCA GTCACCGCCA CCCGCAGGCT CTCCCTGGGA 
GGTGGCAGAA CTTTCCAGCT CTCGCCTCTA GCTCCTCAGC TCACTGAACC CCGGCCGACT 
TCTCAGCAAC ACCCACAGAC GACAGGACTA GGCACACGGC TCCACAGTGC CCCCTGTCTG 
TTGGAGTGTG TCAGTGGTGG CGGGAGGCAG AAGATCAGGA AGCAGTACTC GGACCCTGTG 
GTTGCCCCCT CATCAGGCCT GATGACTGTC CGCCCCCTAC ACTCTTCCCC CAGATTGAGT 
GAGCTGATGC AGCGCAGCCC CCTCCCCACC ATCCTGGGCT CCCCTTCCAG GACCATCCCT 
CCATTTGAAT TCCCTAAGCC TCCCAGCTCT CCAAACCTTG TGACCTTCCT TACACAGCAG 
GGTTTGGTCA TCGGCTCTCC TGGCAGCAGG ACTGCCCTGA CAGAGCCCAG AGAGCCAGGA 
CAACAAGCAC CTACGCAGGT CGCCCAGCCT GCACACTGCA TCCACAGACT GAATGATGAT 
ACCAGGAGCT TCGGAAGGTC TCAGAGTGCT GGTCGTCTGT CTGACATGCT GCTGATGGCT 
GCGTTTGGAC CAGGTGGACC TGTGGGTGAA CGAGGCAGCA CAGAGAACCT GACCTCTGAA 
AAAGCCATAG AAATAACAGC GCCCCCAGGT GCTGGGGTAG GTCTTGCACC TGGATCCAGC 
AGCCCAGCAC AAGTGGTTTT CACTGTGGGC TCTCCTCCCA GTGGCAGCAC CCCACCTCAA 
ACTTCCAGAC AGAGGAAATA CTCAGGCTCG TTCACTTCAG TCAGCCCTGC AGGCTCCTTC 
ACCAGCCGGT ACCCCCAGAC AGGCACCTAT CTGGATGGTT TCGAGGCTCC TTCCAGTCCT 
CGTTACAGTT TCACTGATCC TATCACAGCC AACATGGGAG GCCCCGTAAC CTTTGAGGCT 
CCTGAGCTGC CTGAGGAGAC ACTGATGGAG CAGGAGCATA CAGACATGGT GCAAAACCTG 
CGTTTCACGT TGGATTTTGC ACGTTGTCTA ATGGAAGTGG CCGGGGCCCG TGGTGCTGCG 
GTGTTGGGGG AGCAGGTGGA CATTTCTCAT CCCTCCCTCC TTCAGCAGCA TAGCCTGGTA 
GCAGATCAGA TAAGCTCACT GAGCCGAGAG TGGAGTCATG CAGAACAGCT GGTTCTTTAC 
CTTAAGACTG CAGAACTCTT GTCGACTGCC TTACACACAG CCATGGAGCG AGTTAAACAG 
GGCAAACTCT ACCCATCTGC TACAGTCAAA CAAGTGGTGA GGAGGCTGAA TGAGCTGTAC 
AAATCCAGCG TGGCATCCTG TCGCTCACTC AGCACTCGTC TGGAGCGCTT TTTTTCCAGG 
AAGCACCGTC TTATGGACCA AATCACCTCC ATCACAGCAG AGCGGCTGCT GTTCAGCCAC 
ACTGTGCAGA TGGTTCAGGC TGCTGCGCTG GACGAGATGT TCCACCAGGG GGAAGCATCA 
GTCCTGCGCT ACCATAAAGC TCTGCTGCTG ATGGAGGGCT TGTCTCTGCT GCTCACTGAA 
CAGGATGACA TCCTCAGTGT TAGCAAATGC AAAGAATGCA TTGAACGCCG CCTCACAGCT 
TTGCAGTCAG GACTCTGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ulk1 ( XM_022760812.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022616533.1 Seriola dumerili unc-51 like autophagy activating kinase 1 (ulk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe191272
Clone ID Related Accession (Same CDS sequence)	XM_022760812.1
Accession Version	XM_022760812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	serine/threonine-protein kinase ULK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174620.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGG TGGGGAAATT TGAGTTCAGT CGGAAGGACT TGATAGGACA TGGCGCATTT 
GCTGTCGTCT TCAAAGGCAG GCATCGAGAG AAACATGACT GGGAGGTGGC AGTAAAATGC 
ATAAACAAGA AGAACCTGGC CAAATCCCAG ACACTGTTGG GGAAGGAGAT CAAAATACTC 
AAGGAACTAA AGCATGAAAA CATTGTTGCA TTACTGGACT TTCAGGAAAC TGCCAGCTCA 
GTGTACCTGG TAATGGAGTA CTGCAATGGG GGTGACCTTG CTGACTACTT ACACTCCAAA 
GGCACACTGA GTGAGGACAC TATCCGGGTT TTCCTGCAGC AGATTGCAGG GGCCATGAGG 
GTCCTACAGG CCAAAGGCAT AATCCACAGG GACCTCAAAC CCCAGAATAT TCTGCTCTCC 
TACCCACCAG GACGCAAGTC GCACTCCAAC AACACCTGCA TCAAGATTGC GGACTTTGGC 
TTTGCCAGGT ACCTTCAGAA CAACATGATG GCGGCGACAC TCTGTGGGTC CCCAATGTAC 
ATGGCTCCTG AGGTCATTAT GTCCCAAAAC TATGATGCCA AGGCTGACTT GTGGAGTATA 
GGAACCATAG TGTTTCAGTG CCTGACTGGA AAGGCTCCTT TTCAGGCTAG CAGTCCCCAG 
GACCTCCGGC TGTTCTATGA AAAAAACAAG AATCTCAGCC CCAACATCCC CAGGGAGACT 
TCAAGCCATT TGAGGCACCT GCTGCTGGGA CTGCTGCAGC GCAACCACAA GGACCGCATG 
GACTTTGTAA CTCCAACTGT GACCATGACC TGCTTCCCAA GCTCTGCATC AGCCAGCTCT 
TGTAGCAGCT CTTCCACATC TCATCTCGCC TCACCACCGC AGTCTCTTGC TGAGGTTCAG 
CATTTGCGTG CCAAAGCTCT GGCCTCTCCT ACCCAGGAGG CCACTGGTTT TCTCCTCAAG 
GACTCGTCTG GAGGGGGCGG CAGCAGCAAG AACTCCTCCT CCTGTGACAC AGATGACTTT 
GTCATGGTGC CTGCTCACTT CACGACAGGT GAGCTGACAT GTGAGAGTAA AGTGCTGCAG 
GACAGCCTGA TGAACAGTGG CTCTCTTCTG GCATCTGCTG GTCTGTGTAG CCAAGCCAAA 
ACACCCCCAC ACTCACCTTC CTACAGTGGC TCTCCAAGTC CTGTCAGGCC CAGTGAGTTC 
TCGGGAAGTA ACTACGGTGG TAACTATGGA AACCATGGTC AGTCATTGCC TATTCCAGTC 
CCCACTCAGG TCCAGAACTA CCAGCGCATG GAGCAGAATC TCCACTCTAC CAAACAGGAT 
GGCTCACCAC GGCTGTCGAC ACCGGTGCGT CGTTGCAGCA GTGGGAGCTC ATTGGGCTTT 
GCTCGGACCG GACCTTCACC ATCTTACGGG CAGGGAGCAG TCACCGCCAC CCGCAGGCTC 
TCCCTGGGAG GTGGCAGAAC TTTCCAGCTC TCGCCTCTAG CTCCTCAGCT CACTGAACCC 
CGGCCGACTT CTCAGCAACA CCCACAGACG ACAGGACTAG GCACACGGCT CCACAGTGCC 
CCCTGTCTGT TGGAGTGTGT CAGTGGTGGC GGGAGGCAGA AGATCAGGAA GCAGTACTCG 
GACCCTGTGG TTGCCCCCTC ATCAGGCCTG ATGACTGTCC GCCCCCTACA CTCTTCCCCC 
AGATTGAGTG AGCTGATGCA GCGCAGCCCC CTCCCCACCA TCCTGGGCTC CCCTTCCAGG 
ACCATCCCTC CATTTGAATT CCCTAAGCCT CCCAGCTCTC CAAACCTTGT GACCTTCCTT 
ACACAGCAGG GTTTGGTCAT CGGCTCTCCT GGCAGCAGGA CTGCCCTGAC AGAGCCCAGA 
GAGCCAGGAC AACAAGCACC TACGCAGGTC GCCCAGCCTG CACACTGCAT CCACAGACTG 
AATGATGATA CCAGGAGCTT CGGAAGGTCT CAGAGTGCTG GTCGTCTGTC TGACATGCTG 
CTGATGGCTG CGTTTGGACC AGGTGGACCT GTGGGTGAAC GAGGCAGCAC AGAGAACCTG 
ACCTCTGAAA AAGCCATAGA AATAACAGCG CCCCCAGGTG CTGGGGTAGG TCTTGCACCT 
GGATCCAGCA GCCCAGCACA AGTGGTTTTC ACTGTGGGCT CTCCTCCCAG TGGCAGCACC 
CCACCTCAAA CTTCCAGACA GAGGAAATAC TCAGGCTCGT TCACTTCAGT CAGCCCTGCA 
GGCTCCTTCA CCAGCCGGTA CCCCCAGACA GGCACCTATC TGGATGGTTT CGAGGCTCCT 
TCCAGTCCTC GTTACAGTTT CACTGATCCT ATCACAGCCA ACATGGGAGG CCCCGTAACC 
TTTGAGGCTC CTGAGCTGCC TGAGGAGACA CTGATGGAGC AGGAGCATAC AGACATGGTG 
CAAAACCTGC GTTTCACGTT GGATTTTGCA CGTTGTCTAA TGGAAGTGGC CGGGGCCCGT 
GGTGCTGCGG TGTTGGGGGA GCAGGTGGAC ATTTCTCATC CCTCCCTCCT TCAGCAGCAT 
AGCCTGGTAG CAGATCAGAT AAGCTCACTG AGCCGAGAGT GGAGTCATGC AGAACAGCTG 
GTTCTTTACC TTAAGACTGC AGAACTCTTG TCGACTGCCT TACACACAGC CATGGAGCGA 
GTTAAACAGG GCAAACTCTA CCCATCTGCT ACAGTCAAAC AAGTGGTGAG GAGGCTGAAT 
GAGCTGTACA AATCCAGCGT GGCATCCTGT CGCTCACTCA GCACTCGTCT GGAGCGCTTT 
TTTTCCAGGA AGCACCGTCT TATGGACCAA ATCACCTCCA TCACAGCAGA GCGGCTGCTG 
TTCAGCCACA CTGTGCAGAT GGTTCAGGCT GCTGCGCTGG ACGAGATGTT CCACCAGGGG 
GAAGCATCAG TCCTGCGCTA CCATAAAGCT CTGCTGCTGA TGGAGGGCTT GTCTCTGCTG 
CTCACTGAAC AGGATGACAT CCTCAGTGTT AGCAAATGCA AAGAATGCAT TGAACGCCGC 
CTCACAGCTT TGCAGTCAGG ACTCTGTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022616533.1
CDS	548..3580
Translation	METVGKFEFSRKDLIGHGAFAVVFKGRHREKHDWEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALLDFQETASSVYLVMEYCNGGDLADYLHSKGTLSEDTIRVFLQQIAGAMRVLQAKGIIHRDLKPQNILLSYPPGRKSHSNNTCIKIADFGFARYLQNNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVFQCLTGKAPFQASSPQDLRLFYEKNKNLSPNIPRETSSHLRHLLLGLLQRNHKDRMDFVTPTVTMTCFPSSASASSCSSSSTSHLASPPQSLAEVQHLRAKALASPTQEATGFLLKDSSGGGGSSKNSSSCDTDDFVMVPAHFTTGELTCESKVLQDSLMNSGSLLASAGLCSQAKTPPHSPSYSGSPSPVRPSEFSGSNYGGNYGNHGQSLPIPVPTQVQNYQRMEQNLHSTKQDGSPRLSTPVRRCSSGSSLGFARTGPSPSYGQGAVTATRRLSLGGGRTFQLSPLAPQLTEPRPTSQQHPQTTGLGTRLHSAPCLLECVSGGGRQKIRKQYSDPVVAPSSGLMTVRPLHSSPRLSELMQRSPLPTILGSPSRTIPPFEFPKPPSSPNLVTFLTQQGLVIGSPGSRTALTEPREPGQQAPTQVAQPAHCIHRLNDDTRSFGRSQSAGRLSDMLLMAAFGPGGPVGERGSTENLTSEKAIEITAPPGAGVGLAPGSSSPAQVVFTVGSPPSGSTPPQTSRQRKYSGSFTSVSPAGSFTSRYPQTGTYLDGFEAPSSPRYSFTDPITANMGGPVTFEAPELPEETLMEQEHTDMVQNLRFTLDFARCLMEVAGARGAAVLGEQVDISHPSLLQQHSLVADQISSLSREWSHAEQLVLYLKTAELLSTALHTAMERVKQGKLYPSATVKQVVRRLNELYKSSVASCRSLSTRLERFFSRKHRLMDQITSITAERLLFSHTVQMVQAAALDEMFHQGEASVLRYHKALLLMEGLSLLLTEQDDILSVSKCKECIERRLTALQSGLCV

Target ORF information:

RefSeq Version	XM_022760812.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili unc-51 like autophagy activating kinase 1 (ulk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022760812.1

ORF Insert Sequence:

ATGGAGACGG TGGGGAAATT TGAGTTCAGT CGGAAGGACT TGATAGGACA TGGCGCATTT 
GCTGTCGTCT TCAAAGGCAG GCATCGAGAG AAACATGACT GGGAGGTGGC AGTAAAATGC 
ATAAACAAGA AGAACCTGGC CAAATCCCAG ACACTGTTGG GGAAGGAGAT CAAAATACTC 
AAGGAACTAA AGCATGAAAA CATTGTTGCA TTACTGGACT TTCAGGAAAC TGCCAGCTCA 
GTGTACCTGG TAATGGAGTA CTGCAATGGG GGTGACCTTG CTGACTACTT ACACTCCAAA 
GGCACACTGA GTGAGGACAC TATCCGGGTT TTCCTGCAGC AGATTGCAGG GGCCATGAGG 
GTCCTACAGG CCAAAGGCAT AATCCACAGG GACCTCAAAC CCCAGAATAT TCTGCTCTCC 
TACCCACCAG GACGCAAGTC GCACTCCAAC AACACCTGCA TCAAGATTGC GGACTTTGGC 
TTTGCCAGGT ACCTTCAGAA CAACATGATG GCGGCGACAC TCTGTGGGTC CCCAATGTAC 
ATGGCTCCTG AGGTCATTAT GTCCCAAAAC TATGATGCCA AGGCTGACTT GTGGAGTATA 
GGAACCATAG TGTTTCAGTG CCTGACTGGA AAGGCTCCTT TTCAGGCTAG CAGTCCCCAG 
GACCTCCGGC TGTTCTATGA AAAAAACAAG AATCTCAGCC CCAACATCCC CAGGGAGACT 
TCAAGCCATT TGAGGCACCT GCTGCTGGGA CTGCTGCAGC GCAACCACAA GGACCGCATG 
GACTTTGTAA CTCCAACTGT GACCATGACC TGCTTCCCAA GCTCTGCATC AGCCAGCTCT 
TGTAGCAGCT CTTCCACATC TCATCTCGCC TCACCACCGC AGTCTCTTGC TGAGGTTCAG 
CATTTGCGTG CCAAAGCTCT GGCCTCTCCT ACCCAGGAGG CCACTGGTTT TCTCCTCAAG 
GACTCGTCTG GAGGGGGCGG CAGCAGCAAG AACTCCTCCT CCTGTGACAC AGATGACTTT 
GTCATGGTGC CTGCTCACTT CACGACAGGT GAGCTGACAT GTGAGAGTAA AGTGCTGCAG 
GACAGCCTGA TGAACAGTGG CTCTCTTCTG GCATCTGCTG GTCTGTGTAG CCAAGCCAAA 
ACACCCCCAC ACTCACCTTC CTACAGTGGC TCTCCAAGTC CTGTCAGGCC CAGTGAGTTC 
TCGGGAAGTA ACTACGGTGG TAACTATGGA AACCATGGTC AGTCATTGCC TATTCCAGTC 
CCCACTCAGG TCCAGAACTA CCAGCGCATG GAGCAGAATC TCCACTCTAC CAAACAGGAT 
GGCTCACCAC GGCTGTCGAC ACCGGTGCGT CGTTGCAGCA GTGGGAGCTC ATTGGGCTTT 
GCTCGGACCG GACCTTCACC ATCTTACGGG CAGGGAGCAG TCACCGCCAC CCGCAGGCTC 
TCCCTGGGAG GTGGCAGAAC TTTCCAGCTC TCGCCTCTAG CTCCTCAGCT CACTGAACCC 
CGGCCGACTT CTCAGCAACA CCCACAGACG ACAGGACTAG GCACACGGCT CCACAGTGCC 
CCCTGTCTGT TGGAGTGTGT CAGTGGTGGC GGGAGGCAGA AGATCAGGAA GCAGTACTCG 
GACCCTGTGG TTGCCCCCTC ATCAGGCCTG ATGACTGTCC GCCCCCTACA CTCTTCCCCC 
AGATTGAGTG AGCTGATGCA GCGCAGCCCC CTCCCCACCA TCCTGGGCTC CCCTTCCAGG 
ACCATCCCTC CATTTGAATT CCCTAAGCCT CCCAGCTCTC CAAACCTTGT GACCTTCCTT 
ACACAGCAGG GTTTGGTCAT CGGCTCTCCT GGCAGCAGGA CTGCCCTGAC AGAGCCCAGA 
GAGCCAGGAC AACAAGCACC TACGCAGGTC GCCCAGCCTG CACACTGCAT CCACAGACTG 
AATGATGATA CCAGGAGCTT CGGAAGGTCT CAGAGTGCTG GTCGTCTGTC TGACATGCTG 
CTGATGGCTG CGTTTGGACC AGGTGGACCT GTGGGTGAAC GAGGCAGCAC AGAGAACCTG 
ACCTCTGAAA AAGCCATAGA AATAACAGCG CCCCCAGGTG CTGGGGTAGG TCTTGCACCT 
GGATCCAGCA GCCCAGCACA AGTGGTTTTC ACTGTGGGCT CTCCTCCCAG TGGCAGCACC 
CCACCTCAAA CTTCCAGACA GAGGAAATAC TCAGGCTCGT TCACTTCAGT CAGCCCTGCA 
GGCTCCTTCA CCAGCCGGTA CCCCCAGACA GGCACCTATC TGGATGGTTT CGAGGCTCCT 
TCCAGTCCTC GTTACAGTTT CACTGATCCT ATCACAGCCA ACATGGGAGG CCCCGTAACC 
TTTGAGGCTC CTGAGCTGCC TGAGGAGACA CTGATGGAGC AGGAGCATAC AGACATGGTG 
CAAAACCTGC GTTTCACGTT GGATTTTGCA CGTTGTCTAA TGGAAGTGGC CGGGGCCCGT 
GGTGCTGCGG TGTTGGGGGA GCAGGTGGAC ATTTCTCATC CCTCCCTCCT TCAGCAGCAT 
AGCCTGGTAG CAGATCAGAT AAGCTCACTG AGCCGAGAGT GGAGTCATGC AGAACAGCTG 
GTTCTTTACC TTAAGACTGC AGAACTCTTG TCGACTGCCT TACACACAGC CATGGAGCGA 
GTTAAACAGG GCAAACTCTA CCCATCTGCT ACAGTCAAAC AAGTGGTGAG GAGGCTGAAT 
GAGCTGTACA AATCCAGCGT GGCATCCTGT CGCTCACTCA GCACTCGTCT GGAGCGCTTT 
TTTTCCAGGA AGCACCGTCT TATGGACCAA ATCACCTCCA TCACAGCAGA GCGGCTGCTG 
TTCAGCCACA CTGTGCAGAT GGTTCAGGCT GCTGCGCTGG ACGAGATGTT CCACCAGGGG 
GAAGCATCAG TCCTGCGCTA CCATAAAGCT CTGCTGCTGA TGGAGGGCTT GTCTCTGCTG 
CTCACTGAAC AGGATGACAT CCTCAGTGTT AGCAAATGCA AAGAATGCAT TGAACGCCGC 
CTCACAGCTT TGCAGTCAGG ACTCTGTGTT TAA